SitesBLAST
Comparing WP_158211654.1 NCBI__GCF_002019605.1:WP_158211654.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 19 hits to proteins with known functional sites (download)
4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
38% identity, 76% coverage: 2:312/410 of query aligns to 5:304/402 of 4pg7A
Sites not aligning to the query:
Q5F8J4 NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
40% identity, 71% coverage: 20:312/410 of query aligns to 22:314/435 of Q5F8J4
- L45 (≠ C45) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
- LS 45:46 (≠ CK 45:46) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).
6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
36% identity, 78% coverage: 2:319/410 of query aligns to 5:328/431 of 6dzsA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G8), L12 (≠ Y9), G13 (= G10), N14 (≠ T11), V15 (= V12), V45 (= V42), R46 (= R43), R47 (≠ D44), R52 (= R49), I63 (≠ V63), L78 (≠ A76), M79 (≠ I77), P84 (= P82), A102 (= A100), K104 (= K102), G306 (= G299), T310 (= T303)
6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
38% identity, 67% coverage: 2:276/410 of query aligns to 4:269/331 of 6a0sA
- active site: D191 (= D198), K195 (= K202)
- binding l-homoserine: K99 (= K102), N150 (= N156), G151 (= G157), T152 (= T158), Y178 (= Y185), E180 (= E187), D186 (= D193), K195 (= K202)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ Y9), G12 (= G10), T13 (= T11), V14 (= V12), L42 (= L41), V43 (= V42), R44 (= R43), D45 (= D44), K48 (= K47), R50 (= R49), A73 (= A76), M74 (≠ I77), A97 (= A100), K99 (= K102), G177 (= G184), E180 (= E187)
Sites not aligning to the query:
2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
38% identity, 67% coverage: 2:276/410 of query aligns to 4:269/331 of 2ejwA
6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
38% identity, 67% coverage: 2:276/410 of query aligns to 4:269/332 of 6a0tB
- active site: D191 (= D198), K195 (= K202)
- binding l-homoserine: N150 (= N156), G151 (= G157), T152 (= T158), Y178 (= Y185), E180 (= E187), D186 (= D193), K195 (= K202)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G8), G11 (≠ Y9), G12 (= G10), T13 (= T11), V14 (= V12), L42 (= L41), V43 (= V42), R44 (= R43), D45 (= D44), K48 (= K47), R50 (= R49), A73 (= A76), M74 (≠ I77), G75 (= G78), A97 (= A100), N98 (= N101), G177 (= G184), E180 (= E187)
Sites not aligning to the query:
7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
36% identity, 58% coverage: 81:316/410 of query aligns to 81:297/300 of 7f4cA
Sites not aligning to the query:
- binding nadp nicotinamide-adenine-dinucleotide phosphate: 6, 7, 8, 9, 10, 11, 37, 38, 39, 72, 73, 74
5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
36% identity, 58% coverage: 81:316/410 of query aligns to 81:299/302 of 5x9dA
- active site: D196 (= D198), K200 (= K202)
- binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: P82 (= P82), T100 (≠ A100), N101 (= N101), K102 (= K102), G127 (≠ A127), S131 (≠ G131), N155 (= N156), G156 (= G157), T157 (= T158), Y183 (= Y185), A184 (= A186), E185 (= E187), D191 (= D193), D196 (= D198), K200 (= K202), A281 (= A298), G282 (= G299), A286 (≠ T303)
Sites not aligning to the query:
- binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: 6, 7, 8, 9, 10, 11, 37, 38, 72, 73, 74
4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
33% identity, 53% coverage: 94:312/410 of query aligns to 108:309/319 of 4xb1A
Sites not aligning to the query:
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 8, 10, 11, 12, 13, 39, 40, 57, 91, 92, 93
4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
33% identity, 53% coverage: 94:312/410 of query aligns to 108:309/319 of 4xb2A
Sites not aligning to the query:
O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
25% identity, 78% coverage: 2:319/410 of query aligns to 558:913/916 of O81852
Sites not aligning to the query:
- 441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- 443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- 522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
- 524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
P31116 Homoserine dehydrogenase; HDH; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
25% identity, 58% coverage: 1:237/410 of query aligns to 5:258/359 of P31116
- 11:18 (vs. 7:14, 75% identical) binding NADP(+)
- T93 (≠ V80) binding NADP(+)
- K117 (= K102) binding NADP(+)
- E208 (= E187) binding substrate; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
- D219 (= D198) mutation to L: Reduces kcat 150-fold.
- K223 (= K202) mutation to V: Loss of activity.
1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
25% identity, 58% coverage: 1:237/410 of query aligns to 4:257/358 of 1tveA
1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
25% identity, 58% coverage: 1:237/410 of query aligns to 4:257/358 of 1q7gA
- active site: D218 (= D198), K222 (= K202)
- binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: G13 (= G10), V14 (≠ T11), V15 (= V12), E39 (≠ Q36), N91 (≠ G79), T92 (≠ V80), S93 (≠ E81), I97 (vs. gap), P114 (≠ A100), K116 (= K102), A143 (= A127), S173 (≠ N156), K222 (= K202)
Sites not aligning to the query:
1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
25% identity, 58% coverage: 1:237/410 of query aligns to 4:257/358 of 1ebuD
- active site: D218 (= D198), K222 (= K202)
- binding 3-aminomethyl-pyridinium-adenine-dinucleotide: G11 (= G8), A12 (≠ Y9), G13 (= G10), V14 (≠ T11), V15 (= V12), E39 (≠ Q36), A40 (≠ V37), N91 (≠ G79), S93 (≠ E81), K116 (= K102)
Sites not aligning to the query:
1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
25% identity, 58% coverage: 1:237/410 of query aligns to 4:257/358 of 1ebfA
- active site: D218 (= D198), K222 (= K202)
- binding nicotinamide-adenine-dinucleotide: I10 (= I7), A12 (≠ Y9), G13 (= G10), V14 (≠ T11), V15 (= V12), E39 (≠ Q36), A40 (≠ V37), T92 (≠ V80), S93 (≠ E81), P114 (≠ A100)
O94671 Probable homoserine dehydrogenase; HDH; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
35% identity, 30% coverage: 89:210/410 of query aligns to 110:239/376 of O94671