SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_148219526.1 NCBI__GCF_000010725.1:WP_148219526.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
50% identity, 99% coverage: 1:711/721 of query aligns to 2:713/715 of 1wdlA

query
sites
1wdlA

M

I

F

Y

Q

E

G

G

N

K

T

A

L

I

Q

T

L

V

H

T

A

A

L

L

G

E

D

S

G

G

L

I

A

V

E

E

L

L

R

K

F

F

D

D

R

L

A

K

G

G

E

E

S

S

V

V

N

N

K

K

L

F

D

N

R

R

L

L

A

T

L

L

D

N

E

E

L

L

N

R

R

Q

A

A

L

V

C

D

L

A

V

I

R

K

E

A

D

D

R

A

T

S

V

V

T

K

G

G

L

V

L

I

I

V

T

S

S

S

G

G

K

K

D

D

S

V

F

F

I

I

V

V

G

G

A
|
A

D

D

I

I

F

T

E

E

F

F

T

V

D

E

L

N

F

F

G

K

M

L

A

P

E

D

D

A

E

E

I

L

A

I

I

A

H

G

N
|
N

A

L

G

E

Q

A

S
x
N

A

K

V

I

I

F

T

S

A

D

L

F

A

E

D

D

L

L

P

N

V

V

P

P

T

T

V

V

V

A

A

A

V

I

N

N

G

G

L

I

A

A

L

L

G

G

G
|
G

G

G

L

L

E
|
E

L

M

A

C

L

L

A

A

A

A

D

D

V

F

R

R

M

V

L

M

S

A

D

D

S

S

A

A

R

K

I

I

G

G

L

L

P
|
P

E
|
E

V

V

G

K

L

L

G

G

I

I

F

Y

P
|
P

G
|
G

F

F

G

G

G

G

T

T

V

V

R

R

L

L

P

P

R

R

L

L

I

I

G

G

V

V

E

D

A

N

A

A

L

V

Q

E

W

W

I

I

M

A

S

S

G

G

T

K

P

E

R

N

K

R

A

A

D

E

D

D

A

A

L

L

R

K

A

V

G

S

A

A

V

V

D

D

A

A

V

V

V

V

P

T

P

A

P

D

E

K

L

L

R

G

E

A

A

A

A

A

L

L

E

D

R

L

L

I

H

K

G

R

L

A

V

I

A

S

S

G

P

E

-

L

D

D

W

Y

S

K

E

A

R

K

R

R

K

Q

E

P

A

K

L

L

S

E

P

K

V

L

-

K

V

L

R

N

A

A

G

I

E

E

D

Q

L

M

A

M

P

A

I

F

F

E

A

T

A

A

A

K

K

G

A

F

K

V

A

A

R

G

K

Q

T

A

G

G

P

P

H

N

L

Y

P

P

A

A

S

P

L

V

A

E

V

A

V

I

E

K

L

T

I

I

E

Q

S

K

A

A

A

A

G

N

V

F

D

G

R

R

D

D

A

K

A

A

L

L

A

E

L

V

E

E

A

A

R

A

A

G

F

F

A

A

H

K

I

L

A

A

R

K

T

T

P

S

A

A

A

S

R

N

A

C

L

L

V

I

A

G

V

L

F

F

I

L

N

N

D

D

Q

Q

A

E

V

L

K

K

K

K

T

K

T

A

K

K

Q

V

Q

Y

T

D

R

K

A

I

A

A

R

K

P

D

V

V

S

K

R

Q

A

A

A

A

V

V

L

L

G

G

A
|
A

G

G

I
|
I

M
|
M

G

G

G

G

G

G

I

I

A

A

Y

Y

Q

Q

S

S

A

A

V

S

C

K

G

G

I

T

P

P

V

I

I

L

M

M

K

K

D
|
D

I
|
I

A

N

Q

E

S

H

A

G

L

I

D

E

L

Q

G

G

M

L

T

A

Q

E

A

A

G

A

A

K

L

L

L

L

A

V

K

G

R

R

V

V

E

D

T

K

G

G

A

R

L

M

S

T

S

P

A

A

K

K

A

M

D

A

G

E

I

V

L

L

A

N

S

G

I

I

R

R

P

P

G

T

L

L

T

S

Y

Y

D

G

G

D

I

F

A

G

E

N

A

V

D

D

V

L

I

V

V

V

E

E

A
|
A

V
|
V

V

V

E
|
E

N

N

I

P

A

K

I

V

K

K

R

Q

A

A

V

V

L

L

A

A

E

E

L

V

E

E

E

N

H

H

I

V

R

R

P

E

D

D

A

A

V

I

V

L

A

A

T

S

N
|
N

T
|
T

S
|
S

S

T

L

I

L

S

V

I

A

S

D

L

L

L

A

A

R

K

D

A

L

L

E

K

W

R

P

P

E

E

N

N

F

F

V

V

G

G

M

M

H
|
H

F

F

F

F

N

N

P

P

V

V

H

H

R

M

M

M

P

P

L

L

V

V

E
|
E

V

V

I

I

Q

R

G

G

P

E

H

K

T

S

S

S

P

D

E

L

A

A

V

V

A

A

T

T

I

T

V

V

G

A

Y

Y

A

A

S

K

A

K

M

M

G

G

K

K

T

N

P

P

V

I

V

V

V

V

R

N

D

D

C

C

P

P

G

G

F

F

L

L

V

V

N
|
N

R

R

I

V

L

L

A

F

A

P

N

Y

V

F

L

G

G

G

F

F

L

A

R

K

L

L

I

V

H

S

D

A

G

G

A

V

D

D

F

F

L

V

R

R

I

I

D

D

A

K

A

V

M

M

E

E

G

K

F

F

G

G

W

W

P

P

M

M

G

G

P

P

A

A

W

Y

L

L

Q

M

D

D

V

V

I

V

G

G

M

I

D

D

T

T

S

G

R

H

H

H

V

G

I

R

E

D

V

V

I

M

A

A

A

E

G

G

Y

F

P

P

E

D

R

R

M

M

R

K

V

D

E

D

F

R

A

R

T

S

A

A

L

I

D

D

V

A

M

L

V

Y

E

E

S

A

K

K

R

R

F

L

G

G

Q

Q

K

K

S

N

G

G

A

K

G

G

F

F

Y

Y

R

A

Y

Y

E

E

A

A

D

D

A

K

A

K

G

G

H

K

Q

Q

R

K

K

K

V

L

V

V

D

D

P

S

D

S

V

V

G

-

A

-

L

-

L

L

A

E

T

V

V

L

Q

K

P

P

-

I

-

V

G

Y

G

E

S

Q

R

R

I

D

I

V

S

T

D

D

E

E

E

D

I

I

V

I

Q

N

R

W

T

M

M

M

L

I

P

P

M

L

I

C

L

L

E

E

T

T

A

V

R

R

C

C

L

L

E

E

D

D

G

G

V

I

V

V

G

E

S

T

A

A

A

A

E

E

A

A

D

D

M

M

A

G

L

L

I

V

L

Y

G

G

I

I

G

G

F

F

P

P

R

L

H

F

L

R

G

G

G

G

A

A

L

L

H

R

Y

Y

A

I

D

D

E

S

L

I

G

G

A

V

K

A

T

E

I

F

M

V

E

A

W

L

C

A

D

D

R

Q

Y

Y

A

A

N

E

L

L

G

G

P

A

L

L

Y

Y

H

H

P

P

T

T

P

A

R

K

M

L

R

R

R

E

L

M

A

A

V

K

D

N

G

G

A

Q

G

S

W

F

active site: A69 (= A68), N89 (= N88), N93 (≠ S92), G117 (= G116), E120 (= E119), P139 (= P138), E140 (= E139), P147 (= P146), G148 (= G147), S430 (= S427), H451 (= H448), E463 (= E460), N501 (= N498)
binding nicotinamide-adenine-dinucleotide: A322 (= A319), I324 (= I321), M325 (= M322), D344 (= D341), I345 (= I342), A400 (= A397), V401 (= V398), E403 (= E400), N428 (= N425), T429 (= T426), S430 (= S427)

P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
50% identity, 99% coverage: 1:711/721 of query aligns to 2:713/715 of P28793

query
sites
P28793

M

I

F

Y

Q

E

G

G

N

K

T

A

L

I

Q

T

L

V

H

T

A

A

L

L

G

E

D

S

G

G

L

I

A

V

E

E

L

L

R

K

F

F

D

D

R

L

A

K

G

G

E

E

S

S

V

V

N

N

K

K

L

F

D

N

R

R

L

L

A

T

L

L

D

N

E

E

L

L

N

R

R

Q

A

A

L

V

C

D

L

A

V

I

R

K

E

A

D

D

R

A

T

S

V

V

T

K

G

G

L

V

L

I

I

V

T

S

S

S

G

G

K

K

D

D

S

V

F

F

I

I

V

V

G

G

A

A

D

D

I

I

F

T

E

E

F

F

T

V

D

E

L

N

F

F

G

K

M

L

A

P

E

D

D

A

E

E

I

L

A

I

I

A

H

G

N

N

A

L

G

E

Q

A

S

N

A

K

V

I

I

F

T

S

A

D

L

F

A

E

D

D

L

L

P

N

V

V

P

P

T

T

V

V

V

A

A

A

V

I

N

N

G

G

L

I

A

A

L

L

G

G

G

G

G

G

L

L

E

E

L

M

A

C

L

L

A

A

A

A

D

D

V

F

R

R

M

V

L

M

S

A

D

D

S

S

A

A

R

K

I

I

G

G

L

L

P

P

E

E

V

V

G

K

L

L

G

G

I

I

F

Y

P

P

G

G

F

F

G

G

G

G

T

T

V

V

R

R

L

L

P

P

R

R

L

L

I

I

G

G

V

V

E

D

A

N

A

A

L

V

Q

E

W

W

I

I

M

A

S

S

G

G

T

K

P

E

R

N

K

R

A

A

D

E

D

D

A

A

L

L

R

K

A

V

G

S

A

A

V

V

D

D

A

A

V

V

V

V

P

T

P

A

P

D

E

K

L

L

R

G

E

A

A

A

A

A

L

L

E

D

R

L

L

I

H

K

G

R

L

A

V

I

A

S

S

G

P

E

-

L

D

D

W

Y

S

K

E

A

R

K

R

R

K

Q

E

P

A

K

L

L

S

E

P

K

V

L

-

K

V

L

R

N

A

A

G

I

E

E

D

Q

L

M

A

M

P

A

I

F

F

E

A

T

A

A

A

K

K

G

A

F

K

V

A

A

R

G

K

Q

T

A

G

G

P

P

H

N

L

Y

P

P

A

A

S

P

L

V

A

E

V

A

V

I

E

K

L

T

I

I

E

Q

S

K

A

A

A

A

G

N

V

F

D

G

R

R

D

D

A

K

A

A

L

L

A

E

L

V

E

E

A

A

R

A

A

G

F

F

A

A

H

K

I

L

A

A

R

K

T

T

P

S

A

A

A

S

R

N

A

C

L

L

V

I

A

G

V

L

F

F

I

L

N

N

D
|
D

Q

Q

A

E

V

L

K

K

K

K

T

K

T

A

K

K

Q

V

Q

Y

T

D

R

K

A

I

A

A

R

K

P

D

V

V

S

K

R

Q

A

A

A

A

V

V

L

L

G

G

A

A

G

G

I

I

M
|
M

G

G

G

G

G

G

I

I

A

A

Y

Y

Q

Q

S

S

A

A

V

S

C

K

G

G

I

T

P

P

V

I

I

L

M

M

K

K

D
|
D

I

I

A

N

Q

E

S

H

A

G

L

I

D

E

L

Q

G

G

M

L

T

A

Q

E

A

A

G

A

A

K

L

L

L

L

A

V

K

G

R

R

V

V

E

D

T

K

G

G

A

R

L

M

S

T

S

P

A

A

K

K

A

M

D

A

G

E

I

V

L

L

A

N

S

G

I

I

R

R

P

P

G

T

L

L

T

S

Y

Y

D

G

G

D

I

F

A

G

E

N

A

V

D

D

V

L

I

V

V

V

E

E

A

A

V
|
V

V
|
V

E
|
E

N

N

I

P

A

K

I

V

K
|
K

R

Q

A

A

V

V

L

L

A

A

E

E

L

V

E

E

E

N

H

H

I

V

R

R

P

E

D

D

A

A

V

I

V

L

A

A

T

S

N

N

T

T

S
|
S

S

T

L

I

L

S

V

I

A

S

D

L

L

L

A

A

R

K

D

A

L

L

E

K

W

R

P

P

E

E

N

N

F

F

V

V

G

G

M

M

H

H

F

F

F

F

N
|
N

P

P

V

V

H

H

R

M

M

M

P

P

L

L

V

V

E

E

V

V

I

I

Q

R

G

G

P

E

H

K

T

S

S

S

P

D

E

L

A

A

V

V

A

A

T

T

I

T

V

V

G

A

Y

Y

A

A

S

K

A

K

M

M

G

G

K

K

T

N

P

P

V

I

V

V

V

V

R

N

D

D

C

C

P

P

G

G

F

F

L

L

V

V

N
|
N

R

R

I

V

L

L

A

F

A

P

N

Y

V

F

L

G

G

G

F

F

L

A

R

K

L

L

I

V

H

S

D

A

G

G

A

V

D

D

F

F

L

V

R

R

I

I

D

D

A

K

A

V

M

M

E

E

G

K

F

F

G

G

W

W

P

P

M

M

G

G

P

P

A

A

W

Y

L

L

Q

M

D

D

V

V

I

V

G

G

M

I

D

D

T

T

S

G

R

H

H

H

V

G

I

R

E

D

V

V

I

M

A

A

A

E

G

G

Y

F

P

P

E

D

R

R

M

M

R

K

V

D

E

D

F

R

A

R

T

S

A

A

L

I

D

D

V

A

M

L

V

Y

E

E

S

A

K

K

R

R

F

L

G

G

Q

Q

K

K

S

N

G

G

A

K

G

G

F

F

Y

Y

R

A

Y

Y

E

E

A

A

D

D

A

K

A

K

G

G

H

K

Q

Q

R

K

K

K

V

L

V

V

D

D

P

S

D

S

V

V

G

-

A

-

L

-

L

L

A

E

T

V

V

L

Q

K

P

P

-

I

-

V

G

Y

G

E

S

Q

R

R

I

D

I

V

S

T

D

D

E

E

E

D

I

I

V

I

Q

N

R

W

T

M

M

M

L

I

P

P

M

L

I

C

L

L

E

E

T

T

A

V

R

R

C

C

L

L

E

E

D

D

G

G

V

I

V

V

G

E

S

T

A

A

A

A

E

E

A

A

D

D

M

M

A

G

L

L

I

V

L
x
Y

G

G

I

I

G

G

F

F

P

P

R

L

H

F

L

R

G

G

G

G

A

A

L

L

H

R

Y

Y

A

I

D

D

E

S

L

I

G

G

A

V

K

A

T

E

I

F

M

V

E

A

W

L

C

A

D

D

R

Q

Y

Y

A

A

N

E

L

L

G

G

P

A

L

L

Y

Y

H

H

P

P

T

T

P

A

R

K

M

L

R

R

R

E

L

M

A

A

V

K

D

N

G

G

A

Q

G

S

W

F

D297 (= D294) binding substrate
M325 (= M322) binding NAD(+)
D344 (= D341) binding NAD(+)
VVE 401:403 (= VVE 398:400) binding NAD(+)
K408 (= K405) binding NAD(+)
S430 (= S427) binding NAD(+)
N454 (= N451) binding NAD(+)
N501 (= N498) binding substrate
Y660 (≠ L658) binding substrate

1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
49% identity, 99% coverage: 1:711/721 of query aligns to 2:705/707 of 1wdmA

query
sites
1wdmA

M

I

F

Y

Q

E

G

G

N

K

T

A

L

I

Q

T

L

V

H

T

A

A

L

L

G

E

D

S

G

G

L

I

A

V

E

E

L

L

R

K

F

F

D

D

R

L

A

K

G

G

E

E

S

S

V

V

N

N

K

K

L

F

D

N

R

R

L

L

A

T

L

L

D

N

E

E

L

L

N

R

R

Q

A

A

L

V

C

D

L

A

V

I

R

K

E

A

D

D

R

A

T

S

V

V

T

K

G

G

L

V

L

I

I

V

T

S

S

S

G

G

K

K

D

D

S

V

F

F

I

I

V

V

G

G

A
|
A

D

D

I

I

F

T

E

E

F

F

T

V

D

E

L

N

F

F

G

K

M

L

A

P

E

D

D

A

E

E

I

L

A

I

I

A

H

G

N
|
N

A

L

G

E

Q

A

S
x
N

A

K

V

I

I

F

T

S

A

D

L

F

A

E

D

D

L

L

P

N

V

V

P

P

T

T

V

V

V

A

A

A

V

I

N

N

G

G

L

I

A

A

L

L

G

G

G
|
G

G

G

L

L

E
|
E

L

M

A

C

L

L

A

A

A

A

D

D

V

F

R

R

M

V

L

M

S

A

D

D

S

S

A

A

R

K

I

I

G

G

L

L

P
|
P

E
|
E

V

V

G
x
K

L

L

G

G

I

I

F

Y

P
|
P

G
|
G

F

F

G

G

G

G

T

T

V

V

R

R

L

L

P

P

R

R

L

L

I

I

G

G

V

V

E

D

A

N

A

A

L

V

Q

E

W

W

I

I

M

A

S

S

G

G

T

K

P

E

R

N

K

R

A

A

D

E

D

D

A

A

L

L

R

K

A

V

G

S

A

A

V

V

D

D

A

A

V

V

V

V

P

T

P

A

P

D

E

K

L

L

R

G

E

A

A

A

A

A

L

L

E

D

R

L

L

I

H

K

G

R

L

A

V

I

A

S

S

G

P

E

-

L

D

D

W

Y

S

K

E

A

R

K

R

R

K

Q

E

P

A

K

L

L

S

E

P

K

V

L

-

K

V

L

R

N

A

A

G

I

E

E

D

Q

L

M

A

M

P

A

I

F

F

E

A

T

A

A

A

K

K

G

A

F

K

V

A

A

R

G

K

Q

T

A

G

G

P

P

H

N

L

Y

P

P

A

A

S

P

L

V

A

E

V

A

V

I

E

K

L

T

I

I

E

Q

S

K

A

A

A

A

G

N

V

F

D

G

R

R

D

D

A

K

A

A

L

L

A

E

L

V

E

E

A

A

R

A

A

G

F

F

A

A

H

K

I

L

A

A

R

K

T

T

P

S

A

A

A

S

R

N

A

C

L

L

V

I

A

G

V

L

F

F

I

L

N

N

D
|
D

Q

Q

A

E

V

L

K

K

K

K

T

K

T

A

K

K

Q

V

Q

Y

T

D

R

K

A

I

A

A

R

K

P

D

V

V

S

K

R

Q

A

A

A

A

V

V

L

L

G
|
G

A
|
A

G

G

I
|
I

M
|
M

G

G

G

G

G

G

I

I

A

A

Y

Y

Q

Q

S

S

A

A

V

S

C

K

G

G

I

T

P

P

V

I

I

L

M

M

K

K

D
|
D

I

I

A

N

Q

E

S

H

A

G

L

I

D

E

L

Q

G

G

M

L

T

A

Q

E

A

A

G

A

A

K

L

L

L

L

A

V

K

G

R

R

V

V

E

D

T

K

G

G

A

R

L

M

S

T

S

P

A

A

K

K

A

M

D

A

G

E

I

V

L

L

A

N

S

G

I

I

R

R

P

P

G

T

L

L

T

S

Y

Y

D

G

G

D

I

F

A

G

E

N

A

V

D

D

V

L

I

V

V

V

E

E

A

A

V
|
V

V

V

E
|
E

N

N

I

P

A

K

I

V

K

K

R

Q

A

A

V

V

L

L

A

A

E

E

L

V

E

E

E

N

H

H

I

V

R

R

P

E

D

D

A

A

V

I

V

L

A

A

T

S

N
|
N

T

T

S
|
S

S

T

L

I

L

S

V

I

A

S

D

L

L

L

A

A

R

K

D

A

L

L

E

K

W

R

P

P

E

E

N

N

F

F

V

V

G

G

M

M

H
|
H

F

F

F

F

N
|
N

P

P

V

V

H

H

R

M

M
|
M

P

P

L

L

V

V

E
|
E

V

V

I

I

Q

R

G

G

P

E

H

K

T

S

S

S

P

D

E

L

A

A

V

V

A

A

T

T

I

T

V

V

G

A

Y

Y

A

A

S

K

A

K

M

M

G

G

K

K

T

N

P

P

V

I

V

V

V

V

R

N

D

D

C

C

P

P

G

G

F

F

L

L

V

V

N
|
N

R

R

I

V

L

L

A

F

A

P

N

Y

V

F

L

G

G

G

F

F

L

A

R

K

L

L

I

V

H

S

D

A

G

G

A

V

D

D

F

F

L

V

R

R

I

I

D

D

A

K

A

V

M

M

E

E

G

K

F

F

G

G

W

W

P
|
P

M

M

G

G

P

P

A

A

W

Y

L

L

Q

M

D

D

V

V

I

V

G

G

M

I

D

D

T

T

S

G

R

H

H

H

V

G

I

R

E

D

V

V

I

M

A

A

A

E

G

G

Y

F

P

P

E

D

R

R

M

M

R

K

V

D

E

D

F

R

A

R

T

S

A

A

L

I

D

D

V

A

M

L

V

Y

E

E

S

A

K

K

R

R

F

L

G

G

Q

Q

K

K

S

N

G

G

A

K

G

G

F

F

Y

Y

R

A

Y

Y

E

-

A

-

D

-

A

-

A

-

G

-

H

-

Q

-

R

K

K

K

V

L

V

V

D

D

P

S

D

S

V

V

G

-

A

-

L

-

L

L

A

E

T

V

V

L

Q

K

P

P

-

I

-

V

G

Y

G

E

S

Q

R

R

I

D

I

V

S

T

D

D

E

E

E

D

I

I

V

I

Q

N

R

W

T

M

M

M

L

I

P

P

M

L

I

C

L

L

E

E

T

T

A

V

R

R

C

C

L

L

E

E

D

D

G

G

V

I

V

V

G

E

S

T

A

A

A

A

E

E

A

A

D

D

M

M

A

G

L

L

I

V

L
x
Y

G

G

I

I

G

G

F

F

P

P

R
x
L

H

F

L

R

G

G

G

G

A

A

L

L

H

R

Y

Y

A

I

D

D

E

S

L

I

G

G

A

V

K

A

T

E

I

F

M

V

E

A

W

L

C

A

D

D

R

Q

Y

Y

A

A

N

E

L

L

G

G

P

A

L

L

Y

Y

H

H

P

P

T

T

P

A

R

K

M

L

R

R

R

E

L

M

A

A

V

K

D

N

G

G

A

Q

G

S

W

F

active site: A69 (= A68), N89 (= N88), N93 (≠ S92), G117 (= G116), E120 (= E119), P139 (= P138), E140 (= E139), P147 (= P146), G148 (= G147), S430 (= S427), H451 (= H448), E463 (= E460), N501 (= N498)
binding acetyl coenzyme *a: K142 (≠ G141), D297 (= D294), M459 (= M456), N501 (= N498), P534 (= P531), Y652 (≠ L658), L658 (≠ R664)
binding nicotinamide-adenine-dinucleotide: G321 (= G318), A322 (= A319), I324 (= I321), M325 (= M322), D344 (= D341), V401 (= V398), E403 (= E400), N428 (= N425), S430 (= S427), N454 (= N451)

P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
49% identity, 98% coverage: 1:705/721 of query aligns to 2:707/729 of P21177

query
sites
P21177

M

L

F

Y

Q

K

G

G

N

D

T

T

L

L

Q

Y

L

L

H

D

A

W

L

L

G

E

D

D

G

G

L

I

A

A

E

E

L

L

R

V

F

F

D

D

R

A

A

P

G

G

E

-

S

S

V

V

N

N

K

K

L

L

D

D

R

T

L

A

A

T

L

V

D

A

E

S

L

L

N

G

R

E

A

A

L

I

C

G

L

V

V

L

R

E

E

Q

D

Q

R

S

T

D

V

L

T

K

G

G

L

L

L

L

I

L

T

R

S

S

G

N

K

K

D

A

S

A

F

F

I

I

V

V

G

G

A

A

D

D

I

I

F

T

E

E

F

F

T

L

D

S

L

L

F

F

G

L

M

V

A

P

E

E

D

E

E

Q

I

L

A

S

I

Q

H

W

N

L

A

H

G

F

Q

A

S

N

A

S

V

V

I

F

T

N

A

R

L

L

A

E

D

D

L

L

P

P

V

V

P

P

T

T

V

I

V

A

A

A

V

V

N

N

G

G

L

Y

A

A

L

L

G

G

G
|
G

G

G

L

C

E

E

L

C

A

V

L

L

A

A

A

T

D

D

V

Y

R

R

M

L

L

A

S

T

D

P

S

D

A

L

R

R

I

I

G

G

L

L

P

P

E

E

V

T

G

K

L

L

G

G

I

I

F

M

P

P

G

G

F

F

G

G

G

G

T

S

V

V

R

R

L

M

P

P

R

R

L

M

I

L

G

G

V

A

E

D

A

S

A

A

L

L

Q

E

W

I

I

I

M

A

S

A

G

G

T

K

P

D

R

V

K

G

A

A

D

D

D

Q

A

A

L

L

R

K

A

I

G

G

A

L

V

V

D

D

A

G

V

V

V

V

P

K

P

A

P

E

E

K

L

L

R

V

E

E

A

G

A

A

L

K

E

A

R

V

L

L

-

R

H

Q

G

A

L

I

V

N

A

G

S

D

P

L

D

D

W

W

S

K

E

A

R

K

R

R

K

Q

E

P

A

K

L

L

S

E

P

P

V

L

V

K

R

L

A

S

G

K

E

I

D

E

L

A

A

T

P

M

I

S

F

F

A

T

A

I

A

A

K

K

A

G

K

M

A

V

R

A

K

Q

T

T

-

A

G

G

P

K

H

H

L

Y

P

P

A

A

S

P

L

I

A

T

V

A

V

V

E

K

L

T

I

I

E

E

S

A

A

A

A

A

G

R

V

F

D

G

R

R

D

E

A

E

A

A

L

L

A

N

L

L

E

E

A

N

R

K

A

S

F

F

A

V

H

P

I

L

A

A

R

H

T

T

P

N

A

E

A

A

R

R

A

A

L

L

V

V

A

G

V

I

F

F

I

L

N

N

D

D

Q

Q

A

Y

V

V

K

K

K

G

T

K

T

A

K

K

Q

K

Q

L

T

T

R

K

A

D

A

V

R

E

P

T

V

P

S

K

R

Q

A

A

A

A

V

V

L

L

G

G

A

A

G
|
G

I

I

M

M

G

G

G

G

G

G

I

I

A

A

Y

Y

Q

Q

S

S

A

A

V

W

C

K

G

G

I

V

P

P

V

V

I

V

M

M

K

K

D

D

I

I

A

N

Q

D

S

K

A

S

L

L

D

T

L

L

G

G

M

M

T

T

Q

E

A

A

G

A

A

K

L

L

L

L

A

N

K

K

R

Q

V

L

E

E

T

R

G

G

A

K

L

I

S

D

S

G

A

L

K

K

A

L

D

A

G

G

I

V

L

I

A

S

S

T

I

I

R

H

P

P

G

T

L

L

T

D

Y

Y

D

A

G

G

I

F

A

D

E

R

A

V

D

D

V

I

I

V

V

V

E

E

A

A

V

V

V

V

E

E

N

N

I

P

A

K

I

V

K

K

R

K

A

A

V

V

L

L

A

A

E

E

L

T

E

E

E

Q

H

K

I

V

R

R

P

Q

D

D

A

T

V

V

V

L

A

A

T

S

N

N

T

T

S

S

S

T

L

I

L

P

V

I

A

S

D

E

L

L

A

A

R

N

D

A

L

L

E

E

W

R

P

P

E

E

N

N

F

F

V

C

G

G

M

M

H
|
H

F

F

F

F

N

N

P

P

V

V

H

H

R

R

M

M

P

P

L

L

V

V

E

E

V

I

I

I

Q

R

G

G

P

E

H

K

T

S

S

S

P

D

E

E

A

T

V

I

A

A

T

K

I

V

V

V

G

A

Y

W

A

A

S

S

A

K

M

M

G

G

K

K

T

T

P

P

V

I

V

V

V

V

R

N

D

D

C

C

P

P

G

G

F

F

L

F

V

V

N

N

R

R

I

V

L

L

A

F

A

P

N

Y

V

F

L

A

G

G

F

F

L

S

R

Q

L

L

I

L

H

R

D

D

G

G

A

A

D

D

F

F

L

R

R

K

I

I

D

D

A

K

A

V

M

M

E

E

G

K

-

Q

F

F

G

G

W

W

P

P

M

M

G

G

P

P

A

A

W

Y

L

L

Q

L

D

D

V

V

I

V

G

G

M

I

D

D

T

T

S

A

R

H

H

H

V

A

I

Q

E

A

V

V

I

M

A

A

A

A

G

G

Y

F

P

P

E

Q

R

R

M

M

R

Q

V

K

E

D

F

Y

A

R

T

D

A

A

L

I

D

D

V

A

M

L

V

F

E

D

S

A

K

N

R

R

F

F

G

G

Q

Q

K

K

S

N

G

G

A

L

G

G

F

F

Y

W

R

R

Y

Y

E

K

A

E

D

D

A

S

A

K

G

G

H

K

Q

P

R

K

K

K

V

E

V

E

D

D

P

A

D

A

V

V

G

E

A

D

L

L

L

L

A

A

T

E

V

V

-

S

Q

Q

P

P

G

-

G

-

S

K

R

R

I

D

I

F

S

S

D

E

E

E

E

E

I

I

V

I

Q

A

R

R

T

M

M

M

L

I

P

P

M

M

I

V

L

N

E

E

T

V

A

V

R

R

C

C

L

L

E

E

D

E

G

G

V

I

V

I

G

A

S

T

A

P

A

A

E

E

A

A

D

D

M

M

A

A

L

L

I

V

L

Y

G

G

I

L

G

G

F

F

P

P

R

P

H

F

L

H

G

G

G

G

A

A

L

F

H

R

Y

W

A

L

D

D

E

T

L

L

G

G

A

S

K

A

T

K

I

Y

M

L

E

D

W

M

C

A

D

Q

R

Q

Y

Y

A

Q

N

H

L

L

G

G

P

P

L

L

Y

Y

H

E

P

V

T

P

P

E

R

G

M

L

R

R

R

N

L

K

A

A

G116 (= G116) mutation to F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
G322 (= G320) mutation to A: 10-fold increase in KM for NADH.
H450 (= H448) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.

6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
49% identity, 98% coverage: 1:705/721 of query aligns to 2:707/719 of 6tnmA

query
sites
6tnmA

M

L

F

Y

Q

K

G

G

N

D

T

T

L

L

Q

Y

L

L

H

D

A

W

L

L

G

E

D

D

G

G

L

I

A

A

E

E

L

L

R

V

F

F

D

D

R

A

A

P

G

G

E

-

S

S

V

V

N

N

K

K

L

L

D

D

R

T

L

A

A

T

L

V

D

A

E

S

L

L

N

G

R

E

A

A

L

I

C

G

L

V

V

L

R

E

E

Q

D

Q

R

S

T

D

V

L

T

K

G

G

L

L

L

L

I

L

T

R

S

S

G

N

K

K

D

A

S

A

F

F

I

I

V

V

G

G

A
|
A

D

D

I

I

F

T

E

E

F
|
F

T

L

D

S

L

L

F

F

G

L

M

V

A

P

E

E

D

E

E

Q

I

L

A

S

I

Q

H

W

N

L

A

H

G

F

Q

A

S

N

A

S

V

V

I

F

T

N

A

R

L

L

A

E

D

D

L

L

P

P

V

V

P

P

T

T

V

I

V

A

A

A

V

V

N

N

G

G

L

Y

A

A

L

L

G

G

G
|
G

G

G

L

C

E
|
E

L

C

A

V

L

L

A

A

A

T

D

D

V

Y

R

R

M

L

L

A

S

T

D

P

S

D

A

L

R

R

I

I

G

G

L

L

P
|
P

E
|
E

V

T

G

K

L

L

G

G

I

I

F

M

P

P

G
|
G

F

F

G

G

G

G

T

S

V

V

R

R

L

M

P

P

R

R

L

M

I

L

G

G

V

A

E

D

A

S

A

A

L

L

Q

E

W

I

I

I

M

A

S

A

G

G

T

K

P

D

R

V

K

G

A

A

D

D

D

Q

A

A

L

L

R

K

A

I

G

G

A

L

V

V

D

D

A

G

V

V

V

V

P

K

P

A

P

E

E

K

L

L

R

V

E

E

A

G

A

A

L

K

E

A

R

V

L

L

-

R

H

Q

G

A

L

I

V

N

A

G

S

D

P

L

D

D

W

W

S

K

E

A

R

K

R

R

K

Q

E

P

A

K

L

L

S

E

P

P

V

L

V

K

R

L

A

S

G

K

E

I

D

E

L

A

A

T

P

M

I

S

F

F

A

T

A

I

A

A

K

K

A

G

K

M

A

V

R

A

K

Q

T

T

-

A

G

G

P

K

H

H

L

Y

P

P

A

A

S

P

L

I

A

T

V

A

V

V

E

K

L

T

I

I

E

E

S

A

A

A

A

A

G

R

V

F

D

G

R

R

D

E

A

E

A

A

L

L

A
x
N

L

L

E

E

A

N

R

K

A

S

F

F

A

V

H

P

I

L

A

A

R

H

T

T

P

N

A

E

A

A

R

R

A

A

L

L

V

V

A

G

V

I

F

F

I

L

N

N

D

D

Q

Q

A

Y

V

V

K

K

K

G

T

K

T

A

K

K

Q

K

Q

L

T

T

R

K

A

D

A

V

R

E

P

T

V

P

S

K

R

Q

A

A

A

A

V

V

L

L

G

G

A

A

G

G

I

I

M

M

G

G

G

G

G

G

I

I

A

A

Y

Y

Q

Q

S

S

A

A

V

W

C

K

G

G

I

V

P

P

V

V

I

V

M

M

K

K

D
|
D

I
|
I

A

N

Q

D

S

K

A

S

L

L

D

T

L

L

G

G

M

M

T

T

Q

E

A

A

G

A

A

K

L

L

L

L

A

N

K

K

R

Q

V

L

E

E

T

R

G

G

A

K

L

I

S

D

S

G

A

L

K

K

A

L

D

A

G

G

I

V

L

I

A

S

S

T

I

I

R

H

P

P

G

T

L

L

T

D

Y

Y

D

A

G

G

I

F

A

D

E

R

A

V

D

D

V

I

I

V

V

V

E

E

A

A

V
|
V

V
|
V

E

E

N

N

I

P

A

K

I
x
V

K

K

R

K

A

A

V

V

L

L

A

A

E

E

L

T

E

E

E

Q

H

K

I

V

R

R

P

Q

D

D

A

T

V

V

V

L

A

A

T

S

N

N

T

T

S
|
S

S

T

L

I

L

P

V

I

A

S

D

E

L

L

A

A

R

N

D

A

L

L

E

E

W

R

P

P

E

E

N

N

F

F

V

C

G

G

M

M

H
|
H

F

F

F

F

N

N

P

P

V

V

H

H

R

R

M

M

P

P

L

L

V

V

E
|
E

V

I

I

I

Q

R

G

G

P

E

H

K

T

S

S

S

P

D

E

E

A

T

V

I

A

A

T

K

I

V

V

V

G

A

Y

W

A

A

S

S

A

K

M

M

G

G

K

K

T

T

P

P

V

I

V

V

V

V

R

N

D

D

C

C

P

P

G

G

F

F

L

F

V

V

N
|
N

R

R

I

V

L

L

A

F

A

P

N

Y

V

F

L

A

G

G

F

F

L

S

R

Q

L

L

I

L

H

R

D

D

G

G

A

A

D

D

F

F

L

R

R

K

I

I

D

D

A

K

A

V

M

M

E

E

G

K

-

Q

F

F

G

G

W

W

P

P

M

M

G

G

P

P

A

A

W

Y

L

L

Q

L

D

D

V

V

I

V

G

G

M

I

D

D

T

T

S

A

R

H

H

H

V

A

I

Q

E

A

V

V

I

M

A

A

A

A

G

G

Y

F

P

P

E

Q

R

R

M

M

R

Q

V

K

E

D

F

Y

A

R

T

D

A

A

L

I

D

D

V

A

M

L

V

F

E

D

S

A

K

N

R

R

F

F

G

G

Q

Q

K
|
K

S

N

G

G

A

L

G

G

F

F

Y

W

R

R

Y

Y

E

K

A

E

D

D

A

S

A

K

G

G

H

K

Q

P

R

K

K

K

V

E

V

E

D

D

P

A

D

A

V

V

G

E

A

D

L

L

L

L

A

A

T

E

V

V

-

S

Q

Q

P

P

G

-

G

-

S

K

R

R

I

D

I

F

S

S

D

E

E

E

E

E

I

I

V

I

Q

A

R

R

T

M

M

M

L

I

P

P

M

M

I

V

L

N

E

E

T

V

A

V

R

R

C

C

L

L

E

E

D

E

G

G

V

I

V

I

G

A

S

T

A

P

A

A

E

E

A

A

D

D

M

M

A

A

L

L

I

V

L

Y

G

G

I

L

G

G

F

F

P

P

R

P

H

F

L

H

G

G

G

G

A

A

L

F

H

R

Y

W

A

L

D

D

E

T

L

L

G

G

A

S

K

A

T

K

I

Y

M

L

E

D

W

M

C

A

D

Q

R

Q

Y

Y

A

Q

N

H

L

L

G

G

P

P

L

L

Y

Y

H

E

P

V

T

P

P

E

R

G

M

L

R

R

R

N

L

K

A

A

active site: A68 (= A68), F73 (= F73), G116 (= G116), E119 (= E119), P138 (= P138), E139 (= E139), G147 (= G147), N271 (≠ A269), S429 (= S427), H450 (= H448), E462 (= E460), N500 (= N498)
binding adenosine-5'-triphosphate: D343 (= D341), I344 (= I342), V400 (= V398), V401 (= V399), V406 (≠ I404), K584 (= K581)

6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
36% identity, 97% coverage: 14:711/721 of query aligns to 11:702/707 of 6yswA

query
sites
6yswA

D

D

G

N

L

I

A

A

E

V

L

I

R

T

F

I

D

D

R

V

A

P

G

G

E
|
E

S

K

V
x
M

N

N

K

T

L

L

D

K

R

A

L

E

A

F

L

A

D

S

E

Q

L

V

N

R

R

A

A

I

L

I

C

K

L

Q

V

L

R

R

E

E

D

N

R

K

T

E

V

L

T

R

G

G

L

V

L

V

I

F

T

V

S

S

G

A

K

K

-

P

D

D

S

N

F

F

I

I

V

A

G

G

A
|
A

D
|
D

I
|
I

F

N

E

M

F
x
I

T

G

D

N

L

C

F

K

G

T

M

A

A

Q

E

E

D

A

E

E

I

-

A

A

I

L

H
x
A

N

R

A

Q

G

G

Q
|
Q

S

Q

A

-

V

L

I

M

T

A

A

E

L

I

A

H

D

A

L

L

P

P

V

I

P

Q

T

V

V

I

V

A

A

A

V

I

N

H

G

G

L

A

A

C

L

L

G

G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

A

A

C

D

H

V

G

R

R

M

V

L

C

S

T

D

D

S

D

A

P

R

K

-

T

-

V

I

L

G

G

L

L

P
|
P

E
|
E

V

V

G

Q

L
|
L

G

G

I

L

F

L

P

P

G
|
G

F

S

G

G

G

G

T

T

V

Q

R

R

L

L

P

P

R

R

L

L

I

I

G

G

V

V

E

S

A

T

A

A

L

L

Q

E

W

M

I

I

M

L

S

T

G

G

T

K

P

Q

R

L

K

R

A

A

D

K

D

Q

A

A

L

L

R

K

A

L

G

G

A

L

V

V

D

D

A

D

V

V

V

V

P

P

P

H

P

S

E

I

L

L

R

L

E

E

A

A

A

A

L

V

E

E

R

-

L

-

H

-

G

-

L

-

V

L

A

A

S

K

P

K

D

E

W

R

S

P

E

S

R

S

R

R

K

P

E

L

A

P

L

V

S

R

P

E

V

R

V

I

R

L

A

A

G

G

E

P

-

L

D

G

L

R

A

A

P

L

I

L

F

F

A

K

-

M

A

V

A

G

K

K

A

K

K

T

A

E

R

H

K

K

T

T

G

Q

P

G

H

N

L

Y

P

P

A

A

S

T

L

E

A

R

V

I

V

L

E

E

L

V

I

V

E

E

S

T

A

G

A

L

G

A

V

Q

D

G

R

T

D

S

A

S

A

G

L

Y

A
x
D

L

A

E

E

A

A

R

R

A

A

F

F

A

G

H

E

I

L

A

A

R

M

T

T

P

P

A

Q

A

S

R

Q

A

A

L

L

V

R

A

S

V

I

F

F

I

F

N

A

D

S

Q
x
T

A

D

V

V

K
|
K

K

K

T

D

T

P

K

G

Q

S

Q

D

T

A

R

P

A

P

A

A

R

-

P

P

V

L

S

N

R

S

A

V

A

G

V

I

L

L

G

G

A

G

G

G

I

L

M

M

G

G

G

G

G

G

I

I

A

A

Y

Y

Q

V

S

T

A

A

V

C

-

K

C

A

G

G

I

I

P

P

V

V

I

R

M

I

K

K

D

D

I

I

A

N

Q

P

S

Q

A

G

L

I

D

N

L

H

G

A

M

L

T

K

Q

Y

A

S

G

W

A

D

L

Q

L

L

A

E

K

G

R

K

V

V

E

R

T

R

G

R

A

H

L

L

S

K

S

A

A

S

K

E

A

R

D

D

G

K

I

Q

L

L

A

A

S

L

I

I

R

S

P

G

G

T

L

T

T

D

Y

Y

D

R

G

G

I

F

A

A

E

H

A

R

D

D

V

L

I

I

V

I

E

E

A

A

V

V

V

F

E

E

N

N

I

L

A

E

I

L

K

K

R

Q

A

Q

V

M

L

V

A

A

E

E

L

V

E

E

E

Q

H

N

I

C

R

A

P

A

D

H

A

T

V

I

V

F

A

A

T

S

N

N

T

T

S
|
S

S

S

L

L

L

P

V

I

A

G

D

D

L

I

A

A

R

A

D

H

L

A

E

T

W

R

P

P

E

E

N

Q

F

V

V

I

G

G

M

L

H
|
H

F

F

F

F

N

S

P

P

V

V

H

E

R

K

M

M

P

P

L

L

V

V

E
|
E

V

I

I

I

Q

P

G

H

P

A

H

G

T

T

S

S

P

A

E

Q

A

T

V

I

A

A

T

T

I

T

V

V

G

K

Y

L

A

A

S

K

A

K

M

Q

G

G

K

K

T

T

P

P

V

I

V

V

V

V

R

R

D

D

C

K

P

A

G

G

F

F

L

Y

V

V

N
|
N

R

R

I

I

L

L

A

A

A

P

N

Y

V

I

L

N

G

E

F

A

L

I

R

R

L

M

I

L

H

T

D

Q

G

G

A

E

D

R

F

V

L

E

R

H

I

I

D

D

A

A

A

A

M

L

E

V

G

K

F

F

G

G

W

F

P

P

M

V

G

G

P

P

A

I

W

Q

L

L

Q

L

D

D

V

E

I

V

G

G

M

I

D

D

T

T

S

G

R

T

H

K

V

I

I

I

E

P

V

V

I

L

A

E

A

A

G

A

Y

Y

P

G

E

E

R

R

M

F

R

S

V

A

E

P

F

-

A

A

T

N

A

V

L

V

D

S

V

S

M

I

V

L

E

N

S

D

K

D

R

R

F

K

G

G

Q

R

K

K

S

N

G

G

A

R

G

G

F

F

Y

Y

R

L

Y

Y

E

-

A

G

D

Q

A

K

A

G

G

R

H

K

Q

S

R

K

K

K

V

Q

V

V

D

D

P

P

D

A

V

I

G

Y

A

P

L

L

L

I

A

G

T

T

V

-

Q

-

P

-

G

Q

G

G

S

Q

R

G

I

R

I

I

S

S

D

A

E

P

E

Q

I

V

V

A

Q

E

R

R

T

C

M

V

L

M

P

L

M

M

I

L

L

N

E

E

T

A

A

V

R

R

C

C

L

V

E

D

D

E

G

Q

V

V

V

I

G

R

S

S

A

V

A

R

E

D

A

G

D

D

M

I

A

G

L

A

I

V

L

F

G

G

I

I

G

G

F

F

P

P

R

P

H

F

L

L

G

G

G

G

A

P

L

F

H

R

Y

Y

A

I

D

D

E

S

L

L

G

G

A

A

K

G

T

E

I

V

M

V

E

A

W

I

C

M

D

Q

R

R

Y

L

A

A

-

T

N

Q

L

Y

G

G

P

S

L

R

Y

F

H

T

P

P

T

C

P

E

R

R

M

L

R

V

R

E

L

M

A

G

V

A

D

R

G

G

A

E

G

S

W

F

active site: A66 (= A68), I71 (≠ F73), A84 (≠ H87), Q88 (= Q91), G112 (= G116), E115 (= E119), P136 (= P138), E137 (= E139), G145 (= G147), D264 (≠ A269), S422 (= S427), H443 (= H448), E455 (= E460), N493 (= N498)
binding coenzyme a: E23 (= E26), M25 (≠ V28), A66 (= A68), D67 (= D69), I68 (= I70), P136 (= P138), E137 (= E139), L140 (= L142), T290 (≠ Q295), K293 (= K298)

P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
32% identity, 97% coverage: 16:713/721 of query aligns to 49:758/763 of P40939

query
sites
P40939

L

V

A

A

E

V

L

V

R

R

F

I

D

N

R

S

A

P

G

N

E

S

S

K

V

V

N

N

K

T

L

L

D

S

R

K

L

E

A

L

L

H

D

S

E

E

L

F

N

S

R

E

A

V

L

M

C

N

L

E

V

I

-

W

R

A

E

S

D

D

R

Q

T

I

V

R

T

S

G

A

L

V

L

L

I

I

T

S

S

S

G

K

K

P

D

G

S

C

F

F

I

I

V

A

G

G

A

A

D

D

I

I

F

-

E

-

F

-

T

-

D

N

L

M

F

L

G

A

M

A

A

C

E

K

D

T

E

L

I

Q

A

E

I

V

H

T

N

Q

A

L

G

S

Q

Q

S

E

A

A

-

Q

-

R

V

I

I

V

T

E

A

K

L

L

A

E

D

K

L

S

P

T

V

K

P

P

T

I

V

V

V

A

A

A

V

I

N

N

G

G

L

S

A

C

L

L

G

G

G

G

G

G

L

L

E

E

L

V

A

A

L

I

A

S

A

C

D

Q

V

Y

R

R

M

I

L

A

S

T

D

K

S

D

A

R

R

K

-

T

-

V

I

L

G

G

L

T

P

P

E

E

V

V

G

L

L

L

G

G

I

A

F

L

P

P

G

G

F

A

G

G

G

G

T

T

V

Q

R

R

L

L

P

P

R

K

L

M

I

V

G

G

V

V

E

P

A

A

A

A

L

L

Q

D

W

M

I

M

M

L

S

T

G

G

T

R

P

S

R

I

K

R

A

A

D

D

D

R

A

A

L

K

R

K

A

M

G

G

A

L

V

V

D

D

A

Q

V

L

V

V

-

E

-

P

-

L

-

G

-

P

-

G

-

L

P

K

P

P

P

P

E

E

-

E

-

R

-

T

-

I

-

E

-

Y

L

L

R

E

E

E

A

V

A

A

L

I

E

T

R

F

L

A

H

K

G

G

L

L

V

-

A

A

S

D

P

K

D

K

W

I

S

S

E

P

R

K

R

R

K

D

E

K

A

G

L

L

S

V

P

E

V

K

V

L

R

T

A

A

G

Y

E

A

D

M

L

T

A

I

P

P

-

F

-

V

-

R

-

Q

-

Q

I
x
V

F

Y

A

K

A

K

A

V

K

E

A

E

K

K

A

V

R

R

K

K

T

Q

G

T

P

K

H

G

L

L

-

Y

P

P

A

A

S

P

L

L

A

K

V

I

V
x
I

E

D

L

V

I

V

E

K

S

T

A

G

A

I

G

E

V

Q

D

G

R

S

D

D

A

A

A

G

L

Y

A

L

L

C

E

E

A

S

R

Q

A

K

F

F

A

G

H

E

I

L

A

V

R

M

T

T

P

K

A

E

A

S

R

K

A

A

L

L

V

M

A

G

V
x
L

F

Y

I

H

N

G

D

Q

Q

V

A

L

V

C

K

K

K

K

T

N

T

-

K

-

Q

K

Q

F

T

G

R

A

A

P

A

Q

R

K

P

D

V

V

S

K

R

H

A

L

A

A

V

I

L

L

G

G

A

A

G

G

I

L

M

M

G

G

G

A

G

G

I

I

A

A

Y

Q

Q

V

S

S

A

V

V

D

C

K

G

G

I

L

P

K

V

T

I

I

M

L

K

K

D

D

I

A

A

T

Q

L

S

T

A

A

L

L

D

D

L

R

G

G

M

Q

T

Q

Q

Q

A

V

G

F

A

K

L

G

L

L

A

N

K

D

R

K

V

V

E

K

T

K

G

K

A

A

L

L

S

T

S

S

A

F

K

E

A

R

D

D

G

S

I

I

L

F

A

S

S

N

I

L

R

T

P

G

G

Q

L

L

T

D

Y

Y

D

Q

G

G

I

F

A

E

E

K

A

A

D

D

V

M

I

V

V

I

E

E

A

A

V

V

V

F

E

E

N

D

I

L

A

S

I

L

K

K

R

H

A

R

V

V

L

L

A

K

E

E

L

V

E

E

E

A

H

V

I

I

R

P

P

D

D

H

A

C

V

I

V

F

A

A

T

S

N

N

T

T

S

S

S

A

L

L

L

P

V

I

A

S

D

E

L

I

A

A

R

A

D

V

L

S

E

K

W

R

P

P

E

E

N

K

F

V

V

I

G

G

M

M

H

H

F

Y

F

F

N

S

P

P

V

V

H

D

R

K

M

M

P

Q

L

L

V

L

E
|
E

V

I

I

I

Q

T

G

T

P

E

H

K

T

T

S

S

P

K

E

D

A

T

V

S

A

A

T

S

I

A

V

V

G

A

Y

V

A

G

S

L

A

K

M

Q

G

G

K

K

T

V

P

I

V

I

V

V

V

V

R

K

D

D

C

G

P

P

G

G

F

F

L

Y

V

T

N

T

R

R

I

C

L

L

A

A

A

P

N

M

V

M

L

S

G

E

F

V

L

I

R

R

L

I

I

L

H

Q

D

E

G

G

A

V

D

D

F

P

L

K

R

K

I

L

D

D

A

S

A

L

M

T

E

T

G

S

F

F

G

G

W

F

P

P

M

V

G

G

P

A

A

A

W

T

L

L

Q

V

D

D

V

E

I

V

G

G

M

V

D

D

T

V

S

A

R

K

H

H

V

V

I

A

E

E

V

D

I

L

A

G

A

K

G

V

Y

F

P

G

E

E

R

R

M

F

R

G

V

G

E

G

F

N

A

P

T

E

A

L

L

L

D

T

V

Q

M

M

V

V

E

S

S

K

K

G

R

F

F

L

G

G

Q

R

K

K

S

S

G

G

A

K

G

G

F

F

Y

Y

R

I

Y

Y

E

Q

A

-

D

-

A

-

A

E

G

G

H

V

Q

K

R

R

K

K

V

D

V

L

D

N

P

S

D

D

V

M

G

D

A

S

L

I

L

L

A

A

T

S

V

L

Q

K

-

L

P

P

G

P

G

K

S

S

R

E

I

V

I

S

S

S

D

D

E

E

E

D

I

I

V

Q

Q

F

R

R

T

L

M

V

L

T

P

R

M

F

I

V

L

N

E

E

T

A

A

V

R

M

C

C

L

L

E

Q

D

E

G

G

V

I

V

L

G

A

S

T

A

P

A

A

E

E

A

G

D

D

M

I

A

G

L

A

I

V

L

F

G

G

I

L

G

G

F

F

P

P

R

P

H

C

L

L

G

G

G

G

A

P

L

F

H

R

Y

F

A

V

D

D

E

L

L

Y

G

G

A

A

K

Q

T

K

I

I

M

V

E

D

W

R

C

L

D

K

R

K

Y

Y

-

E

A

A

N

A

L

Y

G

G

P

K

L

Q

Y

F

H

T

P

P

T

C

P

-

R

-

M

-

R

-

R

Q

L

L

A

L

V

A

D

D

G

H

A

A

G

N

W

S

A

P

N

N

V282 (≠ I231) to D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
I305 (≠ V253) to N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
L342 (≠ V290) to P: in LCHAD deficiency; dbSNP:rs137852772
E510 (= E460) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769

6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
34% identity, 97% coverage: 10:711/721 of query aligns to 4:690/692 of 6iunB

query
sites
6iunB

H

Y

A

Q

L

V

G

Q

D

D

G

G

L

V

A

A

E

V

L

I

R

T

F

L

D

D

R

N

A

P

G

-

E

-

S

P

V

V

N

N

K

G

L

L

-

G

-

H

-

S

D

T

R

R

L

L

A

G

L

I

D

V

E

E

-

G

L

M

N

T

R

R

A

A

L

L

C

-

L

-

V

-

R

-

E

D

D

D

R

A

T

A

V

V

T

K

G

A

L

I

L

V

I

I

T

T

S

G

G

A

K

G

D

K

S

A

F

F

I

S

V

G

G

G

A
|
A

D

D

I

I

F

R

E

E

F
|
F

T

N

D

T

L

P

F

K

G

A

M

M

A

Q

E
|
E

D

P

E

T

I

L

A

-

I

-

H

-

N

-

A

-

G

-

Q

-

S
x
H

A

S

V

V

I

I

T

R

A

V

L

L

A

E

D

G

L

S

P

S

V

K

P

P

T

V

V

V

V

A

A

A

V

V

N

H

G

S

L

V

A

A

L

M

G

G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

G

A

C

D

N

V

Y

R

R

M

V

L

A

S

S

D

K

S

G

A

A

R

Q

I

I

G

A

L

L

P

P

E
|
E

V

V

G

K

L

L

G

G

I

L

F

L

P

P

G
|
G

F

A

G

G

G

G

T

T

V

Q

R

R

L

L

P

P

R

R

L

V

I

I

G

G

V

L

E

E

A

A

A

A

L

A

Q

N

W

M

I

I

M

V

S

S

G

G

T

T

P

P

-

V

-

L

-

S

-

E

R

K

K

F

A

A

D

G

D

T

A

K

L

L

R

F

A

D

G

E

A

I

V

V

D

D

A

G

V

D

V

V

P

L

P

P

P

A

E

A

L

V

R

K

E

F

A

A

A

-

L

-

E

-

R

Q

L

N

H

V

G

G

L

A

V

A

A

T

S

G

P

P

D

H

W

P

S

K

E

V

R

R

R

D

K

L

E

K

A

V

L

R

S

H

P

E

V

N

V

P

R

E

A

G

G

Y

E

L

D

G

L

F

A

A

P

R

I

N

F

T

A

V

A

A

A

A

K

M

A

A

K

K

A

-

R

-

K

-

T

-

G

-

P

-

H

N

L

F

P

P

A

A

S

P

L

L

A

-

V

-

V

-

E

K

L

C

I

L

E

E

S

A

A

V

A

A

G

G

V

S

D

L

R

K

-

P

-

F

D

E

A

Q

A

G

L

L

A
x
K

L

Q

E

E

A

R

R

E

A

G

F

F

A

L

H

Y

I

L

A

V

R

T

T

T

P

P

A

E

A

S

R

R

A

A

L

L

V

R

A

H

V

A

F

F

I

F

N

G

D

E

Q

R

A

A

V

A

K

S

K

K

T

I

T

P

K

D

-

V

Q

P

Q

E

T

G

R

T

A

P

A

T

R

R

P

K

V

I

S

E

R

K

A

V

A

A

V

V

L

I

G

G

A

A

G
|
G

I
x
T

M
|
M

G

G

G

G

G

G

I

I

A

S

Y

M

Q

N

S

F

A

L

V

N

C

A

G

G

I

I

P

P

V

V

I

T

M

I

K

L

D
x
E

I
x
T

A

K

Q

Q

S

E

A

A

L

L

D

D

L

R

G

G

M

V

T

G

Q

I

A

I

G

R

A

K

L

N

L

Y

A

E

K

N

R

S

V

A

E

K

T

K

G

G

A

K

L

L

S

T

S

Q

A

E

K

K

A

V

D

E

G

Q

I

R

L

M

A

G

S

L

I

L

R

S

P

T

G

T

L

L

T

S

Y
|
Y

D

D

G

D

I

L

A

K

E

D

A

A

D

D

V

L

I

I

V

I

E

E

A
|
A

V
|
V

V

F

E
|
E

N

E

I

M

A

G

I
x
V

K

K

R

E

A

T

V
|
V

L

F

A

K

E

K

L

L

E

D

E

E

H

V

I

A

R

K

P

Q

D

G

A

A

V

I

V

L

A

A

T

S

N
|
N

T

T

S
|
S

S

T

L

L

L

D

V

V

A

N

D

K

L

I

A

A

R

S

D

F

L

T

E

K

W

R

P

P

E

Q

N

D

F

V

V

V

G

G

M

M

H
|
H

F

F

F

F

N

S

P

P

V

A

H

N

R

V

M

M

P

K

L

L

V

L

E
|
E

V

V

I

V

Q

R

G

G

P

E

H

K

T

T

S

G

P

K

E

D

A

V

V

L

A

A

T

T

I

V

V

M

G

Q

Y

V

A

G

S

K

A

K

M

I

G

K

K

K

T

T

P

A

V

V

V

V

V

S

R

G

D

V

C

C

P

D

G

G

F

F

L

I

V

G

N
|
N

R

R

I

M

L

I

A

E

A

Q

N

Y

V

S

L

R

G

Q

F

A

L

G

R

Y

L

L

I

L

H

D

D

E

G

G

A

A

D

L

F

P

L

E

R

Q

I

V

D

D

A

K

A

A

M

I

E

E

G

K

F

F

G

G

W

F

P

A

M

M

G

G

P

P

A

F

W

R

L

M

Q

G

D

D

V

L

I

A

G

G

M

N

D

D

T

I

S

G

R

W

H

A

V

I

I

R

E

K

V

R

I

R

A

A

A

V

G

D

Y

K

P

P

E

E

R

-

M

-

R

-

V

I

E

Q

F

Y

A

S

T

K

A

T

L

A

D

D

V

L

M

L

V

C

E

E

S

M

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

A

G

G

F

W

Y

Y

R

D

Y

Y

E

K

A

-

D

-

A

-

A

A

G

G

H

D

Q

R

R

K

K

P

V

Y

V

P

D

N

P

Q

D

Q

V

V

G

N

A

D

L

M

L

I

A

V

T

Q

V

H

Q

S

P

K

G

D

-

L

-

G

-

I

G

T

S

R

R

R

I

K

I

I

S

S

D

D

E

E

E

E

I

I

V

V

Q

E

R

R

T

L

M

V

L

F

P

A

M

L

I

V

L

N

E

E

T

G

A

A

R

R

C

I

L

L

E

E

D

E

G

G

V

I

V

A

G

S

S

K

A

A

A

S

E

D

A

I

D

D

M

M

A

V

L

Y

I

L

L

T

G

G

I

Y

G

G

F

F

P

P

R

L

H

F

L

R

G

G

G

G

A

P

L

M

H

L

Y

Y

A

A

D

D

E

Q

L

V

G

G

A

L

K

Y

T

N

I

V

M

A

E

L

W

S

C

M

D

K

R

R

Y

Y

A

A

N

K

-

G

-

Y

L

H

G

G

P

E

L

A

Y

W

H

Q

P

V

T

A

P

P

R

L

M

L

R

Q

R

K

L

L

A

A

V

D

D

E

G

G

A

K

G

G

W

F

active site: A60 (= A68), F65 (= F73), E73 (= E81), H77 (≠ S92), G101 (= G116), E104 (= E119), E124 (= E139), G132 (= G147), K248 (≠ A269), S407 (= S427), H428 (= H448), E440 (= E460), N478 (= N498)
binding nicotinamide-adenine-dinucleotide: G300 (= G320), T301 (≠ I321), M302 (= M322), E321 (≠ D341), T322 (≠ I342), Y365 (= Y385), A377 (= A397), V378 (= V398), E380 (= E400), V384 (≠ I404), V388 (= V408), N405 (= N425), S407 (= S427)

Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
31% identity, 96% coverage: 28:720/721 of query aligns to 21:721/723 of Q08426

query
sites
Q08426

V

V

N

N

K

A

L

I

D

S

R

T

L

T

A

L

L

L

D

R

E

D

L

I

N

K

R

E

A

G

L

L

C

Q

L

K

V

A

R
x
V

E
x
I

D

D

R

H

T

T

V

I

T

K

G

A

L

I

L

V

I

I

T

C

S

G

G

A

K

E

D

G

S

K

F

F

I

S

V

A

G

G

A

A

D

D

I

I

F

R

E

G

F

F

T

S

-

A

-

P

D

R

L

T

F

F

G

G

M

L

A
x
T

E

L

D

G

E

H

I

-

A

-

I

-

H

-

N

-

A

-

G

-

Q

-

S

-

A

-

V

V

I

V

T

D

A

E

L

I

A

Q

D

R

L

N

P

E

V

K

P

P

T

V

V

V

V

A

A

A

V

I

N

Q

G

G

L

M

A

A

L

F

G

G

G

G

G

G

L

L

E

E

L

L

A

A

L

L

A

G

A

C

D

H

V

Y

R

R

M

I

L

A

S

H

D

A

S

E

A

A

R

Q

I

V

G

G

L

L

P

P

E

E

V

V

G

T

L

L

G

G

I

L

F

L

P

P

G

G

F

A

G

R

G

G

T

T

V

Q

R

L

L

L

P

P

R

R

L

L

I

T

G

G

V

V

E

P

A

A

A

A

L

L

Q

D

W

L

I

I

M

T

S

S

G

G

T

R

P

R

R

I

K

L

A

A

D

D

D

E

A

A

L

L

R
x
K

A

L

G

G

A

I

V

L

D

D

A
x
K

V

V

V

V

P

N

P

S

P

D

E

P

L

V

R

E

E

E

A

A

A

I

L

-

E

-

R

R

L

F

H

A

G

Q

L

R

V

V

A

S

S

D

P

Q

D

P

W

L

S

E

E

S

R

R

R

R

K

-

E

-

A

L

L

C

S

N

P

K

V

P

V

I

R

Q

A

S

G

L

E

P

D

N

L

M

A

D

P

S

I

I

F

F

A

S

A

E

A

A

K

L

A

L

K

K

A

M

R

R

K

R

T

Q

G

H

P

P

H

G

L

C

P

L

A

A

S

Q

L

E

A

A

V

C

V

V

E

R

L

A

I

V

E

Q

S

A

A

A

A

V

G

Q

V

Y

D

P

R

Y

D

E

A

V

A

G

L

I

A

K

L

K

E

E

A

E

R

E

A

L

F

F

A

L

H

Y

I

L

A

L

R

Q

T

S

P

G

A

Q

A

A

R

R

A

A

L

L

V

Q

A

Y

V

A

F

F

I

F

N
x
A

D

E

Q

R

A

K

V

A

K

N

K

K

T

W

T

S

-

T

-

P

-

S

-

G

-

A

K

S

Q

W

Q

K

T

T

R

A

A

S

A

A

R

R

P

P

V

V

S

S

R

S

A

V

A

G

V

V

L

V

G

G

A

L

G

G

I

T

M

M

G

G

G

R

G

G

I

I

A

V

Y

I

Q

S

S

F

A

A

V

R

C

A

G

R

I

I

P

P

V

V

I

I

M
x
A

K

V

D

D

I

S

A

D

Q

K

S

N

A

Q

L

L

D

-

L

-

G

-

M

-

T

A

Q

T

A

A

G

N

A

K

L

M

L

I

A

T

K

S

R

V

V

L

E

E

T
x
K

G

E

A

A

L

S

S

K

S

M

A

Q

K

Q

A

S

D

G

G

H

I

P

L

W

A

S

S

G

I

P

R

K

P

P

G

R

L

L

T

T

-

S

-

S

Y

V

D

K

G

E

I

L

A

G

E

G

A

V

D

D

V

L

I

V

V

I

E

E

A

A

V

V

V

F

E

E

N

E

I

M

A

S

I

L

K

K

R

K

A

Q

V

V

L

F

A

A

E

E

L

L

E

S

E

A

H

V

I

C

R

K

P

P

D

E

A

A

V

F

V

L

A

C

T

T

N

N

T

T

S

S

S

A

L

L

L

D

V

V

A

D

D

E

L

I

A

A

R

S

D

S

L

T

E

D

W

R

P

P

E

H

N

L

F

V

V

I

G

G

M

T

H

H

F

F

F

F

N

S

P

P

V

A

H

H

R

V

M

M

P

K

L

L

V

L

E

E

V

V

I

I

Q

P

G

S

P

Q

H

Y

T

S

S

S

P

P

E

T

A

T

V

I

A

A

T

T

I

V

V

M

G

N

Y

L

A

S

S

K

A

K

M

I

G

K

K

K

T

I

P

G

V

V

V

V

V

V

R

G

D

N

C

C

P

F

G

G

F

F

L

V

V

G

N

N

R

R

I

M

L

L

A

N

A

P

N

Y

V

Y

L

N

G

Q

F

A

L

Y

R

F

L

L

I

L

H

E

D

E

G

G

A

S

D

K

F

P

L

E

R

E

I

V

D

D

A

Q

A

V

M

L

E

E

G

E

F

F

G

G

W

F

P

K

M

M

G

G

P

P

A

F

W

R

L

V

Q

S

D

D

V

L

I

A

G

G

M

L

D

D

-

V

-

G

-

W

T

K

S

S

R

R

H

K

-

G

-

Q

-

G

-

L

V

T

I

G

E

P

V

T

I

L

A

L

A

P

G

G

Y

T

P

P

E

A

R

R

M

K

R

R

-

G

-

N

V

R

E

R

F

Y

A

C

T

P

A

I

L

P

D

D

V

V

M

L

V

C

E

E

S

L

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

K

G

G

F

W

Y

Y

R

Q

Y

Y

E

D

A
x
K

D

P

A

L

A

G

G

R

H

I

Q

H

R

K

K

P

V

-

V

-

D

D

P

P

D

W

V

L

G

S

A
x
K

L

F

L

L

A

S

T

R

V

Y

Q

R

P

K

G
x
T

G

H

S

H

-

I

-

E

-

P

R

R

I

T

I

I

S

S

D

Q

E

D

E

E

I

I

V

L

Q

E

R

R

T

C

M

L

L

Y

P

S

M

L

I

I

L

N

E

E

T

A

A

F

R

R

C

I

L

L

E

G

D

E

G

G

V

I

V

A

G

A

S

S

A

P

A

E

E

H

A

I

D

D

M

V

A

V

L

Y

I

L

L

H

G

G

I

Y

G

G

F

W

P

P

R

R

H

H

L

K

G

G

G

G

A

P

L

M

H

F

Y

Y

A

A

D

S

E

T

L

V

G

G

A

L

K

P

T

T

I

V

M

L

E

E

W

K

C

L

D

Q

R

K

Y

Y

A

Y

N

R

L

Q

G

N

-

P

-

D

-

I

P

P

L

Q

Y

L

H

E

P

P

T

S

P

D

R

Y

M

L

R

K

R

K

L

L

A

A

V

S

D

Q

G

G

-

N

-

P

-

P

-

L

A

K

G

E

W

W

A

Q

N

S

L

L

V

A

Q

G

R

S

P

P

G

S

A

S

V40 (≠ R47) to G: in dbSNP:rs1062551
I41 (≠ E48) to R: in dbSNP:rs1062552
T75 (≠ A80) to I: in dbSNP:rs1062553
K165 (≠ R180) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
K171 (≠ A186) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
A274 (≠ N293) to T: in dbSNP:rs2302819
A325 (≠ M339) to G: in dbSNP:rs1062555
K346 (≠ T364) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
K584 (≠ A591) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
K598 (≠ A607) to T: in dbSNP:rs1042437
T606 (≠ G615) to P: in dbSNP:rs1042438

Sites not aligning to the query:

3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646

3zw9A Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with (2s,3s)-3-hydroxy-2-methylbutanoyl-coa (see paper)
33% identity, 95% coverage: 28:709/721 of query aligns to 24:708/723 of 3zw9A

query
sites
3zw9A

V
|
V

N

N

K

A

L

V

D

S

R

P

L

T

A

V

L

I

D

R

E

E

L

V

N

R

R

N

A

G

L

L

C

Q

L

K

V

A

R

G

E

S

D

D

R

H

T

T

V

V

T

K

G

A

L

I

L

V

I

I

T

C

S

G

G

A

K

N

D

G

S

N

F

F

I

C

V
x
A

G
|
G

A
|
A

D

D

I
|
I

F

H

E

G

F
|
F

T

S

D

A

L

F

-

T

-

P

-

G

-

L

-

A

F

L

G
|
G

M

S

A

L

E

V

D

D

E

E

I

I

A

Q

I

R

H

Y

N

Q

A

K

G

-

Q

-

S

-

A

-

V

-

I

-

T

-

A

-

L

-

A

-

D

-

L

-

P

-

V

-

P

P

T

V

V

L

V

A

A

A

V

I

N

Q

G

G

L

V

A

A

L

L

G
|
G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

G

A

C

D

H

V

Y

R

R

M

I

L

A

S

N

D

A

S

K

A

A

R

R

I

V

G

G

L

L

P
|
P

E
|
E

V

V

G

T

L

L

G

G

I

I

F

L

P
|
P

G
|
G

F

A

G

R

G

G

T

T

V

Q

R

L

L

L

P

P

R

R

L

V

I

V

G

G

V

V

E

P

A

V

A

A

L

L

Q

D

W

L

I

I

M

T

S

S

G

G

T

K

P
x
Y

R

L

K

S

A

A

D

D

D

E

A

A

L

L

R

R

A

L

G

G

A

I

V

L

D

D

A

A

V

V

V

V

P

K

P

S

P

D

E

P

L

V

R

E

E

E

A

A

A

-

L

I

E

K

R

F

L

A

H

Q

G

K

L

I

V

I

A

D

S

K

P

P

D

-

W

-

S

I

E

E

R

P

R

R

K

R

E

I

A

F

L

N

S

K

P

P

V

V

V

P

R

S

A

L

G

-

E

P

D

N

L

M

A

D

P

S

I

V

F

F

A

A

A

E

A

A

K

I

A

A

K

K

A

V

R

R

K

K

T

Q

G

Y

P

P

H

G

L

V

P

L

A

A

S

P

L

E

A

T

V

C

V

V

E

R

L

S

I

I

E

Q

S

A

A

S

A

V

G

K

V

H

D

P

R

Y

D

E

A

V

A

G

L

I

A
x
K

L

E

E

E

A

E

R

K

A

L

F

F

A

M

H

Y

I

L

A

R

R

A

T

S

P

G

A

Q

A

A

R

K

A

A

L

L

V

Q

A

Y

V

A

F

F

I

F

N

A

D

E

Q

K

A

S

V

A

K

N

K

K

T

W

T

S

-

T

-

P

-

S

-

G

-

A

K

S

Q

W

Q

K

T

T

R

A

A

S

A

A

R

Q

P

P

V

V

S

S

R

S

A

V

A

G

V

V

L
|
L

G
|
G

A

L

G
|
G

I
x
T

M
|
M

G

G

G

R

G

G

I

I

A

A

Y

I

Q

S

S

F

A

A

V

R

C

V

G

G

I

I

P

S

V

V

I

V

M

A

K

V

D
x
E

I

S

A

D

Q

P

S

K

A
x
Q

L

L

D

D

L

A

G

-

M

-

T

-

Q

-

A

-

G

-

A

-

L

-

L

-

A

A

K

K

R

K

V

I

E

I

T

T

G

F

A

T

L

L

-

E

-

K

-

E

-

A

S

S

S

R

A

A

K

H

A

Q

D

N

G

G

I

-

L

Q

A

A

S

S

I

A

R

K

P

P

G

K

L

L

T

R

Y

F

D

S

G

S

-

S

-

T

-

K

-

E

I

L

A

S

E

T

A

V

D

D

V

L

I

V

V

V

E

E

A
|
A

V
|
V

V
x
F

E
|
E

N

D

I

M

A

N

I

L

K

K

R

K

A

K

V

V

L

F

A

A

E

E

L

L

E

S

E

A

H

L

I

C

R

K

P

P

D

G

A

A

V

F

V

L

A

C

T

T

N
|
N

T

T

S
|
S

S

A

L

L

L

N

V

V

A

D

D

D

L

I

A

A

R

S

D

S

L

T

E

D

W

R

P

P

E

Q

N

L

F

V

V

I

G

G

M

T

H
|
H

F

F

F

F

N

S

P

P

V

A

H

H

R

V

M

M

P

R

L

L

V

L

E
|
E

V

V

I

I

Q

P

G

S

P

R

H

Y

T

S

S

S

P

P

E

T

A

T

V

I

A

A

T

T

I

V

V

M

G

S

Y

L

A

S

S

K

A

K

M

I

G

G

K

K

T

I

P

G

V

V

V

V

V

V

R

G

D

N

C

C

P

Y

G

G

F

F

L

V

V

G

N
|
N

R

R

I

M

L

L

A

A

A

P

N

Y

V

Y

L

N

G

Q

F

G

L

F

R

F

L

L

I

L

H

E

D

E

G

G

A

S

D

K

F

P

L

E

R

D

I

V

D

D

A

G

A

V

M

L

E

E

G

E

F

F

G

G

W

F

P

K

M

M

G

G

P

P

A

F

W

R

L

V

Q

S

D

D

V

L

I

A

G

G

M

L

D

D

T

V

S

G

R

W

H

K

V

I

I

R

E

K

-

G

-

Q

-

G

-

L

-

T

-

G

-

P

V

S

I

L

A

P

A

P

G

G

Y

T

P

P

E

V

R

R

M

K

R

R

-

G

-

N

V

S

E

R

F

Y

A

S

T

P

A

L

L

G

D

D

V

M

M

L

V

C

E

E

S

A

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

K

G

G

F

W

Y

Y

R

Q

Y

Y

E

D

A

K

D

P

A

L

A

G

G

R

H

I

Q

H

R

K

K

P

V

-

V

-

D

D

P

P

D

W

V

L

G

S

A

T

L

F

L

L

A

S

T

Q

-

Y

-

R

-

E

V

V

Q

H

P

H

G

I

G

E

S

Q

R

R

I

T

I

I

S

S

D

K

E

E

E

E

I

I

V

L

Q

E

R

R

T

C

M

L

L

Y

P

S

M

L

I

I

L

N

E

E

T

A

A

F

R

R

C

I

L

L

E

E

D

E

G

G

V

M

V

A

G

A

S

R

A

P

A

E

E

H

A

I

D

D

M

V

A

I

L

Y

I

L

L

H

G

G

I

Y

G

G

F

W

P

P

R

R

H

H

L

K

G

G

G

G

A

P

L

M

H

F

Y

Y

A

A

D

A

E

S

L

V

G

G

A

L

K

P

T

T

I

V

M

L

E

E

W

K

C

L

D

Q

R

K

Y

Y

A

Y

N

R

L

Q

G

N

-

P

-

D

-

I

P

P

L

Q

Y

L

H

E

P

P

T

S

P

D

R

Y

M

L

R

R

R

R

L

L

A

V

V

A

D

Q

G

G

A

S

active site: A64 (= A68), F69 (= F73), G79 (= G78), G103 (= G116), E106 (= E119), P125 (= P138), E126 (= E139), P133 (= P146), G134 (= G147), K252 (≠ A269), S413 (= S427), H434 (= H448), E446 (= E460), N484 (= N498)
binding nicotinamide-adenine-dinucleotide: L305 (= L317), G306 (= G318), G308 (= G320), T309 (≠ I321), M310 (= M322), E329 (≠ D341), Q334 (≠ A346), A383 (= A397), V384 (= V398), F385 (≠ V399), E386 (= E400), N411 (= N425), S413 (= S427), H434 (= H448)
binding (2s,3s)-3-hydroxy-2-methylbutanoyl-coa: V24 (= V28), A62 (≠ V66), G63 (= G67), A64 (= A68), I66 (= I70), G102 (= G115), G103 (= G116), E106 (= E119), E126 (= E139), P133 (= P146), Y159 (≠ P172)

3zwaA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-hexanoyl-coa (see paper)
33% identity, 95% coverage: 28:709/721 of query aligns to 27:711/727 of 3zwaA

query
sites
3zwaA

V
|
V

N

N

K

A

L

V

D

S

R

P

L

T

A

V

L

I

D

R

E

E

L

V

N

R

R

N

A

G

L

L

C

Q

L

K

V

A

R

G

E

S

D

D

R

H

T

T

V

V

T

K

G

A

L

I

L

V

I

I

T

C

S

G

G

A

K

N

D

G

S

N

F

F

I

C

V
x
A

G
|
G

A
|
A

D
|
D

I
|
I

F

H

E

G

F
|
F

T

S

D

A

L

F

-

T

-

P

-

G

-

L

-

A

F

L

G
|
G

M

S

A

L

E

V

D

D

E

E

I

I

A

Q

I

R

H

Y

N

Q

A

K

G

-

Q

-

S

-

A

-

V

-

I

-

T

-

A

-

L

-

A

-

D

-

L

-

P

-

V

-

P

P

T

V

V

L

V

A

A

A

V

I

N

Q

G

G

L

V

A

A

L
|
L

G

G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

G

A

C

D

H

V

Y

R

R

M

I

L

A

S

N

D

A

S

K

A

A

R
|
R

I

V

G

G

L

L

P
|
P

E
|
E

V

V

G

T

L
|
L

G

G

I

I

F

L

P
|
P

G
|
G

F

A

G

R

G

G

T

T

V

Q

R

L

L

L

P

P

R

R

L

V

I

V

G

G

V

V

E

P

A

V

A

A

L

L

Q

D

W

L

I

I

M

T

S

S

G

G

T

K

P
x
Y

R

L

K

S

A

A

D

D

D

E

A

A

L

L

R

R

A

L

G

G

A

I

V

L

D

D

A

A

V

V

V

V

P

K

P

S

P

D

E

P

L

V

R

E

E

E

A

A

A

-

L

I

E

K

R

F

L

A

H

Q

G

K

L

I

V

I

A

D

S

K

P

P

D

-

W

-

S

I

E

E

R

P

R

R

K

R

E

I

A

F

L

N

S

K

P

P

V

V

V

P

R

S

A

L

G

-

E

P

D

N

L

M

A

D

P

S

I

V

F

F

A

A

A

E

A

A

K

I

A

A

K

K

A

V

R

R

K

K

T

Q

G

Y

P

P

H

G

L

V

P

L

A

A

S

P

L

E

A

T

V

C

V

V

E

R

L

S

I

I

E

Q

S

A

A

S

A

V

G

K

V

H

D

P

R

Y

D

E

A

V

A

G

L

I

A
x
K

L

E

E

E

A

E

R

K

A

L

F

F

A

M

H

Y

I

L

A

R

R

A

T

S

P

G

A

Q

A

A

R

K

A

A

L

L

V

Q

A

Y

V

A

F

F

I

F

N

A

D

E

Q

K

A

S

V

A

K

N

K

K

T

W

T

S

-

T

-

P

-

S

-

G

-

A

K

S

Q

W

Q

K

T

T

R

A

A

S

A

A

R

Q

P

P

V

V

S

S

R

S

A

V

A

G

V

V

L

L

G

G

A

L

G

G

I

T

M

M

G

G

G

R

G

G

I

I

A

A

Y

I

Q

S

S

F

A

A

V

R

C

V

G

G

I

I

P

S

V

V

I

V

M

A

K

V

D

E

I

S

A

D

Q

P

S

K

A

Q

L

L

D

D

L

A

G

-

M

-

T

-

Q

-

A

-

G

-

A

-

L

-

L

-

A

A

K

K

R

K

V

I

E

I

T

T

G

F

A

T

L

L

-

E

-

K

-

E

-

A

S

S

S

R

A

A

K

H

A

Q

D

N

G

G

I

-

L

Q

A

A

S

S

I

A

R

K

P

P

G

K

L

L

T

R

Y

F

D

S

G

S

-

S

-

T

-

K

-

E

I

L

A

S

E

T

A

V

D

D

V

L

I

V

V

V

E

E

A

A

V

V

V

F

E

E

N

D

I

M

A

N

I

L

K

K

R

K

A

K

V

V

L

F

A

A

E

E

L

L

E

S

E

A

H

L

I

C

R

K

P

P

D

G

A

A

V

F

V

L

A

C

T

T

N

N

T

T

S
|
S

S

A

L

L

L

N

V

V

A

D

D

D

L

I

A

A

R

S

D

S

L

T

E

D

W

R

P

P

E

Q

N

L

F

V

V

I

G

G

M

T

H
|
H

F

F

F

F

N

S

P

P

V

A

H

H

R

V

M

M

P

R

L

L

V

L

E
|
E

V

V

I

I

Q

P

G

S

P

R

H

Y

T

S

S

S

P

P

E

T

A

T

V

I

A

A

T

T

I

V

V

M

G

S

Y

L

A

S

S

K

A

K

M

I

G

G

K

K

T

I

P

G

V

V

V

V

V

V

R

G

D

N

C

C

P

Y

G

G

F

F

L

V

V

G

N
|
N

R

R

I

M

L

L

A

A

A

P

N

Y

V

Y

L

N

G

Q

F

G

L

F

R

F

L

L

I

L

H

E

D

E

G

G

A

S

D

K

F

P

L

E

R

D

I

V

D

D

A

G

A

V

M

L

E

E

G

E

F

F

G

G

W

F

P

K

M

M

G

G

P

P

A

F

W

R

L

V

Q

S

D

D

V

L

I

A

G

G

M

L

D

D

T

V

S

G

R

W

H

K

V

I

I

R

E

K

-

G

-

Q

-

G

-

L

-

T

-

G

-

P

V

S

I

L

A

P

A

P

G

G

Y

T

P

P

E

V

R

R

M

K

R

R

-

G

-

N

V

S

E

R

F

Y

A

S

T

P

A

L

L

G

D

D

V

M

M

L

V

C

E

E

S

A

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

K

G

G

F

W

Y

Y

R

Q

Y

Y

E

D

A

K

D

P

A

L

A

G

G

R

H

I

Q

H

R

K

K

P

V

-

V

-

D

D

P

P

D

W

V

L

G

S

A

T

L

F

L

L

A

S

T

Q

-

Y

-

R

-

E

V

V

Q

H

P

H

G

I

G

E

S

Q

R

R

I

T

I

I

S

S

D

K

E

E

E

E

I

I

V

L

Q

E

R

R

T

C

M

L

L

Y

P

S

M

L

I

I

L

N

E

E

T

A

A

F

R

R

C

I

L

L

E

E

D

E

G

G

V

M

V

A

G

A

S

R

A

P

A

E

E

H

A

I

D

D

M

V

A

I

L

Y

I

L

L

H

G

G

I

Y

G

G

F

W

P

P

R

R

H

H

L

K

G

G

G

G

A

P

L

M

H

F

Y

Y

A

A

D

A

E

S

L

V

G

G

A

L

K

P

T

T

I

V

M

L

E

E

W

K

C

L

D

Q

R

K

Y

Y

A

Y

N

R

L

Q

G

N

-

P

-

D

-

I

P

P

L

Q

Y

L

H

E

P

P

T

S

P

D

R

Y

M

L

R

R

R

R

L

L

A

V

V

A

D

Q

G

G

A

S

active site: A67 (= A68), F72 (= F73), G82 (= G78), G106 (= G116), E109 (= E119), P128 (= P138), E129 (= E139), P136 (= P146), G137 (= G147), K255 (≠ A269), S416 (= S427), H437 (= H448), E449 (= E460), N487 (= N498)
binding (S)-3-Hydroxyhexanoyl-CoA: V27 (= V28), A65 (≠ V66), G66 (= G67), A67 (= A68), D68 (= D69), I69 (= I70), L104 (= L114), E109 (= E119), R124 (= R134), E129 (= E139), L132 (= L142), G137 (= G147), Y162 (≠ P172)

6z5oAAA Peroxisomal bifunctional enzyme (see paper)
32% identity, 95% coverage: 28:709/721 of query aligns to 27:704/716 of 6z5oAAA

query
sites
6z5oAAA

V
|
V

N

N

K

A

L

V

D

S

R

P

L

T

A

V

L

I

D

R

E

E

L

V

N

R

R

N

A

G

L

L

C

Q

L

K

V

A

R

G

E

S

D

D

R

H

T

T

V

V

T

K

G

A

L

I

L

V

I

I

T

C

S

G

G

A

K

N

D

G

S

N

F

F

I

C

V
x
A

G

G

A
|
A

D
|
D

I
|
I

F

H

E

G

F
|
F

T

S

D

A

L

F

-

T

-

P

-

G

-

L

-

A

F

L

G
|
G

M

S

A

L

E

V

D

D

E

E

I

I

A

Q

I

R

H

Y

N

Q

A

K

G

-

Q

-

S

-

A

-

V

-

I

-

T

-

A

-

L

-

A

-

D

-

L

-

P

-

V

-

P

P

T

V

V

L

V

A

A

A

V

I

N

Q

G

G

L

V

A

A

L

L

G

G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

G

A

C

D

H

V

Y

R

R

M

I

L

A

S

N

D

A

S

K

A

A

R

R

I

V

G

G

L

L

P
|
P

E
|
E

V

V

G

T

L

L

G

G

I

I

F

L

P
|
P

G
|
G

F

A

G

R

G

G

T

T

V

Q

R

L

L

L

P

P

R

R

L

V

I

V

G

G

V

V

E

P

A

V

A

A

L

L

Q

D

W

L

I

I

M

T

S

S

G

G

T

K

P
x
Y

R

L

K

S

A

A

D

D

D

E

A

A

L

L

R

R

A

L

G

G

A

I

V

L

D

D

A

A

V

V

V

V

P

K

P

S

P

D

E

P

L

V

R

E

E

E

A

A

A

-

L

I

E

K

R

F

L

A

H

Q

G

K

L

I

V

I

A

D

S

K

P

P

D

-

W

-

S

I

E

E

R

P

R

R

K

R

E

I

A

F

L

N

S

K

P

P

V

V

V

P

R

S

A

L

G

-

E

P

D

N

L

M

A

D

P

S

I

V

F

F

A

A

A

E

A

A

K

I

A

A

K

K

A

V

R

R

K

K

T

Q

G

Y

P

P

H

G

L

V

P

L

A

A

S

P

L

E

A

T

V

C

V

V

E

R

L

S

I

I

E

Q

S

A

A

S

A

V

G

K

V

H

D

P

R

Y

D

E

A

V

A

G

L

I

A
x
K

L

E

E

E

A

E

R

K

A

L

F
|
F

A

M

H

Y

I

L

A

R

R

A

T

S

P

G

A

Q

A

A

R

K

A

A

L

L

V

Q

A

Y

V

A

F
|
F

I

F

N

A

D

E

Q
x
K

A

S

V

A

K

N

K

K

T

W

T

S

-

T

-

P

-

S

-

G

-

A

K

S

Q

W

Q

K

T

T

R

A

A

S

A

A

R

Q

P

P

V

V

S

S

R

S

A

V

A

G

V

V

L

L

G
|
G

A

L

G
|
G

I
x
T

M
|
M

G

G

G

R

G

G

I

I

A

A

Y

I

Q

S

S

F

A

A

V

R

C

V

G

G

I

I

P

S

V

V

I

V

M

A

K

V

D
x
E

I
x
S

A

D

Q

P

S

K

A
x
Q

L

L

D

D

L

A

G

-

M

-

T

-

Q

-

A

-

G

-

A

-

L

-

L

-

A

A

K

K

R

K

V

I

E

I

T

T

G

F

A

T

L

L

S

E

S

K

A

E

K

A

A

S

D

R

G

A

I

H

L

Q

A

P

S

K

I

L

R

R

P

F

G

S

L

S

T

S

Y

T

D

K

G

E

I

L

A

S

E

T

A

V

D

D

V

L

I

V

V

V

E

E

A
|
A

V
|
V

V
x
F

E
|
E

N

D

I

M

A

N

I

L

K
|
K

R

K

A

K

V

V

L

F

A

A

E

E

L

L

E

S

E

A

H

L

I

C

R

K

P

P

D

G

A

A

V

F

V

L

A

C

T

T

N
|
N

T

T

S
|
S

S

A

L

L

L

N

V

V

A

D

D

D

L

I

A

A

R

S

D

S

L

T

E

D

W

R

P

P

E

Q

N

L

F

V

V

I

G

G

M

T

H
|
H

F

F

F

F

N

S

P

P

V

A

H

H

R

V

M

M

P

R

L

L

V

L

E
|
E

V

V

I

I

Q

P

G

S

P

R

H

Y

T

S

S

S

P

P

E

T

A

T

V

I

A

A

T

T

I

V

V

M

G

S

Y

L

A

S

S

K

A

K

M

I

G

G

K

K

T

I

P

G

V

V

V

V

V

V

R

G

D

N

C

C

P

Y

G

G

F

F

L

V

V

G

N
|
N

R

R

I

M

L

L

A

A

A

P

N

Y

V

Y

L

N

G

Q

F

G

L

F

R

F

L

L

I

L

H

E

D

E

G

G

A

S

D

K

F

P

L

E

R

D

I

V

D

D

A

G

A

V

M

L

E

E

G

E

F

F

G

G

W

F

P

K

M

M

G

G

P

P

A

F

W

R

L

V

Q

S

D

D

V

L

I

A

G

G

M

L

D

D

T

V

S

G

R

W

H

K

V

I

I

R

E

K

-

G

-

Q

-

G

-

L

-

T

-

G

-

P

V

S

I

L

A

P

A

P

G

G

Y

T

P

P

E

V

R

R

M

K

R

R

-

G

-

N

V

S

E

R

F

Y

A

S

T

P

A

L

L

G

D

D

V

M

M

L

V

C

E

E

S

A

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

K

G

G

F

W

Y

Y

R

Q

Y

Y

E

D

A

K

D

P

A

L

A

G

G

R

H

I

Q

H

R

K

K

P

V

-

V

-

D

D

P

P

D

W

V

L

G

S

A

T

L

F

L

L

A

S

T

Q

-

Y

-

R

-

E

V

V

Q

H

P

H

G

I

G

E

S

Q

R

R

I

T

I

I

S

S

D

K

E

E

E

E

I

I

V

L

Q

E

R

R

T

C

M

L

L

Y

P

S

M

L

I

I

L

N

E

E

T

A

A

F

R

R

C

I

L

L

E

E

D

E

G

G

V

M

V

A

G

A

S

R

A

P

A

E

E

H

A

I

D

D

M

V

A

I

L

Y

I

L

L

H

G

G

I

Y

G

G

F

W

P

P

R

R

H

H

L

K

G

G

G

G

A

P

L

M

H

F

Y

Y

A

A

D

A

E

S

L

V

G

G

A

L

K

P

T

T

I

V

M

L

E

E

W

K

C

L

D

Q

R

K

Y

Y

A

Y

N

R

L

Q

G

N

-

P

-

D

-

I

P

P

L

Q

Y

L

H

E

P

P

T

S

P

D

R

Y

M

L

R

R

R

R

L

L

A

V

V

A

D

Q

G

G

A

S

active site: A67 (= A68), F72 (= F73), G82 (= G78), G106 (= G116), E109 (= E119), P128 (= P138), E129 (= E139), G137 (= G147), K255 (≠ A269), S409 (= S427), H430 (= H448), E442 (= E460), N480 (= N498)
binding coenzyme a: V27 (= V28), A65 (≠ V66), D68 (= D69), I69 (= I70), P128 (= P138), Y162 (≠ P172), F277 (= F291), K281 (≠ Q295)
binding nicotinamide-adenine-dinucleotide: G309 (= G318), G311 (= G320), T312 (≠ I321), M313 (= M322), E332 (≠ D341), S333 (≠ I342), Q337 (≠ A346), A379 (= A397), V380 (= V398), F381 (≠ V399), E382 (= E400), K387 (= K405), N407 (= N425), S409 (= S427), H430 (= H448)
binding nicotinamide: A67 (= A68), E109 (= E119), E129 (= E139), P136 (= P146), F261 (= F275)

Sites not aligning to the query:

binding coenzyme a: 26

5omoA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with with 3s-hydroxy-decanoyl-coa and 3-keto- decanoyl-coa
33% identity, 95% coverage: 28:709/721 of query aligns to 26:710/725 of 5omoA

query
sites
5omoA

V
|
V

N

N

K
x
A

L

V

D

S

R
x
P

L

T

A

V

L

I

D

R

E

E

L

V

N

R

R

N

A

G

L

L

C

Q

L

K

V

A

R

G

E

S

D

D

R

H

T

T

V

V

T

K

G

A

L

I

L

V

I

I

T

C

S

G

G

A

K

N

D

G

S

N

F

F

I

C

V
x
A

G

G

A
|
A

D
|
D

I
|
I

F

H

E

G

F
|
F

T

S

D

A

L

F

-

T

-

P

-

G

-

L

-

A

F

L

G
|
G

M

S

A

L

E

V

D

D

E

E

I

I

A

Q

I

R

H

Y

N

Q

A

K

G

-

Q

-

S

-

A

-

V

-

I

-

T

-

A

-

L

-

A

-

D

-

L

-

P

-

V

-

P

P

T

V

V

L

V

A

A

A

V

I

N

Q

G

G

L

V

A

A

L
|
L

G

G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

G

A

C

D

H

V

Y

R

R

M

I

L

A

S

N

D

A

S

K

A

A

R

R

I

V

G

G

L

L

P
|
P

E
|
E

V

V

G

T

L

L

G

G

I

I

F

L

P
|
P

G
|
G

F

A

G

R

G

G

T

T

V

Q

R

L

L

L

P

P

R

R

L

V

I

V

G

G

V

V

E

P

A

V

A

A

L

L

Q

D

W

L

I

I

M

T

S

S

G

G

T

K

P
x
Y

R

L

K

S

A

A

D

D

D

E

A

A

L

L

R

R

A

L

G

G

A

I

V

L

D

D

A

A

V

V

V

V

P

K

P

S

P

D

E

P

L

V

R

E

E

E

A

A

A

-

L

I

E

K

R

F

L

A

H

Q

G

K

L

I

V

I

A

D

S

K

P

P

D

-

W

-

S

I

E

E

R

P

R

R

K

R

E

I

A

F

L

N

S

K

P

P

V

V

V

P

R

S

A

L

G

-

E

P

D

N

L

M

A

D

P

S

I

V

F

F

A

A

A

E

A

A

K

I

A

A

K

K

A

V

R

R

K

K

T

Q

G

Y

P

P

H

G

L

V

P

L

A

A

S

P

L

E

A

T

V

C

V

V

E

R

L

S

I

I

E

Q

S

A

A

S

A

V

G

K

V

H

D

P

R

Y

D

E

A

V

A

G

L

I

A
x
K

L

E

E

E

A

E

R

K

A

L

F
|
F

A

M

H

Y

I

L

A

R

R

A

T

S

P

G

A

Q

A

A

R

K

A

A

L

L

V

Q

A

Y

V

A

F

F

I

F

N

A

D

E

Q
x
K

A

S

V

A

K

N

K

K

T

W

T

S

-

T

-

P

-

S

-

G

-

A

K

S

Q

W

Q

K

T

T

R

A

A

S

A

A

R

Q

P

P

V

V

S

S

R

S

A

V

A

G

V

V

L

L

G

G

A

L

G

G

I

T

M

M

G

G

G

R

G

G

I

I

A

A

Y

I

Q

S

S

F

A

A

V

R

C

V

G

G

I

I

P

S

V

V

I

V

M

A

K

V

D

E

I

S

A

D

Q

P

S

K

A

Q

L

L

D

D

L

A

G

-

M

-

T

-

Q

-

A

-

G

-

A

-

L

-

L

-

A

A

K

K

R

K

V

I

E

I

T

T

G

F

A

T

L

L

-

E

-

K

-

E

-

A

S

S

S

R

A

A

K

H

A

Q

D

N

G

G

I

-

L

Q

A

A

S

S

I

A

R

K

P

P

G

K

L

L

T

R

Y

F

D

S

G

S

-

S

-

T

-

K

-

E

I

L

A

S

E

T

A

V

D

D

V

L

I

V

V

V

E

E

A

A

V

V

V

F

E

E

N

D

I

M

A

N

I

L

K

K

R

K

A

K

V

V

L

F

A

A

E

E

L

L

E

S

E

A

H

L

I

C

R

K

P

P

D

G

A

A

V

F

V

L

A

C

T

T

N

N

T

T

S
|
S

S

A

L

L

L

N

V

V

A

D

D

D

L

I

A

A

R

S

D

S

L

T

E

D

W

R

P

P

E

Q

N

L

F

V

V

I

G

G

M

T

H
|
H

F

F

F

F

N

S

P

P

V

A

H

H

R

V

M

M

P

R

L

L

V

L

E
|
E

V

V

I

I

Q

P

G

S

P

R

H

Y

T

S

S

S

P

P

E

T

A

T

V

I

A

A

T

T

I

V

V

M

G

S

Y

L

A

S

S

K

A

K

M

I

G

G

K

K

T

I

P

G

V

V

V

V

V

V

R

G

D

N

C

C

P

Y

G

G

F

F

L

V

V

G

N
|
N

R

R

I

M

L

L

A

A

A

P

N

Y

V

Y

L

N

G

Q

F

G

L

F

R

F

L

L

I

L

H

E

D

E

G

G

A

S

D

K

F

P

L

E

R

D

I

V

D

D

A

G

A

V

M

L

E

E

G

E

F

F

G

G

W

F

P
x
K

M
|
M

G

G

P

P

A

F

W

R

L
x
V

Q

S

D

D

V

L

I

A

G

G

M

L

D

D

T

V

S

G

R

W

H

K

V

I

I

R

E

K

-

G

-

Q

-

G

-

L

-

T

-

G

-

P

V

S

I

L

A

P

A

P

G

G

Y

T

P

P

E

V

R

R

M

K

R

R

-

G

-

N

V

S

E

R

F

Y

A

S

T

P

A

L

L

G

D

D

V

M

M

L

V

C

E

E

S

A

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

K

G

G

F

W

Y

Y

R

Q

Y

Y

E

D

A

K

D

P

A

L

A

G

G

R

H

I

Q

H

R

K

K

P

V

-

V

-

D

D

P

P

D

W

V

L

G

S

A

T

L

F

L

L

A

S

T

Q

-

Y

-

R

-

E

V

V

Q

H

P

H

G

I

G

E

S

Q

R

R

I

T

I

I

S

S

D

K

E

E

E

E

I

I

V

L

Q

E

R

R

T

C

M

L

L

Y

P

S

M

L

I

I

L

N

E

E

T

A

A

F

R

R

C

I

L

L

E

E

D

E

G

G

V

M

V

A

G

A

S

R

A

P

A

E

E

H

A

I

D

D

M

V

A

I

L

Y

I

L

L

H

G

G

I
x
Y

G

G

F

W

P

P

R

R

H

H

L

K

G

G

G

G

A

P

L

M

H

F

Y

Y

A

A

D

A

E

S

L

V

G

G

A

L

K

P

T

T

I

V

M

L

E

E

W

K

C

L

D

Q

R

K

Y

Y

A

Y

N

R

L

Q

G

N

-

P

-

D

-

I

P

P

L

Q

Y

L

H

E

P

P

T

S

P

D

R

Y

M

L

R

R

R

R

L

L

A

V

V

A

D

Q

G

G

A

S

active site: A66 (= A68), F71 (= F73), G81 (= G78), G105 (= G116), E108 (= E119), P127 (= P138), E128 (= E139), P135 (= P146), G136 (= G147), K254 (≠ A269), S415 (= S427), H436 (= H448), E448 (= E460), N486 (= N498)
binding (s)-3-hydroxydecanoyl-coa: V26 (= V28), A28 (≠ K30), P31 (≠ R33), A64 (≠ V66), A66 (= A68), D67 (= D69), I68 (= I70), L103 (= L114), G105 (= G116), E108 (= E119), P127 (= P138), E128 (= E139), Y161 (≠ P172), F260 (= F275), K280 (≠ Q295)
binding 3-keto-decanoyl-coa: S415 (= S427), N486 (= N498), K519 (≠ P531), M520 (= M532), V525 (≠ L537), Y658 (≠ I660)

Sites not aligning to the query:

binding (s)-3-hydroxydecanoyl-coa: 25

5mgbA Crystal structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and NAD (see paper)
33% identity, 95% coverage: 28:709/721 of query aligns to 26:710/725 of 5mgbA

query
sites
5mgbA

V
|
V

N

N

K

A

L

V

D

S

R

P

L

T

A

V

L

I

D

R

E

E

L

V

N

R

R

N

A

G

L

L

C

Q

L

K

V

A

R

G

E

S

D

D

R

H

T

T

V

V

T

K

G

A

L

I

L

V

I

I

T

C

S

G

G

A

K

N

D

G

S

N

F

F

I

C

V
x
A

G
|
G

A
|
A

D
|
D

I
|
I

F

H

E

G

F
|
F

T

S

D

A

L

F

-

T

-

P

-

G

-

L

-

A

F

L

G
|
G

M

S

A

L

E

V

D

D

E

E

I

I

A

Q

I

R

H

Y

N

Q

A

K

G

-

Q

-

S

-

A

-

V

-

I

-

T

-

A

-

L

-

A

-

D

-

L

-

P

-

V

-

P

P

T

V

V

L

V

A

A

A

V

I

N

Q

G

G

L

V

A

A

L

L

G

G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

G

A

C

D

H

V

Y

R

R

M

I

L

A

S

N

D

A

S

K

A

A

R

R

I

V

G

G

L

L

P
|
P

E
|
E

V

V

G

T

L

L

G

G

I

I

F

L

P
|
P

G
|
G

F

A

G

R

G

G

T

T

V

Q

R

L

L

L

P

P

R

R

L

V

I

V

G

G

V

V

E

P

A

V

A

A

L

L

Q

D

W

L

I

I

M

T

S

S

G

G

T

K

P
x
Y

R

L

K

S

A

A

D

D

D

E

A

A

L

L

R

R

A

L

G

G

A

I

V

L

D

D

A

A

V

V

V

V

P

K

P

S

P

D

E

P

L

V

R

E

E

E

A

A

A

-

L

I

E

K

R

F

L

A

H

Q

G

K

L

I

V

I

A

D

S

K

P

P

D

-

W

-

S

I

E

E

R

P

R

R

K

R

E

I

A

F

L

N

S

K

P

P

V

V

V

P

R

S

A

L

G

-

E

P

D

N

L

M

A

D

P

S

I

V

F

F

A

A

A

E

A

A

K

I

A

A

K

K

A

V

R

R

K

K

T

Q

G

Y

P

P

H

G

L

V

P

L

A

A

S

P

L

E

A

T

V

C

V

V

E

R

L

S

I

I

E

Q

S

A

A

S

A

V

G

K

V

H

D

P

R

Y

D

E

A

V

A

G

L

I

A
x
K

L

E

E

E

A

E

R

K

A

L

F

F

A

M

H

Y

I

L

A

R

R

A

T

S

P

G

A

Q

A

A

R

K

A

A

L

L

V

Q

A

Y

V

A

F

F

I

F

N

A

D

E

Q

K

A

S

V

A

K

N

K

K

T

W

T

S

-

T

-

P

-

S

-

G

-

A

K

S

Q

W

Q

K

T

T

R

A

A

S

A

A

R

Q

P

P

V

V

S

S

R

S

A

V

A

G

V

V

L
|
L

G
|
G

A

L

G
|
G

I
x
T

M
|
M

G

G

G

R

G

G

I

I

A

A

Y

I

Q

S

S

F

A

A

V

R

C

V

G

G

I

I

P

S

V

V

I

V

M

A

K

V

D
x
E

I
x
S

A

D

Q

P

S

K

A
x
Q

L

L

D

D

L

A

G

-

M

-

T

-

Q

-

A

-

G

-

A

-

L

-

L

-

A

A

K

K

R

K

V

I

E

I

T

T

G

F

A

T

L

L

-

E

-

K

-

E

-

A

S

S

S

R

A

A

K

H

A

Q

D

N

G

G

I

-

L

Q

A

A

S

S

I

A

R

K

P

P

G

K

L

L

T

R

Y

F

D

S

G

S

-

S

-

T

-

K

-

E

I

L

A

S

E

T

A

V

D

D

V

L

I

V

V

V

E

E

A

A

V
|
V

V
x
F

E
|
E

N

D

I

M

A

N

I

L

K

K

R

K

A

K

V

V

L

F

A

A

E

E

L

L

E

S

E

A

H

L

I

C

R

K

P

P

D

G

A

A

V

F

V

L

A

C

T

T

N
|
N

T

T

S
|
S

S

A

L

L

L

N

V

V

A

D

D

D

L

I

A

A

R

S

D

S

L

T

E

D

W

R

P

P

E

Q

N

L

F

V

V

I

G

G

M

T

H
|
H

F

F

F

F

N

S

P

P

V

A

H

H

R

V

M

M

P

R

L

L

V

L

E
|
E

V

V

I

I

Q

P

G

S

P

R

H

Y

T

S

S

S

P

P

E

T

A

T

V

I

A

A

T

T

I

V

V

M

G

S

Y

L

A

S

S

K

A

K

M

I

G

G

K

K

T

I

P

G

V

V

V

V

V

V

R

G

D

N

C

C

P

Y

G

G

F

F

L

V

V

G

N
|
N

R

R

I

M

L

L

A

A

A

P

N

Y

V

Y

L

N

G

Q

F

G

L

F

R

F

L

L

I

L

H

E

D

E

G

G

A

S

D

K

F

P

L

E

R

D

I

V

D

D

A

G

A

V

M

L

E

E

G

E

F

F

G

G

W

F

P

K

M

M

G

G

P

P

A

F

W

R

L

V

Q

S

D

D

V

L

I

A

G

G

M

L

D

D

T

V

S

G

R

W

H

K

V

I

I

R

E

K

-

G

-

Q

-

G

-

L

-

T

-

G

-

P

V

S

I

L

A

P

A

P

G

G

Y

T

P

P

E

V

R

R

M

K

R

R

-

G

-

N

V

S

E

R

F

Y

A

S

T

P

A

L

L

G

D

D

V

M

M

L

V

C

E

E

S

A

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

K

G

G

F

W

Y

Y

R

Q

Y

Y

E

D

A

K

D

P

A

L

A

G

G

R

H

I

Q

H

R

K

K

P

V

-

V

-

D

D

P

P

D

W

V

L

G

S

A

T

L

F

L

L

A

S

T

Q

-

Y

-

R

-

E

V

V

Q

H

P

H

G

I

G

E

S

Q

R

R

I

T

I

I

S

S

D

K

E

E

E

E

I

I

V

L

Q

E

R

R

T

C

M

L

L

Y

P

S

M

L

I

I

L

N

E

E

T

A

A

F

R

R

C

I

L

L

E

E

D

E

G

G

V

M

V

A

G

A

S

R

A

P

A

E

E

H

A

I

D

D

M

V

A

I

L

Y

I

L

L

H

G

G

I

Y

G

G

F

W

P

P

R

R

H

H

L

K

G

G

G

G

A

P

L

M

H

F

Y

Y

A

A

D

A

E

S

L

V

G

G

A

L

K

P

T

T

I

V

M

L

E

E

W

K

C

L

D

Q

R

K

Y

Y

A

Y

N

R

L

Q

G

N

-

P

-

D

-

I

P

P

L

Q

Y

L

H

E

P

P

T

S

P

D

R

Y

M

L

R

R

R

R

L

L

A

V

V

A

D

Q

G

G

A

S

active site: A66 (= A68), F71 (= F73), G81 (= G78), G105 (= G116), E108 (= E119), P127 (= P138), E128 (= E139), P135 (= P146), G136 (= G147), K254 (≠ A269), S415 (= S427), H436 (= H448), E448 (= E460), N486 (= N498)
binding acetoacetyl-coenzyme a: V26 (= V28), A64 (≠ V66), G65 (= G67), A66 (= A68), D67 (= D69), I68 (= I70), G105 (= G116), E128 (= E139), Y161 (≠ P172)
binding nicotinamide-adenine-dinucleotide: L307 (= L317), G308 (= G318), G310 (= G320), T311 (≠ I321), M312 (= M322), E331 (≠ D341), S332 (≠ I342), Q336 (≠ A346), V386 (= V398), F387 (≠ V399), E388 (= E400), N413 (= N425), S415 (= S427), H436 (= H448)

Sites not aligning to the query:

binding acetoacetyl-coenzyme a: 25

3zwcA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 3s-hydroxy-decanoyl-coa (see paper)
33% identity, 95% coverage: 28:709/721 of query aligns to 26:710/725 of 3zwcA

query
sites
3zwcA

V
|
V

N

N

K

A

L

V

D

S

R

P

L

T

A

V

L

I

D

R

E

E

L

V

N

R

R

N

A

G

L

L

C

Q

L

K

V

A

R

G

E

S

D

D

R

H

T

T

V

V

T

K

G

A

L

I

L

V

I

I

T

C

S

G

G

A

K

N

D

G

S

N

F

F

I

C

V
x
A

G
|
G

A
|
A

D
|
D

I
|
I

F

H

E

G

F
|
F

T

S

D

A

L

F

-

T

-

P

-
x
G

-
x
L

-

A

F
x
L

G
|
G

M

S

A

L

E

V

D

D

E

E

I

I

A

Q

I

R

H

Y

N

Q

A

K

G

-

Q

-

S

-

A

-

V

-

I

-

T

-

A

-

L

-

A

-

D

-

L

-

P

-

V

-

P

P

T

V

V

L

V

A

A

A

V

I

N

Q

G

G

L
x
V

A

A

L

L

G
|
G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

G

A

C

D

H

V

Y

R

R

M

I

L

A

S

N

D

A

S

K

A

A

R

R

I

V

G

G

L

L

P
|
P

E
|
E

V

V

G

T

L

L

G

G

I

I

F

L

P
|
P

G
|
G

F

A

G

R

G

G

T

T

V

Q

R

L

L

L

P

P

R

R

L

V

I

V

G

G

V

V

E

P

A

V

A

A

L

L

Q

D

W

L

I

I

M

T

S

S

G

G

T

K

P

Y

R

L

K

S

A

A

D

D

D

E

A

A

L

L

R

R

A

L

G

G

A

I

V

L

D

D

A

A

V

V

V

V

P

K

P

S

P

D

E

P

L

V

R

E

E

E

A

A

A

-

L

I

E

K

R

F

L

A

H

Q

G

K

L

I

V

I

A

D

S

K

P

P

D

-

W

-

S

I

E

E

R

P

R

R

K

R

E

I

A

F

L

N

S

K

P

P

V

V

V

P

R

S

A

L

G

-

E

P

D

N

L

M

A

D

P

S

I

V

F

F

A

A

A

E

A

A

K

I

A

A

K

K

A

V

R

R

K

K

T

Q

G

Y

P

P

H

G

L

V

P

L

A

A

S

P

L

E

A

T

V

C

V

V

E

R

L

S

I

I

E

Q

S

A

A

S

A

V

G

K

V

H

D

P

R

Y

D

E

A

V

A

G

L

I

A
x
K

L

E

E

E

A

E

R

K

A

L

F
|
F

A

M

H

Y

I

L

A

R

R

A

T

S

P

G

A

Q

A

A

R

K

A

A

L

L

V

Q

A

Y

V

A

F

F

I

F

N

A

D

E

Q

K

A

S

V

A

K

N

K

K

T

W

T

S

-

T

-

P

-

S

-

G

-

A

K

S

Q

W

Q

K

T

T

R

A

A

S

A

A

R

Q

P

P

V

V

S

S

R

S

A

V

A

G

V

V

L

L

G
|
G

A

L

G
|
G

I
x
T

M
|
M

G

G

G

R

G

G

I

I

A

A

Y

I

Q

S

S

F

A

A

V

R

C

V

G

G

I

I

P

S

V

V

I

V

M

A

K

V

D
x
E

I

S

A

D

Q

P

S

K

A
x
Q

L

L

D

D

L

A

G

-

M

-

T

-

Q

-

A

-

G

-

A

-

L

-

L

-

A

A

K

K

R

K

V

I

E

I

T

T

G

F

A

T

L

L

-

E

-

K

-

E

-

A

S

S

S

R

A

A

K

H

A

Q

D

N

G

G

I

-

L

Q

A

A

S

S

I

A

R

K

P

P

G

K

L

L

T

R

Y

F

D

S

G

S

-

S

-

T

-

K

-

E

I

L

A

S

E

T

A

V

D

D

V

L

I

V

V

V

E

E

A
|
A

V
|
V

V
x
F

E
|
E

N

D

I

M

A

N

I

L

K
|
K

R

K

A

K

V

V

L

F

A

A

E

E

L

L

E

S

E

A

H

L

I

C

R

K

P

P

D

G

A

A

V

F

V

L

A

C

T

T

N
|
N

T

T

S
|
S

S

A

L

L

L

N

V

V

A

D

D

D

L

I

A

A

R

S

D

S

L

T

E

D

W

R

P

P

E

Q

N

L

F

V

V

I

G

G

M

T

H
|
H

F

F

F

F

N

S

P

P

V

A

H

H

R

V

M

M

P

R

L

L

V

L

E
|
E

V

V

I

I

Q

P

G

S

P

R

H

Y

T

S

S

S

P

P

E

T

A

T

V

I

A

A

T

T

I

V

V

M

G

S

Y

L

A

S

S

K

A

K

M

I

G

G

K

K

T

I

P

G

V

V

V

V

V

V

R

G

D

N

C

C

P

Y

G

G

F

F

L

V

V

G

N
|
N

R

R

I

M

L

L

A

A

A

P

N

Y

V

Y

L

N

G

Q

F

G

L

F

R

F

L

L

I

L

H

E

D

E

G

G

A

S

D

K

F

P

L

E

R

D

I

V

D

D

A

G

A

V

M

L

E

E

G

E

F

F

G

G

W

F

P

K

M

M

G

G

P

P

A

F

W

R

L

V

Q

S

D

D

V

L

I

A

G

G

M

L

D

D

T

V

S

G

R

W

H

K

V

I

I

R

E

K

-

G

-

Q

-

G

-

L

-

T

-

G

-

P

V

S

I

L

A

P

A

P

G

G

Y

T

P

P

E

V

R

R

M

K

R

R

-

G

-

N

V

S

E

R

F

Y

A

S

T

P

A

L

L

G

D

D

V

M

M

L

V

C

E

E

S

A

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

K

G

G

F

W

Y

Y

R

Q

Y

Y

E

D

A

K

D

P

A

L

A

G

G

R

H

I

Q

H

R

K

K

P

V

-

V

-

D

D

P

P

D

W

V

L

G

S

A

T

L

F

L

L

A

S

T

Q

-

Y

-

R

-

E

V

V

Q

H

P

H

G

I

G

E

S

Q

R

R

I

T

I

I

S

S

D

K

E

E

E

E

I

I

V

L

Q

E

R

R

T

C

M

L

L

Y

P

S

M

L

I

I

L

N

E

E

T

A

A

F

R

R

C

I

L

L

E

E

D

E

G

G

V

M

V

A

G

A

S

R

A

P

A

E

E

H

A

I

D

D

M

V

A

I

L

Y

I

L

L

H

G

G

I

Y

G

G

F

W

P

P

R

R

H

H

L

K

G

G

G

G

A

P

L

M

H

F

Y

Y

A

A

D

A

E

S

L

V

G

G

A

L

K

P

T

T

I

V

M

L

E

E

W

K

C

L

D

Q

R

K

Y

Y

A

Y

N

R

L

Q

G

N

-

P

-

D

-

I

P

P

L

Q

Y

L

H

E

P

P

T

S

P

D

R

Y

M

L

R

R

R

R

L

L

A

V

V

A

D

Q

G

G

A

S

active site: A66 (= A68), F71 (= F73), G81 (= G78), G105 (= G116), E108 (= E119), P127 (= P138), E128 (= E139), P135 (= P146), G136 (= G147), K254 (≠ A269), S415 (= S427), H436 (= H448), E448 (= E460), N486 (= N498)
binding (s)-3-hydroxydecanoyl-coa: V26 (= V28), A64 (≠ V66), G65 (= G67), A66 (= A68), D67 (= D69), I68 (= I70), G77 (vs. gap), L78 (vs. gap), L80 (≠ F77), V101 (≠ L112), G104 (= G115), G105 (= G116), E108 (= E119), E128 (= E139), F260 (= F275)
binding nicotinamide-adenine-dinucleotide: G308 (= G318), G310 (= G320), T311 (≠ I321), M312 (= M322), E331 (≠ D341), Q336 (≠ A346), A385 (= A397), V386 (= V398), F387 (≠ V399), E388 (= E400), K393 (= K405), N413 (= N425), S415 (= S427), H436 (= H448)

2x58A The crystal structure of mfe1 liganded with coa (see paper)
33% identity, 95% coverage: 28:709/721 of query aligns to 26:710/725 of 2x58A

query
sites
2x58A

V
|
V

N

N

K
x
A

L

V

D

S

R

P

L

T

A

V

L

I

D

R

E

E

L

V

N

R

R

N

A

G

L

L

C

Q

L

K

V

A

R

G

E

S

D

D

R

H

T

T

V

V

T

K

G

A

L

I

L

V

I

I

T

C

S

G

G

A

K

N

D

G

S

N

F

F

I

C

V
x
A

G

G

A
|
A

D
|
D

I
|
I

F

H

E

G

F
|
F

T

S

D

A

L

F

-

T

-

P

-

G

-

L

-

A

F

L

G
|
G

M

S

A

L

E

V

D

D

E

E

I

I

A

Q

I

R

H

Y

N

Q

A

K

G

-

Q

-

S

-

A

-

V

-

I

-

T

-

A

-

L

-

A

-

D

-

L

-

P

-

V

-

P

P

T

V

V

L

V

A

A

A

V

I

N

Q

G

G

L

V

A

A

L

L

G

G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

G

A

C

D

H

V

Y

R

R

M

I

L

A

S

N

D

A

S

K

A

A

R

R

I

V

G

G

L

L

P
|
P

E
|
E

V

V

G

T

L

L

G

G

I

I

F

L

P
|
P

G
|
G

F

A

G

R

G

G

T

T

V

Q

R

L

L

L

P

P

R

R

L

V

I

V

G

G

V

V

E

P

A

V

A

A

L

L

Q

D

W

L

I

I

M

T

S

S

G

G

T

K

P

Y

R

L

K

S

A

A

D

D

D

E

A

A

L

L

R

R

A

L

G

G

A

I

V

L

D

D

A

A

V

V

V

V

P

K

P

S

P

D

E

P

L

V

R

E

E

E

A

A

A

-

L

I

E

K

R

F

L

A

H

Q

G

K

L

I

V

I

A

D

S

K

P

P

D

-

W

-

S

I

E

E

R

P

R

R

K

R

E

I

A

F

L

N

S

K

P

P

V

V

V

P

R

S

A

L

G

-

E

P

D

N

L

M

A

D

P

S

I

V

F

F

A

A

A

E

A

A

K

I

A

A

K

K

A

V

R

R

K

K

T

Q

G

Y

P

P

H

G

L

V

P

L

A

A

S

P

L

E

A

T

V

C

V

V

E

R

L

S

I

I

E

Q

S

A

A

S

A

V

G

K

V

H

D

P

R

Y

D

E

A

V

A

G

L

I

A
x
K

L

E

E

E

A

E

R

K

A

L

F

F

A

M

H

Y

I

L

A

R

R

A

T

S

P

G

A

Q

A

A

R

K

A

A

L

L

V

Q

A

Y

V

A

F

F

I

F

N

A

D

E

Q

K

A

S

V

A

K

N

K

K

T

W

T

S

-

T

-

P

-

S

-

G

-

A

K

S

Q

W

Q

K

T

T

R

A

A

S

A

A

R

Q

P

P

V

V

S

S

R

S

A

V

A

G

V

V

L

L

G

G

A

L

G
|
G

I
x
T

M
|
M

G

G

G

R

G

G

I

I

A

A

Y

I

Q

S

S

F

A

A

V

R

C

V

G

G

I

I

P

S

V

V

I

V

M

A

K

V

D
x
E

I
x
S

A

D

Q

P

S

K

A
x
Q

L

L

D

D

L

A

G

-

M

-

T

-

Q

-

A

-

G

-

A

-

L

-

L

-

A

A

K

K

R

K

V

I

E

I

T

T

G

F

A

T

L

L

-

E

-

K

-

E

-

A

S

S

S

R

A

A

K

H

A

Q

D

N

G

G

I

-

L

Q

A

A

S

S

I

A

R

K

P

P

G

K

L

L

T

R

Y

F

D

S

G

S

-

S

-

T

-

K

-

E

I

L

A

S

E

T

A

V

D

D

V

L

I

V

V

V

E

E

A

A

V
|
V

V

F

E

E

N

D

I

M

A

N

I
x
L

K

K

R

K

A

K

V

V

L

F

A

A

E

E

L

L

E

S

E

A

H

L

I

C

R

K

P

P

D

G

A

A

V

F

V

L

A

C

T

T

N

N

T

T

S
|
S

S

A

L

L

L

N

V

V

A

D

D

D

L

I

A

A

R

S

D

S

L

T

E

D

W

R

P

P

E

Q

N

L

F

V

V

I

G

G

M

T

H
|
H

F

F

F

F

N

S

P

P

V

A

H

H

R

V

M

M

P

R

L

L

V

L

E
|
E

V

V

I

I

Q

P

G

S

P

R

H

Y

T

S

S

S

P

P

E

T

A

T

V

I

A

A

T

T

I

V

V

M

G

S

Y

L

A

S

S

K

A

K

M

I

G

G

K

K

T

I

P

G

V

V

V

V

V

V

R

G

D

N

C

C

P

Y

G

G

F

F

L

V

V

G

N
|
N

R

R

I

M

L

L

A

A

A

P

N

Y

V

Y

L

N

G

Q

F

G

L

F

R

F

L

L

I

L

H

E

D

E

G

G

A

S

D

K

F

P

L

E

R

D

I

V

D

D

A

G

A

V

M

L

E

E

G

E

F

F

G

G

W

F

P

K

M

M

G

G

P

P

A

F

W

R

L

V

Q

S

D

D

V

L

I

A

G

G

M

L

D

D

T

V

S

G

R

W

H

K

V

I

I

R

E

K

-

G

-

Q

-

G

-

L

-

T

-

G

-

P

V

S

I

L

A

P

A

P

G

G

Y

T

P

P

E

V

R

R

M

K

R

R

-

G

-

N

V

S

E

R

F

Y

A

S

T

P

A

L

L

G

D

D

V

M

M

L

V

C

E

E

S

A

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

K

G

G

F

W

Y

Y

R

Q

Y

Y

E

D

A

K

D

P

A

L

A

G

G

R

H

I

Q

H

R

K

K

P

V

-

V

-

D

D

P

P

D

W

V

L

G

S

A

T

L

F

L

L

A

S

T

Q

-

Y

-

R

-

E

V

V

Q

H

P

H

G

I

G

E

S

Q

R

R

I

T

I

I

S

S

D

K

E

E

E

E

I

I

V

L

Q

E

R

R

T

C

M

L

L

Y

P

S

M

L

I

I

L

N

E

E

T

A

A

F

R

R

C

I

L

L

E

E

D

E

G

G

V

M

V

A

G

A

S

R

A

P

A

E

E

H

A

I

D

D

M

V

A

I

L

Y

I

L

L

H

G

G

I

Y

G

G

F

W

P

P

R

R

H

H

L

K

G

G

G

G

A

P

L

M

H

F

Y

Y

A

A

D

A

E

S

L

V

G

G

A

L

K

P

T

T

I

V

M

L

E

E

W

K

C

L

D

Q

R

K

Y

Y

A

Y

N

R

L

Q

G

N

-

P

-

D

-

I

P

P

L

Q

Y

L

H

E

P

P

T

S

P

D

R

Y

M

L

R

R

R

R

L

L

A

V

V

A

D

Q

G

G

A

S

active site: A66 (= A68), F71 (= F73), G81 (= G78), G105 (= G116), E108 (= E119), P127 (= P138), E128 (= E139), P135 (= P146), G136 (= G147), K254 (≠ A269), S415 (= S427), H436 (= H448), E448 (= E460), N486 (= N498)
binding adenosine-5'-diphosphate: G310 (= G320), T311 (≠ I321), M312 (= M322), E331 (≠ D341), S332 (≠ I342), Q336 (≠ A346), V386 (= V398), L392 (≠ I404)
binding coenzyme a: V26 (= V28), A28 (≠ K30), A64 (≠ V66), A66 (= A68), D67 (= D69), I68 (= I70), E128 (= E139)

6zicAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with 3s-hydroxybutanoyl-coa and nadh' (see paper)
33% identity, 95% coverage: 28:709/721 of query aligns to 26:708/723 of 6zicAAA

query
sites
6zicAAA

V
|
V

N

N

K
x
A

L

V

D

S

R

P

L

T

A

V

L

I

D

R

E

E

L

V

N

R

R

N

A

G

L

L

C

Q

L

K

V

A

R

G

E

S

D

D

R

H

T

T

V

V

T

K

G

A

L

I

L

V

I

I

T

C

S

G

G

A

K

N

D

G

S

N

F

F

I

C

V

A

G

G

A
|
A

D
|
D

I
|
I

F

H

E

G

F
|
F

T

S

D

A

L

F

-

T

-

P

-

G

-

L

-

A

F

L

G
|
G

M

S

A

L

E

V

D

D

E

E

I

I

A

Q

I

R

H

Y

N

Q

A

K

G

-

Q

-

S

-

A

-

V

-

I

-

T

-

A

-

L

-

A

-

D

-

L

-

P

-

V

-

P

P

T

V

V

L

V

A

A

A

V

I

N

Q

G

G

L

V

A

A

L

L

G
|
G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

G

A

C

D

H

V

Y

R

R

M

I

L

A

S

N

D

A

S

K

A

A

R

R

I

V

G

G

L

L

P
|
P

E
|
E

V

V

G

T

L

L

G

G

I

I

F

L

P

P

G
|
G

F

A

G

R

G

G

T

T

V

Q

R

L

L

L

P

P

R

R

L

V

I

V

G

G

V

V

E

P

A

V

A

A

L

L

Q

D

W

L

I

I

M

T

S

S

G

G

T

K

P
x
Y

R

L

K

S

A

A

D

D

D

E

A

A

L

L

R

R

A

L

G

G

A

I

V

L

D

D

A

A

V

V

V

V

P

K

P

S

P

D

E

P

L

V

R

E

E

E

A

A

A

-

L

I

E

K

R

F

L

A

H

Q

G

K

L

I

V

I

A

D

S

K

P

P

D

-

W

-

S

I

E

E

R

P

R

R

K

R

E

I

A

F

L

N

S

K

P

P

V

V

V

P

R

S

A

L

G

-

E

P

D

N

L

M

A

D

P

S

I

V

F

F

A

A

A

E

A

A

K

I

A

A

K

K

A

V

R

R

K

K

T

Q

G

Y

P

P

H

G

L

V

P

L

A

A

S

P

L

E

A

T

V

C

V

V

E

R

L

S

I

I

E

Q

S

A

A

S

A

V

G

K

V

H

D

P

R

Y

D

E

A

V

A

G

L

I

A
x
K

L

E

E

E

A

E

R

K

A

L

F

F

A

M

H

Y

I

L

A

R

R

A

T

S

P

G

A

Q

A

A

R

K

A

A

L

L

V

Q

A

Y

V

A

F

F

I

F

N

A

D

E

Q

K

A

S

V

A

K

N

K

K

T

W

T

S

-

T

-

P

-

S

-

G

-

A

K

S

Q

W

Q

K

T

T

R

A

A

S

A

A

R

Q

P

P

V

V

S

S

R

S

A

V

A

G

V

V

L

L

G
|
G

A

L

G
|
G

I
x
T

M
|
M

G

G

G

R

G

G

I

I

A

A

Y

I

Q

S

S

F

A

A

V

R

C

V

G

G

I

I

P

S

V

V

I

V

M

A

K

V

D
x
E

I
x
S

A

D

Q

P

S

K

A
x
Q

L

L

D

D

L

A

G

-

M

-

T

-

Q

-

A

-

G

-

A

-

L

-

L

-

A

A

K

K

R

K

V

I

E

I

T

T

G

F

A

T

L

L

-

E

-

K

-

E

-

A

S

S

S

R

A

A

K

H

A

Q

D

N

G

G

I

-

L

-

A

-

S

S

I

A

R

K

P

P

G

K

L

L

T

R

Y

F

D

S

G

S

-

S

-

T

-

K

-

E

I

L

A

S

E

T

A

V

D

D

V

L

I

V

V

V

E

E

A
|
A

V
|
V

V
x
F

E
|
E

N

D

I

M

A

N

I
x
L

K
|
K

R

K

A

K

V

V

L

F

A

A

E

E

L

L

E

S

E

A

H

L

I

C

R

K

P

P

D

G

A

A

V

F

V

L

A

C

T

T

N
|
N

T

T

S
|
S

S

A

L

L

L

N

V

V

A

D

D

D

L

I

A

A

R

S

D

S

L

T

E

D

W

R

P

P

E

Q

N

L

F

V

V

I

G

G

M

T

H
|
H

F

F

F

F

N

S

P

P

V

A

H

H

R

V

M

M

P

R

L

L

V

L

E
|
E

V

V

I

I

Q

P

G

S

P

R

H

Y

T

S

S

S

P

P

E

T

A

T

V

I

A

A

T

T

I

V

V

M

G

S

Y

L

A

S

S

K

A

K

M

I

G

G

K

K

T

I

P

G

V

V

V

V

V

V

R

G

D

N

C

C

P

Y

G

G

F

F

L

V

V

G

N
|
N

R

R

I

M

L

L

A

A

A

P

N

Y

V

Y

L

N

G

Q

F

G

L

F

R

F

L

L

I

L

H

E

D

E

G

G

A

S

D

K

F

P

L

E

R

D

I

V

D

D

A

G

A

V

M

L

E

E

G

E

F

F

G

G

W

F

P

K

M

M

G

G

P

P

A

F

W

R

L

V

Q

S

D

D

V

L

I

A

G

G

M

L

D

D

T

V

S

G

R

W

H

K

V

I

I

R

E

K

-

G

-

Q

-

G

-

L

-

T

-

G

-

P

V

S

I

L

A

P

A

P

G

G

Y

T

P

P

E

V

R

R

M

K

R

R

-

G

-

N

V

S

E

R

F

Y

A

S

T

P

A

L

L

G

D

D

V

M

M

L

V

C

E

E

S

A

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

K

G

G

F

W

Y

Y

R

Q

Y

Y

E

D

A

K

D

P

A

L

A

G

G

R

H

I

Q

H

R

K

K

P

V

-

V

-

D

D

P

P

D

W

V

L

G

S

A

T

L

F

L

L

A

S

T

Q

-

Y

-

R

-

E

V

V

Q

H

P

H

G

I

G

E

S

Q

R

R

I

T

I

I

S

S

D

K

E

E

E

E

I

I

V

L

Q

E

R

R

T

C

M

L

L

Y

P

S

M

L

I

I

L

N

E

E

T

A

A

F

R

R

C

I

L

L

E

E

D

E

G

G

V

M

V

A

G

A

S

R

A

P

A

E

E

H

A

I

D

D

M

V

A

I

L

Y

I

L

L

H

G

G

I

Y

G

G

F

W

P

P

R

R

H

H

L

K

G

G

G

G

A

P

L

M

H

F

Y

Y

A

A

D

A

E

S

L

V

G

G

A

L

K

P

T

T

I

V

M

L

E

E

W

K

C

L

D

Q

R

K

Y

Y

A

Y

N

R

L

Q

G

N

-

P

-

D

-

I

P

P

L

Q

Y

L

H

E

P

P

T

S

P

D

R

Y

M

L

R

R

R

R

L

L

A

V

V

A

D

Q

G

G

A

S

active site: A66 (= A68), F71 (= F73), G81 (= G78), G105 (= G116), E108 (= E119), P127 (= P138), E128 (= E139), G136 (= G147), K254 (≠ A269), S413 (= S427), H434 (= H448), E446 (= E460), N484 (= N498)
binding 3-hydroxybutanoyl-coenzyme a: V26 (= V28), A28 (≠ K30), A66 (= A68), D67 (= D69), I68 (= I70), G104 (= G115), G105 (= G116), E108 (= E119), E128 (= E139), Y161 (≠ P172)
binding 1,4-dihydronicotinamide adenine dinucleotide: G308 (= G318), G310 (= G320), T311 (≠ I321), M312 (= M322), E331 (≠ D341), S332 (≠ I342), Q336 (≠ A346), A383 (= A397), V384 (= V398), F385 (≠ V399), E386 (= E400), L390 (≠ I404), K391 (= K405), N411 (= N425), S413 (= S427), H434 (= H448)

Sites not aligning to the query:

binding 3-hydroxybutanoyl-coenzyme a: 25

6zibAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and nadh' (see paper)
32% identity, 95% coverage: 28:709/721 of query aligns to 26:708/723 of 6zibAAA

query
sites
6zibAAA

V
|
V

N

N

K

A

L

V

D

S

R

P

L

T

A

V

L

I

D

R

E

E

L

V

N

R

R

N

A

G

L

L

C

Q

L

K

V

A

R

G

E

S

D

D

R

H

T

T

V

V

T

K

G

A

L

I

L

V

I

I

T

C

S

G

G

A

K

N

D

G

S

N

F

F

I

C

V
x
A

G
|
G

A
|
A

D
|
D

I
|
I

F

H

E

G

F
|
F

T

S

D

A

L

F

-

T

-

P

-

G

-

L

-

A

F

L

G
|
G

M

S

A

L

E

V

D

D

E

E

I

I

A

Q

I

R

H

Y

N

Q

A

K

G

-

Q

-

S

-

A

-

V

-

I

-

T

-

A

-

L

-

A

-

D

-

L

-

P

-

V

-

P

P

T

V

V

L

V

A

A

A

V

I

N

Q

G

G

L

V

A

A

L

L

G
|
G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

G

A

C

D

H

V

Y

R

R

M

I

L

A

S

N

D

A

S

K

A

A

R

R

I

V

G

G

L

L

P
|
P

E
|
E

V

V

G

T

L

L

G

G

I

I

F

L

P

P

G
|
G

F

A

G

R

G

G

T

T

V

Q

R

L

L

L

P

P

R

R

L

V

I

V

G

G

V

V

E

P

A

V

A

A

L

L

Q

D

W

L

I

I

M

T

S

S

G

G

T

K

P
x
Y

R

L

K

S

A

A

D

D

D

E

A

A

L

L

R

R

A

L

G

G

A

I

V

L

D

D

A

A

V

V

V

V

P

K

P

S

P

D

E

P

L

V

R

E

E

E

A

A

A

-

L

I

E

K

R

F

L

A

H

Q

G

K

L

I

V

I

A

D

S

K

P

P

D

-

W

-

S

I

E

E

R

P

R

R

K

R

E

I

A

F

L

N

S

K

P

P

V

V

V

P

R

S

A

L

G

-

E

P

D

N

L

M

A

D

P

S

I

V

F

F

A

A

A

E

A

A

K

I

A

A

K

K

A

V

R

R

K

K

T

Q

G

Y

P

P

H

G

L

V

P

L

A

A

S

P

L

E

A

T

V

C

V

V

E

R

L

S

I

I

E

Q

S

A

A

S

A

V

G

K

V

H

D

P

R

Y

D

E

A

V

A

G

L

I

A
x
K

L

E

E

E

A

E

R

K

A

L

F

F

A

M

H

Y

I

L

A

R

R

A

T

S

P

G

A

Q

A

A

R

K

A

A

L

L

V

Q

A

Y

V

A

F

F

I

F

N

A

D

E

Q

K

A

S

V

A

K

N

K

K

T

W

T

S

-

T

-

P

-

S

-

G

-

A

K

S

Q

W

Q

K

T

T

R

A

A

S

A

A

R

Q

P

P

V

V

S

S

R

S

A

V

A

G

V

V

L

L

G

G

A

L

G
|
G

I
x
T

M
|
M

G

G

G

R

G

G

I

I

A

A

Y

I

Q

S

S

F

A

A

V

R

C

V

G

G

I

I

P

S

V

V

I

V

M

A

K

V

D
x
E

I
x
S

A

D

Q

P

S

K

A
x
Q

L

L

D

D

L

-

G

-

M

-

T

-

Q

A

A

A

G

K

A

K

L

I

L

I

A

T

K

F

R

T

V

L

E

E

T

K

G

E

A

A

L

S

S

R

S

A

A

H

K

Q

A

N

D

G

G

Q

I

A

L

K

A

P

S

K

I

L

R

R

P

F

G

S

L

S

T

S

Y

T

D

K

G

E

I

L

A

S

E

T

A

V

D

D

V

L

I

V

V

V

E

E

A
|
A

V
|
V

V
x
F

E
|
E

N

D

I

M

A

N

I

L

K

K

R

K

A

K

V

V

L

F

A

A

E

E

L

L

E

S

E

A

H

L

I

C

R

K

P

P

D

G

A

A

V

F

V

L

A

C

T

T

N
|
N

T

T

S
|
S

S

A

L

L

L

N

V

V

A

D

D

D

L

I

A

A

R

S

D

S

L

T

E

D

W

R

P

P

E

Q

N

L

F

V

V

I

G

G

M

T

H
|
H

F

F

F

F

N

S

P

P

V

A

H

H

R

V

M

M

P

R

L

L

V

L

E
|
E

V

V

I

I

Q

P

G

S

P

R

H

Y

T

S

S

S

P

P

E

T

A

T

V

I

A

A

T

T

I

V

V

M

G

S

Y

L

A

S

S

K

A

K

M

I

G

G

K

K

T

I

P

G

V

V

V

V

V

V

R

G

D

N

C

C

P

Y

G

G

F

F

L

V

V

G

N
|
N

R

R

I

M

L

L

A

A

A

P

N

Y

V

Y

L

N

G

Q

F

G

L

F

R

F

L

L

I

L

H

E

D

E

G

G

A

S

D

K

F

P

L

E

R

D

I

V

D

D

A

G

A

V

M

L

E

E

G

E

F

F

G

G

W

F

P

K

M

M

G

G

P

P

A

F

W

R

L

V

Q

S

D

D

V

L

I

A

G

G

M

L

D

D

T

V

S

G

R

W

H

K

V

I

I

R

E

K

-

G

-

Q

-

G

-

L

-

T

-

G

-

P

V

S

I

L

A

P

A

P

G

G

Y

T

P

P

E

V

R

R

M

K

R

R

-

G

-

N

V

S

E

R

F

Y

A

S

T

P

A

L

L

G

D

D

V

M

M

L

V

C

E

E

S

A

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

K

G

G

F

W

Y

Y

R

Q

Y

Y

E

D

A

K

D

P

A

L

A

G

G

R

H

I

Q

H

R

K

K

P

V

-

V

-

D

D

P

P

D

W

V

L

G

S

A

T

L

F

L

L

A

S

T

Q

-

Y

-

R

-

E

V

V

Q

H

P

H

G

I

G

E

S

Q

R

R

I

T

I

I

S

S

D

K

E

E

E

E

I

I

V

L

Q

E

R

R

T

C

M

L

L

Y

P

S

M

L

I

I

L

N

E

E

T

A

A

F

R

R

C

I

L

L

E

E

D

E

G

G

V

M

V

A

G

A

S

R

A

P

A

E

E

H

A

I

D

D

M

V

A

I

L

Y

I

L

L

H

G

G

I

Y

G

G

F

W

P

P

R

R

H

H

L

K

G

G

G

G

A

P

L

M

H

F

Y

Y

A

A

D

A

E

S

L

V

G

G

A

L

K

P

T

T

I

V

M

L

E

E

W

K

C

L

D

Q

R

K

Y

Y

A

Y

N

R

L

Q

G

N

-

P

-

D

-

I

P

P

L

Q

Y

L

H

E

P

P

T

S

P

D

R

Y

M

L

R

R

R

R

L

L

A

V

V

A

D

Q

G

G

A

S

active site: A66 (= A68), F71 (= F73), G81 (= G78), G105 (= G116), E108 (= E119), P127 (= P138), E128 (= E139), G136 (= G147), K254 (≠ A269), S413 (= S427), H434 (= H448), E446 (= E460), N484 (= N498)
binding acetoacetyl-coenzyme a: V26 (= V28), A64 (≠ V66), G65 (= G67), A66 (= A68), D67 (= D69), I68 (= I70), G104 (= G115), G105 (= G116), E128 (= E139), Y161 (≠ P172)
binding 1,4-dihydronicotinamide adenine dinucleotide: G310 (= G320), T311 (≠ I321), M312 (= M322), E331 (≠ D341), S332 (≠ I342), Q336 (≠ A346), A383 (= A397), V384 (= V398), F385 (≠ V399), E386 (= E400), N411 (= N425), H434 (= H448)

Sites not aligning to the query:

binding acetoacetyl-coenzyme a: 25

3zwbA Crystal structure of rat peroxisomal multifunctional enzyme type 1 (rpmfe1) complexed with 2trans-hexenoyl-coa (see paper)
33% identity, 95% coverage: 28:709/721 of query aligns to 26:710/725 of 3zwbA

query
sites
3zwbA

V
|
V

N

N

K
x
A

L

V

D

S

R

P

L

T

A

V

L

I

D

R

E

E

L

V

N

R

R

N

A

G

L

L

C

Q

L

K

V

A

R

G

E

S

D

D

R

H

T

T

V

V

T

K

G

A

L

I

L

V

I

I

T

C

S

G

G

A

K

N

D

G

S

N

F

F

I

C

V
x
A

G
|
G

A
|
A

D
|
D

I
|
I

F

H

E

G

F

F

T

S

D

A

L

F

-

T

-

P

-

G

-

L

-

A

F

L

G
|
G

M

S

A

L

E

V

D

D

E

E

I

I

A

Q

I

R

H

Y

N

Q

A

K

G

-

Q

-

S

-

A

-

V

-

I

-

T

-

A

-

L

-

A

-

D

-

L

-

P

-

V

-

P

P

T

V

V

L

V

A

A

A

V

I

N

Q

G

G

L
x
V

A

A

L
|
L

G

G

G
|
G

G

G

L

L

E
|
E

L

L

A

A

L

L

A

G

A

C

D

H

V

Y

R

R

M

I

L

A

S

N

D

A

S

K

A

A

R

R

I

V

G

G

L

L

P
|
P

E
x
A

V

V

G

T

L

L

G

G

I

I

F

L

P
|
P

G
|
G

F

A

G

R

G

G

T

T

V

Q

R

L

L

L

P

P

R

R

L

V

I

V

G

G

V

V

E

P

A

V

A

A

L

L

Q

D

W

L

I

I

M

T

S

S

G

G

T

K

P
x
Y

R

L

K

S

A

A

D

D

D

E

A

A

L

L

R

R

A

L

G

G

A

I

V

L

D

D

A

A

V

V

V

V

P

K

P

S

P

D

E

P

L

V

R

E

E

E

A

A

A

-

L

I

E

K

R

F

L

A

H

Q

G

K

L

I

V

I

A

D

S

K

P

P

D

-

W

-

S

I

E

E

R

P

R

R

K

R

E

I

A

F

L

N

S

K

P

P

V

V

V

P

R

S

A

L

G

-

E

P

D

N

L

M

A

D

P

S

I

V

F

F

A

A

A

E

A

A

K

I

A

A

K

K

A

V

R

R

K

K

T

Q

G

Y

P

P

H

G

L

V

P

L

A

A

S

P

L

E

A

T

V

C

V

V

E

R

L

S

I

I

E

Q

S

A

A

S

A

V

G

K

V

H

D

P

R

Y

D

E

A

V

A

G

L

I

A

K

L

E

E

E

A

E

R

K

A

L

F

F

A

M

H

Y

I

L

A

R

R

A

T

S

P

G

A

Q

A

A

R

K

A

A

L

L

V

Q

A

Y

V

A

F

F

I

F

N

A

D

E

Q
x
K

A

S

V

A

K

N

K

K

T

W

T

S

-

T

-

P

-

S

-

G

-

A

K

S

Q

W

Q

K

T

T

R

A

A

S

A

A

R

Q

P

P

V

V

S

S

R

S

A

V

A

G

V

V

L

L

G

G

A

L

G

G

I

T

M

M

G

G

G

R

G

G

I

I

A

A

Y

I

Q

S

S

F

A

A

V

R

C

V

G

G

I

I

P

S

V

V

I

V

M

A

K

V

D

E

I

S

A

D

Q

P

S

K

A

Q

L

L

D

D

L

A

G

-

M

-

T

-

Q

-

A

-

G

-

A

-

L

-

L

-

A

A

K

K

R

K

V

I

E

I

T

T

G

F

A

T

L

L

-

E

-

K

-

E

-

A

S

S

S

R

A

A

K

H

A

Q

D

N

G

G

I

-

L

Q

A

A

S

S

I

A

R

K

P

P

G

K

L

L

T

R

Y

F

D

S

G

S

-

S

-

T

-

K

-

E

I

L

A

S

E

T

A

V

D

D

V

L

I

V

V

V

E

E

A

A

V

V

V

F

E

E

N

D

I

M

A

N

I

L

K

K

R

K

A

K

V

V

L

F

A

A

E

E

L

L

E

S

E

A

H

L

I

C

R

K

P

P

D

G

A

A

V

F

V

L

A

C

T

T

N

N

T

T

S
|
S

S

A

L

L

L

N

V

V

A

D

D

D

L

I

A

A

R

S

D

S

L

T

E

D

W

R

P

P

E

Q

N

L

F

V

V

I

G

G

M

T

H
|
H

F

F

F

F

N

S

P

P

V

A

H

H

R

V

M

M

P

R

L

L

V

L

E
|
E

V

V

I

I

Q

P

G

S

P

R

H

Y

T

S

S

S

P

P

E

T

A

T

V

I

A

A

T

T

I

V

V

M

G

S

Y

L

A

S

S

K

A

K

M

I

G

G

K

K

T

I

P

G

V

V

V

V

V

V

R

G

D

N

C

C

P

Y

G

G

F

F

L

V

V

G

N
|
N

R

R

I

M

L

L

A

A

A

P

N

Y

V

Y

L

N

G

Q

F

G

L

F

R

F

L

L

I

L

H

E

D

E

G

G

A

S

D

K

F

P

L

E

R

D

I

V

D

D

A

G

A

V

M

L

E

E

G

E

F

F

G

G

W

F

P

K

M

M

G

G

P

P

A

F

W

R

L

V

Q

S

D

D

V

L

I

A

G

G

M

L

D

D

T

V

S

G

R

W

H

K

V

I

I

R

E

K

-

G

-

Q

-

G

-

L

-

T

-

G

-

P

V

S

I

L

A

P

A

P

G

G

Y

T

P

P

E

V

R

R

M

K

R

R

-

G

-

N

V

S

E

R

F

Y

A

S

T

P

A

L

L

G

D

D

V

M

M

L

V

C

E

E

S

A

K

G

R

R

F

F

G

G

Q

Q

K

K

S

T

G

G

A

K

G

G

F

W

Y

Y

R

Q

Y

Y

E

D

A

K

D

P

A

L

A

G

G

R

H

I

Q

H

R

K

K

P

V

-

V

-

D

D

P

P

D

W

V

L

G

S

A

T

L

F

L

L

A

S

T

Q

-

Y

-

R

-

E

V

V

Q

H

P

H

G

I

G

E

S

Q

R

R

I

T

I

I

S

S

D

K

E

E

E

E

I

I

V

L

Q

E

R

R

T

C

M

L

L

Y

P

S

M

L

I

I

L

N

E

E

T

A

A

F

R

R

C

I

L

L

E

E

D

E

G

G

V

M

V

A

G

A

S

R

A

P

A

E

E

H

A

I

D

D

M

V

A

I

L

Y

I

L

L

H

G

G

I

Y

G

G

F

W

P

P

R

R

H

H

L

K

G

G

G

G

A

P

L

M

H

F

Y

Y

A

A

D

A

E

S

L

V

G

G

A

L

K

P

T

T

I

V

M

L

E

E

W

K

C

L

D

Q

R

K

Y

Y

A

Y

N

R

L

Q

G

N

-

P

-

D

-

I

P

P

L

Q

Y

L

H

E

P

P

T

S

P

D

R

Y

M

L

R

R

R

R

L

L

A

V

V

A

D

Q

G

G

A

S

active site: A66 (= A68), G81 (= G78), G105 (= G116), E108 (= E119), P127 (= P138), A128 (≠ E139), P135 (= P146), G136 (= G147), S415 (= S427), H436 (= H448), E448 (= E460), N486 (= N498)
binding (2E)-Hexenoyl-CoA: V26 (= V28), A28 (≠ K30), A64 (≠ V66), G65 (= G67), A66 (= A68), D67 (= D69), I68 (= I70), V101 (≠ L112), L103 (= L114), G105 (= G116), E108 (= E119), G136 (= G147), Y161 (≠ P172), K280 (≠ Q295)

Sites not aligning to the query:

binding (2E)-Hexenoyl-CoA: 25

7o4uA Structure of the alpha subunit of mycobacterium tuberculosis beta- oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide (see paper)
33% identity, 93% coverage: 5:672/721 of query aligns to 2:673/711 of 7o4uA

query
sites
7o4uA

N

N

T

T

L

I

Q

Q

L

W

H

D

A

K

L

D

G

A

D

D

G

G

L

I

A

V

E

T

L

L

R

T

F

M

D

D

R

D

A

P

G

S

E

G

S

S

V

T

N

N

K

V

L

M

D

N

R

E

L

A

A

Y

L

I

D

E

E

S

L

M

N

G

R

K

A

A

L

V

C

D

-

R

L

L

V

V

R

A

E

E

D

K

R

D

T

S

V

I

T

T

G

G

L

V

L

V

I

V

T

A

S

S

G

A

K

K

D

K

S

T

F

F

I

F

V

A

G

G

A

G

D

D

I

V

F

K

E

-

F

-

T

T

D

M

L

I

F

Q

G

A

M

R

A

P

E

E

D

D

E

A

I

G

A

D

I

V

H

F

N

N

A

T

G

V

Q

E

S

T

A

I

V

K

-

R

-

Q

I

L

T

R

A

T

L

L

A

E

D

T

L

L

P

G

V

K

P

P

T

V

V

V

V

A

A

A

V

I

N

N

G

G

L

A

A

A

L

L

G

G

G

G

G

G

L

L

E

E

L

I

A

A

L

L

A

A

A

C

D

H

V

H

R

R

M

I

L

A

S

A

D

D

-

V

-

K

S

G

A

S

R

Q

I

L

G

G

L

L

P

P

E

E

V

V

G

T

L

L

G

G

I

L

F

L

P

P

G

G

F

G

G

G

G

G

T

V

V

T

R

R

L

T

P

V

R

R

L

M

I

F

G

G

V

I

E

Q

A

N

A

A

L

F

Q

V

W

S

I

V

M

L

S

A

-

Q

G

G

T

T

P

R

R

F

K

K

A

P

D

A

D

K

A

A

L

K

R

E

A

I

G

G

A

L

V

V

D

D

A

E

V

L

V

V

P

A

P

T

-

V

P

E

E

E

L

L

R

V

E

P

A

A

A

A

L

K

E

A

R

W

L

I

-

K

H

E

G

E

L

L

V

K

A

A

S

N

P

P

D

D

-

G

-

A

-

G

-

V

-

Q

-

P

W

W

S

D

E

K

R

K

R

G

K

Y

E

K

A

-

L

-

S

-

P

-

V

-

V

M

R

P

A

G

G

G

E

T

D

P

L

I

A

L

P

P

I

S

F

F

A

P

A

S

A

N

K

L

A

R

K

K

A

Q

R

L

K

K

T

G

G

A

P

P

H

-

L

M

P

P

A

A

S

P

L

R

A

A

V

I

V

L

E

A

L

A

I

A

E

V

S

E

A

G

A

A

G

Q

V

V

D

D

R

F

D

D

A

T

A

A

L

S

A

R

L

I

E

E

A

S

R

R

A

Y

F

F

A

A

H

S

I

L

A

V

R

T

T

G

P

Q

A

V

A

A

R

K

A

N

L

M

V

M

A

Q

V

A

F

F

I

F

N

F

D

D

-

L

Q

Q

A

A

V

I

K

N

K

A

T

G

-

G

T

S

K

R

Q

P

Q

E

T

G

R

I

A

G

A

K

R

T

P

P

V

I

S

K

R

R

A

I

A

G

V

V

L

L

G

G

A
|
A

G

G

I
x
M

M
|
M

G

G

G

A

G

G

I

I

A

A

Y

Y

Q

V

S

S

A

A

V

K

C

A

G

G

I

Y

P

E

V

V

I

V

M

L

K

K

D
|
D

I
x
V

A

S

Q

L

S

E

A

A

L

A

D

A

L

K

G

G

M

K

T

G

Q

Y

A

S

G

E

A

K

L

L

L

E

A

A

K

K

R

A

V

L

E

E

T

R

G

G

A

R

L

T

S

T

S

Q

A

E

K

R

A

S

D

D

G

A

I

L

L

L

A

A

S

R

I

I

R

T

P

P

G

T

L

A

T

D

Y

A

D

A

G

D

I

F

A

K

E

G

A

V

D

D

V

F

I

V

V

I

E

E

A

A

V
|
V

V
x
F

E
|
E

N

N

I

Q

A

E

I

L

K

K

R

H

A

K

V

V

L

F

A

G

E

E

L

I

E

E

E

D

H

I

I

V

R

E

P

P

D

N

A

A

V

I

V

L

A

G

T

S

N
|
N

T

T

S

S

S

T

L

L

L

P

V

I

A

T

D

G

L

L

A

A

R

T

D

G

L

V

E

K

W

R

P

Q

E

E

N

D

F

F

V

I

G

G

M

I

H
|
H

F

F

F

F

N

S

P

P

V

V

H

D

R

K

M

M

P

P

L

L

V

V

E

E

V

I

I

I

Q

K

G

G

P

E

H

K

T

T

S

S

P

D

E

E

A

A

V

L

A

A

T

R

I

V

V

F

G

D

Y

Y

A

T

S

L

A

A

M

I

G

G

K

K

T

T

P

P

V

I

V

V

V

V

R

N

D

D

C

S

P

R

G

G

F

F

L

F

V

T

N

S

R

R

I

V

L

I

A

G

A

T

N

F

V

V

L

N

G

E

F

A

L

L

R

A

L

M

I

L

H

G

D

E

G

G

A

V

D

E

F

P

L

A

R

S

I

I

D

E

A

Q

A

A

M

G

E

S

G

Q

F

A

G

G

W

Y

P

P

M

A

G

P

P

P

A

L

W

Q

L

L

Q

S

D

D

V

E

I

L

G

N

M

L

D

E

-

L

-

M

-

H

-

K

-

I

-

A

-

V

T

A

S

T

R

R

H

K

V

G

I

V

E

E

V

D

I

A

A

G

A

G

G

T

Y

Y

-

Q

-

P

-

H

P

P

E

A

R

E

M

A

R

V

V

V

E

E

F

-

A

-

T

-

A

-

L

-

D

-

V

K

M

M

V

I

E

E

S

L

K

G

R

R

F

S

G

G

Q

R

K

L

S

K

G

G

A

A

G

G

F

F

Y

Y

R

E

Y

Y

E

-

A

-

D

-

A

-

A

A

G

D

H

G

Q

K

R

R

K

S

V

G

V

L

D

W

P

P

D

-

V

-

G

-

A

G

L

L

L

R

A

E

T

T

V

F

Q

K

P

S

G

G

G

S

S

S

R

Q

I

-

I

P

S

P

D

L

E

Q

E

D

I

M

V

I

Q

D

R

R

T

M

M

L

L

F

P

A

M

E

I

A

L

L

E

E

T

T

A

Q

R

K

C

C

L

L

E

D

D

E

G

G

V

V

V

L

G

T

S

S

A

T

A

A

E

D

A

A

D

N

M

I

A

G

L

S

I

I

L

M

G

G

I

I

G

G

F

F

P

P

R

P

H

W

L

T

G

G

G

G

A

S

L

A

H

Q

Y

F

binding nicotinamide-adenine-dinucleotide: A324 (= A319), M326 (≠ I321), M327 (= M322), D346 (= D341), V347 (≠ I342), V403 (= V398), F404 (≠ V399), E405 (= E400), N430 (= N425), H453 (= H448)

Query Sequence

>WP_148219526.1 NCBI__GCF_000010725.1:WP_148219526.1
MFQGNTLQLHALGDGLAELRFDRAGESVNKLDRLALDELNRALCLVREDRTVTGLLITSG
KDSFIVGADIFEFTDLFGMAEDEIAIHNAGQSAVITALADLPVPTVVAVNGLALGGGLEL
ALAADVRMLSDSARIGLPEVGLGIFPGFGGTVRLPRLIGVEAALQWIMSGTPRKADDALR
AGAVDAVVPPPELREAALERLHGLVASPDWSERRKEALSPVVRAGEDLAPIFAAAKAKAR
KTGPHLPASLAVVELIESAAGVDRDAALALEARAFAHIARTPAARALVAVFINDQAVKKT
TKQQTRAARPVSRAAVLGAGIMGGGIAYQSAVCGIPVIMKDIAQSALDLGMTQAGALLAK
RVETGALSSAKADGILASIRPGLTYDGIAEADVIVEAVVENIAIKRAVLAELEEHIRPDA
VVATNTSSLLVADLARDLEWPENFVGMHFFNPVHRMPLVEVIQGPHTSPEAVATIVGYAS
AMGKTPVVVRDCPGFLVNRILAANVLGFLRLIHDGADFLRIDAAMEGFGWPMGPAWLQDV
IGMDTSRHVIEVIAAGYPERMRVEFATALDVMVESKRFGQKSGAGFYRYEADAAGHQRKV
VDPDVGALLATVQPGGSRIISDEEIVQRTMLPMILETARCLEDGVVGSAAEADMALILGI
GFPRHLGGALHYADELGAKTIMEWCDRYANLGPLYHPTPRMRRLAVDGAGWANLVQRPGA
L

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory