SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_212636784.1 NCBI__GCF_900176285.1:WP_212636784.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P08237 ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase; EC 2.7.1.11 from Homo sapiens (Human) (see 6 papers)
43% identity, 98% coverage: 3:730/743 of query aligns to 16:754/780 of P08237

query
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P08237

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R39 (= R26) to P: in GSD7; Italian; dbSNP:rs121918193
G57 (= G44) to V: in GSD7; Italian
R100 (≠ Q87) to Q: in dbSNP:rs2228500
S180 (≠ T166) to C: in GSD7; Italian
G209 (= G195) to D: in GSD7; loss of activity shown by complementation assays in yeast; dbSNP:rs767265360
D309 (= D295) to G: in GSD7; Spanish; complete loss of enzyme activity; dbSNP:rs1169383137
D543 (= D530) to A: in GSD7; Italian; dbSNP:rs121918194
K557 (≠ R544) modified: N6-(2-hydroxyisobutyryl)lysine
D591 (≠ E577) to A: in GSD7; Italian
W686 (≠ E672) to C: in GSD7; Japanese; dbSNP:rs121918196
R696 (vs. gap) to H: in dbSNP:rs41291971

3o8lA Structure of phosphofructokinase from rabbit skeletal muscle (see paper)
42% identity, 98% coverage: 3:730/743 of query aligns to 8:746/748 of 3o8lA

query
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I

P

P

A

A

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T

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x
S

N

N

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|
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|
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I

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A

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active site: G17 (= G12), R80 (= R75), D111 (= D106), S156 (= S150), D158 (= D152), D160 (= D154), R202 (= R196)
binding adenosine-5'-diphosphate: D165 (= D159), M166 (= M160), F300 (= F294), F530 (= F525), F663 (= F657)
binding adenosine-5'-triphosphate: G16 (= G11), Y47 (= Y42), R80 (= R75), C81 (≠ S76), R85 (= R80), G110 (= G105), G112 (= G107), S113 (= S108), T115 (≠ M110), G116 (= G111), W219 (= W213), H234 (≠ K228), R238 (≠ A232), Y377 (≠ V371), H382 (≠ R376), I383 (= I377)
binding phosphate ion: R416 (= R410), G449 (≠ T443), G450 (= G444), R463 (= R457), S522 (≠ D517), N524 (= N519), R727 (≠ G711)

P00511 ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase; EC 2.7.1.11 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
42% identity, 98% coverage: 3:730/743 of query aligns to 16:754/780 of P00511

query
sites
P00511

R

K

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Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylthreonine
775 modified: Phosphoserine; by PKA

P47859 ATP-dependent 6-phosphofructokinase, platelet type; ATP-PFK; PFK-P; 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphohexokinase; EC 2.7.1.11 from Oryctolagus cuniculus (Rabbit) (see paper)
42% identity, 100% coverage: 3:742/743 of query aligns to 25:775/791 of P47859

query
sites
P47859

R

K

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A

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A

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A

A

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R

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M

G

G

V

V

A

E

A

A

V

V

E

L

L

A

L

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L

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A

A

G

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P

E

E

P

T

L

P

C

A

H

C

M

V

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S

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L

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S

K

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N

N

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H

V

A

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T

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P

L

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E

E

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C

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D

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A

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N

N

N

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L

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D

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V

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L

L

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E

S

K

F

M

R

K

C

T

G

T

K

I

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Q

M

R

V

G

I

L

Y

V

L

L

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N

N

E

E

N

S

A

C

S

S

R

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H

N

Y

Y

T

T

T

T

D

D

F

F

I

I

A

Y

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Q

L

L

L

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G

R

R

G

G

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A

N

I

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L

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G

H

H

L

M

Q

Q

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G

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A

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T

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A

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F

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D

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I

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L

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A

G

C

T

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K

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S

A

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A

A

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M

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Q

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W

M

I

G

T

R

T

L

K

L

L

E

K

E

E

G

S

A

P

R

G

D

K

G

G

-

K

-

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-

F

-

V

-

S

-

D

-

D

-

S

-

I

V

C

A

V

L

L

G

G

L

I

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K

R

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N

V

V

E

L

A

F

I

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P

P

L

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G

A

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A

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A

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D

D

L

F

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Sites not aligning to the query:

12 modified: Phosphoserine; by PKA

4xyjF Crystal structure of human phosphofructokinase-1 in complex with atp and mg, northeast structural genomics consortium target hr9275 (see paper)
42% identity, 100% coverage: 3:742/743 of query aligns to 11:756/761 of 4xyjF

query
sites
4xyjF

R

K

T

A

M

I

A

G

V

V

M

L

T

T

S

S

G
|
G

G
|
G

D

D

A

A

P

Q

G

G

M

M

N

N

A

A

G

A

V

V

R
|
R

A

A

V

V

V

V

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|
R

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A

G

L

I

D

Y

G

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G

G

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K

V

V

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Y

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G

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|
Y

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G

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G

G

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A

N

I

I

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W

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V

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x
S

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I

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L

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G

G

G

G

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T

F

I

L

I

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G

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A

A

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|
R

S
x
C

E

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A

F
|
F

R
|
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T

T

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R

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G

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R

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K

A

A

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N

L

L

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G

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I

I

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|
G

G
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G

D
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D

G
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S

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|
G

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A

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W

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|
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G

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|
G

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W

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I

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L

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|
E

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|
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-

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D

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L

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G

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P

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V

G

A

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A

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A

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D

D

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A

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R

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I

P

P

R

K

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W

F

W

L

L

E

K

L

L

D

R

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P

A

L

A

M

Q

K

I

I

L

L

A

A

R

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Y

A

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active site: G20 (= G12), R83 (= R75), C84 (≠ S76), D114 (= D106), G158 (= G149), S159 (= S150), D161 (= D152), D163 (= D154), R205 (= R196)
binding adenosine-5'-triphosphate: G20 (= G12), Y50 (= Y42), R83 (= R75), C84 (≠ S76), F87 (= F79), R88 (= R80), G113 (= G105), D114 (= D106), G115 (= G107), S116 (= S108), G119 (= G111), S159 (= S150)
binding cobalt (ii) ion: E475 (= E466), K510 (≠ S501), H511 (= H502)
binding magnesium ion: G19 (= G11), G20 (= G12), G112 (= G104), D114 (= D106), G158 (= G149), D163 (= D154), R205 (= R196)
binding phosphate ion: R30 (= R22), R34 (= R26), S69 (≠ G61), S70 (≠ G62), G219 (= G210), K250 (≠ R241), R416 (≠ S406), R420 (= R410), G453 (≠ T443), G454 (= G444)

4rh3A Amppcp-bound structure of human platelet phosphofructokinase in an r- state, crystal form ii (see paper)
42% identity, 98% coverage: 5:729/743 of query aligns to 3:731/731 of 4rh3A

query
sites
4rh3A

M

I

A

G

V

V

M

L

T

T

S

S

G
|
G

G
|
G

D

D

A

A

P

Q

G

G

M

M

N

N

A

A

G

A

V

V

R

R

A

A

V

V

V

V

R

R

R

M

A

G

L

I

D

Y

G

V

G

G

M

A

D

K

V

V

Y

Y

G

F

V

I

F

Y

S

E

G

G

Y

Y

E

Q

G

G

L

M

V

V

A

D

G

G

G

G

D

S

A

N

I

I

R

A

P

E

L

A

G

D

W

W

N

E

D

S

V

V

G

S

G

S

I

I

L

L

P

Q

R

V

G

G

G

G

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active site: G10 (= G12), R73 (= R75), C74 (≠ S76), D104 (= D106), G148 (= G149), S149 (= S150), D151 (= D152), D153 (= D154), R195 (= R196)
binding phosphomethylphosphonic acid adenylate ester: G9 (= G11), R73 (= R75), C74 (≠ S76), F77 (= F79), R78 (= R80), G103 (= G105), D104 (= D106), G105 (= G107), S106 (= S108), G109 (= G111)
binding phosphate ion: K240 (≠ R241), R406 (≠ S406), R410 (= R410), G443 (≠ T443), G444 (= G444), R457 (= R457), R552 (= R552), R641 (= R641), H647 (= H647)

4xyjA Crystal structure of human phosphofructokinase-1 in complex with atp and mg, northeast structural genomics consortium target hr9275 (see paper)
41% identity, 100% coverage: 3:742/743 of query aligns to 13:763/768 of 4xyjA

query
sites
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