SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_211239097.1 NCBI__GCF_000526715.1:WP_211239097.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
67% identity, 99% coverage: 1:293/297 of query aligns to 1:292/303 of P16703

query
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P16703

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N71 (= N72) binding pyridoxal 5'-phosphate
S255 (= S256) binding pyridoxal 5'-phosphate

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
67% identity, 99% coverage: 1:293/297 of query aligns to 1:292/294 of 2bhtA

query
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2bhtA

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active site: K41 (= K42), S69 (= S70), Q199 (= Q200), G203 (= G204), S255 (= S256), C280 (= C281)
binding pyridoxal-5'-phosphate: K41 (= K42), N71 (= N72), M173 (= M174), G174 (= G175), T175 (= T176), T176 (= T177), T178 (= T179), G208 (= G209), S255 (= S256), C280 (= C281)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
43% identity, 97% coverage: 8:294/297 of query aligns to 12:307/310 of 5xoqA

query
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5xoqA

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binding : T72 (= T69), S73 (= S70), G74 (= G71), T76 (= T73), M123 (= M120), Q144 (= Q141), R218 (vs. gap), H219 (vs. gap), Q222 (≠ R211), G223 (≠ R212), A226 (≠ K215)

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
42% identity, 98% coverage: 4:293/297 of query aligns to 7:305/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K42), S266 (= S256), P293 (≠ C281)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K42), T71 (= T69), S72 (= S70), N74 (= N72), T75 (= T73), Q144 (= Q141), V177 (≠ M174), G178 (= G175), T179 (= T176), G180 (≠ T177), T182 (= T179), G222 (vs. gap), I223 (vs. gap), S266 (= S256), P293 (≠ C281), D294 (= D282)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
42% identity, 98% coverage: 4:293/297 of query aligns to 7:305/310 of P9WP55

query
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P9WP55

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K

A

T

V

N

A

S

D

V

I

I

V

L

-

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

N

A

M

M

I

L

K

Q

Q

A

A

A

E

E

L

Q

R

A

G

G

E

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

V

V

A

C

A

A

M

A

L

R

G

G

Y

Y

K

R

M

C

K

V

L

L

I

T

M

M

P

P

D

E

N

T

M

M

S

S

M

L

E

E

R

R

K

R

A

M

S

L

M

L

A

R

A

A

Y

Y

G

G

A

A

E

E

L

L

I

I

L

L

V

T

S

P

K

G

E

A

E

D

G

G

M

M

E

S

G

G

A

A

R

I

D

A

L

K

A

A

Q

E

K

E

M

L

-

A

Q

K

T

T

E

D

G

Q

Q

R

G

Y

M

F

V

V

L

P

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

L

A

S

I

H

H

Y

R

F

V

T

T

G

A

P

E

E

E

I

V

W

W

H

R

D

D

T

T

E

D

G

G

K

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M

V

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

M

T

G

G

T

V

S

A

M

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

P

P

D

S

V

A

Q

R

V

F

V

V

G

A

V

V

Q

E

P

P

T

A

E

A

G

S

S

P

S

V

I

L

P

S

G

G

I

G

R

Q

R

K

W

G

P

P

K

H

E

P

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

V

P

P

T

P

I

V

Y

L

D

D

D

Q

S

D

R

L

V

V

D

D

R

E

T

I

I

I

D

T

M

V

S

G

Q

N

S

E

L

D

A

A

E

L

D

N

T

V

M

A

I

R

R

R

M

L

A

A

K

R

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

M

T

A

V

A

A

L

L

Q

Q

I

V

A

A

N

R

E

R

V

P

D

E

N

N

A

A

-

G

-

K

L

L

I

I

V

V

T

V

I

V

V

L

C

P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

K44 (= K42) modified: N6-(pyridoxal phosphate)lysine
N74 (= N72) binding pyridoxal 5'-phosphate
GTGGT 178:182 (≠ GTTGT 175:179) binding pyridoxal 5'-phosphate
S266 (= S256) binding pyridoxal 5'-phosphate

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
42% identity, 96% coverage: 4:288/297 of query aligns to 7:300/300 of 3zeiA

query
sites
3zeiA

L

I

M

T

D

Q

V

L

V

I

G

G

N

R

T

T

P

P

L

L

I

V

K

R

V

L

Q

R

R

R

L

V

V

T

N

D

P

G

K

A

T

V

N

A

S

D

V

I

I

V

L

-

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

N

A

M

M

I

L

K

Q

Q

A

A

A

E

E

L

Q

R

A

G

G

E

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

V

V

A

C

A

A

M

A

L

R

G

G

Y

Y

K

R

M

C

K

V

L

L

I

T

M

M

P

P

D

E

N

T

M

M

S

S

M

L

E

E

R

R

K

R

A

M

S

L

M

L

A

R

A

A

Y

Y

G

G

A

A

E

E

L

L

I

I

L

L

V

T

S

P

K

G

E

A

E

D

G

G

M

M

E

S

G

G

A

A

R
x
I

D

A

L

K

A

A

Q

E

K

E

M

L

-

A

Q

K

T

T

E

D

G

Q

Q

R

G

Y

M

F

V

V

L

P

D

Q

Q
|
Q

F
|
F

A

E

N

N

P

P

D

A

N

N

P

P

L

A

S

I

H

H

Y

R

F

V

T

T

G

A

P

E

E

E

I

V

W

W

H

R

D

D

T

T

E

D

G

G

K

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

A

M

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

P

P

D

S

V

A

Q

R

V

F

V

V

G

A

V

V

Q

E

P

P

T

A

E

A

G

S

S

P

S

V

I

L

P

S

G

G

I

G

R

Q

R
x
K

W

G

P

P

K

H

E

P

-

I

-

Q

-
x
G

-

I

-

G

-
x
A

-

G

Y
x
F

L

V

P

P

T

P

I

V

Y

L

D

D

D

Q

S

D

R

L

V

V

D

D

R

E

T

I

I

I

D

T

M

V

S

G

Q

N

S

E

L

D

A

A

E

L

D

N

T

V

M

A

I

R

R

R

M

L

A

A

K

R

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

M

T

A

V

A

A

L

L

Q

Q

I

V

A

A

N

R

E

R

V

P

D

E

N

N

A

A

-

G

-

K

L

L

I

I

V

V

T

V

I

V

V

L

C
x
P

D
|
D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K42), S266 (= S256), P293 (≠ C281)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T69), S72 (= S70), I126 (≠ R124), Q144 (= Q141), F145 (= F142), K215 (≠ R212), G222 (vs. gap), A225 (vs. gap), F227 (≠ Y217)
binding pyridoxal-5'-phosphate: K44 (= K42), N74 (= N72), V177 (≠ M174), G178 (= G175), T179 (= T176), G180 (≠ T177), T182 (= T179), G222 (vs. gap), S266 (= S256), P293 (≠ C281), D294 (= D282)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
42% identity, 96% coverage: 4:288/297 of query aligns to 7:300/300 of 2q3cA

query
sites
2q3cA

L

I

M

T

D

Q

V

L

V

I

G

G

N

R

T

T

P

P

L

L

I

V

K

R

V

L

Q

R

R

R

L

V

V

T

N

D

P

G

K

A

T

V

N

A

S

D

V

I

I

V

L

-

A

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

N

A

M

M

I

L

K

Q

Q

A

A

A

E

E

L

Q

R

A

G

G

E

L

I

I

K

K

P

P

G

D

D

T

T

I

L

I

I

L

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

V

V

A

C

A

A

M

A

L

R

G

G

Y

Y

K

R

M

C

K

V

L

L

I

T

M

M

P

P

D

E

N

T

M

M

S

S

M

L

E

E

R

R

K

R

A

M

S

L

M

L

A

R

A

A

Y

Y

G

G

A

A

E

E

L

L

I

I

L

L

V

T

S

P

K

G

E

A

E

D

G

G

M
|
M

E

S

G

G

A

A

R

I

D

A

L

K

A

A

Q

E

K

E

M

L

-

A

Q

K

T

T

E

D

G

Q

Q

R

G

Y

M

F

V

V

L

P

D

Q

Q
|
Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

L

A

S

I

H

H

Y

R

F

V

T

T

G

A

P

E

E

E

I

V

W

W

H

R

D

D

T

T

E

D

G

G

K

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M

V

G

G

T

T

T

G

G

G

T

T

I

I

M

T

G

G

T

V

S

A

M

Q

Y

V

L

I

K

K

E

E

Q

R

N

K

P

P

D

S

V

A

Q

R

V

F

V

V

G

A

V

V

Q

E

P

P

T

A

E

A

G

S

S

P

S

V

I

L

P

S

G

G

I

G

R

Q

R
x
K

W

G

P

P

K

H

E

P

-

I

-

Q

-
x
G

-

I

-

G

-
x
A

-

G

Y

F

L

V

P

P

T

P

I

V

Y

L

D

D

D

Q

S

D

R

L

V

V

D

D

R

E

T

I

I

I

D

T

M

V

S

G

Q

N

S

E

L

D

A

A

E

L

D

N

T

V

M

A

I

R

R

R

M

L

A

A

K

R

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

M

T

A

V

A

A

L

L

Q

Q

I

V

A

A

N

R

E

R

V

P

D

E

N

N

A

A

-

G

-

K

L

L

I

I

V

V

T

V

I

V

V

L

C
x
P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K42), S266 (= S256), P293 (≠ C281)
binding : T71 (= T69), S72 (= S70), G73 (= G71), T75 (= T73), M122 (= M120), Q144 (= Q141), K215 (≠ R212), G222 (vs. gap), A225 (vs. gap)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
43% identity, 96% coverage: 8:293/297 of query aligns to 7:301/302 of 2efyA

query
sites
2efyA

V

I

G

G

N

K

T

T

P

P

L

V

I

V

K

R

V

L

Q

A

R

K

L

V

V

V

N

E

P

P

K

D

T

M

N

A

S

E

V

V

I

W

L

V

A

-

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

N

Y

M

M

I

I

K

K

Q

D

A

A

E

E

L

E

R

R

G

G

E

I

I

L

K

R

P

P

G

G

D

S

-

G

-

Q

T

V

L

I

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

V

I

A

A

M

S

L

R

G

G

Y

Y

K

R

M

L

K

I

L

L

I

T

M

M

P

P

D

A

N

Q

M

M

S

S

M

E

E

E

R

R

K

K

A

R

S

V

M

L

A

K

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

V

T

S

D

K

P

E

E

E

R

G

R

M

M

E

L

G

A

A

A

R

R

D

E

L

E

A

A

Q

L

K

R

M

L

Q

K

T

E

E

E

G

L

Q

G

G

A

M

F

V

M

L

P

D

D

Q
|
Q

F

F

A

K

N

N

P

P

D

A

N

N

P

V

L

R

S

A

H

H

Y

Y

F

E

T

T

G

G

P

P

E

E

I

L

W

Y

H

E

D

A

T

L

E

E

G

G

K

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

A

G

M

S

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

G

M

R

Y

Y

L

L

K

K

E

E

Q

R

N

I

P

P

D

H

V

V

Q

K

V

V

V

I

G

A

V

V

Q
x
E

P

P

T

A

E

R

G
x
S

S

N

S

V

I

L

P

S

-

G

-

K

-

M

-

G

-

Q

-

H

G

G

I

F

R

Q

R
x
G

W
x
M

P

G

K
x
P

E

G

Y

F

L

I

P

P

T

E

I

N

Y

L

D

D

D

L

S

S

R

L

V

L

D

D

R

G

T

V

I

I

D

Q

M

V

S

W

Q

E

S

E

L

D

A

A

E

F

D

P

T

L

M

A

I

R

R

R

M

L

A

A

K

R

E

E

G

G

I

L

F

F

A

L

G

G

V

M

S
|
S

S

S

G

G

A

I

M

V

A

W

A

A

L

L

Q

Q

I

V

A

A

N

R

E

E

V

L

D

G

-

P

N

G

A

K

L

R

I

V

V

A

T

C

I

I

V

S

C
x
P

D
|
D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K42), S70 (= S70), E200 (≠ Q200), S204 (≠ G204), S263 (= S256)
binding 5-oxohexanoic acid: T69 (= T69), G71 (= G71), T73 (= T73), Q141 (= Q141), G175 (= G175), G219 (≠ R212), M220 (≠ W213), P222 (≠ K215)
binding pyridoxal-5'-phosphate: K40 (= K42), N72 (= N72), Y172 (≠ S172), G175 (= G175), T176 (= T176), G177 (≠ T177), T179 (= T179), G219 (≠ R212), S263 (= S256), P289 (≠ C281), D290 (= D282)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
43% identity, 96% coverage: 8:293/297 of query aligns to 7:301/302 of 2ecqA

query
sites
2ecqA

V

I

G

G

N

K

T

T

P

P

L

V

I

V

K

R

V

L

Q

A

R

K

L

V

V

V

N

E

P

P

K

D

T

M

N

A

S

E

V

V

I

W

L

V

A

-

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

N

Y

M

M

I

I

K

K

Q

D

A

A

E

E

L

E

R

R

G

G

E

I

I

L

K

R

P

P

G

G

D

S

-

G

-

Q

T

V

L

I

I

V

E

E

A

P

T

T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

V

I

A

A

M

S

L

R

G

G

Y

Y

K

R

M

L

K

I

L

L

I

T

M

M

P

P

D

A

N

Q

M

M

S

S

M

E

E

E

R

R

K

K

A

R

S

V

M

L

A

K

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

V

T

S

D

K

P

E

E

E

R

G

R

M

M

E

L

G

A

A

A

R

R

D

E

L

E

A

A

Q

L

K

R

M

L

Q

K

T

E

E

E

G

L

Q

G

G

A

M

F

V

M

L

P

D

D

Q
|
Q

F

F

A

K

N

N

P

P

D

A

N

N

P

V

L

R

S

A

H

H

Y

Y

F

E

T

T

G

G

P

P

E

E

I

L

W

Y

H

E

D

A

T

L

E

E

G

G

K

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

A
x
G

M

S

G
|
G

T
|
T

T

G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

G

M

R

Y

Y

L

L

K

K

E

E

Q

R

N

I

P

P

D

H

V

V

Q

K

V

V

V

I

G

A

V

V

Q
x
E

P

P

T

A

E

R

G
x
S

S

N

S

V

I

L

P

S

-

G

-

K

-

M

-

G

-

Q

-

H

G

G

I

F

R

Q

R
x
G

W

M

P

G

K

P

E

G

Y

F

L

I

P

P

T

E

I

N

Y

L

D

D

D

L

S

S

R

L

V

L

D

D

R

G

T

V

I

I

D

Q

M

V

S

W

Q

E

S

E

L

D

A

A

E

F

D

P

T

L

M

A

I

R

R

R

M

L

A

A

K

R

E

E

G

G

I

L

F

F

A

L

G

G

V

M

S
|
S

S

S

G

G

A

I

M

V

A

W

A

A

L

L

Q

Q

I

V

A

A

N

R

E

E

V

L

D

G

-

P

N

G

A

K

L

R

I

V

V

A

T

C

I

I

V

S

C
x
P

D

D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K42), S70 (= S70), E200 (≠ Q200), S204 (≠ G204), S263 (= S256)
binding (3s)-3-hydroxybutanoic acid: K40 (= K42), G71 (= G71), T73 (= T73), Q141 (= Q141), G219 (≠ R212)
binding pyridoxal-5'-phosphate: K40 (= K42), N72 (= N72), Y172 (≠ S172), G173 (≠ A173), G175 (= G175), T176 (= T176), T179 (= T179), G219 (≠ R212), S263 (= S256), P289 (≠ C281)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
43% identity, 96% coverage: 8:293/297 of query aligns to 7:301/302 of 2ecoA

query
sites
2ecoA

V

I

G

G

N

K

T

T

P

P

L

V

I

V

K

R

V

L

Q

A

R

K

L

V

V

V

N

E

P

P

K

D

T

M

N

A

S

E

V

V

I

W

L

V

A

-

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

N

Y

M

M

I

I

K

K

Q

D

A

A

E

E

L

E

R

R

G

G

E

I

I

L

K

R

P

P

G

G

D

S

-

G

-

Q

T

V

L

I

I

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

V

I

A

A

M

S

L

R

G

G

Y

Y

K

R

M

L

K

I

L

L

I

T

M

M

P

P

D

A

N

Q

M

M

S

S

M

E

E

E

R

R

K

K

A

R

S

V

M

L

A

K

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

V

T

S

D

K

P

E

E

E

R

G

R

M

M

E

L

G

A

A

A

R

R

D

E

L

E

A

A

Q

L

K

R

M

L

Q

K

T

E

E

E

G

L

Q

G

G

A

M

F

V

M

L

P

D

D

Q
|
Q

F

F

A

K

N

N

P

P

D

A

N

N

P

V

L

R

S

A

H

H

Y

Y

F

E

T

T

G

G

P

P

E

E

I

L

W

Y

H

E

D

A

T

L

E

E

G

G

K

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

A

G

M

S

G
|
G

T
|
T

T

G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

G

M

R

Y

Y

L

L

K

K

E

E

Q

R

N

I

P

P

D

H

V

V

Q

K

V

V

V

I

G

A

V

V

Q
x
E

P

P

T

A

E

R

G
x
S

S

N

S

V

I

L

P

S

-

G

-

K

-

M

-

G

-

Q

-

H

G

G

I

F

R

Q

R
x
G

W

M

P

G

K

P

E

G

Y

F

L

I

P

P

T

E

I

N

Y

L

D

D

D

L

S

S

R

L

V

L

D

D

R

G

T

V

I

I

D

Q

M

V

S

W

Q

E

S

E

L

D

A

A

E

F

D

P

T

L

M

A

I

R

R

R

M

L

A

A

K

R

E

E

G

G

I

L

F

F

A

L

G

G

V

M

S
|
S

S

S

G

G

A

I

M

V

A

W

A

A

L

L

Q

Q

I

V

A

A

N

R

E

E

V

L

D

G

-

P

N

G

A

K

L

R

I

V

V

A

T

C

I

I

V

S

C
x
P

D
|
D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K42), S70 (= S70), E200 (≠ Q200), S204 (≠ G204), S263 (= S256)
binding 4-methyl valeric acid: K40 (= K42), T69 (= T69), G71 (= G71), T73 (= T73), Q141 (= Q141), G175 (= G175), T176 (= T176), G219 (≠ R212)
binding pyridoxal-5'-phosphate: K40 (= K42), N72 (= N72), Y172 (≠ S172), G175 (= G175), T176 (= T176), T179 (= T179), G219 (≠ R212), S263 (= S256), P289 (≠ C281), D290 (= D282)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
42% identity, 99% coverage: 2:294/297 of query aligns to 5:307/309 of 7n2tA

query
sites
7n2tA

K

K

T

D

L

V

M

T

D

E

V

L

V

I

G

G

N

N

T

T

P

P

L

L

I

V

K

Y

V

L

Q

N

R

R

L

V

V

V

N

D

P

-

K

G

T

C

N

V

S

A

V

R

I

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

Y

N

S

M

M

I

I

K

S

Q

D

A

A

E

E

L

N

R

K

G

G

E

L

I

I

K

T

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

A

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

V

I

A

A

M

A

L

K

G

G

Y

Y

K

R

M

L

K

I

L

I

I

C

M

M

P

P

D

A

N

S

M

M

S

S

M

L

E

E

R

R

K

R

A

T

S

I

M

L

A

R

A

A

Y

F

G

G

A

A

E

E

L

L

I

V

L

L

V

T

S

D

K

P

E

A

E

R

G

G

M

M

E

K

G

G

A

A

R

V

D

Q

L

K

A

A

Q

E

K

E

M

I

Q

K

T

A

E

K

G

T

-

P

Q

N

G

S

M

Y

V

I

L

L

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

L

K

S

I

H

H

Y

Y

F

E

T

T

G

G

P

P

E

E

I

I

W

W

H

R

D

G

T

S

E

G

G

G

K

K

I

I

T

D

H

A

F

L

V

V

S

S

A

G

M

I

G

G

T

T

T

G

G

G

T

T

I

V

M

T

G

G

T

A

S

G

M

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

P

P

D

N

V

I

Q

K

V

L

V

Y

G

G

V

V

Q

E

P

P

T

V

E

E

G

S

S

A

S

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

K

H

E

K

-

I

-

Q

-

G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

T

G

I

V

Y

L

D

D

D

V

S

N

R

L

V

L

D

D

R

E

T

V

I

I

D

Q

M

V

S

S

Q

S

S

E

L

E

A

S

E

I

D

E

T

T

M

A

I

K

R

L

M

L

A

A

K

L

E

K

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S

S

G

G

G

A

A

A

M

A

A

A

L

I

Q
x
R

I

I

A

A

N

K

E
x
R

V

P

D

E

N

N

A

A

-

G

-

K

L

L

I

I

V

V

T

A

I

V

V

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

D

E

binding phosphate ion: R277 (≠ Q266), R281 (≠ E270)

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
43% identity, 99% coverage: 2:294/297 of query aligns to 5:307/320 of 2isqA

query
sites
2isqA

K

K

T

D

L

V

M

T

D

E

V

L

V

I

G

G

N

N

T

T

P

P

L

L

I

V

K

Y

V

L

Q

N

R

N

L

V

V

A

N

E

P

G

K

C

T

V

N

G

S

R

V

V

I

A

L

-

A

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

N

S

M

M

I

I

K

S

Q

D

A

A

E

E

L

K

R

K

G

G

E

L

I

I

K

K

P

P

G

G

D

E

T

S

-

V

L

L

I

I

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

V

T

A

A

M

A

L

K

G

G

Y

Y

K

K

M

L

K

I

L

I

I

T

M

M

P

P

D

A

N

S

M

M

S

S

M

T

E

E

R

R

K

R

A

I

S

I

M

L

A

L

A

A

Y

F

G

G

A

V

E

E

L

L

I

V

L

L

V

T

S

D

K

P

E

A

E

K

G
|
G

M
|
M

E
x
K

G

G

A

A

R

I

D

A

L

K

A

A

Q

E

K

E

M

I

Q

L

T

A

E

K

G

T

-

P

Q

N

G

G

M

Y

V

M

L

L

D

Q

Q

Q

F

F

A

E

N

N

P

P

D

A

N

N

P

P

L

K

S

I

H

H

Y

Y

F

E

T

T

G

G

P

P

E

E

I

I

W

W

H

K

D

G

T

T

E

G

G

G

K

K

I

I

T

D

H

G

F

F

V

V

S

S

A
x
G

M

I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

A

S

G

M

K

Y

Y

L

L

K

K

E

E

Q

Q

N

N

P

A

D

N

V

V

Q

K

V

L

V

Y

G

G

V

V

Q

E

P

P

T

V

E

E

G

S

S

A

S

I

I

L

P

S

G

G

I

G

R

K

R

P

W
x
G

P
|
P

K
x
H

E

K

-

I

-
x
Q

-
x
G

-

I

-

G

-

A

-

G

Y

F

L

I

P

P

T

S

I

V

Y

L

D

N

D

V

S

D

R

L

V

I

D

D

R

E

T

V

I

V

D

Q

M

V

S

S

Q

S

S

D

L

E

A

S

E

I

D

D

T

M

M

A

I

R

R

Q

M

L

A

A

K

L

E

K

E

E

G

G

I

L

F

L

A

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

M

A

A

A

L

I

Q

K

I

L

A

A

N

Q

E

R

V

P

D

E

N

N

A

A

-

G

-

K

L

L

I

F

V

V

T

A

I

I

V

F

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

D

D

active site: K44 (= K42), S267 (= S256)
binding pyridoxal-5'-phosphate: K44 (= K42), N75 (= N72), G177 (≠ A173), G179 (= G175), T180 (= T176), G181 (≠ T177), T183 (= T179), G223 (vs. gap), S267 (= S256), P294 (≠ C281)
binding : T72 (= T69), S73 (= S70), G74 (= G71), T76 (= T73), G122 (= G119), M123 (= M120), K124 (≠ E121), G217 (≠ W213), P218 (= P214), H219 (≠ K215), Q222 (vs. gap), G223 (vs. gap)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
43% identity, 99% coverage: 2:294/297 of query aligns to 7:309/322 of P47998

query
sites
P47998

K

K

T

D

L

V

M

T

D

E

V

L

V

I

G

G

N

N

T

T

P

P

L

L

I

V

K

Y

V

L

Q

N

R

N

L

V

V

A

N

E

P

G

K

C

T

V

N

G

S

R

V

V

I

A

L

-

A

A

K

K

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K

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Q

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x
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K46 (= K42) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T69) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S70) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N72) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T73) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q141) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H151) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G156) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTTGT 175:179) binding pyridoxal 5'-phosphate
T182 (= T176) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T179) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ R211) mutation to A: Impaired interaction with SAT1.
H221 (≠ K215) mutation to A: Impaired interaction with SAT1.
K222 (≠ E216) mutation to A: Impaired interaction with SAT1.
S269 (= S256) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
43% identity, 96% coverage: 8:293/297 of query aligns to 12:312/323 of P0ABK5

query
sites
P0ABK5

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K42 (= K42) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
43% identity, 97% coverage: 6:294/297 of query aligns to 81:379/392 of P47999

query
sites
P47999

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