SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_169717299.1 NCBI__GCF_002252445.1:WP_169717299.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
36% identity, 95% coverage: 16:505/517 of query aligns to 4:490/501 of P04983

query
sites
P04983

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K43 (= K55) mutation to R: Loss of transport.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
29% identity, 42% coverage: 15:231/517 of query aligns to 1:215/241 of 4u00A

query
sites
4u00A

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binding adenosine-5'-diphosphate: F12 (= F26), V17 (≠ A31), S37 (≠ N51), G38 (= G52), S39 (≠ A53), G40 (= G54), K41 (= K55), S42 (= S56), T43 (= T57)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 50% coverage: 16:271/517 of query aligns to 1:259/343 of P30750

query
sites
P30750

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40:46 (vs. 51:57, 86% identical) binding ATP
E166 (= E178) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
29% identity, 42% coverage: 16:231/517 of query aligns to 1:215/240 of 4ymuJ

query
sites
4ymuJ

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binding adenosine-5'-triphosphate: F11 (= F26), V16 (≠ A31), S36 (≠ N51), G37 (= G52), S38 (≠ A53), G39 (= G54), K40 (= K55), S41 (= S56), T42 (= T57), E162 (= E178), H194 (= H210)
binding magnesium ion: S41 (= S56), E162 (= E178)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
31% identity, 50% coverage: 16:271/517 of query aligns to 2:260/344 of 6cvlD

query
sites
6cvlD

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L

V

N

P

R

A

G

G

E

Q

I

I

H

Y

A

G

L

V

L

I

G

G

E

A

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

I

C

L

V

T

N

G

L

A

L

Y

E

H

R

G

P

F

T

D

E

G

G

S

S

V

V

L

L

L

V

D

D

G

G

H

Q

A

E

I

L

A

T

P

T

A

L

S

S

V

E

A

S

E

E

A

L

Q

T

T

K

L

A

-

R

-

R

G

Q

I

I

G

G

T

M

V

I

Y

F

Q

Q

E

H

V

F

N

N

L

L

L

L

E

S

N

S

L

R

T

T

V

V

A

F

E

G

N

N

L

V

F

A

L

L

G

-

R

-

Q

-

P

P

R

L

R

E

F

L

G

D

F

N

I

T

D

P

R

K

R

D

R

E

M

V

E

K

K

R

H

R

A

V

T

T

T

E

L

L

L

L

A

S

R

L

Y

V

G

G

L

L

T

G

I

D

D

K

V

H

G

D

A

S

P

Y

L

P

S

S

A
x
N

Y

L

S
|
S

V

G

A

G

I
x
Q

R

K

Q

Q

I

R

I

V

A

A

I

I

A

A

R

R

A

A

V

L

D

A

V

S

S

N

G

P

K

K

V

V

L

L

V

L

L

C

D

D

E

Q

P

A

T

T

A

S

S

A

L

L

D

D

A

P

H

A

E

T

V

T

E

R

M

S

L

I

F

L

G

E

V

L

L

L

R

K

Q

D

L

I

Q

N

A

R

E

R

-

L

G

G

L

L

G

T

I

I

I

L

I

L

I

I

T

T

H
|
H

F

E

L

M

D

D

Q

V

V

V

Y

K

A

R

I

I

A

C

D

D

S

C

A

V

T

A

V

V

L

I

R

S

N

N

G

G

R

E

L

L

V

I

G

E

S

Q

R

D

D

T

L

V

S

S

E

E

L

V

-

F

-

S

-

H

P

P

R

K

T

T

D

P

L

L

I

A

S

Q

M

K

M

F

L

-

G

-

H

-

Q

-

L

I

Q

Q

E

S

T

T

V

L

R

H

R

L

H

D

L

I

T

P

E

E

D

D

V

Y

S

Q

D

E

N

R

T

L

T

Q

A

A

binding phosphothiophosphoric acid-adenylate ester: F12 (= F26), Q14 (≠ G28), I19 (≠ V29), S41 (≠ N51), G42 (= G52), A43 (= A53), G44 (= G54), K45 (= K55), S46 (= S56), T47 (= T57), N141 (≠ A152), S143 (= S154), Q146 (≠ I157), H200 (= H210)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
31% identity, 50% coverage: 16:271/517 of query aligns to 2:260/344 of 3tuzC

query
sites
3tuzC

L

M

L

I

E

K

A

L

R

S

S

N

I

I

T

T

K

K

S

V

F
|
F

L

H

G
x
Q

-

G

-

T

-

R

-

T

V

I

T

Q

A

A

L

L

D

N

R

N

V

V

D

S

F

L

V

H

L

V

N

P

R

A

G

G

E

Q

I

I

H

Y

A

G

L

V

L

I

G

G

E

A

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

I

C

L

V

T

N

G

L

A

L

Y

E

H

R

G

P

F

T

D

E

G

G

S

S

V

V

L

L

L

V

D

D

G

G

H

Q

A

E

I

L

A

T

P

T

A

L

S

S

V

E

A

S

E

E

A

L

Q

T

T

K

L

A

-

R

-

R

G

Q

I

I

G

G

T

M

V

I

Y

F

Q

Q

E

H

V

F

N

N

L

L

L

L

E

S

N

S

L

R

T

T

V

V

A

F

E

G

N

N

L

V

F

A

L

L

G

-

R

-

Q

-

P

P

R

L

R

E

F

L

G

D

F

N

I

T

D

P

R

K

R

D

R

E

M

V

E

K

K

R

H

R

A

V

T

T

T

E

L

L

L

L

A

S

R

L

Y

V

G

G

L

L

T

G

I

D

D

K

V

H

G

D

A

S

P

Y

L

P

S

S

A

N

Y

L

S

S

V

G

A

G

I

Q

R

K

Q

Q

I

R

I

V

A

A

I

I

A

A

R

R

A

A

V

L

D

A

V

S

S

N

G

P

K

K

V

V

L

L

V

L

L

C

D

D

E

Q

P

A

T

T

A

S

S

A

L

L

D

D

A

P

H

A

E

T

V

T

E

R

M

S

L

I

F

L

G

E

V

L

L

L

R

K

Q

D

L

I

Q

N

A

R

E

R

-

L

G

G

L

L

G

T

I

I

I

L

I

L

I

I

T

T

H

H

F

E

L

M

D

D

Q

V

V

V

Y

K

A

R

I

I

A

C

D

D

S

C

A

V

T

A

V

V

L

I

R

S

N

N

G

G

R

E

L

L

V

I

G

E

S

Q

R

D

D

T

L

V

S

S

E

E

L

V

-

F

-

S

-

H

P

P

R

K

T

T

D

P

L

L

I

A

S

Q

M

K

M

F

L

-

G

-

H

-

Q

-

L

I

Q

Q

E

S

T

T

V

L

R

H

R

L

H

D

L

I

T

P

E

E

D

D

V

Y

S

Q

D

E

N

R

T

L

T

Q

A

A

binding adenosine-5'-diphosphate: F12 (= F26), Q14 (≠ G28), G42 (= G52), G44 (= G54), K45 (= K55), S46 (= S56), T47 (= T57)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
31% identity, 50% coverage: 16:271/517 of query aligns to 2:260/344 of 3tuiC

query
sites
3tuiC

L

M

L

I

E

K

A

L

R

S

S

N

I

I

T

T

K

K

S

V

F
|
F

L

H

G

Q

-

G

-

T

-

R

-

T

V

I

T

Q

A

A

L

L

D

N

R

N

V

V

D

S

F

L

V

H

L

V

N

P

R

A

G

G

E

Q

I

I

H

Y

A

G

L

V

L

I

G

G

E

A

N

S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

I

C

L

V

T

N

G

L

A

L

Y

E

H

R

G

P

F

T

D

E

G

G

S

S

V

V

L

L

L

V

D

D

G

G

H

Q

A

E

I

L

A

T

P

T

A

L

S

S

V

E

A

S

E

E

A

L

Q

T

T

K

L

A

-

R

-

R

G

Q

I

I

G

G

T

M

V

I

Y

F

Q

Q

E

H

V

F

N

N

L

L

L

L

E

S

N

S

L

R

T

T

V

V

A

F

E

G

N

N

L

V

F

A

L

L

G

-

R

-

Q

-

P

P

R

L

R

E

F

L

G

D

F

N

I

T

D

P

R

K

R

D

R

E

M

V

E

K

K

R

H

R

A

V

T

T

T

E

L

L

L

L

A

S

R

L

Y

V

G

G

L

L

T

G

I

D

D

K

V

H

G

D

A

S

P

Y

L

P

S

S

A

N

Y

L

S

S

V

G

A

G

I

Q

R

K

Q

Q

I

R

I

V

A

A

I

I

A

A

R

R

A

A

V

L

D

A

V

S

S

N

G

P

K

K

V

V

L

L

V

L

L

C

D

D

E

Q

P

A

T

T

A

S

S

A

L

L

D

D

A

P

H

A

E

T

V

T

E

R

M

S

L

I

F

L

G

E

V

L

L

L

R

K

Q

D

L

I

Q

N

A

R

E

R

-

L

G

G

L

L

G

T

I

I

I

L

I

L

I

I

T

T

H

H

F

E

L

M

D

D

Q

V

V

V

Y

K

A

R

I

I

A

C

D

D

S

C

A

V

T

A

V

V

L

I

R

S

N

N

G

G

R

E

L

L

V

I

G

E

S

Q

R

D

D

T

L

V

S

S

E

E

L

V

-

F

-

S

-

H

P

P

R

K

T

T

D

P

L

L

I

A

S

Q

M

K

M

F

L

-

G

-

H

-

Q

-

L

I

Q

Q

E

S

T

T

V

L

R

H

R

L

H

D

L

I

T

P

E

E

D

D

V

Y

S

Q

D

E

N

R

T

L

T

Q

A

A

binding adenosine-5'-diphosphate: F12 (= F26), G42 (= G52), A43 (= A53), G44 (= G54), K45 (= K55), S46 (= S56), T47 (= T57)

8k1pB Mycobacterial efflux pump, adp+vanadate bound state
33% identity, 43% coverage: 17:236/517 of query aligns to 4:213/213 of 8k1pB

query
sites
8k1pB

L

I

E

E

A

I

R

R

S

G

I

L

T

T

K

K

S

H

F
|
F

L

G

G

S

V

V

T

R

A

A

L

L

D

D

R

G

V

L

D

D

F

L

V

T

L

V

N

R

R

E

G

G

E

E

I

V

H

H

A

G

L

F

L

L

G

G

E

P

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

T

I

L

K

R

I

I

L

L

T

L

G

G

A

L

Y

V

H

K

G

A

F

D

D

G

G

G

S

S

V

V

L

R

L

L

D

L

G

G

H

G

A

-

I

-

A

D

P

P

A

W

S

T

V

D

A

A

E

V

A

D

Q

L

T

H

L

R

G

H

I

I

G

A

T

Y

V

V

Y

P

Q

G

E

D

V

V

N

T

L

L

L

W

E

P

N

S

L

L

T

T

V

G

A

G

E

E

N

T

L

I

-

D

F

L

L

L

G

A

R

R

Q

M

P

-

R

-

R

R

F

G

G

G

F

I

I

D

D

N

R

A

R

R

R

R

M

A

E

E

K

-

H

-

A

-

T

-

T

-

L

L

L

I

A

E

R

R

Y

F

G

G

L

L

T

-

I

-

D

D

V

P

G

T

A

K

P

K

L

A

S

R

A
x
T

Y

Y

S
|
S

V

K

A

G

I

N

R

R

Q

Q

I

K

I

V

A

S

I

L

A

I

R

S

A

A

V

L

D

S

V

S

S

H

G

A

K

T

V

L

L

L

V

L

L

L

D

D

E

E

P

P

T

S

A

S

S

G

L

L

D

D

A

P

H

L

E

M

V

E

E

N

M

V

L

F

F

Q

G

Q

V

C

L

I

R

G

Q

E

L

A

Q

R

A

Q

E

R

G

G

L

V

G

T

I

V

I

L

I

L

I

S

T

S

H

H

F

I

L

L

D

A

Q

E

V

T

Y

E

A

A

I

L

A

C

D

E

S

K

A

V

T

T

V

I

L

I

R

R

N

A

G

G

R

K

L

T

V

V

G

E

S

S

R

G

D

S

L

L

binding adenosine-5'-diphosphate: F13 (= F26), N38 (= N51), G39 (= G52), A40 (= A53), G41 (= G54), K42 (= K55), S43 (= S56), T44 (= T57)
binding adenosine-5'-triphosphate: T129 (≠ A152), S131 (= S154)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
32% identity, 42% coverage: 14:231/517 of query aligns to 2:222/648 of P75831

query
sites
P75831

T

T

P

P

L

L

L

L

E

E

A

L

R

K

S

D

I

I

T

R

K

R

S

S

F

Y

L

P

G

A

-

G

-

D

-

E

-

Q

V

V

T

E

A

V

L

L

D

K

R

G

V

I

D

S

F

L

V

D

L

I

N

Y

R

A

G

G

E

E

I

M

H

V

A

A

L

I

L

V

G

G

E

A

N

S

G

G

A

S

G

G

K
|
K

S

S

T

T

L

L

I

M

K

N

I

I

L

L

T

G

G

C

A

L

Y

D

H

K

G

A

F

T

D

S

G

G

S

T

V

Y

L

R

L

V

D

A

G

G

H

Q

A

D

I

V

A

A

P

T

A

L

-

D

-

A

-

D

S

A

V

L

A

A

E

Q

A

L

Q

R

T

R

L

E

G

H

I

F

G

G

T

F

V

I

Y

F

Q

Q

E

R

V

Y

N

H

L

L

L

L

E

S

N

H

L

L

T

T

V

A

A

E

E

Q

N

N

L

V

F

E

L

V

G

-

R

-

Q

-

P

P

R

A

R

V

F

Y

G

A

F

G

I

L

D

E

R

R

R

K

R

Q

M

R

E

L

K

L

H

R

A

A

T

Q

T

E

L

L

L

L

A

Q

R

R

Y

L

G

G

L

L

T

E

I

D

D

R

V

T

G

E

A

Y

P

Y

L

P

S

A

A

Q

Y

L

S

S

V

G

A

G

I

Q

R

Q

Q

Q

I

R

I

V

A

S

I

I

A

A

R

R

A

A

V

L

D

M

V

N

S

G

G

G

K

Q

V

V

L

I

V

L

L

A

D
|
D

E

E

P

P

T

T

A

G

S

A

L

L

D

D

A

S

H

H

E

S

V

G

E

E

M

E

L

V

F

M

G

A

V

I

L

L

R

H

Q

Q

L

L

Q

R

A

D

E

R

G

G

L

H

G

T

I

V

I

I

I

I

I

V

T

T

H

H

F

D

L

-

D

P

Q

Q

V

V

Y

A

A

A

I

Q

A

A

D

E

S

R

A

V

T

I

V

E

L

I

R

R

N

D

G

G

R

E

L

I

V

V

K47 (= K55) mutation to L: Lack of activity.
D169 (= D177) mutation to N: Lack of activity.

8k1oB Mycobacterial efflux pump, amppnp bound state
33% identity, 43% coverage: 17:236/517 of query aligns to 6:215/215 of 8k1oB

query
sites
8k1oB

L

I

E

E

A

I

R

R

S

G

I

L

T

T

K

K

S

H

F

F

L

G

G

S

V

V

T

R

A

A

L

L

D

D

R

G

V

L

D

D

F

L

V

T

L

V

N

R

R

E

G

G

E

E

I

V

H

H

A

G

L

F

L

L

G

G

E

P

N
|
N

G

G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

T

I

L

K

R

I

I

L

L

T

L

G

G

A

L

Y

V

H

K

G

A

F

D

D

G

G

G

S

S

V

V

L

R

L

L

D

L

G

G

H

G

A

-

I

-

A

D

P

P

A

W

S

T

V

D

A

A

E

V

A

D

Q

L

T

H

L

R

G

H

I

I

G

A

T

Y

V

V

Y

P

Q

G

E

D

V

V

N

T

L

L

L

W

E

P

N

S

L

L

T

T

V

G

A

G

E

E

N

T

L

I

-

D

F

L

L

L

G

A

R

R

Q

M

P

-

R

-

R

R

F

G

G

G

F

I

I

D

D

N

R

A

R

R

R

R

M

A

E

E

K

-

H

-

A

-

T

-

T

-

L

L

L

I

A

E

R

R

Y

F

G

G

L

L

T

-

I

-

D

D

V

P

G

T

A

K

P

K

L

A

S

R

A
x
T

Y

Y

S
|
S

V

K

A

G

I

N

R

R

Q

Q

I

K

I

V

A

S

I

L

A

I

R

S

A

A

V

L

D

S

V

S

S

H

G

A

K

T

V

L

L

L

V

L

L

L

D

D

E

E

P

P

T

S

A

S

S

G

L

L

D

D

A

P

H

L

E

M

V

E

E

N

M

V

L

F

F

Q

G

Q

V

C

L

I

R

G

Q

E

L

A

Q

R

A

Q

E

R

G

G

L

V

G

T

I

V

I

L

I

L

I

S

T

S

H

H

F

I

L

L

D

A

Q

E

V

T

Y

E

A

A

I

L

A

C

D

E

S

K

A

V

T

T

V

I

L

I

R

R

N

A

G

G

R

K

L

T

V

V

G

E

S

S

R

G

D

S

L

L

binding phosphoaminophosphonic acid-adenylate ester: N40 (= N51), G43 (= G54), K44 (= K55), S45 (= S56), T46 (= T57), T131 (≠ A152), S133 (= S154)

3c4jA Abc protein artp in complex with atp-gamma-s
27% identity, 42% coverage: 16:231/517 of query aligns to 3:217/242 of 3c4jA

query
sites
3c4jA

L

M

L

I

E

D

A

V

R

H

S

Q

I

L

T

K

K

K

S

S

F
|
F

L

G

G

S

V

L

T

E

A
x
V

L

L

D

K

R

G

V

I

D

N

F

V

V

H

L

I

N

R

R

E

G

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

I

C

L

L

T

N

G

L

A

L

Y

E

H

D

G

F

F

D

D

E

G

G

S

E

V

I

L

I

L

I

D

D

G

G

H

I

A

N

I

L

-

K

A

A

P

K

A

D

S

T

V

N

A

L

E

N

A

K

Q

V

T

R

L

E

G

E

I

V

G

G

T

M

V

V

Y

F

Q

Q

E

R

V

F

N

N

L

L

L

F

E

P

N

H

L

M

T

T

V

V

A

L

E

N

N

N

L

I

F

T

L

L

G

A

R

-

Q

-

P

P

R

M

R

K

F

V

G

R

F

K

I

W

D

P

R

R

R

E

R

K

M

A

E

E

K

A

H

K

A

A

T

M

T

E

L

L

L

L

A

D

R

K

Y

V

G

G

L

L

T

K

I

D

D

K

V

A

G

H

A

A

P

Y

L

P

S

D

A

S

Y

L

S

S

V

G

A

G

I

Q

R

A

Q

Q

I

R

I

V

A

A

I

I

A

A

R

R

A

A

V

L

D

A

V

M

S

E

G

P

K

K

V

I

L

M

V

L

L

F

D

D

E

E

P

P

T

T

A

S

S

A

L

L

D

D

A

P

H

E

E

M

V

V

E

G

M

E

L

V

F

L

G

S

V

V

L

M

R

K

Q

Q

L

L

Q

A

A

N

E

E

G

G

L

M

G

T

I

M

I

V

I

V

I

V

T

T

H

H

F

E

L

M

D

G

Q

F

V

A

Y

R

A

E

I

V

A

G

D

D

S

R

A

V

T

L

V

F

L

M

R

D

N

G

G

G

R

Y

L

I

V

I

binding phosphothiophosphoric acid-adenylate ester: F13 (= F26), V18 (≠ A31), S38 (≠ N51), G39 (= G52), S40 (≠ A53), G41 (= G54), K42 (= K55), S43 (= S56), T44 (= T57)

3c41J Abc protein artp in complex with amp-pnp/mg2+
27% identity, 42% coverage: 16:231/517 of query aligns to 3:217/242 of 3c41J

query
sites
3c41J

L

M

L

I

E

D

A

V

R

H

S

Q

I

L

T

K

K

K

S

S

F
|
F

L

G

G

S

V

L

T

E

A
x
V

L

L

D

K

R

G

V

I

D

N

F

V

V

H

L

I

N

R

R

E

G

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

I

C

L

L

T

N

G

L

A

L

Y

E

H

D

G

F

F

D

D

E

G

G

S

E

V

I

L

I

L

I

D

D

G

G

H

I

A

N

I

L

-

K

A

A

P

K

A

D

S

T

V

N

A

L

E

N

A

K

Q

V

T

R

L

E

G

E

I

V

G

G

T

M

V

V

Y

F

Q

Q

E

R

V

F

N

N

L

L

L

F

E

P

N

H

L

M

T

T

V

V

A

L

E

N

N

N

L

I

F

T

L

L

G

A

R

-

Q

-

P

P

R

M

R

K

F

V

G

R

F

K

I

W

D

P

R

R

R

E

R

K

M

A

E

E

K

A

H

K

A

A

T

M

T

E

L

L

L

L

A

D

R

K

Y

V

G

G

L

L

T

K

I

D

D

K

V

A

G

H

A

A

P

Y

L

P

S

D

A

S

Y

L

S

S

V

G

A

G

I

Q

R

A

Q

Q

I

R

I

V

A

A

I

I

A

A

R

R

A

A

V

L

D

A

V

M

S

E

G

P

K

K

V

I

L

M

V

L

L

F

D
|
D

E

E

P

P

T

T

A

S

S

A

L

L

D

D

A

P

H

E

E

M

V

V

E

G

M

E

L

V

F

L

G

S

V

V

L

M

R

K

Q

Q

L

L

Q

A

A

N

E

E

G

G

L

M

G

T

I

M

I

V

I

V

I

V

T

T

H

H

F

E

L

M

D

G

Q

F

V

A

Y

R

A

E

I

V

A

G

D

D

S

R

A

V

T

L

V

F

L

M

R

D

N

G

G

G

R

Y

L

I

V

I

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F26), V18 (≠ A31), S38 (≠ N51), G39 (= G52), G41 (= G54), K42 (= K55), S43 (= S56), T44 (= T57)
binding magnesium ion: S43 (= S56), D163 (= D177)

2olkA Abc protein artp in complex with adp-beta-s
27% identity, 42% coverage: 16:231/517 of query aligns to 3:217/242 of 2olkA

query
sites
2olkA

L

M

L

I

E

D

A

V

R

H

S

Q

I

L

T

K

K

K

S

S

F
|
F

L

G

G

S

V

L

T

E

A
x
V

L

L

D

K

R

G

V

I

D

N

F

V

V

H

L

I

N

R

R

E

G

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

I

C

L

L

T

N

G

L

A

L

Y

E

H

D

G

F

F

D

D

E

G

G

S

E

V

I

L

I

L

I

D

D

G

G

H

I

A

N

I

L

-

K

A

A

P

K

A

D

S

T

V

N

A

L

E

N

A

K

Q

V

T

R

L

E

G

E

I

V

G

G

T

M

V

V

Y

F

Q

Q

E

R

V

F

N

N

L

L

L

F

E

P

N

H

L

M

T

T

V

V

A

L

E

N

N

N

L

I

F

T

L

L

G

A

R

-

Q

-

P

P

R

M

R

K

F

V

G

R

F

K

I

W

D

P

R

R

R

E

R

K

M

A

E

E

K

A

H

K

A

A

T

M

T

E

L

L

L

L

A

D

R

K

Y

V

G

G

L

L

T

K

I

D

D

K

V

A

G

H

A

A

P

Y

L

P

S

D

A

S

Y

L

S

S

V

G

A

G

I

Q

R

A

Q

Q

I

R

I

V

A

A

I

I

A

A

R

R

A

A

V

L

D

A

V

M

S

E

G

P

K

K

V

I

L

M

V

L

L

F

D

D

E

E

P

P

T

T

A

S

S

A

L

L

D

D

A

P

H

E

E

M

V

V

E

G

M

E

L

V

F

L

G

S

V

V

L

M

R

K

Q

Q

L

L

Q

A

A

N

E

E

G

G

L

M

G

T

I

M

I

V

I

V

I

V

T

T

H

H

F

E

L

M

D

G

Q

F

V

A

Y

R

A

E

I

V

A

G

D

D

S

R

A

V

T

L

V

F

L

M

R

D

N

G

G

G

R

Y

L

I

V

I

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F26), V18 (≠ A31), S38 (≠ N51), G39 (= G52), S40 (≠ A53), G41 (= G54), K42 (= K55), S43 (= S56), T44 (= T57)

2oljA Abc protein artp in complex with adp/mg2+
27% identity, 42% coverage: 16:231/517 of query aligns to 3:217/242 of 2oljA

query
sites
2oljA

L

M

L

I

E

D

A

V

R

H

S

Q

I

L

T

K

K

K

S

S

F
|
F

L

G

G

S

V

L

T

E

A
x
V

L

L

D

K

R

G

V

I

D

N

F

V

V

H

L

I

N

R

R

E

G

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

I

L

K

R

I

C

L

L

T

N

G

L

A

L

Y

E

H

D

G

F

F

D

D

E

G

G

S

E

V

I

L

I

L

I

D

D

G

G

H

I

A

N

I

L

-

K

A

A

P

K

A

D

S

T

V

N

A

L

E

N

A

K

Q

V

T

R

L

E

G

E

I

V

G

G

T

M

V

V

Y

F

Q

Q

E

R

V

F

N

N

L

L

L

F

E

P

N

H

L

M

T

T

V

V

A

L

E

N

N

N

L

I

F

T

L

L

G

A

R

-

Q

-

P

P

R

M

R

K

F

V

G

R

F

K

I

W

D

P

R

R

R

E

R

K

M

A

E

E

K

A

H

K

A

A

T

M

T

E

L

L

L

L

A

D

R

K

Y

V

G

G

L

L

T

K

I

D

D

K

V

A

G

H

A

A

P

Y

L

P

S

D

A

S

Y

L

S

S

V

G

A

G

I

Q

R

A

Q

Q

I

R

I

V

A

A

I

I

A

A

R

R

A

A

V

L

D

A

V

M

S

E

G

P

K

K

V

I

L

M

V

L

L

F

D

D

E

E

P

P

T

T

A

S

S

A

L

L

D

D

A

P

H

E

E

M

V

V

E

G

M

E

L

V

F

L

G

S

V

V

L

M

R

K

Q

Q

L

L

Q

A

A

N

E

E

G

G

L

M

G

T

I

M

I

V

I

V

I

V

T

T

H

H

F

E

L

M

D

G

Q

F

V

A

Y

R

A

E

I

V

A

G

D

D

S

R

A

V

T

L

V

F

L

M

R

D

N

G

G

G

R

Y

L

I

V

I

binding adenosine-5'-diphosphate: F13 (= F26), V18 (≠ A31), S38 (≠ N51), G39 (= G52), S40 (≠ A53), G41 (= G54), K42 (= K55), S43 (= S56), T44 (= T57)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
29% identity, 43% coverage: 21:241/517 of query aligns to 9:225/285 of 4yerA

query
sites
4yerA

S

N

I

L

T

V

K

K

S

K

F
|
F

L

G

G

D

V
x
F

T

E

A

A

L

V

D

K

R

G

V

V

D

S

F

F

V

S

L

V

N

K

R

K

G

G

E

E

I

I

H

F

A

A

L

F

L

L

G

G

E

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

I

I

K

H

I

M

L

L

T

T

G

T

A

L

Y

L

H

K

G

P

F

T

D

S

G

G

S

K

V

A

L

W

L

V

D

A

G

G

H

H

A

D

I

V

-

L

-

K

A

E

P

P

A

R

S

E

V

V

A

R

E

R

A

K

Q

-

T

-

L

-

G

-

I

I

G

G

T

I

V

V

Y

F

Q

Q

E

D

V

Q

N

S

L

L

L

D

E

R

N

E

L

L

T

T

V

A

A

Y

E

E

N

N

L

M

F

Y

L

I

G

H

R

G

Q

K

P

I

R

Y

R

G

F

Y

G

G

F

-

I

-

D

-

R

G

R

E

R

K

M

L

E

K

K

K

H

R

A

I

T

L

T

E

L

L

L

L

A

E

R

F

Y

V

G

E

L

L

T

L

I

E

D

F

V

K

G

D

A

K

P

P

L

V

S

K

A
x
T

Y
x
F

S
|
S

V

G

A

G

I

M

R

A

Q

R

I

R

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L

A

E

I

I

A

A

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R

A

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V

L

D

I

V

H

S

E

G

P

K

E

V

V

L

L

V

F

L

L

D

D

E

E

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P

T

T

A

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L

L

D

D

A

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H

E

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V

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E

A

M

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K

L

M

Q

K

A

K

E

E

-

H

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N

L

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I

I

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T

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H

F

Y

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M

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D

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Y

E

A

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I

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binding adenosine-5'-diphosphate: F14 (= F26), F17 (≠ V29), N39 (= N51), G40 (= G52), G42 (= G54), K43 (= K55), T44 (≠ S56), T45 (= T57), T135 (≠ A152), F136 (≠ Y153), S137 (= S154)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
31% identity, 45% coverage: 20:250/517 of query aligns to 15:246/265 of P07821

query
sites
P07821

R

R

S

N

I

I

T

S

K

F

S

R

F

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P

G

G

V

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T

T

A

L

L

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L

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L

F

N

P

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A

G

G

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A

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L

L

I

G

G

E

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N

N

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K
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K

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T

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K50 (= K55) mutation to Q: Lack of activity.
D172 (= D177) mutation to E: Lack of activity.
E173 (= E178) mutation to A: Lack of activity.

5x40A Structure of a cbio dimer bound with amppcp (see paper)
35% identity, 42% coverage: 14:231/517 of query aligns to 2:219/280 of 5x40A

query
sites
5x40A

T

T

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x
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binding phosphomethylphosphonic acid adenylate ester: F14 (= F26), V18 (= V29), A20 (= A31), N40 (= N51), G41 (= G52), G43 (= G54), K44 (= K55), S45 (= S56), T46 (= T57), Q88 (= Q98), H139 (≠ S151), M140 (≠ A152), L141 (≠ Y153), S142 (= S154), G144 (≠ A156), Q145 (≠ I157), Q166 (≠ E178), H198 (= H210)
binding magnesium ion: S45 (= S56), Q88 (= Q98)

1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
29% identity, 42% coverage: 16:231/517 of query aligns to 4:229/254 of 1g6hA

query
sites
1g6hA

L

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N

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T

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F

I

L

I

I

I

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H

H

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L

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D

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I

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A

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I

binding adenosine-5'-diphosphate: F14 (= F26), F17 (≠ V29), N39 (= N51), G40 (= G52), G42 (= G54), K43 (= K55), S44 (= S56), T45 (= T57)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
33% identity, 35% coverage: 29:210/517 of query aligns to 20:201/615 of 5lilA

query
sites
5lilA

V

V

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A
x
V

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A

L

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G

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N
x
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|
G

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G

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K

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S