SitesBLAST
Comparing WP_157362518.1 NCBI__GCF_000504245.1:WP_157362518.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8gy3B Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
53% identity, 65% coverage: 2:128/194 of query aligns to 21:148/154 of 8gy3B
- binding fe2/s2 (inorganic) cluster: G38 (= G19), C39 (= C20), G40 (= G21), G42 (= G23), C44 (= C25), G45 (≠ R26), C47 (= C28), C59 (= C40), C97 (= C79), C100 (= C82), Q101 (= Q83), C132 (= C112), C134 (= C114)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q96 (= Q78), C134 (= C114)
5y6qA Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
45% identity, 66% coverage: 1:129/194 of query aligns to 22:153/157 of 5y6qA
- binding fe2/s2 (inorganic) cluster: G40 (= G19), C41 (= C20), D42 (≠ G21), G44 (= G23), C46 (= C25), G47 (≠ R26), C49 (= C28), C61 (= C40), C101 (= C79), G102 (= G80), C104 (= C82), C136 (= C112), C138 (= C114)
- binding pterin cytosine dinucleotide: Q100 (= Q78), C138 (= C114)
4zohC Crystal structure of glyceraldehyde oxidoreductase (see paper)
45% identity, 65% coverage: 3:128/194 of query aligns to 31:157/161 of 4zohC
- binding fe2/s2 (inorganic) cluster: C47 (= C20), S50 (≠ G23), C52 (= C25), G53 (≠ R26), C55 (= C28), C67 (= C40), C106 (= C79), G107 (= G80), C109 (= C82), C141 (= C112), C143 (= C114)
- binding pterin cytosine dinucleotide: Q105 (= Q78), C143 (= C114)
4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
39% identity, 79% coverage: 6:158/194 of query aligns to 26:174/907 of 4usaA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y18), C40 (= C20), E41 (≠ G21), G43 (= G23), C45 (= C25), G46 (≠ R26), C48 (= C28), R58 (≠ Q38), C60 (= C40), C100 (= C79), G101 (= G80), C103 (= C82), C137 (= C112), C139 (= C114)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q78), C139 (= C114)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 531, 532, 535, 539
- binding hydrocinnamic acid: 255, 425, 494, 497, 535, 626
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
39% identity, 79% coverage: 6:158/194 of query aligns to 26:174/907 of 4us9A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y18), C40 (= C20), E41 (≠ G21), G43 (= G23), C45 (= C25), G46 (≠ R26), C48 (= C28), R58 (≠ Q38), C60 (= C40), C100 (= C79), G101 (= G80), C103 (= C82), C137 (= C112), C139 (= C114)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q78), C139 (= C114)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 3-phenylpropanal: 255, 257, 258, 752
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
39% identity, 79% coverage: 6:158/194 of query aligns to 26:174/907 of 4us8A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y18), C40 (= C20), E41 (≠ G21), G43 (= G23), C45 (= C25), G46 (≠ R26), C48 (= C28), R58 (≠ Q38), C60 (= C40), C100 (= C79), G101 (= G80), C103 (= C82), C137 (= C112), C139 (= C114)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q78), C139 (= C114)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539
- binding benzaldehyde: 255, 255, 394, 425, 425, 425, 425, 497, 497, 501, 531, 535, 535, 626, 626, 626, 694, 696, 697
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4c7yA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with sodium dithionite and sodium sulfide (see paper)
39% identity, 79% coverage: 6:158/194 of query aligns to 26:174/907 of 4c7yA
- binding fe2/s2 (inorganic) cluster: C40 (= C20), E41 (≠ G21), G43 (= G23), C45 (= C25), G46 (≠ R26), C48 (= C28), R58 (≠ Q38), C60 (= C40), C100 (= C79), G101 (= G80), C103 (= C82), C137 (= C112), C139 (= C114)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q78), C139 (= C114)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 535, 539
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
- binding hydrogen peroxide: 696, 697, 869
3fc4A Ethylene glycol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
39% identity, 79% coverage: 6:158/194 of query aligns to 26:174/907 of 3fc4A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y18), C40 (= C20), E41 (≠ G21), G43 (= G23), C45 (= C25), G46 (≠ R26), C48 (= C28), R58 (≠ Q38), C60 (= C40), C100 (= C79), G101 (= G80), C103 (= C82), C137 (= C112), C139 (= C114)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q78), C139 (= C114)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 1,2-ethanediol: 535, 622, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
3fahA Glycerol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
39% identity, 79% coverage: 6:158/194 of query aligns to 26:174/907 of 3fahA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y18), C40 (= C20), E41 (≠ G21), G43 (= G23), C45 (= C25), G46 (≠ R26), C48 (= C28), R58 (≠ Q38), C60 (= C40), C100 (= C79), G101 (= G80), C103 (= C82), C137 (= C112), C139 (= C114)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q78), C139 (= C114)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding glycerol: 416, 535, 622, 683, 696, 697, 869, 884, 889, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
1sijA Crystal structure of the aldehyde dehydrogenase (a.K.A. Aor or mop) of desulfovibrio gigas covalently bound to [aso3]- (see paper)
39% identity, 79% coverage: 6:158/194 of query aligns to 26:174/907 of 1sijA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y18), C40 (= C20), E41 (≠ G21), G43 (= G23), C45 (= C25), G46 (≠ R26), C48 (= C28), R58 (≠ Q38), C60 (= C40), Q99 (= Q78), C100 (= C79), G101 (= G80), C103 (= C82), C137 (= C112), C139 (= C114)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q78), C139 (= C114)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding arsenite: 535, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 698, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
Q46509 Aldehyde oxidoreductase; Molybdenum iron sulfur protein; EC 1.2.99.7 from Megalodesulfovibrio gigas (Desulfovibrio gigas) (see paper)
39% identity, 79% coverage: 6:158/194 of query aligns to 26:174/907 of Q46509
- C40 (= C20) binding [2Fe-2S] cluster
- C45 (= C25) binding [2Fe-2S] cluster
- C48 (= C28) binding [2Fe-2S] cluster
- C60 (= C40) binding [2Fe-2S] cluster
- C100 (= C79) binding [2Fe-2S] cluster
- C103 (= C82) binding [2Fe-2S] cluster
- C137 (= C112) binding [2Fe-2S] cluster
- C139 (= C114) binding [2Fe-2S] cluster
1dgjA Crystal structure of the aldehyde oxidoreductase from desulfovibrio desulfuricans atcc 27774 (see paper)
41% identity, 66% coverage: 3:130/194 of query aligns to 23:155/906 of 1dgjA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y18), G39 (= G19), C40 (= C20), G41 (= G21), G43 (= G23), Q44 (≠ L24), C45 (= C25), G46 (≠ R26), C48 (= C28), R58 (≠ Q38), C60 (= C40), C100 (= C79), G101 (= G80), C103 (= C82), C137 (= C112), C139 (= C114)
- binding pterin cytosine dinucleotide: Q99 (= Q78), C139 (= C114)
Sites not aligning to the query:
- active site: 391, 427, 503, 507, 535, 869, 870
- binding molybdenum (iv)oxide: 424, 535, 698, 869
- binding pterin cytosine dinucleotide: 423, 424, 535, 652, 655, 656, 657, 658, 697, 698, 700, 702, 703, 799, 800, 803, 804, 807, 865, 866, 867, 868, 869
1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
39% identity, 65% coverage: 3:128/194 of query aligns to 24:151/156 of 1ffuD
- binding fe2/s2 (inorganic) cluster: C41 (= C20), S44 (≠ G23), H45 (≠ L24), C46 (= C25), G47 (≠ R26), C49 (= C28), C61 (= C40), C100 (= C79), G101 (= G80), C103 (= C82), C135 (= C112), C137 (= C114)
1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
39% identity, 65% coverage: 3:128/194 of query aligns to 23:150/155 of 1ffvA
- binding fe2/s2 (inorganic) cluster: I38 (≠ Y18), C40 (= C20), S43 (≠ G23), C45 (= C25), G46 (≠ R26), C48 (= C28), C60 (= C40), C99 (= C79), G100 (= G80), C102 (= C82), C134 (= C112), C136 (= C114)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q78), C136 (= C114)
P77165 Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA; EC 1.2.99.6 from Escherichia coli (strain K12) (see paper)
38% identity, 65% coverage: 3:129/194 of query aligns to 82:225/229 of P77165
- C99 (= C20) binding [2Fe-2S] cluster
- C104 (= C25) binding [2Fe-2S] cluster
- G105 (≠ R26) binding [2Fe-2S] cluster
- C107 (= C28) binding [2Fe-2S] cluster
- C119 (= C40) binding [2Fe-2S] cluster
- C158 (= C79) binding [2Fe-2S] cluster
- C161 (= C82) binding [2Fe-2S] cluster
- C208 (= C112) binding [2Fe-2S] cluster
- C210 (= C114) binding [2Fe-2S] cluster
5g5gA Escherichia coli periplasmic aldehyde oxidase (see paper)
38% identity, 65% coverage: 3:129/194 of query aligns to 31:174/175 of 5g5gA
- binding fe2/s2 (inorganic) cluster: G47 (= G19), C48 (= C20), D49 (≠ G21), G51 (= G23), C53 (= C25), G54 (≠ R26), C56 (= C28), C68 (= C40), C107 (= C79), G108 (= G80), C110 (= C82), C157 (= C112), C159 (= C114)
3hrdD Crystal structure of nicotinate dehydrogenase (see paper)
38% identity, 63% coverage: 3:125/194 of query aligns to 25:149/160 of 3hrdD
- binding flavin-adenine dinucleotide: E44 (≠ V22), G45 (= G23), E46 (≠ L24)
- binding fe2/s2 (inorganic) cluster: E40 (≠ Y18), C42 (= C20), S43 (≠ G21), G45 (= G23), C47 (= C25), G48 (≠ R26), C50 (= C28), C62 (= C40), Q100 (= Q78), C101 (= C79), G102 (= G80), C104 (= C82), C136 (= C112), C138 (= C114)
- binding pterin cytosine dinucleotide: Q100 (= Q78), C138 (= C114)
Q0QLF3 Nicotinate dehydrogenase small FeS subunit; NDH; Nicotinic acid hydroxylase small FeS subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
38% identity, 63% coverage: 3:125/194 of query aligns to 25:149/157 of Q0QLF3
- C42 (= C20) binding [2Fe-2S] cluster
- C47 (= C25) binding [2Fe-2S] cluster
- C50 (= C28) binding [2Fe-2S] cluster
- C62 (= C40) binding [2Fe-2S] cluster
- C101 (= C79) binding [2Fe-2S] cluster
- C104 (= C82) binding [2Fe-2S] cluster
- C136 (= C112) binding [2Fe-2S] cluster
- C138 (= C114) binding [2Fe-2S] cluster
2e3tA Crystal structure of rat xanthine oxidoreductase mutant (w335a and f336l) (see paper)
34% identity, 74% coverage: 3:145/194 of query aligns to 24:175/1291 of 2e3tA
- binding flavin-adenine dinucleotide: G44 (= G23)
- binding fe2/s2 (inorganic) cluster: G40 (= G19), C41 (= C20), G42 (= G21), G44 (= G23), C46 (= C25), G47 (≠ R26), C49 (= C28), N69 (≠ Q38), C71 (= C40), Q109 (= Q78), C110 (= C79), C113 (= C82), C145 (= C112), C147 (= C114)
Sites not aligning to the query:
- active site: 740, 775, 853, 857, 885, 1233, 1234
- binding bicarbonate ion: 812, 813, 850, 882, 883, 887, 888, 891
- binding calcium ion: 713, 714, 716, 809, 810, 840, 843, 844, 847, 880, 881
- binding flavin-adenine dinucleotide: 228, 229, 230, 231, 232, 233, 234, 235, 236, 309, 318, 319, 322, 323, 325, 326, 331, 332, 375, 376
- binding fe2/s2 (inorganic) cluster: 717
- binding uric acid: 775, 853, 887, 982, 983, 1051, 1052, 1234
P22985 Xanthine dehydrogenase/oxidase; EC 1.17.1.4; EC 1.17.3.2 from Rattus norvegicus (Rat) (see 2 papers)
38% identity, 59% coverage: 3:117/194 of query aligns to 26:152/1331 of P22985
- C43 (= C20) binding [2Fe-2S] cluster
- C48 (= C25) binding [2Fe-2S] cluster
- C51 (= C28) binding [2Fe-2S] cluster
- C73 (= C40) binding [2Fe-2S] cluster
- C112 (= C79) binding [2Fe-2S] cluster
- C115 (= C82) binding [2Fe-2S] cluster
- C147 (= C112) binding [2Fe-2S] cluster
- C149 (= C114) binding [2Fe-2S] cluster
Sites not aligning to the query:
- 256:263 binding FAD
- 335:336 WF→AL: Converts the enzyme to the oxidase form that utilizes molecular oxygen as electron acceptor. Interferes with normal conversion to the dehydrogenase form by reducing agents.
- 346:350 binding FAD
- 359 binding FAD
- 403 binding FAD
- 535 C→A: Slows the conversion from the dehydrogenase form to the oxidase form; when associated with R-992. Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with R-992 and S-1316.
- 992 C→R: Slows the conversion from the dehydrogenase form to the oxidase form; when associated with A-535. Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with A-535 and S-1316.
- 1316 C→S: Abolishes conversion from the dehydrogenase form to the oxidase form; when associated with A-535 and R-992.
Query Sequence
>WP_157362518.1 NCBI__GCF_000504245.1:WP_157362518.1
MPLLWVLRDKLGITGPKYGCGVGLCRACTSHLNGNEIQPCVVPVSECDGQEVTTIEGLAD
GDELHPVQQAWVDADVGQCGFCQPGQIMAAVALLQRKSKPTDADIDAIENMCRCGTYPRL
RAAIKEIAGIPTDPPGNGEPPKGPPEGKPPNGKGPDGRPGKQPQQGGDSSSAEGEQSGDE
KSGSLFDGIKSAFG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory