SitesBLAST
Comparing WP_161789654.1 NCBI__GCF_000744815.1:WP_161789654.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6bfgA Crystal structure of monotopic membrane protein (s)-mandelate dehydrogenase (see paper)
42% identity, 97% coverage: 6:363/369 of query aligns to 14:371/373 of 6bfgA
- active site: Y129 (= Y121), D156 (= D149), H272 (= H262)
- binding flavin mononucleotide: Y24 (= Y16), P77 (= P69), T78 (≠ V70), G79 (= G71), Q127 (= Q119), Y129 (= Y121), T154 (≠ N147), K248 (= K238), H272 (= H262), G273 (= G263), R275 (= R265), D301 (= D293), S302 (= S294), G303 (= G295), R305 (= R297), G324 (= G316), R325 (= R317)
P20932 (S)-mandelate dehydrogenase; MDH; L(+)-mandelate dehydrogenase; EC 1.1.99.31 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
42% identity, 97% coverage: 6:363/369 of query aligns to 16:373/393 of P20932
- PTG 79:81 (≠ PVG 69:71) binding FMN
- G81 (= G71) mutation to A: 23-fold decrease in catalytic activity with mandelate as substrate and DCPIP as electron acceptor, but no change in affinity for mandelate. Shows a modestly higher reactivity with molecular oxygen.
- S108 (= S98) binding FMN
- Q129 (= Q119) binding FMN
- T156 (≠ N147) binding FMN
- K250 (= K238) binding FMN
- DSGFR 303:307 (= DSGFR 293:297) binding FMN
- GR 326:327 (= GR 316:317) binding FMN
1huvA Crystal structure of a soluble mutant of the membrane-associated (s)- mandelate dehydrogenase from pseudomonas putida at 2.15a resolution (see paper)
41% identity, 98% coverage: 3:363/369 of query aligns to 10:347/349 of 1huvA
- active site: S105 (= S98), Y128 (= Y121), T153 (≠ N147), D155 (= D149), K224 (= K238), H248 (= H262)
- binding flavin mononucleotide: Y23 (= Y16), P76 (= P69), T77 (≠ V70), G78 (= G71), S105 (= S98), Q126 (= Q119), Y128 (= Y121), T153 (≠ N147), K224 (= K238), H248 (= H262), G249 (= G263), R251 (= R265), D277 (= D293), S278 (= S294), G279 (= G295), R281 (= R297), G300 (= G316), R301 (= R317)
2a85A Crystal structure of the g81a mutant of the active chimera of (s)- mandelate dehydrogenase in complex with its substrate 2- hydroxyoctanoate (see paper)
41% identity, 98% coverage: 3:363/369 of query aligns to 10:351/353 of 2a85A
- active site: S105 (= S98), Y128 (= Y121), T153 (≠ N147), D155 (= D149), K228 (= K238), H252 (= H262)
- binding flavin mononucleotide: Y23 (= Y16), P76 (= P69), T77 (≠ V70), A78 (≠ G71), S105 (= S98), Q126 (= Q119), Y128 (= Y121), T153 (≠ N147), K228 (= K238), H252 (= H262), G253 (= G263), R255 (= R265), D281 (= D293), S282 (= S294), G283 (= G295), R285 (= R297), G304 (= G316), R305 (= R317)
- binding (2s)-2-hydroxyoctanoic acid: Y128 (= Y121), N159 (≠ R153), G160 (≠ S154), R162 (= R156), H252 (= H262), R255 (= R265)
2a7pA Crystal structure of the g81a mutant of the active chimera of (s)- mandelate dehydrogenase in complex with its substrate 3-indolelactate (see paper)
41% identity, 98% coverage: 3:363/369 of query aligns to 10:351/353 of 2a7pA
- active site: S105 (= S98), Y128 (= Y121), T153 (≠ N147), D155 (= D149), K228 (= K238), H252 (= H262)
- binding 3-(indol-3-yl) lactate: Y128 (= Y121), R162 (= R156), H252 (= H262)
- binding flavin mononucleotide: Y23 (= Y16), P76 (= P69), T77 (≠ V70), A78 (≠ G71), S105 (= S98), Q126 (= Q119), Y128 (= Y121), T153 (≠ N147), K228 (= K238), H252 (= H262), G253 (= G263), R255 (= R265), D281 (= D293), S282 (= S294), G283 (= G295), R285 (= R297), G304 (= G316), R305 (= R317)
5zbmB Structure of glycolate oxidase containing fmn from nicotiana benthamiana (see paper)
41% identity, 96% coverage: 5:358/369 of query aligns to 12:344/353 of 5zbmB
- active site: Y128 (= Y121), D156 (= D149), H248 (= H262)
- binding flavin mononucleotide: Y23 (= Y16), Y24 (≠ L17), P76 (= P69), T77 (≠ V70), W107 (≠ L100), Q126 (= Q119), Y128 (= Y121), T154 (≠ N147), K224 (= K238), H248 (= H262), G249 (= G263), R251 (= R265), D279 (= D293), G280 (≠ S294), R283 (= R297), G302 (= G316), R303 (= R317)
6gmcA 1.2 a resolution structure of human hydroxyacid oxidase 1 bound with fmn and 4-carboxy-5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole
39% identity, 98% coverage: 3:364/369 of query aligns to 13:360/360 of 6gmcA
- active site: Y132 (= Y121), D160 (= D149), H258 (= H262)
- binding 5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole-4-carboxylate: Y26 (= Y16), M82 (≠ A72), W110 (≠ L100), Y132 (= Y121), R167 (= R156), F191 (≠ L181), L203 (≠ S207), Y206 (≠ V210), H258 (= H262), R261 (= R265)
- binding flavin mononucleotide: Y26 (= Y16), Y27 (≠ L17), A79 (≠ P69), T80 (≠ V70), A81 (≠ G71), S108 (= S98), Q130 (= Q119), Y132 (= Y121), T158 (≠ N147), K234 (= K238), H258 (= H262), G259 (= G263), R261 (= R265), D289 (= D293), G290 (≠ S294), R293 (= R297), G312 (= G316), R313 (= R317)
Q9UJM8 2-Hydroxyacid oxidase 1; HAOX1; Glycolate oxidase; GO; GOX; Glyoxylate oxidase; EC 1.1.3.15; EC 1.2.3.5 from Homo sapiens (Human) (see 2 papers)
39% identity, 98% coverage: 3:364/369 of query aligns to 13:362/370 of Q9UJM8
- Y26 (= Y16) binding glyoxylate
- ATA 79:81 (≠ PVG 69:71) binding FMN
- S108 (= S98) binding FMN
- Q130 (= Q119) binding FMN
- Y132 (= Y121) binding glyoxylate
- T158 (≠ N147) binding FMN
- R167 (= R156) binding glyoxylate
- K236 (= K238) binding FMN
- S258 (= S260) binding FMN
- H260 (= H262) binding glyoxylate
- R263 (= R265) binding glyoxylate
- DGGVR 291:295 (≠ DSGFR 293:297) binding FMN
- GR 314:315 (= GR 316:317) binding FMN
5qigA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z1407672867
39% identity, 98% coverage: 3:364/369 of query aligns to 10:359/359 of 5qigA
- active site: Y129 (= Y121), D157 (= D149), H257 (= H262)
- binding flavin mononucleotide: Y23 (= Y16), Y24 (≠ L17), A76 (≠ P69), T77 (≠ V70), A78 (≠ G71), S105 (= S98), Q127 (= Q119), Y129 (= Y121), T155 (≠ N147), K233 (= K238), H257 (= H262), G258 (= G263), R260 (= R265), D288 (= D293), G289 (≠ S294), G290 (= G295), R292 (= R297), G311 (= G316), R312 (= R317)
- binding 2-cyclopropyl-1~{H}-imidazole-4-carboxamide: R223 (= R228), G248 (= G253), F320 (≠ A325), K324 (≠ A329), D328 (≠ H333)
Sites not aligning to the query:
5qifA Pandda analysis group deposition of models with modelled events (e.G. Bound ligands) -- crystal structure of hao1 in complex with z31792168
39% identity, 98% coverage: 3:364/369 of query aligns to 10:359/359 of 5qifA
- active site: Y129 (= Y121), D157 (= D149), H257 (= H262)
- binding flavin mononucleotide: Y23 (= Y16), Y24 (≠ L17), A76 (≠ P69), T77 (≠ V70), A78 (≠ G71), S105 (= S98), Q127 (= Q119), Y129 (= Y121), K233 (= K238), H257 (= H262), G258 (= G263), R260 (= R265), D288 (= D293), G289 (≠ S294), R292 (= R297), G311 (= G316), R312 (= R317)
- binding 2-cyclohexyl-~{N}-pyridin-3-yl-ethanamide: R223 (= R228), R224 (≠ E229), S227 (≠ E232)
2rdwA Crystal structure of human glycolate oxidase in complex with sulfate (see paper)
39% identity, 98% coverage: 3:364/369 of query aligns to 10:359/359 of 2rdwA
- active site: S105 (= S98), Y129 (= Y121), T155 (≠ N147), D157 (= D149), K233 (= K238), H257 (= H262)
- binding flavin mononucleotide: Y23 (= Y16), Y24 (≠ L17), A76 (≠ P69), T77 (≠ V70), A78 (≠ G71), Q127 (= Q119), Y129 (= Y121), T155 (≠ N147), K233 (= K238), H257 (= H262), G258 (= G263), R260 (= R265), D288 (= D293), G289 (≠ S294), R292 (= R297), G311 (= G316), R312 (= R317)
- binding sulfate ion: Y23 (= Y16), W107 (≠ L100), R164 (= R156), H257 (= H262), R260 (= R265)
2rduA Crystal structure of human glycolate oxidase in complex with glyoxylate (see paper)
39% identity, 98% coverage: 3:364/369 of query aligns to 11:360/360 of 2rduA