SitesBLAST
Comparing WP_208610120.1 NCBI__GCF_900142125.1:WP_208610120.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 10 hits to proteins with known functional sites (download)
8w78A Structure of drosophila melanogaster l-2-hydroxyglutarate dehydrogenase in complex with fad and 2-oxoglutarate (see paper)
39% identity, 96% coverage: 18:402/402 of query aligns to 15:409/410 of 8w78A
- binding 2-oxoglutaric acid: S48 (= S51), H52 (= H55), Y245 (= Y245), F254 (= F254), L255 (= L255), H258 (= H258), R349 (= R349)
- binding flavin-adenine dinucleotide: E34 (≠ D37), K35 (= K38), H42 (= H45), Q43 (= Q46), S44 (≠ T47), N47 (= N50), S48 (= S51), V50 (= V53), H52 (= H55), V173 (≠ I175), G203 (≠ A203), G204 (= G204), Q206 (≠ M206), R349 (= R349), P387 (= P380), G388 (≠ A381), A389 (= A382), T390 (= T383)
Sites not aligning to the query:
8w7fB Structure of drosophila melanogaster l-2-hydroxyglutarate dehydrogenase bound with fad and a sulfate ion (see paper)
39% identity, 96% coverage: 18:402/402 of query aligns to 15:411/412 of 8w7fB
- binding flavin-adenine dinucleotide: E34 (≠ D37), K35 (= K38), H42 (= H45), Q43 (= Q46), S44 (≠ T47), H46 (= H49), N47 (= N50), S48 (= S51), V50 (= V53), H52 (= H55), V173 (≠ I175), G205 (≠ A203), G206 (= G204), Q208 (≠ M206), Y231 (= Y229), V350 (≠ I348), R351 (= R349), P389 (= P380), G390 (≠ A381), A391 (= A382), T392 (= T383)
Sites not aligning to the query:
3dmeA Crystal structure of conserved exported protein from bordetella pertussis. Northeast structural genomics target ber141
30% identity, 66% coverage: 7:272/402 of query aligns to 4:271/366 of 3dmeA
- binding flavin-adenine dinucleotide: I8 (≠ V11), G9 (= G12), G11 (= G14), V12 (≠ I15), V13 (= V16), E32 (≠ D37), A33 (≠ K38), T41 (≠ Q46), S42 (≠ T47), R44 (≠ H49), N45 (= N50), S46 (= S51), V48 (= V53), H50 (= H55), P170 (≠ E174), L171 (≠ I175), A203 (= A203)
Sites not aligning to the query:
4x9mA Oxidized l-alpha-glycerophosphate oxidase from mycoplasma pneumoniae with fad bound (see paper)
26% identity, 73% coverage: 7:300/402 of query aligns to 5:300/384 of 4x9mA
- binding flavin-adenine dinucleotide: G10 (= G12), G12 (= G14), I14 (≠ V16), E33 (≠ D37), K34 (= K38), E41 (≠ H45), T42 (≠ Q46), S43 (≠ T47), A45 (≠ H49), N46 (= N50), S47 (= S51), V49 (= V53), H51 (= H55), E176 (= E174), V177 (≠ I175), A209 (= A203), G210 (= G204), Y212 (≠ M206), Y234 (= Y229)
- binding sn-glycerol-3-phosphate: S47 (= S51), H51 (= H55), K258 (≠ L255), G259 (= G256)
Sites not aligning to the query:
P75063 Glycerol 3-phosphate oxidase; GlpO; L-alpha-glycerophosphate oxidase; EC 1.1.3.21 from Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) (see paper)
26% identity, 73% coverage: 7:300/402 of query aligns to 5:300/384 of P75063
- I14 (≠ V16) binding FAD
- E33 (≠ D37) binding FAD
- TS 42:43 (≠ QT 46:47) binding FAD
- SGV 47:49 (= SGV 51:53) binding FAD
- V177 (≠ I175) binding FAD
Sites not aligning to the query:
- 346:347 binding FAD
- 352 binding FAD
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
22% identity, 56% coverage: 5:228/402 of query aligns to 1:223/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (≠ V11), G8 (= G12), G10 (= G14), V11 (≠ I15), I12 (≠ V16), V30 (≠ L34), E31 (≠ K38), K32 (≠ E39), E38 (≠ H45), A39 (≠ Q46), S40 (≠ T47), A43 (≠ N50), G45 (= G52), L46 (≠ V53), V171 (≠ I175), G200 (≠ A203), G201 (= G204), W203 (≠ M206)
Sites not aligning to the query:
3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
25% identity, 56% coverage: 8:231/402 of query aligns to 4:226/827 of 3gsiA
- active site: H222 (≠ G227)
- binding flavin-adenine dinucleotide: G10 (= G14), I11 (= I15), V12 (= V16), D32 (≠ E39), Q33 (= Q40), G41 (= G48), S42 (≠ H49), T43 (≠ N50), H45 (= H55), P47 (vs. gap), L49 (≠ V58), T170 (≠ E174), V171 (≠ I175), A200 (= A203), G201 (= G204), W203 (≠ M206), H222 (≠ G227)
Sites not aligning to the query:
- active site: 256, 549
- binding flavin-adenine dinucleotide: 256, 330, 331, 332, 357, 358, 359, 360
- binding magnesium ion: 254, 409
- binding (6s)-5,6,7,8-tetrahydrofolate: 505, 536, 551, 563, 629, 648, 655, 696
1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
25% identity, 56% coverage: 8:231/402 of query aligns to 5:227/828 of 1pj6A
- active site: H223 (≠ G227)
- binding flavin-adenine dinucleotide: G9 (= G12), G11 (= G14), I12 (= I15), V13 (= V16), D33 (≠ E39), Q34 (= Q40), G42 (= G48), S43 (≠ H49), T44 (≠ N50), H46 (= H55), P48 (vs. gap), L50 (≠ V58), V172 (≠ I175), A201 (= A203), G202 (= G204), W204 (≠ M206), H223 (≠ G227)
Sites not aligning to the query:
1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
25% identity, 56% coverage: 8:231/402 of query aligns to 4:226/827 of 1pj7A
- active site: H222 (≠ G227)
- binding flavin-adenine dinucleotide: G8 (= G12), G10 (= G14), I11 (= I15), V12 (= V16), D32 (≠ E39), Q33 (= Q40), G41 (= G48), S42 (≠ H49), T43 (≠ N50), H45 (= H55), P47 (vs. gap), L49 (≠ V58), T170 (≠ E174), V171 (≠ I175), A200 (= A203), G201 (= G204), W203 (≠ M206), H222 (≠ G227)
Sites not aligning to the query:
- active site: 256, 549
- binding flavin-adenine dinucleotide: 256, 331, 357, 358, 359, 360
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: 505, 536, 549, 551, 563, 629, 648, 655, 696
Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
25% identity, 56% coverage: 8:231/402 of query aligns to 7:229/830 of Q9AGP8
- IV 14:15 (= IV 15:16) binding FAD
- DQ 35:36 (≠ EQ 39:40) binding FAD
- STS---H 45:48 (≠ HNSGVVH 49:55) binding FAD
- L52 (≠ V58) binding FAD
- V174 (≠ I175) binding FAD
- H225 (≠ G227) Important for catalytic activity; mutation to Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.
Sites not aligning to the query:
- 259 Important for catalytic activity; binding FAD; Y→F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.
- 360:363 binding FAD
- 539 binding (6S)-5,6,7,8-tetrahydrofolate
- 552 Important for catalytic activity; D→A: No effect on the activity.; D→N: Reduces activity 3-fold.
Query Sequence
>WP_208610120.1 NCBI__GCF_900142125.1:WP_208610120.1
MNQLKSDLVVVGNGIVGAATALRIKQDFPHLSVLMIDKEQSCATHQTGHNSGVVHAGVYY
QPGSLKADFCKRGAMLTKSFCLQNNIPFVQCGKLLVATNELELTRMEALAQRCHENGITT
VALSEQELKAKESKVSGLGALHVPATAITDFPKITETMVAHFCALGGQNLFGAEITQIKE
DGQSVRINTGQHLVETSALVVCAGLMADRLVRMMGVACDFQIIPFRGEYYRLAADLNDVV
KHLIYPIPDPDLPFLGVHLTPMVNGTVTVGPNAVLGWKREGYSTVNFNIKDVAEMLSFPG
FWKVLQNNFATGLKEMSDSLLLSGYLKRVQKYCPSIKATDLKPYPAGIRAQAVLSDGSLV
HDFLFKETERSLHVCNAPSPAATSAMPIGEYLAAKAAEKFRL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory