SitesBLAST
Comparing WP_211248857.1 NCBI__GCF_000621325.1:WP_211248857.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
41% identity, 94% coverage: 13:251/255 of query aligns to 3:241/244 of 1edoA
- active site: G12 (= G22), S138 (= S147), Y151 (= Y160), K155 (= K164)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G18), S10 (= S20), R11 (≠ S21), I13 (= I23), N31 (≠ G41), Y32 (vs. gap), A33 (= A42), R34 (= R43), S35 (≠ R44), D59 (= D68), V60 (= V69), N86 (= N95), A87 (= A96), S138 (= S147), Y151 (= Y160), K155 (= K164), P181 (= P190), G182 (= G191), I184 (≠ T193), S186 (≠ T195), M188 (= M197)
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
41% identity, 100% coverage: 2:255/255 of query aligns to 2:253/258 of 4wecA
- active site: G21 (= G22), S143 (= S147), Q154 (≠ A158), Y157 (= Y160), K161 (= K164)
- binding nicotinamide-adenine-dinucleotide: G17 (= G18), A19 (≠ S20), S20 (= S21), G21 (= G22), I22 (= I23), D41 (≠ A42), I42 (≠ R43), V61 (≠ T67), D62 (= D68), V63 (= V69), N89 (= N95), T141 (≠ N145), Y157 (= Y160), K161 (= K164), P187 (= P190), P189 (≠ I192), V190 (≠ T193)
7djsD Crystal structure of isopiperitenol dehydrogenase from pseudomonas aeruginosa complexed with NAD
43% identity, 96% coverage: 9:254/255 of query aligns to 3:250/251 of 7djsD
- binding nicotinamide-adenine-dinucleotide: G12 (= G18), G16 (= G22), I17 (= I23), D36 (≠ A42), L37 (≠ R43), C61 (≠ T67), D62 (= D68), V63 (= V69), N89 (= N95), A90 (= A96), T140 (≠ N145), S142 (= S147), Y155 (= Y160), K159 (= K164), A186 (≠ G191), V187 (≠ I192)
P40288 Glucose 1-dehydrogenase; EC 1.1.1.47 from Priestia megaterium (Bacillus megaterium) (see 2 papers)
38% identity, 97% coverage: 7:253/255 of query aligns to 3:251/261 of P40288
- 11:35 (vs. 15:39, 48% identical) binding NADP(+)
- E96 (= E99) mutation E->A,G,K: Heat stable.
- D108 (≠ E112) mutation to N: Heat stable.
- V112 (≠ T116) mutation to A: Heat stable.
- E133 (vs. gap) mutation to K: Heat stable.
- V183 (≠ I185) mutation to I: Heat stable.
- P194 (= P196) mutation to Q: Heat stable.
- E210 (≠ M212) mutation to K: Heat stable.
- Y217 (≠ R219) mutation to H: Heat stable.
Sites not aligning to the query:
- 252 Q→L: Heat stable.
- 253 Y→C: Heat stable.
- 258 A→G: Heat stable.
8y83A Crystal structure of a ketoreductase from sphingobacterium siyangense sy1 with co-enzyme
40% identity, 96% coverage: 9:254/255 of query aligns to 4:248/249 of 8y83A
- binding nicotinamide-adenine-dinucleotide: G13 (= G18), S16 (= S21), G17 (= G22), I18 (= I23), D37 (≠ A42), I38 (≠ R43), A62 (≠ T67), D63 (= D68), S64 (≠ V69), N90 (= N95), M141 (≠ N145), Y156 (= Y160), K160 (= K164), P186 (= P190), G187 (= G191), Y188 (≠ I192), I189 (≠ T193), L193 (≠ M197)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
38% identity, 97% coverage: 8:254/255 of query aligns to 4:254/255 of 5itvA
- active site: G18 (= G22), S141 (= S147), Y154 (= Y160), K158 (= K164)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G18), S17 (= S21), G18 (= G22), I19 (= I23), D38 (≠ A42), I39 (≠ R43), T61 (= T67), I63 (≠ V69), N89 (= N95), G91 (= G97), T139 (≠ N145), S141 (= S147), Y154 (= Y160), K158 (= K164), P184 (= P190), G185 (= G191), I186 (= I192), I187 (≠ T193)
1g6kA Crystal structure of glucose dehydrogenase mutant e96a complexed with NAD+
37% identity, 97% coverage: 7:253/255 of query aligns to 3:251/261 of 1g6kA
- active site: G18 (= G22), S145 (= S147), Y158 (= Y160), K162 (= K164)
- binding nicotinamide-adenine-dinucleotide: T17 (≠ S21), G18 (= G22), L19 (≠ I23), R39 (= R43), D65 (= D68), V66 (= V69), N92 (= N95), A93 (= A96), G94 (= G97), M143 (≠ N145), S145 (= S147), Y158 (= Y160), P188 (= P190), G189 (= G191), I191 (≠ T193), T193 (= T195)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
39% identity, 96% coverage: 12:255/255 of query aligns to 14:266/267 of Q9LBG2
- 17:42 (vs. 15:40, 50% identical) binding NAD(+)
- E103 (= E99) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
1iy8A Crystal structure of levodione reductase (see paper)
39% identity, 96% coverage: 12:255/255 of query aligns to 5:257/258 of 1iy8A
- active site: G15 (= G22), S143 (= S147), Q153 (≠ T157), Y156 (= Y160), K160 (= K164)
- binding nicotinamide-adenine-dinucleotide: G11 (= G18), S14 (= S21), G15 (= G22), L16 (≠ I23), D35 (≠ A42), V36 (≠ R43), A62 (vs. gap), D63 (= D68), V64 (= V69), N90 (= N95), G92 (= G97), I93 (≠ T98), T141 (≠ N145), S143 (= S147), Y156 (= Y160), K160 (= K164), P186 (= P190), G187 (= G191), T191 (= T195), P192 (= P196), M193 (= M197)
5jydB Crystal structure of a putative short chain dehydrogenase from burkholderia cenocepacia
39% identity, 96% coverage: 8:251/255 of query aligns to 44:288/292 of 5jydB
- active site: G58 (= G22), S184 (= S147), L194 (≠ T157), Y197 (= Y160), K201 (= K164), P242 (≠ E207)
- binding magnesium ion: D56 (≠ S20), S57 (= S21), E82 (= E46)
- binding nicotinamide-adenine-dinucleotide: G54 (= G18), D56 (≠ S20), S57 (= S21), G58 (= G22), I59 (= I23), L79 (≠ R43), E82 (= E46), D106 (= D68), I107 (≠ V69), N133 (= N95), A134 (= A96), A135 (≠ G97), T182 (≠ N145), S184 (= S147), Y197 (= Y160), K201 (= K164), P227 (= P190), G228 (= G191), P229 (≠ I192), Y230 (≠ T193), T232 (= T195), L234 (≠ M197), Q235 (≠ S198)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
36% identity, 96% coverage: 9:254/255 of query aligns to 3:247/248 of 6ixmC
- active site: G16 (= G22), S142 (= S147), Y155 (= Y160), K159 (= K164)
- binding nicotinamide-adenine-dinucleotide: G12 (= G18), S15 (= S21), G16 (= G22), I17 (= I23), D36 (≠ A42), I37 (≠ R43), A61 (≠ T67), D62 (= D68), T63 (≠ V69), N89 (= N95), A90 (= A96), M140 (≠ N145), S142 (= S147), Y155 (= Y160), K159 (= K164), P185 (= P190), A186 (≠ G191), Y187 (≠ I192), I188 (≠ T193), L192 (≠ M197)
5h5xC Crystal structure of nadh bound carbonyl reductase from streptomyces coelicolor
42% identity, 97% coverage: 8:254/255 of query aligns to 10:256/257 of 5h5xC
- active site: G24 (= G22), S151 (= S147), Y164 (= Y160), K168 (= K164)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G18), S23 (= S21), G24 (= G22), I25 (= I23), D44 (≠ A42), F45 (≠ R43), L69 (≠ T67), D70 (= D68), N97 (= N95), A98 (= A96), Y164 (= Y160), K168 (= K164), P194 (= P190), G195 (= G191), I197 (≠ T193), T199 (= T195)
3ay6B Crystal structure of bacillus megaterium glucose dehydrogenase 4 a258f mutant in complex with nadh and d-glucose (see paper)
38% identity, 96% coverage: 8:253/255 of query aligns to 10:257/267 of 3ay6B
- active site: G24 (= G22), S151 (= S147), Y164 (= Y160), K168 (= K164)
- binding beta-D-glucopyranose: E102 (= E99), S151 (= S147), H153 (≠ V149), W158 (≠ F154), Y164 (= Y160), N202 (≠ S198), K205 (≠ L201)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G18), T23 (≠ S21), G24 (= G22), L25 (≠ I23), Y45 (≠ R43), D71 (= D68), V72 (= V69), N98 (= N95), A99 (= A96), G100 (= G97), V101 (≠ T98), M149 (≠ N145), S151 (= S147), Y164 (= Y160), K168 (= K164), P194 (= P190), G195 (= G191), M197 (≠ T193), T199 (= T195), P200 (= P196), I201 (≠ M197), N202 (≠ S198)
8w0oA Gdh-105 crystal structure
37% identity, 96% coverage: 8:253/255 of query aligns to 4:251/259 of 8w0oA
- binding nicotinamide-adenine-dinucleotide: G14 (= G18), S17 (= S21), G18 (= G22), L19 (≠ I23), Y39 (vs. gap), Q43 (≠ A45), D65 (= D68), V66 (= V69), N92 (= N95), A93 (= A96), G94 (= G97), M143 (≠ N145), Y158 (= Y160), K162 (= K164), P188 (= P190), G189 (= G191), I191 (≠ T193), T193 (= T195)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
37% identity, 96% coverage: 8:251/255 of query aligns to 5:244/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G18), R18 (≠ S21), G19 (= G22), I20 (= I23), D39 (≠ A42), R40 (= R43), C63 (≠ T67), I65 (≠ V69), N91 (= N95), G93 (= G97), I94 (≠ T98), V114 (≠ T119), Y155 (= Y160), K159 (= K164), I188 (≠ T193), T190 (= T195), T193 (≠ S198)
7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
40% identity, 96% coverage: 8:251/255 of query aligns to 8:251/253 of 7v0hG
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G18), S20 (= S20), K21 (≠ S21), G22 (= G22), I23 (= I23), A43 (≠ R43), S44 (≠ R44), S45 (≠ A45), G68 (≠ T67), D69 (= D68), V70 (= V69), N96 (= N95), S97 (≠ A96), G98 (= G97), Y100 (≠ E99), I144 (≠ N145), S146 (= S147), Y159 (= Y160), K163 (= K164), P189 (= P190), G190 (= G191), M191 (≠ I192), I192 (≠ T193), T194 (= T195), G196 (≠ M197), T197 (≠ S198)
- binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S147), Y159 (= Y160), M191 (≠ I192), I202 (≠ L202)
4bmsF Short chain alcohol dehydrogenase from ralstonia sp. Dsm 6428 in complex with NADPH
39% identity, 96% coverage: 8:253/255 of query aligns to 3:247/249 of 4bmsF