SitesBLAST
Comparing WP_157619628.1 NCBI__GCF_000576635.1:WP_157619628.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
44% identity, 92% coverage: 19:317/325 of query aligns to 3:304/304 of 1wwkA
- active site: S96 (≠ N107), R230 (= R243), D254 (= D267), E259 (= E272), H278 (= H291)
- binding nicotinamide-adenine-dinucleotide: V100 (= V111), G146 (= G157), F147 (≠ M158), G148 (= G159), R149 (≠ G160), I150 (≠ V161), Y168 (= Y180), D169 (= D181), P170 (= P182), V201 (≠ I214), P202 (= P215), T207 (= T220), T228 (≠ L241), S229 (= S242), D254 (= D267), H278 (= H291), G280 (= G293)
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
39% identity, 82% coverage: 51:317/325 of query aligns to 41:305/305 of 6plfA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
40% identity, 78% coverage: 51:304/325 of query aligns to 40:291/303 of 6plgA
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
40% identity, 78% coverage: 51:304/325 of query aligns to 39:290/297 of 6rj3A
7dkmA Phgdh covalently linked to oridonin (see paper)
40% identity, 78% coverage: 51:304/325 of query aligns to 41:292/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V83), A102 (≠ V111), G148 (= G157), R151 (≠ G160), I152 (≠ V161), Y170 (= Y180), D171 (= D181), P172 (= P182), I173 (≠ F183), H202 (= H213), T203 (≠ I214), P204 (= P215), T209 (= T220), C230 (≠ L241), A231 (≠ S242), R232 (= R243), H279 (= H291), G281 (= G293)
Sites not aligning to the query:
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18, 293
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
40% identity, 78% coverage: 51:304/325 of query aligns to 40:291/301 of 6rj5A
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
40% identity, 78% coverage: 51:304/325 of query aligns to 37:288/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G159), I148 (≠ V161), Y166 (= Y180), D167 (= D181), P168 (= P182), I169 (≠ F183), I170 (≠ A184), H198 (= H213), T199 (≠ I214), L208 (= L223), R228 (= R243)
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
40% identity, 78% coverage: 51:304/325 of query aligns to 39:290/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N107), A100 (≠ V111), R149 (≠ G160), I150 (≠ V161), Y168 (= Y180), D169 (= D181), P170 (= P182), I171 (≠ F183), H200 (= H213), T201 (≠ I214), P202 (= P215), T207 (= T220), C228 (≠ L241), A229 (≠ S242), R230 (= R243), H277 (= H291), G279 (= G293)
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
40% identity, 78% coverage: 51:304/325 of query aligns to 40:291/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ V156), G147 (= G157), L148 (≠ M158), G149 (= G159), R150 (≠ G160), I151 (≠ V161), G152 (= G162), D170 (= D181), H201 (= H213), T202 (≠ I214), P203 (= P215)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
40% identity, 78% coverage: 51:304/325 of query aligns to 40:291/302 of 6rihA
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
40% identity, 78% coverage: 51:304/325 of query aligns to 45:296/533 of O43175
- T78 (≠ V83) binding NAD(+)
- R135 (≠ S140) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ GV 160:161) binding NAD(+)
- D175 (= D181) binding NAD(+)
- T207 (≠ I214) binding NAD(+)
- CAR 234:236 (≠ LSR 241:243) binding NAD(+)
- D260 (= D267) binding NAD(+)
- V261 (= V268) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIGA 291:294) binding NAD(+)
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
40% identity, 78% coverage: 51:304/325 of query aligns to 35:282/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (≠ G160), Y160 (= Y180), D161 (= D181), P162 (= P182), I164 (≠ A184), L179 (= L200), T193 (≠ I214), P194 (= P215), S198 (≠ D219), L202 (= L223)
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
41% identity, 94% coverage: 18:321/325 of query aligns to 3:308/526 of 3dc2A
Sites not aligning to the query:
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
41% identity, 94% coverage: 18:321/325 of query aligns to 4:309/525 of 3ddnB
2eklA Structure of st1218 protein from sulfolobus tokodaii
34% identity, 87% coverage: 21:304/325 of query aligns to 9:295/312 of 2eklA
- active site: S100 (≠ N107), R232 (= R243), D256 (= D267), E261 (= E272), H282 (= H291)
- binding nicotinamide-adenine-dinucleotide: I76 (≠ V83), S100 (≠ N107), G148 (= G157), G150 (= G159), R151 (≠ G160), I152 (≠ V161), Y170 (= Y180), D171 (= D181), I172 (≠ P182), L173 (≠ F183), H202 (= H213), V203 (≠ I214), T204 (≠ P215), I212 (≠ L223), T230 (≠ L241), S231 (= S242), D256 (= D267), G284 (= G293)
8wprA Anabaena mcyi r166a with prebound NAD and malate
34% identity, 84% coverage: 51:324/325 of query aligns to 40:315/320 of 8wprA
- binding nicotinamide-adenine-dinucleotide: S97 (≠ N107), V101 (= V111), G150 (= G159), A151 (≠ G160), I152 (≠ V161), Y171 (= Y180), D172 (= D181), P173 (= P182), Y174 (≠ F183), P205 (≠ I214), E206 (≠ P215), E210 (≠ D219), T211 (= T220), T232 (≠ L241), S233 (= S242), R234 (= R243), H282 (= H291), G285 (≠ A294)
8wpiA Anabaena mcyi with prebound NAD and soaked NADP
34% identity, 84% coverage: 51:322/325 of query aligns to 38:311/318 of 8wpiA
- binding nicotinamide-adenine-dinucleotide: V99 (= V111), G146 (= G157), G148 (= G159), R149 (≠ G160), I150 (≠ V161), Y169 (= Y180), D170 (= D181), P171 (= P182), Y172 (≠ F183), H202 (= H213), P203 (≠ I214), E204 (≠ P215), E208 (≠ D219), T209 (= T220), T230 (≠ L241), S231 (= S242), R232 (= R243), H280 (= H291)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: Y48 (≠ T61), S69 (≠ H81), G70 (= G82), F71 (≠ V83), G72 (= G84), L110 (≠ V122), K113 (≠ R125), F116 (≠ L128), F226 (≠ M237), R232 (= R243), D273 (≠ P284), N274 (≠ G285), V284 (≠ A295), T285 (= T296), G289 (≠ M300)
Sites not aligning to the query:
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
37% identity, 83% coverage: 51:320/325 of query aligns to 43:317/334 of 5aovA
- active site: L100 (≠ N107), R241 (= R243), D265 (= D267), E270 (≠ P273), H288 (= H291)
- binding glyoxylic acid: M52 (≠ R60), L53 (≠ T61), L53 (≠ T61), Y74 (≠ H81), A75 (≠ G82), V76 (= V83), G77 (= G84), R241 (= R243), H288 (= H291)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V83), T104 (≠ V111), F158 (≠ M158), G159 (= G159), R160 (≠ G160), I161 (≠ V161), S180 (≠ D181), R181 (≠ P182), A211 (≠ H213), V212 (≠ I214), P213 (= P215), T218 (= T220), I239 (≠ L241), A240 (≠ S242), R241 (= R243), H288 (= H291), G290 (= G293)
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 96% coverage: 7:319/325 of query aligns to 46:372/466 of P87228
- S87 (≠ R46) modified: Phosphoserine
- S258 (≠ N217) modified: Phosphoserine
2p9eA Crystal structure of g336v mutant of e.Coli phosphoglycerate dehydrogenase (see paper)
33% identity, 85% coverage: 43:319/325 of query aligns to 39:316/406 of 2p9eA
- active site: N104 (= N107), R236 (= R243), D260 (= D267), E265 (= E272), H288 (= H291)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G156 (= G159), H157 (≠ G160), I158 (≠ V161), Y176 (= Y180), D177 (= D181), I178 (≠ P182), H206 (= H213), V207 (≠ I214), P208 (= P215), S212 (≠ D219), A234 (≠ L241), S235 (= S242), R236 (= R243), H288 (= H291), G290 (= G293)
Query Sequence
>WP_157619628.1 NCBI__GCF_000576635.1:WP_157619628.1
MPRPKTRVLKSEMVERSLVYVLDPIAPSAVATLRQSCDVVDHDDKRKGRWLEDADGVIVR
TSKIPRDMIAAAQRLKVIGKHGVGVDNIDLDAARDRGVAVINTPGANSNAVAELAVGLAL
AVARRIALLDRMLRDGAVTSPPPQGIELTGRTVGVVGMGGVGRRTAALFKAAFGSTILAY
DPFAPPEAFAAADAERCASLDELLPRVDVLTLHIPLNRDTEKLIDARRLALMKRDAMVLN
LSRGGVVDETALHDALTNGTIAGAATDVFVQEPPPAGHPLLRLPGFVATPHIGAATAESM
ERMGMGVVGGVVTLLGGGTPDHRVV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory