SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_226992378.1 NCBI__GCF_000429905.1:WP_226992378.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
54% identity, 71% coverage: 17:114/139 of query aligns to 8:104/200 of 6j2lA

query
sites
6j2lA

E

E

L

L

D

D

F

W

E

E

K

K

T

T

G

D

G

G

L

L

L

M

P

P

A

V

I

I

C

V

Q

Q

D

H

A

A

E

V

T

S

G

G

E

E

V

V

L

L

M

M

L

L

A

G

F

Y

M

M

N

N

K

P

E

E

S

A

W

L

E

D

K

K

T

T

L

I

E

E

T

S

G

G

M

K

A

V

T

T

Y

F

W

F

S

S

R

R

S

T

R

K

Q

Q

E

R

L

L

W

W

T

I

K

K

G

G

L

E

T

T

S

S

G

G

N

N

V

F

Q

L

K

N

V

V

K

V

E

S

I

I

R

A

V

P

D
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D

C

C

D
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D

D

N

D
|
D

T

T

I

L

L

L

L

V

K

L

V

A

E

N

Q

P

I

I

G

-

G

G

A

P

A

T

C
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C

H

H

T

K

G

G

H

T

R

S

S

S

C
|
C

F

F

binding zinc ion: D79 (= D88), D81 (= D90), D83 (= D92), C96 (= C106), C103 (= C113)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

6j2lB Crystal structure of bi-functional enzyme (see paper)
51% identity, 71% coverage: 17:114/139 of query aligns to 7:106/185 of 6j2lB

query
sites
6j2lB

E

E

L

L

D

D

F

W

E

E

K

K

T

T

G

D

G

G

L

L

L

M

P

P

A

V

I

I

C

V

Q

Q

D

H

A

A

E

V

T

S

G

G

E

E

V

V

L

L

M

M

L

L

A

G

F

Y

M

M

N

N

K

P

E

E

S

A

W

L

E

D

K

K

T

T

L

I

E

E

T

S

G

G

M

K

A

V

T

T

Y

F

W

F

S

S

R

R

S

T

R

K

Q

Q

E

R

L

L

W

W

T

I

K

K

G

G

L

E

T

T

S

S

G

G

N

N

V

F

Q

L

K

N

V

V

K

V

E

S

I

I

R

A

V

P

D
|
D

C

C

D
|
D

D

N

D
|
D

T

T

I

L

L

L

L

V

K

L

V

A

E

N

Q

P

I

I

G

G

G

P

A

S

A

S

C

C

-

F

-

G

H

N

T

T

G

A

H

H

R

Q

S

W

C

L

F

F

binding zinc ion: D78 (= D88), D80 (= D90), D82 (= D92)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
40% identity, 78% coverage: 25:132/139 of query aligns to 17:111/213 of 7bgmA

query
sites
7bgmA

G

G

L

L

L

A

P

V

A

A

I

I

C

A

Q

Q

D

N

A

V

E

D

T

T

G

G

E

A

V

I

L

L

M

M

L

Q

A

G

F

F

M

A

N

N

K

R

E

E

S

A

W

V

E

A

K

T

T

T

L

I

E

S

T

S

G

R

M

K

A

A

T

T

Y

F

W

Y

S

S

R

R

S

S

R

R

Q

S

E

S

L

L

W

W

T

T

K

K

G

G

L

E

T

T

S

S

G

N

N

N

V

F

Q

I

K

N

V

V

K

H

E

D

I

V

R

F

V

L

D
|
D

C

C

D
|
D

D

R

D
|
D

T

S

I

I

L

I

L

Y

K

-

V

L

E

G

Q

K

I

P

G

D

G

G

A

P

A

T

C
|
C

H

H

T

T

G

G

H

A

R

E

S

T

C
|
C

F

Y

H

Y

K

T

L

-

V

-

E

-

G

-

D

-

K

-

L

-

T

-

V

-

V

-

G

-

E

-

P

P

V

V

F

F

D

D

binding zinc ion: D80 (= D88), D82 (= D90), D84 (= D92), C97 (= C106), C104 (= C113)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
40% identity, 78% coverage: 25:132/139 of query aligns to 15:109/204 of 7bgnA

query
sites
7bgnA

G

G

L

L

L

A

P

V

A

A

I

I

C

A

Q

Q

D

N

A

V

E

D

T

T

G

G

E

A

V

I

L

L

M

M

L

Q

A

G

F

F

M

A

N

N

K

R

E

E

S

A

W

V

E

A

K

T

T

T

L

I

E

S

T

S

G

R

M

K

A

A

T

T

Y

F

W

Y

S

S

R

R

S

S

R

R

Q

S

E

S

L

L

W
|
W

T

T

K
|
K

G
|
G

L

E

T
|
T

S
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S

G

N

N

N

V

F

Q

I

K

N

V

V

K

H

E

D

I

V

R

F

V

L

D
|
D

C

C

D
|
D

D

R

D
|
D

T

S

I

I

L

I

L

Y

K

-

V

L

E

G

Q

K

I

P

G

D

G

G

A

P

A
x
T

C
|
C

H
|
H

T

T

G

G

H

A

R

E

S

T

C
|
C

F

Y

H

Y

K

T

L

-

V

-

E

-

G

-

D

-

K

-

L

-

T

-

V

-

V

-

G

-

E

-

P

P

V

V

F

F

D

D

binding adenosine monophosphate: W60 (= W70), K62 (= K72), G63 (= G73), T65 (= T75), S66 (= S76), T94 (≠ A105), H96 (= H107)
binding zinc ion: D78 (= D88), D80 (= D90), D82 (= D92), C95 (= C106), C102 (= C113)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

Query Sequence

>WP_226992378.1 NCBI__GCF_000429905.1:WP_226992378.1
MRGFETSSRQQEEIMIELDFEKTGGLLPAICQDAETGEVLMLAFMNKESWEKTLETGMAT
YWSRSRQELWTKGLTSGNVQKVKEIRVDCDDDTILLKVEQIGGAACHTGHRSCFHKLVEG
DKLTVVGEPVFDPKEVYKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory