SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_210399235.1 NCBI__GCF_001579945.1:WP_210399235.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
66% identity, 99% coverage: 1:310/312 of query aligns to 7:315/318 of Q63XL8

query
sites
Q63XL8

M

L

A

M

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V

F

F

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RQ 99:100 (= RQ 93:94) binding ATP

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
66% identity, 96% coverage: 1:300/312 of query aligns to 3:297/299 of 6nfeB

query
sites
6nfeB

M

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binding adenosine-5'-diphosphate: F34 (= F32), D36 (= D34), E38 (= E36), R95 (= R93), Q96 (= Q94), H130 (= H128)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H128), D214 (= D217), D215 (= D218), I216 (≠ L219), D218 (= D221), T219 (= T222), A220 (= A223), T222 (= T225)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
66% identity, 96% coverage: 1:300/312 of query aligns to 3:296/298 of 6nfeA

query
sites
6nfeA

M

M

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M

V

I

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F

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D

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Q

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G

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binding adenosine-5'-diphosphate: F34 (= F32), D36 (= D34), E38 (= E36), R95 (= R93), Q96 (= Q94), H130 (= H128)
binding adenosine monophosphate: R98 (= R96), V100 (≠ P98), Y146 (= Y144), R175 (= R173), A178 (= A176), K181 (= K179)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H128), D213 (= D217), D214 (= D218), I215 (≠ L219), D217 (= D221), T218 (= T222), A219 (= A223), T221 (= T225)

6asvC E. Coli prpp synthetase (see paper)
62% identity, 99% coverage: 1:310/312 of query aligns to 2:311/311 of 6asvC

query
sites
6asvC

M

M

A

K

V

L

F

F

A

A

G

G

S

N

A

A

N

T

R

P

Q

E

L

L

A

A

H

Q

D

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I

I

A

A

R

N

H

R

M

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M

Y

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S

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L

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G

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D

A

A

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A

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V

G

G

R

R

F

F

S

S

D

D

G

G

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E

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L

I

L

N

E

E

N

N

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V

R

R

G

G

R

G

D

D

I

I

F

F

I

I

V

I

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Q

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T

S

C

P

A

P

P

A

T

N

N

E

D

H

N

V

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M

M

E

E

L

L

L

V

V

V

M

M

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V

D

D

A

A

C

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R

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A

A

A

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A

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Y

F

F

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G

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Y

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D

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I

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G

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D

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L

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F

binding phosphate ion: D168 (= D167), D218 (= D217), D219 (= D218), D222 (= D221), T223 (= T222), G224 (≠ A223), T226 (= T225), L227 (= L226)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
62% identity, 99% coverage: 1:310/312 of query aligns to 4:313/315 of P0A717

query
sites
P0A717

M

M

A

K

V

L

F

F

A

A

G

G

S

N

A

A

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H

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Y

V

T

P

S

L

L

G

G

R

D

A

A

S

A

V

V

G

G

R

R

F

F

S

S

D

D

G

G

E

E

T

V

T

S

V

V

E

Q

L

I

L

N

E

E

N

N

V

V

R

R

G

G

R

G

D

D

I

I

F

F

I

I

V

I

Q

Q

P

S

T

T

S

C

P

A

P

P

A

T

N

N

E

D

H

N

V

L

M

M

E

E

L

L

L

V

V

V

M

M

V

V

D

D

A

A

C

L

R

R

A

A

S

S

A

A

A

G

R

R

I

I

T

T

A

A

V

V

V

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

P

V

R

R

A

S

T

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

M

F

I

L

A

S

S

S

A

V

G

G

V

V

D

D

R

R

V

V

L

L

T

T

V

V

D
|
D

L

L

H

H

A

A

D

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

V

V

Y

F

A

G

S

S

P

P

V

I

L

L

G

E

D

D

V

M

W

L

R

Q

R

L

K

N

Y

L

D

D

N

N

L

P

M

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

R

R

A

A

R

R

A

A

L

I

A

A

K

K

R

L

L

L

D

N

D

D

A

T

E

D

L

M

A

A

I

I

D

D

K

K

R

R

P

P

R

R

A

A

N

N

E

V

S

S

E

Q

V

V

M

M

N

H

I

I

G

G

E

D

V

V

A

A

G

G

K

R

T

D

C

C

V

V

L

L

V

V

D
|
D

L

M

V

I

D
|
D

T

T

A

G

G

G

T

T

L

L

C

C

H

K

A

A

Q

E

A

A

L

L

K

K

K

E

A

R

G

G

A

A

Q

K

K

R

V

V

Y

F

A

A

Y

Y

I

A

T

T

H

H

A

P

V

I

L

F

S

S

G

G

P

N

A

A

I

A

E

N

R

N

I

L

T

R

K

N

S

S

D

V

L

I

D

D

E

E

L

V

V

V

T

C

D

D

T

T

I

I

P

P

L

L

S

S

E

D

A

E

A

I

R

K

Q

S

C

L

T

P

K

N

I

V

R

R

Q

T

L

L

S

T

V

L

A

S

G

G

L

M

L

L

A

A

E

E

T

A

M

I

R

R

I

I

R

S

D

N

E

E

S

S

V

I

S

S

S

A

L

M

Y

F

D129 (= D126) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D217) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D218) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (= D221) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
65% identity, 98% coverage: 1:306/312 of query aligns to 2:299/300 of 3dahC

query
sites
3dahC

M

L

A

M

V

V

F

F

A

T

G

G

S

N

A

A

N

N

R

P

Q

A

L

L

A

A

H

Q

D

E

I

V

A

V

R

K

H

I

M

L

M

G

V

I

P

P

L

L

G

G

R

K

A

A

S

M

V

V

G

S

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

T

I

T

Q

V

V

E

E

L

I

L

Q

E

E

N

N

V

V

R

R

G

G

R

K

D

D

I

V

F

F

I

V

V

L

Q

Q

P

S

T

T

S

C

P

A

P

P

A

T

N

N

E

D

H

N

V

L

M

M

E

E

L

L

L

M

V

I

M

M

V

V

D

D

A

A

C

L

R

K

R

R

A

A

S

S

A

A

A

G

R

R

I

I

T

T

A

A

V

A

V

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

P

P

R

R

A

S

T

A

R

R

V

V

P

A

I

I

T

S

A

A

K

K

L

V

V

V

A

A

N

N

M

M

I

L

A

E

S

I

A

A

G

G

V

V

D

E

R

R

V

I

L

I

T

T

V

M

D

D

L

L

H

H

A

A

D

D

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

V

D

D

N

N

V

I

Y

Y

A

A

S

T

P

P

V

I

L

L

G

G

D

D

V

L

W

R

R

K

R

Q

K

N

Y

Y

D

P

N

D

L

L

M

L

V

V

S

S

P

P

D

D

V

V

G

G

V

V

R

R

A

A

R

R

A

A

L

L

A

A

K

K

R

Q

L

L

D

-

D

N

A

C

E

D

L

L

A

A

I

I

D

D

K

K

R

R

P

-

R

-

A

-

N

-

E

-

S

-

E

-

V

V

M

M

N

N

I

I

G

G

E

E

V

V

A

E

G

G

K

R

T

T

C

C

V

V

L

I

V

M

D

D

L

M

V

V

D

D

T

T

A

A

G

G

T

T

L

L

C

C

H

K

A

A

Q

Q

A

V

L

L

K

K

K

E

A

R

G

G

A

A

Q

K

K

Q

V

V

Y

F

A

A

Y

Y

I

A

T

T

H

H

A

P

V

V

L

L

S

S

G

G

P

G

A

A

I

A

E

D

R

R

I

I

T

A

K

A

S

S

D

A

L

L

D

D

E

E

L

L

V

V

T

T

D

D

T

T

I

I

P

P

L

L

S

S

E

A

A

E

A

S

R

L

Q

A

C

C

T

P

K

K

I

I

R

R

Q

A

L

L

S

S

V

S

A

A

G

G

L

L

A

A

E

E

T

T

M

F

R

S

R

R

I

I

R

R

D

R

E

G

E

D

S

S

V

V

binding adenosine monophosphate: F33 (= F32), D35 (= D34), E37 (= E36), R94 (= R93), Q95 (= Q94)

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
63% identity, 98% coverage: 1:306/312 of query aligns to 3:308/308 of 4s2uA

query
sites
4s2uA

M

M

A

K

V

L

F

F

A

A

G

G

S

N

A

A

N

T

R

P

Q

E

L

L

A

A

H

Q

D

R

I

I

A

A

R

N

H

R

M

L

M

Y

V

T

P

S

L

L

G

G

R

D

A

A

S

A

V

V

G

G

R

R

F

F

S

S

D

D

G

G

E

E

T

V

T

S

V

V

E

Q

L

I

L

N

E

E

N

N

V

V

R

R

G

G

R

G

D

D

I

I

F

F

I

I

V

I

Q

Q

P

S

T

T

S

C

P

A

P

P

A

T

N

N

E

D

H

N

V

L

M

M

E

E

L

L

L

V

V

V

M

M

V

V

D

D

A

A

C

L

R

R

A

A

S

S

A

A

A

G

R

R

I

I

T

T

A

A

V

V

V

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

P

V

R

R

A

S

T

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

M

F

I

L

A

S

S

S

A

V

G

G

V

V

D

D

R

R

V

V

L

L

T

T

V

V

D

D

L

L

H

H

A

A

D

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

V

V

Y

F

A

G

S

S

P

P

V

I

L

L

G

E

D

D

V

M

W

L

R

Q

R

L

K

N

Y

L

D

D

N

N

L

P

M

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

R

R

A

A

R

R

A

A

L

I

A

A

K

K

R

L

L

L

D

N

D

D

A

T

E

D

L

M

A

A

I

I

D

D

K

K

R

R

P

P

R

R

A

A

N

N

E

V

S

S

E

Q

V

V

M

M

N

H

I

I

G

G

E

D

V

V

A

A

G

G

K

R

T

D

C

C

V

V

L

L

V

V

D

D

L

M

V

I

D
|
D

T

T

A

G

G

G

T
|
T

L

L

C

C

H

K

A

A

Q

E

A

A

L

L

K

K

K

E

A

R

G

G

A

A

Q

K

K

R

V

V

Y

F

A

A

Y

Y

I

A

T

T

H

H

A

P

V

I

L

F

S

S

G

G

P

N

A

A

I

A

E

N

R

N

I

L

T

R

K

N

S

S

D

V

L

I

D

D

E

E

L

V

V

V

T

C

D

D

T

T

I

I

P

P

L

L

S

S

E

D

A

E

A

I

R

K

Q

S

C

L

T

P

K

N

I

V

R

R

Q

T

L

L

S

T

V

L

A

S

G

G

L

M

L

L

A

A

E

E

T

A

M

I

R

R

I

I

R

S

D

N

E

E

S

S

V

I

binding magnesium ion: D223 (= D221), T227 (= T225)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
61% identity, 99% coverage: 1:310/312 of query aligns to 2:305/307 of 7xmvA

query
sites
7xmvA

M

M

A

K

V

L

F

F

A

A

G

G

S

N

A

A

N

T

R

P

Q

E

L

L

A

A

H

Q

D

R

I

I

A

A

R

N

H

R

M

L

M

Y

V

T

P

S

L

L

G

G

R

D

A

A

S

A

V

V

G

G

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

T

V

T

S

V

V

E

Q

L

I

L

N

E

E

N

N

V

V

R

R

G

G

R

G

D

D

I

I

F

F

I

I

V

I

Q

Q

P

S

T

T

S

C

P

A

P

P

A

T

N

N

E

D

H

N

V

L

M

M

E

E

L

L

L

V

V

V

M

M

V

V

D

D

A

A

C

L

R

R

A

A

S

S

A

A

A

G

R

R

I

I

T

T

A

A

V

V

V

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R
|
R

R

R

P
x
V

R
|
R

A

S

T

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

M

F

I

L

A

S

S

S

A

V

G

G

V

V

D

D

R

R

V

V

L

L

T

T

V

V

D

D

L

L

H
|
H

A

A

D
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

V

V

Y
x
F

A

G

S
|
S

P

P

V

I

L

L

G

E

D

D

V

M

W

L

R

Q

R

L

K

N

Y

L

D

D

N

N

L

P

M

I

V

V

S

S

P

P

D

D

V

I

G

G

V

V

V
|
V

R

R

A

A

R

R

A
|
A

L

I

A

A

K

K

R

L

L

L

D

N

D

D

A

T

E

D

L

M

A

A

I

I

D

D

K

K

R

R

P

-

R

-

A

-

N

-

E

-

S

-

E

Q

V

V

M

M

N

H

I

I

G

G

E

D

V

V

A

A

G

G

K

R

T

D

C

C

V

V

L

L

V

V

D
|
D

L
x
M

V

I

D
|
D

T
|
T

A

G

G
|
G

T
|
T

L

L

C

C

H

K

A

A

Q

E

A

A

L

L

K

K

K

E

A

R

G

G

A

A

Q

K

K

R

V

V

Y

F

A

A

Y

Y

I

A

T

T

H

H

A

P

V

I

L

F

S

S

G

G

P

N

A

A

I

A

E

N

R

N

I

L

T

R

K

N

S

S

D

V

L

I

D

D

E

E

L

V

V

V

T

C

D

D

T

T

I

I

P

P

L

L

S

S

E

D

A

E

A

I

R

K

Q

S

C

L

T

P

K

N

I

V

R

R

Q

T

L

L

S

T

V

L

A

S

G

G

L

M

L

L

A

A

E

E

T

A

M

I

R

R

I

I

R

S

D

N

E

E

S

S

V

I

S

S

S

A

L

M

Y

F

binding adenosine-5'-diphosphate: F33 (= F32), D35 (= D34), E37 (= E36), R94 (= R93), R97 (= R96), H129 (= H128)
binding adenosine monophosphate: R97 (= R96), V99 (≠ P98), R100 (= R99), E131 (≠ D130), F145 (≠ Y144), S147 (= S146), V173 (= V172), A177 (= A176)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D217), D213 (= D218), M214 (≠ L219), D216 (= D221), T217 (= T222), G219 (= G224), T220 (= T225)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
61% identity, 99% coverage: 1:310/312 of query aligns to 2:305/307 of 7xmuA

query
sites
7xmuA

M

M

A

K

V

L

F

F

A

A

G

G

S

N

A

A

N

T

R

P

Q

E

L

L

A

A

H

Q

D

R

I

I

A

A

R

N

H

R

M

L

M

Y

V

T

P

S

L

L

G

G

R

D

A

A

S

A

V

V

G

G

R

R

F
|
F

S

S

D
|
D

G

G

E
|
E

T

V

T

S

V

V

E

Q

L

I

L

N

E

E

N

N

V

V

R

R

G

G

R

G

D

D

I

I

F

F

I

I

V

I

Q

Q

P

S

T

T

S

C

P

A

P

P

A

T

N

N

E

D

H

N

V

L

M

M

E

E

L

L

L

V

V

V

M

M

V

V

D

D

A

A

C

L

R

R

A

A

S

S

A

A

A

G

R

R

I

I

T

T

A

A

V

V

V

I

P

P

Y

Y

F

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

R

P

V

R
|
R

A

S

T

A

R

R

V

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

M

F

I

L

A

S

S

S

A

V

G

G

V

V

D

D

R

R

V

V

L

L

T

T

V

V

D

D

L

L

H
|
H

A

A

D
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

V

V

D

D

N

N

V

V

Y
x
F

A

G

S
|
S

P

P

V

I

L

L

G

E

D

D

V

M

W

L

R

Q

R

L

K

N

Y

L

D

D

N

N

L

P

M

I

V

V

S

S

P

P

D
|
D

V

I

G

G

V

V

V
|
V

R

R

A

A

R

R

A

A

L

I

A

A

K

K

R

L

L

L

D

N

D

D

A

T

E

D

L

M

A

A

I

I

D

D

K

K

R

R

P

-

R

-

A

-

N

-

E

-

S

-

E

Q

V

V

M

M

N

H

I

I

G

G

E

D

V

V

A

A

G

G

K

R

T

D

C

C

V

V

L

L

V

V

D
|
D

D

D

L
x
M

V

I

D
|
D

T
|
T

A

G

G

G

T

T

L

L

C

C

H

K

A

A

Q

E

A

A

L

L

K

K

K

E

A

R

G

G

A

A

Q

K

K

R

V

V

Y

F

A

A

Y

Y

I

A

T

T

H

H

A

P

V

I

L

F

S

S

G

G

P

N

A

A

I

A

E

N

R

N

I

L

T

R

K

N

S

S

D

V

L

I

D

D

E

E

L

V

V

V

T

C

D

D

T

T

I

I

P

P

L

L

S

S

E

D

A

E

A

I

R

K

Q

S

C

L

T

P

K

N

I

V

R

R

Q

T

L

L

S

T

V

L

A

S

G

G

L

M

L

L

A

A

E

E

T

A

M

I

R

R

I

I

R

S

D

N

E

E

S

S

V

I

S

S

S

A

L

M

Y

F

binding adenosine-5'-diphosphate: F33 (= F32), D35 (= D34), E37 (= E36), R94 (= R93), Q95 (= Q94), R97 (= R96), R97 (= R96), R100 (= R99), H129 (= H128), E131 (≠ D130), F145 (≠ Y144), S147 (= S146), V173 (= V172)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D167), D212 (= D217), M214 (≠ L219), D216 (= D221), T217 (= T222)

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
54% identity, 99% coverage: 1:310/312 of query aligns to 10:316/317 of P14193

query
sites
P14193

M

L

A

K

V

I

F

F

A

S

G

L

S

N

A

S

N

N

R

P

Q

E

L

L

A

A

H

K

D

E

I

I

A

A

R

D

H

I

M

V

M

G

V

V

P

Q

L

L

G

G

R

K

A

C

S

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

T

V

T

Q

V

I

E

N

L

I

L

E

E

E

N

S

V

I

R

R

G

G

R

C

D

D

I

C

F

Y

I

I

V

I

Q

Q

P

S

T

T

S

S

P

D

P

P

A

V

N

N

E

E

H

H

V

I

M

M

E

E

L

L

V

I

M

M

V

V

D

D

A

A

C

L

R

K

R

R

A

A

S

S

A

A

A

K

R

T

I

I

T

N

A

I

V

V

V

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

R

K

P

A

R

R

A

-

T

S

R

R

V

E

P

P

I

I

T

T

A

A

K

K

L

L

V

F

A

A

N

N

M

L

I

L

A

E

S

T

A

A

G

G

V

A

D

T

R

R

V

V

L

I

T

A

V

L

D

D

L

L

H

H

A

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

V

L

Y

M

A

G

S

V

P

P

V

I

L

L

L

G

G

E

D

Y

V

F

W

E

R

G

R

K

K

N

Y

L

D

E

N

D

L

I

M

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

L

L

A

A

K

D

R

R

L

L

D

K

D

-

A

A

E

P

L

I

A

A

I

I

D

D

K
|
K

R

R

R
|
R

P

P

R
|
R

A

P

N
|
N

E

V

S

A

E
|
E

V

V

M

M

N

N

I

I

I

V

G

G

E

N

V

I

A

E

G

G

K

K

T

T

C

A

V

I

L

L

V

I

D

D

L

I

V

I

D
|
D

T
|
T

A
|
A

G
|
G

T
|
T

L

I

C

T

H

L

A

A

Q

N

A

A

L

L

K

V

K

E

A

N

G

G

A

A

Q

K

K

E

V

V

Y

Y

A

A

Y

C

I

C

T

T

H

H

A

P

V

V

L

L

S

S

G

G

P

P

A

A

I

V

E

E

R

R

I

I

T

N

K

N

S

S

D

T

L

I

D

K

E

E

L

L

V

V

T

T

D

N

T

S

I

I

P

K

L

L

S

P

E

E

A

-

A

E

R

K

Q

K

C

I

T

E

K

R

I

F

R

K

Q

Q

L

L

S

S

V

V

A

G

G

P

L

L

A

A

E

E

T

A

M

I

R

I

R

R

I

V

R

H

D

E

E

Q

S

S

V

V

S

S

S

Y

L

L

Y

F

RQ 102:103 (= RQ 93:94) binding ATP
K198 (= K191) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R193) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (= R195) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (= N197) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (= E200) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (= DTAGT 221:225) binding D-ribose 5-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
51% identity, 99% coverage: 1:310/312 of query aligns to 4:299/299 of 1ibsB

query
sites
1ibsB

M

L

A

K

V

I

F

F

A

S

G

L

S

N

A

S

N

N

R

P

Q

E

L

L

A

A

H

K

D

E

I

I

A

A

R

D

H

I

M

V

M

G

V

V

P

Q

L

L

G

G

R

K

A

C

S

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

T

V

T

Q

V

I

E

N

L

I

L

E

E

E

N

S

V

I

R

R

G

G

R

C

D

D

I

C

F

Y

I

I

V

I

Q

Q

P

S

T

T

S

S

P

D

P

P

A

V

N

N

E

E

H

H

V

I

M

M

E

E

L

L

V

I

M

M

V

V

D

D

A

A

C

L

R

K

R

R

A

A

S

S

A

A

A

K

R

T

I

I

T

N

A

I

V

V

V

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

K

P

-

R

-

A

-

T

S

R

R

V

E

P

P

I

I

T

T

A

A

K

K

L

L

V

F

A

A

N

N

M

L

I

L

A

E

S

T

A

A

G

G

V

A

D

T

R

R

V

V

L

I

T

A

V

L

D

D

L

L

H

H

A

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

V

L

Y

M

A

G

S

V

P

P

V

I

L

L

L

G

G

E

D

Y

V

F

W

E

R

G

R

K

K

N

Y

L

D

E

N

D

L

I

M

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

L

L

A

A

K

D

R

R

L

L

D

-

D

K

A

A

E

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

R

-

A

-

N

-

E

-

S

-

E

-

V

-

M

M

N

N

I

I

I

V

G

G

E

N

V

I

A

E

G

G

K

K

T

T

C

A

V

I

L

L

V

I

D

D

L

I

V

I

D

D

T

T

A

A

G

G

T

T

L

I

C

T

H

L

A

A

Q

N

A

A

L

L

K

V

K

E

A

N

G

G

A

A

Q

K

K

E

V

V

Y

Y

A

A

Y

C

I

C

T

T

H

H

A

P

V

V

L

L

S

S

G

G

P

P

A

A

I

V

E

E

R

R

I

I

T

N

K

N

S

S

D

T

L

I

D

K

E

E

L

L

V

V

T

T

D

N

T

S

I

I

P

K

L

L

S

P

E

E

A

-

A

E

R

K

Q

K

C

I

T

E

K

R

I

F

R

K

Q

Q

L

L

S

S

V

V

A

G

G

P

L

L

A

A

E

E

T

A

M

I

R

I

R

R

I

V

R

H

D

E

E

Q

S

S

V

V

S

S

S

Y

L

L

Y

F

binding methyl phosphonic acid adenosine ester: R96 (= R93), Q97 (= Q94), R99 (= R96)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
51% identity, 99% coverage: 1:310/312 of query aligns to 2:297/297 of 1ibsA

query
sites
1ibsA

M

L

A

K

V

I

F

F

A

S

G

L

S

N

A

S

N

N

R

P

Q

E

L

L

A

A

H

K

D

E

I

I

A

A

R

D

H

I

M

V

M

G

V

V

P

Q

L

L

G

G

R

K

A

C

S

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

T

V

T

Q

V

I

E

N

L

I

L

E

E

E

N

S

V

I

R

R

G

G

R

C

D

D

I

C

F

Y

I

I

V

I

Q

Q

P

S

T

T

S

S

P

D

P

P

A

V

N

N

E

E

H

H

V

I

M

M

E

E

L

L

V

I

M

M

V

V

D

D

A

A

C

L

R

K

R

R

A

A

S

S

A

A

A

K

R

T

I

I

T

N

A

I

V

V

V

I

P

P

Y

Y

F

Y

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

R

K

P

-

R

-

A

-

T

S

R

R

V

E

P

P

I

I

T

T

A

A

K

K

L

L

V

F

A

A

N

N

M

L

I

L

A

E

S

T

A

A

G

G

V

A

D

T

R

R

V

V

L

I

T

A

V

L

D

D

L

L

H
|
H

A

A

D

P

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

V

I

D

D

N

H

V

L

Y

M

A

G

S

V

P

P

V

I

L

L

L

G

G

E

D

Y

V

F

W

E

R

G

R

K

K

N

Y

L

D

E

N

D

L

I

M

V

V

I

V

V

S

S

P

P

D

D

V

H

G

G

V

V

V

T

R

R

A

A

R

R

A

K

L

L

A

A

K

D

R

R

L

L

D

-

D

K

A

A

E

P

L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

R

-

A

-

N

-

E

-

S

-

E

-

V

-

M

M

N

N

I

I

I

V

G

G

E

N

V

I

A

E

G

G

K

K

T

T

C

A

V

I

L

L

V

I

D

D

L

I

V

I

D
|
D

T
|
T

A
|
A

G

G

T
|
T

L

I

C

T

H

L

A

A

Q

N

A

A

L

L

K

V

K

E

A

N

G

G

A

A

Q

K

K

E

V

V

Y

Y

A

A

Y

C

I

C

T

T

H

H

A

P

V

V

L

L

S

S

G

G

P

P

A

A

I

V

E

E

R

R

I

I

T

N

K

N

S

S

D

T

L

I

D

K

E

E

L

L

V

V

T

T

D

N

T

S

I

I

P

K

L

L

S

P

E

E

A

-

A

E

R

K

Q

K

C

I

T

E

K

R

I

F

R

K

Q

Q

L

L

S

S

V

V

A

G

G

P

L

L

A

A

E

E

T

A

M

I

R

I

R

R

I

V

R

H

D

E

E

Q

S

S

V

V

S

S

S

Y

L

L

Y

F

binding methyl phosphonic acid adenosine ester: R94 (= R93), Q95 (= Q94), R97 (= R96), H126 (= H128)
binding sulfate ion: D209 (= D221), T210 (= T222), A211 (= A223), T213 (= T225)

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
51% identity, 99% coverage: 1:310/312 of query aligns to 2:295/295 of 1dkuA

query
sites
1dkuA

M

L

A

K

V

I

F

F

A

S

G

L

S

N

A

S

N

N

R

P

Q

E

L

L

A

A

H

K

D

E

I

I

A

A

R

D

H

I

M

V

M

G

V

V

P

Q

L

L

G

G

R

K

A

C

S

S

V

V

G

T

R

R

F

F

S

S

D

D

G

G

E

E

T

V

T

Q

V

I

E

N

L

I

L

E

E

E

N

S

V

I

R

R

G

G

R

C

D

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C

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Y

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Y

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Y

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R

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x
K

P

A

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-

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x
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R

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E

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P

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A

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K

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A

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A

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L

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D

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-

D

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A

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L

I

A

A

I

I

D

D

K

K

R

R

R

-

P

-

R

-

A

-

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-

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-

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-

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-

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-

M

M

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N

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A

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A

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-

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-

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-

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Q

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E

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S

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x
F

binding methyl phosphonic acid adenosine ester: R94 (= R93), H128 (= H128)
binding phosphomethylphosphonic acid adenosyl ester: K98 (≠ R97), S101 (≠ T101), R102 (= R102), D141 (= D141), S290 (= S305), V291 (= V306), S292 (= S307), F295 (≠ Y310)

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
47% identity, 99% coverage: 1:310/312 of query aligns to 4:313/318 of P60891

query
sites
P60891

M

I

A

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I

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F

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G

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x
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A

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L

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A

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N

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A

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L

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x
S

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F

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I

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V

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N
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Y

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A

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L

L

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-

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-

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W

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x
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K

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A

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A

M

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S

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L

L

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F

S16 (≠ A13) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (= D49) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (= N112) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (= L127) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ D130) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (= V140) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (= N142) mutation to H: No effect on catalytic activity.
Y146 (= Y144) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ K179) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (= A187) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ D190) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ E200) to H: in a breast cancer sample; somatic mutation
V219 (= V216) to G: in a breast cancer sample; somatic mutation
H231 (= H228) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
47% identity, 99% coverage: 1:310/312 of query aligns to 3:312/316 of 8dbkB

query
sites
8dbkB

M

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A

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A

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