SitesBLAST
Comparing WP_207545101.1 NCBI__GCF_900115975.1:WP_207545101.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
47% identity, 98% coverage: 3:427/434 of query aligns to 2:419/426 of P22256
- I50 (= I51) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
- GS 111:112 (≠ GA 112:113) binding pyridoxal 5'-phosphate
- E211 (= E219) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
- V241 (= V249) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
- Q242 (= Q250) binding pyridoxal 5'-phosphate
- K268 (= K276) modified: N6-(pyridoxal phosphate)lysine
- T297 (= T305) binding pyridoxal 5'-phosphate
1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
47% identity, 98% coverage: 3:427/434 of query aligns to 1:418/425 of 1sffA
- active site: V18 (≠ I20), Y137 (≠ F139), E205 (= E214), D238 (= D247), Q241 (= Q250), K267 (= K276), T296 (= T305), R397 (= R406)
- binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (≠ M80), G110 (= G112), S111 (≠ A113), Y137 (≠ F139), H138 (= H140), R140 (= R142), E205 (= E214), D238 (= D247), V240 (= V249), Q241 (= Q250), K267 (= K276), T296 (= T305)
- binding sulfate ion: N152 (≠ K154), Y393 (≠ S402)
1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
47% identity, 98% coverage: 3:427/434 of query aligns to 1:418/425 of 1sf2A
- active site: V18 (≠ I20), Y137 (≠ F139), E205 (= E214), D238 (= D247), Q241 (= Q250), K267 (= K276), T296 (= T305), R397 (= R406)
- binding pyridoxal-5'-phosphate: G110 (= G112), S111 (≠ A113), Y137 (≠ F139), H138 (= H140), E205 (= E214), D238 (= D247), V240 (= V249), Q241 (= Q250), K267 (= K276)
- binding sulfate ion: N152 (≠ K154), Y393 (≠ S402)
1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
47% identity, 98% coverage: 3:427/434 of query aligns to 1:418/425 of 1szkA
- active site: V18 (≠ I20), Y137 (≠ F139), E205 (= E214), D238 (= D247), Q241 (= Q250), K267 (= K276), T296 (= T305), R397 (= R406)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G112), S111 (≠ A113), Y137 (≠ F139), H138 (= H140), E205 (= E214), D238 (= D247), V240 (= V249), Q241 (= Q250), K267 (= K276)
P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see paper)
44% identity, 98% coverage: 4:430/434 of query aligns to 2:420/421 of P50457
- K267 (= K276) mutation to A: No GABA-AT activity.
3q8nC Crystal structure of 4-aminobutyrate transaminase from mycobacterium smegmatis (see paper)
47% identity, 98% coverage: 7:432/434 of query aligns to 19:438/439 of 3q8nC
- active site: V32 (≠ I20), Y151 (≠ F139), E221 (= E214), D254 (= D247), Q257 (= Q250), K283 (= K276), T312 (= T305), R412 (= R406)
- binding 4-oxobutanoic acid: G124 (= G112), A125 (= A113), V256 (= V249), K283 (= K276)
4atqF Gaba-transaminase a1r958 in complex with external aldimine plp-gaba adduct (see paper)
48% identity, 97% coverage: 8:429/434 of query aligns to 23:441/444 of 4atqF
- active site: V35 (≠ I20), Y154 (≠ F139), E226 (= E214), D259 (= D247), Q262 (= Q250), K288 (= K276), T317 (= T305), R418 (= R406)
- binding gamma-amino-butanoic acid: M95 (= M80), Y154 (≠ F139), R157 (= R142), E231 (= E219), K288 (= K276), G316 (= G304)
- binding pyridoxal-5'-phosphate: G127 (= G112), A128 (= A113), Y154 (≠ F139), H155 (= H140), D259 (= D247), V261 (= V249)
6j2vA Gaba aminotransferase from corynebacterium glutamicum (see paper)
43% identity, 97% coverage: 11:429/434 of query aligns to 26:435/440 of 6j2vA
- active site: L35 (≠ I20), Y154 (≠ F139), D256 (= D247), K285 (= K276)
- binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]butanoic acid: G127 (= G112), A128 (= A113), Y154 (≠ F139), H155 (= H140), R157 (= R142), E223 (= E214), E228 (= E219), D256 (= D247), I258 (≠ V249), K285 (= K276), G313 (= G304), T314 (= T305)
5wyaA Structure of amino acid racemase, 2.65 a (see paper)
39% identity, 98% coverage: 6:432/434 of query aligns to 6:432/439 of 5wyaA
- active site: A20 (≠ I20), Y140 (≠ F139), E215 (= E214), D248 (= D247), N251 (≠ Q250), K278 (= K276), T307 (= T305), R406 (= R406)
- binding (2S,3S)-3-methyl-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]pentanoic acid: A52 (≠ I51), Y82 (≠ V81), S112 (= S111), G113 (= G112), S114 (≠ A113), Y140 (≠ F139), H141 (= H140), E215 (= E214), D248 (= D247), V250 (= V249), N251 (≠ Q250), K278 (= K276), F306 (≠ G304), T307 (= T305), R406 (= R406)
4ysnC Structure of aminoacid racemase in complex with plp (see paper)
39% identity, 98% coverage: 6:432/434 of query aligns to 15:441/448 of 4ysnC
- active site: A29 (≠ I20), Y149 (≠ F139), E224 (= E214), D257 (= D247), N260 (≠ Q250), K287 (= K276), T316 (= T305), R415 (= R406)
- binding pyridoxal-5'-phosphate: S121 (= S111), G122 (= G112), S123 (≠ A113), Y149 (≠ F139), H150 (= H140), E224 (= E214), D257 (= D247), V259 (= V249), K287 (= K276), F315 (≠ G304), T316 (= T305)
5wyfA Structure of amino acid racemase, 2.12 a (see paper)
39% identity, 98% coverage: 6:432/434 of query aligns to 8:434/446 of 5wyfA