SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_218017304.1 NCBI__GCF_001592305.1:WP_218017304.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
35% identity, 93% coverage: 35:461/461 of query aligns to 40:472/472 of P78061

query
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P78061

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H282 (= H272) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R347) mutation to Q: Activity is impaired to 3% of wild-type.

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
29% identity, 94% coverage: 22:453/461 of query aligns to 4:439/446 of 8ooqB

query
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8ooqB

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binding 2-oxoglutaric acid: F16 (≠ D34), R18 (≠ E36), A32 (≠ L50), R86 (≠ A105), V92 (≠ L111), P169 (≠ A193), R172 (≠ W196), R173 (≠ D197), S189 (≠ W213)
binding magnesium ion: E137 (= E153), E192 (= E216), E199 (= E223)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
29% identity, 94% coverage: 22:453/461 of query aligns to 5:440/447 of 8oooA

query
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8oooA

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F

D

A

L

M

A

S

-

E

-

A

-

E

-

K

-

E

-

L

-

G

-

I

R

E

G

S

L

V

P

P

R

A

T

N

F

L

S

K

T

A

A

A

H

L

D

D

Q

E

M

L

A

E

H

N

S

N

E

D

H

V

A

L

P

K

R

N

L

A

L

L

G

G

E

K

R

H

F

I

V

F

R

E

G

S

Y

F

L

L

A

E

V

I

K

K

A

N

L

A

E

E

H

W

D

D

H

S

Y

F

L

R

N

T

E

S

V

V

S

T

A

D

W

W

E

E

binding 2-oxoglutaric acid: F17 (≠ D34), R19 (≠ E36), A33 (≠ L50), R87 (≠ A105), V93 (≠ L111), P170 (≠ A193), R173 (≠ W196), R174 (≠ D197), S190 (≠ W213)
binding adenosine-5'-triphosphate: E136 (= E151), E188 (≠ D211), F203 (≠ L226), K204 (≠ L227), F205 (≠ H228), H251 (= H274), S253 (= S276), R325 (= R347), R335 (= R357)

8tfbA Glutamine synthetase (see paper)
32% identity, 81% coverage: 86:457/461 of query aligns to 64:440/443 of 8tfbA

query
sites
8tfbA

S

E

D

E

P

S

D

D

V

M

R

K

L

L

V

A

P

L

D

D

L

P

A

S

T

T

L

F

K

R

P

I

T

L

P

P

W

W

-

R

-

P

-

A

-

T

A

G

S

A

V

V

P

A

R

R

Y

I

L

L

A

G

V

-

H

-

D

D

C

V

F

Y

E

L

L

P

D

D

G

G

S

N

L

P

C

F

E

K

F

G

A

D

P

P

R

R

S

Y

V

V

L

L

K

K

Q

T

V

A

V

I

A

K

R

E

Y

A

R

E

A

K

R

M

G

G

L

F

T

S

P

M

V

N

I

V

A

G

P

P

E

E

I

L

E
|
E

F

F

Y

F

L

L

T

F

A

K

A

-

M

L

T

D

D

A

P

N

A

G

Q

N

P

P

L

T

A

T

Y

E

P

L

V

T

G

D

R

Q

G

G

R

-

P

-

E

-

V

-

G

-

Q

-

S

-

A

Y

F

F

S

D

M

F

N

A

L

P

L

L

N

D

E

R

L

A

A

Q

P

D

F

V

W

R

D

R

A

D

L

I

H

D

A

Y

A

A

I

L

D

E

G

H

L

M

G

G

I

F

R

Q

A

I

D

E

T

A

W

S

L

H

H

H

E
|
E

V

V

G

A

Q

P

S

S

Q

Q

Y

H

E
|
E

I

I

N

D

L

F

L

R

H

F

G

G

D

D

P

V

V

L

A

C

V

T

A

A

D

D

Q

N

A

V

F

V

L

T

F

F

K

K

T

Y

A

V

R

K

E

S

I

I

A

A

L

Y

Q

H

H

K

G

G

L

Y

N

Y

A

A

V

S

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

Q

V

A

N

G

G

S

S

S

G

M

M

H

H

L

S

H

N

Q

Q

S

S

V

L

V

F

D

-

A

K

Q

D

G

G

R

K

N

N

I

V

F

F

S

Y

Q

D

A

P

D

D

G

T

-

P

-

T

S

K

A

L

S

S

E

Q

A

D

F

A

L

M

H

Y

Y

Y

L

I

G

G

L

L

Q

L

A

K

Y

H

T

I

P

R

D

E

L

F

A

T

L

A

V

V

Y

T

A

N

P

P

S

V

V

V

N

N

S

S

Y

Y

R

K

R

R

Y

L

V

V

A

P

G

G

S

Y

Q

E

A

A

P

P

I

V

N

Y

V

I

A

S

W

W

G

S

H

A

D

Q

N

N

R

R

T

S

G

L

L

I

R

R

V

I

P

P

D

A

S

T

P

R

P

G

A

N

A

G

R

T

R

R

V

I

E

E

N

L

R

R

L

C

A

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

L

L

A

A

M

F

A

A

A

L

T

M

L

L

A

R

A

A

G

G

L

L

A

E

G

G

I

I

D

K

E

N

R

K

L

I

P

D

P

P

S

G

E

E

P

P

V

T

Q

N

G

V

N

N

G

I

Y

F

D

H

L

L

A

S

-

D

-

K

-

E

-

R

-

E

-

R

-

G

-

I

R

R

G

S

L

L

P

P

R

A

T

D

F

L

S

K

T

E

A

A

H

I

D

D

Q

E

M

M

A

K

H

G

S

S

E

K

H

F

A

V

P

K

R

E

L

A

L

L

G

G

E

E

R

H

F

V

V

F

R

S

G

H

Y

Y

L

L

A

C

V

A

K

K

A

E

L

M

E

E

H

W

D

D

H

E

Y

Y

L

K

N

A

E

V

V

V

S

H

A

P

W

W

E

E

-

L

R

S

R

R

Y

Y

L

L

binding magnesium ion: E133 (= E153), E188 (= E216), E195 (= E223)

8s59O Glutamine synthetase
32% identity, 81% coverage: 86:457/461 of query aligns to 67:443/446 of 8s59O

query
sites
8s59O

S

E

D

E

P

S

D

D

V

M

R

K

L

L

V

A

P

L

D

D

L

P

A

S

T

T

L

F

K

R

P

I

T

L

P

P

W

W

-
x
R

-

P

-

A

-

T

A

G

S

A

V
|
V

P

A

R

R

Y

I

L

L

A

G

V

-

H

-

D

D

C

V

F

Y

E

L

L

P

D

D

G

G

S

N

L

P

C

F

E

K

F

G

A

D

P

P

R

R

S

Y

V

V

L

L

K

K

Q

T

V

A

V

I

A

K

R

E

Y

A

R

E

A

K

R

M

G

G

L

F

T

S

P

M

V

N

I

V

A

G

P

P

E

E

I

L

E

E

F

F

Y

F

L

L

T

F

A

K

A

-

M

L

T

D

D

A

P

N

A

G

Q

N

P

P

L

T

A

T

Y

E

P

L

V

T

G

D

R

Q

G

G

R

-

P

-

E

-

V

-

G

-

Q

-

S

-

A

Y

F

F

S

D

M

F

N

A

L

P

L

L

N

D

E

R

L

A

A
x
Q

P

D

F

V

W
x
R

D
x
R

A

D

L

I

H

D

A

Y

A

A

I

L

D

E

G

H

L

M

G

G

I

F

R

Q

A

I

D

E

T

A

W
x
S

L

H

H

H

E

E

V

V

G

A

Q

P

S

S

Q

Q

Y

H

E

E

I

I

N

D

L

F

L

R

H

F

G

G

D

D

P

V

V

L

A

C

V

T

A

A

D

D

Q

N

A

V

F

V

L

T

F

F

K

K

T

Y

A

V

R

K

E

S

I

I

A

A

L

Y

Q

H

H

K

G

G

L

Y

N

Y

A

A

V

S

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

Q

V

A

N

G

G

S

S

S

G

M

M

H

H

L

S

H

N

Q

Q

S

S

V

L

V

F

D

-

A

K

Q

D

G

G

R

K

N

N

I

V

F

F

S

Y

Q

D

A

P

D

D

G

T

-

P

-

T

S

K

A

L

S

S

E

Q

A

D

F

A

L

M

H

Y

Y

Y

L

I

G

G

L

L

Q

L

A

K

Y

H

T

I

P

R

D

E

L

F

A

T

L

A

V

V

Y

T

A

N

P

P

S

V

V

V

N

N

S

S

Y

Y

R

K

R

R

Y

L

V

V

A

P

G

G

S

Y

Q

E

A

A

P

P

I

V

N

Y

V

I

A

S

W

W

G

S

H

A

D

Q

N

N

R

R

T

S

G

L

L

I

R

R

V

I

P

P

D

A

S

T

P

R

P

G

A

N

A

G

R

T

R

R

V

I

E

E

N

L

R

R

L

C

A

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

L

L

A

A

M

F

A

A

A

L

T

M

L

L

A

R

A

A

G

G

L

L

A

E

G

G

I

I

D

K

E

N

R

K

L

I

P

D

P

P

S

G

E

E

P

P

V

T

Q

N

G

V

N

N

G

I

Y

F

D

H

L

L

A

S

-

D

-

K

-

E

-

R

-

E

-

R

-

G

-

I

R

R

G

S

L

L

P

P

R

A

T

D

F

L

S

K

T

E

A

A

H

I

D

D

Q

E

M

M

A

K

H

G

S

S

E

K

H

F

A

V

P

K

R

E

L

A

L

L

G

G

E

E

R

H

F

V

V

F

R

S

G

H

Y

Y

L

L

A

C

V

A

K

K

A

E

L

M

E

E

H

W

D

D

H

E

Y

Y

L

K

N

A

E

V

V

V

S

H

A

P

W

W

E

E

-

L

R

S

R

R

Y

Y

L

L

binding 2-oxoglutaric acid: R86 (vs. gap), V92 (= V107), Q168 (≠ A193), R171 (≠ W196), R172 (≠ D197), S188 (≠ W213)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 23, 25, 39

8tfkA Glutamine synthetase (see paper)
32% identity, 81% coverage: 86:457/461 of query aligns to 61:437/440 of 8tfkA

query
sites
8tfkA

S

E

D

E

P

S

D

D

V

M

R

K

L

L

V

A

P

L

D

D

L

P

A

S

T

T

L

F

K

R

P

I

T

L

P

P

W

W

-

R

-

P

-

A

-

T

A

G

S

A

V

V

P

A

R

R

Y

I

L

L

A

G

V

-

H

-

D

D

C

V

F

Y

E

L

L

P

D

D

G

G

S

N

L

P

C

F

E

K

F

G

A

D

P

P

R

R

S

Y

V

V

L

L

K

K

Q

T

V

A

V

I

A

K

R

E

Y

A

R

E

A

K

R

M

G

G

L

F

T

S

P

M

V

N

I

V

A

G

P

P

E
|
E

I

L

E
|
E

F

F

Y

F

L

L

T

F

A

K

A

-

M

L

T

D

D

A

P

N

A

G

Q

N

P

P

L

T

A

T

Y

E

P

L

V

T

G

D

R

Q

G

G

R

-

P

-

E

-

V

-

G

-

Q

-

S

-

A

Y

F

F

S

D

M

F

N

A

L

P

L

L

N

D

E

R

L

A

A

Q

P

D

F

V

W

R

D

R

A

D

L

I

H

D

A

Y

A

A

I

L

D

E

G

H

L

M

G

G

I

F

R

Q

A

I

D

E

T

A

W

S

L

H

H

H

E
|
E

V

V

G

A

Q

P

S

S

Q

Q

Y

H

E
|
E

I

I

N
x
D

L
x
F

L

R

H
x
F

G

G

D

D

P

V

V

L

A

C

V

T

A

A

D

D

Q

N

A

V

F

V

L

T

F

F

K

K

T

Y

A

V

R

K

E

S

I

I

A

A

L

Y

Q

H

H

K

G

G

L

Y

N

Y

A

A

V

S

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

Q

V

A

N

G
|
G

S

S

S

G

M

M

H
|
H

L

S

H
x
N

Q

Q

S

S

V

L

V

F

D

-

A

K

Q

D

G

G

R

K

N

N

I

V

F

F

S

Y

Q

D

A

P

D

D

G

T

-

P

-

T

S

K

A

L

S

S

E

Q

A

D

F

A

L

M

H

Y

Y

Y

L

I

G

G

L

L

Q

L

A

K

Y

H

T

I

P

R

D

E

L

F

A

T

L

A

V

V

Y

T

A

N

P

P

S

V

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

A

P

G

G

S

Y

Q
x
E

A

A

P

P

I

V

N

Y

V

I

A

S

W

W

G

S

H

A

D

Q

N

N

R
|
R

T

S

G

L

L

I

R
|
R

V

I

P

P

D

A

S

T

P

R

P

G

A

N

A
x
G

R

T

R
|
R

V

I

E
|
E

N

L

R
|
R

L

C

A

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

L

L

A

A

M

F

A

A

A

L

T

M

L

L

A

R

A

A

G

G

L

L

A

E

G

G

I

I

D

K

E

N

R

K

L

I

P

D

P

P

S

G

E

E

P

P

V

T

Q

N

G

V

N

N

G

I

Y

F

D

H

L

L

A

S

-

D

-

K

-

E

-

R

-

E

-

R

-

G

-

I

R

R

G

S

L

L

P

P

R

A

T

D

F

L

S

K

T

E

A

A

H

I

D

D

Q

E

M

M

A

K

H

G

S

S

E

K

H

F

A

V

P

K

R

E

L

A

L

L

G

G

E

E

R

H

F

V

V

F

R

S

G

H

Y

Y

L

L

A

C

V

A

K

K

A

E

L

M

E

E

H

W

D

D

H

E

Y

Y

L

K

N

A

E

V

V

V

S

H

A

P

W

W

E

E

-

L

R

S

R

R

Y

Y

L

L

binding adenosine-5'-diphosphate: E128 (= E151), D194 (≠ N225), F195 (≠ L226), F197 (≠ H228), N243 (≠ H274), R312 (= R342), R317 (= R347), G325 (≠ A355), R327 (= R357)
binding magnesium ion: E128 (= E151), E128 (= E151), E130 (= E153), E185 (= E216), E192 (= E223), E192 (= E223), H241 (= H272), E329 (= E359)
binding l-methionine-s-sulfoximine phosphate: E128 (= E151), E130 (= E153), E185 (= E216), E192 (= E223), G237 (= G268), H241 (= H272), R294 (= R324), E300 (≠ Q330), R312 (= R342), R331 (= R361)

7tf6A Glutamine synthetase (see paper)
31% identity, 81% coverage: 86:460/461 of query aligns to 62:437/438 of 7tf6A

query
sites
7tf6A

S

E

D

E

P

S

D

D

V

M

R

Y

L

L

V

H

P

P

D

D

L

L

A

D

T

T

L

W

K

V

P

I

T

F

P

P

W

W

A

G

S

K

V

V

P

A

R

R

Y

L

L

I

A

C

V

-

H

-

D

D

C

V

F

Y

E

K

L

T

D

D

G

G

S

T

L

P

C

F

E

E

F

G

A

D

P

P

R

R

S

A

V

N

L

L

K

K

Q

R

V

V

V

L

A

K

R

E

Y

M

R

E

A

D

R

L

G

G

L

F

T

T

P

D

V

F

-

N

I

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

-

A

F

A

K

M

L

T

D

D

E

P

K

A

G

Q

E

P

P

L

T

A

L

Y

E

P

L

V

N

G

D

R

D

G

G

R

Y

P

F

E

D

V

L

G

A

Q

P

S

T

A

D

F

L

S

G

M

E

N

N

L

C

L

R

N

R

E

D

L

I

A

V

P

-

F

-

W

-

D

-

A

-

L

-

H

-

A

L

A

E

I

L

D

E

G

D

L

M

G

G

I

F

R

D

A

I

D

E

T

A

W

S

L

H

H

H

E
|
E

V

V

G

A

Q

P

S

G

Q

Q

Y

H

E
|
E

I

I

N

D

L

F

L

K

H

Y

G

A

D

D

P

A

V

V

A

T

V

A

A

C

D

D

Q

N

A

I

F

Q

L

T

F

F

K

K

T

L

A

V

R

K

E

T

I

I

A

A

L

R

Q

K

H

H

G

N

L

L

N

H

A

A

V

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G
|
G

Q

V

A
x
N

G
|
G

S

S

S

G

M

M

H
|
H

L

F

H

N

Q

V

S

S

V

L

V

F

D

K

A

G

Q

K

G

E

R

N

N

A

I

F

F

F

S

D

-

P

Q

N

A

T

D

E

G

M

S

G

A

L

S

T

E

E

A

T

F

A

L

Y

H

Q

Y

F

L

T

G

A

G

G

L

V

Q

L

A

K

Y

N

T

A

P

R

D

G

L

F

A

T

L

A

V

V

Y

C

A

N

P

P

S

L

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

A

P

G
|
G

S
x
Y

Q
x
E

A

A

P

P

I

C

N

Y

V

I

A

A

W

W

G

S

H

G

D

K

N

N

R
|
R

T

S

T

P

G

L

L

I

R

R

V

V

P

P

D

S

S

S

P

R

P

G

A

L

A

S

R

T

R

R

V

I

E
|
E

N

V

R

R

L

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

M

A

A

M

L

A

A

T

I

L

L

A

E

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

N

R

K

L

L

P

K

P

V

S

P

E

E

P

P

V

V

Q

N

G

Q

N

N

G

I

Y
|
Y

D

E

L

M

A

N

R

R

-

E

-

R

-

E

-

A

-

V

-

G

-

I

-

Q

G

D

L

L

P

P

R

S

T

T

F

L

S

Y

T

T

A

A

H

L

D

K

Q

A

M

M

A

R

H

E

S

N

E

E

H

V

A

I

P

K

R

K

L

A

L

L

G

G

E

N

R

H

F

I

V

Y

R

N

G

Q

Y

F

L

I

A

N

V

S

K

K

A

S

L

I

E

E

H

W

D

D

H
x
Y

Y

Y

L

R

N

T

E

Q

V

V

S

S

A

E

W

W

E

E

R

R

R

D

Y
x
Q

L

Y

L

M

P

K

Q
|
Q

binding glutamine: E128 (= E153), E183 (= E216), G235 (= G268), H239 (= H272), R292 (= R324), E298 (≠ Q330)
binding magnesium ion: E126 (= E151), E128 (= E153), E183 (= E216), E190 (= E223), H239 (= H272), E327 (= E359)
binding : G232 (= G265), N234 (≠ A267), G296 (= G328), Y297 (≠ S329), R310 (= R342), Y367 (= Y399), Y421 (≠ H444), Q433 (≠ Y456), Q437 (= Q460)

Sites not aligning to the query:

binding : 58, 60

4s0rD Structure of gs-tnra complex (see paper)
28% identity, 96% coverage: 20:460/461 of query aligns to 9:446/447 of 4s0rD

query
sites
4s0rD

R

R

A

E

Q

D

L

I

A

E

A

K

H

L

F

V

K

K

A

E

G

N

V

V

R

K

D

Y

V

I

E

R

C

L

L

Q

F

F

A

T

D

D

V

I

T

L

G

G

Y

T

P

I

R

K

G

N

K

V

L

E

M

I

P

P

A

V

A

S

S

-

F

-

A

-

G

-

E

-

L

-

R

Q

I

L

C

G

Q

K

A

A

I

L

P

D

M

N

Q

K

C

V

V

M

T

F

G
x
D

E

G

Y

S

S

S

Y

I

D

E

P

G

I
x
F

F
x
V

P
x
R

D
x
I

S

E

D

E

P

S

D

D

V

M

R

Y

L

L

V

Y

P

P

D

D

L

L

A

N

T

T

L

F

K

V

P

I

T

F

P

P

W

W

A

T

S

A

-

E

-

K

-

G

-

K

V

V

P

A

R

R

Y

F

L

I

A

C

V

-

H

-

D

D

C

I

F

Y

E

N

L

P

D

D

G

G

S

T

L

P

C

F

E

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

K

K

Q

R

V

I

V

L

A

K

R

E

Y

M

R

E

A

D

R

L

G

G

L

F

T

S

P

D

V

F

-

N

I

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

A

-

M

L

T

D

D

E

P

K

A

G

Q

E

P

P

L

T

A

L

Y

E

P

L

V

N

G

D

R

K

G

G

R

Y

P

F

E
x
D

V

L

G

A

Q

P

S

T

A

D

F

L

S

G

M

E

N

N

L

C

L

R

N

R

E

D

L

I

A

V

P

-

F

-

W

-

D

-

A

-

L

-

H

-

A

L

A

E

I

L

D

E

G

E

L

M

G

G

I

F

R

E

A

I

D

E

T

A

W

S

L

H

H

H

E
|
E

V

V

G

A

Q

P

S

G

Q

Q

Y

H

E
|
E

I

I

N

D

L

F

L

K

H

Y

G

A

D

G

P

A

V

V

A

R

V

S

A

C

D

D

Q

D

A

I

F

Q

L

T

F

F

K

K

T

L

A

V

R

K

E

T

I

I

A

A

L

R

Q

K

H

H

G

G

L

L

N

H

A

A

V

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G
|
G

Q
x
V

A
x
N

G

G

S

S

S

G

M

M

H
|
H

L

C

H

N

Q

L

S

S

V

L

V

F

D

-

A

K

Q

N

G

G

R

V

N

N

I

A

F

F

-

F

-

D

S

E

Q

N

A

A

D

D

G

L

S

Q

A

L

S

S

E

E

A

T

F

A

L

K

H

H

Y

F

L

I

G

A

G

G

L

I

Q

V

A

K

Y

H

T

A

P

T

D

S

L

F

A

T

L

A

V

V

Y

T

A

N

P

P

S

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

A

P

G
|
G

S
x
Y

Q
x
E

A

A

P

P

I

C

N

Y

V

V

A

A

W

W

G

S

H

A

D

Q

N

N

R
|
R

T

S

T

P

G

L

L

I

R

R

V

I

P

P

D

A

S

S

P

R

P

G

A

I

A

S

R

T

R

R

V

V

E
|
E

N

V

R
|
R

L

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

S

A

V

T

L

L

L

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

N

R

K

L

L

P

E

P

A

S

P

E

A

P

P

V

I

Q

D

G

R

N

N

G

I

Y
|
Y

D

V

L

M

A

S

R

K

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

G

D

L

L

P

P

R

A

T

T

F

L

S

A

T

E

A

A

H

L

D

E

Q

E

M

F

A

K

H

S

S

N

E

E

H

V

A

M

P

V

R

K

L

A

L

L

G

G

E

E

R

H

F

L

V

F

R

E

G
x
H

Y

F

L

I

A

E

V

A

K

K

A

E

L
x
I

E

E

H

W

D

D

H
x
M

Y

F

L

R

N

T

E

Q

V

V

S

H

A

P

W

W

E

E

R

R

R

E

Y

Q

L

Y

L

M

P

S

Q

Q

active site: D56 (≠ G75), E135 (= E151), E137 (= E153), E192 (= E216), E199 (= E223), H248 (= H272), R319 (= R342), E336 (= E359), R338 (= R361)
binding glutamine: E137 (= E153), E192 (= E216), R301 (= R324), E307 (≠ Q330)
binding magnesium ion: I66 (≠ D85), E135 (= E151), E135 (= E151), E199 (= E223), H248 (= H272), H248 (= H272), E336 (= E359), H419 (≠ G433)
binding : F63 (≠ I82), V64 (≠ F83), R65 (≠ P84), I66 (≠ D85), D161 (≠ E178), G241 (= G265), V242 (≠ Q266), N243 (≠ A267), G305 (= G328), Y306 (≠ S329), Y376 (= Y399), I426 (≠ L440), M430 (≠ H444)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
28% identity, 96% coverage: 20:460/461 of query aligns to 5:442/443 of 4lnkA

query
sites
4lnkA

R

R

A

E

Q

D

L

I

A

E

A

K

H

L

F

V

K

K

A

E

G

N

V

V

R

K

D

Y

V

I

E

R

C

L

L

Q

F

F

A

T

D

D

V

I

T

L

G

G

Y

T

P

I

R

K

G

N

K

V

L

E

M

I

P

P

A

V

A

S

S

-

F

-

A

-

G

-

E

-

L

-

R

Q

I

L

C

G

Q
x
K

A

A

I

L

P

D

M

N

Q

K

C

V

V
x
M

T

F

G
x
D

E

G

Y

S

S

S

Y

I

D

E

P

G

I

F

F

V

P

R

D

I

S

E

D

E

P

S

D

D

V

M

R

Y

L

L

V

Y

P

P

D

D

L

L

A

N

T

T

L

F

K

V

P

I

T

F

P

P

W

W

A

T

S

A

-

E

-

K

-

G

-

K

V

V

P

A

R

R

Y

F

L

I

A

C

V

-

H

-

D

D

C

I

F

Y

E

N

L

P

D

D

G

G

S

T

L

P

C

F

E

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

K

K

Q

R

V

I

V

L

A

K

R

E

Y

M

R

E

A

D

R

L

G

G

L

F

T

S

P

D

V

F

-

N

I

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

A

-

M

L

T

D

D

E

P

K

A

G

Q

E

P

P

L

T

A

L

Y

E

P

L

V

N

G

D

R

K

G

G

R

Y

P

F

E

D

V

L

G

A

Q

P

S

T

A

D

F

L

S

G

M

E

N

N

L

C

L

R

N

R

E

D

L

I

A

V

P

-

F

-

W

-

D

-

A

-

L

-

H

-

A

L

A

E

I

L

D

E

G

E

L

M

G

G

I

F

R

E

A

I

D

E

T

A

W

S

L

H

H

H

E
|
E

V
|
V

G

A

Q

P

S

G

Q

Q

Y

H

E
|
E

I

I

N

D

L
x
F

L

K

H
x
Y

G

A

D

G

P

A

V

V

A

R

V

S

A

C

D

D

Q

D

A

I

F

Q

L

T

F

F

K

K

T

L

A

V

R

K

E

T

I

I

A

A

L

R

Q

K

H

H

G

G

L

L

N

H

A

A

V

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

Q

V

A
x
N

G
|
G

S

S

S
x
G

M

M

H
|
H

L

C

H
x
N

Q

L

S
|
S

V

L

V

F

D

-

A

K

Q

N

G

G

R

V

N

N

I

A

F

F

-

F

-

D

S

E

Q

N

A

A

D

D

G

L

S

Q

A

L

S

S

E

E

A

T

F

A

L

K

H

H

Y

F

L

I

G

A

G

G

L

I

Q

V

A

K

Y

H

T

A

P

T

D

S

L

F

A

T

L

A

V

V

Y

T

A

N

P

P

S

T

V

V

N

N

S

S

Y

Y

R

K

R

R

Y

L

V

V

A

P

G

G

S

Y

Q
x
E

A

A

P

P

I

C

N

Y

V

V

A

A

W

W

G

S

H

A

D

Q

N

N

R
|
R

T

S

T

P

G

L

L

I

R

R

V

I

P

P

D

A

S
|
S

P

R

P

G

A

I

A
x
S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

L

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

S

A

V

T

L

L

L

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

N

R

K

L

L

P

E

P

A

S

P

E

A

P

P

V

I

Q

D

G

R

N

N

G

I

Y

Y

D

V

L

M

A

S

R

K

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

G

D

L

L

P

P

R

A

T

T

F

L

S

A

T

E

A

A

H

L

D

E

Q

E

M

F

A

K

H

S

S

N

E

E

H

V

A

M

P

V

R

K

L

A

L

L

G

G

E

E

R

H

F

L

V

F

R

E

G

H

Y

F

L

I

A

E

V

A

K

K

A

E

L

I

E

E

H

W

D

D

H

M

Y

F

L

R

N

T

E

Q

V

V

S

H

A

P

W

W

E

E

R

R

R

E

Y

Q

L

Y

L

M

P

S

Q

Q

active site: D52 (≠ G75), E131 (= E151), E133 (= E153), E188 (= E216), E195 (= E223), H244 (= H272), R315 (= R342), E332 (= E359), R334 (= R361)
binding adenosine-5'-diphosphate: K43 (≠ Q66), M50 (≠ V73), F198 (≠ L226), Y200 (≠ H228), N246 (≠ H274), S248 (= S276), S324 (= S351), S328 (≠ A355), R330 (= R357)
binding glutamic acid: E133 (= E153), E188 (= E216), V189 (= V217), N239 (≠ A267), G240 (= G268), G242 (≠ S270), E303 (≠ Q330)
binding magnesium ion: E131 (= E151), E188 (= E216), E195 (= E223), H244 (= H272), E332 (= E359)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
28% identity, 96% coverage: 20:460/461 of query aligns to 5:442/443 of 4lniA

query
sites
4lniA

R

R

A

E

Q

D

L

I

A

E

A

K

H

L

F

V

K

K

A

E

G

N

V

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Y

V

I

E

R

C

L

L

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F

F

A

T

D

D

V

I

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L

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G

Y

T

P

I

R

K

G

N

K

V

L

E

M

I

P

P

A

V

A

S

S

-

F

-

A

-

G

-

E

-

L

-

R

Q

I

L

C

G

Q

K

A

A

I

L

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D

M

N

Q

K

C

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V

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G
x
D

E

G

Y

S

S

S

Y

I

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G

I

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F

V

P

R

D

I

S

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D

E

P

S

D

D

V

M

R

Y

L

L

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Y

P

P

D

D

L

L

A

N

T

T

L

F

K

V

P

I

T

F

P

P

W

W

A

T

S

A

-

E

-

K

-

G

-

K

V

V

P

A

R

R

Y

F

L

I

A

C

V

-

H

-

D

D

C

I

F

Y

E

N

L

P

D

D

G

G

S

T

L

P

C

F

E

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

K

K

Q

R

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I

V

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A

K

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Y

M

R

E

A

D

R

L

G

G

L

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T

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D

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F

-

N

I

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

A

-

M

L

T

D

D

E

P

K

A

G

Q

E

P

P

L

T

A

L

Y

E

P

L

V

N

G

D

R

K

G

G

R

Y

P

F

E

D

V

L

G

A

Q

P

S

T

A

D

F

L

S

G

M

E

N

N

L

C

L

R

N

R

E

D

L

I

A

V

P

-

F

-

W

-

D

-

A

-

L

-

H

-

A

L

A

E

I

L

D

E

G

E

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M

G

G

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R

E

A

I

D
x
E

T

A

W

S

L

H

H

H

E
|
E

V

V

G

A

Q

P

S

G

Q

Q

Y

H

E
|
E

I

I

N
x
D

L

F

L

K

H
x
Y

G

A

D

G

P

A

V

V

A

R

V

S

A

C

D

D

Q

D

A

I

F

Q

L

T

F

F

K

K

T

L

A

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R

K

E

T

I

I

A

A

L

R

Q

K

H

H

G

G

L

L

N

H

A

A

V

T

F

F

M

M

A

P

K

K

P

P

I

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F

G

G

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V

A

N

G

G

S

S

S

G

M

M

H
|
H

L

C

H
x
N

Q

L

S
|
S

V

L

V

F

D

-

A

K

Q

N

G

G

R

V

N

N

I

A

F

F

-

F

-

D

S

E

Q

N

A

A

D

D

G

L

S

Q

A

L

S

S

E

E

A

T

F

A

L

K

H

H

Y

F

L

I

G

A

G

G

L

I

Q

V

A

K

Y

H

T

A

P

T

D

S

L

F

A

T

L

A

V

V

Y

T

A

N

P

P

S

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

A

P

G

G

S

Y

Q
x
E

A

A

P

P

I

C

N

Y

V

V

A

A

W

W

G

S

H

A

D

Q

N

N

R
|
R

T

S

T

P

G

L

L

I

R
|
R

V

I

P

P

D

A

S

S

P

R

P

G

A

I

A

S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

L

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

S

A

V

T

L

L

L

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

N

R

K

L

L

P

E

P

A

S

P

E

A

P

P

V

I

Q

D

G

R

N

N

G

I

Y

Y

D

V

L

M

A

S

R

K

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

G

D

L

L

P

P

R

A

T

T

F

L

S

A

T

E

A

A

H

L

D

E

Q

E

M

F

A

K

H

S

S

N

E

E

H

V

A

M

P

V

R

K

L

A

L

L

G

G

E

E

R

H

F

L

V

F

R

E

G

H

Y

F

L

I

A

E

V

A

K

K

A

E

L

I

E

E

H

W

D

D

H

M

Y

F

L

R

N

T

E

Q

V

V

S

H

A

P

W

W

E

E

R

R

R

E

Y

Q

L

Y

L

M

P

S

Q

Q

active site: D52 (≠ G75), E131 (= E151), E133 (= E153), E188 (= E216), E195 (= E223), H244 (= H272), R315 (= R342), E332 (= E359), R334 (= R361)
binding adenosine-5'-diphosphate: E131 (= E151), E183 (≠ D211), D197 (≠ N225), Y200 (≠ H228), N246 (≠ H274), S248 (= S276), R320 (= R347), R330 (= R357)
binding magnesium ion: E131 (= E151), E131 (= E151), E133 (= E153), E188 (= E216), E195 (= E223), E195 (= E223), H244 (= H272), E332 (= E359)
binding l-methionine-s-sulfoximine phosphate: E133 (= E153), E188 (= E216), H244 (= H272), R297 (= R324), E303 (≠ Q330), R315 (= R342), R334 (= R361)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
28% identity, 96% coverage: 20:460/461 of query aligns to 6:443/444 of P12425

query
sites
P12425

R

R

A

E

Q

D

L

I

A

E

A

K

H

L

F

V

K

K

A

E

G

N

V

V

R

K

D

Y

V

I

E

R

C

L

L

Q

F

F

A

T

D

D

V

I

T

L

G

G

Y

T

P

I

R

K

G

N

K

V

L

E

M

I

P

P

A

V

A

S

S

-

F

-

A

-

G

-

E

-

L

-

R

Q

I

L

C

G

Q

K

A

A

I

L

P

D

M

N

Q

K

C

V

V

M

T

F

G

D

E

G

Y

S

S

S

Y

I

D

E

P
x
G

I

F

F

V

P
x
R

D

I

S

E

D

E

P

S

D

D

V

M

R

Y

L

L

V

Y

P

P

D

D

L

L

A

N

T

T

L

F

K

V

P

I

T

F

P

P

W

W

A

T

S

A

-

E

-

K

-

G

-

K

V

V

P

A

R

R

Y

F

L

I

A

C

V

-

H

-

D

D

C

I

F

Y

E

N

L

P

D

D

G

G

S

T

L

P

C

F

E

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

K

K

Q

R

V

I

V

L

A

K

R

E

Y

M

R

E

A

D

R

L

G

G

L

F

T

S

P

D

V

F

-

N

I

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

F

A

K

A

-

M

L

T

D

D

E

P

K

A

G

Q

E

P

P

L

T

A

L

Y

E

P

L

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N

G

D

R

K

G

G

R

Y

P

F

E

D

V

L

G

A

Q

P

S

T

A

D

F

L

S

G

M

E

N

N

L

C

L

R

N

R

E

D

L

I

A

V

P

-

F

-

W

-

D

-

A

-

L

-

H

-

A

L

A

E

I

L

D

E

G

E

L

M

G

G

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F

R

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A

I

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T

A

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S

L

H

H

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E
|
E

V
|
V

G

A

Q

P

S

G

Q

Q

Y

H

E
|
E

I

I

N

D

L

F

L

K

H

Y

G

A

D

G

P

A

V

V

A

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V

S

A

C

D

D

Q

D

A

I

F

Q

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F

F

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K

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L

A

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K

E

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I

I

A

A

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K

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H

G

G

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L

N

H

A

A

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T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

Q

V

A

N

G
|
G

S

S

S

G

M

M

H
|
H

L

C

H

N

Q

L

S

S

V

L

V

F

D

-

A

K

Q

N

G

G

R

V

N

N

I

A

F

F

-

F

-

D

S

E

Q

N

A

A

D

D

G

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S

Q

A

L

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S

E

E

A

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F

A

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A

G

G

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A

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S

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F

A

T

L

A

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V

Y

T

A

N

P

P

S

T

V

V

N

N

S

S

Y

Y

R

K

R

R

Y

L

V

V

A

P

G
|
G

S

Y

Q
x
E

A

A

P
|
P

I

C

N

Y

V

V

A

A

W

W

G

S

H

A

D

Q

N

N

R

R

T

S

T

P

G

L

L

I

R

R

V

I

P

P

D

A

S

S

P

R

P

G

A

I

A

S

R

T

R

R

V

V

E
|
E

N

V

R

R

L

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

S

A

V

T

L

L

L

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

N

R

K

L

L

P

E

P

A

S

P

E

A

P

P

V

I

Q

D

G

R

N

N

G

I

Y

Y

D

V

L

M

A

S

R

K

-

E

-

R

-

M

-

E

-

N

-

G

-

I

-

V

G

D

L

L

P

P

R

A

T

T

F

L

S

A

T

E

A

A

H

L

D

E

Q

E

M

F

A

K

H

S

S

N

E

E

H

V

A

M

P

V

R

K

L

A

L

L

G

G

E

E

R

H

F

L

V

F

R

E

G

H

Y

F

L

I

A

E

V

A

K

K

A

E

L

I

E
|
E

H

W

D

D

H

M

Y

F

L

R

N

T

E

Q

V

V

S

H

A

P

W

W

E

E

R

R

R

E

Y

Q

L

Y

L

M

P

S

Q

Q

G59 (≠ P81) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (≠ P84) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E151) binding Mg(2+)
E134 (= E153) binding Mg(2+)
E189 (= E216) binding Mg(2+)
V190 (= V217) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E223) binding Mg(2+)
G241 (= G268) binding L-glutamate
H245 (= H272) binding Mg(2+)
G302 (= G328) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ Q330) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P332) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E359) binding Mg(2+)
E424 (= E441) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7tfaB Glutamine synthetase (see paper)
30% identity, 92% coverage: 31:454/461 of query aligns to 15:434/441 of 7tfaB

query
sites
7tfaB

G

N

V

V

R

R

D

F

V

I

E

R

C

L

L

Q

F

F

A

T

D

D

V

L

T

L

G

G

Y

T

P

I

R

K

G

N

K

V

L

E

M

I

P

P

A

V

A

S

S

Q

F

L

A

E

A

-

G

-

E

-

L

-

R

-

I

-

C

-

Q

K

A

A

I

L

P

D

M

N

Q

K

C

M

V

M

T

F

G

D

E

G

Y

S

S

S

Y

I

D

E

P

G

I
x
Y

F

V

P
x
R

D

I

S

E

D

E

P

S

D

D

V

M

R

Y

L

L

V

Y

P

P

D

D

L

L

A

D

T

T

L

W

K

V

P

V

T

F

P

P

W

W

A

V

S

T

V

S

P

D

R

R

Y

V

L

A

-

R

A

L

V

I

H

C

D

D

C

I

F

Y

E

K

L

P

D

D

G

G

S

S

L

P

C

F

E

A

F

G

A

D

P

P

R

R

S

G

V

I

L

L

K

K

Q

R

V

V

V

L

A

K

R

E

Y

A

R

E

A

E

R

L

G

G

L

Y

T

T

P

S

V

M

-

N

I

V

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

T

T

A

D

A

E

M

K

T

G

D

D

P

P

A

T

Q

T

P

E

L

L

A

N

Y

-

P

-

V

-

G

D

R

Q

G

G

R
x
Y

P

F

E

D

V

L

G

A

Q

P

S

M

A

D

F

L

S

G

M

E

N

N

L

C

L

R

N

R

E

E

L

I

A

V

P

-

F

-

W

-

D

-

A

-

L

-

H

-

A

L

A

K

I

L

D

E

G

E

L

M

G

G

I

F

R

E

A

I

D

E

T

A

W

S

L

H

H

H

E
|
E

V
|
V

G

A

Q

P

S

G

Q

Q

Y

H

E
|
E

I

I

N

D

L

F

L

K

H

Y

G

A

D

D

P

A

V

V

A

K

V

A

A

A

D

D

Q

Q

A

I

F

Q

L

T

F

F

K

K

T

L

A

V

R

K

E

T

I

I

A

A

L

R

Q

Q

H

H

G

G

L

L

N

H

A

A

V

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

Q

V

A
x
N

G
|
G

S

S

S

G

M

M

H
|
H

L

C

H

N

Q

Q

S

S

V

L

V

F

D

K

A

D

Q

N

G

E

R

N

N

V

I

F

F

Y

S

D

Q

E

A

T

D

D

G

E

-

L

S

G

A

L

S

S

E

Q

A

T

F

A

L

R

H

H

Y

Y

L

M

G

A

G

G

L

I

Q

L

A

K

Y

H

T

A

P

R

D

A

L

M

A

A

L

A

V

I

Y

T

A

N

P

P

S

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

A

P

G
|
G

S
x
Y

Q
x
E

A

A

P

P

I

C

N

Y

V

V

A

A

W

W

G

S

H

A

D

S

N

N

R
|
R

T

S

T

P

G

M

L

I

R

R

V

I

P

P

D

A

S

S

P

R

P

G

A

L

A

S

R

T

R

R

V

V

E
|
E

N

V

R

R

L

N

A

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

A

A

V

T

M

L

L

A

R

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

R

R

Q

L

M

P

A

P

L

S

P

E

A

P

P

V

I

Q

D

G

R

N

N

G

I

Y

Y

D

V

L

M

A

S

R

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

G

S

L

L

P

P

R

A

T

D

F

L

S

K

T

E

A

A

H

L

D

S

Q

E

M

L

A

I

H

R

S

S

E

E

H

V

A

I

P

S

R

D

L

A

L

L

G

G

E

D

R

H

F

A

V

L

R

A

G

Y

Y

F

L

Y

A

E

V

L

K

K

A

E

L

I

E

E

H

W

D

D

H
x
M

Y

Y

L

R

N

T

E

Q

V

V

S

H

A

Q

W

W

E

E

R

R

binding glutamine: E131 (= E153), Y153 (≠ R176), E186 (= E216), G238 (= G268), H242 (= H272), R295 (= R324), E301 (≠ Q330)
binding magnesium ion: E129 (= E151), E131 (= E153), E186 (= E216), E193 (= E223), H242 (= H272), E330 (= E359)
binding : Y58 (≠ I82), R60 (≠ P84), V187 (= V217), N237 (≠ A267), G299 (= G328), Y300 (≠ S329), R313 (= R342), M424 (≠ H444)

7tdvC Glutamine synthetase (see paper)
28% identity, 96% coverage: 20:460/461 of query aligns to 5:442/443 of 7tdvC

query
sites
7tdvC

R

K

A

E

Q

D

L

I

A

R

A

K

H

F

F

V

K

E

A

E

G

N

V

V

R

R

D

Y

V

L

E

R

C

L

L

Q

F

F

A

T

D

D

V

I

T

L

G

G

Y

T

P

I

R

K

G

N

K

V

L

E

M

V

P

P

A

V

A

S

S

-

F

-

A

-

G

-

E

-

L

-

R

Q

I

L

C

E

Q

K

A

V

I

L

P

D

M

N

Q

E

C

M

V

M

T

F

G

D

E

G

Y

S

S

S

Y

I

D

E

P

G

I

F

F

V

P

R

D

I

S

E

D

E

P

S

D

D

V

M

R

Y

L

L

V

H

P

P

D

D

L

L

A

D

T

T

L

W

K

V

P

I

T

F

P

P

W

W

A

T

S

A

V

G

P

Q

R

G

Y

K

L

V

A

A

-

R

-

L

V

I

H

C

D

D

C

V

F

Y

E

K

L

T

D

D

G

G

S

T

L

P

C

F

E

E

F

G

A

D

P

P

R

R

S

A

V

N

L

L

K

K

Q

R

V

V

V

L

A

K

R

E

Y

M

R

E

A

D

R

L

G

G

L

F

T

T

P

D

V

F

-

N

I

L

A
x
G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

-

A

F

A

K

M

L

T

D

D

E

P

K

A

G

Q

E

P

P

L

T

A

L

Y

E

P

L

V

N

G

D

R

D

G

G

R

Y

P

F

E

D

V

L

G

A

Q

P

S

T

A

D

F

L

S

G

M

E

N

N

L

C

L

R

N

R

E

D

L

I

A

V

P

-

F

-

W

-

D

-

A

-

L

-

H

-

A

L

A

E

I

L

D

E

G

D

L

M

G

G

I

F

R

D

A

I

D
x
E

T

A

W

S

L

H

H

H

E
|
E

V

V

G

A

Q

P

S

G

Q

Q

Y

H

E
|
E

I

I

N
x
D

L
x
F

L
x
K

H
x
Y

G

A

D

D

P

A

V

V

A

T

V

A

A

C

D

D

Q

N

A

I

F

Q

L

T

F

F

K

K

T

L

A

V

R

K

E

T

I

I

A

A

L

R

Q

K

H

H

G

N

L

L

N

H

A

A

V

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

Q

V

A

N

G
|
G

S

S

S

G

M

M

H
|
H

L

F

H
x
N

Q
x
V

S
|
S

V

L

V

F

D

K

A

G

Q

K

G

E

R

N

N

A

I

F

F

F

S

D

-

P

Q

N

A

T

D

E

G

M

S

G

A

L

S

T

E

E

A

T

F

A

L

Y

H

Q

Y

F

L

T

G

A

G

G

L

V

Q

L

A

K

Y

N

T

A

P

R

D

G

L

F

A

T

L

A

V

V

Y

C

A

N

P

P

S

L

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

A

P

G

G

S

Y

Q
x
E

A

A

P

P

I

C

N

Y

V

I

A

A

W

W

G

S

H

G

D

K

N

N

R
|
R

T

S

T

P

G

L

L

I

R
|
R

V

V

P

P

D

S

S

S

P

R

P

G

A

L

A
x
S

R

T

R
|
R

V

I

E
|
E

N

V

R

R

L

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

M

A

A

M

L

A

A

T

I

L

L

A

E

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

N

R

K

L

L

P

K

P

V

S

P

E

E

P

P

V

V

Q

N

G

Q

N

N

G

I

Y

Y

D

E

L

M

A

N

R

R

-

E

-

R

-

E

-

A

-

V

-

G

-

I

-

Q

G

D

L

L

P

P

R

S

T

T

F

L

S

Y

T

T

A

A

H

L

D

K

Q

A

M

M

A

R

H

E

S

N

E

E

H

V

A

I

P

K

R

K

L

A

L

L

G

G

E

N

R

H

F

I

V

Y

R

N

G

Q

Y

F

L

I

A

N

V

S

K

K

A

S

L

I

E

E

H

W

D

D

H

Y

Y

Y

L

R

N

T

E

Q

V

V

S

S

A

E

W

W

E

E

R

R

R

D

Y

Q

L

Y

L

M

P

K

Q

Q

binding adenosine-5'-diphosphate: G129 (≠ A149), E131 (= E151), E183 (≠ D211), D197 (≠ N225), F198 (≠ L226), K199 (≠ L227), Y200 (≠ H228), N246 (≠ H274), V247 (≠ Q275), S248 (= S276), R320 (= R347), S328 (≠ A355), R330 (= R357)
binding magnesium ion: E131 (= E151), E131 (= E151), E133 (= E153), E188 (= E216), E195 (= E223), E195 (= E223), H244 (= H272), E332 (= E359)
binding l-methionine-s-sulfoximine phosphate: E131 (= E151), E133 (= E153), E188 (= E216), E195 (= E223), G240 (= G268), H244 (= H272), R297 (= R324), E303 (≠ Q330), R315 (= R342)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
30% identity, 92% coverage: 31:454/461 of query aligns to 15:432/439 of 7tdpA

query
sites
7tdpA

G

N

V

V

R

R

D

F

V

I

E

R

C

L

L

Q

F

F

A

T

D

D

V

L

T

L

G

G

Y

T

P

I

R

K

G

N

K

V

L

E

M

I

P

P

A

V

A

S

S

Q

F

L

A

E

A

-

G

-

E

-

L

-

R

-

I

-

C

-

Q

K

A

A

I

L

P

D

M

N

Q

K

C

M

V

M

T

F

G

D

E

G

Y

S

S

S

Y

I

D

E

P

G

I

Y

F

V

P

R

D

I

S

E

D

E

P

S

D

D

V

M

R

Y

L

L

V

Y

P

P

D

D

L

L

A

D

T

T

L

W

K

V

P

V

T

F

P

P

W

W

A

S

S

D

-

R

V

V

P

A

R

R

Y

L

L

I

A

C

V

-

H

-

D

D

C

I

F

Y

E

K

L

P

D

D

G

G

S

S

L

P

C

F

E

A

F

G

A

D

P

P

R

R

S

G

V

I

L

L

K

K

Q

R

V

V

V

L

A

K

R

E

Y

A

R

E

A

E

R

L

G

G

L

Y

T

T

P

S

V

M

-
x
N

I

V

A
x
G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

T

T

A

D

A

E

M

K

T

G

D

D

P

P

A

T

Q

T

P

E

L

L

A

N

Y

-

P

-

V

-

G

D

R

Q

G

G

R

Y

P

F

E

D

V

L

G

A

Q

P

S

M

A

D

F

L

S

G

M

E

N

N

L

C

L

R

N

R

E

E

L

I

A

V

P

-

F

-

W

-

D

-

A

-

L

-

H

-

A

L

A

K

I

L

D

E

G

E

L

M

G

G

I

F

R

E

A

I

D
x
E

T

A

W

S

L

H

H

H

E
|
E

V

V

G

A

Q

P

S

G

Q

Q

Y

H

E
|
E

I

I

N
x
D

L

F

L

K

H
x
Y

G

A

D

D

P

A

V

V

A

K

V

A

A

A

D

D

Q

Q

A

I

F

Q

L

T

F

F

K

K

T

L

A

V

R

K

E

T

I

I

A

A

L

R

Q

Q

H

H

G

G

L

L

N

H

A

A

V

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

Q

V

A

N

G
|
G

S

S

S

G

M

M

H
|
H

L

C

H
x
N

Q

Q

S
|
S

V

L

V

F

D

K

A

D

Q

N

G

E

R

N

N

V

I

F

F

Y

S

D

Q

E

A

T

D

D

G

E

-

L

S

G

A

L

S

S

E

Q

A

T

F

A

L

R

H

H

Y

Y

L

M

G

A

G

G

L

I

Q

L

A

K

Y

H

T

A

P

R

D

A

L

M

A

A

L

A

V

I

Y

T

A

N

P

P

S

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

A

P

G

G

S

Y

Q
x
E

A

A

P

P

I

C

N

Y

V

V

A

A

W

W

G

S

H

A

D

S

N

N

R
|
R

T

S

T

P

G

M

L

I

R
|
R

V

I

P

P

D

A

S

S

P

R

P

G

A

L

A

S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

L

N

A

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

M

L

A

A

A

V

T

M

L

L

A

R

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

R

R

Q

L

M

P

A

P

L

S

P

E

A

P

P

V

I

Q

D

G

R

N

N

G

I

Y

Y

D

V

L

M

A

S

R

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

G

S

L

L

P

P

R

A

T

D

F

L

S

K

T

E

A

A

H

L

D

S

Q

E

M

L

A

I

H

R

S

S

E

E

H

V

A

I

P

S

R

D

L

A

L

L

G

G

E

D

R

H

F

A

V

L

R

A

G

Y

Y

F

L

Y

A

E

V

L

K

K

A

E

L

I

E

E

H

W

D

D

H

M

Y

Y

L

R

N

T

E

Q

V

V

S

H

A

Q

W

W

E

E

R

R

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A149), E127 (= E151), E179 (≠ D211), D193 (≠ N225), Y196 (≠ H228), N242 (≠ H274), S244 (= S276), R316 (= R347), R326 (= R357)
binding magnesium ion: E127 (= E151), E127 (= E151), E129 (= E153), E184 (= E216), E191 (= E223), E191 (= E223), H240 (= H272), E328 (= E359)
binding l-methionine-s-sulfoximine phosphate: E127 (= E151), E129 (= E153), E184 (= E216), E191 (= E223), G236 (= G268), H240 (= H272), R293 (= R324), E299 (≠ Q330), R311 (= R342), R330 (= R361)

7tenA Glutamine synthetase (see paper)
28% identity, 93% coverage: 31:458/461 of query aligns to 15:439/442 of 7tenA

query
sites
7tenA

G

N

V

V

R

K

D

F

V

I

E

R

C

L

L

Q

F

F

A

T

D

D

V

I

T

L

G

G

Y

I

P

I

R

K

G

N

K

V

L

E

M

I

P

P

A

V

A

S

S

-

F

-

A

-

G

-

E

-

L

-

R

Q

I

L

C

K

Q

K

A

A

I

L

P

D

M

N

Q

K

C

I

V

M

T

F

G

D

E

G

Y

S

S

S

Y

I

D

E

P

G

I

F

F

V

P

R

D

I

S

E

D

E

P

S

D

D

V

M

R

Y

L

L

V

F

P

P

D

D

L

L

A

D

T

T

L

W

K

V

P

V

T

F

P

P

W

W

A

T

S

A

V

E

P

K

R

G

Y

K

L

V

A

A

V

R

H

M

D

I

C

C

F

D

E

I

L

Y

D

N

G

P

S

D

L

M

C

T

E

P

F

F

A

A

-

G

-

D

P

P

R

R

S

A

V

N

L

L

K

K

Q

R

V

V

V

L

A

K

R

E

Y

M

R

E

A

E

R

L

G

G

L

F

T

T

P

E

V

F

-

N

I

L

A
x
G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

-

A

F

A

K

M

L

T

D

D

E

P

N

A

R

Q

R

P

P

L

T

A

L

Y

E

P

L

V

N

G

D

R

S

G

G

R

Y

P

F

E

D

V

L

G

A

Q

P

S

T

A

D

F

L

S

G

M

E

N

N

L

C

L

R

N

R

E

D

L

I

A

V

P

-

F

-

W

-

D

-

A

-

L

-

H

-

A

L

A

E

I

L

D

E

G

E

L

M

G

G

I

F

R

E

A

I

D
x
E

T

A

W

S

L

H

H

H

E
|
E

V

V

G

A

Q

P

S

G

Q

Q

Y

H

E
|
E

I

I

N
x
D

L
x
F

L
x
K

H
x
Y

G

E

D

D

P

A

V

I

A

T

V

A

A

C

D

D

Q

S

A

I

F

Q

L

T

F

F

K

K

T

L

A

V

R

K

E

T

I

I

A

A

L

R

Q

K

H

H

G

G

L

L

N

H

A

A

V

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

Q

V

A
x
N

G
|
G

S

S

S

G

M

M

H
|
H

L

F

H
x
N

Q

M

S
|
S

V

L

V

F

D

N

A

E

Q

K

G

G

R

N

N

A

I

F

F

F

S

D

Q

E

A

S

-

G

D

E

G

L

S

E

A

L

S

S

E

Q

A

T

F

A

L

Y

H

H

Y

F

L

L

G

A

G

G

L

M

Q

L

A

K

Y

H

T

A

P

R

D

G

L

Y

A

T

L

A

V

V

Y

T

A

N

P

P

S

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

Y

L

V

V

A

P

G

G

S

Y

Q
x
E

A

A

P

P

I

C

N

Y

V

I

A

A

W

W

G

S

H

G

D

K

N

N

R
|
R

T

S

T

P

G

L

L

V

R
|
R

V

V

P

P

D

S

S

S

P

R

P

G

A

L

A
x
S

R

T

R
|
R

V

L

E

E

N

L

R
|
R

L

S

A

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

L

L

A

A

M

M

A

A

A

V

T

L

L

L

A

K

A

A

G

G

L

L

A

S

G

G

I

I

D

K

E

D

R

E

L

L

P

T

P

P

S

P

E

A

P

P

V

V

Q

D

G

R

N

N

G

I

Y

Y

D

G

L

M

A

N

R

E

-

E

-

R

-

E

-

A

-

T

-

G

-

I

-

Y

G

D

L

L

P

P

R

E

T

S

F

L

S

G

T

H

A

A

H

L

D

I

Q

E

M

L

A

E

H

K

S

N

E

E

H

I

A

I

P

K

R

D

L

G

L

L

G

G

E

E

R

H

F

I

V

F

R

E

G

H

Y

F

L

I

A

E

V

A

K

K

A

T

L

I

E

E

H

C

D

D

H

M

Y

F

L

R

N

T

E

A

V

V

S

H

A

P

W

W

E

E

R

R

R

E

Y

Q

L

Y

L

L

binding adenosine-5'-diphosphate: G128 (≠ A149), E130 (= E151), E182 (≠ D211), D196 (≠ N225), F197 (≠ L226), K198 (≠ L227), Y199 (≠ H228), N245 (≠ H274), S247 (= S276), R319 (= R347), S327 (≠ A355), R329 (= R357)
binding l-methionine-s-sulfoximine phosphate: E130 (= E151), E132 (= E153), E187 (= E216), E194 (= E223), N238 (≠ A267), G239 (= G268), H243 (= H272), R296 (= R324), E302 (≠ Q330), R314 (= R342), R333 (= R361)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
28% identity, 93% coverage: 31:458/461 of query aligns to 16:440/443 of 7tf9S

query
sites
7tf9S

G

N

V

V

R

K

D

F

V

I

E

R

C

L

L

Q

F

F

A

T

D

D

V

I

T

L

G

G

Y

I

P

I

R

K

G

N

K

V

L

E

M

I

P

P

A

V

A

S

S

-

F

-

A

-

G

-

E

-

L

-

R

Q

I

L

C

K

Q

K

A

A

I

L

P

D

M

N

Q

K

C

I

V

M

T

F

G

D

E

G

Y

S

S

S

Y

I

D

E

P

G

I
x
F

F
x
V

P

R

D

I

S

E

D

E

P

S

D

D

V

M

R

Y

L

L

V

F

P

P

D

D

L

L

A

D

T

T

L

W

K

V

P

V

T

F

P

P

W

W

A

T

S

A

V

E

P

K

R

G

Y

K

L

V

A

A

V

R

H

M

D

I

C

C

F

D

E

I

L

Y

D

N

G

P

S

D

L

M

C

T

E

P

F

F

A

A

-

G

-

D

P

P

R

R

S

A

V

N

L

L

K

K

Q

R

V

V

V

L

A

K

R

E

Y

M

R

E

A

E

R

L

G

G

L

F

T

T

P

E

V

F

-

N

I

L

A

G

P

P

E
|
E

I

P

E
|
E

F

F

Y

F

L

L

T

-

A

F

A

K

M

L

T

D

D

E

P

N

A

R

Q

R

P

P

L

T

A

L

Y

E

P

L

V

N

G

D

R

S

G

G

R
x
Y

P

F

E

D

V

L

G

A

Q

P

S

T

A

D

F

L

S

G

M

E

N

N

L

C

L

R

N

R

E

D

L

I

A

V

P

-

F

-

W

-

D

-

A

-

L

-

H

-

A

L

A

E

I

L

D

E

G

E

L

M

G

G

I

F

R

E

A

I

D

E

T

A

W

S

L

H

H

H

E
|
E

V

V

G

A

Q

P

S

G

Q

Q

Y

H

E
|
E

I

I

N

D

L

F

L

K

H

Y

G

E

D

D

P

A

V

I

A

T

V

A

A

C

D

D

Q

S

A

I

F

Q

L

T

F

F

K

K

T

L

A

V

R

K

E

T

I

I

A

A

L

R

Q

K

H

H

G

G

L

L

N

H

A

A

V

T

F

F

M

M

A

P

K

K

P

P

I

L

S

F

G

G

Q

V

A

N

G
|
G

S

S

S
x
G

M

M

H
|
H

L

F

H

N

Q

M

S

S

V

L

V

F

D

N

A

E

Q

K

G

G

R

N

N

A

I

F

F

F

S

D

Q

E

A

S

-

G

D

E

G

L

S

E

A

L

S

S

E

Q

A

T

F

A

L

Y

H

H

Y

F

L

L

G

A

G

G

L

M

Q

L

A

K

Y

H

T

A

P

R

D

G

L

Y

A

T

L

A

V

V

Y

T

A

N

P

P

S

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

Y

L

V

V

A

P

G

G

S

Y

Q
x
E

A

A

P

P

I

C

N

Y

V

I

A

A

W

W

G

S

H

G

D

K

N

N

R

R

T

S

T

P

G

L

L

V

R

R

V

V

P

P

D

S

S

S

P

R

P

G

A

L

A

S

R

T

R

R

V

L

E
|
E

N

L

R

R

L

S

A

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

L

L

A

A

M

M

A

A

A

V

T

L

L

L

A

K

A

A

G

G

L

L

A

S

G

G

I

I

D

K

E

D

R

E

L

L

P

T

P

P

S

P

E

A

P

P

V

V

Q

D

G

R

N

N

G

I

Y

Y

D

G

L

M

A

N

R

E

-

E

-

R

-

E

-

A

-

T

-

G

-

I

-

Y

G

D

L

L

P

P

R

E

T

S

F

L

S

G

T

H

A

A

H

L

D

I

Q

E

M

L

A

E

H

K

S

N

E

E

H

I

A

I

P

K

R

D

L

G

L

L

G

G

E

E

R

H

F

I

V

F

R

E

G

H

Y

F

L

I

A
x
E

V

A

K

K

A

T

L
x
I

E

E

H

C

D

D

H
x
M

Y

F

L

R

N

T

E

A

V

V

S

H

A

P

W

W

E

E

R

R

R

E

Y

Q

L

Y

L

L

binding glutamine: E133 (= E153), Y155 (≠ R176), E188 (= E216), G240 (= G268), G242 (≠ S270), R297 (= R324), E303 (≠ Q330)
binding magnesium ion: E131 (= E151), E133 (= E153), E188 (= E216), E195 (= E223), H244 (= H272), E332 (= E359)
binding : F59 (≠ I82), V60 (≠ F83), E418 (≠ A436), I422 (≠ L440), M426 (≠ H444)

8oozA Glutamine synthetase (see paper)
30% identity, 93% coverage: 28:457/461 of query aligns to 11:427/430 of 8oozA

query
sites
8oozA

K

E

A

E

A

K

G

N

V

V

R

R

D

F

V

V

E

R

C

L

L

Q

F

F

A

V

D

D

V

V

T

Q

G

G

Y

I

P

P

R

K

G

N

K

V

L

A

M

I

P

P

A

V

A

G

S

Q

F

L

A

-

G

-

E

E

L

K

R

A

I

L

C

G

Q

P

A

G

I

I

P

H

M

F

Q

D

C

G

V

S

T

S

G

I

E

E

Y

G

S

S

Y

-

D

-

P

-

I

-

F

-

P

-

D

-

S

-

D

-

P

-

D

D

V

M

R

V

L

L

V

R

P

P

D

D

L

P

A

D

T

T

L

F

K

R

P

V

T

L

P

P

W

W

A

S

S

T

V

A

P

E

R

A

Y

R

L

L

A

-

V

I

H

C

D

D

C

I

F

E

E

L

L

P

D

D

G

G

S

K

L

P

C

F

E

M

F

G

A

C

P

P

R

R

S

Q

V

V

L

L

K

K

Q

K

V

N

V

M

A

E

R

E

Y

A

R

A

A

K

R

L

G

G

L

Y

T

V

P

M

V

N

I

T

A
x
G

P

P

E
|
E

I

M

E

E

F

F

Y

F

L

L

T

F

A

K

A

R

M

Q

T

D

D

-

P

-

A

G

Q

M

P

P

L

T

A

N

Y

I

P

P

V

Q

G

D

R

R

G

G

R

Y

P

F

E

D

V

L

G

A

Q

P

S

I

A

D

F

L

S

A

M

E

N

E

L

I

L

K

N

R

E

E

L

I

A

V

P

-

F

-

W

-

D

-

A

-

L

-

H

-

A

L

A

V

I

L

D

E

G

E

L

M

G

G

I

F

R

E

A

V

D
x
E

T

A

W

A

L

H

H

H

E

E

V

V

G

A

Q

F

S

G

Q

Q

Y

H

E

E

I

I

N

D

L
x
F

L
x
K

H
x
Y

G

D

D

N

P

A

V

L

A

A

V

T

A

A

D

D

Q

N

A

V

F

I

L

T

F

L

K

K

T

Y

A

V

A

A

R

K

E

T

I

L

A

A

L

L

Q

Q

H

H

G

G

L

L

N

H

A

A

V

T

F

F

M

M

A

P

K

K

P

P

I

I

S

F

G

G

Q

V

A

N

G

G

S

S

S

G

M

M

H
|
H

L

T

H
x
N

Q

T

S
|
S

V

L

V

F

D

-

A

K

Q

D

G

G

R

K

N

N

I

A

F

F

S

Y

Q

D

A

P

D

D

G

A

-

P

-

D

S

Q

A

I

S

S

E

D

A

T

F

L

L

R

H

Y

Y

F

L

V

G

G

L

V

Q

L

A

K

Y

H

T

I

P

R

D

A

L

I

A

T

L

A

V

I

Y

T

A

N

P

P

S

L

V

V

N

N

S

S

Y

Y

R

K

R

R

Y

L

V

V

A

P

G

G

S

Y

Q

E

A

A

P

P

I

V

N

Y

V

I

A

T

W

W

G

S

H

G

D

P

N

N

R

R

T

S

G

L

L

I

R

R

V

V

P

P

D

A

S

P

P

R

P

G

A

N

A
x
S

R

T

R
|
R

V

I

E
|
E

N

I

R

R

L

S

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

A

A

M

F

A

A

T

I

L

L

A

A

G

G

L

L

A

D

G

G

I

V

D

K

E

N

R

K

L

I

P

E

P

P

S

P

E

E

P

R

V

V

Q

E

G

K

N

N

G

I

Y

Y

D

K

L

L

A

T

R

E

G

E

-

E

-

R

-

E

-

K

-

L

-

G

-

I

-

G

-

M

L

L

P

P

R

G

T

T

F

L

S

K

T

E

A

A

H

I

D

E

Q

C

M

F

A

K

H

E

S

D

E

E

H

L

A

L

P

V

R

S

L

A

L

L

G

G

E

E

R

H

F

V

V

S

R

Q

G

S

Y

I

L

I

A

N

V

V

K

A

A

M

L

A

E

D

H

W

D

D

H

S

Y

Y

L

R

N

T

E

Q

V

V

S

H

A

Q

W

W

E

E

-

L

R

D

R

R

Y

Y

L

L

binding adenosine-5'-triphosphate: G117 (≠ A149), E170 (≠ D211), F185 (≠ L226), K186 (≠ L227), Y187 (≠ H228), N233 (≠ H274), S235 (= S276), S315 (≠ A355), R317 (= R357)
binding magnesium ion: E119 (= E151), H231 (= H272), E319 (= E359)

8ooxB Glutamine synthetase (see paper)
30% identity, 93% coverage: 28:457/461 of query aligns to 11:435/438 of 8ooxB

query
sites
8ooxB

K

E

A

E

A

K

G

N

V

V

R

R

D

F

V

V

E

R

C

L

L

Q

F

F

A

V

D

D

V

V

T

Q

G

G

Y

I

P

P

R

K

G

N

K

V

L

A

M

I

P

P

A

V

A

-

S

-

F

-

A

-

G

-

G

G

E

Q

L

L

R

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I

-

C

-

Q

K

A

A

I

L

P

G

M

P

Q

G

C

I

V

H

T

F

G

D

E

G

Y

S

S

S

Y

I

D

E

P

G

I

F

F

V

P

R

D

I

S

E

D

E

P

S

D

D

V

M

R

V

L

L

V

R

P

P

D

D

L

P

A

D

T

T

L

F

K

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L

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W

W

A

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-

G

S

N

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A

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Y

A

L

R

A

L

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I

H

C

D

D

C

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F

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L

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D

G

G

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K

L

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M

F

G

A

C

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P

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Q

V

V

L

L

K

K

Q

K

V

N

V

M

A

E

R

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Y

A

R

A

A

K

R

L

G

G

L

Y

T

V

P

M

V

N

I

T

A

G

P

P

E

E

I

M

E
|
E

F

F

Y

F

L

L

T

F

A

K

A

R

M

Q

T

D

D

-

P

-

A

G

Q

M

P

P

L

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A

N

Y

I

P

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V

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D

R

R

G

G

R

Y

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F

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D

V

L

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A

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I

A

D

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L

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A

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-

F

-

W

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-

A

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L

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G

E

L

M

G

G

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A

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D

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T

A

W

A

L

H

H

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|
E

V

V

G

A

Q

F

S

G

Q

Q

Y

H

E
|
E

I

I

N

D

L

F

L

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Y

G

D

D

N

P

A

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L

A

A

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F

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M

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M

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N

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Y

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W

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N

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L

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V

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P

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I

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N

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K

N

N

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Y

Y

D

K

L

L

A

T

R

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G

E

-

E

-

R

-

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-

K

-

L

-

G

-

I

-

G

-

M

L

L

P

P

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G

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T

F

L

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K

T

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A

A

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D

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C

M

F

A

K

H

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S

D

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H

L

A

L

P

V

R

S

L

A

L

L

G

G

E

E

R

H

F

V

V

S

R

Q

G

S

Y

I

L

I

A

N

V

V

K

A

A

M

L

A

E

D

H

W

D

D

H

S

Y

Y

L

R

N

T

E

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V

V

S

H

A

Q

W

W

E

E

-

L

R

D

R

R

Y

Y

L

L

binding magnesium ion: E129 (= E153), E183 (= E216), E190 (= E223)

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
34% identity, 79% coverage: 92:453/461 of query aligns to 56:390/396 of 5dm3C

query
sites
5dm3C

L

M

V

K

P

P

D

D

L

L

A

A

T

T

L

L

K

R

P

C

T

V

P

P

W

W

A

L

S

E

V

G

P

T

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-

Y

-

L

-

A

A

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M

H

V

D

L

C

C

F

D

E

L

L

L

D

D

G

H

S

R

L

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-

H

-

A

-

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C

V

E

P

F

H

A

A

P

P

R

R

S

A

V

I

L

L

K

K

Q

R

V

Q

V

L

A

A

R

R

Y

L

R

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A

A

R

M

G

G

L

L

T

E

P

A

V

I

I

M

A

A

P

T

E
|
E

I

L

E
|
E

F

F

Y

F

L

L

T

F

A

E

A

K

M

S

T

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D

D

P

E

A

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L

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Y

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G

-

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R

G

K

G

G

R

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F

E

R

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G

T

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K

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A

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L

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L

A

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P

F

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W

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D

N

A

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L

L

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H

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-

D

-

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-

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G

I

I

R

P

A

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D

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T

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W

T

L

K

H

G

E
|
E

V

A

G

G

Q

A

S

G

Q

Q

Y

E

E
|
E

I

L

N

N

L

I

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x
R

H
x
C

G

A

D

K

P

A

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L

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Y

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K

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W

I

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A

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H

A

A

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|
H

L

I

H
|
H

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|
S

V

L

V

W

D

K

A

-

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Q

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G

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A

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M

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L

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-

K

H

H

Y

Y

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L

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A

G

G

L

L

Q

L

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K

Y

Y

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A

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D

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A

T

L

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F

Y

L

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A

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P

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L

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N

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S

Y

Y

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K

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W

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D

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|
R

T

T

T

A

G

G

L

F

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R

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L

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C

D

A

S

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G

P

T

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A

A

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R
|
R

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I

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|
E

N

C

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|
R

L

I

A

G

G

G

A

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D

D

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L

N

N

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Y

L

L

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A

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M

A

A

A

G

T

Q

L

L

A

A

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G

L

I

A

K

G

G

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D

E

E

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C

L

L

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A

P

L

S

P

E

P

P

P

V

A

Q

S

G

G

N

L

G

-

Y

-

D

-

L

-

A

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-

G

-

L

I

P

P

R

Q

T

N

F

L

S

R

T

D

A

A

H

M

D

E

Q

A

M

L

A

R

H

G

S

S

E

T

H

M

A

L

P

R

R

E

L

A

L

M

G

G

E

E

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D

F

V

V

V

R

D

G

H

Y

Y

L

V

A

R

V

A

K

A

A

E

L

V

E

E

H

L

D

E

H

D

Y

F

L

Q

N

R

E

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V

V

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S

A

D

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Y

E

E

active site: E115 (= E151), E117 (= E153), E162 (= E216), E169 (= E223), H218 (= H272), R286 (= R342), E303 (= E359), R305 (= R361)
binding adenosine-5'-diphosphate: R173 (≠ L227), C174 (≠ H228), H220 (= H274), S222 (= S276), R301 (= R357)

5dm3A Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
32% identity, 79% coverage: 92:453/461 of query aligns to 52:368/374 of 5dm3A

query
sites
5dm3A

L

M

V

K

P

P

D

D

L

L

A

A

T

T

L

L

K

R

P

C

T

V

P

P

W

W

A

-

S

-

V

-

P

-

R

-

Y

-

L

-

A

-

V

-

H

-

D

-

C

-

F

-

E

-

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L

D

E

G

G

S

T

-

A

-

M

-

V

L

L

C

C

E

D

F

L

-

L

-

H

-

A

-

E

-

V

-

P

-

H

A

A

P

P

R

R

S

A

V

I

L

L

K

K

Q

R

V

Q

V

L

A

A

R

R

Y

L

R

E

A

A

R

M

G

G

L

L

T

E

P

A

V
x
I

I

M

A

A

P

T

E
|
E

I

L

E
|
E

F

F

Y

F

L

L

T

F

A

E

A

K

M

S

T

L

D

D

P

T

A

T

Q

K

P

E

L

E

A

-

Y

-

P

-

V

-

G

-

R

-

G

-

R

-

P

-

E

-

V

-

G

-

Q

-

S

-

A

-

F

-

S

-

M

-

N

-

L

-

N

H

E

V

L

L

A

R

P

P

F

L

W

R

D

N

A

H

L

L

H

H

A

A

I

-

D

-

G

G

L

I

G

P

I

V

R
x
E

A

G

D

T

T

K

W

-

L

-

H

-

E

-

V

-

G

G

Q
x
E

S

G

Q

Q

Y

E

E
|
E

I

L

N

N

L

I

L
x
R

H
x
C

G

A

D

K

P

A

V

L

A

D

V

T

A

A

D

D

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Y

A

H

F

T

L

I

F

A

K

K

T

H

A

A

A

T

R

K

E

E

I

I

A

A

L

W

Q

Q

H

Q

G

G

L

R

N

A

A

V

V

T

F

F

M

L

A

S

K

K

P

W

I

H

S

H

G

A

Q

H

A

A

G

G

S

S

M

S

H
|
H

L

I

H
|
H

Q

Q

S
|
S

V

L

V

W

D

K

A

-

Q

Q

G

G

R

L

N

P

I

A

F

F

-

H

S

D

Q

E

A

R

D

D

G

A

S

L

A

G

S

M

E

S

A

A

F

L

L

M

-

K

H

H

Y

Y

L

L

G

A

G

G

L

L

Q

L

A

K

Y

Y

T

A

P

P

D

D

L

Y

A

T

L

Y

V

F

Y

L

A

A

P

P

S

Y

V

L

N

N

S

S

Y

Y

R

K

R

R

Y

F

V

-

A

-

G

-

S

-

Q

-

A

Q

P

P

I

T

N

R

V

T

A

V

W

W

G

S

H

V

D

D

N

N

R
|
R

T

T

T

A

G

G

L

F

R

R

V

L

P

C

D

A

S

E

P

G

P

T

A

R

A

A

R

V

R
|
R

V

I

E
|
E

N

C

R
|
R

L

I

A

G

G

G

A

S

D

D

A

L

N

N

P

P

Y

Y

L

L

A

A

M

M

A

A

A

G

T

Q

L

L

A

A

G

G

L

I

A

K

G

G

I

I

D

E

E

E

R

C

L

L

P

A

P

L

S

P

E

P

P

P

V

-

Q

-

G

-

N

-

G

-

Y

-

D

-

L

-

A

A

R

G

G

L

L

I

P

P

R

Q

T

N

F

L

S

R

T

D

A

A

H

M

D

E

Q

A

M

L

A

R

H

G

S

S

E

T

H

M

A

L

P

R

R

E

L

A

L

M

G

G

E

E

R

D

F

V

V

V

R

D

G

H

Y

Y

L

V

A

R

V

A

K

A

A

E

L

V

E

E

H

L

D

E

H

D

Y

F

L

Q

N

R

E

V

V

V

S

S

A

D

W

Y

E

E

active site: E107 (= E151), E109 (= E153), E146 (≠ Q219), E150 (= E223), H199 (= H272), R265 (= R342), E282 (= E359), R284 (= R361)
binding adenosine-5'-diphosphate: I103 (≠ V147), E141 (≠ R209), R154 (≠ L227), C155 (≠ H228), H201 (= H274), S203 (= S276), R280 (= R357)

Query Sequence

>WP_218017304.1 NCBI__GCF_001592305.1:WP_218017304.1
MTLPASPSWSHLNLSQDADRAQLAAHFKAAGVRDVECLFADVTGYPRGKLMPAASFAAGG
ELRICQAIPMQCVTGEYSYDPIFPDSDPDVRLVPDLATLKPTPWASVPRYLAVHDCFELD
GSLCEFAPRSVLKQVVARYRARGLTPVIAPEIEFYLTAAMTDPAQPLAYPVGRGGRPEVG
QSAFSMNLLNELAPFWDALHAAIDGLGIRADTWLHEVGQSQYEINLLHGDPVAVADQAFL
FKTAAREIALQHGLNAVFMAKPISGQAGSSMHLHQSVVDAQGRNIFSQADGSASEAFLHY
LGGLQAYTPDLALVYAPSVNSYRRYVAGSQAPINVAWGHDNRTTGLRVPDSPPAARRVEN
RLAGADANPYLAMAATLAAGLAGIDERLPPSEPVQGNGYDLARGLPRTFSTAHDQMAHSE
HAPRLLGERFVRGYLAVKALEHDHYLNEVSAWERRYLLPQA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory