SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_231861429.1 NCBI__GCF_000058485.1:WP_231861429.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
38% identity, 89% coverage: 24:258/263 of query aligns to 11:248/249 of P12282

query
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P12282

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R14 (= R27) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ G57) mutation to A: No effect.
R73 (= R86) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ A141) mutation to A: No effect.; mutation to Y: No activity.
D130 (≠ H143) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C155) mutation to A: No effect.
C172 (= C183) mutation to A: No zinc bound and no enzyme activity.
C175 (= C186) mutation to A: No zinc bound and no enzyme activity.
C187 (≠ W195) mutation to A: No effect.
C231 (≠ V241) mutation to A: No effect.
C244 (= C254) mutation to A: No zinc bound and almost no enzyme activity.
C247 (= C257) mutation to A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
37% identity, 89% coverage: 24:258/263 of query aligns to 10:240/240 of 1jwbB

query
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1jwbB

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active site: R13 (= R27), D129 (≠ H143)
binding adenosine monophosphate: G37 (= G51), G39 (= G53), G40 (= G54), D61 (≠ H75), F62 (≠ P76), K85 (≠ R99), L108 (≠ D122), C127 (≠ A141), T128 (≠ R142), D129 (≠ H143), N130 (= N144), V133 (≠ E147)
binding zinc ion: C171 (= C183), C236 (= C254), C239 (= C257)

1jw9B Structure of the native moeb-moad protein complex (see paper)
37% identity, 89% coverage: 24:258/263 of query aligns to 10:240/240 of 1jw9B

query
sites
1jw9B

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active site: R13 (= R27), D129 (≠ H143)
binding zinc ion: C171 (= C183), C236 (= C254), C239 (= C257)

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
42% identity, 88% coverage: 6:237/263 of query aligns to 40:279/460 of O95396

query
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O95396

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158:238 (vs. 120:199, 37% identical) Interaction with NFS1
C239 (vs. gap) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
35% identity, 94% coverage: 16:262/263 of query aligns to 60:323/482 of O59954

query
sites
O59954

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T

C

A

V

V

Q

L

A

L

A

G

V

K

P

P

L

L

V

V

V

S

A

A

A

S

M

A

N

L

A

R

T

T

E
|
E

A

G

Y

Q

L

L

M

M

V

V

V

L

-

N

-

P

-

Q

-

P

-

G

-

D

R

K

P

T

G

G

E

G

P

P

C

C

L

Y

R

R

C

C

V

V

F

F

G

P

E

K

G

P

D

P

P

P

A

A

W

N

E

S

P

V

L

T

G

S

F

C

P

A

-

D

-

G

V

I

L

L

G

G

A

P

V

V

A

V

G
|
G

T

T

V

M

G

G

C

V

L

L

A

Q

A

A

T

S

E

E

A

A

L

I

K

K

I

V

A

L

G

T

G

S

F

A

G

G

E

E

S

S

A

V

V

E

G

A

R

T

-

P

-

S

-

L

L

L

V

I

H

F

L

S

D

A

L

Y

W

S

D

S

V

P

D

Q

L

F

R

R

T

T

P

I

R

K

T

L

R

R

-

S

R

R

D

R

P

P

A

N

C

C

P

A

D

V

C

C

G

S

P

A

G

E

S

A

T

T

G82 (≠ A38) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G56) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R86) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ A141) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (= E171) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G208) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
34% identity, 93% coverage: 15:258/263 of query aligns to 62:310/453 of Q9VLJ8

query
sites
Q9VLJ8

T
|
T

P

K

P

L

T

T

A

N

E

D

T

D

S

I

R

A

R

R

F

Y

D

S

R

R

Q

Q

V

L

T

I

I

L

P

P

G

D

F

F

G

G

V

V

A

Q

A

G

Q

Q

R

L

R

K

L

L

S

K

Q

N

A

S

T

S

V

V

L

L

V

I

A

V

G

G

V

L

G

G

V

L

G

G

G

C

A

P

A

A

T

Q

Y

Y

L

L

A

A

G

G

V

C

G

G

R

H

L

L

I

G

L

L

I

V

H

D

P

Y

G

D

D

E

L

V

A

E

E

R

P

S

D

N

L

F

N

H

R

R

Q

Q

T

I

L

L

M

H

R

S

P

E

E

D

R

R

V

C

G

G

E

M

P

S

R

K

V

A

F

E

C

S

A

A

A

R

D

I

T

A

L

L

R

L

A

E

H

L

H

N

P

P

G

H

V

C

E

E

I

I

V

Q

A

C

V

H

D

S

R

R

D

M

L

L

T

Y

D

P

P

H

A

N

V

A

P

M

G

H

L

I

I

I

A

R

E

G

A

Y

D

D

V

V

V

L

D

D

A

C

R

T

H

D

N

N

S

V

P

P

E

T

R

R

Y

Y

L

L

I

L

N

S

R

D

L

A

C

C

V

V

Q

M

A

L

A

S

V

K

P

P

L

L

V

V

V

S

A

G

A

S

M

A

N

L

A

K

T

M

E

D

A

G

Y

Q

L

L

M

T

V

V

V

Y

R

N

P

Y

G

A

E

N

-

G

P

P

C

C

L

Y

R

R

C

C

V

I

F

F

-

P

-

V

-

P

-

E

-

A

-

V

-

T

-

N

-

C

G

G

E

D

G

G

D

G

P

-

A

-

W

-

E

-

P

-

L

-

G

-

F

-

P

-

V

V

L

L

G

G

A

A

V

V

A

T

G

G

T

T

V

I

G

G

C

A

L

M

A

Q

A

A

T

L

E

E

A

A

L

I

K

K

I

V

A

I

G

V

G

G

F

M

G

G

E

D

S

V

A

L

V

A

G

G

R

R

L

L

V

L

H

I

L

F

D

D

L

G

W

G

D

S

V

G

D

V

L

F

R

R

T

N

P

I

R

R

T

I

R

R

-

S

R

K

D

R

P

P

A

N

C

C

P

H

D

M

C

C

G

S

T62 (= T15) modified: Phosphothreonine

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
38% identity, 90% coverage: 24:260/263 of query aligns to 12:251/270 of D4GSF3

query
sites
D4GSF3

R

R

F

Y

D

S

R

R

Q

H

V

I

T

I

I

M

P

D

G

E

F

V

G

G

V

P

A

E

A

G

Q

Q

R

G

R

R

L

L

S

L

Q

S

A

S

T

R

V

V

L

V

V

V

A

V

G

G

V

A

G

G

V

L

G

G

G

A

A

P

A

A

A

I

T

Q

Y

Y

L

L

A

A

A

V

G

G

V

V

G

G

R

E

L

L

I

V

L

V

I

V

H

D

P

D

G

D

D

V

L

V

A

E

E

R

P

S

D

N

L

L

N

Q

R

R

Q

Q

T

V

L

V

M

H

R

C

P

D

E

D

R

D

V

V

G

G

E

T

P

P

R

K

V

A

F

E

C

S

A

A

D

A

T

F

L

V

R

R

A

G

H

L

H

N

P

P

G

D

V

V

E

S

I

V

V

E

A

P

V

V

D

E

R

A

D

R

L

V

T

D

D

K

P

S

A

N

V

V

P

H

G

E

L

V

I

V

A

A

E

G

A

S

D

D

V

V

D

D

A

A

R

S

H

D

N

N

S

F

P

P

E

T

R

R

Y

Y

L

L

I

L

N

N

R

D

L

V

C

C

V

R

Q

F

A

E

A

G

V

I

P

P

L

L

V

V

V

H

A

G

A

A

M

I

N

Y

A

K

T

F

E

E

A

G

Y

Q

L

A

M

T

V

T

V

L

R

V

P

P

G

D

E

G

P

P

C

C

L

Y

R

R

C

C

V

L

F

F

G

P

E

E

G

-

D

-

P

-

A

A

W

P

E

E

P

P

L

G

G

T

F

V

P

P

-

D

-
x
C

-

A

-

T

-

G

V

V

L

L

G

G

A

V

V

L

A

P

G

G

T

T

V

V

G

G

C

C

L

I

A

Q

A

A

T

T

E

E

A

A

L

M

K

K

I

L

A

L

G

L

G

D

F

E

G

G

E

E

S

A

A

L

V

D

G

G

R

R

L

L

V

L

H

F

L

Y

D

D

L

A

W

M

D

D

V

M

D

T

L

F

R

E

T

T

P

V

R

P

T

Y

R

R

R

T

D

N

P

P

A

D

C

C

P

P

D

V

C

C

G

G

P

E

G

G

C188 (vs. gap) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
36% identity, 92% coverage: 18:258/263 of query aligns to 4:253/271 of Q72J02

query
sites
Q72J02

T

T

A

K

E

E

T

E

S

L

R

D

R

R

F

Y

D

H

R

R

Q

Q

V

M

T

I

I

L

P

P

G

Q

F

V

G

G

V

P

A

E

A

G

Q

Q

R

E

R

R

L

L

S

K

Q

R

A

A

T

S

V

V

L

V

V

V

A

V

G

G

V

A

G

G

V

L

G

G

G

V

A

P

A

V

A

L

T

Q

Y

Y

L

L

A

V

A

A

G

G

V

V

G

G

R

R

L

V

I

G

L

V

I

V

H

E

P

M

G

D

D

R

L

V

A

E

E

V

P

S

D

N

L

L

N

H

R

R

Q

Q

T

V

L

L

M

Y

R

T

P

T

E

E

R

D

V

V

G

G

E

E

P

P

R

K

V

A

F

L

C

V

A

A

A

Q

D

K

T

R

L

L

R

Q

A

A

H

L

H

N

P

P

G

L

V

V

E

R

I

V

V

E

A

A

V

Y

D

P

R

V

D

R

L

L

T

T

D

S

P

E

A

N

V

A

P

L

G

E

L

I

I

L

A

R

E

P

A

Y

D

D

V

L

V

V

D

D

A

A

R

S

H

D

N

N

S

F

P

P

E

T

R

R

Y

Y

L

L

I

V

N

N

R

D

L

A

C

A

V

V

Q

L

A

L

A

G

V

K

P

P

L

L

V

V

V

F

A

G

A

A

M

I

N

Y

A

Q

T

F

E

D

A

G

Y

Q

L

V

M

A

V

V

V

F

R

H

-

H

-

P

-

T

-

L

P

H

G

G

E

E

-

M

-

G

P

P

C

C

L

Y

R

R

C

C

V

L

F

F

G

P

E

K

G

P

D

P

-

P

-

G

-

A

-

V

P

P

A

S

W
x
C

E

A

P

E

L

A

G

G

F

-

P

-

V

V

L

F

G

G

A

V

V

L

A

P

G

A

T

V

V

V

G

G

C

S

L

L

A

M

A

A

T

A

E

E

A

A

L

L

K

K

I

V

A

L

G

L

G

G

F

I

G

G

E

K

S

P

A

L

V

A

G

G

R

H

L

L

V

L

H

L

L

Y

D

D

L

A

W

L

D

E

V

A

D

S

L

F

R

R

T

K

P

L

R

T

T

V

R

R

D

N

P

P

A

R

C

C

P

P

D

V

C

C

G

G

C192 (≠ W195) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
34% identity, 86% coverage: 24:250/263 of query aligns to 10:217/217 of 1jwaB

query
sites
1jwaB

R

R

F

Y

D

N

R
|
R

Q

Q

V

I

T

I

I

L

P

R

G

G

F

F

G

D

V

F

A

D

A

G

Q

Q

R

E

R

A

L

L

S

K

Q

D

A

S

T

R

V

V

L

L

V

I

A

V

G

G

V

L

G
|
G

V

L

G

G

G

C

A

A

A

S

T

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

R

N

L

L

I

T

L

L

I

L

H
x
D

P
x
F

G

D

D

T

L

V

A

S

E

L

P

S

D

N

L

L

N

Q

R
|
R

Q

Q

T

T

L

L

M

H

R

S

P

D

E

A

R

T

V

V

G

G

E

Q

P

P

R
x
K

V

V

F

E

C

S

A

A

A

R

D

D

T

A

L

L

R

T

A

R

H

I

H

N

P

P

G

H

V

I

E

A

I

I

V

T

A

P

V

V

D

N

R

A

D
x
L

L

L

T

D

D

D

P

A

A

E

V

L

P

A

G

A

L

L

I

I

A

A

E

E

A

H

D

D

V

L

V

V

V

L

D

D

A

C

R

T

H
x
D

N
|
N

S

V

P

A

E
x
V

R

R

Y

N

L

Q

I

L

N

N

R

A

L

G

C

C

V

F

Q

A

A

A

A

K

V

V

P

P

L

L

V

V

V

S

A

G

A

A

M

A

N

I

A

R

T

M

E

E

A

G

Y

Q

L

I

M

T

V

V

V

F

R

-

P

-

G

-

E

-

P

-

C

-

L

-

R

-

C

-

V

-

F

-

G

-

E

-

G

-

D

-

P

-

A

T

W

Y

E

E

P

A

L

-

G

-

F

-

P

G

V

V

L

M

G

A

A

P

V

L

A

I

G

G

T

V

V

I

G

G

C

S

L

L

A

Q

A

A

T

M

E

E

A

A

L

I

K

K

I

M

A

L

G

A

G

G

F

Y

G

G

E

K

S

P

A

A

V

S

G

G

R

K

L

I

V

V

H

M

L

Y

D

D

L

A

W

M

D

T

V

C

D

Q

L

F

R

R

T

E

P

M

R

K

T

L

R

M

R

R

active site: R13 (= R27), D129 (≠ H143)
binding adenosine-5'-triphosphate: G39 (= G53), G40 (= G54), D61 (≠ H75), F62 (≠ P76), R72 (= R86), K85 (≠ R99), L108 (≠ D122), D129 (≠ H143), N130 (= N144), V133 (≠ E147)

1zud3 Structure of this-thif protein complex (see paper)
34% identity, 89% coverage: 24:258/263 of query aligns to 8:239/240 of 1zud3

query
sites
1zud3

R

R

F

Y

D

S

R
|
R

Q

Q

V

I

T
x
L

I

L

P
x
D

G

D

F

I

G

A

V

L

A

D

A

G

Q

Q

R

Q

R

K

L

L

S

L

Q

D

A

S

T

Q

V

V

L

L

V

I

A

I

G

G

V

L

G

G

V

L

G

G

G

T

A

P

A

A

T

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

R

T

L

L

I

V

L

L

I

A

H

D

P

D

G

D

D

D

L

V

A

H

E

L

P

S

D

N

L

L

N

Q

R

R

Q

Q

T

I

L

L

M

F

R

T

P

T

E

E

R

D

V

I

G

D

E

R

P

P

R

K

V

S

F

Q

C

V

A

S

A

Q

D

Q

T

R

L

L

R

T

A

Q

H

L

H

N

P

P

G

D

V

I

E

Q

I

L

V

T

A

A

V

L

D

Q

R

Q

D

R

L

L

T

T

D

G

P

E

A

A

V

L

P

K

G

D

L

A

I

V

A

A

E

R

A

A

D

D

V

V

V

L

D

D

A

C

R

T

H
x
D

N

N

S

M

P

A

E

T

R

R

Y

Q

L

E

I

I

N

N

R

A

L

A

C

C

V

V

Q

A

A

L

A

N

V

T

P

P

L

L

V

I

V

T

A

A

A

S

M

A

N

V

A

G

T

F

E

G

A

G

Y

Q

L

L

M

M

V

V

V

L

R

T

P

P

-

P

-

W

G

E

E

Q

P

G

C
|
C

L

Y

R

R

C

C

V

L

F

W

G

P

E

D

G

N

D

Q

P

-

A

-

W

-

E

E

P

P

L

T

G

A

F

G

P

V

V

V

L

-

G

G

A

P

V

V

A

V

G

G

T

V

V

M

G

G

C

T

L

L

A

Q

A

A

T

L

E

E

A

A

L

I

K

K

I

L

A

L

G

S

G

G

F

I

G

-

E

E

S

T

A

P

V

A

G

G

R

E

L

L

V

R

H

L

L

F

D

D

L

G

W

K

D

S

V

S

D

Q

L

W

R

R

T

S

P

L

R

A

T

L

R

R

D

A

P

S

A

G

C
|
C

P

P

D

V

C
|
C

G

G

active site: R11 (= R27), D127 (≠ H143)
binding calcium ion: L14 (≠ T30), D16 (≠ P32)
binding zinc ion: C169 (= C183), C235 (= C254), C238 (= C257)

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 89% coverage: 24:258/263 of query aligns to 8:244/251 of P30138

query
sites
P30138

R

R

F

Y

D

S

R

R

Q

Q

V

I

T

L

I

L

P

D

G

D

F

I

G

A

V

L

A

D

A

G

Q

Q

R

Q

R

K

L

L

S

L

Q

D

A

S

T

Q

V

V

L

L

V

I

A

I

G

G

V

L

G

G

V

L

G

G

G

T

A

P

A

A

T

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

R

T

L

L

I

V

L

L

I

A

H

D

P

D

G

D

D

D

L

V

A

H

E

L

P

S

D

N

L

L

N

Q

R

R

Q

Q

T

I

L

L

M

F

R

T

P

T

E

E

R

D

V

I

G

D

E

R

P

P

R

K

V

S

F

Q

C

V

A

S

A

Q

D

Q

T

R

L

L

R

T

A

Q

H

L

H

N

P

P

G

D

V

I

E

Q

I

L

V

T

A

A

V

L

D

Q

R

Q

D

R

L

L

T

T

D

G

P

E

A

A

V

L

P

K

G

D

L

A

I

V

A

A

E

R

A

A

D

D

V

V

V

L

D

D

A

C

R

T

H

D

N

N

S

M

P

A

E

T

R

R

Y

Q

L

E

I

I

N

N

R

A

L

A

C

C

V

V

Q

A

A

L

A

N

V

T

P

P

L

L

V

I

V

T

A

A

A

S

M

A

N

V

A

G

T

F

E

G

A

G

Y

Q

L

L

M

M

V

V

V

L

R

T

P

P

-

P

-

W

G

E

E

Q

P

G

C
|
C

L

Y

R

R

C
|
C

V

L

F
x
W

G

P

E

D

G

N

-

Q

D

E

P

P

A

E

W

R

E

N

P
x
C

L

R

G

T

F

A

P

G

V

V

L

V

G

G

A

P

V

V

A

V

G

G

T

V

V

M

G

G

C

T

L

L

A

Q

A

A

T

L

E

E

A

A

L

I

K

K

I

L

A

L

G

S

G

G

F

I

G

-

E

E

S

T

A

P

V

A

G

G

R

E

L

L

V

R

H

L

L

F

D

D

L

G

W

K

D

S

V

S

D

Q

L

W

R

R

T

S

P

L

R

A

T

L

R

R

D

A

P

S

A

G

C
|
C

P

P

D

V

C
|
C

G

G

C169 (= C183) binding Zn(2+)
C172 (= C186) binding Zn(2+)
W174 (≠ F188) mutation to A: No adenylation of ThiS.
C184 (≠ P197) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (= C254) binding Zn(2+)
C243 (= C257) binding Zn(2+)

1zfnA Structural analysis of escherichia coli thif (see paper)
34% identity, 89% coverage: 24:258/263 of query aligns to 8:244/244 of 1zfnA

query
sites
1zfnA

R

R

F

Y

D

S

R
|
R

Q

Q

V

I

T

L

I

L

P

D

G

D

F

I

G

A

V

L

A

D

A

G

Q

Q

R

Q

R

K

L

L

S

L

Q

D

A

S

T

Q

V

V

L

L

V

I

A
x
I

G
|
G

V

L

G
|
G

V

L

G

G

G

T

A

P

A

A

T

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

R

T

L

L

I

V

L

L

I

A

H
x
D

P

D

G

D

D

D

L

V

A

H

E

L

P

S

D

N

L

L

N

Q

R
|
R

Q
|
Q

T

I

L

L

M

F

R

T

P

T

E

E

R

D

V

I

G

D

E

R

P

P

R
x
K

V

S

F

Q

C

V

A

S

A

Q

D

Q

T

R

L

L

R

T

A

Q

H

L

H

N

P

P

G

D

V

I

E

Q

I

L

V

T

A

A

V

L

D

Q

R

Q

D

R

L

L

T

T

D

G

P

E

A

A

V

L

P

K

G

D

L

A

I

V

A

A

E

R

A

A

D

D

V

V

V

L

D

D

A

C

R
x
T

H
x
D

N

N

S

M

P

A

E
x
T

R

R

Y

Q

L

E

I

I

N

N

R

A

L

A

C

C

V

V

Q

A

A

L

A

N

V

T

P

P

L

L

V

I

V

T

A

A

A

S

M

A

N

V

A

G

T

F

E

G

A

G

Y

Q

L

L

M

M

V

V

V

L

R

T

P

P

-

P

-

W

G

E

E

Q

P

G

C
|
C

L

Y

R

R

C
|
C

V

L

F

W

G

P

E

D

G

N

-

Q

D

E

P

P

A

E

W

R

E

N

P

C

L

R

G

T

F

A

P

G

V

V

L

V

G

G

A

P

V

V

A

V

G

G

T

V

V

M

G

G

C

T

L

L

A

Q

A

A

T

L

E

E

A

A

L

I

K

K

I

L

A

L

G

S

G

G

F

I

G

-

E

E

S

T

A

P

V

A

G

G

R

E

L

L

V

R

H

L

L

F

D

D

L

G

W

K

D

S

V

S

D

Q

L

W

R

R

T

S

P

L

R

A

T

L

R

R

D

A

P

S

A

G

C
|
C

P

P

D

V

C
|
C

G

G

active site: R11 (= R27), D127 (≠ H143)
binding adenosine-5'-triphosphate: I34 (≠ A50), G35 (= G51), G37 (= G53), G38 (= G54), D59 (≠ H75), R70 (= R86), Q71 (= Q87), K83 (≠ R99), T126 (≠ R142), D127 (≠ H143), T131 (≠ E147)
binding zinc ion: C169 (= C183), C172 (= C186), C240 (= C254), C243 (= C257)

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
33% identity, 78% coverage: 18:221/263 of query aligns to 7:212/423 of 6yubA

query
sites
6yubA

T

S

A

K

E

E

T

E

S

I

R

E

R

R

F

Y

D

S

R

R

Q

Q

V

M

T

I

I

V

P

P

G

G

F

M

G

G

V

K

A

E

A

G

Q

Q

R

L

R

R

L

L

S

M

Q

N

A

A

T

K

V

V

L

L

V

I

A

I

G

G

V

A

G

G

V

L

G

G

G

C

A

P

A

A

T

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

R

T

L

I

I

G

L

I

I

V

H

D

P

G

G

D

D

S

L

V

A

E

E

T

P

S

D

N

L

L

N

H

R

R

Q

Q

T

V

L

A

M

H

R

A

P

T

E

K

R

R

V

V

G

G

E

M

P

L

R

K

V

V

F

D

C

S

A

L

A

I

D

T

T

H

L

L

R

I

A

E

H

I

H

N

P

P

G

L

V

P

E

V

I

Y

V

V

A

P

V

Y

D

R

R

F

D

D

L

L

T

T

D

P

P

Q

A

N

V

A

P

A

G

Q

L

I

I

I

A

K

E

P

A

W

D

D

V

V

V

I

V

L

D

D

A

C

R

T

H

D

N

N

S

P

P

A

E

T

R

R

Y

Y

L

L

I

I

N

S

R

D

L

V

C

C

V

V

Q

L

A

L

A

G

V

K

P

P

L

L

V

V

V

S

A

A

M

S

N

V

A

Q

T

K

E

S

A

G

Y

Q

L

L

M

I

V

V

V

L

-

N

-

C

-

P

-

T

-

P

-

Q

-

G

-

V

-

N

R

K

P

K

G

A

E

A

P

P

C
|
C

L

Y

R

R

C
|
C

V

C

F

F

G

K

E

K

G

-

D

-

P

P

A

G

W

-

E

-

P

-

L

-

G

-

F

-

P

-

V

I

L

M

G

G

A

P

V

V

A

V

G

G

T

M

V

M

G

G

C

V

L

A

A

Q

A

A

T

G

E

E

A

A

L

I

K

K

I

I

binding zinc ion: C183 (= C183), C186 (= C186)

Sites not aligning to the query:

binding zinc ion: 266, 269

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
33% identity, 78% coverage: 18:221/263 of query aligns to 8:211/289 of 6yubB

query
sites
6yubB

T

S

A

K

E

E

T

E

S

I

R

E

R

R

F

Y

D

S

R

R

Q

Q

V

M

T

I

I

V

P

P

G

G

F

M

G

G

V

K

A

E

A

G

Q

Q

R

L

R

R

L

L

S

M

Q

N

A

A

T

K

V

V

L

L

V

I

A

I

G

G

V

A

G

G

V

L

G

G

G

C

A

P

A

A

T

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

R

T

L

I

I

G

L

I

I

V

H

D

P

G

G

D

D

S

L

V

A

E

E

T

P

S

D

N

L

L

N

H

R

R

Q

Q

T

V

L

A

M

H

R

A

P

T

E

K

R

R

V

V

G

G

E

M

P

L

R

K

V

V

F

D

C

S

A

L

A

I

D

T

T

H

L

L

R

I

A

E

H

I

H

N

P

P

G

L

V

P

E

V

I

Y

V

V

A

P

V

Y

D

R

R

F

D

D

L

L

T

T

D

P

P

Q

A

N

V

A

P

A

G

Q

L

I

I

I

A

K

E

P

A

W

D

D

V

V

V

I

V

L

D

D

A

C

R

T

H

D

N

N

S

P

P

A

E

T

R

R

Y

Y

L

L

I

I

N

S

R

D

L

V

C

C

V

V

Q

L

A

L

A

G

V

K

P

P

L

L

V

V

V

S

A

A

M

S

N

V

A

Q

T

K

E

S

A

G

Y

Q

L

L

M

I

V

V

V

L

-

N

-

C

-

P

-

T

-

P

-

Q

-

G

-

V

-

N

R

K

P

K

G

A

E

A

P

P

C
|
C

L

Y

R

R

C
|
C

V

C

F

F

G

K

E

G

G

-

D

-

P

-

A

-

W

-

E

-

P

-

L

-

G

-

F

-

P

-

V

I

L

M

G

G

A

P

V

V

A

V

G

G

T

M

V

M

G

G

C

V

L

A

A

Q

A

A

T

G

E

E

A

A

L

I

K

K

I

I

binding zinc ion: C184 (= C183), C187 (= C186)

Sites not aligning to the query:

binding zinc ion: 265

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
27% identity, 94% coverage: 16:262/263 of query aligns to 38:294/440 of P38820

query
sites
P38820

P

P

P

L

T

S

A

L

E

E

T

E

S

Y

R

Q

R

R

F

Y

D

G

R

R

Q

Q

V

M

T

I

I

V

P

E

G

E

F

T

G

G

-

G

V

V

A

A

A

G

Q

Q

R

V

R

K

L

L

S

K

Q

N

A

T

T

K

V

V

L

L

V

V

A

V

G

G

V

A

G

G

V

L

G

G

G

C

A

P

A

A

A

L

T

P

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

R

Q

L

I

I

G

L

I

I

V

H

D

P

N

G

D

D

V

L

V

A

E

E

T

P

S

D

N

L

L

N

H

R

R

Q

Q

T

V

L

L

M

H

R

D

P

S

E

S

R

R

V

V

G

G

E

M

P

L

R

K

V

C

F

E

C

S

A

A

A

R

D

Q

T

Y

L

I

R

T

A

K

H

L

H

N

P

P

G

H

V

I

E

N

I

V

V

V

A

T

V

Y

D

P

R

V

D

R

L

L

T

N

D

S

P

S

A

N

V

A

P

F

G

D

L

I

I

F

A

K

E

G

A

Y

D

N

V

Y

V

I

V

L

D

D

A

C

R

T

H

D

N

S

S

P

P

L

E

T

R

R

Y

Y

L

L

I

V

N

S

R

D

L

V

C

A

V

V

Q

N

A

L

A

G

V

I

P

T

L

V

V

V

V

S

A

A

A

S

M

G

N

L

A

G

T

T

E

E

A

G

Y

Q

L

L

M

T

V

I

V

L

R

N

P

F

G

N

E

N

-

I

-

G

P

P

C

C

L

Y

R

R

C

C

V

F

F

Y

G

P

E

T

G

P

D

P

P

P

-

P

-

N

-

A

-

V

-

T

A

S

W
x
C

E

Q

P

E

L

G

G

G

F

-

P

-

V

V

L

I

G

G

A

P

V

C

A

I

G

G

T

L

V

V

G

G

C

T

L

M

A

M

A

A

T

V

E

E

A

T

L

L

K

K

I

L

A

I

G

L

G

G

F

I

G

Y

E

T

S

N

A

E

V

N

G

F

R

S

-

P

-

F

-

L

L

M

V

L

H

Y

L

S

D

G

L

F

W

P

D

Q

V

Q

D

S

L

L

R

R

T

T

P

F

R

K

T

M

R

R

-

G

R

R

D

Q

P

E

A

K

C

C

P

L

D

C

C

C

G

G

P

K

G

N

S

R

T

T

C225 (≠ W195) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca (see paper)
25% identity, 95% coverage: 9:258/263 of query aligns to 78:338/339 of 3h9jA

query
sites
3h9jA

I

I

L

I

P

P

V

G

Q

E

A

Y

T

N

P

N

P

S

T

T

A

E

E

N

T

N

-

R

-

Y

S

S

R

R

R

N

F

F

D

L

R

H

Q

Y

V

Q

T

S

I

Y

P

G

G

A

F

N

G

P

V

V

A

L

A

V

Q

Q

R

D

R

K

L

L

S

K

Q

N

A

A

T

K

V

V

L

V

V

I

A

L

G

G

V

C

G
|
G

V

I

G

G

G

N

A

H

A

V

A

S

T

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

R

E

L

I

I

I

L

L

I

I

H
x
D

P

N

G
x
D

D

Q

L

I

A

E

E

N

P

T

D

N

L

L

N

T

R
|
R

Q

Q

T

V

L

L

M

F

R

S

P

E

E

D

R

D

V

V

G

G

E

K

P

N

R
x
K

V

T

F

E

C

V

A

I

A

K

D

R

T

E

L

L

R

L

A

K

H

R

H

N

P

S

G

E

V

I

E

S

I

V

V

S

A

E

V

I

D

A

R

L

D
x
N

L
x
I

T

N

D

D

P

Y

A

T

V

D

P

L

G

H

L

K

I

V

A

P

E

E

A

A

D

D

V

I

-

W

V

V

D

S

A
|
A

R
x
D

H
|
H

N

P

S

F

P

N

E

L

R

I

Y

N

L

W

I

V

N

N

R

K

L

Y

C

C

V

V

Q

R

A

A

A

N

V

Q

P

P

L

Y

V

I

V

N

A

A

-

G

A

Y

M

V

N

N

A

D

T

I

E

A

A

V

Y

F

L

G

M

P

V

L

V

Y

R

V

P

P

G

G

E

K

P

T

-

G

C
|
C

L

Y

R

E

C

C

V

Q

F

K

G

K

E

E

G

N

-

I

-

D

-

H

-

K

-

I

-

K

-

L

-

I

D

N

P

S

A

R

W

F

E

K

P

P

L

A

G

T

F

F

P

A

V

P

L

V

G

N

A

N

V

V

A

A

G

A

T

A

V

-

G

-

C

-

L

L

A

C

A

A

T

A

E

D

A

V

L

I

K

K

I

F

A

I

G

G

G

K

F

Y

G

S

E

E

-

P

-

L

S

S

A

L

V

N

G

K

R
|
R

L

-

V

-

H

-

L

I

D
x
G

L

I

W

W

-

S

-

D

D

E

V

I

D

K

L

I

R

H

T

S

P

Q

R

N

T

M

R

G

R

R

D

S

P

P

A

V

C
|
C

P

S

D

V

C
|
C

G

G

active site: R157 (= R86)
binding diphosphomethylphosphonic acid adenosyl ester: G124 (= G53), G125 (= G54), D146 (≠ H75), D148 (≠ G77), R157 (= R86), K170 (≠ R99), N193 (≠ D122), I194 (≠ L123), A213 (= A141), D214 (≠ R142), H215 (= H143)
binding zinc ion: C257 (= C183), C334 (= C254), C337 (= C257)
binding : R313 (= R232), G315 (≠ D237)

3h5nA Crystal structure of e. Coli mccb + atp (see paper)
25% identity, 95% coverage: 9:258/263 of query aligns to 78:338/338 of 3h5nA

query
sites
3h5nA

I

I

L

I

P

P

V

G

Q

E

A

Y

T

N

P

N

P

S

T

T

A

E

E

N

T

N

-

R

-

Y

S

S

R

R

R

N

F

F

D

L

R

H

Q

Y

V

Q

T

S

I

Y

P

G

G

A

F

N

G

P

V

V

A

L

A

V

Q

Q

R

D

R

K

L

L

S

K

Q

N

A

A

T

K

V

V

L

V

V

I

A

L

G

G

V

C

G
|
G

G

G

V

I

G

G

G

N

A

H

A

V

A

S

T

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

R

E

L

I

I

I

L

L

I

I

H
x
D

P

N

G

D

D

Q

L

I

A

E

E

N

P

T

D
x
N

L

L

N

T

R
|
R

Q
|
Q

T

V

L

L

M

F

R

S

P

E

E

D

R

D

V

V

G

G

E

K

P

N

R
x
K

V

T

F

E

C

V

A

I

A

K

D

R

T

E

L

L

R

L

A

K

H

R

H

N

P

S

G

E

V

I

E

S

I

V

V

S

A

E

V

I

D

A

R

L

D
x
N

L
x
I

T

N

D

D

P

Y

A

T

V

D

P

L

G

H

L

K

I

V

A

P

E

E

A

A

D

D

V

I

-

W

V

V

D

S

A
|
A

R
x
D

H
|
H

N

P

S

F

P

N

E

L

R

I

Y

N

L

W

I

V

N

N

R

K

L

Y

C

C

V

V

Q

R

A

A

A

N

V

Q

P

P

L

Y

V

I

V

N

A

A

-

G

A

Y

M

V

N

N

A

D

T

I

E

A

A

V

Y

F

L

G

M

P

V

L

V

Y

R

V

P

P

G

G

E

K

P

T

-

G

C
|
C

L

Y

R

E

C
|
C

V

Q

F

K

G

K

E

E

G

N

-

I

-

D

-

H

-

K

-

I

-

K

-

L

-

I

D

N

P

S

A

R

W

F

E

K

P

P

L

A

G

T

F

F

P

A

V

P

L

V

G

N

A

N

V

V

A

A

G

A

T

A

V

-

G

-

C

-

L

L

A

C

A

A

T

A

E

D

A

V

L

I

K

K

I

F

A

I

G

G

G

K

F

Y

G

S

E

E

-

P

-

L

S

S

A

L

V

N

G

K

R

R

L

-

V

-

H

-

L

I

D

G

L

I

W

W

-

S

-

D

D

E

V

I

D

K

L

I

R

H

T

S

P

Q

R

N

T

M

R

G

R

R

D

S

P

P

A

V

C
|
C

P

S

D

V

C
|
C

G

G

active site: R157 (= R86)
binding adenosine-5'-triphosphate: G124 (= G53), D146 (≠ H75), N154 (≠ D83), R157 (= R86), Q158 (= Q87), K170 (≠ R99), N193 (≠ D122), I194 (≠ L123), A213 (= A141), D214 (≠ R142), H215 (= H143)
binding zinc ion: C257 (= C183), C260 (= C186), C334 (= C254), C337 (= C257)

3h5rA Crystal structure of e. Coli mccb + succinimide (see paper)
25% identity, 95% coverage: 9:258/263 of query aligns to 78:339/340 of 3h5rA

query
sites
3h5rA

I

I

L

I

P

P

V

G

Q

E

A

Y

T

N

P

N

P

S

T

T

A

E

E

N

T

N

-

R

-

Y

S

S

R

R

R

N

F

F

D

L

R

H

Q

Y

V

Q

T

S

I

Y

P

G

G

A

F

N

G

P

V

V

A

L

A

V

Q

Q

R

D

R

K

L

L

S

K

Q

N

A

A

T

K

V

V

L

V

V

I

A

L

G

G

V

C

G

G

V
x
I

G

G

G

N

A

H

A

V

A

S

T

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

R

E

L

I

I

I

L

L

I

I

H

D

P

N

G

D

D

Q

L

I

A

E

E

N

P

T

D

N

L

L

N

T

R
|
R

Q

Q

T

V

L

L

M

F

R

S

P

E

E

D

R

D

V

V

G

G

E

K

P

N

R

K

V

T

F

E

C

V

A

I

A

K

D

R

T

E

L

L

R

L

A

K

H

R

H

N

P

S

G

E

V

I

E

S

I

V

V

S

A

E

V

I

D

A

R

L

D

N

L

I

T

N

D

D

P

Y

A

T

V

D

P

L

G

H

L

K

I

V

A

P

E

E

A

A

D

D

V

I

-

W

V

V

D
x
S

A
|
A

R

D

H

H

N

P

S

F

P

N

E

L

R

I

Y

N

L

W

I

V

N

N

R

K

L

Y

C

C

V

V

Q

R

A

A

A

N

V

Q

P

P

L

Y

V

I

V

N

A
|
A

-
x
G

A
x
Y

M

V

N

N

A

D

T

I

E

A

A
x
V

Y

F

L

G

M

P

V

L

V

Y

R

V

P

P

G

G

E

K

P

T

-

G

C
|
C

L

Y

R

E

C
|
C

-

Q

V

K

F

V

G

K

E

E

G

N

-

I

-

D

-

H

-

K

-

I

-

K

-

L

-

I

D

N

P

S

A

R

W

F

E

K

P
|
P

L

A

G

T

F

F

P

A

V

P

L

V

G

N

A

N

V

V

A

A

G

A

T

A

V

-

G

-

C

-

L

L

A

C

A

A

T

A

E

D

A

V

L

I

K

K

I

F

A

I

G

G

G

K

F

Y

G

S

E

E

-

P

-

L

S

S

A

L

V

N

G

K

R
|
R

L

-

V

-

H

-

L

I

D
x
G

L

I

W

W

-

S

-

D

D

E

V

I

D

K

L

I

R

H

T

S

P

Q

R

N

T

M

R

G

R

R

D

S

P

P

A

V

C
|
C

P

S

D

V

C
|
C

G

G

active site: R157 (= R86)
binding zinc ion: C257 (= C183), C260 (= C186), C335 (= C254), C338 (= C257)
binding : I126 (≠ V55), S212 (≠ D140), A213 (= A141), A237 (= A165), G238 (vs. gap), Y239 (≠ A166), V245 (≠ A172), P280 (= P197), R314 (= R232),