SitesBLAST
Comparing WP_156424864.1 NCBI__GCF_001519075.1:WP_156424864.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
37% identity, 93% coverage: 21:277/277 of query aligns to 3:259/259 of 5zaiC
- active site: A65 (≠ S83), F70 (vs. gap), S82 (≠ P97), R86 (≠ Y101), G110 (= G125), E113 (= E128), P132 (= P147), E133 (= E148), I138 (≠ A153), P140 (≠ A155), G141 (≠ L156), A226 (≠ M241), F236 (≠ S254)
- binding coenzyme a: K24 (≠ V42), L25 (= L43), A63 (= A81), G64 (= G82), A65 (≠ S83), D66 (= D84), I67 (≠ L85), P132 (= P147), R166 (≠ P181), F248 (= F266), K251 (= K269)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
37% identity, 93% coverage: 20:277/277 of query aligns to 1:256/256 of 3h81A
- active site: A64 (≠ S83), M69 (≠ V88), T79 (≠ P97), F83 (≠ Y101), G107 (= G125), E110 (= E128), P129 (= P147), E130 (= E148), V135 (≠ A153), P137 (≠ A155), G138 (≠ L156), L223 (≠ A242), F233 (≠ S254)
- binding calcium ion: F233 (≠ S254), Q238 (≠ L259)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
38% identity, 92% coverage: 20:274/277 of query aligns to 2:254/255 of 3q0jC
- active site: A65 (≠ S83), M70 (≠ V88), T80 (≠ P97), F84 (≠ Y101), G108 (= G125), E111 (= E128), P130 (= P147), E131 (= E148), V136 (≠ A153), P138 (≠ A155), G139 (≠ L156), L224 (≠ A242), F234 (≠ S254)
- binding acetoacetyl-coenzyme a: Q23 (≠ A41), A24 (≠ V42), L25 (= L43), A27 (= A45), A63 (= A81), G64 (= G82), A65 (≠ S83), D66 (= D84), I67 (≠ L85), K68 (= K86), M70 (≠ V88), F84 (≠ Y101), G107 (= G124), G108 (= G125), E111 (= E128), P130 (= P147), E131 (= E148), P138 (≠ A155), G139 (≠ L156), M140 (≠ G157)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
38% identity, 92% coverage: 20:274/277 of query aligns to 2:254/255 of 3q0gC
- active site: A65 (≠ S83), M70 (≠ V88), T80 (≠ P97), F84 (≠ Y101), G108 (= G125), E111 (= E128), P130 (= P147), E131 (= E148), V136 (≠ A153), P138 (≠ A155), G139 (≠ L156), L224 (≠ A242), F234 (≠ S254)
- binding coenzyme a: L25 (= L43), A63 (= A81), I67 (≠ L85), K68 (= K86), Y104 (≠ L121), P130 (= P147), E131 (= E148), L134 (≠ V151)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
38% identity, 92% coverage: 20:274/277 of query aligns to 1:249/250 of 3q0gD
- active site: A64 (≠ S83), M69 (≠ V88), T75 (≠ P97), F79 (≠ Y101), G103 (= G125), E106 (= E128), P125 (= P147), E126 (= E148), V131 (≠ A153), P133 (≠ A155), G134 (≠ L156), L219 (≠ A242), F229 (≠ S254)
- binding Butyryl Coenzyme A: F225 (= F250), F241 (= F266)
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
38% identity, 88% coverage: 31:275/277 of query aligns to 20:266/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
34% identity, 93% coverage: 19:275/277 of query aligns to 1:256/258 of 1mj3A
- active site: A68 (≠ S83), M73 (≠ V88), S83 (≠ P97), L85 (≠ H99), G109 (= G125), E112 (= E128), P131 (= P147), E132 (= E148), T137 (≠ A153), P139 (≠ A155), G140 (≠ L156), K225 (vs. gap), F235 (≠ S254)
- binding hexanoyl-coenzyme a: K26 (≠ A41), A27 (≠ V42), L28 (= L43), A30 (= A45), A66 (= A81), G67 (= G82), A68 (≠ S83), D69 (= D84), I70 (≠ L85), G109 (= G125), P131 (= P147), E132 (= E148), L135 (≠ V151), G140 (≠ L156)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
35% identity, 92% coverage: 20:275/277 of query aligns to 1:252/254 of 2dubA
- active site: A67 (≠ S83), M72 (≠ V88), S82 (≠ A98), G105 (= G125), E108 (= E128), P127 (= P147), E128 (= E148), T133 (≠ A153), P135 (≠ A155), G136 (≠ L156), K221 (vs. gap), F231 (≠ S254)
- binding octanoyl-coenzyme a: K25 (≠ A41), A26 (≠ V42), L27 (= L43), A29 (= A45), A65 (= A81), A67 (≠ S83), D68 (= D84), I69 (≠ L85), K70 (= K86), G105 (= G125), E108 (= E128), P127 (= P147), E128 (= E148), G136 (≠ L156), A137 (≠ G157)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
34% identity, 93% coverage: 19:275/277 of query aligns to 1:258/260 of 1dubA
- active site: A68 (≠ S83), M73 (≠ V88), S83 (≠ A102), L87 (≠ I105), G111 (= G125), E114 (= E128), P133 (= P147), E134 (= E148), T139 (≠ A153), P141 (≠ A155), G142 (≠ L156), K227 (vs. gap), F237 (≠ S254)
- binding acetoacetyl-coenzyme a: K26 (≠ A41), A27 (≠ V42), L28 (= L43), A30 (= A45), A66 (= A81), A68 (≠ S83), D69 (= D84), I70 (≠ L85), Y107 (≠ L121), G110 (= G124), G111 (= G125), E114 (= E128), P133 (= P147), E134 (= E148), L137 (≠ V151), G142 (≠ L156), F233 (= F250), F249 (= F266)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
34% identity, 93% coverage: 19:275/277 of query aligns to 31:288/290 of P14604
- E144 (= E128) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E148) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
34% identity, 92% coverage: 21:275/277 of query aligns to 1:256/258 of 1ey3A
- active site: A66 (≠ S83), M71 (≠ V88), S81 (≠ A102), L85 (≠ I105), G109 (= G125), E112 (= E128), P131 (= P147), E132 (= E148), T137 (≠ A153), P139 (≠ A155), G140 (≠ L156), K225 (vs. gap), F235 (≠ S254)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ A41), L26 (= L43), A28 (= A45), A64 (= A81), G65 (= G82), A66 (≠ S83), D67 (= D84), I68 (≠ L85), L85 (≠ I105), W88 (≠ F108), G109 (= G125), P131 (= P147), L135 (≠ V151), G140 (≠ L156)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
31% identity, 94% coverage: 19:277/277 of query aligns to 1:260/260 of 2hw5C
- active site: A68 (≠ S83), M73 (≠ V88), S83 (≠ A98), L87 (≠ A102), G111 (= G125), E114 (= E128), P133 (= P147), E134 (= E148), T139 (≠ A153), P141 (≠ A155), G142 (≠ L156), K227 (≠ Q244), F237 (≠ S254)
- binding crotonyl coenzyme a: K26 (≠ A41), A27 (≠ V42), L28 (= L43), A30 (= A45), K62 (≠ R77), I70 (≠ L85), F109 (≠ L123)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
36% identity, 93% coverage: 21:277/277 of query aligns to 4:261/261 of 5jbxB
- active site: A67 (≠ S83), R72 (vs. gap), L84 (≠ Y101), R88 (vs. gap), G112 (= G125), E115 (= E128), T134 (≠ P147), E135 (= E148), I140 (≠ A153), P142 (≠ A155), G143 (≠ L156), A228 (≠ Q244), L238 (≠ S254)
- binding coenzyme a: S24 (≠ A41), R25 (≠ V42), R26 (≠ L43), A28 (= A45), A65 (= A81), D68 (= D84), L69 (= L85), K70 (= K86), L110 (= L123), G111 (= G124), T134 (≠ P147), E135 (= E148), L138 (≠ V151), R168 (≠ P181)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
34% identity, 91% coverage: 26:277/277 of query aligns to 12:266/266 of O53561
- K135 (≠ R143) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 143:150, 38% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ R150) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
Q8GYN9 1,4-dihydroxy-2-naphthoyl-CoA synthase, peroxisomal; DHNS; Enoyl-CoA hydratase/isomerase D; ECHID; Naphthoate synthase; EC 4.1.3.36 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 87% coverage: 32:272/277 of query aligns to 87:328/337 of Q8GYN9
Sites not aligning to the query:
- 20 H→V: Loss of peroxisomal targeting.
Q7CQ56 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
33% identity, 87% coverage: 32:272/277 of query aligns to 34:276/285 of Q7CQ56
3h02A 2.15 angstrom resolution crystal structure of naphthoate synthase from salmonella typhimurium.
32% identity, 87% coverage: 32:272/277 of query aligns to 30:257/266 of 3h02A