SitesBLAST
Comparing WP_150119821.1 NCBI__GCF_001584185.1:WP_150119821.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 9 hits to proteins with known functional sites (download)
8jejC Cryo-em structure of na-dithionite reduced membrane-bound fructose dehydrogenase from gluconobacter japonicus
43% identity, 82% coverage: 56:458/489 of query aligns to 1:411/413 of 8jejC
- binding heme c: C13 (= C68), C16 (= C71), H17 (= H72), T42 (≠ P96), I44 (= I98), F60 (= F114), L64 (= L118), L75 (= L129), Y76 (= Y130), M79 (≠ F133), P80 (= P134), Y84 (≠ F138), R122 (= R175), C162 (= C214), C165 (= C217), H166 (= H218), I186 (≠ L238), W189 (= W241), A191 (= A243), P192 (≠ F244), I194 (= I246), W205 (= W257), Y213 (= Y265), R223 (≠ S276), M228 (= M281), V303 (≠ N354), C304 (= C355), C307 (= C358), H308 (= H359), Y320 (= Y371), P321 (= P372), L323 (= L374), T327 (≠ S378), T328 (≠ V379), D336 (≠ N387), I341 (= I392), V345 (= V396), R347 (= R398), I354 (≠ T405), M356 (= M407), F359 (= F410), I376 (≠ L422)
- binding ubiquinone-10: M36 (≠ F90), P77 (= P131), S124 (≠ L177), W128 (= W181), C165 (= C217), L173 (≠ M225), L408 (= L455), L411 (= L458)
7w2jC Cryo-em structure of membrane-bound fructose dehydrogenase from gluconobacter japonicus
42% identity, 83% coverage: 56:463/489 of query aligns to 1:402/418 of 7w2jC
- binding heme c: C13 (= C68), C16 (= C71), H17 (= H72), T42 (≠ P96), I44 (= I98), Y55 (≠ W109), L75 (= L129), Y76 (= Y130), A78 (= A132), M79 (≠ F133), R122 (= R175), H161 (= H213), C162 (= C214), C165 (= C217), H166 (= H218), A191 (= A243), P192 (≠ F244), R223 (≠ S276), P227 (≠ G280), M228 (= M281), V289 (≠ N354), C290 (= C355), C293 (= C358), H294 (= H359), Y305 (= Y370), Y306 (= Y371), P307 (= P372), L309 (= L374), N312 (= N377), T313 (≠ S378), T314 (≠ V379), D322 (≠ N387), I327 (= I392), V331 (= V396), R333 (= R398), I340 (≠ T405), M342 (= M407), P343 (= P408), F345 (= F410)
Q47945 Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
37% identity, 86% coverage: 34:453/489 of query aligns to 20:441/478 of Q47945
- Q37 (≠ P51) modified: Pyrrolidone carboxylic acid
Sites not aligning to the query:
8gy2B Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
36% identity, 81% coverage: 56:453/489 of query aligns to 6:403/433 of 8gy2B
- binding heme c: C18 (= C68), C21 (= C71), H22 (= H72), T46 (≠ P96), I48 (= I98), Y59 (≠ W109), L68 (= L118), R73 (≠ D123), V79 (≠ L129), Y80 (= Y130), M83 (≠ F133), F88 (= F138), R126 (= R175), H165 (= H213), C166 (= C214), C169 (= C217), H170 (= H218), I201 (≠ T242), A202 (= A243), P203 (≠ F244), L205 (≠ I246), W216 (= W257), F224 (≠ Y265), A234 (≠ S276), V235 (≠ A277), F236 (≠ A278), F236 (≠ A278), M239 (= M281), N301 (= N354), C302 (= C355), C305 (= C358), H306 (= H359), M316 (≠ Y370), F317 (≠ Y371), P318 (= P372), L320 (= L374), P324 (≠ S378), G342 (vs. gap), S352 (≠ E403), V354 (≠ T405), M356 (= M407), F359 (= F410), M375 (≠ V426)
- binding ubiquinone-10: C21 (= C71), L34 (≠ Y84), P39 (= P89), P81 (= P131), L129 (≠ M178), W132 (= W181), E168 (= E216), R173 (= R221), I197 (≠ L238), D241 (≠ E283)
Sites not aligning to the query:
8gy3A Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
36% identity, 84% coverage: 52:461/489 of query aligns to 37:436/440 of 8gy3A
- binding heme c: Y52 (≠ D67), C53 (= C68), C56 (= C71), H57 (= H72), S84 (≠ P96), I86 (= I98), W97 (= W109), F102 (= F114), L117 (= L129), F121 (= F133), F126 (= F138), R163 (= R175), C203 (= C214), C206 (= C217), H207 (= H218), A232 (= A243), P233 (≠ F244), L235 (≠ I246), W245 (= W257), Y253 (= Y265), L254 (= L266), G263 (≠ A275), S264 (= S276), M269 (= M281), Y292 (≠ L304), C337 (= C355), C340 (= C358), H341 (= H359), P353 (= P372), L355 (= L374), N358 (= N377), N359 (≠ S378), V372 (= V391), I377 (≠ V396), G382 (≠ A401), Q383 (≠ K402), I386 (≠ T405), M388 (= M407), F391 (= F410)
- binding ubiquinone-10: E55 (≠ A70), T76 (= T88), F78 (= F90), Y118 (= Y130), P119 (= P131), I160 (≠ L172), G166 (≠ M178), Q167 (≠ A179), F169 (vs. gap), W170 (= W181), H202 (= H213), R210 (= R221), L213 (≠ T224)
8hddB Complex structure of catalytic, small, and a partial electron transfer subunits from burkholderia cepacia fad glucose dehydrogenase (see paper)
52% identity, 22% coverage: 323:432/489 of query aligns to 1:107/121 of 8hddB
- binding protoporphyrin ix containing fe: C30 (= C355), C33 (= C358), H34 (= H359), Y46 (= Y371), P47 (= P372), T54 (≠ V379), V66 (= V391), I67 (= I392), R73 (= R398), I80 (≠ T405), M82 (= M407), P83 (= P408)
4ax3D Structure of three-domain heme-cu nitrite reductase from ralstonia pickettii at 1.6 a resolution (see paper)
28% identity, 23% coverage: 337:447/489 of query aligns to 345:455/457 of 4ax3D
- binding heme c: C363 (= C355), C366 (= C358), H367 (= H359), P379 (= P372), P380 (≠ S373), L381 (= L374), S384 (≠ N377), F386 (≠ V379), N403 (vs. gap), G404 (vs. gap), S415 (≠ T405), M417 (= M407), M420 (≠ F410)
Sites not aligning to the query:
- active site: 93, 96, 98, 133, 134, 142, 147, 239, 261, 262, 288
- binding copper (ii) ion: 93, 98, 133, 134, 142, 147
6fjaA Crystal structure of t2d three-domain heme-cu nitrite reductase from ralstonia pickettii
28% identity, 23% coverage: 337:447/489 of query aligns to 342:452/455 of 6fjaA
- binding protoporphyrin ix containing fe: T359 (≠ N354), C360 (= C355), C363 (= C358), H364 (= H359), P376 (= P372), P377 (≠ S373), L378 (= L374), F383 (≠ V379), N400 (vs. gap), G401 (vs. gap), Y410 (≠ E403), S412 (≠ T405), M414 (= M407), M417 (≠ F410)
Sites not aligning to the query:
- active site: 90, 93, 95, 130, 131, 139, 144, 236, 258, 259, 285
- binding copper (ii) ion: 90, 131, 139, 144
5oboA Crystal structure of nitrite bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
28% identity, 23% coverage: 337:447/489 of query aligns to 343:453/456 of 5oboA
- binding heme c: T360 (≠ N354), C361 (= C355), C364 (= C358), H365 (= H359), P377 (= P372), P378 (≠ S373), L379 (= L374), S382 (≠ N377), F384 (≠ V379), I395 (≠ V391), N401 (vs. gap), G402 (vs. gap), S413 (≠ T405), M415 (= M407), M418 (≠ F410)
Sites not aligning to the query:
- active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
- binding copper (ii) ion: 91, 96, 131, 132, 140, 145
- binding nitrite ion: 94, 96, 131
Query Sequence
>WP_150119821.1 NCBI__GCF_001584185.1:WP_150119821.1
MHADDRRPVAAHRRHLEDSAMRARSQISAWCGGLALAAGLVHGAFAADAAPNADQIKRGE
YLAKAADCIACHTVDPAKPFAGGYPLATPFGTIYGPNITADKETGIGDWSDEQFVRALHE
GIDDEGKRLYPAFPYASFTKLSRDDVLAIKAYLFSLPPIQQKTPENKLPFPLNQRWLMAG
WNLFNFTPGELKADTAKSPEWNRGNYLVNGLAHCQECHTPRNLTMGLDLKRSFGGAQLGG
WTAFNISPDAVSGVGGWKDEELVQYLKTGVVPGKASAAGGMAEAIEHSLQYLTDDDLKAI
VTYLRSVPAVNDVADKKPRYAWGQPADDDAEIRGIAAVSVSSNASGGAELFSGNCASCHS
ASGSGVVGGYYPSLFNNSVVGARDPGNLLMVILNGVQRRGAKEETFMPGFAGHLNDGQIA
MLANYVVKQYGHADTPPITPEQVKVQRQGGPASPLLTLLPVGLAAAALIVVFILFSVFRR
GGRRQPKPS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory