SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_157123969.1 NCBI__GCF_900177405.1:WP_157123969.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
56% identity, 93% coverage: 19:267/267 of query aligns to 10:257/257 of P0AAH0

query
sites
P0AAH0

R

K

I

I

R

Q

A

V

E

R

N

N

V

L

S

N

V

F

F

Y

Y

Y

G

G

N

K

K

F

K

H

A

A

I

L

D

K

D

N

V

I

S

N

I

L

D

D

I

I

D

A

Q

K

D

N

N

Q

V

V

T

T

A

A

F

F

I

I

G

G

P

P

S

S

G

G

C

C

G
|
G

K
|
K

S

S

T

T

F

L

L

L

R

R

T

T

L

F

N

N

R

K

M

M

N

F

D

E

T

L

I

Y

A

P

S

E

A

Q

R

R

V

A

E

E

G

G

R

E

I

I

T

L

L

L

D

D

G

G

E

D

D

N

I

I

Y

L

S

T

S

N

G

S

M

Q

D

D

V

I

V

A

Q

L

L

L

R

R

A

A

R

K

V

V

G

G

M

M

V

V

F

F

Q

Q

K

K

P

P

N

T

P

P

F

F

P

P

K

M

S

S

I

I

F

Y

D

D

N

N

V

I

A

A

Y

F

G

G

P

V

R

R

I

L

H

F

G

E

L

-

A

K

T

L

S

S

K

R

S

A

E

D

L

M

E

D

G

E

I

R

V

V

E

Q

R

W

S

A

L

L

K

T

R

K

A

A

G

A

L

L

W

W

D

N

E

E

V

T

K

K

D

D

R

K

L

L

A

H

D

Q

S

S

G

G

T

Y

A

S

L

L

S

S

G

G

Q

Q

R

R

L

L

C

C

I

I

A

A

R

R

A

G

I

I

A

A

V

I

D

R

P

P

E

E

V

V

I

L

L

L

M

L

D

D

E

E

P

P

C

C

S

S

A

A

L

L

D

D

P

P

I

I

A

S

T

T

A

G

K

R

I

I

E

E

L

L

I

I

H

T

E

E

L

L

R

K

G

Q

R

D

Y

Y

A

T

I

V

V

V

I

I

V

V

T

T

H

H

N

N

M

M

Q

Q

A

A

R

R

V

C

S

S

Q

D

R

H

T

T

A

A

F

F

F

M

H

Y

L

L

G

G

H

E

L

L

V

I

E

E

Y

F

G

S

D

N

T

T

S

D

A

D

I

L

F

F

T

T

N

K

P

P

T

A

E

K

T

K

R

Q

T

T

K

E

D

D

Y

Y

I

I

T

T

G

G

R

R

Y

Y

G

G

G48 (= G57) mutation to I: Loss of phosphate transport.
K49 (= K58) mutation to Q: Loss of phosphate transport.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
36% identity, 92% coverage: 18:262/267 of query aligns to 1:236/241 of 4u00A

query
sites
4u00A

P

P

R

I

I

I

R

R

A

I

E

R

N

N

V

L

S

H

V

K

F

W

Y
x
F

G

G

N

P

K

L

K

H

A
x
V

I

L

D

K

D

G

V

I

S

H

I

L

D

E

I

V

D

A

Q

P

D

G

N

E

V

K

T

L

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

T

T

L

I

N

N

R

R

M

L

N

E

D

D

T

F

I

-

A

-

S

-

A

-

R

-

V

Q

E

E

G

G

R

E

I

V

T

V

L

V

D

D

G

G

E

L

D

S

I

V

Y

K

S

D

G

-

M

R

D

A

V

L

V

R

Q

E

L

I

R

R

A

R

R

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

Q

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

F

L

D

E

N

N

V

V

A

T

Y

L

G

A

P

P

-

M

R

R

I

V

H

R

G

R

L

W

A

P

T

R

S

E

K

K

S

A

E

E

L

K

E

K

G

A

I

L

V

-

E

-

R

E

S

L

L

L

K

E

R

R

A

V

G

G

L

I

W

L

D

D

E

Q

V

A

K

R

D

K

R

Y

L

P

A

A

D

Q

S

-

G

-

T

-

A

-

L

L

S

S

G

G

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

K

E

I

V

E

L

E

D

L

V

I

M

H

R

E

D

L

L

-

A

R

Q

G

G

R

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

A

Q

D

R

R

T

V

A

V

F

F

F

M

H

D

L

G

G

G

H

Q

L

I

V

V

E

E

Y

E

G

G

D

R

T

P

S

E

A

E

I

I

F

F

T

T

N

R

P

P

T

K

E

E

T

E

R

R

T

T

K

R

D

S

Y

F

I

L

binding adenosine-5'-diphosphate: F12 (≠ Y29), V17 (≠ A34), S37 (= S54), G38 (= G55), S39 (≠ C56), G40 (= G57), K41 (= K58), S42 (= S59), T43 (= T60)

3c4jA Abc protein artp in complex with atp-gamma-s
34% identity, 89% coverage: 29:266/267 of query aligns to 13:242/242 of 3c4jA

query
sites
3c4jA

Y
x
F

G

G

N

S

K

L

K

E

A
x
V

I

L

D

K

D

G

V

I

S

N

I

V

D

H

I

I

D

R

Q

E

D

G

N

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

L

N

N

R

L

M

L

N

E

D

D

T

F

I

D

A

-

S

-

A

-

R

-

V

-

E

E

G

G

R

E

I

I

T

I

L

I

D

D

G

G

E

I

D

N

I

L

Y

K

S

A

S

K

G

D

M

T

D

N

V

L

V

N

Q

K

L

V

R

R

A

E

R

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

F

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

R

K

I

V

H

R

G

K

L

W

A

P

T

R

S

E

K

K

S

A

E

E

L

A

E

K

G

A

I

M

V

-

E

-

R

E

S

L

L

L

K

D

R

K

A

V

G

G

L

L

W

-

D

-

E

-

V

-

K

K

D

D

R

K

L

A

A

H

D

A

S

Y

G

P

T

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

K

E

I

V

E

L

E

S

L

V

I

M

H

K

E

Q

L

L

R

A

G

N

R

E

-

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

D

K

T

P

S

E

A

D

I

L

F

F

T

D

N

R

P

P

T

Q

E

H

T

E

R

R

T

T

K

K

D

A

Y

F

I

L

T

S

G

K

R

V

Y

F

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y29), V18 (≠ A34), S38 (= S54), G39 (= G55), S40 (≠ C56), G41 (= G57), K42 (= K58), S43 (= S59), T44 (= T60)

3c41J Abc protein artp in complex with amp-pnp/mg2+
34% identity, 89% coverage: 29:266/267 of query aligns to 13:242/242 of 3c41J

query
sites
3c41J

Y
x
F

G

G

N

S

K

L

K

E

A
x
V

I

L

D

K

D

G

V

I

S

N

I

V

D

H

I

I

D

R

Q

E

D

G

N

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

L

N

N

R

L

M

L

N

E

D

D

T

F

I

D

A

-

S

-

A

-

R

-

V

-

E

E

G

G

R

E

I

I

T

I

L

I

D

D

G

G

E

I

D

N

I

L

Y

K

S

A

S

K

G

D

M

T

D

N

V

L

V

N

Q

K

L

V

R

R

A

E

R

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

F

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

R

K

I

V

H

R

G

K

L

W

A

P

T

R

S

E

K

K

S

A

E

E

L

A

E

K

G

A

I

M

V

-

E

-

R

E

S

L

L

L

K

D

R

K

A

V

G

G

L

L

W

-

D

-

E

-

V

-

K

K

D

D

R

K

L

A

A

H

D

A

S

Y

G

P

T

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

K

V

I

I

M

L

L

M

F

D
|
D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

K

E

I

V

E

L

E

S

L

V

I

M

H

K

E

Q

L

L

R

A

G

N

R

E

-

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

D

K

T

P

S

E

A

D

I

L

F

F

T

D

N

R

P

P

T

Q

E

H

T

E

R

R

T

T

K

K

D

A

Y

F

I

L

T

S

G

K

R

V

Y

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y29), V18 (≠ A34), S38 (= S54), G39 (= G55), G41 (= G57), K42 (= K58), S43 (= S59), T44 (= T60)
binding magnesium ion: S43 (= S59), D163 (= D188)

2olkA Abc protein artp in complex with adp-beta-s
34% identity, 89% coverage: 29:266/267 of query aligns to 13:242/242 of 2olkA

query
sites
2olkA

Y
x
F

G

G

N

S

K

L

K

E

A
x
V

I

L

D

K

D

G

V

I

S

N

I

V

D

H

I

I

D

R

Q

E

D

G

N

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

L

N

N

R

L

M

L

N

E

D

D

T

F

I

D

A

-

S

-

A

-

R

-

V

-

E

E

G

G

R

E

I

I

T

I

L

I

D

D

G

G

E

I

D

N

I

L

Y

K

S

A

S

K

G

D

M

T

D

N

V

L

V

N

Q

K

L

V

R

R

A

E

R

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

F

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

R

K

I

V

H

R

G

K

L

W

A

P

T

R

S

E

K

K

S

A

E

E

L

A

E

K

G

A

I

M

V

-

E

-

R

E

S

L

L

L

K

D

R

K

A

V

G

G

L

L

W

-

D

-

E

-

V

-

K

K

D

D

R

K

L

A

A

H

D

A

S

Y

G

P

T

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

K

E

I

V

E

L

E

S

L

V

I

M

H

K

E

Q

L

L

R

A

G

N

R

E

-

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

D

K

T

P

S

E

A

D

I

L

F

F

T

D

N

R

P

P

T

Q

E

H

T

E

R

R

T

T

K

K

D

A

Y

F

I

L

T

S

G

K

R

V

Y

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y29), V18 (≠ A34), S38 (= S54), G39 (= G55), S40 (≠ C56), G41 (= G57), K42 (= K58), S43 (= S59), T44 (= T60)

2oljA Abc protein artp in complex with adp/mg2+
34% identity, 89% coverage: 29:266/267 of query aligns to 13:242/242 of 2oljA

query
sites
2oljA

Y
x
F

G

G

N

S

K

L

K

E

A
x
V

I

L

D

K

D

G

V

I

S

N

I

V

D

H

I

I

D

R

Q

E

D

G

N

E

V

V

T

V

A

V

F

V

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

L

N

N

R

L

M

L

N

E

D

D

T

F

I

D

A

-

S

-

A

-

R

-

V

-

E

E

G

G

R

E

I

I

T

I

L

I

D

D

G

G

E

I

D

N

I

L

Y

K

S

A

S

K

G

D

M

T

D

N

V

L

V

N

Q

K

L

V

R

R

A

E

R

E

V

V

G

G

M

M

V

V

F

F

Q

Q

K

R

P

F

N

N

P

L

F

F

P

P

K

H

-

M

S

T

I

V

F

L

D

N

N

N

V

I

A

T

Y

L

G

A

P

P

-

M

R

K

I

V

H

R

G

K

L

W

A

P

T

R

S

E

K

K

S

A

E

E

L

A

E

K

G

A

I

M

V

-

E

-

R

E

S

L

L

L

K

D

R

K

A

V

G

G

L

L

W

-

D

-

E

-

V

-

K

K

D

D

R

K

L

A

A

H

D

A

S

Y

G

P

T

D

A

S

L

L

S

S

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

K

V

I

I

M

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

G

K

E

I

V

E

L

E

S

L

V

I

M

H

K

E

Q

L

L

R

A

G

N

R

E

-

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

L

F

F

F

M

H

D

L

G

G

G

H

Y

L

I

V

I

E

E

Y

E

G

G

D

K

T

P

S

E

A

D

I

L

F

F

T

D

N

R

P

P

T

Q

E

H

T

E

R

R

T

T

K

K

D

A

Y

F

I

L

T

S

G

K

R

V

Y

F

binding adenosine-5'-diphosphate: F13 (≠ Y29), V18 (≠ A34), S38 (= S54), G39 (= G55), S40 (≠ C56), G41 (= G57), K42 (= K58), S43 (= S59), T44 (= T60)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
35% identity, 88% coverage: 29:263/267 of query aligns to 11:237/240 of 4ymuJ

query
sites
4ymuJ

Y
x
F

G

G

N

S

K

L

K

E

A
x
V

I

L

D

K

D

G

V

V

S

T

I

L

D

K

I

V

D

N

Q

K

D

G

N

E

V

V

T

V

A

V

F

I

I

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

T

C

L

I

N

N

R

L

M

L

N

E

D

E

T

P

I

-

A

-

S

-

A

-

R

-

V

T

E

K

G

G

R

E

I

V

T

F

L

I

D

D

G

G

E

V

D

K

I

I

Y

N

S

N

S

G

G

K

M

V

D

N

V

I

V

N

Q

K

L

V

R

R

A

Q

R

K

V

V

G

G

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

F

P

P

K

H

-

L

S

T

I

A

F

I

D

E

N

N

V

I

A

T

Y

L

G

A

P

P

R

-

I

V

H

K

G

V

L

K

A

K

T

M

S

N

K

K

S

K

E

E

L

A

E

E

G

E

I

L

V

A

E

V

R

D

S

L

L

L

K

A

R

K

A

V

G

G

L

L

W

L

D

D

E

K

V

-

K

K

D

D

R

Q

L

Y

A

P

D

-

S

-

G

-

T

I

A

K

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

L

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

Q

P

P

E

E

V

V

I

M

L

L

M

F

D

D

E
|
E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

M

T

V

A

K

K

E

I

V

E

L

E

N

L

V

I

M

H

K

E

Q

L

L

R

A

G

N

R

E

-

G

Y

M

A

T

I

M

V

V

I

V

V

V

T

T

H
|
H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

E

V

V

S

G

Q

D

R

R

T

V

A

I

F

F

F

M

H

D

L

D

G

G

H

V

L

I

V

V

E

E

Y

E

G

G

D

T

T

P

S

E

A

E

I

I

F

F

T

Y

N

R

P

A

T

K

E

N

T

E

R

R

T

T

K

R

D

E

Y

F

I

L

T

S

binding adenosine-5'-triphosphate: F11 (≠ Y29), V16 (≠ A34), S36 (= S54), G37 (= G55), S38 (≠ C56), G39 (= G57), K40 (= K58), S41 (= S59), T42 (= T60), E162 (= E189), H194 (= H220)
binding magnesium ion: S41 (= S59), E162 (= E189)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
34% identity, 85% coverage: 37:262/267 of query aligns to 44:263/382 of 7ahhC

query
sites
7ahhC

D

D

V

T

S

N

I

F

D

E

I

I

D

N

Q

E

D

G

N

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S

S

G
|
G

C

S

G
|
G

K
|
K

S

S

T

T

F

L

L

L

R

R

T

L

L

L

N

N

R

R

M

L

N

I

D

E

T

P

I

T

A

S

S

-

A

-

R

-

V

-

E

-

G

G

R

K

I

I

T

F

L

I

D

D

G

N

E

Q

D

D

I

V

Y

A

S

T

-

L

S

N

G

K

M

E

D

D

V

L

Q

Q

L

V

R

R

A

R

R

K

-

T

V

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

F

L

D

E

N

N

V

T

A

E

Y

Y

G

G

P

L

R

E

I

V

H

Q

G

N

L

V

A

-

T

-

S

P

K

K

S

E

E

E

L

R

E

R

G

K

I

R

V

A

E

E

R

K

S

A

L

L

K

D

R

N

A

A

G

N

L

L

W

L

D

D

E

-

V

F

K

K

D

D

R

Q

L

Y

A

P

D

K

S

Q

G

-

T

-

A

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

I

L

A

A

V

N

D

D

P

P

E

E

V

I

I

L

L

L

M

M

D
|
D

E
|
E

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

A

R

K

E

I

M

E

Q

E

D

L

E

I

L

H

L

E

E

L

L

R

Q

G

A

R

K

Y

F

-

Q

-

K

A

T

I

I

V

I

I

F

V

V

T

S

H

H

N

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

Q

D

R

R

T

I

A

A

F

I

F

M

H

K

L

D

G

G

H

K

L

I

V

M

E

Q

Y

I

G

G

D

T

T

G

S

E

A

E

I

I

F

L

T

T

N

N

P

P

T

A

E

N

T

D

R

Y

T

V

K

K

D

T

Y

F

I

V

binding phosphoaminophosphonic acid-adenylate ester: G62 (= G55), G64 (= G57), K65 (= K58), D187 (= D188), E188 (= E189)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
34% identity, 85% coverage: 37:262/267 of query aligns to 44:263/382 of 7aheC

query
sites
7aheC

D

D

V

T

S

N

I

F

D

E

I

I

D

N

Q

E

D

G

N

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S

S

G

G

C

S

G

G

K

K

S

S

T

T

F

L

L

L

R

R

T

L

L

L

N

N

R

R

M

L

N

I

D

E

T

P

I

T

A

S

S

-

A

-

R

-

V

-

E

-

G

G

R

K

I

I

T

F

L

I

D

D

G

N

E

Q

D

D

I

V

Y

A

S

T

-

L

S

N

G

K

M

E

D

D

V

L

Q

Q

L

V

R

R

A

R

R

K

-

T

V

M

G

S

M

M

V

V

F

F

Q

Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

F

L

D

E

N

N

V

T

A

E

Y

Y

G

G

P

L

R

E

I

V

H

Q

G

N

L

V

A

-

T

-

S

P

K

K

S

E

E

E

L

R

E

R

G

K

I

R

V

A

E

E

R

K

S

A

L

L

K

D

R

N

A

A

G

N

L

L

W

L

D

D

E

-

V

F

K

K

D

D

R

Q

L

Y

A

P

D

K

S

Q

G

-

T

-

A

-

L

L

S

S

G

G

Q

M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

I

L

A

A

V

N

D

D

P

P

E

E

V

I

I

L

L

L

M

M

D

D

E

E

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

A

R

K

E

I

M

E

Q

E

D

L

E

I

L

H

L

E

E

L

L

R

Q

G

A

R

K

Y

F

-

Q

-

K

A

T

I

I

V

I

I

F

V

V

T

S

H

H

N

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

Q

D

R

R

T

I

A

A

F

I

F

M

H

K

L

D

G

G

H

K

L

I

V

M

E

Q

Y

I

G

G

D

T

T

G

S

E

A

E

I

I

F

L

T

T

N

N

P

P

T

A

E

N

T

D

R

Y

T

V

K

K

D

T

Y

F

I

V

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298

7ahdC Opua (e190q) occluded (see paper)
34% identity, 85% coverage: 37:262/267 of query aligns to 44:259/260 of 7ahdC

query
sites
7ahdC

D

D

V

T

S

N

I

F

D

E

I

I

D

N

Q

E

D

G

N

E

V

I

T

F

A

V

F

I

I

M

G

G

P

L

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

R

T

L

L

L

N

N

R

R

M

L

N

I

D

E

T

P

I

T

A

S

S

-

A

-

R

-

V

-

E

-

G

G

R

K

I

I

T

F

L

I

D

D

G

N

E

Q

D

D

I

V

Y

A

S

T

-

L

S

N

G

K

M

E

D

D

V

L

Q

Q

L

V

R

R

A

R

R

K

-

T

V

M

G

S

M

M

V

V

F

F

Q
|
Q

K

N

P

F

N

G

P

L

F

F

P

P

-

H

K

R

S

T

I

I

F

L

D

E

N

N

V

T

A

E

Y

Y

G

G

P

L

R

E

I

V

H

Q

G

N

L

V

A

P

T

-

S

-

K

K

S

E

E

E

L

R

E

R

G

K

I

R

V

A

E

E

R

K

S

A

L

L

K

D

R

N

A

A

G

N

L

L

W

L

D

D

E

-

V

F

K

K

D

D

R

Q

L

Y

A

P

D
x
K

S
x
Q

G

-

T

-

A

-

L

L

S
|
S

G

G

G
|
G

Q
x
M

Q

Q

R

R

L

V

C

G

I

L

A

A

R

R

A

A

I

L

A

A

V

N

D

D

P

P

E

E

V

I

I

L

L

L

M

M

D

D

E
x
Q

P

A

C

F

S

S

A

A

L

L

D

D

P

P

I

L

A

I

T

R

A

R

K

E

I

M

E

Q

E

D

L

E

I

L

H

L

E

E

L

L

R

Q

G

A

R

K

Y

F

-

Q

-

K

A

T

I

I

V

I

I

F

V

V

T

S

H
|
H

N

D

M

L

Q

N

Q

E

A

A

A

L

R

R

V

I

S

G

Q

D

R

R

T

I

A

A

F

I

F

M

H

K

L

D

G

G

H

K

L

I

V

M

E

Q

Y

I

G

G

D

T

T

G

S

E

A

E

I

I

F

L

T

T

N

N

P

P

T

-

E

-

T

-

R

-

T

A

K

N

D

D

Y

Y

I

V

binding adenosine-5'-triphosphate: S61 (= S54), G62 (= G55), G64 (= G57), K65 (= K58), S66 (= S59), T67 (= T60), Q111 (= Q107), K161 (≠ D159), Q162 (≠ S160), S164 (= S165), G166 (= G167), M167 (≠ Q168), Q188 (≠ E189), H221 (= H220)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 39

5x40A Structure of a cbio dimer bound with amppcp (see paper)
34% identity, 88% coverage: 18:252/267 of query aligns to 3:230/280 of 5x40A

query
sites
5x40A

P

P

R

I

I

L

R

A

A

A

E

E

N

A

V

L

S

T

-

Y

V

A

F
|
F

Y

P

G

G

N

G

K
x
V

K

K

A
|
A

I

L

D

D

V

L

S

S

I

L

D

A

I

V

D

P

Q

K

D

G

N

E

V

S

T

L

A

A

F

I

I

L

G

G

P

P

S
x
N

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

L

R

L

T

H

L

L

N

N

R

-

M

-

N

-

D

-

T

-

I

-

A

G

S

T

A

L

R

R

V

P

E

Q

-

S

G

G

R

R

I

V

T

L

L

L

D

G

G

G

E

T

D

A

I

T

Y

G

S

H

S

S

G

R

M

K

D

D

V

L

V

T

Q

G

L

W

R

R

A

R

R

R

V

V

G

G

M

L

V

V

F

L

Q
|
Q

K

D

P

A

N

D

P

D

-

Q

-

L

F

F

P

A

K

T

S

T

I

V

F

F

D

E

N

D

V

V

A

S

Y

F

G

G

P

P

R

L

I

N

H

L

G

G

L

L

A

-

T

-

S

S

K

E

S

A

E

E

L

A

E

R

G

A

I

R

V

V

E

E

R

E

S

A

L

L

K

A

R

A

A

L

G

S

L

I

W

S

D

D

E

-

V

L

K

R

D

D

R

R

L

-

A

-

D

-

S

P

G

T

T
x
H

A
x
M

L
|
L

S
|
S

G

G

G
|
G

Q
|
Q

Q

K

Q

R

R

R

L

V

C

A

I

I

A

A

R

G

A

A

I

V

A

A

V

M

D

R

P

P

E

E

V

V

I

L

L

L

M

L

D

D

E
x
Q

P

P

C

T

S

A

A

G

L

L

D

D

P

L

I

A

A

G

T

T

A

E

K

Q

I

L

E

L

E

T

L

L

I

L

H

R

E

G

L

L

R

R

G

A

R

A

-

G

Y

M

A

T

I

L

V

V

I

F

V

S

T

T

H
|
H

N

D

M

V

Q

E

Q

L

A

A

R

A

V

L

S

A

Q

D

R

R

T

V

A

A

F

L

F

F

H

R

L

T

G

G

H

R

L

V

V

L

E

A

Y

E

G

G

D

A

T

A

S

E

A

A

I

V

F

L

T

S

N

D

binding phosphomethylphosphonic acid adenylate ester: F14 (= F28), V18 (≠ K32), A20 (= A34), N40 (≠ S54), G41 (= G55), G43 (= G57), K44 (= K58), S45 (= S59), T46 (= T60), Q88 (= Q107), H139 (≠ T162), M140 (≠ A163), L141 (= L164), S142 (= S165), G144 (= G167), Q145 (= Q168), Q166 (≠ E189), H198 (= H220)
binding magnesium ion: S45 (= S59), Q88 (= Q107)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 91% coverage: 20:262/267 of query aligns to 2:241/343 of P30750

query
sites
P30750

I

I

R

K

A

L

E

S

N

N

V

I

S

T

-

K

V

V

F

F

Y

H

G

Q

N

G

K

T

K

R

-

T

-

I

-

Q

A

A

I

L

D

N

V

V

S

S

I

L

D

H

I

V

D

P

Q

A

D

G

N

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

T

C

L

V

N

N

R

L

M

L

N

E

D

R

T

P

I

-

A

-

S

-

A

-

R

-

V

T

E

E

G

G

R

S

I

V

T

L

L

V

D

D

G

G

E

Q

D

E

I

L

Y

T

S

T

-

L

S

S

G

E

M

S

D

E

V

L

V

T

Q

K

L

A

R

R

A

R

R

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

D

G

N

N

V

V

A

A

Y

L

G

P

P

L

R

E

I

L

H

D

G

N

L

-

A

-

T

T

S

P

K

K

S

D

E

E

L

V

E

K

G

R

I

R

V

V

E

T

R

E

S

L

L

L

K

S

R

L

A

V

G

G

L

L

W

G

D

D

E

K

V

-

K

-

D

-

R

-

L

-

A

H

D

D

S

S

-

Y

G

P

T

S

A

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

D

N

P

P

E

K

V

V

I

L

L

L

M

C

D

D

E
|
E

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

K

S

I

I

E

L

E

E

L

L

I

L

H

K

E

D

L

I

R

N

G

R

R

R

-

L

-

G

Y

L

A

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

Q

D

R

C

T

V

A

A

F

V

F

I

H

S

L

N

G

G

H

E

L

L

V

I

E

E

Y

Q

G

D

D

T

T

V

S

S

A

E

I

V

F

F

T

S

N

H

P

P

T

K

E

T

T

P

R

L

T

A

K

Q

D

K

Y

F

I

I

40:46 (vs. 54:60, 86% identical) binding ATP
E166 (= E189) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
32% identity, 91% coverage: 20:262/267 of query aligns to 3:242/344 of 6cvlD

query
sites
6cvlD

I

I

R

K

A

L

E

S

N

N

V

I

S

T

-

K

V

V

F
|
F

Y

H

G
x
Q

N

G

K

T

K

R

-

T

-
x
I

-

Q

A

A

I

L

D

N

V

V

S

S

I

L

D

H

I

V

D

P

Q

A

D

G

N

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

T

C

L

V

N

N

R

L

M

L

N

E

D

R

T

P

I

-

A

-

S

-

A

-

R

-

V

T

E

E

G

G

R

S

I

V

T

L

L

V

D

D

G

G

E

Q

D

E

I

L

Y

T

S

T

-

L

S

S

G

E

M

S

D

E

V

L

V

T

Q

K

L

A

R

R

A

R

R

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

D

G

N

N

V

V

A

A

Y

L

G

P

P

L

R

E

I

L

H

D

G

N

L

-

A

-

T

T

S

P

K

K

S

D

E

E

L

V

E

K

G

R

I

R

V

V

E

T

R

E

S

L

L

L

K

S

R

L

A

V

G

G

L

L

W

G

D

D

E

K

V

-

K

-

D

-

R

-

L

-

A

H

D

D

S

S

-

Y

G

P

T

S

A
x
N

L

L

S
|
S

G

G

Q
|
Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

D

N

P

P

E

K

V

V

I

L

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

K

S

I

I

E

L

E

E

L

L

I

L

H

K

E

D

L

I

R

N

G

R

R

R

-

L

-

G

Y

L

A

T

I

I

V

L

I

L

V

I

T

T

H
|
H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

Q

D

R

C

T

V

A

A

F

V

F

I

H

S

L

N

G

G

H

E

L

L

V

I

E

E

Y

Q

G

D

D

T

T

V

S

S

A

E

I

V

F

F

T

S

N

H

P

P

T

K

E

T

T

P

R

L

T

A

K

Q

D

K

Y

F

I

I

binding phosphothiophosphoric acid-adenylate ester: F12 (= F28), Q14 (≠ G30), I19 (vs. gap), S41 (= S54), G42 (= G55), A43 (≠ C56), G44 (= G57), K45 (= K58), S46 (= S59), T47 (= T60), N141 (≠ A163), S143 (= S165), Q146 (= Q168), H200 (= H220)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
32% identity, 91% coverage: 20:262/267 of query aligns to 3:242/344 of 3tuzC

query
sites
3tuzC

I

I

R

K

A

L

E

S

N

N

V

I

S

T

-

K

V

V

F
|
F

Y

H

G
x
Q

N

G

K

T

K

R

-

T

-

I

-

Q

A

A

I

L

D

N

V

V

S

S

I

L

D

H

I

V

D

P

Q

A

D

G

N

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S

S

G
|
G

C

A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

T

C

L

V

N

N

R

L

M

L

N

E

D

R

T

P

I

-

A

-

S

-

A

-

R

-

V

T

E

E

G

G

R

S

I

V

T

L

L

V

D

D

G

G

E

Q

D

E

I

L

Y

T

S

T

-

L

S

S

G

E

M

S

D

E

V

L

V

T

Q

K

L

A

R

R

A

R

R

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

D

G

N

N

V

V

A

A

Y

L

G

P

P

L

R

E

I

L

H

D

G

N

L

-

A

-

T

T

S

P

K

K

S

D

E

E

L

V

E

K

G

R

I

R

V

V

E

T

R

E

S

L

L

L

K

S

R

L

A

V

G

G

L

L

W

G

D

D

E

K

V

-

K

-

D

-

R

-

L

-

A

H

D

D

S

S

-

Y

G

P

T

S

A

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

D

N

P

P

E

K

V

V

I

L

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

K

S

I

I

E

L

E

E

L

L

I

L

H

K

E

D

L

I

R

N

G

R

R

R

-

L

-

G

Y

L

A

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

Q

D

R

C

T

V

A

A

F

V

F

I

H

S

L

N

G

G

H

E

L

L

V

I

E

E

Y

Q

G

D

D

T

T

V

S

S

A

E

I

V

F

F

T

S

N

H

P

P

T

K

E

T

T

P

R

L

T

A

K

Q

D

K

Y

F

I

I

binding adenosine-5'-diphosphate: F12 (= F28), Q14 (≠ G30), G42 (= G55), G44 (= G57), K45 (= K58), S46 (= S59), T47 (= T60)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
32% identity, 91% coverage: 20:262/267 of query aligns to 3:242/344 of 3tuiC

query
sites
3tuiC

I

I

R

K

A

L

E

S

N

N

V

I

S

T

-

K

V

V

F
|
F

Y

H

G

Q

N

G

K

T

K

R

-

T

-

I

-

Q

A

A

I

L

D

N

V

V

S

S

I

L

D

H

I

V

D

P

Q

A

D

G

N

Q

V

I

T

Y

A

G

F

V

I

I

G

G

P

A

S

S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

F

L

L

I

R

R

T

C

L

V

N

N

R

L

M

L

N

E

D

R

T

P

I

-

A

-

S

-

A

-

R

-

V

T

E

E

G

G

R

S

I

V

T

L

L

V

D

D

G

G

E

Q

D

E

I

L

Y

T

S

T

-

L

S

S

G

E

M

S

D

E

V

L

V

T

Q

K

L

A

R

R

A

R

R

Q

V

I

G

G

M

M

V

I

F

F

Q

Q

K

H

P

F

N

N

P

L

F

L

P

S

-

S

K

R

S

T

I

V

F

F

D

G

N

N

V

V

A

A

Y

L

G

P

P

L

R

E

I

L

H

D

G

N

L

-

A

-

T

T

S

P

K

K

S

D

E

E

L

V

E

K

G

R

I

R

V

V

E

T

R

E

S

L

L

L

K

S

R

L

A

V

G

G

L

L

W

G

D

D

E

K

V

-

K

-

D

-

R

-

L

-

A

H

D

D

S

S

-

Y

G

P

T

S

A

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

C

A

I

I

A

A

R

R

A

A

I

L

A

A

V

S

D

N

P

P

E

K

V

V

I

L

L

L

M

C

D

D

E

Q

P

A

C

T

S

S

A

A

L

L

D

D

P

P

I

A

A

T

T

T

A

R

K

S

I

I

E

L

E

E

L

L

I

L

H

K

E

D

L

I

R

N

G

R

R

R

-

L

-

G

Y

L

A

T

I

I

V

L

I

L

V

I

T

T

H

H

N

E

M

M

Q

D

Q

V

A

V

A

K

R

R

V

I

S

C

Q

D

R

C

T

V

A

A

F

V

F

I

H

S

L

N

G

G

H

E

L

L

V

I

E

E

Y

Q

G

D

D

T

T

V

S

S

A

E

I

V

F

F

T

S

N

H

P

P

T

K

E

T

T

P

R

L

T

A

K

Q

D

K

Y

F

I

I

binding adenosine-5'-diphosphate: F12 (= F28), G42 (= G55), A43 (≠ C56), G44 (= G57), K45 (= K58), S46 (= S59), T47 (= T60)

1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
34% identity, 88% coverage: 29:264/267 of query aligns to 12:251/258 of 1b0uA

query
sites
1b0uA

Y
|
Y

G

G

N

G

K
x
H

K

E

A

V

I

L

D

K

D

G

V

V

S

S

I

L

D

Q

I

A

D

R

Q

A

D

G

N

D

V

V

T

I

A

S

F

I

I

I

G

G

P

S

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

I

N

N

R

F

M

L

N

E

D

K

T

P

I

-

A

-

S

-

A

-

R

-

V

S

E

E

G

G

R

A

I

I

T

I

L

V

D

N

G

G

E

Q

D

N

I

I

-

N

-

L

-

V

-

R

-

D

-

K

-

D

-

G

-

Q

-

L

-

K

Y

V

S

A

S

D

G

K

M

N

D

Q

V

L

V

R

Q

L

L

L

R

R

A

T

R

R

V

L

G

T

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

W

P

S

K

H

-

M

S

T

I

V

F

L

D

E

N

N

V

V

A

M

Y

E

G

A

P

P

R

-

I

I

H

Q

G

V

L

L

A

G

T

L

S

S

K

K

S

H

E

D

L

A

E

R

G

E

I

R

V

A

E

L

R

K

S

Y

L

L

K

A

R

K

A

V

G

G

L

I

W

D

D

E

E

R

V

A

K

Q

D

G

R

K

L

Y

A

P

D

-

S

-

G

-

T

V

A

H

L

L

S

S

G

G

Q

Q

R

R

L

V

C

S

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

D

V

V

I

L

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

L

T

V

A

G

K

E

I

V

E

L

E

R

L

I

I

M

H

Q

E

Q

L

L

-

A

-

E

R

E

G

G

R

K

Y

-

A

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

H

V

V

S

S

Q

S

R

H

T

V

A

I

F

F

F

L

H

H

L

Q

G

G

H

K

L

I

V

E

E

E

Y

E

G

G

D

D

T

P

S

E

A

Q

I

V

F

F

T

G

N

N

P

P

T

Q

E

S

T

P

R

R

T

L

K

Q

D

Q

Y

F

I

L

T

K

G

G

binding adenosine-5'-triphosphate: Y12 (= Y29), H15 (≠ K32), S37 (= S54), G38 (= G55), G40 (= G57), K41 (= K58), S42 (= S59), T43 (= T60)

P02915 Histidine/lysine/arginine/ornithine transport ATP-binding protein HisP; EC 7.4.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 88% coverage: 29:264/267 of query aligns to 16:255/258 of P02915

query
sites
P02915

Y

Y

G

G

N

G

K

H

K

E

A

V

I

L

D

K

D

G

V

V

S

S

I

L

D

Q

I

A

D

R

Q

A

D

G

N

D

V

V

T

I

A

S

F

I

I

I

G

G

P

S

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

F

L

L

R

R

T

C

L

I

N

N

R

F

M

L

N

E

D

K

T

P

I

-

A

-

S

-

A

-

R

-

V

S

E

E

G

G

R

A

I

I

T

I

L

V

D

N

G

G

E

Q

D

N

I

I

-

N

-

L

-

V

-

R

-

D

-

K

-

D

-

G

-

Q

-

L

-

K

Y

V

S

A

S

D

G

K

M

N

D

Q

V

L

V

R

Q

L

L

L

R

R

A

T

R

R

V

L

G

T

M

M

V

V

F

F

Q

Q

K

H

P

F

N

N

P

L

F

W

P

S

K

H

-

M

S

T

I

V

F

L

D

E

N

N

V

V

A

M

Y

E

G

A

P

P

R

-

I

I

H

Q

G

V

L

L

A

G

T

L

S

S

K

K

S

H

E

D

L

A

E

R

G

E

I

R

V

A

E

L

R

K

S

Y

L

L

K

A

R

K

A

V

G

G

L

I

W

D

D

E

E

R

V

A

K

Q

D

G

R

K

L

Y

A

P

D

-

S

-

G

-

T

V

A

H

L

L

S

S

G

G

Q

Q

R

R

L

V

C

S

I

I

A

A

R

R

A

A

I

L

A

A

V

M

D

E

P

P

E

D

V

V

I

L

L

L

M

F

D

D

E

E

P

P

C

T

S

S

A

A

L

L

D

D

P

P

I

E

A

L

T

V

A

G

K

E

I

V

E

L

E

R

L

I

I

M

H

Q

E

Q

L

L

-

A

-

E

R

E

G

G

R

K

Y

-

A

T

I

M

V

V

I

V

V

V

T

T

H

H

N

E

M

M

Q

G

Q

F

A

A

A

R

R

H

V

V

S

S

Q

S

R

H

T

V

A

I

F

F

F

L

H

H

L

Q

G

G

H

K

L

I

V

E

E

E

Y

E

G

G

D

D

T

P

S

E

A

Q

I

V

F

F

T

G

N

N

P

P

T

Q

E

S

T

P

R

R

T

L

K

Q

D

Q

Y

F

I

L

T

K

G

G

S41 (= S54) binding ATP
G42 (= G55) binding ATP
G44 (= G57) binding ATP
K45 (= K58) binding ATP
S46 (= S59) binding ATP
T47 (= T60) binding ATP

Q5M244 Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; EC 3.6.3.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
32% identity, 87% coverage: 20:250/267 of query aligns to 3:233/280 of Q5M244

query
sites
Q5M244

I

I

R

S

A

L

E

E

N

N

V

V

S

S

V

Y

F

T

Y

Y

G

Q

N

S

-

G

-

T

-

P

-

F

-

E

K

R

A

A

I

L

D

F

D

D

V

M

S

T

I

V

D

T

I

I

D

K

Q

D

D

G

N

S

V

Y

T

T

A

A

F

F

I

I

G

G

P

H

S

T

G

G

C

S

G

G

K

K

S

S

T

T

F

I

L

M

R

Q

T

L

L

L

N

N

R

G

M

L

N

-

D

-

T

-

I

-

A

-

S

Y

A

L

R

P

V

T

E

S

G

G

R

Q

I

V

T

K

L

V

D

D

G

D

E

T

D

I

I

I

-

N

-

S

Y

Q

S

S

S

K

G

N

M

K

D

E

V

I

V

K

Q

P

L

I

R

R

A

K

R

K

V

V

G

G

M

L

V

V

F

F

Q

Q

K

F

P

P

N

E

P

S

-
x
Q

-

L

F

F

P

A

K

E

S

T

I

V

F

L

D

E

N

D

V

I

A

A

Y

F

G

G

P

P

R

Q

I

N

H

F

G

G

L

V

A

-

T

-

S

S

K

K

S

E

E

E

L

A

E

E

G

Q

I

R

V

A

E

L

R

E

S

S

L

L

K

R

R

L

A

V

G

G

L

L

W

S

D

D

E

E

V

L

K

R

D

D

R

Q

L

-

A

-

D

-

S

N

G

P

T

F

A

D

L

L

S

S

G

G

Q

Q

Q

M

Q

R

R

R

L

V

C

A

I

I

A

A

R

G

A

I

I

L

A

A

V

M

D

Q

P

P

E

D

V

I

I

L

L

V

M

L

D

D

E
|
E

P

P

C

T

S

A

A

G

L

L

D

D

P

P

I

Q

A

G

T

R

A

K

K

E

I

L

E

M

E

S

L

L

I

F

H

K

E

Q

L

L

R

H

-

L

G

S

R

G

Y

I

A

T

I

I

V

V

I

L

V

V

T

T

H

H

N

L

M

M

Q

D

A

V

A

A

R

D

V

Y

S

A

Q

T

R

A

T

V

A

N

F

V

F

M

H

E

L

K

G

G

H

R

L

L

V

V

E

L

Y

S

G

G

D

T

T

P

S

K

A

D

I

V

F

F

Q97 (vs. gap) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-90 in EcfA1.
E171 (= E189) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-163 in EcfA1.

4ayxA Structure of the human mitochondrial abc transporter, abcb10 (rod form b) (see paper)
32% identity, 82% coverage: 35:253/267 of query aligns to 357:569/571 of 4ayxA

query
sites
4ayxA

I

F

D

Q

D

D

V

F

S

S

I

L

D

S

I

I

D

P

Q

S

D

G

N

S

V

V

T

T

A

A

F

L

I

V

G

G

P

P

S
|
S

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

F

V

L

L

R

S

T

L

L

L

N

L

R

R

M

L

N

Y

D

D

T

P

I

-

A

-

S

-

A

-

R

-

V

A

E

S

G

G

R

T

I

I

T

S

L

L

D

D

G

G

E

H

D

D

I

I

Y

R

S

Q

S

-

G

-

M

L

D

N

V

P

V

V

Q

W

L

L

R

R

A

S

R

K

V

I

G

G

M

T

V

V

F

S

Q
|
Q

K

E

P

P

N

I

P

L

F

F

P

S

K

C

S

S

I

I

F

A

D

E

N

N

V

I

A

A

Y

Y

G

G

P

A

R

D

I

D

H

P

G

S

L

S

A

V

T

T

S

A

K

E

S

-

E

E

L

I

E

Q

G

R

I

V

V

A

E

E

R

V

S

A

L

N

K

A

R

V

A

A

G

F

L

I

W

R

D

N

-

F

-

P

-

Q

E

G

V

F

K

N

D

T

R

V

L

V

A

G

D

E

S

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