SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_161793854.1 NCBI__GCF_000877395.1:WP_161793854.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
38% identity, 47% coverage: 244:475/494 of query aligns to 4:236/247 of 7tzpG

query
sites
7tzpG

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binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G255), R18 (= R258), G19 (= G259), I20 (= I260), D39 (≠ A279), R40 (= R280), C63 (= C301), I65 (≠ V303), N91 (= N329), G93 (= G331), I94 (= I333), V114 (= V353), Y155 (= Y395), K159 (= K399), I188 (≠ V428), T190 (= T430), T193 (≠ Q433)

3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
33% identity, 46% coverage: 249:475/494 of query aligns to 2:228/239 of 3sj7A

query
sites
3sj7A

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active site: G12 (= G259), S138 (= S382), Q148 (≠ W392), Y151 (= Y395), K155 (= K399)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G255), S10 (= S257), R11 (= R258), I13 (= I260), N31 (≠ L277), Y32 (≠ A278), A33 (= A279), G34 (≠ R280), S35 (= S281), A58 (≠ C301), N59 (≠ D302), V60 (= V303), N86 (= N329), A87 (= A330), T109 (≠ V353), S138 (= S382), Y151 (= Y395), K155 (= K399), P181 (= P425), G182 (= G426)

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
32% identity, 46% coverage: 249:475/494 of query aligns to 5:235/246 of 3osuA

query
sites
3osuA

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active site: G15 (= G259), S141 (= S382), Q151 (≠ W392), Y154 (= Y395), K158 (= K399)
binding magnesium ion: N89 (= N329), K158 (= K399)

5t2uA Short chain dehydrogenase/reductase family protein (see paper)
39% identity, 50% coverage: 244:489/494 of query aligns to 2:241/241 of 5t2uA

query
sites
5t2uA

M

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active site: G17 (= G259), T135 (≠ S382), T145 (≠ W392), Y148 (= Y395), K152 (= K399)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G255), G17 (= G259), R38 (= R280), D39 (≠ S281), R42 (≠ A284), D60 (= D302), L61 (≠ V303), N83 (= N329), A84 (= A330), Y87 (≠ D334), I133 (= I380), T135 (≠ S382), Y148 (= Y395), K152 (= K399), P178 (= P425), P180 (≠ T427), T181 (≠ V428), T183 (= T430), T185 (≠ M432), T186 (≠ Q433)

4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
32% identity, 48% coverage: 244:480/494 of query aligns to 1:241/247 of 4jroC

query
sites
4jroC

M

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M

Q

T

R

D

S

K

I

L

R

D

D

E

S

K

G

T

I

K

N

E

P

A

-

M

V

L

S

A

Q

Q

L

I

A

P

W

L

E

G

R

A

H

Y

I

G

P

T

A

T

E

E

W

D

V

I

A

A

R

N

A

A

I

V

A

L

W

F

L

L

T

A

T

S

D

D

A

A

A

S

R

K

A

Y

F

I

D

T

G

G

A

Q

D

T

F

L

S

S

L

V

active site: G16 (= G259), S142 (= S382), Q152 (≠ W392), Y155 (= Y395), K159 (= K399)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G255), S14 (= S257), R15 (= R258), G16 (= G259), I17 (= I260), N35 (vs. gap), Y36 (vs. gap), N37 (vs. gap), G38 (vs. gap), S39 (≠ A278), N63 (≠ D302), V64 (= V303), N90 (= N329), A91 (= A330), I93 (≠ L332), I113 (≠ V353), S142 (= S382), Y155 (= Y395), K159 (= K399), P185 (= P425), I188 (≠ V428), T190 (= T430)

P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 47% coverage: 244:475/494 of query aligns to 1:232/244 of P0AEK2

query
sites
P0AEK2

M

M

D

N

L

F

E

E

G

G

K

K

T

I

V

A

I

L

V

V

T

T

G
|
G

A
|
A

S
|
S

R
|
R

G

G

I

I

G

G

E

R

A

A

S

I

A

A

R

E

H

T

L

L

A

A

S

A

L

R

G

G

A

A

N

K

V

V

V

I

L

G

A

T

A

A

R
x
T

S

S

G

E

D

N

A

G

V

A

T

Q

K

A

I

I

A

S

V

D

E

Y

I

L

G

G

P

A

N

N

A

G

I

K

G

G

V

L

A

M

C

L

D
x
N

V
|
V

S

T

D

D

W

P

D

A

Q

S

A

I

A

E

A

S

T

V

V

L

A

E

Q

K

A

I

V

R

D

A

T

E

F

F

G

G

A

E

V

V

D

D

L

I

L

L

V

V

N

N

N
|
N

A

A

G

G

L

I

I

T

-

R

-

D

D

N

P

L

I

L

A

M

R

R

I

M

E

K

D

D

A

E

D

E

P

-

G

-

A

-

W

W

G

N

R

D

V

I

D

E

V

T

N

N

V

L

K

S

G

S

A

V

F

F

H

R

M

L

L

S

R

K

A

A

V

V

V

M

P

R

P

A

M

M

I

M

A

K

G

K

G

R

S

H

G

G

L

R

I

I

V

I

N

T

I

I

S

G

S

S

G

V

A

V

A

G

T

T

S

M

A

G

L

N

E

G

G

G

W

Q

S

A

H

N

Y
|
Y

C
x
A

A
|
A

T
x
A

K
|
K

A

A

A

G

L

L

L

I

S

G

L

F

T

S

R

K

C

S

A

L

H

A

K

R

E

E

L

V

A

A

P

S

K

R

G

G

V

I

N

T

V

V

I

N

G

V

L

V

S

A

P

P

G

G

T

F

V
x
I

A

E

T

T

D

D

M

M

Q

T

R

R

S

A

I

L

R

S

D

D

-

D

-

Q

-

R

S

A

G

G

I

I

N

-

P

-

V

L

S

A

Q

Q

L

V

A

P

W

A

E

G

R

R

H

L

I

G

P

G

A

A

E

Q

W

E

V

I

A

A

R

N

A

A

I

V

A

A

W

F

L

L

T

A

T

S

D

D

A

E

A

A

R

A

A

Y

F

I

D

T

G

G

GASR 12:15 (= GASR 255:258) binding NADP(+)
T37 (≠ R280) binding NADP(+)
NV 59:60 (≠ DV 302:303) binding NADP(+)
N86 (= N329) binding NADP(+)
Y151 (= Y395) mutation to F: Defect in the affinity for NADPH.
YAAAK 151:155 (≠ YCATK 395:399) binding NADP(+)
A154 (≠ T398) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
K155 (= K399) mutation to A: Defect in the affinity for NADPH.
I184 (≠ V428) binding NADP(+)

Sites not aligning to the query:

233 E→K: Decreases in the thermolability of the reductase; when associated with T-154.

3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
33% identity, 47% coverage: 244:475/494 of query aligns to 4:235/247 of 3op4A

query
sites
3op4A

M

M

D

N

L

L

E

E

G

G

K

K

T

V

V

A

I

L

V

V

T

T

G
|
G

A

A

S
|
S

R
|
R

G

G

I
|
I

G

G

E

K

A

A

S

I

A

A

R

E

H

L

L

L

A

A

S

E

L

R

G

G

A

A

N

K

V

V

V

I

L

G

A

T

A

A

R
x
T

S

S

G

E

D

S

A

G

V

A

T

Q

K

A

I

I

A

S

V

D

E

Y

I

L

G

G

P

D

N

N

A

G

I

K

G

G

V

M

A

A

C

L

D
x
N

V
|
V

S

T

D

N

W

P

D

E

Q

S

A

I

A

E

A

A

T

V

V

L

A

K

Q

A

A

I

V

T

D

D

T

E

F

F

G

G

A

G

V

V

D

D

L

I

L

L

V

V

N

N

N
|
N

A
|
A

G

G

L
x
I

I

T

-

R

-

D

D

N

P

L

I

L

A

M

R

R

I

M

E

K

D

E

A

E

D

E

P

-

G

-

A

-

W

W

G

S

R

D

V

I

V

M

D

E

V

T

N

N

V

L

K

T

G

S

A

I

F

F

H

R

M

L

L

S

R

K

A

A

V

V

V

L

P

R

P

G

M

M

I

M

A

K

G

K

G

R

S

Q

G

G

L

R

I

I

V

I

N

N

I
x
V

S

G

S
|
S

G

V

A

V

A

G

T

T

S

M

A

G

L

N

E

A

G

G

W

Q

S

A

H

N

Y
|
Y

C

A

A

A

T

A

K
|
K

A

A

A

G

L

V

L

I

S

G

L

F

T

T

R

K

C

S

A

M

H

A

K

R

E

E

L

V

A

A

P

S

K

R

G

G

V

V

N

T

V

V

I

N

G

T

L

V

S

A

P
|
P

G
|
G

T

F

V
x
I

A

E

T
|
T

D

D

M
|
M

Q

T

R

K

S

A

I

L

R

N

D

D

S

E

G

-

I

-

N

-

P

-

V

-

S

Q

Q

R

L

T

A

A

W

T

E

L

R

A

H

Q

I

V

P

P

A

A

-

G

-

R

-

L

-

G

-

D

-

P

E

R

W

E

V

I

A

A

R

S

A

A

I

V

A

A

W

F

L

L

T

A

T

S

D

P

A

E

A

A

R

A

A

Y

F

I

D

T

G

G

binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G255), S17 (= S257), R18 (= R258), I20 (= I260), T40 (≠ R280), N62 (≠ D302), V63 (= V303), N89 (= N329), A90 (= A330), I92 (≠ L332), V139 (≠ I380), S141 (= S382), Y154 (= Y395), K158 (= K399), P184 (= P425), G185 (= G426), I187 (≠ V428), T189 (= T430), M191 (= M432)

4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
34% identity, 47% coverage: 244:475/494 of query aligns to 4:231/243 of 4i08A

query
sites
4i08A

M

M

D

N

L

L

E

E

G

G

K

K

T

V

V

A

I

L

V

V

T

T

G
|
G

A

A

S
|
S

R
|
R

G
|
G

I
|
I

G

G

E

K

A

A

S

I

A

A

R

E

H

L

L

L

A

A

S

E

L

R

G

G

A

A

N

K

V

V

V

I

L

G

A

T

A

A

R
x
T

S

S

G

E

D

S

A

G

V

A

T

Q

K

A

I

I

A

S

V

D

E

Y

I

L

G

G

P

D

N

N

A

G

I

K

G

G

V

M

A

A

C

L

D
x
N

V
|
V

S

T

D

N

W

P

D

E

Q

S

A

I

A

E

A

A

T

V

V

L

A

K

Q

A

A

I

V

T

D

D

T

E

F

F

G

G

A

G

V

V

D

D

L

I

L

L

V

V

N

N

N
|
N

A
|
A

G

G

L

I

I

T

-

R

-

D

D

N

P

L

I

L

A

M

R

R

I

M

E

K

D

E

A

E

D

E

P

-

G

-

A

-

W

W

G

S

R

D

V

I

V

M

D

E

V

T

N
|
N

V

L

K

T

G

S

A

I

F

F

H

R

M

L

L

S

R

K

A

A

V

V

V

L

P

R

P

G

M

M

I

M

A

K

G

K

G

R

S

Q

G

G

L

R

I

I

V

I

N

N

I

V

S
x
G

S
|
S

G

V

A

V

A

G

T

T

S

M

A

G

L

N

E

A

G

G

W
x
Q

S

A

H

N

Y
|
Y

C

A

A

A

T

A

K
|
K

A

A

A

G

L

V

L

I

S

G

L

F

T

T

R

K

C

S

A

M

H

A

K

R

E

E

L

V

A

A

P

S

K

R

G

G

V

V

N

T

V

V

I

N

G

T

L

V

S

A

P
|
P

G
|
G

T

F

V

I

A

E

T
|
T

D

D

M

M

Q

N

R

D

S

E

I

Q

R

R

D

T

S

A

G

T

I

L

N

A

-

Q

-

V

P

P

V

A

S

G

Q

R

L

L

A

G

W

D

E

P

R

R

H

E

I

I

P

-

A

-

E

-

W

-

V

-

A

A

R

S

A

A

I

V

A

A

W

F

L

L

T

A

T

S

D

P

A

E

A

A

R

A

A

Y

F

I

D

T

G

G

active site: G19 (= G259), N113 (= N354), S141 (= S382), Q151 (≠ W392), Y154 (= Y395), K158 (= K399)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G255), S17 (= S257), R18 (= R258), I20 (= I260), T40 (≠ R280), N62 (≠ D302), V63 (= V303), N89 (= N329), A90 (= A330), G140 (≠ S381), S141 (= S382), Y154 (= Y395), K158 (= K399), P184 (= P425), G185 (= G426), T189 (= T430)

1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
33% identity, 47% coverage: 245:475/494 of query aligns to 1:231/243 of 1q7bA

query
sites
1q7bA

D

N

L

F

E

E

G

G

K

K

T

I

V

A

I

L

V

V

T

T

G
|
G

A

A

S
|
S

R
|
R

G
|
G

I

I

G

G

E

R

A

A

S

I

A

A

R

E

H

T

L

L

A

A

S

A

L

R

G

G

A

A

N

K

V

V

V

I

L

G

A

T

A

A

R
x
T

S

S

G

E

D

N

A

G

V

A

T

Q

K

A

I

I

A

S

V

D

E

Y

I

L

G

G

P

A

N

N

A

G

I

K

G

G

V

L

A

M

C

L

D
x
N

V
|
V

S

T

D

D

W

P

D

A

Q

S

A

I

A

E

A

S

T

V

V

L

A

E

Q

K

A

I

V

R

D

A

T

E

F

F

G

G

A

E

V

V

D

D

L

I

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

L
x
I

I

T

-

R

-

D

D

N

P

L

I

L

A

M

R

R

I

M

E

K

D

D

A

E

D
x
E

P

-

G

-

A

-

W

W

G

N

R

D

V

I

D

E

V

T

N

N

V

L

K

S

G

S

A

V

F

F

H

R

M

L

L

S

R

K

A

A

V

V

V

M

P

R

P

A

M

M

I

M

A

K

G

K

G

R

S

H

G

G

L

R

I

I

V

I

N

T

I

I

S

G

S
|
S

G

V

A

V

A

G

T

T

S

M

A

G

L

N

E

G

G

G

W
x
Q

S

A

H

N

Y
|
Y

C

A

A

A

T

A

K
|
K

A

A

A

G

L

L

L

I

S

G

L

F

T

S

R

K

C

S

A

L

H

A

K

R

E

E

L

V

A

A

P

S

K

R

G

G

V

I

N

T

V

V

I

N

G

V

L

V

S

A

P
|
P

G
|
G

T

F

V
x
I

A

E

T

T

D

D

M

M

Q

T

R

R

S

A

I

L

R

S

D

D

-

D

-

Q

-

R

S

A

G

G

I

I

N

-

P

-

V

L

S

A

Q

Q

L

V

A

P

W

A

E

G

R

R

H

L

I

G

P

G

A

A

E

Q

W

E

V

I

A

A

R

N

A

A

I

V

A

A

W

F

L

L

T

A

T

S

D

D

A

E

A

A

R

A

A

Y

F

I

D

T

G

G

active site: G15 (= G259), E101 (≠ D343), S137 (= S382), Q147 (≠ W392), Y150 (= Y395), K154 (= K399)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G255), S13 (= S257), R14 (= R258), T36 (≠ R280), N58 (≠ D302), V59 (= V303), N85 (= N329), A86 (= A330), G87 (= G331), I88 (≠ L332), S137 (= S382), Y150 (= Y395), K154 (= K399), P180 (= P425), G181 (= G426), I183 (≠ V428)

Sites not aligning to the query:

binding calcium ion: 232, 233

P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
33% identity, 47% coverage: 244:475/494 of query aligns to 1:232/244 of P0A2C9

query
sites
P0A2C9

M

M

D

S

L

F

E

E

G

G

K

K

T

I

V

A

I

L

V

V

T

T

G

G

A

A

S

S

R

R

G

G

I

I

G

G

E

R

A

A

S

I

A

A

R

E

H

T

L

L

A

V

S

A

L

R

G

G

A

A

N

K

V

V

V

I

L

G

A

T

A

A

R

T

S

S

G

E

D

N

A

G

V

A

T

K

K

N

I

I

A

S

V

D

E

Y

I

L

G

G

P

A

N

N

A

G

I

K

G

G

V

L

A

M

C

L

D

N

V

V

S

T

D

D

W

P

D

A

Q

S

A

I

A

E

A

S

T

V

V

L

A

E

Q

N

A

I

V

R

D

A

T

E

F

F

G

G

A

E

V

V

D

D

L

I

L

L

V

V

N

N

A

A

G

G

L

I

I

T

-

R

-

D

D

N

P

L

I

L

A

M

R

R

I

M

E

K

D

D

A

D

D

E

P

-

G

-

A

-

W

W

G

N

R

D

V

I

D

E

V

T

N

N

V

L

K

S

G

S

A

V

F

F

H

R

M

L

L

S

R

K

A

A

V

V

V

M

P

R

P

A

M
|
M

I

M

A

K

G

K

G

R

S

C

G

G

L

R

I

I

V

I

N

T

I

I

S

G

S

S

G

V

A

V

A

G

T

T

S

M

A

G

L

N

E

A

G

G

W

Q

S

A

H

N

Y

Y

C

A

A

A

T

A

K

K

A

A

A

G

L

L

L

I

S

G

L

F

T

S

R

K

C

S

A

L

H

A

K

R

E

E

L

V

A

A

P

S

K

R

G

G

V

I

N

T

V

V

I

N

G

V

L

V

S

A

P

P

G

G

T

F

V

I

A

E

T

T

D

D

M

M

Q

T

R

R

S

A

I

L

R

S

D

D

-

D

-

Q

-

R

S

A

G

G

I

I

N

-

P

-

V

L

S

A

Q

Q

L

V

A

P

W

A

E

G

R

R

H

L

I

G

P

G

A

A

E

Q

W

E

V

I

A

A

R

S

A

A

I

V

A

A

W

F

L

L

T
x
A

T
x
S

D

D

A

E

A

A

R

S

A

Y

F

I

D

T

G

G

M125 (= M369) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
A223 (≠ T466) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
S224 (≠ T467) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.

6d9yB Crystal structure of a short chain dehydrogenase/reductase sdr from burkholderia phymatum with partially occupied NAD
35% identity, 48% coverage: 244:480/494 of query aligns to 5:244/251 of 6d9yB

query
sites
6d9yB

M

I

D

D

L

L

E

E

G

G

K

R

T

V

V

V

I

V

V

I

T

T

G
|
G

A

G

S

A

R
|
R

G
|
G

I

I

G

G

E

Y

A

A

S

V

A

A

R

Q

H

R

L

A

A

L

S

R

L

S

G

G

A

A

N

A

V

V

V

A

L

L

-

W

-
x
D

-
x
L

-

D

A

A

A

E

R

R

S

L

G

A

D

R

A

S

V

E

T

R

K

E

I

L

A

A

V

-

E

E

I

L

G

G

P

-

N

K

A

V

I

C

G

A

V

V

A

T

C
x
V

D
|
D

V
x
Q

S

T

D

K

W

E

D

A

Q

Q

A

I

A

E

A

A

T

A

V

V

A

G

Q

K

A

T

V

L

D

A

T

A

F

F

G

G

A

A

V

I

D

D

L

V

L

L

V

I

N

N

N

C

A
|
A

G

G

L

I

I

T

D

G

P

G

I

N

A

G

R

T

I

T

E

W

D

E

A

L

D

E

P

P

G

D

A

V

W

W

G

R

R

Q

V

V

V

I

D

D

V

V

N

N

V

L

K

I

G

G

A

P

F

Y

H

L

M

V

L

C

R

R

A

A

V

V

P

P

P

Q

M

M

I

L

A

K

G

Q

G

G

S

Y

G

G

L

R

I

I

V

V

N

N

I

I

S

A

S
|
S

G

V

A

A

A

G

T

K

S

E

A

G

L

N

E

P

G

N

W

A

S

S

H

H

Y
|
Y

C

S

A

A

T

S

K
|
K

A

A

A

G

L

L

L

I

S

G

L

L

T

T

R

K

C

S

A

L

H

G

K

K

E

E

L

L

A

A

P

T

K

K

G

N

V

I

N

L

V

V

I

N

G

A

L

V

S

T

P
|
P

G
x
A

T
x
A

V
x
A

A

K

T
|
T

D

E

M

I

Q

F

R

D

S

S

I

M

R

K

D

Q

S

E

G

H

I

I

N

D

P

Y

-

M

V

L

S

S

Q

K

L

I

A

P

W

M

E

N

R

R

H

F

I

L

P

L

A

P

E

D

W

E

V

A

A

A

R

S

A

L

I

I

A

L

W

W

L

L

T

G

T

S

D

E

A

D

A

C

R

A

A

F

F

S

D

T

G

G

A

S

D

V

F

F

S

D

L

L

active site: G20 (= G259), S145 (= S382), Y158 (= Y395)
binding nicotinamide-adenine-dinucleotide: G16 (= G255), R19 (= R258), G20 (= G259), D40 (vs. gap), L41 (vs. gap), V64 (≠ C301), D65 (= D302), Q66 (≠ V303), A93 (= A330), S145 (= S382), Y158 (= Y395), K162 (= K399), P188 (= P425), A189 (≠ G426), A190 (≠ T427), A191 (≠ V428), T193 (= T430)

1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
33% identity, 47% coverage: 245:475/494 of query aligns to 1:231/243 of 1q7cA

query
sites
1q7cA

D

N

L

F

E

E

G

G

K

K

T

I

V

A

I

L

V

V

T

T

G
|
G

A

A

S
|
S

R
|
R

G
|
G

I

I

G

G

E

R

A

A

S

I

A

A

R

E

H

T

L

L

A

A

S

A

L

R

G

G

A

A

N

K

V

V

V

I

L

G

A

T

A
|
A

R
x
T

S

S

G

E

D

N

A

G

V

A

T

Q

K

A

I

I

A

S

V

D

E

Y

I

L

G

G

P

A

N

N

A

G

I

K

G

G

V

L

A

M

C
x
L

D
x
N

V
|
V

S

T

D

D

W

P

D

A

Q

S

A

I

A

E

A

S

T

V

V

L

A

E

Q

K

A

I

V

R

D

A

T

E

F

F

G

G

A

E

V

V

D

D

L

I

L

L

V

V

N

N

A

A

G
|
G

L
x
I

I

T

-

R

-

D

D

N

P

L

I

L

A

M

R

R

I

M

E

K

D

D

A

E

D

E

P

-

G

-

A

-

W

W

G

N

R

D

V

I

D

E

V

T

N

N

V

L

K

S

G

S

A

V

F

F

H

R

M

L

L

S

R

K

A

A

V

V

V

M

P

R

P

A

M

M

I

M

A

K

G

K

G

R

S

H

G

G

L

R

I

I

V

I

N

T

I

I

S

G

S
|
S

G

V

A

V

A

G

T

T

S

M

A

G

L

N

E

G

G

G

W
x
Q

S

A

H

N

Y
x
F

C

A

A

A

T

A

K
|
K

A

A

A

G

L

L

L

I

S

G

L

F

T

S

R

K

C

S

A

L

H

A

K

R

E

E

L

V

A

A

P

S

K

R

G

G

V

I

N

T

V

V

I

N

G

V

L

V

S

A

P

P

G

G

T

F

V

I

A

E

T

T

D

D

M

M

Q

T

R

R

S

A

I

L

R

S

D

D

-

D

-

Q

-

R

S

A

G

G

I

I

N

-

P

-

V

L

S

A

Q

Q

L

V

A

P

W

A

E

G

R

R

H

L

I

G

P

G

A

A

E

Q

W

E

V

I

A

A

R

N

A

A

I

V

A

A

W

F

L

L

T

A

T

S

D

D

A

E

A

A

R

A

A

Y

F

I

D

T

G

G

active site: G15 (= G259), S137 (= S382), Q147 (≠ W392), F150 (≠ Y395), K154 (= K399)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G255), S13 (= S257), R14 (= R258), A35 (= A279), T36 (≠ R280), L57 (≠ C301), N58 (≠ D302), V59 (= V303), G87 (= G331), I88 (≠ L332)

3pk0B Crystal structure of short-chain dehydrogenase/reductase sdr from mycobacterium smegmatis (see paper)
37% identity, 48% coverage: 241:475/494 of query aligns to 3:242/262 of 3pk0B

query
sites
3pk0B

G

G

K

S

T

M

M

F

D

D

L

L

E

Q

G

G

K

R

T

S

V

V

I

V

V

V

T

T

G

G

A

G

S

T

R

K

G
|
G

I

I

G

G

E

R

A

G

S

I

A

A

R

T

H

V

L

F

A

A

S

R

L

A

G

G

A

A

N

N

V

V

V

A

L

V

A

A

A

G

R

R

S

S

G

T

-

A

-

D

-

I

D

D

A

A

V

C

T

V

K

A

I

D

A

L

V
x
D

E

Q

I

L

G
|
G

P

S

-
x
G

N

K

A

V

I

I

G

G

V

V

A

Q

C

T

D

D

V

V

S

S

D

D

W

R

D

A

Q

Q

A

C

A

D

A

A

T

L

V

A

A

G

Q

R

A

A

V

V

D

E

T

E

F

F

G

G

A

G

V

I

D

D

L

V

V

C

N

A

N

N

A

A

G

G

L

V

I

F

D

-

P

P

I

D

A

A

R

P

I

L

E

A

D

T

A

M

D

T

P

P

G

E

A

Q

W

L

G

N

R

G

V

I

V

F

D

A

V

V

N

N

V

V

K

N

G

G

A

T

F

F

H

Y

M

A

L

V

R

Q

A

A

V

C

V

L

P

D

P

A

M

L

I

I

A

A

G

S

G

G

S

S

G

G

L

R

I

V

V

V

N

L

I

T

S

S

S
|
S

-

I

G

T

A

G

A

P

T

I

S

T

A

G

L

Y

E

P

G

G

W
|
W

S

S

H

H

Y
|
Y

C

G

A

A

T

T

K
|
K

A

A

L

Q

L

L

S

G

L

F

T

M

R

R

C

T

A

A

H

A

K

I

E

E

L

L

A

A

P

P

K

H

G

K

V

I

N

T

V

V

I

N

G

A

L

I

S

M

P

P

G

G

T

N

V

I

A

-

T

-

D

-

M

M

Q

T

R

E

S

G

I

L

R

L

D

E

S

N

G

G

I

E

N

E

P

Y

V

I

S

A

Q

S

L

M

A

A

W

-

E

-

R

R

H

S

I

I

P

P

A

A

-

G

-

A

-

L

-

G

-

T

-

P

E

E

W

D

V

I

A

G

R

H

A

L

I

A

A

A

W

F

L

L

T

A

T

T

D

K

A

E

A

A

R

G

A

Y

F

I

D

T

G

G

active site: G21 (= G259), S147 (= S382), W158 (= W392), Y161 (= Y395), K165 (= K399)
binding calcium ion: D55 (≠ V290), G58 (= G293), G60 (vs. gap)

7krmC Putative fabg bound to nadh from acinetobacter baumannii
35% identity, 47% coverage: 244:475/494 of query aligns to 1:232/244 of 7krmC

query
sites
7krmC

M

M

D

L

L

L

E

Q

G

G

K

K

T

V

V

A

I

L

V

I

T

T

G
|
G

A

A

S

A

-
x
S

-

E

R

R

G
|
G

I
|
I

G

G

E

R

A

A

S

T

A

A

R

E

H

I

L

F

A

A

S

Q

L

Q

G

G

A

A

N

K

V

V

V

I

L

I

A

V

A
x
D

R
x
L

S

D

G

L

D

A

A

Q

V

S

T

Q

K

N

I

A

A

A

V

K

E

A

I

L

G

G

P

E

N

G

A

H

I

M

G

G

V

L

A

A

C
x
A

D
x
N

V
|
V

S

A

D

N

W

E

D

E

Q

Q

A

V

A

K

A

A

T

A

V

V

A

E

Q

Q

A

A

V

L

D

Q

T

H

F

Y

G

G

A

K

V

I

D

D

L

I

L

L

V

I

N

N

N
|
N

A

A

G

G

L

I

I

T

D

Q

P

P

I

I

A

K

R

T

I

L

E

-

D

D

A

I

D

Q

P

R

G

S

A

D

W

Y

G

D

R

R

V

V

V

L

D

D

V
|
V

N

S

V

L

K

R

G

G

A

T

F

L

H

I

M

M

L

S

R

Q

A

A

V

V

V

I

P

P

P

S

M

M

I

K

A

A

G

N

G

G

S

G

G

G

L

S

I

I

V

V

N

C

I

L

S

S

S
|
S

G

V

A

S

A

A

T

Q

-

R

-

G

S

G

A

G

L

I

E

F

G

G

W

G

S

P

H

H

Y
|
Y

C

S

A

A

T

A

K
|
K

A

A

A

G

L

V

L

L

S

G

L

L

T

A

R

K

C

A

A

M

H

A

K

R

E

E

L

F

A

G

P

G

K

D

G

Q

V

I

N

R

V

V

I

N

G

S

L

L

S

T

P

P

G

G

T

L

V
x
I

A

Q

T
|
T

D

D

M

M

Q

N

R

D

S

D

I

R

R

R

D

H

-

D

-

I

-

L

S

A

G

G

I

I

N

-

P

P

V

L

S

G

Q

R

L

L

A

G

W

-

E

-

R

-

H

-

I

-

P

K

A

A

E

Q

W

D

V

V

A

A

R

N

A

A

I

A

A

L

W

F

L

L

T

A

T

S

D

D

A

L

A

S

R

A

A

Y

F

L

D

T

G

G

active site: G18 (= G259), S140 (= S382), Y155 (= Y395)
binding nicotinamide-adenine-dinucleotide: G12 (= G255), S15 (vs. gap), G18 (= G259), I19 (= I260), D38 (≠ A279), L39 (≠ R280), A60 (≠ C301), N61 (≠ D302), V62 (= V303), N88 (= N329), V111 (= V353), S140 (= S382), Y155 (= Y395), K159 (= K399), I188 (≠ V428), T190 (= T430)

7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
34% identity, 46% coverage: 246:470/494 of query aligns to 2:226/243 of 7emgB

query
sites
7emgB

L

F

E

E

G

G

K

K

T

I

V

V

I

L

V

V

T

T

G
|
G

A

A

S
|
S

R
|
R

G

G

I

I

G

G

E

R

A

A

S

I

A

A

R

E

H

T

L

F

A

V

S

A

L

R

G

G

A

A

N

K

V

V

V

I

L

G

A

T

A

A

R
x
T

S

S

G

E

D

S

A

G

V

A

T

E

K

A

I

I

A

S

V

G

E

Y

I

L

G

G

P

A

N

N

A

G

I

K

G

G

V

F

A

M

C

L

D
x
N

V
|
V

S

K

D

D

W

A

D

Q

Q

S

A

I

A

D

A

S

T

V

V

L

A

A

Q

S

A

I

V

R

D

A

T

E

F

F

G

G

A

E

V

I

D

D

L

I

L

L

V

V

N

N

A

A

G

G

L
x
I

I

T

-

R

-

D

D

N

P

L

I

L

A

M

R

R

I

M

E

K

D

D

A

D

D

E

P

-

G

-

A

-

W

W

G

E

R

D

V

I

V

L

D

D

V

T

N

N

V

L

K

T

G

S

A

V

F

F

H

R

M

L

L

S

R

K

A

A

V

V

V

M

P

C

P

A

M

M

I

M

A

K

G

K

G

R

S

F

G

G

L

R

I

I

V

I

N

T

I

I

S

G

S

S

G

V

A

V

A

G

T

T

S

M

A

G

L

N

E

A

G

G

W

Q

S

A

H

N

Y

Y

C

A

A

A

T

A

K

K

A

A

A

G

L

L

L

I

S

G

L

F

T

S

R

K

C

S

A

L

H

A

K

R

E

E

L

V

A

A

P

S

K

R

G

G

V

I

N

T

V

V

I

N

G

V

L

V

S

A

P

P

G

G

T

Y

V

I

A

E

T

T

D

D

M

M

Q

T

R

R

S

A

I

L

R

T

D

D

-

D

-

Q

-

R

S

A

G

G

I

I

N

-

P

-

V

L

S

S

Q

S

L

V

A

P

W

A

E

N

R

R

H

P

I

G

P

D

A

A

E

K

W

E

V

I

A

A

R

S

A

A

I

V

A

A

W

F

L

L

T

A

T

S

D

D

A

E

A

A

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G255), S13 (= S257), R14 (= R258), T36 (≠ R280), N58 (≠ D302), V59 (= V303), I88 (≠ L332)

4nbuB Crystal structure of fabg from bacillus sp (see paper)
34% identity, 47% coverage: 246:475/494 of query aligns to 5:233/244 of 4nbuB

query
sites
4nbuB

L

L

E

Q

G

D

K

K

T

V

V

A

I

I

V

I

T

T

G
|
G

A

A

S

A

R
x
N

G
|
G

I
|
I

G

G

E

L

A

E

S

A

A

A

R

R

H

V

L

F

A

M

S

K

L

E

G

G

A

A

N

K

V

V

L

I

A

A

A
x
D

R
x
F

S

N

G

-

D

E

A

A

V

A

T

G

K

K

I

E

A

A

V

V

E

E

I

A

G

N

P

P

N

G

A

V

I

V

G

F

V

I

A

R

C
x
V

D
|
D

V
|
V

S

S

D

D

W

R

D

E

Q

S

A

V

A

H

A

R

T

L

V

V

A

E

Q

N

A

V

V

A

D

E

T

R

F

F

G

G

A

K

V

I

D

D

L

I

L

L

V

I

N

N

N
|
N

A
|
A

G
|
G

L
x
I

I

T

D

R

P
x
D

I

-

A

S

R

M

I

L

E

S

D
x
K

A

M

D

T

P

V

G

D

A

Q

W

F

G

Q

R

Q

V

V

V

I

D

N

V

V

N
|
N

V

L

K

T

G

G

A

V

F

F

H

H

M

C

L

T

R

Q

A

A

V

V

V

L

P

P

P

Y

M

M

I

A

A

E

G

Q

G

G

S

K

G

G

L

K

I

I

V

I

N

N

I
x
T

S

S

S
|
S

G

V

A

T

A

G

T

T

S

Y

A

G

L
x
N

E
x
V

G

G

W
x
Q

S

T

H

N

Y
|
Y

C

A

A

A

T

A

K
|
K

A

A

A

G

L

V

L

I

S

G

L

M

T

T

R

K

C

T

A

W

H

A

K

K

E

E

L

L

A

A

P

R

K

K

G

G

V

I

N

N

V

V

I

N

G

A

L

V

S

A

P
|
P

G

G

T
x
F

V
x
T

A

E

T
|
T

D

A

M
|
M

Q

V

R

A

S

E

I

V

R

P

D

E

S

K

G

V

I

I

N

E

P
x
K

V

M

-

K

S

A

Q

Q

L

V

A

P

W

M

E

G

R

R

H

L

I

G

P

K

A

P

E

E

W

D

V

I

A

A

R

N

A

A

I

Y

A

L

W

F

L

L

T

A

T

S

D

H

A

E

A

S

R

D

A

Y

F

V

D

N

G

G

active site: G18 (= G259), N111 (= N354), S139 (= S382), Q149 (≠ W392), Y152 (= Y395), K156 (= K399)
binding acetoacetyl-coenzyme a: D93 (≠ P335), K98 (≠ D341), S139 (= S382), N146 (≠ L389), V147 (≠ E390), Q149 (≠ W392), Y152 (= Y395), F184 (≠ T427), M189 (= M432), K200 (≠ P443)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G255), N17 (≠ R258), G18 (= G259), I19 (= I260), D38 (≠ A279), F39 (≠ R280), V59 (≠ C301), D60 (= D302), V61 (= V303), N87 (= N329), A88 (= A330), G89 (= G331), I90 (≠ L332), T137 (≠ I380), S139 (= S382), Y152 (= Y395), K156 (= K399), P182 (= P425), F184 (≠ T427), T185 (≠ V428), T187 (= T430), M189 (= M432)

P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see paper)
37% identity, 46% coverage: 246:473/494 of query aligns to 4:232/247 of P73574

query
sites
P73574

L

L

E

T

G

A

K

Q

T

V

V

A

I

L

V

V

T

T

G

G

A
|
A

S

S

R

R

G

G

I

I

G

G

E

K

A

A

S

T

A

A

R

L

H

A

L

L

A

A

S

A

L

T

G

G

A

M

N

K

V

V

L

V

-

N

A

Y

A

A

R

Q

S

S

G

S

D

T

A

A

V

A

T

D

K

A

I

V

A

V

V

A

E

E

I

I

-

I

-

A

-

N

G

G

P

G

N

E

A

A

I

I

G

A

V

V

A

Q

C

A

D

N

V

V

S

A

D

N

W

A

D

D

Q

E

A

V

A

D

A

Q

T

L

V

I

A

K

Q

T

A

T

V

L

D

D

T

K

F

F

G

S

A

R

V

I

D

D

L

V

L

L

V

V

N

N

A

A

G

G

L

I

I

T

-

R

D

D

P

T

I

L

-

L

-

R

A

M

R

K

I

L

E

E

D

D

A

-

D

-

P

-

G

-

A

-

W

W

G

Q

R

A

V

V

V

I

D

D

V

L

N

N

V

L

K

T

G

G

A

V

F

F

H

L

M

C

L

T

R

K

A

A

V

V

V

S

P

K

P

L

M

M

I

L

A

K

G

Q

G

K

S

S

G

G

L

R

I

I

V

I

N

N

I

I

S

T

S

S

G

V

A

A

A

G

T

M

S

M

A

G

L

N

E
x
P

G

G

W

Q

S

A

H

N

Y

Y

C

S

A

A

T

A

K
|
K

A

A

A

G

L

V

L

I

S

G

L

F

T

T

R

K

C

T

A

V

H

A

K

K

E

E

L

L

A

A

P

S

K

R

G

G

V

V

N

T

V

V

I

N

G

A

L

V

S

A

P

P

G

G

T
x
F

V

I

A

A

T

T

D

D

M

M

Q

T

R

E

S

N

I

L

R
x
N

D

A

S

E

G

-

I

-

N

-

P

P

V

I

S

L

Q

Q

-

F

L

I

A

P

W

L

E

A

R

R

H

Y

I

G

P

Q

A

P

E

E

W

E

V

V

A

A

R

G

A

T

I

I

A

R

W

F

L

L

T

A

T

T

D

D

A

P

A

A

R

A

A

A

F

Y

A14 (= A256) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
P151 (≠ E390) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
K160 (= K399) mutation to A: Almost no activity on acetoacetyl-CoA.
F188 (≠ T427) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
N198 (≠ R437) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.

5t5qC Crystal structure of short-chain dehydrogenase/reductase sdr:glucose/ribitol dehydrogenase from brucella melitensis
34% identity, 47% coverage: 244:475/494 of query aligns to 3:233/245 of 5t5qC

query
sites
5t5qC

M

F

D

E

L

F

E

E

G

N

K

R

T

T

V

L

I

V

V

L

T

T

G

G

A

A

S
x
N

R
x
G

G
|
G

I
|
I

G

G

E

R

A

A

S

I

A

A

R

E

H

L

L

F

A

H

S

A

L

S

G

G

A

A

N

N

V

L

V

V

L

L

A

T

A
x
D

R
x
L

S

D

G

R

D

E

A

G

V

L

T

D

K

A

I

F

A

A

V

A

E

S

I

L

G

G

-

S

P

P

N

E

A

R

I

I

G

A

-

T

V

I

A

K

C

A

D
|
D

V
x
A

S

S

D

S

W

A

D

D

Q

D

A

A

A

E

A

K

T

T

V

V

A

A

Q

L

A

A

V

M

D

E

T

R

F

F

G

G

A

G

V

I

D

D

L

F

L

L

V

V

N

P

N
x
S

A

A

G

G

L

I

I

Y

D

Q

-

A

-

K

P

P

I

F

A

A

R

E

I

M

E

S

D

D

A

A

D

D

P

-

G

-

A

-

W

W

G

H

R

R

V

T

V

I

D

S

V
x
I

N

N

V

L

K

D

G

G

A

V

F

F

H

Y

M

L

L

C

R

K

A

R

V

A

V

L

P

P

P

A

M

L

I

K

A

E

G

D

G

S

S

S

G

-

L

-

I

I

V

V

N

T

I

L

S

A

S
|
S

G

L

A

A

T

Y

S

R

A

G

L

A

E

Y

G

V

W
x
N

S

A

H

H

Y
|
Y

C

G

A

A

T

T

K
|
K

A

G

A

A

L

M

L

V

S

S

L

M

T

T

R

R

C

A

A

L

H

S

K

R

E

E

L

L

A

A

P

P

K

K

G

-

V

T

N

R

V

V

I

N

G

G

L

V

S

S

P
|
P

G

G

T

I

V
x
I

A

E

T
|
T

D

P

M
|
M

Q

T

R

S

S

E

I

L

R

L

D

K

S

T

G

R

I

M

N

D

P

E

-

T

V

M

S

T

Q

Q

L

T

A

P

W

L

E

K

R

R

H

L

I

G

P

K

A

P

E

S

W

E

V

I

A

A

R

S

A

V

I

I

A

A

W

F

L

L

T

C

T

S

D

P

A

A

R

S

A

F

F

V

D

T

G

G

active site: G18 (= G259), S140 (= S382), N150 (≠ W392), Y153 (= Y395), K157 (= K399)
binding nicotinamide-adenine-dinucleotide: N16 (≠ S257), G17 (≠ R258), G18 (= G259), I19 (= I260), D38 (≠ A279), L39 (≠ R280), D63 (= D302), A64 (≠ V303), S90 (≠ N329), I113 (≠ V353), Y153 (= Y395), K157 (= K399), P182 (= P425), I185 (≠ V428), T187 (= T430), M189 (= M432)

6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
32% identity, 47% coverage: 244:475/494 of query aligns to 1:232/244 of 6t77A

query
sites
6t77A

M

M

D

S

L

F

E

E

G

G

K

K

T

I

V

A

I

L

V

V

T

T

G
|
G

A

A

S
|
S

R
|
R

G
|
G

I

I

G

G

E

R

A

A

S

I

A

A

R

E

H

T

L

L

A

V

S

A

L

R

G

G

A

A

N

K

V

V

V

I

L

G

A

T

A

A

R
x
T

S

S

G

E

D

S

A

G

V

A

T

Q

K

A

I

I

A

S

V

D

E

Y

I

L

G

G

P

A

N

N

A

G

I

K

G

G

V

L

A

M

C
x
L

D
x
N

V
|
V

S

T

D

D

W

P

D

A

Q

S

A

I

A

E

A

S

T

V

V

L

A

E

Q

N

A

V

V

R

D

A

T

E

F

F

G

G

A

E

V

V

D

D

L

I

L

L

V

V

N

N

A
|
A

G
|
G

L
x
I

I

T

-

R

-

D

D

N

P

L

I

L

A

M

R

R

I

M

E

K

D

D

A

D

D

E

P

-

G

-

A

-

W

W

G

N

R

D

V

I

D

E

V

T

N

N

V

L

K

S

G

S

A

V

F

F

H

R

M

L

L

S

R

K

A

A

V

V

V

M

P

R

P

A

M

M

I

M

A

K

G

K

G

R

S

H

G

G

L

R

I

I

V

I

N

T

I

I

S

G

S
|
S

G

V

A

V

A

G

T

T

S

M

A

G

L

N

E

A

G

G

W

Q

S

A

H

N

Y
|
Y

C

A

A

A

T

A

K

K

A

A

A

G

L

L

L

I

S

G

L

F

T

S

R

K

C

S

A

L

H

A

K

R

E

E

L

V

A

A

P

S

K

R

G

G

V

I

N

T

V

V

I

N

G

V

L

V

S

A

P

P

G

G

T

F

V

I

A

E

T

T

D

D

M

M

Q

T

R

R

S

A

I

L

R

T

D

D

S

E

G

Q

-

R

I

A

N

G

P

T

V

L

S

A

Q

A

L

V

A

P

W

A

E

G

R

R

H

L

I

G

P

T

A

P

E

N

W

E

V

I

A

A

R

S

A

A

I

V

A

A

W

F

L

L

T

A

T

S

D

D

A

E

A

A

R

S

A

Y

F

I

D

T

G

G

active site: G16 (= G259), S138 (= S382), Y151 (= Y395)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G255), S14 (= S257), R15 (= R258), T37 (≠ R280), L58 (≠ C301), N59 (≠ D302), V60 (= V303), A87 (= A330), G88 (= G331), I89 (≠ L332)

C1DMX5 L-rhamnose 1-dehydrogenase (NAD(P)(+)); RhaDH; AvLRA1; EC 1.1.1.378 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
38% identity, 49% coverage: 244:485/494 of query aligns to 1:250/256 of C1DMX5

query
sites
C1DMX5

M

M

D

L

L

L

E

I

G

D

K

K

T

T

V

V

I

I

V

V

T

T

G
|
G

A

A

S
|
S

R
|
R

G

G

I
|
I

G

G

E

R

A

A

S

A

A

A

R

R

H

E

L

C

A

A

S

R

L

Q

G

G

A

A

N

R

V

V

L

I

A

G

A

H

R
x
S

S

G

G

S

D

D

-

E

-

G

-

R

-

A

A

G

V

A

T

L

K

S

I

L

A

A

V

E

E

E

I

I

-

A

-

F

G

G

P

G

N

T

A

A

I

I

G

A

V

V

A

G

C

A

D
|
D

V
x
A

S

A

D

D

W

L

D

D

Q

S

A

G

A

E

A

K

T

L

V

V

A

A

Q

A

A

A

V

V

D

E

T

A

F

F

G

G

A

S

V

V

D

D

L

V

L

L

V

V

N

N

N
|
N

A

A

G

G

L

-

I

I

D

C

P

P

I
x
F

A

H

R

S

I

F

E

L

D

D

A

M

D

P

P

R

G

E

A

L

W

Y

G

L

R

K

V

T

V

V

D

G

V

T

N

N

V

L

K

N

G

G

A

A

F

Y

H

F

M

T

L

V

R

Q

A

A

V

A

P

R

M

M

I

K

A

E

G

Q

G

G

-

R

S

G

G

G

L

A

I

I

V

I

N

A

I

V

S

S

S
|
S

G

I

A
x
S

A

A

T

L

S

V

A

G

L

G

E

A

G

M

W
x
Q

S

T

H

H

Y
|
Y

C

T

A

P

T

T

K
|
K

A

A

A

G

L

L

S

S

L

L

T

M

R

Q

C

S

A

C

H

A

K

I

E

A

L

L

A

G

P

P

K

Y

G

G

V

I

N

R

V

C

I

N

G

A

L

V

S

L

P

P

G

G

T
|
T

V
x
I

A

A

T

T

D

D

M
x
I

Q
x
N

R

K

-

E

-
x
D

-

L

S

S

I

D

R

L

D

E

S

K

G

R

I

E

N

R

P

M

V

T

S

S

Q

R

L

V

A

P

W

L

E

G

R

R

H

L

I

G

P

E

A

P

E

D

W

D

V

L

A

A

R

G

A

P

I

I

A

V

W

F

L

L

T

A

T

S

D

D

A

M

A

A

R

R

A

Y

F

V

D

T

G

G

A

A

D

-

F

-

S

S

L

L

K

L

T

V

D

D

E

G

G

G

G12 (= G255) binding NADP(+)
S14 (= S257) binding NADP(+)
R15 (= R258) binding NADP(+); mutation to T: Increases specificity toward NAD(+). Shows a strong decrease in catalytic efficiency with NADP(+).
I17 (= I260) binding NADP(+)
S37 (≠ R280) binding NADP(+); mutation to H: Increases specificity toward NAD(+). Shows a strong decrease in catalytic efficiency with NADP(+) and an increase in catalytic efficiency with NAD(+).
D66 (= D302) binding NADP(+)
A67 (≠ V303) binding NADP(+)
N93 (= N329) binding NADP(+)
F99 (≠ I336) mutation F->A,Y: Shows a strong decrease in catalytic efficiency with L-rhamnose, L-lyxose and L-mannose.
S146 (= S382) binding beta-L-rhamnose
S148 (≠ A384) binding beta-L-rhamnose
Q156 (≠ W392) binding beta-L-rhamnose; mutation to A: Almost loss of activity with L-rhamnose as substrate.
Y159 (= Y395) binding beta-L-rhamnose; binding NADP(+)
K163 (= K399) binding NADP(+)
T191 (= T427) binding beta-L-rhamnose; mutation to F: Retains 4% of wild-type activity with L-rhamnose as substrate.
I192 (≠ V428) binding NADP(+)
I196 (≠ M432) mutation to A: Shows a strong decrease in catalytic efficiency with L-rhamnose as substrate, but does not affect catalytic efficiency with L-lyxose and L-mannose.
N197 (≠ Q433) binding beta-L-rhamnose
D200 (vs. gap) mutation to A: Retains 16% of wild-type activity with L-rhamnose as substrate.; mutation to H: Retains 22% of wild-type activity with L-rhamnose as substrate.

Query Sequence

>WP_161793854.1 NCBI__GCF_000877395.1:WP_161793854.1
MMKTTMAACIALACAAPALAEPTEWTLDLGHAHIGWEIDHMNMANTVGRFNSFDGTFLID
EAEPANSRITFTIDASSIDSNHTGRDDHLRNPDYLNVEAHPQIDFVSTEVQMLTPTTGKL
RGDLTMLGVTAPVELDFEMVNRRTYPDFIPNYDEVEVVGFHATGEIARLDHGMDFIAFVG
SPTGFVVTLDARFDLVRCDGVPETNVPCHWGRVAELGAAAGNRTTDIGTGRRPVHPHLTI
GKTMDLEGKTVIVTGASRGIGEASARHLASLGANVVLAARSGDAVTKIAVEIGPNAIGVA
CDVSDWDQAAATVAQAVDTFGAVDLLVNNAGLIDPIARIEDADPGAWGRVVDVNVKGAFH
MLRAVVPPMIAGGSGLIVNISSGAATSALEGWSHYCATKAALLSLTRCAHKELAPKGVNV
IGLSPGTVATDMQRSIRDSGINPVSQLAWERHIPAEWVARAIAWLTTDAARAFDGADFSL
KTDEGREAVGLPTA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory