SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_177193515.1 NCBI__GCF_900114975.1:WP_177193515.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P97084 Threonine-phosphate decarboxylase; L-threonine-O-3-phosphate decarboxylase; EC 4.1.1.81 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
35% identity, 96% coverage: 4:368/379 of query aligns to 5:364/364 of P97084

query
sites
P97084

T

N

A

S

S

A

H
|
H

G
|
G

G

G

N

N

V

I

Y

R

E

E

I

A

C

A

R

T

N

V

Y

L

G

G

W

I

S

S

I

P

D

D

E

Q

I

L

V

L

D

D

L

F

S

S

A

A

S
x
N

I

I

N

N

P

P

L

L

G

G

P

M

P

P

E

V

G

S

L

V

K

K

E

R

Y

A

L

L

L

I

S

D

R

N

F

L

G

D

E

C

I

I

V

E

H

R

Y

Y

P

P

D

D

I

A

H

D

N

Y

L

F

E

H

L

L

I

H

R

Q

A

A

L

L

A

A

E

R

Y

H

H

H

G

Q

L

V

P

P

E

A

S

S

V

W

F

I

A

L

V

A

G

G

N

N

G

G

S

E

T

T

E

E

L

S

L

I

F

F

W

T

L

V

P

A

F

S

A

G

L

L

N

K

W

P

R

R

V

A

A

M

V

I

V

V

L

T

P

P

V

G

F

F

G

A

E

E

Y

Y

L

G

R

R

S

A

L

L

E

A

N

Q

A

S

G

G

V

C

I

E

I

I

R

R

K

R

L

W

R

S

T

L

A

R

W

E

E

A

T

D

G

G

F

W

Q

Q

P

L

T

T

V

-

E

-

Q

-

L

-

D

D

A

A

L

I

V

L

H

E

A

A

S

L

N

T

P

P

D

D

V

L

-

D

-

C

V

L

I

F

L

L

T

C

N

T

P

P

G

N

S
x
N

P

P

S

T

G

G

T

L

L

L

L

P

S

E

G

R

E

P

V

L

L

L

D

Q

Y

A

I

I

K

A

W

D

S

R

V

C

R

K

K

S

R

L

Q

N

N

I

F

N

W

L

L

I

I

L

D

D

E

E

V

A

F

F

V

I

D

D

F

F

C

I

-

P

E

H

E

E

N

T

S

G

L

F

K

I

E

P

L

A

A

L

A

K

R

D

E

N

P

P

F

H

L

I

M

W

V

V

I

L

R

R

S

S

L

L

T

T

K
|
K

F

F

F

Y

S

A

L

I

P

P

G

G

L

L

R

R

I

L

G

G

Y

Y

I

L

V

V

A

N

G

S

A

D

D

D

-

A

I

A

I

M

N

A

K

R

L

M

R

R

L

R

F

Q

V

Q

P

M

P

P

W

W

S

S

V

V

N

N

I

A

F

L

A

A

Q

A

H

L

A

A

A

G

V

E

F

V

C

A

L

L

K

Q

D

D

R

S

S

A

F

W

V

Q

D

Q

K

A

T

T

L

W

S

H

F

W

F

L

D

R

R

E

E

E

K

G

K

A

R

R

V

F

K

Y

E

Q

K

A

L

L

L

C

E

Q

I

L

R

P

G

L

I

L

E

T

S

V

S

Y

P

P

F

G

A

R

A

A

N

N

Y

Y

V

L

L

L

I

L

R

R

L

C

-

E

K

R

E

E

E

D

I

I

P

D

L

L

N

-

S

-

T

-

E

-

L

-

R

-

E

Q

Q

R

M

R

L

L

R

T

H

Q

R

R

I

I

L

L

I

I

R
|
R

D

S

C

C

R

A

N

N

F

Y

E

P

G

G

L

L

S

D

D

S

R

R

Y

Y

V

Y

R
|
R

I

V

A

A

L

I

G

R

L

S

S

A

T

Q

N

N

E

E

L

R

W

L

V

L

R

A

A

A

L

L

R

R

D

N

T

V

I

L

G

T

D

G

Y

I

L

A

P

P

A

A

N

D

HG 8:9 (= HG 7:8) binding O-phospho-L-threonine
N32 (≠ S31) binding O-phospho-L-threonine
N157 (≠ S158) binding O-phospho-L-threonine
K216 (= K216) modified: N6-(pyridoxal phosphate)lysine
R323 (= R327) binding O-phospho-L-threonine
R337 (= R341) binding O-phospho-L-threonine

1lc8A Crystal structure of l-threonine-o-3-phosphate decarboxylase from s. Enterica complexed with its reaction intermediate (see paper)
36% identity, 95% coverage: 6:366/379 of query aligns to 1:356/356 of 1lc8A

query
sites
1lc8A

S

A

H
|
H

G
|
G

G

G

N

N

V

I

Y

R

E

E

I

P

C

A

R

T

N

V

Y

L

G

G

W

I

S

S

I

P

D

D

E

Q

I

L

V

L

D

D

L

F

S

S

A

A

S

N

I

I

N

N

P

P

L

L

G

G

P

M

P

P

E

V

G

S

L

V

K

K

E

R

Y

A

L

L

L

I

S

D

R

N

F

L

G

D

E

C

I

I

V

E

H

R

Y

Y

P

P

D

D

I

A

H

D

N

Y

L

F

E

H

L

L

I

H

R

Q

A

A

L

L

A

A

E

R

Y

H

H

H

G

Q

L

V

P

P

E

A

S

S

V

W

F

I

A

L

V

A

G

G

N

N

G
|
G

S
x
E

T
|
T

E

E

L

S

L

I

F

F

W

T

L

V

P

A

F

S

A

G

L

L

N

K

W

P

R

R

V

A

A

M

V

I

V

V

L

T

P

P

V

G

F
|
F

G

A

E

E

Y

Y

L

G

R

R

S

A

L

L

E

A

N

Q

A

S

G

G

V

C

I

E

I

I

R

R

K

R

L

W

R

S

T

L

A

R

W

E

E

A

T

D

G

G

F

W

Q

Q

P

L

T

T

V

-

E

-

Q

-

L

-

D

D

A

A

L

I

V

L

H

E

A

A

S

L

N

T

P

P

D

D

V

L

-

D

-

C

V

L

I

F

L

L

T

C

N

T

P

P

G

N

S
x
N

P

P

S

T

G

G

T

L

L

L

L

P

S

E

G

R

E

P

V

L

L

L

D

Q

Y

A

I

I

K

A

W

D

S

R

V

C

R

K

K

S

R

L

Q

N

N

I

F

N

W

L

L

I

I

L

D
|
D

E

E

V
x
A

F

F

V

I

D

D

F

F

C

I

-

P

E

H

E

E

N

T

S

G

L

F

K

I

E

P

L

A

A

L

A

K

R

D

E

N

P

P

F

H

L

I

M

W

V

V

I

L

R

R

S
|
S

L

L

T
|
T

K
|
K

F

F

F

Y

S

A

L

I

P

P

G

G

L

L

R
|
R

I

L

G

G

Y

Y

I

L

V

V

A

N

G

S

A

D

D

D

-

A

I

A

I

M

N

A

K

R

L

M

R

R

L

R

F

Q

V

Q

P

M

P

P

W

W

S

S

V

V

N

N

I

A

F

L

A

A

Q

A

H

L

A

A

A

G

V

E

F

V

C

A

L

L

K

Q

D

D

R

S

S

A

F

W

V

Q

D

Q

K

A

T

T

L

W

S

H

F

W

F

L

D

R

R

E

E

E

K

G

K

A

R

R

V

F

K

Y

E

Q

K

A

L

L

L

C

E

Q

I

L

R

P

G

L

I

L

E

T

S

V

S

Y

P

P

F

G

A

R

A

A

N

N

Y

Y

V

L

L

L

I

L

R

R

L

C

-

E

K

R

E

E

E

D

I

I

P

D

L

L

N

-

S

-

T

-

E

-

L

-

R

-

E

Q

Q

R

M

R

L

L

R

T

H

Q

R

R

I

I

L

L

I

I

R
|
R

D

S

C

C

R

A

N

N

F

Y

E

P

G

G

L

L

S

D

D

S

R

R

Y

Y

V

Y

R
|
R

I

V

A

A

L

I

G

R

L

S

S

A

T

Q

N

N

E

E

L

R

W

L

V

L

R

A

A

A

L

L

R

R

D

N

T

V

I

L

G

T

D

G

Y

I

L

A

P

P

binding {3-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-2-methyl-propyl}-phosphonic acid: H2 (= H7), G3 (= G8), G78 (= G83), E79 (≠ S84), T80 (= T85), F102 (= F107), N151 (≠ S158), D179 (= D186), A181 (≠ V188), S207 (= S213), T209 (= T215), K210 (= K216), R218 (= R224), R317 (= R327), R331 (= R341)

1lkcA Crystal structure of l-threonine-o-3-phosphate decarboxylase from salmonella enterica (see paper)
36% identity, 95% coverage: 7:366/379 of query aligns to 1:355/355 of 1lkcA

query
sites
1lkcA

H
|
H

G
|
G

G

G

N

N

V

I

Y

R

E

E

I

P

C

A

R

T

N

V

Y

L

G

G

W

I

S

S

I

P

D

D

E

Q

I

L

V

L

D

D

L

F

S

S

A

A

S

N

I

I

N

N

P

P

L

L

G

G

P

M

P

P

E

V

G

S

L

V

K

K

E

R

Y

A

L

L

L

I

S

D

R

N

F

L

G

D

E

C

I

I

V

E

H

R

Y

Y

P

P

D

D

I

A

H

D

N

Y

L

F

E

H

L

L

I

H

R

Q

A

A

L

L

A

A

E

R

Y

H

H

H

G

Q

L

V

P

P

E

A

S

S

V

W

F

I

A

L

V

A

G

G

N

N

G
|
G

S
x
E

T
|
T

E

E

L

S

L

I

F

F

W

T

L

V

P

A

F

S

A

G

L

L

N

K

W

P

R

R

V

A

A

M

V

I

V

V

L

T

P

P

V

G

F
|
F

G

A

E

E

Y

Y

L

G

R

R

S

A

L

L

E

A

N

Q

A

S

G

G

V

C

I

E

I

I

R

R

K

R

L

W

R

S

T

L

A

R

W

E

E

A

T

D

G

G

F

W

Q

Q

P

L

T

T

V

-

E

-

Q

-

L

-

D

D

A

A

L

I

V

L

H

E

A

A

S

L

N

T

P

P

D

D

V

L

-

D

-

C

V

L

I

F

L

L

T

C

N

T

P

P

G

N

S
x
N

P

P

S

T

G

G

T

L

L

L

L

P

S

E

G

R

E

P

V

L

L

L

D

Q

Y

A

I

I

K

A

W

D

S

R

V

C

R

K

K

S

R

L

Q

N

N

I

F

N

W

L

L

I

I

L

D
|
D

E

E

V
x
A

F
|
F

V

I

D

D

F

F

C

I

-

P

E

H

E

E

N

T

S

G

L

F

K

I

E

P

L

A

A

L

A

K

R

D

E

N

P

P

F

H

L

I

M

W

V

V

I

L

R

R

S
|
S

L

L

T
|
T

K
|
K

F

F

F

Y

S

A

L

I

P

P

G

G

L

L

R
|
R

I

L

G

G

Y

Y

I

L

V

V

A

N

G

S

A

D

D

D

-

A

I

A

I

M

N

A

K

R

L

M

R

R

L

R

F

Q

V

Q

P

M

P

P

W

W

S

S

V

V

N

N

I

A

F

L

A

A

Q

A

H

L

A

A

A

G

V

E

F

V

C

A

L

L

K

Q

D

D

R

S

S

A

F

W

V

Q

D

Q

K

A

T

T

L

W

S

H

F

W

F

L

D

R

R

E

E

E

K

G

K

A

R

R

V

F

K

Y

E

Q

K

A

L

L

L

C

E

Q

I

L

R

P

G

L

I

L

E

T

S

V

S

Y

P

P

F

G

A

R

A

A

N

N

Y

Y

V

L

L

L

I

L

R

R

L

C

-

E

K

R

E

E

E

D

I

I

P

D

L

L

N

-

S

-

T

-

E

-

L

-

R

-

E

Q

Q

R

M

R

L

L

R

T

H

Q

R

R

I

I

L

L

I

I

R
|
R

D

S

C

C

R

A

N

N

F

Y

E

P

G

G

L

L

S

D

D

S

R

R

Y

Y

V

Y

R
|
R

I

V

A

A

L

I

G

R

L

S

S

A

T

Q

N

N

E

E

L

R

W

L

V

L

R

A

A

A

L

L

R

R

D

N

T

V

I

L

G

T

D

G

Y

I

L

A

P

P

binding pyridoxal-5'-phosphate: G77 (= G83), E78 (≠ S84), T79 (= T85), F101 (= F107), D178 (= D186), A180 (≠ V188), F181 (= F189), S206 (= S213), T208 (= T215), K209 (= K216), R217 (= R224)
binding phosphate ion: H1 (= H7), G2 (= G8), N150 (≠ S158), R316 (= R327), R330 (= R341)

1lc7A Crystal structure of l-threonine-o-3-phosphate decarboxylase from s. Enterica complexed with a substrate (see paper)
36% identity, 94% coverage: 6:361/379 of query aligns to 4:354/358 of 1lc7A

query
sites
1lc7A

S

A

H
|
H

G
|
G

G

G

N

N

V

I

Y

R

E

E

I

P

C

A

R

T

N

V

Y

L

G

G

W

I

S

S

I

P

D

D

E

Q

I

L

V

L

D

D

L

F

S

S

A
|
A

S
x
N

I

I

N

N

P

P

L

L

G

G

P

M

P

P

E

V

G

S

L

V

K

K

E

R

Y

A

L

L

L

I

S

D

R

N

F

L

G

D

E

C

I

I

V

E

H

R

Y

Y

P

P

D

D

I

A

H

D

N

Y

L

F

E

H

L

L

I

H

R

Q

A

A

L

L

A

A

E

R

Y

H

H

H

G

Q

L

V

P

P

E

A

S

S

V

W

F

I

A

L

V

A

G

G

N

N

G
|
G

S
x
E

T
|
T

E

E

L

S

L

I

F

F

W

T

L

V

P

A

F

S

A

G

L

L

N

K

W

P

R

R

V

A

A

M

V

I

V

V

L

T

P

P

V

G

F
|
F

G

A

E

E

Y

Y

L

G

R

R

S

A

L

L

E

A

N

Q

A

S

G

G

V

C

I

E

I

I

R

R

K

R

L

W

R

S

T

L

A

R

W

E

E

A

T

D

G

G

F

W

Q

Q

P

L

T

T

V

-

E

-

Q

-

L

-

D

D

A

A

L

I

V

L

H

E

A

A

S

L

N

T

P

P

D

D

V

L

-

D

-

C

V

L

I

F

L

L

T

C

N

T

P

P

G

N

S
x
N

P

P

S

T

G

G

T

L

L

L

L

P

S

E

G

R

E

P

V

L

L

L

D

Q

Y

A

I

I

K

A

W

D

S

R

V

C

R

K

K

S

R

L

Q

N

N

I

F

N

W

L

L

I

I

L

D

D

E

E

V

A

F

F

V

I

D

D

F

F

C

I

-

P

E

H

E

E

N

T

S

G

L

F

K

I

E

P

L

A

A

L

A

K

R

D

E

N

P

P

F

H

L

I

M

W

V

V

I

L

R

R

S
|
S

L

L

T
|
T

K
|
K

F

F

F

Y

S

A

L

I

P

P

G

G

L

L

R
|
R

I

L

G

G

Y

Y

I

L

V

V

A

N

G

S

A

D

D

D

-

A

I

A

I

M

N

A

K

R

L

M

R

R

L

R

F

Q

V

Q

P

M

P

P

W

W

S

S

V

V

N

N

I

A

F

L

A

A

Q

A

H

L

A

A

A

G

V

E

F

V

C

A

L

L

K

Q

D

D

R

S

S

A

F

W

V

Q

D

Q

K

A

T

T

L

W

S

H

F

W

F

L

D

R

R

E

E

E

K

G

K

A

R

R

V

F

K

Y

E

Q

K

A

L

L

L

C

E

Q

I

L

R

P

G

L

I

L

E

T

S

V

S

Y

P

P

F

G

A

R

A

A

N

N

Y

Y

V

L

L

L

I

L

R

R

L

C

-

E

K

R

E

E

E

D

I

I

P

D

L

L

N

-

S

-

T

-

E

-

L

-

R

-

E

Q

Q

R

M

R

L

L

R

T

H

Q

R

R

I

I

L

L

I

I

R
|
R

D

S

C

C

R

A

N

N

F

Y

E

P

G

G

L

L

S

D

D

S

R

R

Y

Y

V

Y

R
|
R

I

V

A

A

L

I

G

R

L

S

S

A

T

Q

N

N

E

E

L

R

W

L

V

L

R

A

A

A

L

L

R

R

D

N

T

V

I

L

binding phosphate ion: G81 (= G83), E82 (≠ S84), T83 (= T85), S210 (= S213), T212 (= T215), R221 (= R224)
binding phosphothreonine: H5 (= H7), G6 (= G8), A28 (= A30), N29 (≠ S31), F105 (= F107), N154 (≠ S158), K213 (= K216), R320 (= R327), R334 (= R341)

P0DV65 L-serine phosphate decarboxylase; CobD homolog SMUL_1544; SmCobD; L-serine O-phosphate decarboxylase; L-Ser-P decarboxylase; Norcobamide biosynthesis protein SMUL_1544; Threonine phosphate decarboxylase-like enzyme; EC 4.1.1.- from Sulfurospirillum multivorans (strain DM 12446 / JCM 15788 / NBRC 109480) (see paper)
27% identity, 82% coverage: 52:363/379 of query aligns to 76:390/392 of P0DV65

query
sites
P0DV65

I

L

V

T

H

H

Y

Y

P

P

D

S

I

-

H

Q

N

Q

L

R

E

S

L

L

I

Q

R

K

A

V

L

M

A

A

E

E

-

S

Y

L

H

H

G

V

L

K

P

P

E

E

S

N

V

I

F

F

A

-

V

I

G

G

N

N

G

G

S

A

T

T

E

E

L

I

F

Q

W

M

L

L

P

L

F

Q

A

Q

L

E

N

E

W

V

R

Q

R

K

V

V

A

A

V

L

V

M

L

I

P

P

V

T

F

F

G

S

E

S

Y

Y

L

Y

R

E

S

F

L

V

-

G

E

K

N

G

A

C

G

E

V

V

I

V

I

Y

R

F

K

P

L

L

R

N

T

E

A

R

W

D

E

D

T

Y

G

S

F

F

Q

D

P

-

T

-

V

A

E

D

Q

K

L

Y

D

C

A

Q

L

F

V

I

H

E

A

N

S

E

N

Q

P

P

D

D

V

T

V

V

I

V

L

L

T

I

N

N

P

P

G

N

S

N

P

P

S

N

G

G

T

A

L

Y

L

L

S

S

G

L

E

E

V

K

L

M

D

H

Y

I

I

L

K

-

W

-

S

-

V

-

R

L

K

K

R

R

Q

L

N

A

F

F

-

V

-

P

-

R

W

I

L

I

I

I

D

D

E

E

V

S

F

F

V

I

D

H

F

F

C

A

-

Y

E

E

E

D

N

E

S

A

L

L

K

T

E

C

L

L

A

S

R

T

-

V

-

L

-

F

-

D

-

M

E

Y

P

P

F

N

L

V

M

I

V

I

I

V

R

K

S

S

L

L

T
x
S

K

K

F

D

F

F

S

G

L

I

P

A

G

G

L

V

R

R

I

L

G

G

Y

Y

I

A

V

L

A

M

G

D

A

S

D

R

I

K

I

I

N

D

K

A

L

L

R

L

L

E

F

H

V

G

P

F

P

L

W

W

S

N

V

I

N

N

I

G

F

I

A

G

Q

E

H

-

A

-

V

-

F

Y

C

C

L

L

K

-

D

-

R

R

S

L

F

F

V

V

-

R

-

E

-

D

-

F

-

L

-

K

-

R

-

Y

D

E

K

E

T

A

L

R

S

K

F

Q

F

Y

D

I

R

K

E

E

K

M

K

C

R

R

V

F

K

K

E

E

K

A

L

L

E

G

I

I

R

E

G

N

I

V

E

Y

S

V

S

Y

P

P

F

S

A

M

A

A

N

N

Y

F

V

V

L

M

I

L

R

K

L

L

K

P

E

S

E

R

I

I

P

-

L

-

N

K

S

A

T

S

E

F

L

V

R

I

E

S

Q

A

M

L

L

L

L

V

R

E

H

Y

R

G

I

I

L

Y

I

V

R

R

D

T

C

M

R

A

N

D

F

K

E

I

G

G

L

V

S

E

D

G

R

E

Y

C

V

I

R

R

I

I

A

A

L

G

G

R

L

T

S

R

S

E

T

E

N

N

E

N

L

C

W

I

V

V

R

M

A

A

L

L

R

K

D

S

T

I

I

L

G

K

D

D

S242 (≠ T215) mutation to T: Increased enzyme activity with of L-threonine phosphate and a decreased activity with L-serine phosphate. Exhibits similar activity rates with both substrates.

Sites not aligning to the query:

2:6 mutation Missing: No effect on enzyme activity with L-serine phosphate or with L-threonine phosphate.
2:11 mutation Missing: Decreased enzyme activity by about 30% compared to wild-type with L-serine phosphate. No effect on enzyme activity with L-threonine phosphate.
2:16 mutation Missing: Decreased enzyme activity by about 30% compared to wild-type with L-serine phosphate. No effect on enzyme activity with L-threonine phosphate.
2:21 mutation Missing: Decreased enzyme activity by about 30% compared to wild-type with L-serine phosphate. No effect on enzyme activity with L-threonine phosphate.
2:29 mutation Missing: Loss of enzyme activity with L-serine phosphate and with L-threonine phosphate. No effect in pyridoxal phosphate (PLP)-binding.
22:29 Required for catalytic activity
26 H→A: Significantly decreased enzyme activity. No effect in pyridoxal phosphate (PLP)-binding.

8bj3A Crystal structure of medicago truncatula histidinol-phosphate aminotransferase (hisn6) in complex with histidinol-phosphate (see paper)
25% identity, 89% coverage: 19:357/379 of query aligns to 28:356/360 of 8bj3A

query
sites
8bj3A

G

G

W

R

S

K

I

P

D

E

E

D

I

I

V

V

D

K

L

L

S

D

A

A

S

N

I

E

N

N

P

P

L

Y

G

G

P

P

E

P

G

E

L

V

K

M

E

E

Y

A

L

L

L

G

S

S

-

I

R

R

F

F

G

P

E

Y

I

V

V

-

H

-

Y
|
Y

P

P

D

D

I

P

H

E

N

S

L

R

E

R

L

L

I

R

R

A

A

A

L

L

A

A

E

Q

Y

D

H

S

G

G

L

L

P

E

E

S

S

E

V

Y

F

I

A

L

V

V

G

G

N

C

G
|
G

S
x
A

T
x
D

E

E

L

L

L

I

F

D

W

L

L

I

P

M

F

R

A

C

L

V

-

L

-

D

N

P

W

G

R

D

R

K

V

I

A

V

V

D

V

C

L

P

P

P

V

T

F
|
F

G

T

E
x
M

Y

Y

-

E

L

F

R

D

S

A

L

A

E

V

N

N

A

G

G

A

V

L

I

V

I

I

R

K

K

V

L

P

R

R

T

-

A

-

W

-

E

R

T

P

G

D

F

F

Q

S

P

L

T

N

V

V

E

E

Q

Q

L

I

D

I

A

E

L

V

V

V

H

K

A

Q

S

E

N

K

P

P

D

K

V

C

V

I

I

F

L

L

T

T

N

S

P

P

G

N

S
x
N

P

P

S

D

G

G

T

S

L

I

S

D

G

D

E

D

V

D

L

L

D

-

Y

-

I

-

K

-

W

L

S

K

V

I

R

L

K

E

R

L

Q

P

N

I

F

L

W

V

L

V

I

L

D
|
D

E

E

V
x
A

F
x
Y

V

I

D

E

F

F

C

S

E

T

E

I

N

E

S

S

L

K

K

M

E

S

L

W

A

V

A

K

R

K

E

H

P

D

F

N

L

L

M

I

V

V

I

L

R

R

S
x
T

L

F

T
x
S

K
|
K

F

R

F

A

S

G

L

L

P

A

G

G

L

L

R
|
R

I

V

G

G

Y

Y

I

G

V

A

A

F

G

P

A

L

D

S

I

I

N

K

K

Y

L

L

R

W

L

R

F

A

V

K

P

Q

P

P

W
x
Y

S

N

V

V

N

S

I

V

F

A

A

A

Q

E

H

I

A

S

A

A

V

C

F

A

C

A

L

L

K

Q

D

N

R

P

S

T

F

Y

V

L

D

E

K

N

T

V

L

K

S

D

F

A

F

L

D

V

R

K

E

E

K

R

K

G

R

R

V

L

K

F

E

D

K

L

L

L

L

K

E

A

I

V

R

P

G

F

I

L

E

K

S

P

S

F

P

P

F

S

A

H

A

S

N

N

Y

F

V

I

L

L

I

C

R

E

L

V

K

T

E

S

E

G

I

V

P

-

L

-

N

D

S

P

T

K

E

K

L

L

R

K

E

E

Q

D

M

-

L

L

L

A

R

E

H

M

R

G

I

V

L

M

I

I

R
|
R

D

H

C

Y

R

S

N

N

F

K

E

E

G

-

L

-

S

L

D

K

R

G

Y

Y

V

V

R
|
R

I

V

A

S

L

V

G

G

L

K

S

P

S

E

T

H

N

T

E

D

L

V

W

L

V

M

R

N

A

C

L

I

binding [(2~{S})-3-(1~{H}-imidazol-4-yl)-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]propyl] dihydrogen phosphate: Y63 (= Y55), G91 (= G83), A92 (≠ S84), D93 (≠ T85), F117 (= F107), M119 (≠ E109), N166 (≠ S158), D190 (= D186), A192 (≠ V188), Y193 (≠ F189), T217 (≠ S213), S219 (≠ T215), K220 (= K216), R228 (= R224), Y249 (≠ W245), R328 (= R327), R340 (= R341)

Sites not aligning to the query:

binding [(2~{S})-3-(1~{H}-imidazol-4-yl)-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]propyl] dihydrogen phosphate: 14

1fg7A Crystal structure of l-histidinol phosphate aminotransferase with pyridoxal-5'-phosphate (see paper)
26% identity, 80% coverage: 54:358/379 of query aligns to 52:350/354 of 1fg7A

query
sites
1fg7A

H

R

Y

Y

P

P

D

E

I

C

H

Q

N

P

L

K

E

A

L

V

I

I

R

E

A

N

L

Y

A

A

E

Q

Y

Y

H

A

G

G

L

V

-

K

P

P

E

E

S

Q

V

V

F

L

A

-

V

V

G

S

N

R

G
|
G

S
x
A

T
x
D

E

E

L

G

L

I

F

E

W

L

L

L

P

I

F

R

A

A

L

F

N

C

W

E

R

P

R

G

V

K

A

D

V

A

V

I

L

L

-

Y

-

C

-

P

P

P

V

T

F
x
Y

G

G

E

M

Y

Y

L

S

R

V

S

S

L

A

E

E

N

T

A

I

G

G

V

V

I

E

I

C

R

R

K

T

L

V

R

P

T

T

-

L

-

D

A

N

W

W

E

Q

T

L

G

D

F

L

Q

Q

P

G

T

I

V

S

E

D

Q

K

L

L

D

D

A

G

L

V

V

-

H

-

A

-

S

-

N

-

P

-

D

K

V

V

I

Y

L

V

T

C

N

S

P

P

G

N

S
x
N

P

P

S

T

G

G

T

Q

L

L

L

I

S

N

G

P

E

Q

V

D

L

F

D

R

Y

T

I

L

K

L

W

E

S

L

V

T

R

R

K

G

R

K

Q

A

N

-

F

I

W

V

L

V

I

A

D
|
D

E

E

V
x
A

F
x
Y

V

I

D

E

F

F

C

C

E

P

E

Q

N

A

S

S

L

L

K

A

E

G

L

W

A

L

A

A

R

E

E

Y

P

P

F

H

L

L

M

A

V

I

I

L

R

R

S
x
T

L

L

T
x
S

K
|
K

F

A

F

F

S

A

L

L

P

A

G

G

L

L

R
|
R

I

C

G

G

Y

F

I

T

V

L

A

A

G

N

A

E

D

E

I

V

I

I

N

N

K

L

L

L

R

M

L

K

F

V

V

I

P

A

P

P

W

Y

S

P

V

L

N

S

I

T

F

P

A

V

Q

A

H

D

A

I

A

A

V

A

F

Q

C

A

L

L

K

S

D

P

R

Q

S

G

F

I

V

V

D

-

K

-

T

-

L

-

S

A

F

M

F

R

D

E

R

R

E

V

K

A

K

Q

R

I

V

I

K

A

E

E

K

R

L

E

L

Y

E

L

I

I

R

A

G

A

I

L

E

K

S

E

S

I

P

P

F

C

A

V

-

E

-

Q

-

V

-

F

-

D

-

S

-

E

A

T

N

N

Y

Y

V

I

L

L

I

A

R

R

L

F

K

K

E

-

I

-

P

-

L

-

N

-

S

A

T

S

E

S

L

A

R

V

E

F

Q

K

M

S

L

L

L

W

R

D

H

Q

R

G

I

I

L

I

I

L

R

R

D

D

C

Q

R

N

N

K

F

Q

E

P

G

S

L

L

S

S

D

G

R

-

Y

C

V

L

R

R

I

I

A

T

L

V

G

G

L

T

S

R

S

E

T

E

N

S

E

Q

L

R

W

V

V

I

R

D

A

A

L

L

R

R

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G81 (= G83), A82 (≠ S84), D83 (≠ T85), Y108 (≠ F107), N155 (≠ S158), D182 (= D186), A184 (≠ V188), Y185 (≠ F189), T209 (≠ S213), S211 (≠ T215), K212 (= K216), R220 (= R224)

1fg3A Crystal structure of l-histidinol phosphate aminotransferase complexed with l-histidinol (see paper)
26% identity, 80% coverage: 54:358/379 of query aligns to 52:350/354 of 1fg3A

query
sites
1fg3A

H

R

Y

Y

P

P

D

E

I

C

H

Q

N

P

L

K

E

A

L

V

I

I

R

E

A

N

L

Y

A

A

E

Q

Y

Y

H

A

G

G

L

V

-

K

P

P

E

E

S

Q

V

V

F

L

A

-

V

V

G

S

N

R

G
|
G

S
x
A

T
x
D

E

E

L

G

L

I

F

E

W

L

L

L

P

I

F

R

A

A

L

F

N

C

W

E

R

P

R

G

V

K

A

D

V

A

V

I

L

L

-

Y

-

C

-

P

P

P

V

T

F
x
Y

G

G

E

M

Y

Y

L

S

R

V

S

S

L

A

E

E

N

T

A

I

G

G

V

V

I

E

I

C

R

R

K

T

L

V

R

P

T

T

-

L

-

D

A

N

W

W

E

Q

T

L

G

D

F

L

Q

Q

P

G

T

I

V

S

E

D

Q

K

L

L

D

D

A

G

L

V

V

-

H

-

A

-

S

-

N

-

P

-

D

K

V

V

I

Y

L

V

T

C

N

S

P

P

G

N

S
x
N

P

P

S

T

G

G

T

Q

L

L

L

I

S

N

G

P

E

Q

V

D

L

F

D

R

Y

T

I

L

K

L

W

E

S

L

V

T

R

R

K

G

R

K

Q

A

N

-

F

I

W

V

L

V

I

A

D
|
D

E

E

V
x
A

F
x
Y

V

I

D

E

F

F

C

C

E

P

E

Q

N

A

S

S

L

L

K

A

E

G

L

W

A

L

A

A

R

E

E

Y

P

P

F

H

L

L

M

A

V

I

I

L

R

R

S
x
T

L

L

T
x
S

K
|
K

F

A

F

F

S

A

L

L

P

A

G

G

L

L

R
|
R

I

C

G

G

Y

F

I

T

V

L

A

A

G

N

A

E

D

E

I

V

I

I

N

N

K

L

L

L

R

M

L

K

F

V

V

I

P

A

P

P

W

Y

S

P

V

L

N

S

I

T

F

P

A

V

Q

A

H

D

A

I

A

A

V

A

F

Q

C

A

L

L

K

S

D

P

R

Q

S

G

F

I

V

V

D

-

K

-

T

-

L

-

S

A

F

M

F

R

D

E

R

R

E

V

K

A

K

Q

R

I

V

I

K

A

E

E

K

R

L

E

L

Y

E

L

I

I

R

A

G

A

I

L

E

K

S

E

S

I

P

P

F

C

A

V

-

E

-

Q

-

V

-

F

-

D

-

S

-

E

A

T

N

N

Y

Y

V

I

L

L

I

A

R

R

L

F

K

K

E

-

I

-

P

-

L

-

N

-

S

A

T

S

E

S

L

A

R

V

E

F

Q

K

M

S

L

L

L

W

R

D

H

Q

R

G

I

I

L

I

I

L

R
|
R

D

D

C

Q

R

N

N

K

F

Q

E

P

G

S

L

L

S

S

D

G

R

-

Y

C

V

L

R
|
R

I

I

A

T

L

V

G

G

L

T

S

R

S

E

T

E

N

S

E

Q

L

R

W

V

V

I

R

D

A

A

L

L

R

R

binding phosphoric acid mono-[2-amino-3-(3h-imidazol-4-yl)-propyl]ester: D83 (≠ T85), Y108 (≠ F107), N155 (≠ S158), K212 (= K216), R320 (= R327), R333 (= R341)
binding pyridoxal-5'-phosphate: G81 (= G83), A82 (≠ S84), D83 (≠ T85), Y108 (≠ F107), N155 (≠ S158), D182 (= D186), A184 (≠ V188), Y185 (≠ F189), T209 (≠ S213), S211 (≠ T215), K212 (= K216), R220 (= R224)

Sites not aligning to the query:

binding phosphoric acid mono-[2-amino-3-(3h-imidazol-4-yl)-propyl]ester: 18, 34

1geyA Crystal structure of histidinol-phosphate aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate (see paper)
26% identity, 80% coverage: 54:357/379 of query aligns to 38:335/335 of 1geyA

query
sites
1geyA

H

R

Y

Y

P

P

D

E

I

C

H

Q

N

P

L

K

E

A

L

V

I

I

R

E

A

N

L

Y

A

A

E

Q

Y

Y

H

A

G

G

L

V

-

K

P

P

E

E

S

Q

V

V

F

L

A

-

V

V

G

S

N

R

G
|
G

S
x
A

T
x
D

E

E

L

G

L

I

F

E

W

L

L

L

P

I

F

R

A

A

L

F

N

C

W

E

R

P

R

G

V

K

A

D

V

A

V

I

L

L

-

Y

-

C

-

P

P

P

V

T

F
x
Y

G

G

E

M

Y

Y

L

S

R

V

S

S

L

A

E

E

N

T

A

I

G

G

V

V

I

E

I

C

R

R

K

T

L

V

R

P

T

T

-

L

-

D

A

N

W

W

E

Q

T

L

G

D

F

L

Q

Q

P

G

T

I

V

S

E

D

Q

K

L

L

D

D

A

G

L

V

V

-

H

-

A

-

S

-

N

-

P

-

D

K

V

V

I

Y

L

V

T

C

N

S

P

P

G

N

S
x
N

P

P

S

T

G

G

T

Q

L

L

L

I

S

N

G

P

E

Q

V

D

L

F

D

R

Y

T

I

L

K

L

W

E

S

L

V

T

R

R

K

G

R

K

Q

A

N

-

F

I

W

V

L

V

I

A

D
|
D

E

E

V
x
A

F
x
Y

V

I

D

E

F

F

C

C

E

P

E

Q

N

A

S

S

L

L

K

A

E

G

L

W

A

L

A

A

R

E

E

Y

P

P

F

H

L

L

M

A

V

I

I

L

R

R

S
x
T

L

L

T
x
S

K
|
K

F

A

F

F

S

A

L

L

P

A

G

G

L

L

R
|
R

I

C

G

G

Y

F

I

T

V

L

A

A

G

N

A

E

D

E

I

V

I

I

N

N

K

L

L

L

R

M

L

K

F

V

V

I

P

A

P

P

W

Y

S

P

V

L

N

S

I

T

F

P

A

V

Q

A

H

D

A

I

A

A

V

A

F

Q

C

A

L

L

K

S

D

P

R

Q

S

G

F

I

V

V

D

-

K

-

T

-

L

-

S

A

F

M

F

R

D

E

R

R

E

V

K

A

K

Q

R

I

V

I

K

A

E

E

K

R

L

E

L

Y

E

L

I

I

R

A

G

A

I

L

E

K

S

E

S

I

P

P

F

C

A

V

-

E

-

Q

-

V

-

F

-

D

-

S

-

E

A

T

N

N

Y

Y

V

I

L

L

I

A

R

R

L

F

K

K

E

-

I

-

P

-

L

-

N

-

S

A

T

S

E

S

L

A

R

V

E

F

Q

K

M

S

L

L

L

W

R

D

H

Q

R

G

I

I

L

I

I

L

R

R

D

D

C

Q

R

N

N

K

F

Q

E

P

G

S

L

L

S

S

D

G

R

-

Y

C

V

L

R
|
R

I

I

A

T

L

V

G

G

L

T

S

R

S

E

T

E

N

S

E

Q

L

R

W

V

V

I

R

D

A

A

L

L

binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: G67 (= G83), A68 (≠ S84), D69 (≠ T85), Y94 (≠ F107), N141 (≠ S158), D168 (= D186), A170 (≠ V188), Y171 (≠ F189), T195 (≠ S213), S197 (≠ T215), K198 (= K216), R206 (= R224), R319 (= R341)

Sites not aligning to the query:

binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: 20

3cq6A Histidinol-phosphate aminotransferase from corynebacterium glutamicum holo-form (plp covalently bound ) (see paper)
24% identity, 89% coverage: 26:361/379 of query aligns to 28:360/364 of 3cq6A

query
sites
3cq6A

V

I

D

R

L

L

S

N

A

T

S

N

I

E

N

N

P

P

L

Y

G

P

P

P

P

S

E

E

G

A

L

L

K

V

E

A

Y

D

L

L

L

V

S

A

R

T

F

V

G

D

E

K

I

I

V

A

-

T

-

E

-

L

-

N

H

R

Y

Y

P

P

D

E

I

R

H

D

N

A

L

V

E

E

L

L

I

R

R

D

A

E

L

L

A

A

E

A

Y

Y

H

I

G

T

L

K

P

Q

E

T

S

G

V

V

F

A

A

V

V

T

-

R

-

D

-

N

-

L

-

W

-

A

G

A

N

N

G

G

S

S

T

N

E

E

L

I

L

L

F

Q

W

Q

L

L

-

L

-

Q

P

A

F

F

A

G

L

G

N

P

W

G

R

R

R

T

V

A

A

L

V

G

V

F

L

Q

P

P

V

S

F

Y

G

S

E

-

Y

-

L

M

R

H

S

P

L

I

E

L

N

A

A

K

G

G

V

T

I

H

I

T

R

E

K

F

L

I

R

A

T

V

A

S

W

R

E

G

T

A

G

D

F

F

Q

R

P

I

T

D

V

M

E

D

Q

V

L

A

D

L

A

E

L

E

V

I

H

R

A

A

S

K

N

Q

P

P

D

D

V

I

V

V

I

F

L

V

T

T

N

T

P

P

G

N

S

N

P

P

S

T

G

G

T

D

L

V

L

T

S

S

G

-

E

-

V

-

L

L

D

D

Y

D

I

V

K

E

W

R

S

I

V

I

R

N

K

V

R

A

Q

P

N

G

F

I

W

V

L

I

I

V

D

D

E

E

V

A

F

Y

V

A

D

E

F

F

C

S

E

P

E

S

N

P

S

S

L

A

K

T

E

T

L

L

A

L

A

E

R

K

E

Y

P

P

F

T

-

K

L

L

M

V

V

V

I

S

R

R

S

T

L

M

T

S

K

K

F

A

F

F

S

D

L

F

P

A

G

G

L

G

R

R

I

L

G

G

Y

Y

I

F

V

V

A

A

G

N

A

P

D

A

I

F

I

I

N

D

K

A

L

V

R

M

L

L

F

V

V

R

P

L

P

P

W

Y

S

H

V

L

N

S

I

A

F

L

A

S

Q

Q

H

A

A

A

V

I

F

V

C

A

L

L

K

R

D

H

R

S

S

A

F

D

V

T

D

L

K

G

T

T

L

V

S

E

F

K

F

L

D

S

R

V

E

E

K

R

K

V

R

R

V

V

K

A

E

A

K

R

L

L

L

E

E

E

I

L

R

-

G

G

I

Y

E

A

S

V

P

P

F

S

A

E

A

S

N

N

Y

F

V

V

L

F

I

F

R

G

L

-

K

-

E

-

I

-

P

D

L

F

N

S

S

D

T

Q

E

H

L

A

R

A

E
x
W

Q

Q

M

A

L

F

L

L

R

D

H

R

R

G

I

V

L

L

I
|
I

R
|
R

D
|
D

C

V

R

-

N

-

F

-

E

-

G

G

L

I

S

A

D

G

R

-

Y

H

V

L

R
|
R

I

T

A

T

L

I

G

G

L

V

S

P

S

E

T

E

N

N

E

D

L

A

W

F

V

L

R

D

A

A

L

A

R

A

D

E

T

I

I

I

binding phosphate ion: W320 (≠ E316), I330 (= I326), R331 (= R327), R331 (= R327), D332 (= D328), R340 (= R341)

Sites not aligning to the query:

binding phosphate ion: 19, 22

3cq5B Histidinol-phosphate aminotransferase from corynebacterium glutamicum in complex with pmp (see paper)
24% identity, 89% coverage: 26:361/379 of query aligns to 30:362/366 of 3cq5B

query
sites
3cq5B

V

I

D

R

L

L

S

N

A

T

S

N

I

E

N

N

P

P

L

Y

G

P

P

P

P

S

E

E

G

A

L

L

K

V

E

A

Y

D

L

L

L

V

S

A

R

T

F

V

G

D

E

K

I

I

V

A

-

T

-

E

-

L

-

N

H

R

Y

Y

P

P

D

E

I

R

H

D

N

A

L

V

E

E

L

L

I

R

R

D

A

E

L

L

A

A

E

A

Y

Y

H

I

G

T

L

K

P

Q

E

T

S

G

V

V

F

A

A

V

V

T

-

R

-

D

-

N

-

L

-

W

-

A

G

A

N

N

G
|
G

S
|
S

T
x
N

E

E

L

I

L

L

F

Q

W

Q

L

L

-

L

-

Q

P

A

F

F

A

G

L

G

N

P

W

G

R

R

R

T

V

A

A

L

V

G

V

F

L

Q

P

P

V

S

F
x
Y

G

S

E

-

Y

-

L

M

R

H

S

P

L

I

E

L

N

A

A

K

G

G

V

T

I

H

I

T

R

E

K

F

L

I

R

A

T

V

A

S

W

R

E

G

T

A

G

D

F

F

Q

R

P

I

T

D

V

M

E

D

Q

V

L

A

D

L

A

E

L

E

V

I

H

R

A

A

S

K

N

Q

P

P

D

D

V

I

V

V

I

F

L

V

T

T

N

T

P

P

G

N

S
x
N

P

P

S

T

G

G

T

D

L

V

L

T

S

S

G

-

E

-

V

-

L

L

D

D

Y

D

I

V

K

E

W

R

S

I

V

I

R

N

K

V

R

A

Q

P

N

G

F

I

W

V

L

I

I

V

D
|
D

E

E

V
x
A

F
x
Y

V

A

D

E

F

F

C

S

E

P

E

S

N

P

S

S

L

A

K

T

E

T

L

L

A

L

A

E

R

K

E

Y

P

P

F

T

-

K

L

L

M

V

V

V

I

S

R

R

S
x
T

L

M

T
x
S

K
|
K

F

A

F

F

S

D

L

F

P

A

G

G

L

G

R
|
R

I

L

G

G

Y

Y

I

F

V

V

A

A

G

N

A

P

D

A

I

F

I

I

N

D

K

A

L

V

R

M

L

L

F

V

V

R

P

L

P

P

W

Y

S

H

V

L

N

S

I

A

F

L

A

S

Q

Q

H

A

A

A

V

I

F

V

C

A

L

L

K

R

D

H

R

S

S

A

F

D

V

T

D

L

K

G

T

T

L

V

S

E

F

K

F

L

D

S

R

V

E

E

K

R

K

V

R

R

V

V

K

A

E

A

K

R

L

L

L

E

E

E

I

L

R

-

G

G

I

Y

E

A

S

V

P

P

F

S

A

E

A

S

N

N

Y

F

V

V

L

F

I

F

R

G

L

-

K

-

E

-

I

-

P

D

L

F

N

S

S

D

T

Q

E

H

L

A

R

A

E

W

Q

Q

M

A

L

F

L

L

R

D

H

R

R

G

I

V

L

L

I

I

R

R

D

D

C

V

R

-

N

-

F

-

E

-

G

G

L

I

S

A

D

G

R

-

Y

H

V

L

R

R

I

T

A

T

L

I

G

G

L

V

S

P

S

E

T

E

N

N

E

D

L

A

W

F

V

L

R

D

A

A

L

A

R

A

D

E

T

I

I

I

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G97 (= G83), S98 (= S84), N99 (≠ T85), Y123 (≠ F107), N172 (≠ S158), D197 (= D186), A199 (≠ V188), Y200 (≠ F189), T225 (≠ S213), S227 (≠ T215), K228 (= K216), R236 (= R224)

7szpA Crystal structure of histidinol-phosphate aminotransferase from klebsiella pneumoniae subsp. Pneumoniae (strain hs11286)
26% identity, 91% coverage: 12:357/379 of query aligns to 18:349/353 of 7szpA

query
sites
7szpA

Y

Y

E

Q

I

S

C

A

R

R

N

R

Y

L

G

G

W

G

S

K

I

G

D

D

E

-

I

-

V

V

D

W

L

L

S

N

A

A

S

N

I

E

N

F

P

P

L

T

G

A

P

V

P

A

E

F

G

Q

L

L

K

T

E

E

Y

Q

L

T

L

L

S

N

R

R

F

-

G

-

E

-

I

-

V

-

H

-

Y

Y

P

P

D

E

I

P

H

Q

N

P

L

K

E

A

L

V

I

I

R

E

A

S

L

Y

A

A

E

R

Y

Y

H

A

G

E

L

V

-

K

P

P

E

E

S

Q

V

V

F

L

A

-

V

V

G

S

N

R

G
|
G

S
x
A

T
x
D

E

E

L

G

L

I

F

E

W

L

L

L

P

I

F

R

A

A

L

F

N

C

W

E

R

P

R

G

V

E

A

D

V

A

V

V

L

L

-

Y

-

C

-

P

P

P

V

T

F
x
Y

G

G

E

M

Y

Y

L

S

R

V

S

S

L

A

E

E

N

T

A

I

G

G

V

V

I

E

I

C

R

R

K

T

L

V

R

P

T

T

-

L

-

A

A

D

W

W

E

Q

T

L

G

D

F

L

Q

P

P

G

T

I

V

E

E

A

Q

R

L

L

D

D

A

G

L

V

V

-

H

-

A

-

S

-

N

-

P

-

D

K

V

V

I

F

L

V

T

C

N

S

P

P

G

N

S

N

P

P

S

T

G

G

T

Q

L

I

S

D

G

P

E

Q

V

S

L

M

D

R

Y

D

I

L

K

L

W

E

S

M

V

T

R

R

K

G

R

K

Q

A

N

-

F

I

W

V

L

V

I

A

D
|
D

E

E

V
x
A

F
x
Y

V

I

D

E

F

F

C

C

E

P

E

Q

N

A

S

T

L

L

K

A

E

G

L

W

A

L

A

S

R

D

E

Y

P

P

F

H

L

L

M

V

V

V

I

L

R

R

S
x
T

L

L

T
x
S

K
|
K

F

A

F

F

S

A

L

L

P

A

G

G

L

L

R
|
R

I

C

G

G

Y

F

I

T

V

L

A

A

G

N

A

A

D

E

I

V

I

I

N

N

K

V

L

L

R

L

L

K

F

V

V

I

P

A

P

P

W

Y

S

P

V

L

N

S

I

T

F

P

A

V

Q

A

H

D

A

I

A

A

V

A

F

Q

C

A

L

L

K

S

D

P

R

E

S

G

F

I

V

A

-

A

-

M

-

R

D

Q

K

R

T

V

L

A

S

Q

F

I

F

L

D

D

R

-

E

E

K

R

R

Y

V

L

K

V

E

E

K

Q

L

L

L

R

E

G

I

I

R

A

G

C

I

V

E

E

S

Q

S

V

-

F

P

D

F

S

A

E

A

T

N

N

Y

Y

V

V

L

L

I

A

R

R

L

-

K

-

E

-

I

-

P

-

L

I

N

T

S

A

T

S

E

S

L

A

R

V

E

F

Q

K

M

S

L

L

L

W

R

D

H

Q

R

G

I

I

L

I

I

L

R

R

D

D

C

Q

R

N

N

K

F

Q

E

P

G

S

L

L

S

S

D

G

R

-

Y

C

V

L

R

R

I

I

A

T

L

I

G

G

L

T

S

R

S

A

T

E

N

S

E

Q

L

R

W

V

V

I

R

D

A

A

L

L

binding pyridoxal-5'-phosphate: G81 (= G83), A82 (≠ S84), D83 (≠ T85), Y108 (≠ F107), D182 (= D186), A184 (≠ V188), Y185 (≠ F189), T209 (≠ S213), S211 (≠ T215), K212 (= K216), R220 (= R224)

Q0ZQ41 CMP-5'-(3-aminopropyl)phosphonate synthase; EC 2.7.7.-; EC 4.1.1.- from Streptomyces rubellomurinus (strain ATCC 31215) (see paper)
29% identity, 81% coverage: 37:343/379 of query aligns to 291:593/628 of Q0ZQ41

query
sites
Q0ZQ41

P

P

E

A

G

E

L

M

K

M

E

E

Y

I

L

L

L

R

S

S

R

D

F

F

G

D

E

A

I

V

V

L

-

R

H

N

Y

Y

P

P

D

S

I

A

H

H

N

A

-

P

-

L

L

T

E

E

L

L

I

A

R

A

A

T

L

I

A

A

E

A

Y

R

H

R

G

-

L

-

P

P

E

E

S

E

V

V

F

V

A

-

V

L

G

A

N

N

G

G

S

S

T

S

E

E

L

I

F

K

W

I

L

L

P

A

-

R

F

L

A

R

L

G

N

N

W

W

R

-

V

-

A

T

V

V

V

P

L

V

P

P

V

G

F

F

G

N

E

E

Y

Y

L

-

R

-

S

-

L

-

E

E

N

N

A

V

G

V

V

G

I

A

I

E

R

R

K

V

L

H

R

R

T

Y

A

Q

W

L

E

D

T

A

-

P

G

D

F

F

Q

R

P

L

T

P

V

V

E

E

Q

D

L

Y

D

A

A

A

L

F

V

V

H

R

A

R

S

S

N

G

P

V

D

D

V

T

V

A

I

V

L

V

T

V

N

S

P

P

G

N

S

N

P

P

S

T

-

S

-

V

G

G

T

V

L

P

L

L

S

A

G

D

-

L

-

R

E

R

V

L

L

A

D

D

Y

L

I

V

K

G

W

P

S

D

V

V

R

-

K

-

R

-

Q

-

N

-

F

L

W

L

L

V

I

I

D

D

E

E

V

S

F

F

V

V

D

D

F

F

C

A

E

P

E

A

-

P

-

I

N

A

S

S

L

I

K

G

E

P

L

F

A

L

A

D

R

R

E

H

P

R

F

N

L

V

M

L

V

L

I

L

R

K

S

S

L

I

T

S

K
|
K

F

V

F

Y

S

G

L

V

P

G

G

G

L

I

R

R

I

L

G

G

Y

Y

-

A

I

A

V

T

A

A

G

D

A

T

D

E

I

L

I

A

N

R

K

T

L

L

R

R

L

A

F

E

V

L

P

P

P

I

W

W

S

D

V

I

N

N

I

G

F

F

A

A

Q

E

H

E

-

F

A

L

A

R

V

V

F

L

C

P

L

H

K

F

D

R

R

R

S

A

F

F

V

A

D

D

K

S

T

C

L

R

S

Q

F

-

F

M

D

R

R

Q

E

N

K

T

K

L

R

A

V

L

K

A

E

E

K

G

L

L

L

A

E

A

I

L

R

P

G

G

I

I

E

R

S

V

P

P

F

P

A

D

A

A

N

N

Y

F

V

V

L

F

I

V

R

E

L

L

K

T

E

G

I

I

P

-

L

-

N

R

S

A

T

P

E

E

L

L

R

A

E

H

Q

E

M

L

L

F

L

R

R

R

H

F

R

R

I

I

L

L

I

T

R

K

D

E

C

C

-

S

-

G

R

K

N

S

F

M

E

P

G

G

L

-

S

G

D

D

R

A

Y

Y

V

L

R

R

I

V

A

S

K466 (= K216) mutation to A: Lack of decarboxylase activity, produces only CMP-5'-2APn.

Sites not aligning to the query:

37 K→A: Strong decrease in nucleotide transferase activity.

2f8jA Crystal structure of histidinol-phosphate aminotransferase (ec 2.6.1.9) (imidazole acetol-phosphate transferase) (tm1040) from thermotoga maritima at 2.40 a resolution
26% identity, 87% coverage: 28:357/379 of query aligns to 25:332/335 of 2f8jA

query
sites
2f8jA

L

L

S

A

A

L

S

N

I

E

N

N

P

P

L

F

G

P

P

F

P

P

E

E

G

D

L

L

K

V

E

D

Y

E

L

V

L

F

S

R

R

R

F

L

G

N

E

S

I

D

-

A

-

L

-

R

V

I

H

Y

Y

Y

-

D

-

S

P

P

D

D

I

E

H

E

N

L

L

I

E

E

L

K

I

I

R

L

A

S

L

Y

A

L

E

D

Y

T

H

D

G

F

L

L

P

S

E

K

S

N

V

N