SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_176439822.1 NCBI__GCF_900188115.1:WP_176439822.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

6addA The crystal structure of rv2747 from mycobacterium tuberculosis in complex with coa and nlq (see paper)
70% identity, 93% coverage: 10:167/169 of query aligns to 6:166/168 of 6addA

query
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6addA

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binding coenzyme a: Y24 (= Y28), I28 (≠ V32), V75 (= V76), A76 (= A77), V77 (= V78), T82 (≠ R83), G83 (≠ R84), H84 (= H85), G85 (= G86), G87 (= G88), H88 (≠ D89), T110 (= T111), E112 (= E113), F115 (= F116)
binding n~2~-acetyl-l-glutamine: K27 (≠ R31), I28 (≠ V32), L30 (= L34), T74 (≠ S75), L109 (= L110), E147 (= E148)

Sites not aligning to the query:

binding coenzyme a: 1

5yo2A The crystal structure of rv2747 from mycobacterium tuberculosis in complex with acetyl coa and l-arginine (see paper)
70% identity, 93% coverage: 10:167/169 of query aligns to 6:166/168 of 5yo2A

query
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5yo2A

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binding acetyl coenzyme *a: Y24 (= Y28), I28 (≠ V32), I72 (= I73), R73 (= R74), T74 (≠ S75), V75 (= V76), V77 (= V78), T82 (≠ R83), G83 (≠ R84), H84 (= H85), G85 (= G86), G87 (= G88), H88 (≠ D89), T110 (= T111), E112 (= E113), F115 (= F116), F116 (= F117)
binding arginine: G26 (= G30), L29 (= L33), L30 (= L34), R73 (= R74), L109 (= L110), E147 (= E148)

5ygeA Arga complexed with acecoa and glutamate (see paper)
70% identity, 93% coverage: 10:167/169 of query aligns to 7:167/169 of 5ygeA

query
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binding acetyl coenzyme *a: Y25 (= Y28), I73 (= I73), V76 (= V76), A77 (= A77), V78 (= V78), T83 (≠ R83), G84 (≠ R84), H85 (= H85), G86 (= G86), G88 (= G88), H89 (≠ D89), T111 (= T111), E113 (= E113), F117 (= F117)
binding glutamic acid: K33 (= K36), E44 (= E47), H64 (= H64), R74 (= R74)

Sites not aligning to the query:

binding acetyl coenzyme *a: 1, 2

3d2pA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-arginine (see paper)
33% identity, 68% coverage: 8:122/169 of query aligns to 283:396/424 of 3d2pA

query
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3d2pA

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binding coenzyme a: L303 (≠ Y28), L349 (≠ V76), V351 (= V78), Q356 (≠ R83), D357 (≠ R84), G359 (= G86), G361 (= G88), E362 (≠ D89), E389 (= E115), W390 (≠ F116)

Sites not aligning to the query:

active site: 26
binding arginine: 13, 197, 216, 253, 266, 267, 269, 270, 271, 274, 276

4i49A Structure of ngnags bound with bisubstrate analog coa-NAG (see paper)
33% identity, 68% coverage: 8:122/169 of query aligns to 275:392/424 of 4i49A

query
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binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): I300 (≠ V32), L301 (= L33), L302 (= L34), R304 (≠ K36), A343 (≠ R74), C344 (≠ S75), L345 (≠ V76), A346 (= A77), V347 (= V78), Q352 (≠ R83), D353 (≠ R84), G355 (= G86), G357 (= G88), E358 (≠ D89), L379 (= L110), S380 (≠ T111), T383 (= T114), W386 (≠ F116), F387 (= F117)

Sites not aligning to the query:

binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): 413, 415

3d2mA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-glutamate (see paper)
33% identity, 68% coverage: 8:122/169 of query aligns to 275:392/424 of 3d2mA

query
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3d2mA

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binding coenzyme a: L345 (≠ V76), V347 (= V78), Q352 (≠ R83), D353 (≠ R84), G355 (= G86), G357 (= G88), E358 (≠ D89), S380 (≠ T111), T383 (= T114), W386 (≠ F116)
binding glutamic acid: L302 (= L34), R304 (≠ K36), A343 (≠ R74), C344 (≠ S75), L379 (= L110)

Sites not aligning to the query:

active site: 26
binding glutamic acid: 413, 415

3b8gA Crysta structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and n-acetyl-glutamate (see paper)
33% identity, 68% coverage: 8:122/169 of query aligns to 275:392/424 of 3b8gA

query
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3b8gA

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binding coenzyme a: I300 (≠ V32), L345 (≠ V76), V347 (= V78), Q352 (≠ R83), D353 (≠ R84), G355 (= G86), Y356 (≠ V87), G357 (= G88), E358 (≠ D89), S380 (≠ T111), T383 (= T114), E385 (= E115), W386 (≠ F116)
binding n-acetyl-l-glutamate: L302 (= L34), R304 (≠ K36), A343 (≠ R74), C344 (≠ S75), L379 (= L110)

Sites not aligning to the query:

active site: 26
binding n-acetyl-l-glutamate: 413, 415

2r8vA Native structure of n-acetylglutamate synthase from neisseria gonorrhoeae (see paper)
33% identity, 68% coverage: 8:122/169 of query aligns to 275:392/424 of 2r8vA

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2r8vA

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binding acetyl coenzyme *a: I300 (≠ V32), L301 (= L33), L345 (≠ V76), V347 (= V78), Q352 (≠ R83), D353 (≠ R84), G355 (= G86), G357 (= G88), E358 (≠ D89), T383 (= T114), E385 (= E115), W386 (≠ F116), F387 (= F117)

Sites not aligning to the query:

active site: 26

P0A6C5 Amino-acid acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Escherichia coli (strain K12) (see paper)
30% identity, 79% coverage: 10:142/169 of query aligns to 298:436/443 of P0A6C5

query
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P0A6C5

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Q432 (≠ M138) mutation to R: In PT2M217.

Sites not aligning to the query:

15 H→Y: In EE17.
19 Y→C: In EE51.
54 S→N: In PT2M217.
58 R→H: In EE11.
287 G→S: In PT2M216.

Query Sequence

>WP_176439822.1 NCBI__GCF_900188115.1:WP_176439822.1
MPDTHDHITIRRARIADVPGIKALVDSYSGRVLLEKELVTLYEDVQEFWVAELDGAVVGC
GALHVLWEDLAEIRSVAVDRSLRRHGVGDLIVSRLLELATELGLPRIFVLTFETEFFARH
GFVPIDGTPVSAEVYEEMRRSRDPGVAEFLDLPFAKPNTLGNTRMLLEF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory