SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_157619554.1 NCBI__GCF_000576635.1:WP_157619554.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3pmgA Structure of rabbit muscle phosphoglucomutase at 2.4 angstroms resolution. Use of freezing point depressant and reduced temperature to enhance diffractivity (see paper)
53% identity, 99% coverage: 4:543/543 of query aligns to 4:561/561 of 3pmgA

query
sites
3pmgA

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active site: R22 (= R22), S116 (= S113), H117 (= H114), K129 (= K126), D287 (= D277), D289 (= D279), D291 (= D281), R292 (= R282), G379 (= G367), K388 (= K376)
binding magnesium ion: S116 (= S113), D287 (= D277), D289 (= D279), D291 (= D281)

1c4gA Phosphoglucomutase vanadate based transition state analog complex
53% identity, 99% coverage: 4:543/543 of query aligns to 4:561/561 of 1c4gA

query
sites
1c4gA

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active site: R22 (= R22), S116 (= S113), H117 (= H114), K129 (= K126), D287 (= D277), D289 (= D279), D291 (= D281), R292 (= R282), G379 (= G367), K388 (= K376)
binding cobalt (ii) ion: S116 (= S113), D287 (= D277), D289 (= D279), D291 (= D281)
binding alpha-d-glucose-1-phosphate-6-vanadate: R22 (= R22), S116 (= S113), H117 (= H114), K129 (= K126), R292 (= R282), E375 (= E363), S377 (= S365), K388 (= K376), R514 (= R496)

1c47A Binding driven structural changes in crystaline phosphoglucomutase associated with chemical reaction
53% identity, 99% coverage: 4:543/543 of query aligns to 4:561/561 of 1c47A

query
sites
1c47A

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K

A

Q

-

Q

-

F

-

D

-

L

-

E

-

N

R

K

I

F

G

K

E

P

M

F

V

T

V

V

E

E

I

I

I

V

D

D

P

S

V

V

A

E

D

A

Y

Y

A

A

A

T

L

M

M

L

E

R

R

N

L

I

F

F

D

D

F

F

N

N

R

A

I

L

R

K

A

E

L

L

F

L

R

S

G

G

G

P

F

N

R

R

M

L

T

K

F

I

-

R

-

I

D

D

A

A

L

M

S

H

A

G

V

V

T

V

G

G

P

P

Y

Y

A

V

H

K

E

K

I

I

L

L

E

C

K

E

R

E

L

L

G

G

A

A

P

P

A

A

G

N

T

S

V

A

L

V

N

N

G

C

T

V

P

P

L

L

P

E

D

D

F

F

G

G

H

H

P

P

D

D

P

P

N

N

P

L

V

T

H

Y

A

A

H

A

D

D

L

L

V

V

A

E

A

T

M

M

T

K

G

S

A

G

N

E

P

H

P

-

D

D

F

F

G

G

A

A

S

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

N

N

M

M

I

I

M

L

G

G

P

K

N

H

-

G

L

F

F

F

I

V

T

N

P

P

S

S

D

D

S

S

L

V

A

A

V

V

L

I

A

A

N

N

A

I

H

F

L

S

A

I

P

P

G

Y

Y

F

A

Q

-

Q

A

T

G

G

L

V

K

R

G

G

V

F

A

A

R

R

S

S

M

M

P

P

T

T

S

S

R

G

A

A

A

L

D

D

R

R

V

V

A

A

A

N

K

A

L

T

G

K

I

I

P

A

G

L

F

Y

E

E

T

T

P

P

T

T

G

G

W

W

K

K

F

F

G

G

N

N

L

L

L

M

D

D

A

A

G

S

M

K

A

L

T

S

L

L

C

C

G

G

E
|
E

E

E

S

S

A

F

G
|
G

T

T

G

G

S

S

D

D

H

H

V

I

R

R

E

E

K
|
K

D

D

G

G

L

L

W

W

A

A

V

V

L

L

L

A

W

W

L

L

N

S

I

I

L

L

A

A

V

T

R

R

G

K

E

Q

P

S

I

V

G

E

A

D

I

I

M

L

T

K

G

D

H

H

W

W

R

H

E

K

Y

F

G

G

R

R

D

N

Y

F

T

T

R

R

H

Y

D

D

Y

Y

E

E

D

E

V

V

D

E

T

A

A

E

T

G

A

A

D

T

A

K

L

M

M

M

A

K

D

D

L

L

R

E

A

A

N

L

L

M

-

F

-

D

P

R

T

S

L

F

P

V

G

G

M

K

A

Q

F

F

G

S

G

A

T

N

-

D

-

K

-

V

-

Y

T

T

V

V

D

E

A

K

A

A

D

D

D

N

F

F

E

E

Y

Y

R

H

D

D

P

P

V

V

D

D

G

G

S

S

V

V

S

S

S

K

R

N

Q

Q

G

G

V

L

R

R

V

L

T

I

F

F

A

A

D

D

G

G

S

S

R

R

V

I

Y

F

R

R

L

L

S

S

G

G

T

T

G

G

T

S

G

A

G

G

A

A

T

T

L

I

R

R

V

L

Y

Y

M

I

E

D

R

S

Y

Y

E

E

P

K

D

D

Q

N

A

A

R

K

Q

I

D

N

Q

Q

D

D

A

P

Q

Q

E

V

A

M

L

L

S

A

G

P

L

L

I

I

A

S

I

I

A

A

D

L

Q

K

V

V

A

S

G

Q

I

L

R

Q

S

E

R

R

S

T

G

G

R

R

D

T

K

A

P

P

S

T

V

V

I

I

T

T

active site: R22 (= R22), S116 (= S113), H117 (= H114), K129 (= K126), D287 (= D277), D289 (= D279), D291 (= D281), R292 (= R282), G379 (= G367), K388 (= K376)
binding 1,6-di-O-phosphono-alpha-D-glucopyranose: R22 (= R22), S116 (= S113), D291 (= D281), R292 (= R282), E375 (= E363), K388 (= K376)

P00949 Phosphoglucomutase-1; PGM 1; Glucose phosphomutase 1; EC 5.4.2.2 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
53% identity, 99% coverage: 4:543/543 of query aligns to 5:562/562 of P00949

query
sites
P00949

V

V

I

T

V

V

N

K

T

T

T

K

P

A

F

Y

N

P

D

D

Q

Q

K

K

P

P

G

G

T

T

S

S

G

G

L

L

R
|
R

K

K

K

R

V

V

T

K

V

V

Y

F

-

Q

S

S

Q

S

P

T

H

N

Y

Y

V

A

A

E

N

N

F

F

V

I

Q

Q

S

S

I

I

F

I

D

S

V

T

M

V

D

E

G

P

I

A

A

Q

G

R

Q

Q

-

E

-

A

T

T

V

L

V

V

I

V

G

G

D

D

G

G

R

R

F

F

Y

Y

N

M

A

K

E

E

A

A

I

I

Q

Q

I

L

I

I

L

V

R

R

M

I

A

A

G

A

N

N

G

G

I

I

A

G

R

R

V

L

L

V

V

I

G

G

R

Q

G

N

G

G

I

I

L

L

S

S

T

T

P

P

A

A

A

V

S

S

N

C

V

I

I

I

R

R

Q

K

Y

I

E

K

A

A

F

I

G

G

I

I

L

L

S

T

A

A

S
|
S

H

H

N

N

P

P

G

G

P

P

D

N

G

G

D

D

F

F

G

G

I

I

K

K

Y

F

N

N

I

I

G

S

N

N

G

G

P

P

A

A

P

P

E

E

K

A

I

I

T

T

D

D

A

K

I

I

H

F

R

Q

R

I

T

S

G

K

E

T

I

I

S

E

E

E

Y

Y

R

A

L

I

I

C

E

P

A

D

P

L

D

K

I

V

D

D

L

L

D

G

S

V

L

L

G

G

K

K

A

Q

-

Q

-

F

-

D

-

L

-

E

-

N

R

K

I

F

G

K

E

P

M

F

V

T

V

V

E

E

I

I

I

V

D

D

P

S

V

V

A

E

D

A

Y

Y

A

A

A

T

L

M

M

L

E

R

R

N

L

I

F

F

D

D

F

F

N

N

R

A

I

L

R

K

A

E

L

L

F

L

R

S

G

G

G

P

F

N

R

R

M

L

T

K

F

I

-

R

-

I

D

D

A

A

L

M

S

H

A

G

V

V

T

V

G

G

P

P

Y

Y

A

V

H

K

E

K

I

I

L

L

E

C

K

E

R

E

L

L

G

G

A

A

P

P

A

A

G

N

T

S

V

A

L

V

N

N

G

C

T

V

P

P

L

L

P

E

D

D

F

F

G

G

H

H

P

P

D

D

P

P

N

N

P

L

V

T

H

Y

A

A

H

A

D

D

L

L

V

V

A

E

A

T

M

M

T

K

G

S

A

G

N

E

P

H

P

-

D

D

F

F

G

G

A

A

S

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

N

N

M

M

I

I

M

L

G

G

P

K

N

H

-

G

L

F

F

F

I

V

T

N

P

P

S

S

D

D

S

S

L

V

A

A

V

V

L

I

A

A

N

N

A

I

H

F

L

S

A

I

P

P

G

Y

Y

F

A

Q

-

Q

A

T

G

G

L

V

K

R

G

G

V

F

A

A

R

R

S

S

M

M

P

P

T

T

S

S

R

G

A

A

A

L

D

D

R

R

V

V

A

A

A

N

K

A

L

T

G

K

I

I

P

A

G

L

F

Y

E

E

T

T

P

P

T
|
T

G

G

W

W

K

K

F

F

G

G

N

N

L

L

L

M

D

D

A

A

G

S

M

K

A

L

T

S

L

L

C

C

G

G

E
|
E

E

E

S
|
S

A

F

G

G

T

T

G

G

S

S

D

D

H

H

V

I

R

R

E

E

K
|
K

D

D

G

G

L

L

W

W

A

A

V

V

L

L

L

A

W

W

L

L

N

S

I

I

L

L

A

A

V

T

R

R

G

K

E

Q

P

S

I

V

G

E

A

D

I

I

M

L

T

K

G

D

H

H

W

W

R

H

E

K

Y

F

G

G

R

R

D

N

Y

F

T

T

R

R

H

Y

D

D

Y

Y

E

E

D

E

V

V

D

E

T

A

A

E

T

G

A

A

D

T

A

K

L

M

M

M

A

K

D

D

L

L

R

E

A

A

N

L

L

M

-

F

-

D

P

R

T

S

L

F

P

V

G

G

M

K

A

Q

F

F

G

S

G

A

T

N

-

D

-

K

-

V

-

Y

T

T

V

V

D

E

A

K

A

A

D

D

D

N

F

F

E

E

Y

Y

R

H

D

D

P

P

V

V

D

D

G

G

S

S

V

V

S

S

S

K

R

N

Q

Q

G

G

V

L

R

R

V

L

T

I

F

F

A

A

D

D

G

G

S

S

R

R

V

I

Y

F

R

R

L

L

S

S

G

G

T

T

G

G

T

S

G

A

G

G

A

A

T

T

L

I

R

R

V

L

Y

Y

M

I

E

D

R

S

Y

Y

E

E

P

K

D

D

Q

N

A

A

R

K

Q

I

D

N

Q

Q

D

D

A

P

Q

Q

E

V

A

M

L

L

S

A

G

P

L

L

I

I

A

S

I

I

A

A

D

L

Q

K

V

V

A

S

G

Q

I

L

R

Q

S

E

R

R

S

T

G

G

R

R

D

T

K

A

P

P

S

T

V

V

I

I

T

T

R23 (= R22) binding alpha-D-glucose 1,6-bisphosphate
S117 (= S113) active site, Phosphoserine intermediate; binding alpha-D-glucose 1,6-bisphosphate; binding via phosphate group; modified: Phosphoserine
D288 (= D277) binding Mg(2+)
D290 (= D279) binding Mg(2+)
D292 (= D281) binding alpha-D-glucose 1,6-bisphosphate; binding Mg(2+)
R293 (= R282) binding alpha-D-glucose 1,6-bisphosphate
T357 (= T344) binding alpha-D-glucose 1,6-bisphosphate
E376 (= E363) binding alpha-D-glucose 1,6-bisphosphate
S378 (= S365) binding alpha-D-glucose 1,6-bisphosphate
K389 (= K376) binding alpha-D-glucose 1,6-bisphosphate

P36871 Phosphoglucomutase-1; PGM 1; Glucose phosphomutase 1; EC 5.4.2.2 from Homo sapiens (Human) (see 11 papers)
53% identity, 99% coverage: 4:543/543 of query aligns to 5:562/562 of P36871

query
sites
P36871

V

V

I

T

V

V

N

K

T

T

T

Q

P

A

F

Y

N

Q

D

D

Q

Q

K

K

P

P

G

G

T
|
T

S

S

G

G

L

L

R

R

K

K

K

R

V

V

T

K

V

V

Y

F

-

Q

S

S

Q

S

P

A

H

N

Y

Y

V

A

A

E

N
|
N

F

F

V

I

Q
|
Q

S

S

I

I

F

I

D

S

V

T

M

V

D

E

G

P

I

A

A

Q

G

R

Q

Q

-

E

-

A

T

T

V

L

V

V

I

V

G

G

D
|
D

G

G

R

R

F

F

Y

Y

N

M

A
x
K

E

E

A

A

I

I

Q

Q

I

L

I

I

L

A

R

R

M

I

A

A

G

A

N

N

G

G

I

I

A

G

R

R

V

L

L

V

V

I

G

G

R

Q

G

N

G

G

I

I

L

L

S

S

T

T

P

P

A

A

A

V

S

S

N

C

V

I

I

I

R

R

Q

K

Y

I

E

K

A

A

F

I

G

G

I

I

L

L

S
x
T

A

A

S
|
S

H

H

N

N

P

P

G
|
G

G

G

P

P

D

N

G

G

D

D

F

F

G

G

I

I

K

K

Y

F

N

N

I

I

G

S

N

N

G

G

P

P

A

A

P

P

E

E

K

A

I

I

T

T

D

D

A

K

I

I

H

F

R

Q

R

I

T

S

G

K

E

T

I

I

S

E

E

E

Y

Y

R

A

L

V

I

C

E

P

A

D

P

L

D

K

I

V

D

D

L

L

D

G

S

V

L

L

G

G

K

K

A

Q

-

Q

-

F

-

D

-

L

-

E

-

N

R

K

I

F

G

K

E

P

M

F

V

T

V

V

E

E

I

I

I

V

D

D

P

S

V

V

A

E

D

A

Y

Y

A

A

A

T

L

M

M

L

E

R

R

S

L

I

F

F

D

D

F

F

N

S

R

A

I

L

R

K

A

E

L

L

F

L

R

S

G

G

G

P

F

N

R

R

M

L

T

K

F

I

-
x
R

-

I

D

D

A

A

L

M

S

H

A

G

V

V

T

V

G

G

P

P

Y

Y

A

V

H

K

E

K

I

I

L

L

E

C

K

E

R

E

L

L

G

G

A

A

P

P

A

A

G

N

T

S

V

A

L

V

N

N

G

C

T

V

P

P

L

L

P

E

D

D

F

F

G

G

H

H

P

P

D
|
D

P

P

N

N

P

L

V

T

H

Y

A

A

H

A

D

D

L

L

V

V

A

E

A

T

M

M

T

K

G

-

A

S

N

G

P

E

P

H

D

D

F

F

G

G

A

A

S

F

D
|
D

G

G

D
|
D

G
|
G

D
|
D

R

R

N

N

M

M

I

I

M

L

G

G

P

K

N

H

-

G

L

F

F

F

I

V

T

N

P

P

S

S

D

D

S

S

L

V

A

A

V

V

L

I

A

A

N

N

A

I

H

F

L

S

A

I

P

P

G

Y

Y

F

A

Q

-

Q

A

T

G

G

L

V

K

R

G
|
G

V

F

A

A

R

R

S

S

M

M

P

P

T

T

S

S

R

G

A

A

A

L

D

D

R

R

V

V

A

A

A

S

K

A

L

T

G

K

I

I

P

A

G

L

F

Y

E

E

T

T

P

P

T

T

G

G

W

W

K

K

F

F

G

G

N

N

L

L

L

M

D

D

A

A

G

S

M

K

A

L

T

S

L

L

C

C

G

G

E

E

E
|
E

S

S

A

F

G

G

T

T

G

G

S

S

D

D

H

H

V

I

R

R

E
|
E

K

K

D

D

G

G

L

L

W

W

A

A

V

V

L

L

L

A

W

W

L

L

N

S

I

I

L

L

A

A

V

T

R

R

G

K

E

Q

P

S

I

V

G

E

A

D

I

I

M

L

T

K

G

D

H

H

W

W

R

Q

E

K

Y
|
Y

G

G

R

R

D

N

Y

F

T

T

R

R

H

Y

D

D

Y

Y

E

E

D

E

V

V

D

E

T

A

A

E

T

G

A

A

D

N

A

K

L

M

M

M

A

K

D

D

L

L

R

E

A

A

N

L

L

M

-

F

-

D

P

R

T

S

L

F

P

V

G

G

M

K

A

Q

F

F

G

S

G

A

T

N

-

D

-

K

-

V

-

Y

T
|
T

V

V

D

E

A

K

A

A

D

D

D

N

F

F

E

E

Y

Y

R

S

D

D

P

P

V

V

D

D

G

G

S

S

V

I

S

S

S

R

R

N

Q

Q

G

G

V

L

R

R

V

L

T

I

F

F

A

T

D

D

G

G

S

S

R

R

V

I

V

V

Y

F

R

R

L

L

S

S

G

G

T

T

G

G

T

S

G

A

G

G

A

A

T

T

L

I

R

R

V
x
L

Y

Y

M

I

E

D

R

S

Y

Y

E

E

P

K

D

D

Q

V

A

A

R

K

Q

I

D

N

Q

Q

D

D

A

P

Q

Q

E

V

A

M

L

L

S

A

G

P

L

L

I

I

A

S

I

I

A

A

D

L

Q

K

V

V

A

S

G

Q

I

L

R

Q

S

E

R

R

S

T

G

G

R

R

D

T

K

A

P

P

S

T

V

V

I

I

T

T

T19 (= T18) to A: in CDG1T; strongly reduces phosphoglucomutase activity; dbSNP:rs1320810473
N38 (= N36) to Y: in CDG1T; strongly reduces solubility; increases aggregation; dbSNP:rs587777402
Q41 (= Q39) to R: in CDG1T; reduces solubility; increases aggregation; dbSNP:rs1300651770
D62 (= D58) to H: in CDG1T; reduces solubility; reduces strongly phosphoglucomutase activity; dbSNP:rs587777403
K68 (≠ A64) to M: in allele PGM1*7+, allele PGM1*7-, allele PGM1*3+ and allele PGM1*3-; phosphoglucomutase activity is similar to wild-type; dbSNP:rs200390982
T115 (≠ S111) to A: in CDG1T; reduces mildly phosphoglucomutase activity; dbSNP:rs121918371
S117 (= S113) active site, Phosphoserine intermediate; binding via phosphate groupe; modified: Phosphoserine
G121 (= G117) to R: in CDG1T; there is 7% enzyme residual phosphoglucomutase activity; dbSNP:rs398122912
R221 (vs. gap) to C: in allele PGM1*2+, allele PGM1*2-, allele PGM1*3+ and allele PGM1*3-; phosphoglucomutase activity is similar to wild-type; dbSNP:rs1126728
D263 (= D251) to G: in CDG1T; strongly reduces phosphoglucomutase activity; dbSNP:rs1465877146; to Y: in CDG1T; strongly reduces phosphoglucomutase activity; dbSNP:rs587777404
D288 (= D277) binding Mg(2+)
D290 (= D279) binding Mg(2+)
G291 (= G280) to R: in CDG1T; strongly reduces phosphoglucomutase activity; dbSNP:rs772768778
D292 (= D281) binding Mg(2+)
G330 (= G317) to R: in CDG1T; decreases mildly solubility; dbSNP:rs777164338
E377 (= E364) to K: in CDG1T; decreases strongly solubility
E388 (= E375) to K: in CDG1T; decreases strongly solubility; dbSNP:rs1301021797
Y420 (= Y407) to H: in allele PGM1*1-, allele PGM1*2-, allele PGM1*3- and allele PGM1*7-; phosphoglucomutase activity is similar to wild-type; dbSNP:rs11208257
T467 (= T448) modified: Phosphothreonine; by PAK1
L516 (≠ V497) to P: in CDG1T; decreases strongly solubility; dbSNP:rs587777401

6snoA Crystal structures of human pgm1 isoform 2 (see paper)
53% identity, 99% coverage: 8:543/543 of query aligns to 22:573/573 of 6snoA

query
sites
6snoA

T

T

T

A

P

P

F

Y

N

H

D

D

Q

Q

K

K

P

P

G

G

T

T

S

S

G

G

L

L

R
|
R

K

K

V

T

T

Y

V

Y

Y

F

S

E

Q

E

-

K

P

P

H

C

Y

Y

V

L

A

E

N

N

F

F

V

I

Q

Q

S

S

I

I

F

F

D

F

V

S

M

I

D

D

-

L

-

K

G

D

I

R

A

Q

G

G

Q

S

T

S

V

L

V

V

I

V

G

G

D

D

G

G

R

R

F

Y

Y

F

N

N

A

K

E

S

A

A

I

I

Q

E

I

T

I

I

L

V

R

Q

M

M

A

A

G

A

N

N

G

G

I

I

A

G

R

R

V

L

L

V

V

I

G

G

R

Q

G

N

G

G

I

I

L

L

S

S

T

T

P

P

A

A

A

V

S

S

N

C

V

I

I

I

R

R

Q

K

Y

I

E

K

A

A

F

I

G

G

I

I

L

L

S

T

A

A

S
|
S

H
|
H

N

N

P

P

G

G

P

P

D

N

G

G

D

D

F

F

G

G

I

I

K
|
K

Y

F

N

N

I

I

G

S

N

N

G

G

P

P

A

A

P

P

E

E

K

A

I

I

T

T

D

D

A

K

I

I

H

F

R

Q

R

I

T

S

G

K

E

T

I

I

S

E

E

E

Y

Y

R

A

L

V

I

C

E

P

A

D

P

L

D

K

I

V

D

D

L

L

D

G

S

V

L

L

G

G

K

K

A

Q

-

Q

-

F

-

D

-

L

-

E

-

N

R

K

I

F

G

K

E

P

M

F

V

T

V

V

E

E

I

I

I

V

D

D

P

S

V

V

A

E

D

A

Y

Y

A

A

A

T

L

M

M

L

E

R

R

S

L

I

F

F

D

D

F

F

N

S

R

A

I

L

R

K

A

E

L

L

F

L

R

S

G

G

G

P

F

N

R

R

M

L

T

K

F

I

-

R

-

I

D

D

A

A

L

M

S

H

A

G

V

V

T

V

G

G

P

P

Y

Y

A

V

H

K

E

K

I

I

L

L

E

C

K

E

R

E

L

L

G

G

A

A

P

P

A

A

G

N

T

S

V

A

L

V

N

N

G

C

T

V

P

P

L

L

P

E

D

D

F

F

G

G

H

H

P

P

D

D

P

P

N

N

P

L

V

T

H

Y

A

A

H

A

D

D

L

L

V

V

A

E

A

T

M

M

T

K

G

S

A

G

N

E

P

H

P

-

D

D

F

F

G

G

A

A

S

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

N

N

M

M

I

I

M

L

G

G

P

K

N

H

-

G

L

F

F

F

I

V

T

N

P

P

S

S

D

D

S

S

L

V

A

A

V

V

L

I

A

A

N

N

A

I

H

F

L

S

A

I

P

P

G

Y

Y

F

A

Q

-

Q

A

T

G

G

L

V

K

R

G

G

V

F

A

A

R

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S

S

M

M

P

P

T

T

S

S

R

G

A

A

A

L

D

D

R

R

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V

A

A

A

S

K

A

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T

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K

I

I

P

A

G

L

F

Y

E

E

T

T

P

P

T

T

G

G

W

W

K

K

F

F

G

G

N

N

L

L

L

M

D

D

A

A

G

S

M

K

A

L

T

S

L

L

C

C

G

G

E
|
E

E

E

S
|
S

A

F

G
|
G

T

T

G

G

S

S

D

D

H

H

V

I

R

R

E

E

K

K

D

D

G

G

L

L

W

W

A

A

V

V

L

L

L

A

W

W

L

L

N

S

I

I

L

L

A

A

V

T

R

R

G

K

E

Q

P

S

I

V

G

E

A

D

I

I

M

L

T

K

G

D

H

H

W

W

R

Q

E

K

Y

Y

G

G

R

R

D

N

Y

F

T

T

R

R

H

Y

D

D

Y

Y

E

E

D

E

V

V

D

E

T

A

A

E

T

G

A

A

D

N

A

K

L

M

M

M

A

K

D

D

L

L

R

E

A

A

N

L

L

M

-

F

-

D

P

R

T

S

L

F

P

V

G

G

M

K

A

Q

F

F

G

S

G

A

T

N

-

V

-

Y

T

T

V

V

D

E

A

K

A

A

D

D

D

N

F

F

E

E

Y

Y

R

S

D

D

P

P

V

V

D

D

G

G

S

S

V

I

S

S

S

R

R

N

Q

Q

G

G

V

L

R

R

V

L

T

I

F

F

A

T

D

D

G

G

S

S

R

R

V

I

V

V

Y

F

R
|
R

L

L

S
|
S

G
|
G

T
|
T

G

G

T

S

G

A

G

G

A

A

T

T

L

I

R
|
R

V

L

Y

Y

M

I

E

D

R

S

Y

Y

E

E

P

K

D

D

Q

V

A

A

R

K

Q

I

D

N

Q

Q

D

D

A

P

Q

Q

E

V

A

M

L

L

S

A

G

P

L

L

I

I

A

S

I

I

A

A

D

L

Q

K

V

V

A

S

G

Q

I

L

R

Q

S

E

R

R

S

T

G

G

R

R

D

T

K

A

P

P

S

T

V

V

I

I

T

T

active site: R36 (= R22), S130 (= S113), H131 (= H114), K143 (= K126), D301 (= D277), D303 (= D279), D305 (= D281), R306 (= R282), G393 (= G367)
binding 1-O-phosphono-alpha-D-glucopyranose: S130 (= S113), E389 (= E363), S391 (= S365), R514 (= R484), S516 (= S486), G517 (= G487), T518 (= T488), R526 (= R496)
binding zinc ion: S130 (= S113), D301 (= D277), D303 (= D279), D305 (= D281)

6snqA Crystal structures of human pgm1 isoform 2 (see paper)
53% identity, 99% coverage: 8:543/543 of query aligns to 22:566/566 of 6snqA

query
sites
6snqA

T

T

T

A

P

P

F

Y

N

H

D

D

Q

Q

K

K

P

P

G

G

T

T

S

S

G

G

L

L

R
|
R

K

K

V

T

T

Y

V

Y

Y

F

S

E

Q

E

-

K

P

P

H

C

Y

Y

V

L

A

E

N

N

F

F

V

I

Q

Q

S

S

I

I

F

F

D

F

V

S

M

I

D

D

-

L

-

K

G

D

I

R

A

Q

G

G

Q

S

T

S

V

L

V

V

I

V

G

G

D

D

G

G

R

R

F

Y

Y

F

N

N

A

K

E

S

A

A

I

I

Q

E

I

T

I

I

L

V

R

Q

M

M

A

A

G

A

N

N

G

G

I

I

A

G

R

R

V

L

L

V

V

I

G

G

R

Q

G

N

G

G

I

I

L

L

S

S

T

T

P

P

A

A

A

V

S

S

N

C

V

I

I

I

R

R

Q

K

Y

I

E

K

A

A

F

I

G

G

I

I

L

L

S

T

A

A

S
|
S

H
|
H

N

N

P

P

G

G

P

P

D

N

G

G

D

D

F

F

G

G

I

I

K
|
K

Y

F

N

N

I

I

G

S

N

N

G

G

P

P

A

A

P

P

E

E

K

A

I

I

T

T

D

D

A

K

I

I

H

F

R

Q

R

I

T

S

G

K

E

T

I

I

S

E

E

E

Y

Y

R

A

L

V

I

C

E

P

A

D

P

L

D

K

I

V

D

D

L

L

D

G

S

V

L

L

G

G

K

K

A

Q

-

Q

-

F

-

D

-

L

-

E

-

N

R

K

I

F

G

K

E

P

M

F

V

T

V

V

E

E

I

I

I

V

D

D

P

S

V

V

A

E

D

A

Y

Y

A

A

A

T

L

M

M

L

E

R

R

S

L

I

F

F

D

D

F

F

N

S

R

A

I

L

R

K

A

E

L

L

F

L

R

S

G

G

G

P

F

N

R

R

M

L

T

K

F

I

-

R

-

I

D

D

A

A

L

M

S

H

A

G

V

V

T

V

G

G

P

P

Y

Y

A

V

H

K

E

K

I

I

L

L

E

C

K

E

R

E

L

L

G

G

A

A

P

P

A

A

G

N

T

S

V

A

L

V

N

N

G

C

T

V

P

P

L

L

P

E

D

D

F

F

G

G

H

H

P

P

D

D

P

P

N

N

P

L

V

T

H

Y

A

A

H

A

D

D

L

L

V

V

A

E

A

T

M

M

T

K

G

S

A

G

N

E

P

H

P

-

D

D

F

F

G

G

A

A

S

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

N

N

M

M

I

I

M

L

G

G

P

K

N

H

-

G

L

F

F

F

I

V

T

N

P

P

S

S

D

D

S

S

L

V

A

A

V

V

L

I

A

A

N

N

A

I

H

F

L

S

A

I

P

P

G

Y

Y

F

A

Q

-

Q

A

T

G

G

L

V

K

R

G

G

V

F

A

A

R

R

S

S

M

M

P

P

T

T

S

S

R

G

A

A

A

L

D

D

R

R

V

V

A

A

A

S

K

A

L

T

G

K

I

I

P

A

G

L

F

Y

E

E

T

T

P

P

T
|
T

G
|
G

W

W

K

K

F

F

G

G

N

N

L

L

L

M

D

D

A

A

G

S

M

K

A

L

T

S

L

L

C

C

G

G

E
|
E

E

E

S
|
S

A

F

G
|
G

T

T

G

G

S

S

D

D

H

H

V

I

R

R

E

E

K

K

D

D

G

G

L

L

W

W

A

A

V

V

L

L

L

A

W

W

L

L

N

S

I

I

L

L

A

A

V

T

R

R

G

K

E

Q

P

S

I

V

G

E

A

D

I

I

M

L

T

K

G

D

H

H

W

W

R

Q

E

K

Y

Y

G

G

R

R

D

N

Y

F

T

T

R

R

H

Y

D

D

Y

Y

E

E

D

E

V

V

D

E

T

A

A

E

T

G

A

A

D

N

A

K

L

M

M

M

A

K

D

D

L

L

R

E

A

A

N

L

L

M

-

F

-

D

P

R

T

S

L

F

P

V

G

G

M

K

A

Q

F

F

G

S

G

V

T

Y

T

T

V

V

D

E

A

K

A

A

D

D

D

N

F

F

E

E

Y

Y

R

S

D

D

P

P

V

V

D

D

G

G

S

S

V

I

S

S

S

R

R

N

Q

Q

G

G

V

L

R

R

V

L

T

I

F

F

A

T

D

D

G

G

S

S

R

R

V

I

V

V

Y

F

R
|
R

L

L

S
|
S

G

-

T

-

G

-

T

-

G

-

G

G

A

A

T

T

L

I

R
|
R

V

L

Y

Y

M

I

E

D

R

S

Y

Y

E

E

P

K

D

D

Q

V

A

A

R

K

Q

I

D

N

Q

Q

D

D

A

P

Q

Q

E

V

A

M

L

L

S

A

G

P

L

L

I

I

A

S

I

I

A

A

D

L

Q

K

V

V

A

S

G

Q

I

L

R

Q

S

E

R

R

S

T

G

G

R

R

D

T

K

A

P

P

S

T

V

V

I

I

T

T

active site: R36 (= R22), S130 (= S113), H131 (= H114), K143 (= K126), D301 (= D277), D303 (= D279), D305 (= D281), R306 (= R282), G393 (= G367)
binding 6-O-phosphono-alpha-D-glucopyranose: S130 (= S113), T370 (= T344), G371 (= G345), E389 (= E363), S391 (= S365), R512 (= R484), S514 (= S486), R519 (= R496)
binding zinc ion: S130 (= S113), D301 (= D277), D303 (= D279), D305 (= D281)

5jn5A Crystal structure of the d263y missense variant of human pgm1 (see paper)
53% identity, 99% coverage: 4:543/543 of query aligns to 6:559/559 of 5jn5A

query
sites
5jn5A

V

V

I

T

V

V

N

K

T

T

T

Q

P

A

F

Y

N

Q

D

D

Q

Q

K

K

P

P

G

G

T

T

S

S

G

G

L

L

R
|
R

K

K

K

R

V

V

T

K

V

V

Y

F

-

Q

S

S

Q

S

P

A

H

N

Y

Y

V

A

A

E

N

N

F

F

V

I

Q

Q

S

S

I

I

F

I

D

S

V

T

M

V

D

E

G

P

I

A

A

Q

G

R

Q

Q

-

E

-

A

T

T

V

L

V

V

I

V

G

G

D

D

G

G

R

R

F

F

Y

Y

N

M

A

K

E

E

A

A

I

I

Q

Q

I

L

I

I

L

A

R

R

M

I

A

A

G

A

N

N

G

G

I

I

A

G

R

R

V

L

L

V

V

I

G

G

R

Q

G

N

G

G

I

I

L

L

S

S

T

T

P

P

A

A

A

V

S

S

N

C

V

I

I

I

R

R

Q

K

Y

I

E

K

A

A

F

I

G

G

I

I

L

L

S

T

A

A

S
|
S

H
|
H

N

N

P

P

G

G

P

P

D

N

G

G

D

D

F

F

G

G

I

I

K
|
K

Y

F

N

N

I

I

G

S

N

N

G

G

P

P

A

A

P

P

E

E

K

A

I

I

T

T

D

D

A

K

I

I

H

F

R

Q

R

I

T

S

G

K

E

T

I

I

S

E

E

E

Y

Y

R

A

L

V

I

C

E

P

A

D

P

L

D

K

I

V

D

D

L

L

D

G

S

V

L

L

G

G

K

K

A

Q

-

Q

-

F

-

D

-

L

-

E

-

N

R

K

I

F

G

K

E

P

M

F

V

T

V

V

E

E

I

I

I

V

D

D

P

S

V

V

A

E

D

A

Y

Y

A

A

A

T

L

M

M

L

E

R

R

S

L

I

F

F

D

D

F

F

N

S

R

A

I

L

R

K

A

E

L

L

F

L

R

S

G

G

G

P

F

N

R

R

M

L

T

K

F

I

-

R

-

I

D

D

A

A

L

M

S

H

A

G

V

V

T

V

G

G

P

P

Y

Y

A

V

H

K

E

K

I

I

L

L

E

C

K

E

R

E

L

L

G

G

A

A

P

P

A

A

G

N

T

S

V

A

L

V

N

N

G

C

T

V

P

P

L

L

P

E

D

D

F

F

G

G

H

H

P

P

D

Y

P

P

N

N

P

L

V

T

H

Y

A

A

H

A

D

D

L

L

V

V

A

E

A

T

M

M

T

K

G

-

A

S

N

G

P

E

P

H

D

D

F

F

G

G

A

A

S

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

N

N

M

M

I

I

M

L

G

G

P

K

N

H

-

G

L

F

F

F

I

V

T

N

P

P

S

S

D

D

S

S

L

V

A

A

V

V

L

I

A

A

N

N

A

I

H

F

L

S

A

I

P

P

G

Y

Y

F

A

Q

-

Q

A

T

G

G

L

V

K

R

G

G

V

F

A

A

R

R

S

S

M

M

P

P

T

T

S

S

R

G

A

A

A

L

D

D

R

R

V

V

A

A

A

S

K

A

L

T

G

K

I

I

P

A

G

L

F

Y

E

E

T

T

P

P

T

T

G

G

W

W

K

K

F

F

G

G

N

N

L

L

L

M

D

D

A

A

G

S

M

K

A

L

T

S

L

L

C

C

G

G

E

E

S

S

A

F

G
|
G

T

T

G

G

S

S

D

D

H

H

V

I

R

R

E

E

K
|
K

D

D

G

G

L

L

W

W

A

A

V

V

L

L

L

A

W

W

L

L

N

S

I

I

L

L

A

A

V

T

R

R

G

K

E

Q

P

S

I

V

G

E

A

D

I

I

M

L

T

K

G

D

H

H

W

W

R

Q

E

K

Y

Y

G

G

R

R

D

N

Y

F

T

T

R

R

H

Y

D

D

Y

Y

E

E

D

E

V

V

D

E

T

A

A

E

T

G

A

A

D

N

A

K

L

M

M

M

A

K

D

D

L

L

R

E

A

A

N

L

L

M

-

F

-

D

P

R

T

S

L

F

P

V

G

G

M

K

A

Q

F

F

G

S

G

A

T

N

-

D

-

K

-

V

-

Y

T

T

V

V

D

E

A

K

A

A

D

D

D

N

F

F

E

E

Y

Y

R

S

D

D

P

P

V

V

D

D

G

G

S

S

V

I

S

S

S

R

R

N

Q

Q

G

G

V

L

R

R

V

L

T

I

F

F

A

T

D

D

G

G

S

S

R

R

V

I

V

V

Y

F

R

R

L

L

S

S

G

-

T

-

G

-

T

-

G

G

A

A

T

T

L

I

R

R

V

L

Y

Y

M

I

E

D

R

S

Y

Y

E

E

P

K

D

D

Q

V

A

A

R

K

Q

I

D

N

Q

Q

D

D

A

P

Q

Q

E

V

A

M

L

L

S

A

G

P

L

L

I

I

A

S

I

I

A

A

D

L

Q

K

V

V

A

S

G

Q

I

L

R

Q

S

E

R

R

S

T

G

G

R

R

D

T

K

A

P

P

S

T

V

V

I

I

T

T

active site: R24 (= R22), S118 (= S113), H119 (= H114), K131 (= K126), D289 (= D277), D291 (= D279), D293 (= D281), R294 (= R282), G381 (= G367), K390 (= K376)
binding calcium ion: S118 (= S113), D289 (= D277), D291 (= D279), D293 (= D281)

6y8yA Structure of baltic herring (clupea harengus) phosphoglucomutase 5 (pgm5) with bound glucose-1-phosphate (see paper)
50% identity, 99% coverage: 4:543/543 of query aligns to 14:572/572 of 6y8yA

query
sites
6y8yA

V

V

I

T

V

V

N

Q

T

T

P

P

F

F

N

D

D

D

Q

Q

K

K

P

P

G

G

T

T

S

N

G

G

L

L

R

R

K

K

V

T

T

T

V

V

Y

F

-

E

S

S

Q

K

P

K

H

N

Y

Y

V

L

A

Q

N

N

F

Y

V

I

Q

Q

S

S

I

V

F

L

D

S

V

S

M

I

D

D

-

L

-

R

G

D

I

R

A

Q

G

G

Q

C

T

T

V

M

V

V

I

V

G

G

G

S

D

D

G

G

R

R

F

Y

Y

F

N

S

A

R

E

T

A

A

I

I

Q

E

I

V

I

I

L

V

R

Q

M

M

A

A

G

A

N

N

G

G

I

I

A

G

R

R

V

L

L

V

V

I

G

G

R

H<