SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_157892222.1 NCBI__GCF_000058485.1:WP_157892222.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

6cyzA Mycobacterial homoserine kinase thrb in complex with amppnp
36% identity, 41% coverage: 240:412/426 of query aligns to 130:288/295 of 6cyzA

query
sites
6cyzA
M
 
L
L
 
V
R
 
Q
L
 
L
A
 
A
H
 
S
D
 
E
I
 
F
E
 
E
G
 
G
H
 
H
P
 
P
D
 
D
N
 
N
V
 
A
A
 
A
A
 
A
A
 
S
L
 
V
S
 
L
G
 
G
G
 
G
F
 
A
T
 
V
V
 
V
A
 
S
W
 
W
Q
 
S
D
 
E
V
 
T
E
 
T
G
 
P
A
 
I
R
 
Y
C
 
A
-
 
A
L
 
T
R
 
R
V
 
L
D
 
D
P
 
V
F
 
H
A
 
P
E
 
D
L
 
I
R
 
K
P
 
I
V
 
V
V
 
A
F
 
A
V
 
I
P
 
P
T
 
E
V
 
-
R
 
-
Q
 
-
S
 
-
T
 
-
E
 
-
A
 
-
S
 
T
R
 
R
G
 
V
A
 
L
L
 
L
P
 
P
V
 
Q
L
 
A
V
 
V
G
 
T
L
 
H
P
 
V
D
 
D
A
 
A
A
 
R
R
 
F
T
 
N
L
 
I
G
 
S
R
 
R
A
 
V
A
 
A
L
 
L
L
 
L
A
 
T
L
 
V
T
 
A
M
 
L
S
 
T
A
 
A
A
 
-
E
 
-
P
 
-
A
 
-
A
 
-
A
 
-
A
 
-
Q
 
-
R
 
R
A
 
P
R
 
D
T
 
L
L
 
L
F
 
M
S
 
T
A
 
A
T
 
T
E
 
E
D
 
D
L
 
R
L
 
L
H
 
H
Q
 
Q
P
 
P
Y
 
Q
R
 
R
L
 
A
P
 
S
A
 
A
A
 
M
P
 
P
A
 
A
T
 
S
G
 
A
E
 
D
L
 
V
V
 
L
A
 
A
R
 
Y
L
 
L
R
 
R
A
 
S
L
 
Q
G
 
G
V
 
V
P
 
A
A
 
A
T
 
V
L
 
L
S
 
S
G
 
G
S
 
A
G
 
G
P
 
P
S
 
A
V
 
V
L
 
L
A
 
A
L
 
L
A
 
T
V
 
T
G
 
V
G
 
D
E
 
L
Q
 
P
A
 
D
A
 
S
A
 
A
A
 
V
V
 
K
G
 
Y
A
 
A
A
 
E
S
 
D
A
 
Q
E
 
G
F
 
F
S
 
S
V
 
L
A
 
V
P
 
A
L
 
M
S
 
A
V
 
V

Sites not aligning to the query:

1h74B Crystal structure of homoserine kinase complexed with ile (see paper)
28% identity, 32% coverage: 250:387/426 of query aligns to 134:264/296 of 1h74B

query
sites
1h74B
H
|
H
P
 
A
D
|
D
N
|
N
V
 
V
A
 
A
A
 
P
A
 
A
L
 
I
S
 
F
G
 
G
G
 
G
F
 
F
T
 
T
V
 
M
A
 
V
W
 
-
Q
 
T
D
 
N
V
 
Y
E
 
E
G
 
P
A
 
L
R
 
E
C
 
V
L
 
L
R
 
H
V
 
I
D
 
P
P
 
I
F
 
D
A
 
F
E
 
K
L
 
L
R
 
D
P
 
I
V
 
L
V
 
I
F
 
A
V
 
I
P
 
P
T
 
N
V
 
I
R
 
S
Q
 
I
S
 
N
T
|
T
E
 
K
A
 
E
S
 
A
R
|
R
G
 
E
A
 
I
L
 
L
P
 
P
V
 
K
L
 
A
V
 
V
G
 
G
L
 
L
P
 
K
D
 
D
A
 
L
A
 
V
R
 
N
T
 
N
L
 
V
G
 
G
R
 
K
A
 
A
A
 
C
L
 
G
L
 
M
A
 
V
L
 
Y
T
 
-
M
 
-
S
 
-
A
 
-
A
 
-
E
 
-
P
 
-
A
 
-
A
 
A
A
 
L
A
 
Y
Q
 
N
R
 
K
A
 
D
R
 
K
T
 
S
L
 
L
F
 
F
-
 
G
-
 
R
S
 
Y
A
 
M
T
 
M
E
 
S
D
 
D
L
 
K
L
 
V
H
 
I
Q
 
E
P
 
P
Y
 
V
R
|
R
L
 
G
P
 
K
A
 
L
A
 
I
P
 
P
A
 
N
T
 
Y
G
 
F
E
 
K
L
 
I
V
 
K
A
 
E
R
 
E
L
 
V
R
 
K
A
 
D
L
 
K
G
 
V
V
 
Y
P
 
G
A
 
I
T
 
T
L
 
I
S
 
S
G
|
G
S
 
S
G
 
G
P
 
P
S
 
S
V
 
I
L
 
I
A
 
A
L
 
F

Sites not aligning to the query:

1h74A Crystal structure of homoserine kinase complexed with ile (see paper)
28% identity, 32% coverage: 250:387/426 of query aligns to 134:264/296 of 1h74A

query
sites
1h74A
H
|
H
P
 
A
D
 
D
N
 
N
V
 
V
A
 
A
A
 
P
A
 
A
L
 
I
S
 
F
G
 
G
G
 
G
F
 
F
T
 
T
V
 
M
A
 
V
W
 
-
Q
 
T
D
 
N
V
 
Y
E
 
E
G
 
P
A
 
L
R
 
E
C
 
V
L
 
L
R
 
H
V
 
I
D
 
P
P
 
I
F
 
D
A
 
F
E
 
K
L
 
L
R
 
D
P
 
I
V
 
L
V
 
I
F
 
A
V
 
I
P
 
P
T
 
N
V
 
I
R
 
S
Q
 
I
S
 
N
T
|
T
E
 
K
A
 
E
S
 
A
R
|
R
G
 
E
A
 
I
L
 
L
P
 
P
V
 
K
L
 
A
V
 
V
G
 
G
L
 
L
P
 
K
D
 
D
A
 
L
A
 
V
R
 
N
T
 
N
L
 
V
G
 
G
R
 
K
A
 
A
A
 
C
L
 
G
L
 
M
A
 
V
L
 
Y
T
 
-
M
 
-
S
 
-
A
 
-
A
 
-
E
 
-
P
 
-
A
 
-
A
 
A
A
 
L
A
 
Y
Q
 
N
R
 
K
A
 
D
R
 
K
T
 
S
L
 
L
F
 
F
-
 
G
-
 
R
S
 
Y
A
 
M
T
 
M
E
 
S
D
 
D
L
 
K
L
 
V
H
 
I
Q
 
E
P
 
P
Y
 
V
R
|
R
L
 
G
P
 
K
A
 
L
A
 
I
P
 
P
A
 
N
T
 
Y
G
 
F
E
 
K
L
 
I
V
 
K
A
 
E
R
 
E
L
 
V
R
 
K
A
 
D
L
 
K
G
 
V
V
 
Y
P
 
G
A
 
I
T
 
T
L
 
I
S
 
S
G
 
G
S
 
S
G
 
G
P
 
P
S
 
S
V
 
I
L
 
I
A
 
A
L
 
F

Sites not aligning to the query:

1h73A Crystal structure of homoserine kinase complexed with threonine (see paper)
28% identity, 32% coverage: 250:387/426 of query aligns to 134:264/296 of 1h73A

query
sites
1h73A
H
 
H
P
 
A
D
 
D
N
 
N
V
 
V
A
 
A
A
 
P
A
 
A
L
 
I
S
 
F
G
 
G
G
 
G
F
 
F
T
 
T
V
 
M
A
 
V
W
 
-
Q
 
T
D
 
N
V
 
Y
E
 
E
G
 
P
A
 
L
R
 
E
C
 
V
L
 
L
R
 
H
V
 
I
D
 
P
P
 
I
F
 
D
A
 
F
E
 
K
L
 
L
R
 
D
P
 
I
V
 
L
V
 
I
F
 
A
V
 
I
P
 
P
T
 
N
V
 
I
R
 
S
Q
 
I
S
 
N
T
|
T
E
 
K
A
 
E
S
 
A
R
|
R
G
 
E
A
 
I
L
 
L
P
 
P
V
 
K
L
 
A
V
 
V
G
 
G
L
 
L
P
 
K
D
 
D
A
 
L
A
 
V
R
 
N
T
 
N
L
 
V
G
 
G
R
 
K
A
 
A
A
 
C
L
 
G
L
 
M
A
 
V
L
 
Y
T
 
-
M
 
-
S
 
-
A
 
-
A
 
-
E
 
-
P
 
-
A
 
-
A
 
A
A
 
L
A
 
Y
Q
 
N
R
 
K
A
 
D
R
 
K
T
 
S
L
 
L
F
 
F
-
 
G
-
 
R
S
 
Y
A
 
M
T
 
M
E
 
S
D
 
D
L
 
K
L
 
V
H
 
I
Q
 
E
P
 
P
Y
 
V
R
|
R
L
 
G
P
 
K
A
 
L
A
 
I
P
 
P
A
 
N
T
 
Y
G
 
F
E
 
K
L
 
I
V
 
K
A
 
E
R
 
E
L
 
V
R
 
K
A
 
D
L
 
K
G
 
V
V
 
Y
P
 
G
A
 
I
T
 
T
L
 
I
S
 
S
G
 
G
S
 
S
G
 
G
P
 
P
S
 
S
V
 
I
L
 
I
A
 
A
L
 
F

Sites not aligning to the query:

1h72C Crystal structure of homoserine kinase complexed with hse (see paper)
28% identity, 32% coverage: 250:387/426 of query aligns to 134:264/296 of 1h72C

query
sites
1h72C
H
 
H
P
 
A
D
|
D
N
|
N
V
 
V
A
 
A
A
 
P
A
 
A
L
 
I
S
 
F
G
 
G
G
 
G
F
 
F
T
 
T
V
 
M
A
 
V
W
 
-
Q
 
T
D
 
N
V
 
Y
E
 
E
G
 
P
A
 
L
R
 
E
C
 
V
L
 
L
R
 
H
V
 
I
D
 
P
P
 
I
F
 
D
A
 
F
E
 
K
L
 
L
R
 
D
P
 
I
V
 
L
V
 
I
F
 
A
V
 
I
P
 
P
T
 
N
V
 
I
R
 
S
Q
 
I
S
 
N
T
|
T
E
 
K
A
 
E
S
 
A
R
|
R
G
 
E
A
 
I
L
 
L
P
 
P
V
 
K
L
 
A
V
 
V
G
 
G
L
 
L
P
 
K
D
 
D
A
 
L
A
 
V
R
 
N
T
 
N
L
 
V
G
 
G
R
 
K
A
 
A
A
 
C
L
 
G
L
 
M
A
 
V
L
 
Y
T
 
-
M
 
-
S
 
-
A
 
-
A
 
-
E
 
-
P
 
-
A
 
-
A
 
A
A
 
L
A
 
Y
Q
 
N
R
 
K
A
 
D
R
 
K
T
 
S
L
 
L
F
 
F
-
 
G
-
 
R
S
 
Y
A
 
M
T
 
M
E
 
S
D
 
D
L
 
K
L
 
V
H
 
I
Q
 
E
P
 
P
Y
 
V
R
|
R
L
 
G
P
 
K
A
 
L
A
 
I
P
 
P
A
 
N
T
 
Y
G
 
F
E
 
K
L
 
I
V
 
K
A
 
E
R
 
E
L
 
V
R
 
K
A
 
D
L
 
K
G
 
V
V
 
Y
P
 
G
A
 
I
T
 
T
L
 
I
S
 
S
G
 
G
S
|
S
G
 
G
P
 
P
S
 
S
V
 
I
L
 
I
A
 
A
L
 
F

Sites not aligning to the query:

1fwkA Crystal structure of homoserine kinase complexed with adp (see paper)
28% identity, 32% coverage: 250:387/426 of query aligns to 134:264/296 of 1fwkA

query
sites
1fwkA
H
 
H
P
 
A
D
 
D
N
 
N
V
 
V
A
 
A
A
 
P
A
 
A
L
 
I
S
 
F
G
 
G
G
 
G
F
 
F
T
 
T
V
 
M
A
 
V
W
 
-
Q
 
T
D
 
N
V
 
Y
E
 
E
G
 
P
A
 
L
R
 
E
C
 
V
L
 
L
R
 
H
V
 
I
D
 
P
P
 
I
F
 
D
A
 
F
E
 
K
L
 
L
R
 
D
P
 
I
V
 
L
V
 
I
F
 
A
V
 
I
P
 
P
T
 
N
V
 
I
R
 
S
Q
 
I
S
 
N
T
|
T
E
 
K
A
 
E
S
 
A
R
 
R
G
 
E
A
 
I
L
 
L
P
 
P
V
 
K
L
 
A
V
 
V
G
 
G
L
 
L
P
 
K
D
 
D
A
 
L
A
 
V
R
 
N
T
 
N
L
 
V
G
 
G
R
 
K
A
 
A
A
 
C
L
 
G
L
 
M
A
 
V
L
 
Y
T
 
-
M
 
-
S
 
-
A
 
-
A
 
-
E
 
-
P
 
-
A
 
-
A
 
A
A
 
L
A
 
Y
Q
 
N
R
 
K
A
 
D
R
 
K
T
 
S
L
 
L
F
 
F
-
 
G
-
 
R
S
 
Y
A
 
M
T
 
M
E
 
S
D
 
D
L
 
K
L
 
V
H
 
I
Q
 
E
P
 
P
Y
 
V
R
 
R
L
 
G
P
 
K
A
 
L
A
 
I
P
 
P
A
 
N
T
 
Y
G
 
F
E
 
K
L
 
I
V
 
K
A
 
E
R
 
E
L
 
V
R
 
K
A
 
D
L
 
K
G
 
V
V
 
Y
P
 
G
A
 
I
T
 
T
L
 
I
S
 
S
G
 
G
S
 
S
G
 
G
P
 
P
S
 
S
V
 
I
L
 
I
A
 
A
L
 
F

Sites not aligning to the query:

Query Sequence

>WP_157892222.1 NCBI__GCF_000058485.1:WP_157892222.1
MTALSGAVPGAETAAPGGAVRDNPGGAVRSVSGAGDRDLSGAGDRDARGEYRSVPVVGAG
RAGADGAARRVRVRVPATSANLGPGFDAFGLALGLYDEVDVEMTASGLTVDVVGPDEVAQ
DETHLVVRAIRATFDLLGRPQPGLALRCVNRIPHGRGLGSSAAAIVAGIVAAAALDRPDL
GPEFEAGPAANQGDPGVARPGVPADRAATATAAGPAAAAGPGPGDSAGPATSVPAAAAWM
LRLAHDIEGHPDNVAAALSGGFTVAWQDVEGARCLRVDPFAELRPVVFVPTVRQSTEASR
GALPVLVGLPDAARTLGRAALLALTMSAAEPAAAAQRARTLFSATEDLLHQPYRLPAAPA
TGELVARLRALGVPATLSGSGPSVLALAVGGEQAAAAVGAASAEFSVAPLSVDRSGAQVT
RLDPER

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory