SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_198002428.1 NCBI__GCF_000017185.1:WP_198002428.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
55% identity, 100% coverage: 1:390/390 of query aligns to 3:391/392 of P33221

query
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P33221

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EL 22:23 (= EL 20:21) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
E82 (= E80) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
R114 (= R112) binding ATP
K155 (= K153) binding ATP
SSGKGQ 160:165 (= SSGKGQ 158:163) binding ATP
G162 (= G160) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
K179 (≠ N179) modified: N6-acetyllysine
EGVV 195:198 (≠ EEFI 195:198) binding ATP
E203 (= E203) binding ATP
E267 (= E267) binding Mg(2+)
E279 (= E279) binding Mg(2+)
D286 (= D286) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
K355 (= K354) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
RR 362:363 (= RR 361:362) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
55% identity, 100% coverage: 1:390/390 of query aligns to 2:388/389 of 1kjiA

query
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1kjiA

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active site: E114 (= E113), K154 (= K153), S159 (= S158), G161 (= G160), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R362)
binding phosphomethylphosphonic acid adenylate ester: R113 (= R112), I152 (= I151), K154 (= K153), S159 (= S158), S160 (= S159), G161 (= G160), Q164 (= Q163), E192 (= E195), V195 (≠ I198), E200 (= E203), Q222 (= Q225), E264 (= E267), F266 (= F269), E276 (= E279)
binding magnesium ion: E264 (= E267), E276 (= E279)

1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
55% identity, 100% coverage: 1:390/390 of query aligns to 2:388/389 of 1ez1A

query
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1ez1A

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active site: E114 (= E113), K154 (= K153), S159 (= S158), G161 (= G160), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R362)
binding phosphoaminophosphonic acid-adenylate ester: R113 (= R112), I152 (= I151), K154 (= K153), S159 (= S158), S160 (= S159), G161 (= G160), E192 (= E195), V194 (≠ F197), V195 (≠ I198), F197 (= F200), E200 (= E203), Q222 (= Q225), E264 (= E267), F266 (= F269), E276 (= E279)
binding glycinamide ribonucleotide: G20 (= G19), E21 (= E20), L22 (= L21), E81 (= E80), I82 (= I81), S160 (= S159), D283 (= D286), K352 (= K354), R359 (= R361), R360 (= R362)
binding magnesium ion: E264 (= E267), E276 (= E279)

1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
55% identity, 100% coverage: 1:390/390 of query aligns to 2:388/389 of 1eyzA

query
sites
1eyzA

M

L

L

L

G

G

T

T

P

A

L

L

F

R

S

P

N

A

G

A

T

T

K

R

I

V

L

M

L

L

G

G

S

S

G

G

E

E

L

L

G

G

K

K

E

E

V

V

I

A

I

I

E

E

A

C

Q

Q

R

R

L

L

G

G

I

V

E

E

C

V

V

I

A

A

V

V

D

D

S

R

Y

Y

D

A

N

D

A

A

P

P

A

A

M

M

Q

H

V

V

A

A

H

H

R

R

S

S

Y

H

V

V

I

I

D

N

M

M

K

L

D

D

G

G

D

D

A

A

L

L

K

R

A

R

I

V

L

V

E

E

K

L

E

E

V

K

P

P

D

H

Y

Y

I

I

I

V

P

P

E

E

I

I

E

E

A

A

I

I

N

A

T

T

D

D

I

M

L

L

I

I

E

Q

M

L

E

E

F

E

G

G

H

L

Q

N

V

V

P

P

T

C

A

A

N

R

A

A

A

T

K

K

I

L

T

T

M

M

D

N

R
|
R

E
|
E

G

G

I

I

R

R

L

L

A

A

S

A

E

E

Q

E

L

L

N

Q

I

L

P

P

T

T

A

S

K

T

Y

Y

E

R

F

F

A

A

E

D

S

S

L

E

E

S

E

L

L

F

K

R

K

E

A

A

V

V

K

A

K

D

I

I

G

G

A

Y

P

P

C

C

I
|
I

V

V

K
|
K

P

P

I

V

M

M

S

S

S
|
S

G
|
G

K

K

G

G

Q
|
Q

S

T

T

F

V

I

K

R

S

S

E

A

K

-

D

-

E

E

D

Q

I

L

E

A

Y

Q

A

A

W

W

N

K

Y

Y

A

A

Q

Q

A

Q

G

G

A

G

R

R

G

-

I

-

G

A

K

G

K

R

V

V

I

I

V

V

E
|
E

E

G

F

V

I
x
V

N

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

A

V

R

S

T

A

A

V

H

D

G

G

T

V

V

H

Y

F

C

C

P

A

P

P

I

V

G

G

H

H

I

R

Q

Q

D

E

G

D

G

G

D

D

Y

Y

I

R

E

E

S

S

W

W

Q

Q

P

P

H

Q

P

Q

M

M

S

S

E

P

E

L

M

A

I

L

K

E

K

R

A

A

Q

Q

E

E

M

I

A

A

N

R

K

K

I

V

T

V

T

L

V

A

L

L

G

G

N

Y

G

G

I

L

F

F

G

G

V

V

E
|
E

L

L

F
|
F

I

V

R

C

G

G

D

D

E

E

V

V

I

I

F

F

S

S

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

M

L

I

I

T

S

Q

Q

N

D

M

L

S

S

E

E

F

F

E

A

I

L

H

H

L

V

R

R

S

A

I

F

L

L

G

G

L

L

P

P

V

V

-

G

D

G

I

I

Q

R

L

Q

I

Y

T

G

A

P

G

A

A

A

S

S

S

A

V

V

I

I

K

L

S

P

K

Q

I

L

H

T

K

S

Y

Q

A

N

P

V

Q

T

Y

F

D

D

-

N

I

V

S

Q

D

N

A

A

V

V

K

G

V

A

P

-

N

D

T

L

K

Q

L

I

R

R

I

L

F

F

G

G

K

K

P

P

L

E

A

I

K

D

V

G

G

S

R

R

R
|
R

M

L

G

G

V

V

A

A

L

L

A

A

S

T

A

A

N

E

T

S

V

V

E

V

E

D

A

A

R

I

K

E

N

R

A

A

K

K

K

H

C

A

A

A

N

G

G

Q

V

V

I

K

I

V

R

Q

active site: E114 (= E113), K154 (= K153), S159 (= S158), G161 (= G160), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R362)
binding phosphoaminophosphonic acid-adenylate ester: R113 (= R112), I152 (= I151), K154 (= K153), S159 (= S158), S160 (= S159), G161 (= G160), Q164 (= Q163), E192 (= E195), V195 (≠ I198), F197 (= F200), E200 (= E203), E264 (= E267), F266 (= F269), E276 (= E279)
binding magnesium ion: E264 (= E267), E276 (= E279)

1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
55% identity, 100% coverage: 1:390/390 of query aligns to 2:385/386 of 1kjjA

query
sites
1kjjA

M

L

L

L

G

G

T

T

P

A

L

L

F

R

S

P

N

A

G

A

T

T

K

R

I

V

L

M

L

L

G

G

S

S

G

G

E

E

L

L

G

G

K

K

E

E

V

V

I

A

I

I

E

E

A

C

Q

Q

R

R

L

L

G

G

I

V

E

E

C

V

V

I

A

A

V

V

D

D

S

R

Y

Y

D

A

N

D

A

A

P

P

A

A

M

M

Q

H

V

V

A

A

H

H

R

R

S

S

Y

H

V

V

I

I

D

N

M

M

K

L

D

D

G

G

D

D

A

A

L

L

K

R

A

R

I

V

L

V

E

E

K

L

E

E

V

K

P

P

D

H

Y

Y

I

I

I

V

P

P

E

E

I

I

E

E

A

A

I

I

N

A

T

T

D

D

I

M

L

L

I

I

E

Q

M

L

E

E

F

E

G

G

H

L

Q

N

V

V

P

P

T

C

A

A

N

R

A

A

A

T

K

K

I

L

T

T

M

M

D

N

R
|
R

E
|
E

G

G

I

I

R

R

L

L

A

A

S

A

E

E

Q

E

L

L

N

Q

I

L

P

P

T

T

A

S

K

T

Y

Y

E

R

F

F

A

A

E

D

S

S

L

E

E

S

E

L

L

F

K

R

K

E

A

A

V

V

K

A

K

D

I

I

G

G

A

Y

P

P

C

C

I
|
I

V

V

K
|
K

P

P

I

V

M

M

S

S

S
|
S

G
|
G

K

K

G

G

Q
|
Q

S

T

T

F

V

I

K

R

S

S

E

A

K

-

D

-

E

E

D

Q

I

L

E

A

Y

Q

A

A

W

W

N

K

Y

Y

A

A

Q

Q

A

Q

G

G

A

G

R

R

G

-

I

-

G

-

K

-

V

V

I

I

V

V

E
|
E

E

G

F

V

I
x
V

N

K

F

F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

A

V

R

S

T

A

A

V

H

D

G

G

T

V

V

H

Y

F

C

C

P

A

P

P

I

V

G

G

H

H

I

R

Q
|
Q

D

E

G

D

G

G

D

D

Y

Y

I

R

E

E

S

S

W

W

Q

Q

P

P

H

Q

P

Q

M

M

S

S

E

P

E

L

M

A

I

L

K

E

K

R

A

A

Q

Q

E

E

M

I

A

A

N

R

K

K

I

V

T

V

T

L

V

A

L

L

G

G

N

Y

G

G

I

L

F

F

G

G

V

V

E
|
E

L

L

F
|
F

I

V

R

C

G

G

D

D

E

E

V

V

I

I

F

F

S

S

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

M

L

I

I

T

S

Q

Q

N

D

M

L

S

S

E

E

F

F

E

A

I

L

H

H

L

V

R

R

S

A

I

F

L

L

G

G

L

L

P

P

V

V

-

G

D

G

I

I

Q

R

L

Q

I

Y

T

G

A

P

G

A

A

A

S

S

S

A

V

V

I

I

K

L

S

P

K

Q

I

L

H

T

K

S

Y

Q

A

N

P

V

Q

T

Y

F

D

D

-

N

I

V

S

Q

D

N

A

A

V

V

K

G

V

A

P

-

N

D

T

L

K

Q

L

I

R

R

I

L

F

F

G

G

K

K

P

P

L

E

A

I

K

D

V

G

G

S

R

R

R
|
R

M

L

G

G

V

V

A

A

L

L

A

A

S

T

A

A

N

E

T

S

V

V

E

V

E

D

A

A

R

I

K

E

N

R

A

A

K

K

K

H

C

A

A

A

N

G

G

Q

V

V

I

K

I

V

R

Q

active site: E114 (= E113), K154 (= K153), S159 (= S158), G161 (= G160), E261 (= E267), E273 (= E279), D280 (= D286), T281 (= T287), R357 (= R362)
binding phosphothiophosphoric acid-adenylate ester: R113 (= R112), I152 (= I151), K154 (= K153), S159 (= S158), S160 (= S159), G161 (= G160), Q164 (= Q163), E189 (= E195), V192 (≠ I198), E197 (= E203), Q219 (= Q225), E261 (= E267), F263 (= F269), E273 (= E279)
binding magnesium ion: E261 (= E267), E273 (= E279)

1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
55% identity, 100% coverage: 1:390/390 of query aligns to 2:385/386 of 1kj8A

query
sites
1kj8A

M

L

L

L

G

G

T

T

P

A

L

L

F

R

S

P

N

A

G

A

T

T

K

R

I

V

L

M

L

L

G

G

S

S

G
|
G

E
|
E

L
|
L

G

G

K

K

E

E

V

V

I

A

I

I

E

E

A

C

Q

Q

R

R

L

L

G

G

I

V

E

E

C

V

V

I

A

A

V

V

D

D

S

R

Y

Y

D

A

N

D

A

A

P

P

A

A

M

M

Q

H

V

V

A

A

H

H

R

R

S

S

Y

H

V

V

I

I

D

N

M

M

K

L

D

D

G

G

D

D

A

A

L

L

K

R

A

R

I

V

L

V

E

E

K

L

E

E

V

K

P

P

D

H

Y

Y

I

I

I

V

P

P

E
|
E

I
|
I

E

E

A

A

I

I

N

A

T

T

D

D

I

M

L

L

I

I

E

Q

M

L

E

E

F

E

G

G

H

L

Q

N

V

V

P

P

T

C

A

A

N

R

A

A

A

T

K

K

I

L

T

T

M

M

D

N

R
|
R

E
|
E

G

G

I

I

R

R

L

L

A

A

S

A

E

E

Q

E

L

L

N

Q

I

L

P

P

T

T

A

S

K

T

Y

Y

E

R

F

F

A

A

E

D

S

S

L

E

E

S

E

L

L

F

K

R

K

E

A

A

V

V

K

A

K

D

I

I

G

G

A

Y

P

P

C

C

I
|
I

V

V

K
|
K

P

P

I

V

M

M

S

S

S
|
S

G
|
G

K

K

G

G

Q
|
Q

S

T

T

F

V

I

K

R

S

S

E

A

K

-

D

-

E

E

D

Q

I

L

E

A

Y

Q

A

A

W

W

N

K

Y

Y

A

A

Q

Q

A

Q

G

G

A

G

R

R

G

-

I

-

G

-

K

-

V

V

I

I

V

V

E
|
E

E

G

F

V

I
x
V

N

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

A

V

R

S

T

A

A

V

H

D

G

G

T

V

V

H

Y

F

C

C

P

A

P

P

I

V

G

G

H

H

I

R

Q
|
Q

D

E

G

D

G
|
G

D

D

Y

Y

I

R

E

E

S

S

W

W

Q

Q

P

P

H

Q

P

Q

M

M

S

S

E

P

E

L

M

A

I

L

K

E

K

R

A

A

Q

Q

E

E

M

I

A

A

N

R

K

K

I

V

T

V

T

L

V

A

L

L

G

G

N

Y

G

G

I

L

F

F

G

G

V

V

E
|
E

L

L

F
|
F

I

V

R

C

G

G

D

D

E

E

V

V

I

I

F

F

S

S

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

M

L

I

I

T

S

Q

Q

N

D

M

L

S

S

E

E

F

F

E

A

I

L

H

H

L

V

R

R

S

A

I

F

L

L

G

G

L

L

P

P

V

V

-

G

D

G

I

I

Q

R

L

Q

I

Y

T

G

A

P

G

A

A

A

S

S

S

A

V

V

I

I

K

L

S

P

K

Q

I

L

H

T

K

S

Y

Q

A

N

P

V

Q

T

Y

F

D

D

-

N

I

V

S

Q

D

N

A

A

V

V

K

G

V

A

P

-

N

D

T

L

K

Q

L

I

R

R

I

L

F

F

G

G

K
|
K

P

P

L

E

A

I

K

D

V

G

G

S

R
|
R

M

L

G

G

V

V

A

A

L

L

A

A

S

T

A

A

N

E

T

S

V

V

E

V

E

D

A

A

R

I

K

E

N

R

A

A

K

K

K

H

C

A

A

A

N

G

G

Q

V

V

I

K

I

V

R

Q

active site: E114 (= E113), K154 (= K153), S159 (= S158), G161 (= G160), E261 (= E267), E273 (= E279), D280 (= D286), T281 (= T287), R357 (= R362)
binding adenosine-5'-triphosphate: R113 (= R112), I152 (= I151), K154 (= K153), S159 (= S158), S160 (= S159), G161 (= G160), Q164 (= Q163), E189 (= E195), V192 (≠ I198), F194 (= F200), E197 (= E203), Q219 (= Q225), G222 (= G228), E261 (= E267), F263 (= F269), E273 (= E279)
binding glycinamide ribonucleotide: G20 (= G19), E21 (= E20), L22 (= L21), E81 (= E80), I82 (= I81), S160 (= S159), D280 (= D286), K349 (= K354), R356 (= R361)
binding magnesium ion: E261 (= E267), E273 (= E279)

1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
54% identity, 100% coverage: 1:390/390 of query aligns to 2:387/388 of 1kjqA

query
sites
1kjqA

M

L

L

L

G

G

T

T

P

A

L

L

F

R

S

P

N

A

G

A

T

T

K

R

I

V

L

M

L

L

G

G

S

S

G

G

E

E

L

L

G

G

K

K

E

E

V

V

I

A

I

I

E

E

A

C

Q

Q

R

R

L

L

G

G

I

V

E

E

C

V

V

I

A

A

V

V

D

D

S

R

Y

Y

D

A

N

D

A

A

P

P

A

A

M

M

Q

H

V

V

A

A

H

H

R

R

S

S

Y

H

V

V

I

I

D

N

M

M

K

L

D

D

G

G

D

D

A

A

L

L

K

R

A

R

I

V

L

V

E

E

K

L

E

E

V

K

P

P

D

H

Y

Y

I

I

I

V

P

P

E

E

I

I

E

E

A

A

I

I

N

A

T

T

D

D

I

M

L

L

I

I

E

Q

M

L

E

E

F

E

G

G

H

L

Q

N

V

V

P

P

T

C

A

A

N

R

A

A

A

T

K

K

I

L

T

T

M

M

D

N

R
|
R

E
|
E

G

G

I

I

R

R

L

L

A

A

S

A

E

E

Q

E

L

L

N

Q

I

L

P

P

T

T

A

S

K

T

Y

Y

E

R

F

F

A

A

E

D

S

S

L

E

E

S

E

L

L

F

K

R

K

E

A

A

V

V

K

A

K

D

I

I

G

G

A

Y

P

P

C

C

I
|
I

V

V

K
|
K

P

P

I

V

M

M

S

S

S

-

G

-

K

K

G

G

Q

Q

S

T

T

F

V

I

K

R

S

S

E

A

K

-

D

-

E

E

D

Q

I

L

E

A

Y

Q

A

A

W

W

N

K

Y

Y

A

A

Q

Q

A

Q

G

G

A

G

R

R

G

A

I

G

G

A

K

G

K

R

V

V

I

I

V

V

E
|
E

E

G

F
x
V

I
x
V

N

K

F
|
F

D

D

Y

F

E
|
E

I

I

T

T

L

L

T

T

A

V

R

S

T

A

A

V

H

D

G

G

T

V

V

H

Y

F

C

C

P

A

P

P

I

V

G

G

H

H

I

R

Q
|
Q

D

E

G

D

G

G

D

D

Y

Y

I

R

E

E

S

S

W

W

Q

Q

P

P

H

Q

P

Q

M

M

S

S

E

P

E

L

M

A

I

L

K

E

K

R

A

A

Q

Q

E

E

M

I

A

A

N

R

K

K

I

V

T

V

T

L

V

A

L

L

G

G

N

Y

G

G

I

L

F

F

G

G

V

V

E
|
E

L

L

F
|
F

I

V

R

C

G

G

D

D

E

E

V

V

I

I

F

F

S

S

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

M

L

I

I

T

S

Q

Q

N

D

M

L

S

S

E

E

F

F

E

A

I

L

H

H

L

V

R

R

S

A

I

F

L

L

G

G

L

L

P

P

V

V

-

G

D

G

I

I

Q

R

L

Q

I

Y

T

G

A

P

G

A

A

A

S

S

S

A

V

V

I

I

K

L

S

P

K

Q

I

L

H

T

K

S

Y

Q

A

N

P

V

Q

T

Y

F

D

D

-

N

I

V

S

Q

D

N

A

A

V

V

K

G

V

A

P

-

N

D

T

L

K

Q

L

I

R

R

I

L

F

F

G

G

K

K

P

P

L

E

A

I

K

D

V

G

G

S

R

R

R
|
R

M

L

G

G

V

V

A

A

L

L

A

A

S

T

A

A

N

E

T

S

V

V

E

V

E

D

A

A

R

I

K

E

N

R

A

A

K

K

K

H

C

A

A

A

N

G

G

Q

V

V

I

K

I

V

R

Q

active site: E114 (= E113), K154 (= K153), E263 (= E267), E275 (= E279), D282 (= D286), T283 (= T287), R359 (= R362)
binding adenosine-5'-diphosphate: R113 (= R112), I152 (= I151), K154 (= K153), E191 (= E195), V193 (≠ F197), V194 (≠ I198), F196 (= F200), E199 (= E203), Q221 (= Q225), F265 (= F269), E275 (= E279)
binding magnesium ion: E263 (= E267), E275 (= E279)

O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
50% identity, 100% coverage: 2:390/390 of query aligns to 8:411/430 of O58056

query
sites
O58056

L

L

G

G

T

T

P

A

L

T

F

T

S

D

N

S

G

A

T

Q

K

K

I

I

L

L

G

G

S

S

G

G

E

E

L

L

G

G

K

K

E

E

V

I

I

A

I

I

E

E

A

A

Q

Q

R

R

L

L

G

G

I

V

E

E

C

V

V

V

A

A

V

V

D

D

S

R

Y

Y

D

A

N

N

A

A

P

P

A

A

M

M

Q

Q

V

V

A

A

H

H

R

R

S

S

Y

Y

V

V

I

G

D

N

M

M

K

M

D

D

G

K

D

D

A

F

L

L

K

W

A

S

I

V

L

V

E

E

K

R

E

E

V

K

P

P

D

D

Y

A

I

I

P

P

E

E

I

I

E

E

A

A

I

I

N

N

T

L

D

D

I

A

L

L

I

F

E

E

M

F

E

E

E

K

F

D

G

G

H

Y

Q

F

V

V

P

P

T

N

A

A

N

R

A

A

A

T

K

W

I

I

T

A

M

M

D

H

R
|
R

E

E

G

R

I

L

R

R

R

E

L

T

A

L

S

V

E

K

Q

E

L

A

N

K

I

V

P

P

T

T

A

S

K

R

Y

Y

E

M

F

Y

A

A

E

T

S

T

L

L

E

D

E

E

L

L

K

Y

K

E

A

A

V

C

K

E

K

K

I

I

G

G

A

Y

P

P

C

C

I

H

V

T

K
|
K

P

A

I

I

M

M

S

S

G

G

K

K

G

G

Q

S

S

Y

T

F

V

V

K

K

S

G

E

P

K

-

D

-

E

E

D

D

I

I

E

P

Y

K

A

A

W

W

N

E

Y

E

A

A

Q

K

A

T

G

K

A

A

R

R

G

G

I

S

G

A

K

E

K

K

V

I

I

I

V

V

E
|
E

F
x
H

I
|
I

N

D

F

F

D

D

Y

V

E
|
E

I

V

T

T

L

E

L

L

T

A

A

V

R

R

-

H

-

F

T

D

A

E

H

N

G

G

-

E

-

I

-

V

T

T

V

T

Y

F

C

P

P

K

P

P

I

V

G

G

H

H

I

Y

Q

Q

D

I

G

D

G

G

D

D

Y

Y

I

H

E

A

S

S

W

W

Q

Q

P

P

H

A

P

E

M

I

S

S

E

E

E

K

M

A

I

E

K

R

K

E

A

V

Q

Y

E

R

M

I

A

A

N

K

K

R

I

I

T

T

T

D

V

V

L

L

G

G

N

L

G

G

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I

F

F

G

G

V

V

E

E

L

M

F

F

I

V

R

K

G

G

D

D

E

K

V

V

I

W

F

A

S

N

E

E

V

V

S

S

P

P

R

R

P

P

H

H

D

D

T

T

G

G

M

M

V

V

T

T

M

L

I

A

T

S

Q

H

-

P

N

G

M

F

S

S

E

E

F

F

E

A

I

L

H

H

L

L

R

R

S

A

I

V

L

L

G

G

L

L

P

P

V

I

D

P

-

G

-

E

-

W

-

V

-

D

-

G

-

Y

-

R

-

L

I

F

Q

P

L

M

I

L

T

I

A

P

G

A

A

A

S

T

S

H

V

V

I

I

K

K

S

A

K

K

I

V

H

S

K

G

Y

Y

A

S

P

P

Q

R

Y

F

D

R

-

G

I

L

S

V

D

K

A

A

V

L

K

S

V

V

P

P

N

N

T

A

K

T

L

V

R

R

I

L

F

F

G

G

K

K

P

P

L

E

A

A

K

Y

V

V

G

G

R

R

M

L

G

G

V

I

A

A

L

L

A

A

S

W

A

D

N

K

T

D

V

V

E

E

E

V

A

A

R

K

K

R

N

K

A

A

K

E

K

M

C

V

A

A

N

H

G

M

V

I

I

E

I

L

R

R

R118 (= R112) binding ATP
K159 (= K153) binding ATP
EEHI 199:202 (≠ EEFI 195:198) binding ATP
E207 (= E203) binding ATP

2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
49% identity, 100% coverage: 2:390/390 of query aligns to 10:400/409 of 2dwcB

query
sites
2dwcB

L

L

G

G

T

T

P

A

L

T

F

T

S

D

N

S

G

A

T

Q

K

K

I

I

L

L

G

G

S

S

G

G

E

E

L

L

G

G

K

K

E

E

V

I

I

A

I

I

E

E

A

A

Q

Q

R

R

L

L

G

G

I

V

E

E

C

V

V

V

A

A

V

V

D

D

S

R

Y

Y

D

A

N

N

A

A

P

P

A

A

M

M

Q

Q

V

V

A

A

H

H

R

R

S

S

Y

Y

V

V

I

G

D

N

M

M

K

M

D

D

G

K

D

D

A

F

L

L

K

W

A

S

I

V

L

V

E

E

K

R

E

E

V

K

P

P

D

D

Y

A

I

I

P

P

E

E

I

I

E

E

A

A

I

I

N

N

T

L

D

D

I

A

L

L

I

F

E

E

M

F

E

E

E

K

F

D

G

G

H

Y

Q

F

V

V

P

P

T

N

A

A

N

R

A

A

A

T

K

W

I

I

T

A

M

M

D

H

R
|
R

E

E

G

R

I

L

R

R

R

E

L

T

A

L

S

V

E

K

Q

E

L

A

N

K

I

V

P

P

T

T

A

S

K

R

Y

Y

E

M

F

Y

A

A

E

T

S

T

L

L

E

D

E

E

L

L

K

Y

K

E

A

A

V

C

K

E

K

K

I

I

G

G

A

Y

P

P

C

C

I
x
H

V

T

K
|
K

P

A

I

I

M

M

S

S

S

-

G

-

K

-

G

G

Q

S

S

Y

T

F

V

V

K

K

S

G

E

P

K

-

D

-

E

E

D

D

I

I

E

P

Y

K

A

A

W

W

N

E

Y

-

A

-

Q

-

A

-

G

-

A

-

R

-

G

-

I

-

G

E

K

E

K

K

V

I

I

I

V

V

E

E

F
x
H

I
|
I

N

D

F
|
F

D

D

Y

V

E
|
E

I

V

T

T

L

E

L

L

T

A

A

V

R

R

-

H

-

F

T

D

A

E

H

N

G

G

-

E

-

I

-

V

T

T

V

T

Y

F

C

P

P

K

P

P

I

V

G

G

H

H

I

Y

Q

Q

D

I

G

D

G

G

D

D

Y

Y

I

H

E

A

S

S

W

W

Q

Q

P

P

H

A

P

E

M

I

S

S

E

E

E

K

M

A

I

E

K

R

K

E

A

V

Q

Y

E

R

M

I

A

A

N

K

K

R

I

I

T

T

T

D

V

V

L

L

G

G

N

L

G

G

I

I

F

F

G

G

V

V

E
|
E

L

M

F
|
F

I

V

R

K

G

G

D

D

E

K

V

V

I

W

F

A

S

N

E
|
E

V

V

S

S

P

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

M

M

V

V

T

T

M

L

I

A

T

S

Q

H

-

P

N

G

M

F

S

S

E

E

F

F

E

A

I

L

H

H

L

L

R

R

S

A

I

V

L

L

G

G

L

L

P

P

V

I

D

P

-

G

-

E

-

W

-

V

-

D

-

G

-

Y

-

R

-

L

I

F

Q

P

L

M

I

L

T

I

A

P

G

A

A

A

S

T

S

H

V

V

I

I

K

K

S

A

K

K

I

V

H

S

K

G

Y

Y

A

S

P

P

Q

R

Y

F

D

R

-

G

I

L

S

V

D

K

A

A

V

L

K

S

V

V

P

P

N

N

T

A

K

T

L

V

R

R

I

L

F

F

G

G

K

K

P

P

L

E

A

A

K

Y

V

V

G

G

R

R

R
|
R

M

L

G

G

V

I

A

A

L

L

A

A

S

W

A

D

N

K

T

D

V

V

E

E

E

V

A

A

R

K

K

R

N

K

A

A

K

E

K

M

C

V

A

A

N

H

G

M

V

I

I

E

I

L

R

R

active site: E265 (= E267), E277 (= E279), D284 (= D286), T285 (= T287), R372 (= R362)
binding adenosine-5'-diphosphate: R120 (= R112), H159 (≠ I151), K161 (= K153), H190 (≠ F197), I191 (= I198), F193 (= F200), E196 (= E203), F267 (= F269), E277 (= E279)

3k5iA Crystal structure of n5-carboxyaminoimidazole synthase from aspergillus clavatus in complex with adp and 5-aminoimadazole ribonucleotide (see paper)
28% identity, 89% coverage: 29:376/390 of query aligns to 23:367/381 of 3k5iA

query
sites
3k5iA

A

A

Q

N

R

R

L

L

G

N

I

I

E

Q

C

V

V

N

A

V

V

L

D

D

S

A

Y

-

D

D

N

N

A

S

P

P

A

A

M

K

Q

Q

V

I

-

S

A

A

H

H

R

D

S

G

Y

H

V

V

I

T

-

G

D

S

M

F

K

K

D

E

G

R

D

E

A

A

L

V

K

R

A

Q

I

L

L

-

E

-

K

A

E

K

V

T

P

C

D

D

Y

V

I

V

I

T

P

A

E
|
E

I
|
I

E

E

A

H

I

V

N

D

T

T

D

Y

I

A

L

L

I

E

E

E

M

V

E

-

F

-

G

-

H

-

Q

-

V

-

P

-

T

-

A

A

N

S

A

E

A

V

K

K

I

I

T

E

M

P

D

S

R

W

E

Q

G

A

I

I

R

R

R

T

L

I

A

Q

S

N

E
x
K

-

F

-

N

-

Q

-

K

-

E

-

H

-

L

-

R

Q

K

L

Y

N

G

I

I

P

P

T

M

A

A

K

E

Y

H

-

R

E

E

F

L

A

V

E

E

S

N

L

T

-

P

E

A

E

E

L

L

K

A

K

K

A

V

V

G

K

E

K

Q

I

L

G

G

A

Y

P

P

C

L

I

M

V

L

K
|
K

P

S

-

K

I

T

M

M

S

A

S
x
Y

S
x
D

G
|
G

K
x
R

G

G

Q

N

S

F

T

R

V

V

K

N

S

S

E

Q

K

-

D

-

E

D

D

D

I

I

E

P

Y

E

A

A

W

L

N

E

Y

A

A

L

Q

K

A

-

G

-

A

-

R

-

G

-

I

-

G

D

K

R

K

P

V

L

I

Y

V

A

E

E

E

K

F
x
W

I
x
A

N

Y

F
|
F

D

K

Y

M

E
|
E

I

L

T

A

L

V

L

I

T

V

A

V

R

K

T

T

A

K

H

D

G

E

T

V

V

L

Y

S

C

Y

P

P

P

T

I

V

G

E

H

T

I

V

Q
|
Q

D

E

G

D

G
x
S

D

I

Y

C

I

K

E

L

S

V

W

Y

Q

A

P

P

-

A

H

R

P

N

M

V

S

S

E

D

E

A

M

I

I

N

K

Q

K

K

A

A

Q

Q

E

E

M

L

A

A

N

R

K

K

I

A

T

V

T

A

V

A

L

F

G

D

G

G

N

K

G

G

I

V

F

F

G

G

V

V

E
|
E

L

M

F

F

I

L

-

L

R

E

G

D

D

D

E

S

V

I

I

M

F

L

S

C

E
|
E

V

I

S

A

P

S

R
|
R

P

I

H

H

D
x
N

T
x
S

G

G

M

H

V

Y

T

T

M

I

I

E

T

G

Q

C

N

A

M

L

S

S

E

Q

F

F

E

D

I

A

H

H

L

L

R

R

S

A

I

I

L

L

G

D

L

L

P

P

V

I

D

P

I

A

Q

Q

L

S

I

L

T

E

A

I

G

R

A

Q

S

P

S

S

V

I

I

M

K

L

S

N

K

I

I

I

H

G

K

G

Y

A

A

A

P

P

Q

D

Y

T

D

H

I

L

S

Q

D

A

-

A

-

E

-

C

A

A

V

L

K

S

V

I

P

P

N

N

T

A

K

S

L

I

R

H

I

L

F

Y

G

S

K
|
K

P

G

L

A

A

A

K

K

V

P

G

G

R
|
R

R
x
K

M

M

G

G

V

H

A

I

L

T

A

V

S

T

A

A

N

P

T

T

V

M

E

H

E

E

A

A

active site: E254 (= E267), E267 (= E279), N274 (≠ D286), S275 (≠ T287), K353 (≠ R362)
binding adenosine-5'-diphosphate: K104 (≠ E121), K146 (= K153), Y152 (≠ S158), D153 (≠ S159), G154 (= G160), W183 (≠ F197), A184 (≠ I198), F186 (= F200), E189 (= E203), Q211 (= Q225), S214 (≠ G228), E267 (= E279)
binding 5-aminoimidazole ribonucleotide: E73 (= E80), I74 (= I81), Y152 (≠ S158), D153 (≠ S159), R155 (≠ K161), R271 (= R283), K345 (= K354), R352 (= R361)
binding magnesium ion: E254 (= E267), E267 (= E279)

Sites not aligning to the query:

binding 5-aminoimidazole ribonucleotide: 13, 14, 15

3k5hA Crystal structure of carboxyaminoimidazole ribonucleotide synthase from asperigillus clavatus complexed with atp (see paper)
28% identity, 89% coverage: 29:376/390 of query aligns to 23:367/382 of 3k5hA

query
sites
3k5hA

A

A

Q

N

R

R

L

L

G

N

I

I

E

Q

C

V

V

N

A

V

V

L

D

D

S

A

Y

-

D

D

N

N

A

S

P

P

A

A

M

K

Q

Q

V

I

-

S

A

A

H

H

R

D

S

G

Y

H

V

V

I

T

-

G

D

S

M

F

K

K

D

E

G

R

D

E

A

A

L

V

K

R

A

Q

I

L

L

-

E

-

K

A

E

K

V

T

P

C

D

D

Y

V

I

V

I

T

P

A

E

E

I

I

E

E

A

H

I

V

N

D

T

T

D

Y

I

A

L

L

I

E

E

E

M

V

E

-

F

-

G

-

H

-

Q

-

V

-

P

-

T

-

A

A

N

S

A

E

A

V

K

K

I

I

T

E

M

P

D

S

R

W

E

Q

G

A

I

I

R

R

R

T

L

I

A

Q

S

N

E
x
K

-

F

-

N

-

Q

-

K

-

E

-

H

-

L

-

R

Q

K

L

Y

N

G

I

I

P

P

T

M

A

A

K

E

Y

H

-

R

E

E

F

L

A

V

E

E

S

N

L

T

-

P

E

A

E

E

L

L

K

A

K

K

A

V

V

G

K

E

K

Q

I

L

G

G

A

Y

P

P

C

L

I

M

V

L

K
|
K

P

S

-

K

I

T

M

M

S

A

S
x
Y

S
x
D

G
|
G

K

R

G

G

Q
x
N

S

F

T

R

V

V

K

N

S

S

E

Q

K

-

D

-

E

D

D

D

I

I

E

P

Y

E

A

A

W

L

N

E

Y

A

A

L

Q

K

A

-

G

-

A

-

R

-

G

-

I

-

G

D

K

R

K

P

V

L

I

Y

V

A

E
|
E

E

K

F
x
W

I
x
A

N

Y

F
|
F

D

K

Y

M

E
|
E

I

L

T

A

L

V

L

I

T

V

A

V

R

K

T

T

A

K

H

D

G

E

T

V

V

L

Y

S

C

Y

P

P

P

T

I

V

G

E

H

T

I

V

Q

Q

D

E

G

D

G

S

D

I

Y

C

I

K

E

L

S

V

W

Y

Q

A

P

P

-

A

H

R

P

N

M

V

S

S

E

D

E

A

M

I

I

N

K

Q

K

K

A

A

Q

Q

E

E

M

L

A

A

N

R

K

K

I

A

T

V

T

A

V

A

L

F

G

D

G

G

N

K

G

G

I

V

F

F

G

G

V

V

E
|
E

L

M

F
|
F

I

L

-

L

R

E

G

D

D

D

E

S

V

I

I

M

F

L

S

C

E
|
E

V

I

S

A

P

S

R

R

P

I

H

H

D
x
N

T
x
S

G

G

M

H

V

Y

T

T

M

I

I

E

T

G

Q

C

N

A

M

L

S

S

E

Q

F

F

E

D

I

A

H

H

L

L

R

R

S

A

I

I

L

L

G

D

L

L

P

P

V

I

D

P

I

A

Q

Q

L

S

I

L

T

E

A

I

G

R

A

Q

S

P

S

S

V

I

I

M

K

L

S

N

K

I

I

I

H

G

K

G

Y

A

A

A

P

P

Q

D

Y

T

D

H

I

L

S

Q

D

A

-

A

-

E

-

C

A

A

V

L

K

S

V

I

P

P

N

N

T

A

K

S

L

I

R

H

I

L

F

Y

G

S

K

K

P

G

L

A

A

A

K

K

V

P

G

G

R

R

R
x
K

M

M

G

G

V

H

A

I

L

T

A

V

S

T

A

A

N

P

T

T

V

M

E

H

E

E

A

A

active site: Y152 (≠ S158), G154 (= G160), E254 (= E267), E267 (= E279), N274 (≠ D286), S275 (≠ T287), K353 (≠ R362)
binding adenosine-5'-triphosphate: K104 (≠ E121), K146 (= K153), Y152 (≠ S158), D153 (≠ S159), G154 (= G160), N157 (≠ Q163), E181 (= E195), W183 (≠ F197), A184 (≠ I198), F186 (= F200), E189 (= E203), E254 (= E267), F256 (= F269), E267 (= E279)
binding magnesium ion: E254 (= E267), E267 (= E279)

3r5hA Crystal structure of adp-air complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 95% coverage: 11:381/390 of query aligns to 2:367/383 of 3r5hA

query
sites
3r5hA

T

T

K

R

I

I

L

I

L

L

L

P

G

G

S

K

-

T

-

I

-

G

-

I

-

G

G
|
G

E
x
Q

L
|
L

G

G

K

R

E

M

V

M

I

A

I

L

E

A

A

A

Q

K

R

E

L

M

G

G

I

Y

E

K

C

I

V

A

A

V

V

L

D

D

S

P

Y

T

D

K

N

N

A

S

P

P

A

C

M

A

Q

Q

V

V

A

A

H

D

R

I

S

E

Y

I

V

V

I

A

D

S

M

Y

K

D

D

D

G

L

D

K

A

A

L

I

K

Q

A

H

I

L

L

-

E

-

K

A

E

E

V

I

P

S

D

D

Y

V

I

V

I

T

P

Y

E
|
E

I

F

E

E

A

N

I

I

N

D

T

Y

D

R

I

C

L

L

I

Q

E

W

M

L

E

E

E

K

F

-

G

-

H

H

Q

A

V

Y

V

L

P

P

T

Q

A

G

N

S

A

Q

A

L

K

L

I

S

T

K

M

T

D

Q

R

N

E
x
R

G

F

I

T

R

E

R

K

L

N

A

A

S

I

E

E

Q

K

L

A

N

G

I

L

P

P

T

V

A

A

K

T

Y

Y

E

R

F

L

A

V

E

Q

S

N

L

Q

E

E

E

Q

L

L

K

T

K

E

A

A

V

I

K

A

K

E

I

L

G

S

A

Y

P

P

C

S

I

V

V

L

K
|
K

P

T

I

T

M

T

S

G

-
x
Y

S

D

G

G

K

K

G

G

Q
|
Q

S

V

T

V

V

L

K

R

S

S

E

E

K

A

D

D

E

V

D

D

I

-

E

-

Y

-

A

-

W

-

N

-

Y

-

A

-

Q

E

A

A

G

R

A

K

R

L

G

A

I

N

G

A

K

A

K

E

V

C

I

I

V

L

E

E

E

K

F
x
W

I
x
V

N

P

F
|
F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

A

I

R

R

T

S

A

V

H

S

G

G

T

E

V

T

Y

K

C

V

P

F

P

P

I

V

G

A

-

E

H

N

I

I

Q

H

D

V

G

N

G
x
N

D

I

Y

L

I

H

E

E

S

S

W

I

Q

V

P

P

H

A

P

R

M

I

S

T

E

E

M

L

I

S

K

Q

K

K

A

A

Q

I

E

A

M

Y

A

A

N

K

K

V

I

L

T

A

T

D

V

E

L

L

G

E

G

L

N

V

G

G

I

T

F

L

G

A

V

V

E

E

L

M

F
|
F

I

A

R

T

G

A

D

D

-

G

E

E

V

I

I

Y

F

I

S
x
N

E
|
E

V

L

S

A

P

P

R
|
R

P

P

H

H

D

N

T

S

G

G

M

H

V

Y

T

T

M

Q

I

D

T

A

Q

C

N

E

M

T

S

S

E

Q

F

F

E

G

I

Q

H

H

L

I

R

R

S

A

I

I

L

C

G

N

L

L

P

P

V

L

D

-

I

-

Q

-

L

-

I

-

T

-

A

-

G

G

A

E

S

T

S

N

V

L

I

L

K

K

S

P

K

V

I

V

H

M

K

V

Y

N

A

I

P

L

Q

G

Y

E

D

H

I

I

S

E

D

G

A

V

V

L

K

R

V

Q

P

V

N

N

-

R

-

L

-

T

-

G

T

C

K

Y

L

L

R

H

I

L

F

Y

G

G

K
|
K

P

E

L

E

A

A

K

K

V

A

G

Q

R
|
R

R

K

M

M

G

G

V

H

A

V

L

N

A

I

S

L

A

N

N

D

T

N

V

I

E

E

E

V

A

A

R

L

K

E

N

K

A

A

K

K

binding adenosine-5'-diphosphate: R107 (≠ E113), K147 (= K153), Q158 (= Q163), W184 (≠ F197), V185 (≠ I198), F187 (= F200), E190 (= E203), N216 (≠ G228), F257 (= F269), N267 (≠ S278), E268 (= E279)
binding 5-aminoimidazole ribonucleotide: G17 (= G19), Q18 (≠ E20), L19 (= L21), E76 (= E80), Y153 (vs. gap), R272 (= R283), K340 (= K354), R347 (= R361)

3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 95% coverage: 11:381/390 of query aligns to 1:366/380 of 3v4sA

query
sites
3v4sA

T

T

K

R

I

I

L

I

L

L

L

P

G

G

S

K

-

T

-

I

-

G

-

I

-

G

G

G

E

Q

L

L

G

G

K

R

E

M

V

M

I

A

I

L

E

A

A

A

Q

K

R

E

L

M

G

G

I

Y

E

K

C

I

V

A

A

V

V

L

D

D

S

P

Y

T

D

K

N

N

A

S

P

P

A

C

M

A

Q

Q

V

V

A

A

H

D

R

I

S

E

Y

I

V

V

I

A

D

S

M

Y

K

D

D

D

G

L

D

K

A

A

L

I

K

Q

A

H

I

L

L

-

E

-

K

A

E

E

V

I

P

S

D

D

Y

V

I

V

I

T

P

Y

E

E

I

F

E

E

A

N

I

I

N

D

T

Y

D

R

I

C

L

L

I

Q

E

W

M

L

E

E

E

K

F

-

G

-

H

H

Q

A

V

Y

V

L

P

P

T

Q

A

G

N

S

A

Q

A

L

K

L

I

S

T

K

M

T

D

Q

R

N

E
x
R

G

F

I

T

R

E

R

K

L

N

A

A

S

I

E

E

Q

K

L

A

N

G

I

L

P

P

T

V

A

A

K

T

Y

Y

E

R

F

L

A

V

E

Q

S

N

L

Q

E

E

E

Q

L

L

K

T

K

E

A

A

V

I

K

A

K

E

I

L

G

S

A

Y

P

P

C

S

I

V

V

L

K
|
K

P

T

I

T

M

T

S

G

-
x
Y

S

D

G
|
G

K

K

G

G

Q
|
Q

S

V

T

V

V

L

K

R

S

S

E

E

K

A

D

D

E

V

D

D

I

-

E

-

Y

-

A

-

W

-

N

-

Y

-

A

-

Q

E

A

A

G

R

A

K

R

L

G

A

I

N

G

A

K

A

K

E

V

C

I

I

V

L

E

E

E

K

F
x
W

I
x
V

N

P

F

F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

A

I

R

R

T

S

A

V

H

S

G

G

T

E

V

T

Y

K

C

V

P

F

P

P

I

V

G

A

-

E

H

N

I

I

Q

H

D

V

G

N

G
x
N

D

I

Y

L

I

H

E

E

S

S

W

I

Q

V

P

P

H

A

P

R

M

I

S

T

E

E

M

L

I

S

K

Q

K

K

A

A

Q

I

E

A

M

Y

A

A

N

K

K

V

I

L

T

A

T

D

V

E

L

L

G

E

G

L

N

V

G

G

I

T

F

L

G

A

V

V

E

E

L

M

F
|
F

I

A

R

T

G

A

D

D

-

G

E

E

V

I

I

Y

F

I

S
x
N

E
|
E

V

L

S

A

P

P

R
|
R

P

P

H
|
H

D
x
N

T

S

G

G

M

H

V

Y

T

T

M

Q

I

D

T

A

Q

C

N

E

M

T

S

S

E

Q

F

F

E

G

I

Q

H

H

L

I

R

R

S

A

I

I

L

C

G

N

L

L

P

P

V

L

D

-

I

-

Q

-

L

-

I

-

T

-

A

-

G

G

A

E

S

T

S

N

V

L

I

L

K

K

S

P

K

V

I

V

H

M

K

V

Y

N

A

I

P

L

Q

G

Y

E

D

H

I

I

S

E

D

G

A

V

V

L

K

R

V

Q

P

V

N

N

-

R

-

L

-

T

-

G

T

C

K

Y

L

L

R

H

I

L

F

Y

G

G

K

K

P

E

L

E

A

A

K

K

V

A

G

Q

R

R

R

K

M

M

G

G

V

H

A

V

L

N

A

I

S

L

A

N

N

D

T

N

V

I

E

E

E

V

A

A

R

L

K

E

N

K

A

A

K

K

binding adenosine-5'-diphosphate: R106 (≠ E113), K146 (= K153), Y152 (vs. gap), G154 (= G160), Q157 (= Q163), W183 (≠ F197), V184 (≠ I198), E189 (= E203), N215 (≠ G228), F256 (= F269), N266 (≠ S278), E267 (= E279)
binding carbonate ion: R271 (= R283), H273 (= H285), N274 (≠ D286)

4dlkA Crystal structure of atp-ca++ complex of purk: n5- carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 95% coverage: 11:381/390 of query aligns to 2:367/380 of 4dlkA

query
sites
4dlkA

T

T

K

R

I

I

L

I

L

L

L

P

G

G

S

K

-

T

-

I

-

G

-

I

-

G

G

G

E

Q

L

L

G

G

K

R

E

M

V

M

I

A

I

L

E

A

A

A

Q

K

R

E

L

M

G

G

I

Y

E

K

C

I

V

A

A

V

V

L

D

D

S

P

Y

T

D

K

N

N

A

S

P

P

A

C

M

A

Q
|
Q

V

V

A
|
A

H

D

R

I

S

E

Y

I

V

V

I

A

D

S

M

Y

K

D

D

D

G

L

D

K

A

A

L

I

K

Q

A

H

I

L

L

-

E

-

K

A

E

E

V

I

P

S

D

D

Y

V

I

V

I

T

P

Y

E
|
E

I
x
F

E

E

A

N

I

I

N

D

T

Y

D

R

I

C

L

L

I

Q

E

W

M

L

E

E

E

K

F

-

G

-

H

H

Q

A

V

Y

V

L

P

P

T

Q

A

G

N

S

A

Q

A

L

K

L

I

S

T

K

M

T

D

Q

R

N

E
x
R

G

F

I

T

R

E

R

K

L

N

A

A

S

I

E

E

Q

K

L

A

N

G

I

L

P

P

T

V

A

A

K

T

Y

Y

E

R

F

L

A

V

E

Q

S

N

L

Q

E

E

E

Q

L

L

K

T

K

E

A

A

V

I

K

A

K

E

I

L

G

S

A

Y

P

P

C

S

I

V

V

L

K
|
K

P

T

I

T

M

T

S

G

-
x
Y

S
x
D

G
|
G

K

K

G

G

Q
|
Q

S

V

T

V

V

L

K

R

S

S

E

E

K

A

D

D

E

V

D

D

I

-

E

-

Y

-

A

-

W

-

N

-

Y

-

A

-

Q

E

A

A

G

R

A

K

R

L

G

A

I

N

G

A

K

A

K

E

V

C

I

I

V

L

E

E

E

K

F
x
W

I
x
V

N

P

F
|
F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

A

I

R

R

T

S

A

V

H

S

G

G

T

E

V

T

Y

K

C

V

P

F

P

P

I

V

G

A

-

E

H

N

I

I

Q

H

D

V

G

N

D

I

Y

L

I

H

E

E

S

S

W

I

Q

V

P

P

H

A

P

R

M

I

S

T

E

E

M

L

I

S

K

Q

K

K

A

A

Q

I

E

A

M

Y

A

A

N

K

K

V

I

L

T

A

T

D

V

E

L

L

G

E

G

L

N

V

G

G

I

T

F

L

G

A

V

V

E
|
E

L

M

F
|
F

I

A

R

T

G

A

D

D

-

G

E

E

V

I

I

Y

F

I

S
x
N

E
|
E

V

L

S

A

P

P

R
|
R

P

P

H
|
H

D
x
N

T
x
S

G

G

M

H

V

Y

T

T

M

Q

I

D

T

A

Q

C

N

E

M

T

S

S

E

Q

F

F

E

G

I

Q

H

H

L

I

R

R

S

A

I

I

L

C

G

N

L

L

P

P

V

L

D

-

I

-

Q

-

L

-

I

-

T

-

A

-

G

G

A

E

S

T

S

N

V

L

I

L

K

K

S

P

K

V

I

V

H

M

K

V

Y

N

A

I

P

L

Q

G

Y

E

D

H

I

I

S

E

D

G

A

V

V

L

K

R

V

Q

P

V

N

N

-

R

-

L

-

T

-

G

T

C

K

Y

L

L

R

H

I

L

F

Y

G

G

K
|
K

P

E

L

E

A

A

K

K

V

A

G

Q

R
|
R

R
x
K

M

M

G

G

V

H

A

V

L

N

A

I

S

L

A

N

N

D

T

N

V

I

E

E

E

V

A

A

R

L

K

E

N

K

A

A

K

K

active site: Y153 (vs. gap), G155 (= G160), E255 (= E267), E268 (= E279), N275 (≠ D286), S276 (≠ T287), K348 (≠ R362)
binding adenosine-5'-triphosphate: E76 (= E80), F77 (≠ I81), R107 (≠ E113), K147 (= K153), Y153 (vs. gap), D154 (≠ S159), G155 (= G160), Q158 (= Q163), W184 (≠ F197), V185 (≠ I198), F187 (= F200), E190 (= E203), E255 (= E267), F257 (= F269), N267 (≠ S278), E268 (= E279), R272 (= R283), H274 (= H285), N275 (≠ D286), K340 (= K354), R347 (= R361), K348 (≠ R362)
binding calcium ion: E255 (= E267), E268 (= E279)
binding phosphate ion: Q47 (= Q49), A49 (= A51)

3v4sB Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 95% coverage: 11:381/390 of query aligns to 3:368/381 of 3v4sB

query
sites
3v4sB

T

T

K

R

I

I

L

I

L

L

L

P

G

G

S

K

-

T

-

I

-

G

-

I

-

G

G

G

E

Q

L

L

G

G

K

R

E

M

V

M

I

A

I

L

E

A

A

A

Q

K

R

E

L

M

G

G

I

Y

E

K

C

I

V

A

A

V

V

L

D

D

S

P

Y

T

D

K

N

N

A

S

P

P

A

C

M

A

Q

Q

V

V

A

A

H

D

R

I

S

E

Y

I

V

V

I

A

D

S

M

Y

K

D

D

D

G

L

D

K

A

A

L

I

K

Q

A

H

I

L

L

-

E

-

K

A

E

E

V

I

P

S

D

D

Y

V

I

V

I

T

P

Y

E

E

I

F

E

E

A

N

I

I

N

D

T

Y

D

R

I

C

L

L

I

Q

E

W

M

L

E

E

E

K

F

-

G

-

H

H

Q

A

V

Y

V

L

P

P

T

Q

A

G

N

S

A

Q

A

L

K

L

I

S

T

K

M
x
T

D

Q

R

N

E
x
R

G

F

I

T

R
x
E

R

K

L

N

A

A

S

I

E

E

Q

K

L

A

N

G

I

L

P

P

T

V

A

A

K

T

Y

Y

E

R

F

L

A

V

E

Q

S

N

L

Q

E

E

E

Q

L

L

K

T

K

E

A

A

V

I

K

A

K

E

I

L

G

S

A

Y

P

P

C

S

I

V

V

L

K
|
K

P

T

I

T

M

T

S

G

-
x
Y

S
x
D

G
|
G

K

K

G

G

Q
|
Q

S

V

T

V

V

L

K

R

S

S

E

E

K

A

D

D

E

V

D

D

I

-

E

-

Y

-

A

-

W

-

N

-

Y

-

A

-

Q

E

A

A

G

R

A

K

R

L

G

A

I

N

G

A

K

A

K

E

V

C

I

I

V

L

E
|
E

E

K

F
x
W

I
x
V

N

P

F
|
F

D

E

Y

K

E
|
E

I

V

T

S

L

V

L

I

T

V

A

I

R

R

T

S

A

V

H

S

G

G

T

E

V

T

Y

K

C

V

P

F

P

P

I

V

G

A

-

E

H

N

I

I

Q
x
H

D

V

G

N

G
x
N

D

I

Y

L

I

H

E

E

S

S

W

I

Q

V

P

P

H

A

P

R

M

I

S

T

E

E

M

L

I

S

K

Q

K

K

A

A

Q

I

E

A

M

Y

A

A

N

K

K

V

I

L

T

A

T

D

V

E

L

L

G

E

G

L

N

V

G

G

I

T

F

L

G

A

V

V

E
|
E

L

M

F
|
F

I

A

R

T

G

A

D

D

-

G

E

E

V

I

I

Y

F

I

S

N

E
|
E

V
x
L

S

A

P

P

R
|
R

P

P

H
|
H

D
x
N

T

S

G

G

M

H

V

Y

T

T

M

Q

I

D

T

A

Q

C

N

E

M

T

S

S

E

Q

F

F

E

G

I

Q

H

H

L

I

R

R

S

A

I

I

L

C

G

N

L

L

P

P

V

L

D

-

I

-

Q

-

L

-

I

-

T

-

A

-

G

G

A

E

S

T

S

N

V

L

I

L

K

K

S

P

K

V

I

V

H

M

K

V

Y

N

A

I

P

L

Q

G

Y

E

D

H

I

I

S

E

D

G

A

V

V

L

K

R

V

Q

P

V

N

N

-

R

-

L

-

T

-

G

T

C

K

Y

L

L

R

H

I

L

F

Y

G

G

K

K

P

E

L

E

A

A

K

K

V

A

G

Q

R

R

R

K

M

M

G

G

V

H

A

V

L

N

A

I

S

L

A

N

N

D

T

N

V

I

E

E

E

V

A

A

R

L

K

E

N

K

A

A

K

K

binding adenosine-5'-triphosphate: R108 (≠ E113), K148 (= K153), Y154 (vs. gap), D155 (≠ S159), G156 (= G160), Q159 (= Q163), E183 (= E195), W185 (≠ F197), V186 (≠ I198), F188 (= F200), E191 (= E203), H214 (≠ Q225), N217 (≠ G228), E256 (= E267), F258 (= F269), E269 (= E279)
binding carbonate ion: R273 (= R283), H275 (= H285), N276 (≠ D286)
binding magnesium ion: T105 (≠ M110), E111 (≠ R116), E256 (= E267), E269 (= E279), L270 (≠ V280)

3q2oB Crystal structure of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
26% identity, 95% coverage: 11:381/390 of query aligns to 2:367/377 of 3q2oB

query
sites
3q2oB

T

T

K

R

I

I

L

I

L

L

L

P

G

G

S

K

-

T

-

I

-

G

-

I

-

G

G

G

E

Q

L

L

G

G

K

R

E

M

V

M

I

A

I

L

E

A

A

A

Q

K

R

E

L

M

G

G

I

Y

E

K

C

I

V

A

A

V

V

L

D

D

S

P

Y

T

D

K

N

N

A

S

P

P

A

C

M

A

Q

Q

V

V

A

A

H

D

R

I

S

E

Y

I

V

V

I

A

D

S

M

Y

K

D

D

D

G

L

D

K

A

A

L

I

K

Q

A

H

I

L

L

-

E

-

K

A

E

E

V

I

P

S

D

D

Y

V

I

V

I

T

P

Y

E

E

I

F

E

E

A

N

I

I

N

D

T

Y

D

R

I

C

L

L

I

Q

E

W

M

L

E

E

E

K

F

-

G

-

H

H

Q

A

V

Y

V

L

P

P

T

Q

A

G

N

S

A

Q

A

L

K

L

I

S

T

K

M
x
T

D

Q

R

N

E

R

G

F

I

T

R
x
E

R

K

L

N

A

A

S

I

E

E

Q

K

L

A

N

G

I

L

P

P

T

V

A

A

K

T

Y

Y

E

R

F

L

A

V

E

Q

S

N

L

Q

E

E

E

Q

L

L

K

T

K

E

A

A

V

I

K

A

K

E

I

L

G

S

A

Y

P

P

C

S

I

V

V

L

K

K

P

T

I

T

M

T

S

G

-

Y

S

D

G

G

K

K

G

G

Q

Q

S

V

T

V

V

L

K

R

S

S

E

E

K

A

D

D

E

V

D

D

I

-

E

-

Y

-

A

-

W

-

N

-

Y

-

A

-

Q

E

A

A

G

R

A

K

R

L

G

A

I

N

G

A

K

A

K

E

V

C

I

I

V

L

E

E

E

K

F

W

I

V

N

P

F

F

D

E

Y

K

E

E

I

V

T

S

L

V

L

I

T

V

A

I

R

R

T

S

A

V

H

S

G

G

T

E

V

T

Y

K

C

V

P

F

P

P

I

V

G

A

-

E

H

N

I

I

Q

H

D

V

G

N

D

I

Y

L

I

H

E

E

S

S

W

I

Q

V

P

P

H

A

P

R

M

I

S

T

E

E

M

L

I

S

K

Q

K

K

A

A

Q

I

E

A

M

Y

A

A

N

K

K

V

I

L

T

A

T

D

V

E

L

L

G

E

G

L

N

V

G

G

I

T

F

L

G

A

V

V

E

E

L

M

F

F

I

A

R

T

G

A

D

D

-

G

E

E

V

I

I

Y

F

I

S

N

E

E

V
x
L

S

A

P

P

R

R

P

P

H

H

D

N

T

S

G

G

M

H

V

Y

T

T

M

Q

I

D

T

A

Q

C

N

E

M

T

S

S

E

Q

F

F

E

G

I

Q

H

H

L

I

R

R

S

A

I

I

L

C

G

N

L

L

P

P

V

L

D

-

I

-

Q

-

L

-

I

-

T

-

A

-

G

G

A

E

S

T

S

N

V

L

I

L

K

K

S

P

K

V

I

V

H

M

K

V

Y

N

A

I

P

L

Q

G

Y

E

D

H

I

I

S

E

D

G

A

V

V

L

K

R

V

Q

P

V

N

N

-

R

-

L

-

T

-

G

T

C

K

Y

L

L

R

H

I

L

F

Y

G

G

K

K

P

E

L

E

A

A

K

K

V

A

G

Q

R

R

R

K

M

M

G

G

V

H

A

V

L

N

A

I

S

L

A

N

N

D

T

N

V

I

E

E

E

V

A

A

R

L

K

E

N

K

A

A

K

K

binding magnesium ion: T104 (≠ M110), E110 (≠ R116), L269 (≠ V280)

3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
25% identity, 92% coverage: 27:383/390 of query aligns to 18:351/360 of 3ax6A

query
sites
3ax6A

I

L

E

E

A

A

Q

K

R

K

L

M

G

G

I

F

E

Y

C

V

V

I

A

V

V

L

D

D

S

P

Y

T

D

P

N

R

A

S

P

P

A

A

M

G

Q

Q

V

V

A

A

H

D

R

E

S

Q

Y

I

V

V

I

A

D

G

M

F

K

F

D

D

G

S

D

E

A

R

L

I

K

E

A

D

I

L

L

V

E

K

K

G

E

S

V

-

P

-

D

D

Y

V

I

T

P

Y

E

D

I

L

E

E

A

H

I

I

N

D

T

V

D

Q

I

T

L

L

I

K

E

K

M

L

E

Y

E

N

F

E

G

G

H

Y

Q

K

V

I

V

H

P

P

T

S

A

P

N

Y

A

T

A

L

K

E

I

I

T

I

M

Q

D

D

R
x
K

E

-

G

F

I

V

R

Q

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K

L

E

A

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S

L

E

K

Q

K

L

N

N

G

I

I

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P

T

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A

P

K

E

Y

Y

E

K

F

L

A

V

E

K

S

D

L

L

E

E

E

-

L

-

K

-

K

S

A

D

V

V

K

R

K

E

I

F

G

G

A

F

P

P

C

V

I
x
V

V

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K
|
K

P

-

I

-

M

-

S

-

S

A

S

R

G

K

K

G

G

G

Q

V

S

F

T

I

V

I

K

K

S

N

E

E

K

K

D

D

E

L

D

E

I

-

E

-

Y

-

A

-

W

-

N

-

Y

-

A

-

Q

-

A

-

G

-

A

-

R

N

G

A

I

I

G

K

K

G

K

E

V

T

I

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V

L

E
|
E

E

E

F
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F

I
x
V

N

E

F

I

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Y

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E
|
E

I

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L

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L

M

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A

A

R

R

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N

A

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I

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A

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Y

P

P

I

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G

V

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E

I

M

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Y

D

D

G

T

G

-

D

-

Y

-

I

-

E

-

S

V

W

I

Q

A

P

P

H

A

P

R

M

I

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E

E

E

E

K

M

Y

I

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K

K

K

I

A

A

Q

R

E

E

M

I

A

A

N

T

K

S

I

V

T

V

T

E

V

A

L

L

G

E

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G

N

V

G

G

I

I

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F

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I

E
|
E

L

M

F
|
F

I

L

-

T

R

K

G

Q

D

G

E

E

V

I

I

L

F

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S
x
N

E
|
E

V

I

S

A

P

P

R

R

P

P

H

H

D
x
N

T
x
S

G

G

M

H

V

Y

T

T

M

I

I

E

T

A

Q

C

N

V

M

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Q

F

F

E

E

I

Q

H

H

L

I

R

R

S

A

I

I

L

M

G

N

L

L

P

P

V

L

D

G

I

S

Q

T

-

E

-

L

L

L

I

I

T

P

A

A

G

V

A

M

S

V

S

N

V

L

I

L

K

G

S

E

K

E

I

G

H

Y

K

Y

Y

G

A

K

P

P

Q

A

Y

L

-

I

D

G

I

L

S

E

D

E

A

A

V

L

K

A

V

I

P

E

N

G

T

L

K

S

L

L

R

H

I

F

F

Y

G

G

K

K

P

K

L

E

A

T

K

R

V

P

G

Y

R

R

R
x
K

M

M

G

G

V

H

A

F

L

T

A

V

S

V

A

D

N

R

T

D

V

V

E

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A

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K

E

N

K

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A

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A

active site: E231 (= E267), E244 (= E279), N251 (≠ D286), S252 (≠ T287), K330 (≠ R362)
binding adenosine-5'-diphosphate: K101 (≠ R112), V136 (≠ I151), K138 (= K153), E164 (= E195), F166 (= F197), V167 (≠ I198), E172 (= E203), F233 (= F269), N243 (≠ S278)

3aw8A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermus thermophilus hb8
31% identity, 68% coverage: 99:364/390 of query aligns to 84:331/360 of 3aw8A

query
sites
3aw8A

V

L

V

Y

P

P

T

P

A

A

N

K

A

A

A

L

K

E

I

V

T

A

M

Q

D

D

R

R

E

-

G

-

I

L

R

R

R

E

L

K

A

T

S

F

E

F

Q

Q

-

G

L

L

N

G

I

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P

T

T

A

P

K

P

Y

F

E

H

F

P

A

V

E

D

S

G

L

P

E

E

E

D

L

L

K

E

A

G

V

L

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K

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G

A

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A

I
x
L

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|
K

P

-

I

-

M

-

S

-

S

T

G

R

K

R

G

G

Q
|
Q

S

A

T

L

V

V

K

R

S

T

E

E

K

-

D

E

E

E

D

A

I

L

E

E

Y

-

A

-

W

-

N

A

Y

L

A

K

Q

A

A

L

G

G

A

G

R

R

G

G

I

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G

-

K

-

V

-

I

I

V

L

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E

E

G

F
|
F

I
x
V

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P

F

F

D

D

Y

R

E
|
E

I

V

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S

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L

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A

A

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R

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G

A

R

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G

T

E

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V

-

A

Y

F

C

Y

P

P

P

L

I

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G

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H

N

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x
H

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D

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P

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M

A

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E

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A

M

L

I

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K

A

A

Q

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A

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A

A

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K

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I

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M

T

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V

A

L

L

G

D

G

Y

N

V

G

G

I

V

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L

G

A

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E

L

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F
|
F

I

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R

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D

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E

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I

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S

N

E
|
E

V

M

S

A

P

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R

R

P

V

H

H

D
x
N

T
x
S

G

G

M

H

V

W

T

T

M

I

I

E

T

G

Q

A

N

E

M

T

S

S

E

Q

F

F

E

E

I

N

H

H

L

L

R

R

S

A

I

V

L

L

G

G

L

L

P

P

V

L

D

G

I

-

Q

S

L

T

I

A

T

P

A

R

G

G

A

Q

S

S

S

A

V

M

I

V

K

-

S

N

K

L

I

I

H

G

K

E

Y

K

A

P

P

P

Q

-

Y

-

D

-

I

F

S

A

D

E

A

V

V

L

K

K

V

V

P

E

N

G

T

A

K

H

L

L

R

H

I

W

F

Y

G

G

K

K

P

A

L

V

A

-

K

R

V

P

G

G

R

R

R
x
K

M

V

G

G

active site: E240 (= E267), E252 (= E279), N259 (≠ D286), S260 (≠ T287), K329 (≠ R362)
binding adenosine monophosphate: L135 (≠ I151), K137 (= K153), Q142 (= Q163), F168 (= F197), V169 (≠ I198), E174 (= E203), H197 (≠ Q225), F242 (= F269), E252 (= E279)

4ma0A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with partially hydrolysed atp
28% identity, 77% coverage: 12:310/390 of query aligns to 2:290/366 of 4ma0A

query
sites
4ma0A

K

K

I

I

L

G

L

I

G

G

S

A

G

G

E

Q

L

L

G

A

K

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I

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A

A

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L

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G

G

I

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C

F

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H

A

C

V

L

D

G

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K

Y

N

D

G

N

D

A

C

P

A

A

E

M

E

Q

V

V

V

A

-

H

-

R

-

S

K

Y

T

V

V

I

T

D

D

M

I

K

E

D

L

G

T

D

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A

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D

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F

E

E

I

N

E

E

A

N

I

I

N

S

T

H

D

E

I

L

L

I

I

-

E

-

M

-

E

K

E

A

F

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G

N

H

H

Q

E

V

V

-

S

-

V

V

Y

P

P

T

S

A

A

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K

A

A

A

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K

A

I

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-

I

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G

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A

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T

A

A

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E

A

E

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Q

K

S

A

A

V

V

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D

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H

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G

A

L

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P

C

A

I
|
I

V

L

K
|
K

P

T

I

R

-

R

M

F

S

G

S
x
Y

S

D

G
|
G

K

K

G

G

Q

Q

S

F

T

V

V

I

K

R

S

S

E

Q

K

-

D

-

E

E

D

D

I

I

E

T

Y

K

A

A

W

W

N

D

Y

V

A

L

Q

K

A

D

G

A

A

P

R

D

G

G

I

L

G

-

K

-

V

-

I

I

V

Y

E
|
E

E
x
A

F
|
F

I
x
V

N

D

F

F

D

D

Y

Y

E
|
E

I

V

T

S

L

Q

L

I

T

C

A

T

R

A

T

D

A

L

H

K

G

G

T

N

V

I

Y

A

C

F

P

Y

P

P

I

L

G

A

-

R

H

N

I

T

Q
x
H

D

K

G

Q

G

G

D

I

Y

I

I

V

E

E

S

S

W

E

Q

A

P

P

H

F

P

E

M

-

S

N

E

V

M

L

I

A

K

E

K

K

A

A

Q

Q

E

Q

M

I

A

A

N

K

K

I

I

L

T

V

T

K

V

E

L

F

G

A

G

Y

N

V

G

G

I

T

F

L

G

A

V

I

E
|
E

L

F

F
|
F

I

V

R

K

G

G

D

D

E

E

V

L

I

I

F

V

S

N

E
|
E

V

I

S

A

P

P

R

R

P

V

H

H

D
x
N

T
x
S

G

G

M

H

V

W

T

S

M

I

I

D

T

G

Q

A

N

V

M

T

S

S

E

Q

F

F

E

E

I

N

H

H

L

V

R

R

S

A

I

I

L

A

G

G

L

L

active site: Y144 (≠ S158), G146 (= G160), E247 (= E267), E259 (= E279), N266 (≠ D286), S267 (≠ T287)
binding adenosine monophosphate: I136 (= I151), K138 (= K153), E175 (= E195), A176 (≠ E196), F177 (= F197), V178 (≠ I198), E183 (= E203), H206 (≠ Q225), F249 (= F269), E259 (= E279)

Sites not aligning to the query:

active site: 335

5jqwA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with adp
28% identity, 77% coverage: 12:310/390 of query aligns to 2:290/365 of 5jqwA

query
sites
5jqwA

K

K

I

I

L

G

L

I

G

G

S

A

G

G

E

Q

L

L

G

A

K

R

E

M

V

L

I

S

I

L

E

A

A

G

Q

T

R

P

L

L

G

G

I

L

E

E

C

F

V

H

A

C

V

L

D

G

S

K

Y

N

D

G

N

D

A

C

P

A

A

E

M

E

Q

V

V

V

A

-

H

-

R

-

S

K

Y

T

V

V

I

T

D

D

M

I

K

E

D

L

G

T

D

K

A

V

L

N

K

D

A

V

I

V

L

A

E

W

K

A

E

K

V

Q

P

F

D

D

Y

V

I

I

I

T

P

F

E

E

I

N

E

E

A

N

I

I

N

S

T

H

D

E

I

L

L

I

I

-

E

-

M

-

E

K

E

A

F

I

G

N

H

H

Q

E

V

V

-

S

-

V

V

Y

P

P

T

S

A

A

N

K

A

A

A

I

K

A

I

I

T

S

M

Q

D

D

R
|
R

E

L

G

-

I

L

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active site: Y144 (≠ S158), G146 (= G160), E247 (= E267), E259 (= E279), N266 (≠ D286), S267 (≠ T287)
binding adenosine-5'-diphosphate: R98 (= R112), K138 (= K153), G143 (≠ S157), Y144 (≠ S158), D145 (≠ S159), G146 (= G160), V178 (≠ I198), E183 (= E203), H206 (≠ Q225), F249 (= F269), E259 (= E279)

Sites not aligning to the query:

active site: 335

Query Sequence

>WP_198002428.1 NCBI__GCF_000017185.1:WP_198002428.1
MLGTPLFSNGTKILLLGSGELGKEVIIEAQRLGIECVAVDSYDNAPAMQVAHRSYVIDMK
DGDALKAILEKEVPDYIIPEIEAINTDILIEMEEFGHQVVPTANAAKITMDREGIRRLAS
EQLNIPTAKYEFAESLEELKKAVKKIGAPCIVKPIMSSSGKGQSTVKSEKDEDIEYAWNY
AQAGARGIGKKVIVEEFINFDYEITLLTARTAHGTVYCPPIGHIQDGGDYIESWQPHPMS
EEMIKKAQEMANKITTVLGGNGIFGVELFIRGDEVIFSEVSPRPHDTGMVTMITQNMSEF
EIHLRSILGLPVDIQLITAGASSVIKSKIHKYAPQYDISDAVKVPNTKLRIFGKPLAKVG
RRMGVALASANTVEEARKNAKKCANGVIIR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory