SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_152568465.1 NCBI__GCF_000763315.1:WP_152568465.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
27% identity, 91% coverage: 33:439/447 of query aligns to 46:462/467 of 7qh2C

query
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7qh2C

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binding flavin-adenine dinucleotide: V73 (≠ P60), G75 (= G62), S76 (≠ L63), G77 (= G64), T78 (≠ A65), G79 (≠ A67), L80 (≠ S68), A83 (≠ H71), C84 (≠ P72), P137 (= P125), G138 (= G126), E139 (≠ W127), A142 (≠ L130), T143 (≠ S131), G146 (= G134), N147 (≠ G135), S149 (≠ V137), T150 (≠ A138), A152 (= A140), G153 (≠ T142), E203 (≠ L192), G204 (= G193), I209 (= I198), E422 (≠ L399), H423 (= H400)
binding fe (iii) ion: H377 (≠ G358), H384 (≠ T364), E422 (≠ L399)

8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
26% identity, 81% coverage: 76:439/447 of query aligns to 83:452/454 of 8jdzA

query
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8jdzA

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binding (3S)-3-methyl-2-oxopentanoic acid: R318 (≠ L296), W322 (= W300), H369 (= H356), H376 (≠ I363), H413 (= H400)
binding flavin-adenine dinucleotide: L90 (= L83), P132 (= P125), G133 (= G126), A134 (≠ W127), G140 (= G135), M141 (≠ I136), A143 (= A138), T144 (≠ A139), A146 (≠ L141), S147 (≠ T142), E200 (≠ L192), G201 (= G193), I206 (= I198), W322 (= W300), E412 (≠ L399), H413 (= H400), N449 (= N436)
binding manganese (ii) ion: H369 (= H356), H376 (≠ I363), E412 (≠ L399)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79

8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
26% identity, 81% coverage: 76:439/447 of query aligns to 83:452/454 of 8jdyA

query
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K

binding 2-oxo-4-methylpentanoic acid: R318 (≠ L296), W322 (= W300), S336 (= S320), H369 (= H356), H376 (≠ I363), H413 (= H400)
binding flavin-adenine dinucleotide: L90 (= L83), P132 (= P125), G133 (= G126), A134 (≠ W127), G140 (= G135), M141 (≠ I136), A143 (= A138), T144 (≠ A139), A146 (≠ L141), S147 (≠ T142), E200 (≠ L192), G201 (= G193), I206 (= I198), E412 (≠ L399), N449 (= N436)
binding manganese (ii) ion: H369 (= H356), H376 (≠ I363), E412 (≠ L399)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79

8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
26% identity, 81% coverage: 76:439/447 of query aligns to 83:454/456 of 8jdrA

query
sites
8jdrA

E

Q

A

G

V

V

T

C

L

I

S

N

L
|
L

A

T

R

H

Y

M

N

D

Q

Q

I

I

A

T

E

E

Y

L

S

N

A

T

G

E

D

D

L

F

M

S

V

V

T

V

V

V

Q

E

A

P

G

G

I

V

P

T

L

R

S

K

R

A

I

L

Q

N

S

T

V

H

L

L

R

R

E

D

K

S

G

G

Q

L

M

W

L

F

P

P

L

V

D

D

P
|
P

G
|
G

W
x
A

P

D

E

-

L

-

S

A

T

S

A

L

G

C

G
|
G

I
x
M

V

A

A
|
A

A
x
T

A

G

L
x
A

T
x
S

G

G

P

T

K

N

R

A

L

V

K

R

Y

Y

G

G

T

T

P

M

R

R

D

D

W

N

V

V

T

I

G

N

L

L

K

E

V

V

I

V

L

L

A

P

D

D

G

G

E

R

I

L

S

H

T

T

G

A

G

G

K

R

-

G

-

R

-

H

V

Y

V

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

M

L

N

T

K

G

L

L

F

F

V

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

V

I

I
|
I

S

T

E

S

C

T

T

T

F

L

K

R

L

L

R

H

P

P

L

A

P

P

P

E

E

A

E

T

T

V

V

A

I

A

I

T

L

C

E

A

N

F

G

P

D

S

L

V

P

Q

S

A

L

A

K

V

R

D

F

S

S

T

R

V

R

Q

L

I

L

L

D

Q

S

A

L

V

E

P

P

V

S

A

A

R

A

I

E

E

L

F

V

L

N

D

D

D

S

V

V

M

L

M

T

D

S

A

L

C

L

-

P

-

G

N

E

R

H

H

D

S

S

K

F

L

G

N

-

C

-

P

-

V

-

A

-

P

-

T

L

L

M

F

I

L

G

E

F

F

E

H

D

G

E

S

S

Q

K

Q

A

T

V

L

A

A

K

E

E

Q

Q

L

N

Q

Q

R

V

T

-

E

-

A

-

I

-

T

-

Q

-

D

-

N

-

G

-

S

-

H

V

F

N

S

W

W

A

A

K

A

E

E

E

K

G

R

I

N

T

E

I

L

R

W

E

A

I

A

L
x
R

R

H

N

N

E

A

W
|
W

A
x
Y

A

A

L

L

W

A

R

L

S

S

L

P

G

G

E

S

L

-

P

-

H

-

A

-

R

-

D

K

A

A

Q

Y

S

S

A

T

E

D

K

-

V

V

A

C

L

V

K

P

I

I

S

S

T

R

L

L

P

P

D

-

Q

-

A

-

D

E

S

I

V

L

V

V

E

E

L

T

V

K

H

E

R

E

L

I

A

K

E

A

E

S

A

K

G

L

I

T

E

G

A

A

L

I

V

V

-

G

H
|
H

G

V

G

G

P

D

G

G

T

N

G

F

I

A

T

C

L

I

A

L

V

L

L

V

R

D

P

P

D

D

E

D

-

A

N

E

Q

E

W

Q

A

R

C

R

A

V

K

K

E

A

L

F

I

A

E

E

K

N

V

L

R

G

Q

R

R

R

I

A

E

L

E

A

G

L

F

G

G

G

Y

T

V

C

V

T

M

G

L
x
E

H
|
H

A

G

P

I

S

G

F

L

L

G

K

K

E

R

K

Q

I

L

P

L

V

Q

W

E

G

E

K

V

M

G

P

P

S

V

G

G

L

V

F

E

L

T

M

M

Q

R

K

Q

I

L

K

K

S

N

S

T

L

L

D

D

P

P

E

R

G

G

R

L

F

M

N
|
N

P

P

G

G

R

K

binding flavin-adenine dinucleotide: L90 (= L83), P132 (= P125), G133 (= G126), A134 (≠ W127), G140 (= G135), M141 (≠ I136), A143 (= A138), T144 (≠ A139), A146 (≠ L141), S147 (≠ T142), E200 (≠ L192), G201 (= G193), I206 (= I198), Y324 (≠ A301), H370 (= H356), E414 (≠ L399), N451 (= N436)
binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (≠ L296), W323 (= W300), H415 (= H400)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79

8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
26% identity, 81% coverage: 76:439/447 of query aligns to 83:454/456 of 8jdqA

query
sites
8jdqA

E

Q

A

G

V

V

T

C

L

I

S

N

L
|
L

A

T

R

H

Y

M

N

D

Q

Q

I

I

A

T

E

E

Y

L

S

N

A

T

G

E

D

D

L

F

M

S

V

V

T

V

V

V

Q

E

A

P

G

G

I

V

P

T

L

R

S

K

R

A

I

L

Q

N

S

T

V

H

L

L

R

R

E

D

K

S

G

G

Q

L

M

W

L

F

P

P

L

V

D

D

P
|
P

G
|
G

W
x
A

P

D

E

-

L

-

S

A

T

S

A

L

G

C

G
|
G

I
x
M

V

A

A
|
A

A
x
T

A

G

L
x
A

T
x
S

G

G

P

T

K

N

R

A

L

V

K

R

Y

Y

G

G

T

T

P

M

R

R

D

D

W

N

V

V

T

I

G

N

L

L

K

E

V

V

I

V

L

L

A

P

D

D

G

G

E

R

I

L

S

H

T

T

G

A

G

G

K

R

-

G

-

R

-

H

V

Y

V

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

M

L

N

T

K

G

L

L

F

F

V

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

V

I

I
|
I

S

T

E

S

C

T

T

T

F

L

K

R

L

L

R

H

P

P

L

A

P

P

P

E

E

A

E

T

T

V

V

A

I

A

I

T

L

C

E

A

N

F

G

P

D

S

L

V

P

Q

S

A

L

A

K

V

R

D

F

S

S

T

R

V

R

Q

L

I

L

L

D

Q

S

A

L

V

E

P

P

V

S

A

A

R

A

I

E

E

L

F

V

L

N

D

D

D

S

V

V

M

L

M

T

D

S

A

L

C

L

-

P

-

G

N

E

R

H

H

D

S

S

K

F

L

G

N

-

C

-

P

-

V

-

A

-

P

-

T

L

L

M

F

I

L

G

E

F

F

E

H

D

G

E

S

S

Q

K

Q

A

T

V

L

A

A

K

E

E

Q

Q

L

N

Q

Q

R

V

T

-

E

-

A

-

I

-

T

-

Q

-

D

-

N

-

G

-

S

-

H

V

F

N

S

W

W

A

A

K

A

E

E

E

K

G

R

I

N

T

E

I

L

R

W

E

A

I

A

L
x
R

R

H

N

N

E

A

W
|
W

A

Y

A

A

L

L

W

A

R

L

S

S

L

P

G

G

E

S

L

-

P

-

H

-

A

-

R

-

D

K

A

A

Q

Y

S

S

A

T

E

D

K

-

V

V

A

C

L

V

K

P

I

I

S

S

T

R

L

L

P

P

D

-

Q

-

A

-

D

E

S

I

V

L

V

V

E

E

L

T

V

K

H

E

R

E

L

I

A

K

E

A

E

S

A

K

G

L

I

T

E

G

A

A

L

I

V

V

-

G

H
|
H

G

V

G

G

P

D

G

G

T

N

G

F

I

A

T

C

L

I

A

L

V

L

L

V

R

D

P

P

D

D

E

D

-

A

N

E

Q

E

W

Q

A

R

C

R

A

V

K

K

E

A

L

F

I

A

E

E

K

N

V

L

R

G

Q

R

R

R

I

A

E

L

E

A

G

L

F

G

G

G

Y

T

V

C

V

T

M

G

L
x
E

H
|
H

A

G

P

I

S

G

F

L

L

G

K

K

E

R

K

Q

I

L

P

L

V

Q

W

E

G

E

K

V

M

G

P

P

S

V

G

G

L

V

F

E

L

T

M

M

Q

R

K

Q

I

L

K

K

S

N

S

T

L

L

D

D

P

P

E

R

G

G

R

L

F

M

N
|
N

P

P

G

G

R

K

binding (2R)-2-hydroxy-4-methylpentanoic acid: R319 (≠ L296), W323 (= W300), H370 (= H356), H415 (= H400)
binding flavin-adenine dinucleotide: L90 (= L83), P132 (= P125), G133 (= G126), A134 (≠ W127), G140 (= G135), M141 (≠ I136), A143 (= A138), T144 (≠ A139), A146 (≠ L141), S147 (≠ T142), E200 (≠ L192), G201 (= G193), I206 (= I198), H370 (= H356), E414 (≠ L399), N451 (= N436)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79

8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid (see paper)
26% identity, 81% coverage: 76:439/447 of query aligns to 83:454/456 of 8jdoA

query
sites
8jdoA

E

Q

A

G

V

V

T

C

L

I

S

N

L
|
L

A

T

R

H

Y

M

N

D

Q

Q

I

I

A

T

E

E

Y

L

S

N

A

T

G

E

D

D

L

F

M

S

V

V

T

V

V

V

Q

E

A

P

G

G

I

V

P

T

L

R

S

K

R

A

I

L

Q

N

S

T

V

H

L

L

R

R

E

D

K

S

G

G

Q

L

M

W

L

F

P

P

L

V

D

D

P
|
P

G
|
G

W
x
A

P

D

E

-

L

-

S

A

T

S

A

L

G

C

G
|
G

I
x
M

V

A

A
|
A

A
x
T

A

G

L
x
A

T
x
S

G

G

P

T

K

N

R

A

L

V

K

R

Y

Y

G

G

T

T

P

M

R

R

D

D

W

N

V

V

T

I

G

N

L

L

K

E

V

V

I

V

L

L

A

P

D

D

G

G

E

R

I

L

S

H

T

T

G

A

G

G

K

R

-

G

-

R

-

H

V

Y

V

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

M

L

N

T

K

G

L

L

F

F

V

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

V

I

I
|
I

S

T

E

S

C

T

T

T

F

L

K

R

L

L

R

H

P

P

L

A

P

P

P

E

E

A

E

T

T

V

V

A

I

A

I

T

L

C

E

A

N

F

G

P

D

S

L

V

P

Q

S

A

L

A

K

V

R

D

F

S

S

T

R

V

R

Q

L

I

L

L

D

Q

S

A

L

V

E

P

P

V

S

A

A

R

A

I

E

E

L

F

V

L

N

D

D

D

S

V

V

M

L

M

T

D

S

A

L

C

L

-

P

-

G

N

E

R

H

H

D

S

S

K

F

L

G

N

-

C

-

P

-

V

-

A

-

P

-

T

L

L

M

F

I

L

G

E

F

F

E

H

D

G

E

S

S

Q

K

Q

A

T

V

L

A

A

K

E

E

Q

Q

L

N

Q

Q

R

V

T

-

E

-

A

-

I

-

T

-

Q

-

D

-

N

-

G

-

S

-

H

V

F

N

S

W

W

A

A

K

A

E

E

E

K

G

R

I

N

T

E

I

L

R

W

E

A

I

A

L
x
R

R

H

N

N

E

A

W
|
W

A
x
Y

A

A

L

L

W

A

R

L

S

S

L

P

G

G

E

S

L

-

P

-

H

-

A

-

R

-

D

K

A

A

Q

Y

S

S

A

T

E

D

K

-

V

V

A

C

L

V

K

P

I

I

S

S

T

R

L

L

P

P

D

-

Q

-

A

-

D

E

S

I

V

L

V

V

E

E

L

T

V

K

H

E

R

E

L

I

A

K

E

A

E

S

A

K

G

L

I

T

E

G

A

A

L

I

V

V

-

G

H
|
H

G

V

G

G

P

D

G

G

T

N

G

F

I

A

T

C

L

I

A

L

V

L

L

V

R

D

P

P

D

D

E

D

-

A

N

E

Q

E

W

Q

A

R

C

R

A

V

K

K

E

A

L

F

I

A

E

E

K

N

V

L

R

G

Q

R

R

R

I

A

E

L

E

A

G

L

F

G

G

G

Y

T

V

C

V

T

M

G

L
x
E

H
|
H

A

G

P

I

S

G

F

L

L

G

K

K

E

R

K

Q

I

L

P

L

V

Q

W

E

G

E

K

V

M

G

P

P

S

V

G

G

L

V

F

E

L

T

M

M

Q

R

K

Q

I

L

K

K

S

N

S

T

L

L

D

D

P

P

E

R

G

G

R

L

F

M

N
|
N

P

P

G

G

R

K

binding (2R)-2-hydroxyhexanoic acid: R319 (≠ L296), W323 (= W300), H415 (= H400)
binding flavin-adenine dinucleotide: L90 (= L83), P132 (= P125), G133 (= G126), A134 (≠ W127), G140 (= G135), M141 (≠ I136), A143 (= A138), T144 (≠ A139), A146 (≠ L141), S147 (≠ T142), E200 (≠ L192), G201 (= G193), I206 (= I198), Y324 (≠ A301), H370 (= H356), E414 (≠ L399), N451 (= N436)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79

8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid (see paper)
26% identity, 81% coverage: 76:439/447 of query aligns to 83:454/456 of 8jdnA

query
sites
8jdnA

E

Q

A

G

V

V

T

C

L

I

S

N

L
|
L

A

T

R

H

Y

M

N

D

Q

Q

I

I

A

T

E

E

Y

L

S

N

A

T

G

E

D

D

L

F

M

S

V

V

T

V

V

V

Q

E

A

P

G

G

I

V

P

T

L

R

S

K

R

A

I

L

Q

N

S

T

V

H

L

L

R

R

E

D

K

S

G

G

Q

L

M

W

L

F

P

P

L

V

D

D

P
|
P

G
|
G

W
x
A

P

D

E

-

L

-

S

A

T

S

A

L

G

C

G
|
G

I
x
M

V

A

A
|
A

A
x
T

A

G

L
x
A

T
x
S

G

G

P

T

K

N

R

A

L

V

K

R

Y

Y

G

G

T

T

P

M

R

R

D

D

W

N

V

V

T

I

G

N

L

L

K

E

V

V

I

V

L

L

A

P

D

D

G

G

E

R

I

L

S

H

T

T

G

A

G

G

K

R

-

G

-

R

-

H

V

Y

V

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

M

L

N

T

K

G

L

L

F

F

V

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

V

I

I
|
I

S

T

E

S

C

T

T

T

F

L

K

R

L

L

R

H

P

P

L

A

P

P

P

E

E

A

E

T

T

V

V

A

I

A

I

T

L

C

E

A

N

F

G

P

D

S

L

V

P

Q

S

A

L

A

K

V

R

D

F

S

S

T

R

V

R

Q

L

I

L

L

D

Q

S

A

L

V

E

P

P

V

S

A

A

R

A

I

E

E

L

F

V

L

N

D

D

D

S

V

V

M

L

M

T

D

S

A

L

C

L

-

P

-

G

N

E

R

H

H

D

S

S

K

F

L

G

N

-

C

-

P

-

V

-

A

-

P

-

T

L

L

M

F

I

L

G

E

F

F

E

H

D

G

E

S

S

Q

K

Q

A

T

V

L

A

A

K

E

E

Q

Q

L

N

Q

Q

R

V

T

-

E

-

A

-

I

-

T

-

Q

-

D

-

N

-

G

-

S

-

H

V

F

N

S

W

W

A

A

K

A

E

E

E

K

G

R

I

N

T

E

I

L

R

W

E

A

I

A

L
x
R

R

H

N

N

E

A

W
|
W

A

Y

A

A

L

L

W

A

R

L

S

S

L

P

G

G

E

S

L

-

P

-

H

-

A

-

R

-

D

K

A

A

Q

Y

S

S

A

T

E

D

K

-

V

V

A

C

L

V

K

P

I

I

S

S

T

R

L

L

P

P

D

-

Q

-

A

-

D

E

S

I

V

L

V

V

E

E

L

T

V

K

H

E

R

E

L

I

A

K

E

A

E

S

A

K

G

L

I

T

E

G

A

A

L

I

V

V

-

G

H
|
H

G

V

G

G

P

D

G

G

T

N

G

F

I

A

T

C

L

I

A

L

V

L

L

V

R

D

P

P

D

D

E

D

-

A

N

E

Q

E

W

Q

A

R

C

R

A

V

K

K

E

A

L

F

I

A

E

E

K

N

V

L

R

G

Q

R

R

R

I

A

E

L

E

A

G

L

F

G

G

G

Y

T

V

C

V

T

M

G

L
x
E

H
|
H

A

G

P

I

S

G

F

L

L

G

K

K

E

R

K

Q

I

L

P

L

V

Q

W

E

G

E

K

V

M

G

P

P

S

V

G

G

L

V

F

E

L

T

M

M

Q

R

K

Q

I

L

K

K

S

N

S

T

L

L

D

D

P

P

E

R

G

G

R

L

F

M

N
|
N

P

P

G

G

R

K

binding flavin-adenine dinucleotide: L90 (= L83), P132 (= P125), G133 (= G126), A134 (≠ W127), G140 (= G135), M141 (≠ I136), A143 (= A138), T144 (≠ A139), A146 (≠ L141), S147 (≠ T142), E200 (≠ L192), G201 (= G193), I206 (= I198), H370 (= H356), E414 (≠ L399), N451 (= N436)
binding (2R)-2-oxidanylpentanoic acid: R319 (≠ L296), W323 (= W300), H415 (= H400)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79

8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid (see paper)
26% identity, 81% coverage: 76:439/447 of query aligns to 83:454/456 of 8jdgA

query
sites
8jdgA

E

Q

A

G

V

V

T

C

L

I

S

N

L
|
L

A

T

R

H

Y

M

N

D

Q

Q

I

I

A

T

E

E

Y

L

S

N

A

T

G

E

D

D

L

F

M

S

V

V

T

V

V

V

Q

E

A

P

G

G

I

V

P

T

L

R

S

K

R

A

I

L

Q

N

S

T

V

H

L

L

R

R

E

D

K

S

G

G

Q

L

M

W

L

F

P

P

L

V

D

D

P
|
P

G
|
G

W
x
A

P

D

E

-

L

-

S

A

T

S

A

L

G

C

G
|
G

I
x
M

V

A

A
|
A

A
x
T

A

G

L
x
A

T
x
S

G

G

P

T

K

N

R

A

L

V

K

R

Y

Y

G

G

T

T

P

M

R

R

D

D

W

N

V

V

T

I

G

N

L

L

K

E

V

V

I

V

L

L

A

P

D

D

G

G

E

R

I

L

S

H

T

T

G

A

G

G

K

R

-

G

-

R

-

H

V

Y

V

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

M

L

N

T

K

G

L

L

F

F

V

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

V

I

I
|
I

S

T

E

S

C

T

T

T

F

L

K

R

L

L

R

H

P

P

L

A

P

P

P

E

E

A

E

T

T

V

V

A

I

A

I

T

L

C

E

A

N

F

G

P

D

S

L

V

P

Q

S

A

L

A

K

V

R

D

F

S

S

T

R

V

R

Q

L

I

L

L

D

Q

S

A

L

V

E

P

P

V

S

A

A

R

A

I

E

E

L

F

V

L

N

D

D

D

S

V

V

M

L

M

T

D

S

A

L

C

L

-

P

-

G

N

E

R

H

H

D

S

S

K

F

L

G

N

-

C

-

P

-

V

-

A

-

P

-

T

L

L

M

F

I

L

G

E

F

F

E

H

D

G

E

S

S

Q

K

Q

A

T

V

L

A

A

K

E

E

Q

Q

L

N

Q

Q

R

V

T

-

E

-

A

-

I

-

T

-

Q

-

D

-

N

-

G

-

S

-

H

V

F

N

S

W

W

A

A

K

A

E

E

E

K

G

R

I

N

T

E

I

L

R

W

E

A

I

A

L
x
R

R

H

N

N

E

A

W

W

A

Y

A

A

L

L

W

A

R

L

S

S

L

P

G

G

E

S

L

-

P

-

H

-

A

-

R

-

D

K

A

A

Q

Y

S

S

A

T

E

D

K

-

V

V

A

C

L

V

K

P

I

I

S

S

T

R

L

L

P

P

D

-

Q

-

A

-

D

E

S

I

V

L

V

V

E

E

L

T

V

K

H

E

R

E

L

I

A

K

E

A

E

S

A

K

G

L

I

T

E

G

A

A

L

I

V

V

-

G

H
|
H

G

V

G

G

P

D

G

G

T

N

G

F

I

A

T

C

L

I

A

L

V

L

L

V

R

D

P

P

D

D

E

D

-

A

N

E

Q

E

W

Q

A

R

C

R

A

V

K

K

E

A

L

F

I

A

E

E

K

N

V

L

R

G

Q

R

R

R

I

A

E

L

E

A

G

L

F

G

G

G

Y

T

V

C

V

T

M

G

L
x
E

H
|
H

A

G

P

I

S

G

F

L

L

G

K

K

E

R

K

Q

I

L

P

L

V

Q

W

E

G

E

K

V

M

G

P

P

S

V

G

G

L

V

F

E

L

T

M

M

Q

R

K

Q

I

L

K

K

S

N

S

T

L

L

D

D

P

P

E

R

G

G

R

L

F

M

N
|
N

P

P

G

G

R

K

binding flavin-adenine dinucleotide: L90 (= L83), P132 (= P125), G133 (= G126), A134 (≠ W127), G140 (= G135), M141 (≠ I136), A143 (= A138), T144 (≠ A139), A146 (≠ L141), S147 (≠ T142), E200 (≠ L192), G201 (= G193), I206 (= I198), H370 (= H356), E414 (≠ L399), N451 (= N436)
binding (2R)-2-oxidanylbutanoic acid: R319 (≠ L296), H415 (= H400)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79

8jdbA Crystal structure of h405a mldhd in complex with d-2-hydroxyoctanoic acid (see paper)
26% identity, 81% coverage: 76:439/447 of query aligns to 83:454/456 of 8jdbA

query
sites
8jdbA

E

Q

A

G

V

V

T

C

L

I

S

N

L
|
L

A

T

R

H

Y

M

N

D

Q

Q

I

I

A

T

E

E

Y

L

S

N

A

T

G

E

D

D

L

F

M

S

V

V

T

V

V

V

Q

E

A

P

G

G

I

V

P

T

L

R

S

K

R

A

I

L

Q

N

S

T

V

H

L

L

R

R

E

D

K

S

G

G

Q

L

M

W

L

F

P

P

L

V

D

D

P
|
P

G
|
G

W
x
A

P

D

E

-

L

-

S

A

T

S

A

L

G

C

G
|
G

I
x
M

V

A

A
|
A

A
x
T

A

G

L
x
A

T
x
S

G

G

P

T

K

N

R

A

L

V

K

R

Y

Y

G

G

T

T

P

M

R

R

D

D

W

N

V

V

T

I

G

N

L

L

K

E

V

V

I

V

L

L

A

P

D

D

G

G

E

R

I

L

S

H

T

T

G

A

G

G

K

R

-

G

-

R

-

H

V

Y

V

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

M

L

N

T

K

G

L

L

F

F

V

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

V

I

I
|
I

S

T

E

S

C

T

T

T

F

L

K

R

L

L

R

H

P

P

L

A

P

P

P

E

E

A

E

T

T

V

V

A

I

A

I

T

L

C

E

A

N

F

G

P

D

S

L

V

P

Q

S

A

L

A

K

V

R

D

F

S

S

T

R

V

R

Q

L

I

L

L

D

Q

S

A

L

V

E

P

P

V

S

A

A

R

A

I

E

E

L

F

V

L

N

D

D

D

S

V

V

M

L

M

T

D

S

A

L

C

L

-

P

-

G

N

E

R

H

H

D

S

S

K

F

L

G

N

-

C

-

P

-

V

-

A

-

P

-

T

L

L

M

F

I

L

G

E

F

F

E

H

D

G

E

S

S

Q

K

Q

A

T

V

L

A

A

K

E

E

Q

Q

L

N

Q

Q

R

V

T

-

E

-

A

-

I

-

T

-

Q

-

D

-

N

-

G

-

S

-

H

V

F

N

S

W

W

A

A

K

A

E

E

E

K

G

R

I

N

T

E

I

L

R

W

E

A

I

A

L
x
R

R

H

N

N

E

A

W
|
W

A
x
Y

A

A

L

L

W

A

R

L

S

S

L

P

G

G

E

S

L

-

P

-

H

-

A

-

R

-

D

K

A

A

Q

Y

S

S

A

T

E

D

K

-

V

V

A

C

L

V

K

P

I

I

S

S

T

R

L

L

P

P

D

-

Q

-

A

-

D

E

S

I

V

L

V

V

E

E

L

T

V

K

H

E

R

E

L

I

A

K

E

A

E

S

A

K

G

L

I

T

E

G

A

A

L

I

V

V

-

G

H
|
H

G

V

G

G

P

D

G

G

T

N

G

F

I

A

T

C

L

I

A

L

V

L

L

V

R

D

P

P

D

D

E

D

-

A

N

E

Q

E

W

Q

A

R

C

R

A

V

K

K

E

A

L

F

I

A

E

E

K

N

V

L

R

G

Q

R

R

R

I

A

E

L

E

A

G

L

F

G

G

G

Y

T

V

C

V

T

M

G

L
x
E

H
|
H

A

G

P

I

S

G

F

L

L

G

K

K

E

R

K

Q

I

L

P

L

V

Q

W

E

G

E

K

V

M

G

P

P

S

V

G

G

L

V

F

E

L

T

M

M

Q

R

K

Q

I

L

K

K

S

N

S

T

L

L

D

D

P

P

E

R

G

G

R

L

F

M

N
|
N

P

P

G

G

R

K

binding flavin-adenine dinucleotide: L90 (= L83), P132 (= P125), G133 (= G126), A134 (≠ W127), G140 (= G135), M141 (≠ I136), A143 (= A138), T144 (≠ A139), A146 (≠ L141), S147 (≠ T142), E200 (≠ L192), G201 (= G193), I206 (= I198), Y324 (≠ A301), H370 (= H356), E414 (≠ L399), N451 (= N436)
binding (2R)-2-oxidanyloctanoic acid: R319 (≠ L296), W323 (= W300), H415 (= H400)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79
binding (2R)-2-oxidanyloctanoic acid: 75

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
26% identity, 81% coverage: 76:439/447 of query aligns to 83:453/455 of 8jduA

query
sites
8jduA

E

Q

A

G

V

V

T

C

L

I

S

N

L
|
L

A

T

R

H

Y

M

N

D

Q

Q

I

I

A

T

E

E

Y

L

S

N

A

T

G

E

D

D

L

F

M

S

V

V

T

V

V

V

Q

E

A

P

G

G

I

V

P

T

L

R

S

K

R

A

I

L

Q

N

S

T

V

H

L

L

R

R

E

D

K

S

G

G

Q

L

M

W

L

F

P

P

L

V

D

D

P
|
P

G
|
G

W
x
A

P

D

E

-

L

-

S

A

T

S

A

L

G

C

G
|
G

I
x
M

V

A

A
|
A

A
x
T

A

G

L
x
A

T
x
S

G

G

P

T

K

N

R

A

L

V

K

R

Y

Y

G

G

T

T

P

M

R

R

D

D

W

N

V

V

T

I

G

N

L

L

K

E

V

V

I

V

L

L

A

P

D

D

G

G

E

R

I

L

S

H

T

T

G

A

G

G

K

R

-

G

-

R

-

H

V

Y

V

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

M

L

N

T

K

G

L

L

F

F

V

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

V

I

I
|
I

S

T

E

S

C

T

T

T

F

L

K

R

L

L

R

H

P

P

L

A

P

P

P

E

E

A

E

T

T

V

V

A

I

A

I

T

L

C

E

A

N

F

G

P

D

S

L

V

P

Q

S

A

L

A

K

V

R

D

F

S

S

T

R

V

R

Q

L

I

L

L

D

Q

S

A

L

V

E

P

P

V

S

A

A

R

A

I

E

E

L

F

V

L

N

D

D

D

S

V

V

M

L

M

T

D

S

A

L

C

L

-

P

-

G

N

E

R

H

H

D

S

S

K

F

L

G

N

-

C

-

P

-

V

-

A

-

P

-

T

L

L

M

F

I

L

G

E

F

F

E

H

D

G

E

S

S

Q

K

Q

A

T

V

L

A

A

K

E

E

Q

Q

L

N

Q

Q

R

V

T

-

E

-

A

-

I

-

T

-

Q

-

N

-

G

-

S

-

H

V

F

N

S

W

W

A

A

K

A

E

E

E

K

G

R

I

N

T

E

I

L

R

W

E

A

I

A

L
x
R

R

H

N

N

E

A

W
|
W

A

Y

A

A

L

L

W

A

R

L

S

S

L

P

G

G

E

S

L

-

P

-

H

-

A

-

R

-

D

K

A

A

Q

Y

S

S

A

T

E

D

K

-

V

V

A

C

L

V

K

P

I

I

S

S

T

R

L

L

P

P

D

-

Q

-

A

-

D

E

S

I

V

L

V

V

E

E

L

T

V

K

H

E

R

E

L

I

A

K

E

A

E

S

A

K

G

L

I

T

E

G

A

A

L

I

V

V

-

G

H
|
H

G

V

G

G

P

D

G

G

T

N

G

F

I
x
H

T

C

L

I

A

L

V

L

L

V

R

D

P

P

D

D

E

D

-

A

N

E

Q

E

W

Q

A

R

C

R

A

V

K

K

E

A

L

F

I

A

E

E

K

N

V

L

R

G

Q

R

R

R

I

A

E

L

E

A

G

L

F

G

G

G

Y

T

V

C

V

T

M

G

L
x
E

H
|
H

A

G

P

I

S

G

F

L

L

G

K

K

E

R

K

Q

I

L

P

L

V

Q

W

E

G

E

K

V

M

G

P

P

S

V

G

G

L

V

F

E

L

T

M

M

Q

R

K

Q

I

L

K

K

S

N

S

T

L

L

D

D

P

P

E

R

G

G

R

L

F

M

N
|
N

P

P

G

G

R

K

binding 2-oxopentanoic acid: R318 (≠ L296), W322 (= W300), H369 (= H356), H376 (≠ I363), H414 (= H400)
binding flavin-adenine dinucleotide: L90 (= L83), P132 (= P125), G133 (= G126), A134 (≠ W127), G140 (= G135), M141 (≠ I136), A143 (= A138), T144 (≠ A139), A146 (≠ L141), S147 (≠ T142), E200 (≠ L192), G201 (= G193), I206 (= I198), W322 (= W300), E413 (≠ L399), N450 (= N436)
binding manganese (ii) ion: H369 (= H356), H376 (≠ I363), E413 (≠ L399)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
26% identity, 81% coverage: 76:439/447 of query aligns to 83:454/456 of 8jdsA

query
sites
8jdsA

E

Q

A

G

V

V

T

C

L

I

S

N

L
|
L

A

T

R

H

Y

M

N

D

Q

Q

I

I

A

T

E

E

Y

L

S

N

A

T

G

E

D

D

L

F

M

S

V

V

T

V

V

V

Q

E

A

P

G

G

I

V

P

T

L

R

S

K

R

A

I

L

Q

N

S

T

V

H

L

L

R

R

E

D

K

S

G

G

Q

L

M

W

L

F

P

P

L

V

D

D

P
|
P

G
|
G

W
x
A

P

D

E

-

L

-

S

A

T

S

A

L

G

C

G
|
G

I
x
M

V

A

A
|
A

A
x
T

A

G

L
x
A

T
x
S

G

G

P

T

K

N

R

A

L

V

K

R

Y

Y

G

G

T

T

P

M

R

R

D

D

W

N

V

V

T

I

G

N

L

L

K

E

V

V

I

V

L

L

A

P

D

D

G

G

E

R

I

L

S

H

T

T

G

A

G

G

K

R

-

G

-

R

-

H

V

Y

V

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

M

L

N

T

K

G

L

L

F

F

V

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

V

I

I
|
I

S

T

E

S

C

T

T

T

F

L

K

R

L

L

R

H

P

P

L

A

P

P

P

E

E

A

E

T

T

V

V

A

I

A

I

T

L

C

E

A

N

F

G

P

D

S

L

V

P

Q

S

A

L

A

K

V

R

D

F

S

S

T

R

V

R

Q

L

I

L

L

D

Q

S

A

L

V

E

P

P

V

S

A

A

R

A

I

E

E

L

F

V

L

N

D

D

D

S

V

V

M

L

M

T

D

S

A

L

C

L

-

P

-

G

N

E

R

H

H

D

S

S

K

F

L

G

N

-

C

-

P

-

V

-

A

-

P

-

T

L

L

M

F

I

L

G

E

F

F

E

H

D

G

E

S

S

Q

K

Q

A

T

V

L

A

A

K

E

E

Q

Q

L

N

Q

Q

R

V

T

-

E

-

A

-

I

-

T

-

Q

-

D

-

N

-

G

-

S

-

H

V

F

N

S

W

W

A

A

K

A

E

E

E

K

G

R

I

N

T

E

I

L

R

W

E

A

I

A

L
x
R

R

H

N

N

E

A

W
|
W

A

Y

A

A

L

L

W

A

R

L

S

S

L

P

G

G

E

S

L

-

P

-

H

-

A

-

R

-

D

K

A

A

Q

Y

S

S

A

T

E

D

K

-

V

V

A

C

L

V

K

P

I

I

S

S

T

R

L

L

P

P

D

-

Q

-

A

-

D

E

S

I

V

L

V

V

E

E

L

T

V

K

H

E

R

E

L

I

A

K

E

A

E

S

A

K

G

L

I

T

E

G

A

A

L

I

V

V

-

G

H
|
H

G

V

G

G

P

D

G

G

T

N

G

F

I
x
H

T

C

L

I

A

L

V

L

L

V

R

D

P

P

D

D

E

D

-

A

N

E

Q

E

W

Q

A

R

C

R

A

V

K

K

E

A

L

F

I

A

E

E

K

N

V

L

R

G

Q

R

R

R

I

A

E

L

E

A

G

L

F

G

G

G

Y

T

V

C

V

T

M

G

L
x
E

H
|
H

A

G

P

I

S

G

F

L

L

G

K

K

E

R

K

Q

I

L

P

L

V

Q

W

E

G

E

K

V

M

G

P

P

S

V

G

G

L

V

F

E

L

T

M

M

Q

R

K

Q

I

L

K

K

S

N

S

T

L

L

D

D

P

P

E

R

G

G

R

L

F

M

N
|
N

P

P

G

G

R

K

binding flavin-adenine dinucleotide: L90 (= L83), P132 (= P125), G133 (= G126), A134 (≠ W127), G140 (= G135), M141 (≠ I136), A143 (= A138), T144 (≠ A139), A146 (≠ L141), S147 (≠ T142), E200 (≠ L192), G201 (= G193), I206 (= I198), W323 (= W300), E414 (≠ L399), H415 (= H400), N451 (= N436)
binding manganese (ii) ion: H370 (= H356), H377 (≠ I363), E414 (≠ L399)
binding pyruvic acid: R319 (≠ L296), H370 (= H356), H377 (≠ I363), H415 (= H400)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79

8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
26% identity, 81% coverage: 76:439/447 of query aligns to 83:453/455 of 8jdxA

query
sites
8jdxA

E

Q

A

G

V

V

T

C

L

I

S

N

L
|
L

A

T

R

H

Y

M

N

D

Q

Q