SitesBLAST
Comparing WP_200770547.1 NCBI__GCF_900141795.1:WP_200770547.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
38% identity, 92% coverage: 30:416/422 of query aligns to 45:412/412 of 7awmA
- binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ G73), G89 (= G75), G92 (= G78), A95 (≠ T81), V96 (≠ L82), Y99 (= Y85), M163 (≠ L162), F167 (≠ Y166), F293 (= F300), V297 (≠ L304)
- binding aspartic acid: S268 (= S275), S269 (= S276), T306 (= T313), G346 (= G353), I347 (= I354), A350 (= A357), G351 (≠ S358), D380 (= D387), R383 (= R390), T384 (= T391)
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
38% identity, 88% coverage: 42:411/422 of query aligns to 47:418/425 of O59010
- S65 (≠ A60) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ S274) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ SSS 274:276) binding L-aspartate
- M311 (= M310) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T313) binding L-aspartate
- V355 (≠ I354) binding L-aspartate
- D394 (= D387) binding L-aspartate
- M395 (= M388) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R390) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N394) binding L-aspartate
- D405 (= D398) mutation to N: Strongly decreased affinity for aspartate.
5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
37% identity, 91% coverage: 30:413/422 of query aligns to 37:395/397 of 5mjuA
- binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (≠ M65), S80 (≠ G73), G81 (= G75), G84 (= G78), Y91 (= Y85), M156 (≠ L162), F160 (≠ Y166), F286 (= F300), V290 (≠ L304)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (≠ V57), I148 (≠ M154), S262 (= S276), S263 (≠ A277), A292 (= A306), T293 (= T307), M296 (= M310), T299 (= T313), G329 (= G350), A336 (= A357), G337 (≠ S358), D366 (= D387), R369 (= R390), N373 (= N394)
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
37% identity, 95% coverage: 8:410/422 of query aligns to 15:417/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (= F40), F46 (= F40), P75 (≠ Q71), L91 (≠ M87), F95 (≠ I91), L130 (≠ I133), I133 (≠ V136), I159 (≠ L162), Y167 (= Y166), K196 (≠ Q192), G200 (≠ W196), I207 (≠ L203), F210 (= F206), L250 (≠ I246), I262 (≠ Y260), M269 (≠ I267), T334 (≠ D333), V335 (= V334), G336 (≠ T335), T340 (≠ M339), L343 (≠ V342), M399 (≠ T392)
- binding aspartic acid: S277 (= S275), S278 (= S276), T314 (= T313), G354 (= G353), A358 (= A357), G359 (≠ S358), D394 (= D387), R397 (= R390), T398 (= T391)
- binding sodium ion: Y89 (= Y85), T92 (= T88), S93 (= S89), G306 (= G305), T308 (= T307), N310 (= N309), N310 (= N309), M311 (= M310), D312 (= D311), S349 (= S348), I350 (= I349), T352 (≠ V351), N401 (= N394), V402 (≠ I395), D405 (= D398)
Sites not aligning to the query:
2nwwA Crystal structure of gltph in complex with tboa (see paper)
37% identity, 95% coverage: 8:409/422 of query aligns to 6:407/407 of 2nwwA
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
37% identity, 95% coverage: 8:409/422 of query aligns to 12:413/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G64), V83 (≠ L82), I157 (≠ L163), Y164 (= Y166), K193 (≠ Q192), T305 (= T307), I306 (≠ V308), I347 (= I349)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I9), M199 (= M198), S275 (= S276), T311 (= T313), G356 (≠ S358), L384 (= L380), D391 (= D387), R394 (= R390)
Sites not aligning to the query:
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
38% identity, 87% coverage: 42:410/422 of query aligns to 39:409/409 of 6bavA
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
38% identity, 87% coverage: 42:409/422 of query aligns to 39:408/408 of 6bauA
- binding cysteine: S270 (= S276), M303 (= M310), T306 (= T313), A345 (= A352), G346 (= G353), V347 (≠ I354), G351 (≠ S358), D386 (= D387), C389 (≠ R390), T390 (= T391), N393 (= N394)
8cv2A Human excitatory amino acid transporter 3 (eaat3) in an outward facing sodium-bound state (see paper)
36% identity, 96% coverage: 3:407/422 of query aligns to 2:421/433 of 8cv2A
- binding sodium ion: Y85 (= Y85), T88 (= T88), T89 (≠ S89), G319 (= G305), A320 (= A306), N323 (= N309), N323 (= N309), M324 (= M310), D325 (= D311), N408 (= N394), D412 (= D398)
Q10901 Excitatory amino acid transporter; Sodium-dependent glutamate/ aspartate transporter from Caenorhabditis elegans (see paper)
34% identity, 89% coverage: 40:413/422 of query aligns to 54:465/503 of Q10901
- N177 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- N187 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
6x2zA Heaat3-ofs-asp (see paper)
36% identity, 97% coverage: 6:416/422 of query aligns to 3:418/419 of 6x2zA