SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_232215828.1 NCBI__GCF_000194625.1:WP_232215828.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

2d6fD Crystal structure of glu-tRNA(gln) amidotransferase in the complex with tRNA(gln) (see paper)
54% identity, 84% coverage: 12:539/626 of query aligns to 2:516/522 of 2d6fD

query
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2d6fD

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binding zinc ion: C28 (= C38), C71 (= C89), E74 (= E92)
binding : Q232 (= Q251), D233 (≠ T252), D235 (= D254), K393 (= K412), R404 (= R423), S453 (≠ P469), D455 (= D471), L456 (= L472), Q459 (≠ V475), R463 (≠ H482), T472 (= T495), S476 (≠ R499), Y480 (≠ I503), R483 (≠ A506), E484 (= E507), R487 (≠ K510)

3h0rH Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
35% identity, 27% coverage: 101:271/626 of query aligns to 51:236/410 of 3h0rH

query
sites
3h0rH

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binding aspartic acid: K79 (= K129), E151 (= E194)
binding adenosine-5'-triphosphate: V153 (≠ G196), T154 (= T197), N195 (= N236), S197 (= S238), R209 (= R244)
binding manganese (ii) ion: E125 (= E167), E151 (= E194), E211 (= E246)

Sites not aligning to the query:

3h0rE Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
35% identity, 27% coverage: 101:271/626 of query aligns to 51:236/410 of 3h0rE

query
sites
3h0rE

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binding adenosine-5'-triphosphate: T154 (= T197), P156 (= P199), N195 (= N236), S197 (= S238), R209 (= R244)
binding manganese (ii) ion: E125 (= E167), E151 (= E194), E211 (= E246)

Sites not aligning to the query:

3h0rB Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
35% identity, 27% coverage: 101:271/626 of query aligns to 51:236/410 of 3h0rB

query
sites
3h0rB

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binding adenosine-5'-diphosphate: T154 (= T197), P156 (= P199), N195 (= N236), S197 (= S238), R209 (= R244)
binding manganese (ii) ion: E125 (= E167), E151 (= E194), N195 (= N236), E211 (= E246)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 8, 9, 10
binding manganese (ii) ion: 10, 12
binding zinc ion: 23, 25, 38, 41

3h0lB Structure of tRNA-dependent amidotransferase gatcab from aquifex aeolicus (see paper)
35% identity, 27% coverage: 101:271/626 of query aligns to 51:236/410 of 3h0lB

query
sites
3h0lB

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N

R

R

L

A

G

G

I

T

P

P

L

L

V

M

E
|
E

I

I

G
x
V

T
|
T

M

E

P
|
P

D

D

I

I

T

R

S

T

P

P

K

E

E

E

A

A

K

R

E

L

V

F

A

L

A

E

K

K

L

L

G

R

M

N

I

I

L

M

R

R

S

Y

T

A

G

G

R

V

V

S

K

K

R

A

G

D

L

M

-

E

-

K

G

G

T

Q

I

L

R

R

Q

C

D

D

V

I

N
|
N

I

V

S
|
S

I

I

R

R

D

P

-

K

-

G

-

S

-

K

-

E

-

F

G

G

A

T

R
|
R

V

V

E

E

I

I

K

K

G

N

V

V

Q

N

T

S

L

F

D

R

I

F

L

V

D

Q

K

K

I

A

V

L

E

E

Y

Y

E

E

V

I

L

E

R

R

Q

Q

L

I

N

N

L

V

L

V

binding adenosine-5'-diphosphate: V153 (≠ G196), T154 (= T197), P156 (= P199), N195 (= N236), S197 (= S238), R209 (= R244)
binding magnesium ion: E125 (= E167), E151 (= E194)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 6, 8, 9, 10
binding magnesium ion: 12
binding zinc ion: 23, 25, 38, 41

3al0B Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
30% identity, 39% coverage: 20:266/626 of query aligns to 7:237/482 of 3al0B

query
sites
3al0B

V

I

G

G

I

L

E

E

I

I

H

H

Q

V

Q

Q

L

L

D

S

T

T

K

K

H

T

K

K

L

A

F

F

C

C

Y

S

C
|
C

P

P

T

A

I

-

Q

-

R

-

D

D

V

V

E

-

E

-

S

-

N

-

F

-

E

-

F

-

F

-

R

-

Y

-

L

-

R

-

S

-

K

-

R

-

S

-

E

-

M

-

G

-

E

-

V

-

D

-

R

-

A

-

A

-

K

-

E

-

E

-

V

-

A

-

R

-

S

-

K

-

K

-

F

F

I

E

Y

L

K

P

A

P

Y

N

D

T

T

A

T

I

C
|
C

L

P

V

V

E
x
C

A

T

D

G

E

Q

E

-

P

-

P

P

R

G

E

A

L

L

-

P

-

V

-

P

N

N

R

E

E

E

A

M

L

I

Q

R

V

F

A

A

I

V

L

K

I

T

A

A

R

L

M

A

L

L

N

N

M

C

E

K

V

I

V

H

D

K

E

Y

V

S

H

R

V

F

M

D

R

R

K

K

I

N

V

Y

I

F

D

Y

G

P

S

D

N

L

T

P

T

K

G

G

F

Y

Q

Q

R

I

T

S

A

Q

L

Y

-

F

-

Y

-

P

I

I

A

A

F

T

D

E

G

G

Y

F

I

L

E

E

C

I

E

D

G

G

-

D

-

E

-

G

-

R

K

K

K

K

I

V

G

R

V

I

A

R

T

R

L

L

C

H

L

L

E

E

E

E

E

D

A

A

C

G

R

K

K

L

V

V

E

H

D

E

-

G

-

D

-

S

-

I

-

T

R

R

G

A

S

S

E

Y

V

S

V

L

Y

V

S

D

L

M

D

N

R

R

L

C

G

G

I

V

P

P

L

L

V

I

E

E

I

I

G

V

T

T

M

E

P

P

D

D

I

I

T

S

S

S

P

P

K

R

E

E

A

A

K

R

E

V

V

F

A

M

A

E

K

K

L

L

G

R

M

S

I

I

L

V

R

R

-

Y

-

L

-

G

-

V

S

S

T

T

G

G

R

D

V

M

K

E

R

K

G

-

L

-

G

G

T

A

I

L

R

R

Q

C

D

D

V

A

N

N

I

I

S

S

I

V

-

V

-

D

-

T

-

E

-

T

-

G

R

R

D

Q

G

S

A

N

R

R

V

V

E

E

I

V

K

K

G

N

V

M

Q

N

T

S

L

F

D
x
R

I

F

L

V

D

E

K

R

I

A

V

L

E

E

Y

Y

E

E

V

F

L

E

R

R

binding zinc ion: C25 (= C38), C39 (= C89), C42 (≠ E92)
binding : R225 (≠ D254)

Sites not aligning to the query:

binding : 319, 349, 352, 353, 356, 357, 391, 394, 439, 442, 449, 452, 453, 455, 456, 468

3ip4B The high resolution structure of gatcab (see paper)
34% identity, 25% coverage: 101:258/626 of query aligns to 51:222/482 of 3ip4B

query
sites
3ip4B

L

V

N

N

R

K

E

R

A

A

L

V

Q

D

V

W

A

A

I

M

L

R

I

A

A

A

R

M

M

A

L

L

N

N

M

M

E

E

V

I

V

A

D

T

E

E

V

S

H

K

V

F

M

D

R

R

K

K

I

N

V

Y

I

F

D

Y

G

P

S

D

N

N

T

P

T

K

G

A

F

Y

Q

Q

R

I

T

S

A

Q

L

F

-

D

-

Q

-

P

I

I

A

G

F

E

D

N

G

G

Y

Y

I

I

E

D

C

I

E

E

-

V

-

D

-

G

-

E

G

T

K

K

K

R

I

I

G

G

V

I

A

T

T

R

L

L

C

H

L

M

E
|
E

E

E

E

D

A

A

C

G

R

K

K

S

V

T

E

H

D

-

R

K

G

G

S

E

E

Y

V

S

V

L

Y

V

S

D

L

L

D

N

R

R

L

Q

G

G

I

T

P

P

L

L

V

I

E
|
E

I

I

G

V

T

S

M

E

P

P

D

D

I

I

T

R

S

S

P

P

K

K

E

E

A

A

K

Y

E

A

V

Y

A

L

A

E

K

K

L

L

G

R

M

S

I

I

L

I

R

Q

S

Y

T

T

G

G

-

V

-

S

R

D

V

V

K

K

R

M

G

E

L

E

G

G

T

S

I

L

R

R

Q

C

D

D

V

A

N

N

I

I

S

S

I

L

R

R

D

P

-

Y

-

G

-

Q

-

E

-

K

-

F

G

G

A

T

R

K

V

A

E

E

I

L

K

K

G

N

V

L

Q

N

T

S

L

F

D

N

I

Y

L

V

D

R

K

K

binding magnesium ion: E124 (= E167), E150 (= E194)

Sites not aligning to the query:

binding magnesium ion: 12

2df4B Structure of tRNA-dependent amidotransferase gatcab complexed with mn2+ (see paper)
34% identity, 25% coverage: 101:258/626 of query aligns to 50:221/399 of 2df4B

query
sites
2df4B

L

V

N

N

R

K

E

R

A

A

L

V

Q

D

V

W

A

A

I

M

L

R

I

A

A

A

R

M

M

A

L

L

N

N

M

M

E

E

V

I

V

A

D

T

E

E

V

S

H

K

V

F

M

D

R

R

K

K

I

N

V

Y

I

F

D

Y

G

P

S

D

N

N

T

P

T

K

G

A

F

Y

Q

Q

R

I

T

S

A

Q

L

F

-

D

-

Q

-

P

I

I

A

G

F

E

D

N

G

G

Y

Y

I

I

E

D

C

I

E

E

-

V

-

D

-

G

-

E

G

T

K

K

K

R

I

I

G

G

V

I

A

T

T

R

L

L

C

H

L

M

E
|
E

E

E

E

D

A

A

C

G

R

K

K

S

V

T

E

H

D

-

R

K

G

G

S

E

E

Y

V

S

V

L

Y

V

S

D

L

L

D

N

R

R

L

Q

G

G

I

T

P

P

L

L

V

I

E
|
E

I

I

G

V

T

S

M

E

P

P

D

D

I

I

T

R

S

S

P

P

K

K

E

E

A

A

K

Y

E

A

V

Y

A

L

A

E

K

K

L

L

G

R

M

S

I

I

L

I

R

Q

S

Y

T

T

G

G

-

V

-

S

R

D

V

V

K

K

R

M

G

E

L

E

G

G

T

S

I

L

R

R

Q

C

D
|
D

V

A

N

N

I

I

S

S

I

L

R

R

D

P

-

Y

-

G

-

Q

-

E

-

K

-

F

G

G

A

T

R

K

V

A

E
|
E

I

L

K

K

G

N

V

L

Q

N

T

S

L

F

D

N

I

Y

L

V

D

R

K

K

binding manganese (ii) ion: E123 (= E167), E149 (= E194), D191 (= D234), E209 (= E246)

Sites not aligning to the query:

binding manganese (ii) ion: 9, 11

2g5iB Structure of tRNA-dependent amidotransferase gatcab complexed with adp-alf4 (see paper)
34% identity, 25% coverage: 101:258/626 of query aligns to 50:221/410 of 2g5iB

query
sites
2g5iB

L

V

N

N

R

K

E

R

A

A

L

V

Q

D

V

W

A

A

I

M

L

R

I

A

A

A

R

M

M

A

L

L

N

N

M

M

E

E

V

I

V

A

D

T

E

E

V

S

H

K

V

F

M

D

R

R

K

K

I

N

V

Y

I

F

D

Y

G

P

S

D

N

N

T

P

T

K

G

A

F

Y

Q

Q

R

I

T

S

A

Q

L

F

-

D

-

Q

-

P

I

I

A

G

F

E

D

N

G

G

Y

Y

I

I

E

D

C

I

E

E

-

V

-

D

-

G

-

E

G

T

K

K

K

R

I

I

G

G

V

I

A

T

T

R

L

L

C

H

L

M

E
|
E

E

E

E

D

A

A

C

G

R

K

K

S

V

T

E

H

D

-

R

K

G

G

S

E

E

Y

V

S

V

L

Y

V

S

D

L

L

D

N

R

R

L

Q

G

G

I

T

P

P

L

L

V

I

E
|
E

I

I

G
x
V

T
x
S

M

E

P
|
P

D

D

I

I

T

R

S

S

P

P

K

K

E

E

A

A

K

Y

E

A

V

Y

A

L

A

E

K

K

L

L

G

R

M

S

I

I

L

I

R

Q

S

Y

T

T

G

G

-

V

-

S

R

D

V

V

K

K

R

M

G

E

L

E

G

G

T

S

I

L

R

R

Q

C

D

D

V

A

N
|
N

I

I

S
|
S

I

L

R

R

D

P

-

Y

-

G

-

Q

-

E

-

K

-

F

G

G

A

T

R
x
K

V

A

E

E

I

L

K

K

G

N

V

L

Q

N

T

S

L

F

D

N

I

Y

L

V

D

R

K

K

binding adenosine-5'-diphosphate: V151 (≠ G196), S152 (≠ T197), P154 (= P199), N193 (= N236), S195 (= S238), K207 (≠ R244)
binding magnesium ion: E123 (= E167), E149 (= E194)

Sites not aligning to the query:

3kfuF Crystal structure of the transamidosome (see paper)
24% identity, 42% coverage: 13:272/626 of query aligns to 2:231/447 of 3kfuF

query
sites
3kfuF

Y

Y

E

E

A

A

L

V

G

-

L

-

K

-

V

I

G

G

I

L

E

E

I

V

H
|
H

Q

L

Q

H

L

L

D

K

T

T

K

R

H

T

K

K

L

M

F

F

C
|
C

Y

G

C
|
C

P

-

T

-

I

-

Q

-

R

-

D

-

V

-

E

-

E

-

S

-

N

-

F

-

E

-

F

-

F

-

R

-

Y

-

L

-

R

-

S

-

K

-

R

-

S

-

E

-

M

-

G

-

E

-

V

-

D

-

R

-

A

-

A

-

K

-

E

-

E

-

V

-

A

-

R

R

S

A

K

D

K

Y

F

F

I

G

Y

A

K

E

A

P

Y

N

D

T

T

H

T

T

C
|
C

-

P

-

V

-

C

-

L

-

G

L

L

V

P

E

G

A

A

D

L

E

P

E

V

P

P

P

-

R

-

E

-

L

-

N

N

R

R

E

V

A

A

L

V

Q

E

V

H

A

G

I

L

L

R

I

L

A

A

R

L

M

A

L

L

N

G

M

A

E

E

V

V

V

P

D

E

E

R

V

L

H

V

V

F

M

H

R

R

K

K

I

N

V
x
Y

I

F

D
x
Y

G

P

S
x
D

N

L

T

P

T

K

G

N

F

Y

Q

Q

R

I

T

S

A

Q

L
x
Y

-
x
D

-

L

-

P

I

L

A

G

F

R

D

G

G

G

Y

S

I

L

E

P

C

L

E

G

G

E

K

R

K

R

I

V

G

R

V

I

A

K

T

R

L

L

C

H

L

L

E
|
E

E

E

E

D

A

A

C

G

R
x
K

K

S

V

L

E
x
H

D

L

R

E

G

G

S

R

E

T

V

L

V

L

Y

-

S

D

L

L

D

N

R
|
R

L

A

G

G

I

S

P

P

L

L

V

I

E
|
E

I

L

G

V

T

T

M

E

P

P

D

D

I

L

T

K

S

T

P

P

K

E

E

E

A

A

K
x
R

E

L

V

F

A

L

A

Q

K

R

L

I

G

Q

M

A

I

L

L

V

R

Q

S

T

T

L

G

G

R

I

V

S

K

D

R

A

G

S

L
x
P

-
x
E

-

E

G
|
G

T

K

I

L

R
|
R

Q

A

D

D

V

V

N

N

I

V

S

S

I

V

R

R

D

R

-

V

-

G

-

E

-

P

-

L

G

G

A

T

R

K

V

V

E

E

I

I

K

K

G

N

V

L

Q
x
N

T
x
S

L
x
F

D
x
K

I

S

L

V

D

Q

K

R

I

A

V

L

E

E

Y

Y

E

E

V

I

L

R

R

R

Q

Q

L

T

N

E

L

I

L

L

K

R

binding magnesium ion: H11 (= H25), E120 (= E167), E146 (= E194)
binding zinc ion: C22 (= C36), C24 (= C38), C38 (= C89)
binding : Y81 (≠ V131), Y83 (≠ D133), D85 (≠ S135), Y95 (≠ L145), D96 (vs. gap), K125 (≠ R172), H128 (≠ E175), R139 (= R187), R160 (≠ K208), P180 (≠ L228), E181 (vs. gap), G183 (= G229), R186 (= R232), N210 (≠ Q251), S211 (≠ T252), F212 (≠ L253), K213 (≠ D254)

Sites not aligning to the query:

binding : 334, 339, 342, 372, 373, 376, 410, 413, 421, 422, 425, 435

4wj3B Crystal structure of the asparagine transamidosome from pseudomonas aeruginosa (see paper)
28% identity, 27% coverage: 101:272/626 of query aligns to 49:235/456 of 4wj3B

query
sites
4wj3B

L

L

N

N

R

E

E

E

A

A

L

V

Q

R

V

M

A

A

I

C

L

L

I

F

A

G

R

L

M

A

L

I

N

D

M

A

E

R

V

I

V

D

D

R

E

Q

V

N

H

V

V

F

M

A

R

R

K

K

I

N

V

Y

I

F

D

Y

G

P

S

D

N

L

T

P

T

K

G

G

F

Y

Q

Q

R

T

T

S

A

Q

L

M

-

D

-

H

-

P

I

I

A

V

F

G

D

K

G

G

Y

H

I

L

E

D

C

I

E

T

-

L

-

E

-

D

-

G

-

T

G

T

K

K

K

R

I

I

G

G

V

I

A

T

T

R

L

A

C

H

L

L

E

E

E

E

E

D

A

A

C

G

R
x
K

K

S

V

L

E

H

D

E

R

D

G

G

S

M

E

S

V

G

V

I

Y

-

S

D

L

L

D

N

R

R

L

A

G

G

I

T

P

P

L

L

V

L

E

E

I

I

G

V

T

S

M

E

P

P

D

D

I

I

T

R

S

S

P

A

K

K

E

E

A

A

K

V

E

A

V

Y

A

V

A

K

K

A

L

I

G

H

M

A

I

L

L

V

R

R

S

Y

T

L

G

G

R

I

V

C

K

D

R

G

G

N

L

M

-
x
A

-

E

G

G

T

S

I

L

R

R

Q

C

D

D

V

C

N

N

I

V

S

S

I

V

R

R

D

P

-

K

-

G

-

Q

-

A

-

E

-
x
F

G

G

A

T

R

R

V

A

E

E

I

I

K

K

G
x
N

V

V

Q

N

T
x
S

L

F

D
x
R

I

F

L

I

D

E

K

K

I

A

V

I

E

N

Y

H

E

E

V

I

L

Q

R

R

Q

Q

L

I

N

E

L

L

L

I

K

E

binding : K128 (≠ R172), A184 (vs. gap), F204 (vs. gap), N212 (≠ G249), S215 (≠ T252), R217 (≠ D254)

Sites not aligning to the query:

binding : 240, 311, 341, 344, 345, 349, 352, 430, 431, 434

Query Sequence

>WP_232215828.1 NCBI__GCF_000194625.1:WP_232215828.1
MANRVNEMNCQDYEALGLKVGIEIHQQLDTKHKLFCYCPTIQRDVEESNFEFFRYLRSKR
SEMGEVDRAAKEEVARSKKFIYKAYDTTCLVEADEEPPRELNREALQVAILIARMLNMEV
VDEVHVMRKIVIDGSNTTGFQRTALIAFDGYIECEGKKIGVATLCLEEEACRKVEDRGSE
VVYSLDRLGIPLVEIGTMPDITSPKEAKEVAAKLGMILRSTGRVKRGLGTIRQDVNISIR
DGARVEIKGVQTLDILDKIVEYEVLRQLNLLKIRDELLARGASVVNQIFDVTSIFSNTKS
KVIKRAKCVKAILLKGFAGLVGKEIQPGRRLGTEFADIAKTFGLGGIFHTDELPAYGISE
EEVAELRRFVGAESNDAVILAAGEATRVENALARIIERAEYCLIGVPEETRKANEDGTTS
YLRPLPGAARMYPETDVPAVRITPDMLEVEIPELIEERAKRYAETYGLPYDLAYVMADSA
LHHLFEEFAEKLPATTVARVLHIAPAELKKEGVEIGKLEEGHFRAVLELIEEGKIAKEGA
EEALKIFCEKPTATKEEVVSSIGSATNLDSFIAKLMEEKKDFIKERGASAFKPLMGLVMK
EFRGKVDGKVVAEKLNEAIKRALSDG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory