SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_232422309.1 NCBI__GCF_000372865.1:WP_232422309.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6udeB Crystal structure of glycerol kinase from elizabethkingia anophelis nuhp1 in complex with adp and glycerol
53% identity, 100% coverage: 1:490/490 of query aligns to 6:494/495 of 6udeB

query
sites
6udeB

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binding adenosine-5'-diphosphate: R16 (= R11), G262 (= G259), T263 (= T260), G306 (= G303), I309 (= I306), S323 (≠ A320), G406 (= G403), G407 (= G404), A408 (= A405)
binding magnesium ion: G11 (= G6), T12 (= T7), T13 (≠ S8), S14 (= S9)

1gllO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
52% identity, 100% coverage: 1:490/490 of query aligns to 6:493/494 of 1gllO

query
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1gllO

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binding phosphomethylphosphonic acid adenylate ester: T12 (= T7), T13 (≠ S8), G261 (= G259), T262 (= T260), G305 (= G303), I308 (= I306), Q309 (= Q307), A321 (≠ T319), G406 (= G404), N410 (= N408)
binding glycerol: R82 (= R77), E83 (= E78), Y134 (= Y129), D240 (= D238), Q241 (= Q239), F265 (= F263)

1gljO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
52% identity, 100% coverage: 1:490/490 of query aligns to 6:493/494 of 1gljO

query
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1gljO

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S

S

K

E

H

N

R

G

L

L

L

L

T

T

T

T

I

I

A

A

W

C

Q

G

I

P

G

T

D

G

E

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

T

M

G

A

G
|
G

S

A

V

S

I

I

Q
|
Q

W

W

L

L

R

R

D

D

G

E

L

M

R

K

L

L

F

I

D

N

R

D

T
x
A

A

Y

D

D

C

S

E

E

A

Y

L

F

A

A

G

T

S

K

V

V

P

Q

D

N

S

T

G

N

G

G

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

T

G

G

L

L

G

G

A

A

P

P

H

Y

W

W

D

D

P

P

R

Y

A

A

R

R

G

G

S

A

L

I

F

F

G

G

I

L

T

T

G

R

G

G

T

V

T

N

G

A

A

N

H

H

I

I

A

I

R

R

A

A

A

T

I

L

E

E

S

S

I

I

C

A

F

Y

Q

Q

V

T

N

R

D

D

L

V

L

L

N

E

A

A

L

M

S

Q

E

A

D

D

G

S

G

G

R

I

P

R

V

L

P

H

Q

A

L

L

R

R

I

V

D

D

G

G

G
|
G

A
|
A

A

V

T

A

N

N

N

N

L

F

L

L

A

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

S

L

S

G

L

T

E

R

V

V

V

E

R

R

P

P

Q

E

T

V

L

R

E

E

S

V

T

T

A

A

L

L

G

G

A

A

A

A

C

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

Q

N

N

L

L

E

D

E

E

L

L

R

Q

K

E

Q

K

W

A

A

V

I

I

D

E

R

R

T

E

F

F

H

R

G

P

A

G

M

I

P

E

P

T

E

T

E

E

I

R

D

N

R

Y

R

R

T

Y

R

A

R

G

W

W

L

K

K

K

A

A

V

V

E

K

R

R

A

A

K

M

N

A

W

W

A

E

D

E

binding gamma-arsono-beta, gamma-methyleneadenosine-5'-diphosphate: T12 (= T7), T13 (≠ S8), G261 (= G259), T262 (= T260), G305 (= G303), Q309 (= Q307), A321 (≠ T319), G406 (= G404), A407 (= A405)
binding glycerol: R82 (= R77), E83 (= E78), W102 (= W97), Y134 (= Y129), D240 (= D238), F265 (= F263)

1bwfO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
52% identity, 100% coverage: 1:490/490 of query aligns to 6:493/494 of 1bwfO

query
sites
1bwfO

M

V

A

A

I

L

D

D

Q

Q

G

G

T
|
T

S
x
T

S

S

S

S

R

R

C

A

I

V

I

V

Y

M

D

D

R

H

R

D

A

A

N

N

P

I

V

I

A

S

M

V

A

S

Q

Q

Q

R

P

E

L

F

K

E

S

Q

C

I

Y

Y

P

P

E

K

P

P

G

G

W

W

V

V

E

E

Q

H

D

D

A

P

E

M

D

E

I

I

W

W

R

A

T

T

Q

Q

A

S

E

W

T

T

I

L

R

V

L

E

A

V

L

L

S

A

Q

K

R

A

Q

D

L

I

Q

S

A

S

S

D

D

Q

L

I

I

A

A

A

V

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

V

T

V

I

V

V

W

W

D

E

R

K

L

E

T

T

G

G

K

K

P

P

I

I

H

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

D

C

R

R

R

R

T

T

A

A

E

E

E

I

C

C

R

E

R

H

L

L

Q

K

E

R

A

D

G

G

L

L

E

E

P

D

M

Y

I

I

R

R

A

S

K

N

T

T

G

G

L

L

L

V

I

I

D

D

P

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

R

K

W

W

I

I

L

L

D

D

H

H

V

V

E

E

G

G

A

S

R

R

E

E

K

R

A

A

G

R

R

R

G

G

E

E

L

L

A

L

C

F

G

G

T

T

V

V

D

D

S

T

W

W

L

L

V

I

W

W

N

K

L

M

T

T

G

Q

G

G

A

R

L

V

H

H

I

V

T

T

D

D

V

Y

S

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

Y

F

N

N

I

I

E

H

S

T

R

L

Q

D

W

W

D

D

E

D

E

K

L

M

L

L

N

E

L

V

F

L

E

D

I

I

P

P

S

R

S

E

L

M

L

L

P

P

E

E

V

V

K

R

S

R

T

S

A

S

E

E

I

V

Y

Y

G

G

T

Q

T

T

A

-

H

-

P

-

G

N

I

I

G

G

S

T

G

R

I

I

P

P

I

I

A

S

A

G

V

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

L

V

D

K

P

E

G

G

M

M

A

A

K

K

C

N

T

T

Y

Y

G

G

T
|
T

G

G

S

C

F
|
F

L

M

M

L

M

M

N

N

T

T

G

G

P

E

E

K

R

A

R

V

A

K

S

S

K

E

H

N

R

G

L

L

L

L

T

T

T

T

I

I

A

A

W

C

Q

G

I

P

G

T

D

G

E

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

T

M

G

A

G
|
G

S

A

V

S

I
|
I

Q
|
Q

W

W

L

L

R

R

D

D

G

E

L

M

R

K

L

L

F

I

D

N

R

D

T
x
A

A

Y

D

D

C

S

E

E

A

Y

L

F

A

A

G

T

S

K

V

V

P

Q

D

N

S

T

G

N

G

G

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

T

G

G

L

L

G

G

A

A

P

P

H

Y

W

W

D

D

P

P

R

Y

A

A

R

R

G

G

S

A

L

I

F

F

G

G

I

L

T

T

G

R

G

G

T

V

T

N

G

A

A

N

H

H

I

I

A

I

R

R

A

A

A

T

I

L

E

E

S

S

I

I

C

A

F

Y

Q

Q

V

T

N

R

D

D

L

V

L

L

N

E

A

A

L

M

S

Q

E

A

D

D

G

S

G

G

R

I

P

R

V

L

P

H

Q

A

L

L

R

R

I

V

D

D

G

G

G
|
G

A

A

A

V

T

A

N
|
N

N

N

L

F

L

L

A

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

S

L

S

G

L

T

E

R

V

V

V

E

R

R

P

P

Q

E

T

V

L

R

E

E

S

V

T

T

A

A

L

L

G

G

A

A

A

A

C

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

Q

N

N

L

L

E

D

E

E

L

L

R

Q

K

E

Q

K

W

A

A

V

I

I

D

E

R

R

T

E

F

F

H

R

G

P

A

G

M

I

P

E

P

T

E

T

E

E

I

R

D

N

R

Y

R

R

T

Y

R

A

R

G

W

W

L

K

K

K

A

A

V

V

E

K

R

R

A

A

K

M

N

A

W

W

A

E

D

E

binding phosphodifluoromethylphosphonic acid-adenylate ester: T12 (= T7), T13 (≠ S8), T262 (= T260), G305 (= G303), I308 (= I306), Q309 (= Q307), A321 (≠ T319), G406 (= G404), N410 (= N408)
binding glycerol: R82 (= R77), E83 (= E78), W102 (= W97), Y134 (= Y129), D240 (= D238), Q241 (= Q239), F265 (= F263)

1glfO Crystal structures of escherichia coli glycerol kinase and the mutant a65t in an inactive tetramer: conformational changes and implications for allosteric regulation (see paper)
52% identity, 100% coverage: 1:490/490 of query aligns to 6:497/498 of 1glfO

query
sites
1glfO

M

V

A

A

I

L

D

D

Q

Q

G

G

T

T

S

T

S

S

S

S

R
|
R

C

A

I

V

I

V

Y

M

D

D

R

H

R

D

A

A

N

N

P

I

V

I

A

S

M

V

A

S

Q

Q

Q

R

P

E

L

F

K

E

S

Q

C

I

Y

Y

P

P

E

K

P

P

G

G

W

W

V

V

E

E

Q

H

D

D

A

P

E

M

D

E

I

I

W

W

R

A

T

T

Q

Q

A

S

E

S

T

T

I

L

R

V

L

E

A

V

L

L

S

A

Q

K

R

A

Q

D

L

I

Q

S

A

S

S

D

D

Q

L

I

I

A

A

A

V

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

V

T

V

I

V

V

W

W

D

E

R

K

L

E

T

T

G

G

K

K

P

P

I

I

H

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

D

C

R

R

R

R

T

T

A

A

E

E

E

I

C

C

R

E

R

H

L

L

Q

K

E

R

A

D

G

G

L

L

E

E

P

D

M

Y

I

I

R

R

A

S

K

N

T

T

G

G

L

L

L

V

I

I

D

D

P

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

R

K

W

W

I

I

L

L

D

D

H

H

V

V

E

E

G

G

A

S

R

R

E

E

K

R

A

A

G

R

R

R

G

G

E

E

L

L

A

L

C

F

G

G

T

T

V

V

D

D

S

T

W

W

L

L

V

I

W

W

N

K

L

M

T

T

G

Q

G

G

A

R

L

V

H

H

I

V

T

T

D

D

V

Y

S

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

Y

F

N

N

I

I

E

H

S

T

R

L

Q

D

W

W

D

D

E

D

E

K

L

M

L

L

N

E

L

V

F

L

E

D

I

I

P

P

S

R

S

E

L

M

L

L

P

P

E

E

V

V

K

R

S

R

T

S

A

S

E

E

I

V

Y

Y

G

G

T

Q

T

T

A

N

H

I

P

G

G

G

I

K

-
x
G

G
|
G

S

T

G
x
R

I

I

P

P

I

I

A

S

A

G

V

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

L

V

D

K

P

E

G

G

M

M

A

A

K

K

C

N

T

T

Y

Y

G
|
G

T
|
T

G

G

S

C

F

F

L

M

M

L

M

M

N

N

T

T

G

G

P

E

E

K

R

A

R

V

A

K

S

S

K

E

H

N

R

G

L

L

L

L

T

T

T

T

I

I

A

A

W

C

Q

G

I

P

G

T

D

G

E

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

T

M

G

A

G
|
G

S

A

V

S

I

I

Q

Q

W

W

L

L

R

R

D

D

G

E

L

M

R

K

L

L

F

I

D

N

R

D

T

A

A

Y

D

D

C

S

E

E

A

Y

L

F

A

A

G

T

S

K

V

V

P

Q

D

N

S

T

G

N

G

G

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

T

G

G

L

L

G

G

A

A

P

P

H

Y

W

W

D

D

P

P

R

Y

A

A

R

R

G

G

S

A

L

I

F

F

G

G

I

L

T

T

G

R

G

G

T

V

T

N

G

A

A

N

H

H

I

I

A

I

R

R

A

A

A

T

I

L

E

E

S

S

I

I

C

A

F

Y

Q

Q

V

T

N

R

D

D

L

V

L

L

N

E

A

A

L

M

S

Q

E

A

D

D

G

S

G

G

R

I

P

R

V

L

P

H

Q

A

L

L

R

R

I

V

D

D

G

G

G
|
G

A
|
A

A

V

T

A

N

N

N

N

L

F

L

L

A

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

S

L

S

G

L

T

E

R

V

V

V

E

R

R

P

P

Q

E

T

V

L

R

E

E

S

V

T

T

A

A

L

L

G

G

A

A

A

A

C

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

Q

N

N

L

L

E

D

E

E

L

L

R

Q

K

E

Q

K

W

A

A

V

I

I

D

E

R

R

T

E

F

F

H

R

G

P

A

G

M

I

P

E

P

T

E

T

E

E

I

R

D

N

R

Y

R

R

T

Y

R

A

R

G

W

W

L

K

K

K

A

A

V

V

E

K

R

R

A

A

K

M

N

A

W

W

A

E

D

E

binding adenosine-5'-diphosphate: R16 (= R11), G265 (= G259), T266 (= T260), G309 (= G303), G410 (= G404), A411 (= A405)
binding glycerol: R82 (= R77), E83 (= E78), Y134 (= Y129), D244 (= D238)
binding phosphate ion: G232 (vs. gap), G233 (= G227), R235 (≠ G229)

1bo5O Crystal structure of the complex between escherichia coli glycerol kinase and the allosteric regulator fructose 1,6-bisphosphate. (see paper)
52% identity, 100% coverage: 1:490/490 of query aligns to 6:497/498 of 1bo5O

query
sites
1bo5O

M

V

A

A

I

L

D

D

Q

Q

G

G

T

T

S

T

S

S

S

S

R

R

C

A

I

V

I

V

Y

M

D

D

R

H

R

D

A

A

N

N

P

I

V

I

A

S

M

V

A

S

Q

Q

Q

R

P

E

L

F

K

E

S

Q

C

I

Y

Y

P

P

E

K

P

P

G

G

W

W

V

V

E

E

Q

H

D

D

A

P

E

M

D

E

I

I

W

W

R

A

T

T

Q

Q

A

S

E

S

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binding 1,6-di-O-phosphono-beta-D-fructofuranose: G232 (vs. gap), G233 (= G227), R235 (≠ G229)
binding glycerol: R82 (= R77), E83 (= E78), W102 (= W97), Y134 (= Y129), D244 (= D238), Q245 (= Q239), F269 (= F263)

P0A6F3 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Escherichia coli (strain K12) (see 10 papers)
52% identity, 100% coverage: 1:490/490 of query aligns to 8:499/502 of P0A6F3

query
sites
P0A6F3

M

V

A

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W

-

Q

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N

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M
x
I

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E
|
E

I
x
R

D

N

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Y

R

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Y

R

A

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G

W

W

L

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T14 (= T7) binding ADP; binding sn-glycerol 3-phosphate
R18 (= R11) binding ADP
S59 (≠ E52) mutation to W: Abolishes inhibition of GK by FBP via disruption of the dimer-tetramer assembly reaction. Inhibition by EIIA-Glc is unchanged compared to wild type. The activity of this mutant is significantly higher than wild-type, and the Michaelis constants are increased slightly compared to wild-type.
A66 (≠ S59) mutation to T: Although it completely abolishes FBP regulation and disrupts dimer-tetramer equilibrium, the crystal structure is essentially identical to the symmetric tetramer found in the FBP-bound form of the enzyme.
R84 (= R77) binding glycerol; binding sn-glycerol 3-phosphate
E85 (= E78) binding glycerol; binding sn-glycerol 3-phosphate
Y136 (= Y129) binding glycerol; binding sn-glycerol 3-phosphate
G231 (≠ P224) mutation to D: Displays an increased enzymatic activity and a decreased allosteric regulation by FBP compared to wild-type. It displays a dimer form and is resistant to tetramer formation in the presence of FBP, whereas wild-type dimers are converted into inactive tetramers in the presence of FBP.
K233 (≠ I226) modified: N6-malonyllysine
G235 (= G227) binding beta-D-fructose 1,6-bisphosphate
R237 (≠ G229) binding beta-D-fructose 1,6-bisphosphate; mutation to A: Drastically reduces inhibition of GK by FBP and lowers, but did not eliminate, the ability of FBP to promote tetramer association.
D246 (= D238) binding glycerol; binding sn-glycerol 3-phosphate
Q247 (= Q239) binding glycerol
T268 (= T260) binding ADP
G305 (= G297) mutation to S: In glpK22; abolishes glucose control of glycerol utilization.
G311 (= G303) binding ADP
G412 (= G404) binding ADP
N416 (= N408) binding ADP
I475 (≠ M466) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. It increases the affinity for FBP about fivefold.
E479 (= E470) binding Zn(2+)
R480 (≠ I471) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. Regulation by FBP is not affected by this substitution. No inhibition by EIIA-Glc is observed, which is consistent with a decrease in affinity for EIIA-Glc of about 250-fold.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1bu6Y Crystal structures of escherichia coli glycerol kinase and the mutant a65t in an inactive tetramer: conformational changes and implications for allosteric regulation (see paper)
52% identity, 100% coverage: 1:490/490 of query aligns to 6:497/499 of 1bu6Y

query
sites
1bu6Y

M

V

A

A

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D

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A

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D

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A

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G

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N

N

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E

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W

D

E

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Y

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W

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V

D

K

P

E

G

G

M

M

A

A

K

K

C

N

T

T

Y

Y

G

G

T

T

G

G

S

C

F

F

L

M

M

L

M

M

N

N

T

T

G

G

P

E

E

K

R

A

R

V

A

K

S

S

K

E

H

N

R

G

L

L

L

L

T

T

T

T

I

I

A

A

W

C

Q

G

I

P

G

T

D

G

E

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

T

M

G

A

G

G

S

A

V

S

I

I

Q

Q

W

W

L

L

R

R

D

D

G

E

L

M

R

K

L

L

F

I

D

N

R

D

T

A

A

Y

D

D

C

S

E

E

A

Y

L

F

A

A

G

T

S

K

V

V

P

Q

D

N

S

T

G

N

G

G

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

T

G

G

L

L

G

G

A

A

P

P

H

Y

W

W

D

D

P

P

R

Y

A

A

R

R

G

G

S

A

L

I

F

F

G

G

I

L

T

T

G

R

G

G

T

V

T

N

G

A

A

N

H

H

I

I

A

I

R

R

A

A

A

T

I

L

E

E

S

S

I

I

C

A

F

Y

Q

Q

V

T

N

R

D

D

L

V

L

L

N

E

A

A

L

M

S

Q

E

A

D

D

G

S

G

G

R

I

P

R

V

L

P

H

Q

A

L

L

R

R

I

V

D

D

G

G

G

G

A

A

A

V

T

A

N

N

N

N

L

F

L

L

A

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

S

L

S

G

L

T

E

R

V

V

V

E

R

R

P

P

Q

E

T

V

L

R

E

E

S

V

T

T

A

A

L

L

G

G

A

A

A

A

C

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

Q

N

N

L

L

E

D

E

E

L

L

R

Q

K

E

Q

K

W

A

A

V

I

I

D

E

R

R

T

E

F

F

H

R

G

P

A

G

M

I

P

E

P

T

E

T

E

E

I

R

D

N

R

Y

R

R

T

Y

R

A

R

G

W

W

L

K

K

K

A

A

V

V

E

K

R

R

A

A

K

M

N

A

W

W

A

E

D

E

binding glycerol: Q81 (= Q76), R82 (= R77), E83 (= E78), W102 (= W97), Y134 (= Y129), D244 (= D238)

1gldG Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
52% identity, 100% coverage: 1:490/490 of query aligns to 4:488/489 of 1gldG

query
sites
1gldG

M

V

A

A

I

L

D
|
D

Q

Q

G

G

T
|
T

S

T

S

S

S

S

R
|
R

C

A

I

V

I

V

Y

M

D

D

R

H

R

D

A

A

N

N

P

I

V

I

A

S

M

V

A

S

Q

Q

Q

R

P

E

L

F

K

E

S

Q

C

I

Y

Y

P

P

E

K

P

P

G

G

W

W

V

V

E

E

Q

H

D

D

A

P

E

M

D

E

I

I

W

W

R

A

T

T

Q

Q

A

S

E

S

T

T

I

L

R

V

L

E

A

V

L

L

S

A

Q

K

R

A

Q

D

L

I

Q

S

A

S

S

D

D

Q

L

I

I

A

A

A

V

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

V

T

V

I

V

V

W

W

D

E

R

K

L

E

T

T

G

G

K

K

P

P

I

I

H

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

D

C

R

R

R

R

T

T

A

A

E

E

E

I

C

C

R

E

R

H

L

L

Q

K

E

R

A

D

G

G

L

L

E

E

P

D

M

Y

I

I

R

R

A

S

K

N

T

T

G

G

L

L

L

V

I

I

D

D

P

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

R

K

W

W

I

I

L

L

D

D

H

H

V

V

E

E

G

G

A

S

R

R

E

E

K

R

A

A

G

R

R

R

G

G

E

E

L

L

A

L

C

F

G

G

T

T

V

V

D

D

S

T

W

W

L

L

V

I

W

W

N

K

L

M

T

T

G

Q

G

G

A

R

L

V

H

H

I

V

T

T

D

D

V

Y

S

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

Y

F

N

N

I

I

E

H

S

T

R

L

Q

D

W

W

D

D

E

D

E

K

L

M

L

L

N

E

L

V

F

L

E

D

I

I

P

P

S

R

S

E

L

M

L

L

P

P

E

E

V

V

K

R

S

R

T

S

A

S

E

E

I

V

Y

Y

G

G

T

Q

T

T

A

-

H

-

P

-

G

-

I

-

G

-

S

N

G

I

I

I

P

P

I

I

A

S

A

G

V

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

L

V

D

K

P

E

G

G

M

M

A

A

K

K

C

N

T

T

Y

Y

G
|
G

T
|
T

G

G

S

C

F
|
F

L

M

M

L

M

M

N

N

T

T

G

G

P

E

E

K

R

A

R

V

A

K

S

S

K

E

H

N

R

G

L

L

L

L

T

T

T

T

I

I

A

A

W

C

Q

G

I

P

G

T

D

G

E

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

T

M

G

A

G
|
G

S

A

V

S

I

I

Q

Q

W

W

L

L

R

R

D

D

G

E

L

M

R

K

L

L

F

I

D

N

R

D

T
x
A

A

Y

D

D

C

S

E

E

A

Y

L

F

A

A

G

T

S

K

V

V

P

Q

D

N

S

T

G

N

G

G

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

T

G

G

L

L

G

G

A

A

P

P

H

Y

W

W

D

D

P

P

R

Y

A

A

R

R

G

G

S

A

L

I

F

F

G

G

I

L

T

T

G

R

G

G

T

V

T

N

G

A

A

N

H

H

I

I

A

I

R

R

A

A

A

T

I

L

E

E

S

S

I

I

C

A

F

Y

Q

Q

V

T

N

R

D

D

L

V

L

L

N

E

A

A

L

M

S

Q

E

A

D

D

G

S

G

G

R

I

P

R

V

L

P

H

Q

A

L

L

R

R

I

V

D

D

G

G

G
|
G

A
|
A

A

V

T

A

N
|
N

N

N

L

F

L

L

A

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

S

L

S

G

L

T

E

R

V

V

V

E

R

R

P

P

Q

E

T

V

L

R

E

E

S

V

T

T

A

A

L

L

G

G

A

A

A

A

C

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

Q

N

N

L

L

E

D

E

E

L

L

R

Q

K

E

Q

K

W

A

A

V

I

I

D

E

R

R

T

E

F

F

H

R

G

P

A

G

M

I

P

E

P

T

E

T

E

E

I

R

D

N

R

Y

R

R

T

Y

R

A

R

G

W

W

L

K

K

K

A

A

V

V

E

K

R

R

A

A

K

M

N

A

W

W

A

E

D

E

binding adenosine-5'-diphosphate: R14 (= R11), G256 (= G259), T257 (= T260), G300 (= G303), A316 (≠ T319), G401 (= G404), A402 (= A405), N405 (= N408)
binding glyceraldehyde-3-phosphate: T10 (= T7), R80 (= R77), E81 (= E78), Y132 (= Y129), D235 (= D238), F260 (= F263)
binding manganese (ii) ion: D7 (= D4), R14 (= R11)

1glcG Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
52% identity, 100% coverage: 1:490/490 of query aligns to 4:488/489 of 1glcG

query
sites
1glcG

M

V

A

A

I

L

D

D

Q

Q

G

G

T
|
T

S

T

S

S

S

S

R

R

C

A

I

V

I

V

Y

M

D

D

R

H

R

D

A

A

N

N

P

I

V

I

A

S

M

V

A

S

Q

Q

Q

R

P

E

L

F

K

E

S

Q

C

I

Y

Y

P

P

E

K

P

P

G

G

W

W

V

V

E

E

Q

H

D

D

A

P

E

M

D

E

I

I

W

W

R

A

T

T

Q

Q

A

S

E

S

T

T

I

L

R

V

L

E

A

V

L

L

S

A

Q

K

R

A

Q

D

L

I

Q

S

A

S

S

D

D

Q

L

I

I

A

A

A

V

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

V

T

V

I

V

V

W

W

D

E

R

K

L

E

T

T

G

G

K

K

P

P

I

I

H

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

D

C

R

R

R

R

T

T

A

A

E

E

E

I

C

C

R

E

R

H

L

L

Q

K

E

R

A

D

G

G

L

L

E

E

P

D

M

Y

I

I

R

R

A

S

K

N

T

T

G

G

L

L

L

V

I

I

D

D

P

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

R

K

W

W

I

I

L

L

D

D

H

H

V

V

E

E

G

G

A

S

R

R

E

E

K

R

A

A

G

R

R

R

G

G

E

E

L

L

A

L

C

F

G

G

T

T

V

V

D

D

S

T

W

W

L

L

V

I

W

W

N

K

L

M

T

T

G

Q

G

G

A

R

L

V

H

H

I

V

T

T

D

D

V

Y

S

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

Y

F

N

N

I

I

E

H

S

T

R

L

Q

D

W

W

D

D

E

D

E

K

L

M

L

L

N

E

L

V

F

L

E

D

I

I

P

P

S

R

S

E

L

M

L

L

P

P

E

E

V

V

K

R

S

R

T

S

A

S

E

E

I

V

Y

Y

G

G

T

Q

T

T

A

-

H

-

P

-

G

-

I

-

G

-

S

N

G

I

I

I

P

P

I

I

A

S

A

G

V

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

L

V

D

K

P

E

G

G

M

M

A

A

K

K

C

N

T

T

Y

Y

G
|
G

T
|
T

G

G

S

C

F
|
F

L

M

M

L

M

M

N

N

T

T

G

G

P

E

E

K

R

A

R

V

A

K

S

S

K

E

H

N

R

G

L

L

L

L

T

T

T

T

I

I

A

A

W

C

Q

G

I

P

G

T

D

G

E

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

T

M

G

A

G
|
G

S

A

V

S

I

I

Q

Q

W

W

L

L

R

R

D

D

G

E

L

M

R

K

L

L

F

I

D

N

R

D

T
x
A

A

Y

D

D

C

S

E

E

A

Y

L

F

A

A

G

T

S

K

V

V

P

Q

D

N

S

T

G

N

G

G

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

T

G

G

L

L

G

G

A

A

P

P

H

Y

W

W

D

D

P

P

R

Y

A

A

R

R

G

G

S

A

L

I

F

F

G

G

I

L

T

T

G

R

G

G

T

V

T

N

G

A

A

N

H

H

I

I

A

I

R

R

A

A

A

T

I

L

E

E

S

S

I

I

C

A

F

Y

Q

Q

V

T

N

R

D

D

L

V

L

L

N

E

A

A

L

M

S

Q

E

A

D

D

G

S

G

G

R

I

P

R

V

L

P

H

Q

A

L

L

R

R

I

V

D

D

G

G

G
|
G

A
|
A

A

V

T

A

N
|
N

N

N

L

F

L

L

A

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

S

L

S

G

L

T

E

R

V

V

V

E

R

R

P

P

Q

E

T

V

L

R

E

E

S

V

T

T

A

A

L

L

G

G

A

A

A

A

C

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

Q

N

N

L

L

E

D

E

E

L

L

R

Q

K

E

Q

K

W

A

A

V

I

I

D

E

R

R

T

E

F

F

H

R

G

P

A

G

M

I

P

E

P

T

E

T

E

E

I

R

D

N

R

Y

R

R

T

Y

R

A

R

G

W

W

L

K

K

K

A

A

V

V

E

K

R

R

A

A

K

M

N

A

W

W

A

E

D

E

binding adenosine-5'-diphosphate: G256 (= G259), T257 (= T260), G300 (= G303), A316 (≠ T319), G401 (= G404), A402 (= A405), N405 (= N408)
binding glyceraldehyde-3-phosphate: T10 (= T7), R80 (= R77), E81 (= E78), W100 (= W97), Y132 (= Y129), D235 (= D238), F260 (= F263)

1glbG Structure of the regulatory complex of escherichia coli iiiglc with glycerol kinase (see paper)
52% identity, 100% coverage: 1:490/490 of query aligns to 4:488/489 of 1glbG

query
sites
1glbG

M

V

A

A

I

L

D

D

Q

Q

G

G

T

T

S

T

S

S

S

S

R
|
R

C

A

I

V

I

V

Y

M

D

D

R

H

R

D

A

A

N

N

P

I

V

I

A

S

M

V

A

S

Q

Q

Q

R

P

E

L

F

K

E

S

Q

C

I

Y

Y

P

P

E

K

P

P

G

G

W

W

V

V

E

E

Q

H

D

D

A

P

E

M

D

E

I

I

W

W

R

A

T

T

Q

Q

A

S

E

S

T

T

I

L

R

V

L

E

A

V

L

L

S

A

Q

K

R

A

Q

D

L

I

Q

S

A

S

S

D

D

Q

L

I

I

A

A

A

V

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

V

T

V

I

V

V

W

W

D

E

R

K

L

E

T

T

G

G

K

K

P

P

I

I

H

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

D

C

R

R

R

R

T

T

A

A

E

E

E

I

C

C

R

E

R

H

L

L

Q

K

E

R

A

D

G

G

L

L

E

E

P

D

M

Y

I

I

R

R

A

S

K

N

T

T

G

G

L

L

L

V

I

I

D

D

P

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

R

K

W

W

I

I

L

L

D

D

H

H

V

V

E

E

G

G

A

S

R

R

E

E

K

R

A

A

G

R

R

R

G

G

E

E

L

L

A

L

C

F

G

G

T

T

V

V

D

D

S

T

W

W

L

L

V

I

W

W

N

K

L

M

T

T

G

Q

G

G

A

R

L

V

H

H

I

V

T

T

D

D

V

Y

S

T

N

N

A

A

A

S

R

R

T

T

L

M

L

L

Y

F

N

N

I

I

E

H

S

T

R

L

Q

D

W

W

D

D

E

D

E

K

L

M

L

L

N

E

L

V

F

L

E

D

I

I

P

P

S

R

S

E

L

M

L

L

P

P

E

E

V

V

K

R

S

R

T

S

A

S

E

E

I

V

Y

Y

G

G

T

Q

T

T

A

-

H

-

P

-

G

-

I

-

G

-

S

N

G

I

I

I

P

P

I

I

A

S

A

G

V

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

L

C

C

L

V

D

K

P

E

G

G

M

M

A

A

K

K

C

N

T

T

Y

Y

G
|
G

T
|
T

G

G

S

C

F
|
F

L

M

M

L

M

M

N

N

T

T

G

G

P

E

E

K

R

A

R

V

A

K

S

S

K

E

H

N

R

G

L

L

L

L

T

T

T

T

I

I

A

A

W

C

Q

G

I

P

G

T

D

G

E

E

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

S

A

V

V

F

F

T

M

G

A

G
|
G

S

A

V

S

I
|
I

Q

Q

W

W

L

L

R

R

D

D

G

E

L

M

R

K

L

L

F

I

D

N

R

D

T
x
A

A

Y

D

D

C

S

E

E

A

Y

L

F

A

A

G

T

S

K

V

V

P

Q

D

N

S

T

G

N

G

G

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

T

G

G

L

L

G

G

A

A

P

P

H

Y

W

W

D

D

P

P

R

Y

A

A

R

R

G

G

S

A

L

I

F

F

G

G

I

L

T

T

G

R

G

G

T

V

T

N

G

A

A

N

H

H

I

I

A

I

R

R

A

A

A

T

I

L

E

E

S

S

I

I

C

A

F

Y

Q

Q

V

T

N

R

D

D

L

V

L

L

N

E

A

A

L

M

S

Q

E

A

D

D

G

S

G

G

R

I

P

R

V

L

P

H

Q

A

L

L

R

R

I

V

D

D

G

G

G
|
G

A
|
A

A

V

T

A

N
|
N

N

N

L

F

L

L

A

M

Q

Q

F

F

Q

Q

A

S

D

D

I

I

S

L

S

G

L

T

E

R

V

V

V

E

R

R

P

P

Q

E

T

V

L

R

E

E

S

V

T

T

A

A

L

L

G

G

A

A

A

A

C

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

Q

N

N

L

L

E

D

E

E

L

L

R

Q

K

E

Q

K

W

A

A

V

I

I

D

E

R

R

T

E

F

F

H

R

G

P

A

G

M

I

P

E

P

T

E

T

E

E

I

R

D

N

R

Y

R

R

T

Y

R

A

R

G

W

W

L

K

K

K

A

A

V

V

E

K

R

R

A

A

K

M

N

A

W

W

A

E

D

E

binding adenosine-5'-diphosphate: R14 (= R11), G256 (= G259), T257 (= T260), G300 (= G303), I303 (= I306), A316 (≠ T319), G401 (= G404), A402 (= A405), N405 (= N408)
binding glycerol: R80 (= R77), E81 (= E78), W100 (= W97), Y132 (= Y129), D235 (= D238), F260 (= F263)

P18157 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Bacillus subtilis (strain 168) (see paper)
51% identity, 99% coverage: 1:485/490 of query aligns to 6:492/496 of P18157

query
sites
P18157

M

L

A

S

I

L

D

D

Q

Q

G

G

T

T

S

T

S

S

S

S

R

R

C

A

I

I

I

L

Y

F

D

N

R

K

R

E

A

G

N

K

P

I

V

V

A

H

M

S

A

A

Q

Q

Q

K

P

E

L

F

K

T

S

Q

C

Y

Y

F

P

P

E

H

P

P

G

G

W

W

V

V

E

E

Q

H

D

N

A

A

E

N

D

E

I

I

W

W

R

G

T

S

Q

V

A

L

E

A

T

V

I

I

R

A

L

S

A

V

L

I

S

S

Q

E

R

S

Q

G

L

I

Q

S

A

A

S

S

D

Q

L

I

I

A

A

G

V

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

V

T

V

V

V

V

W

W

D

D

R

K

L

D

T

T

G

G

K

S

P

P

I

V

H

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

D

S

R

R

R

Q

T

T

A

S

E

G

E

I

C

C

R

E

R

E

L

L

Q

R

E

E

A

K

G

G

L

Y

E

N

P

D

M

K

I

F

R

R

A

E

K

K

T

T

G

G

L

L

L

L

I

I

D

D

P

P

Y

Y

F

F

S

S

A

G

T

T

K

K

I

V

R

K

W

W

I

I

L

L

D

D

H

N

V

V

E

E

G

G

A

A

R

R

E

E

K

K

A

A

G

E

R

K

G

G

E

E

L

L

A

L

C

F

G

G

T

T

V

I

D

D

S

T

W

W

L

L

V

I

W

W

N

K

L

M

T

S

G

G

G

G

A

K

L

A

H

H

I

V

T

T

D

D

V

Y

S

S

N

N

A

A

A

S

R

R

T

T

L

L

L

M

Y

F

N

N

I

I

E

Y

S

D

R

L

Q

K

W

W

D

D

E

D

E

E

L

L

L

L

N

D

L

I

F

L

E

G

I

V

P

P

S

K

S

S

L

M

L

L

P

P

E

E

V

V

K

K

S

P

T

S

A

S

E

H

I

V

Y

Y

G

A

T

E

T

T

A

V

-

D

H

Y

P
x
H

G

F

I
x
F

G

G

S

K

G

N

I

I

P

P

I

I

A

A

A

G

V

A

A

A

G

G

D

D

Q

Q

Q

Q

A

S

A

A

L

L

F

F

G

G

Q

Q

M

A

C

C

L

F

D

E

P

E

G

G

M

M

A

G

K

K

C

N

T

T

Y

Y

G

G

T

T

G

G

S

C

F

F

L

M

M

L

M

M

N

N

T

T

G

G

P

E

E

K

R

A

R

I

A

K

S

S

K

E

H

H

R

G

L

L

L

L

T

T

T

T

I

I

A

A

W

W

Q

G

I

I

G

D

D

G

E

K

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

T

V

G

A

G

G

S

S

V

A

I

I

Q

Q

W

W

L

L

R

R

D

D

G

G

L

L

R

R

L

M

F

F

D

Q

R

D

T

S

A

S

D

L

C

S

E

E

A

S

L

Y

A

A

G

E

S

K

V

V

P

D

D

S

S

T

G

D

G

G

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

V

G

G

L

L

G

G

A

T

P

P

H

Y

W

W

D

D

P

S

R

D

A

V

R

R

G

G

S

S

L

V

F

F

G

G

I

L

T

T

G

R

G

G

T

T

T

T

G

K

A

E

H

H

I

F

A

I

R

R

A

A

A

T

I

L

E

E

S

S

I

L

C

A

F

Y

Q

Q

V

T

N

K

D

D

L

V

L

L

N

D

A

A

L

M

S

E

E

A

D

D

G

S

G

N

R

I

P

S

V

L

P

K

Q

T

L

L

R

R

I

V

D

D

G

G

G

G

A

A

A

V

T

K

N

N

N

N

L

F

L

L

A

M

Q

Q

F

F

Q

Q

A

G

D

D

I

L

S

L

S

N

L

V

E

P

V

V

V

E

R

R

P

P

Q

E

T

I

L

N

E

E

S

T

T

T

A

A

L

L

G

G

A

A

A

A

C

Y

L

L

A

A

G

G

I

I

A

A

V

V

G

G

L

F

W

W

-

K

N

D

L

R

E

S

E

E

L

I

R

A

K

N

Q

Q

W

W

A

N

I

L

D

D

R

K

T

R

F

F

H

E

G

P

A

E

M

L

P

E

P

E

E

E

E

K

I

R

D

N

R

E

R

L

T

Y

R

K

R

G

W

W

L

Q

K

K

A

A

V

V

E

K

R

A

A

A

H230 (≠ P224) mutation to R: Increased activity.
F232 (≠ I226) mutation to S: Increased activity.

3ge1A 2.7 angstrom crystal structure of glycerol kinase (glpk) from staphylococcus aureus in complex with adp and glycerol
51% identity, 99% coverage: 1:483/490 of query aligns to 7:491/499 of 3ge1A

query
sites
3ge1A

M

L

A

S

I

I

D

D

Q

Q

G

G

T

T

S

T

S

S

S

S

R

R

C

A

I

I

I

L

Y

F

D

N

R

Q

R

K

A

G

N

E

P

I

V

A

A

G

M

V

A

A

Q

Q

Q

R

P

E

L

F

K

K

S

Q

C

Y

Y

F

P

P

E

Q

P

S

G

G

W

W

V

V

E

E

Q

H

D

D

A

A

E

N

D

E

I

I

W

W

R

T

T

S

Q

V

A

L

E

A

T

V

I

M

R

T

L

E

A

V

L

I

S

N

Q

E

R

N

Q

D

L

V

Q

R

A

A

S

D

D

Q

L

I

I

A

A

G

V

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

V

T

V

V

V

V

W

W

D

D

R

K

L

H

T

T

G

G

K

R

P

P

I

I

H

Y

N

H

A

A

I

I

V

V

W

W

Q

Q

D

S

R

R

R

Q

T

T

A

Q

E

S

E

I

C

C

R

S

R

E

L

L

Q

K

E

Q

A

Q

G

G

L

Y

E

E

P

Q

M

T

I

F

R

R

A

D

K

K

T

T

G

G

L

L

L

L

I

L

D

D

P

P

Y

Y

F

F

S

A

A

G

T

T

K

K

I

V

R

K

W

W

I

I

L

L

D

D

H

N

V

V

E

E

G

G

A

A

R

R

E

E

K

K

A

A

G

E

R

N

G

G

E

D

L

L

A

L

C

F

G

G

T

T

V

I

D

D

S

T

W

W

L

L

V

V

W

W

N

K

L

L

T

S

G

G

G

K

A

A

L

A

H

H

I

I

T

T

D

D

V

Y

S

S

N

N

A

A

A

S

R

R

T

T

L

L

L

M

Y

F

N

N

I

I

E

H

S

D

R

L

Q

E

W

W

D

D

E

D

E

E

L

L

L

L

N

E

L

L

F

L

E

T

I

V

P

P

S

K

S

N

L

M

L

L

P

P

E

E

V

V

K

K

S

A

T

S

A

S

E

E

I

V

Y

Y

G

G

T

K

T

T

A

I

-

D

H

Y

P

H

G

F

I

Y

G

G

S

Q

G

E

I

V

P

P

I

I

A

A

A

G

V

V

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

A

C

C

L

F

D

E

P

R

G

G

M

D

A

V

K

K

C

N

T

T

Y

Y

G
|
G

T
|
T

G

G

S

G

F

F

L

M

M

L

M

M

N

N

T

T

G

G

P

D

E

K

R

A

R

V

A

K

S

S

K

E

H

S

R

G

L

L

L

L

T

T

T

T

I

I

A

A

W

Y

Q

G

I

I

G

D

D

G

E

K

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

T

V

G

S

G
|
G

S

S

V

A

I
|
I

Q
|
Q

W

W

L

L

R

R

D

D

G

G

L

L

R

R

L

M

F

I

D

N

R

S

T
x
A

A
x
P

D

Q

C

S

E

E

A

S

L

Y

A

A

G

T

S

R

V

V

P

D

D

S

S

T

G

E

G

G

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

V

G

G

L

L

G

G

A

T

P

P

H

Y

W

W

D

D

P

S

R

E

A

A

R

R

G

G

S

A

L

I

F

F

G

G

I

L

T

T

G

R

G

G

T

T

T

E

G

K

A

E

H

H

I

F

A

I

R

R

A

A

A

T

I

L

E

E

S

S

I

L

C

C

F

Y

Q

Q

V

T

N

R

D

D

L

V

L

M

N

E

A

A

L

M

S

S

E

K

D

D

G

S

G

G

R

I

P

D

V

V

P

Q

Q

S

L

L

R

R

I

V

D

D

G

G

G
|
G

A
|
A

A

V

T

K

N
|
N

N

N

L

F

L

I

A

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

S

V

S

N

L

T

E

S

V

V

V

E

R

R

P

P

Q

E

T

I

L

Q

E

E

S

T

T

T

A

A

L

L

G

G

A

A

A

A

C

F

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

E

N

S

L

K

E

D

E

D

L

I

R

A

K

K

Q

N

W

W

A

K

I

L

D

E

R

E

T

K

F

F

H

D

G

P

A

K

M

M

P

D

P

E

E

G

E

E

I

R

D

E

R

K

R

L

T

Y

R

R

R

G

W

W

L

K

K

K

A

A

V

V

E

E

binding adenosine-5'-diphosphate: G266 (= G259), T267 (= T260), G310 (= G303), I313 (= I306), Q314 (= Q307), A326 (≠ T319), P327 (≠ A320), G411 (= G404), A412 (= A405), N415 (= N408)

Q5HGD2 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Staphylococcus aureus (strain COL)
51% identity, 99% coverage: 1:483/490 of query aligns to 6:490/498 of Q5HGD2

query
sites
Q5HGD2

M

L

A

S

I

I

D

D

Q

Q

G

G

T
|
T

S

T

S

S

S

S

R
|
R

C

A

I

I

I

L

Y

F

D

N

R

Q

R

K

A

G

N

E

P

I

V

A

A

G

M

V

A

A

Q

Q

Q

R

P

E

L

F

K

K

S

Q

C

Y

Y

F

P

P

E

Q

P

S

G

G

W

W

V

V

E

E

Q

H

D

D

A

A

E

N

D

E

I

I

W

W

R

T

T

S

Q

V

A

L

E

A

T

V

I

M

R

T

L

E

A

V

L

I

S

N

Q

E

R

N

Q

D

L

V

Q

R

A

A

S

D

D

Q

L

I

I

A

A

G

V

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

V

T

V

V

V

V

W

W

D

D

R

K

L

H

T

T

G

G

K

R

P

P

I

I

H

Y

N

H

A

A

I

I

V

V

W

W

Q

Q

D

S

R

R

R

Q

T

T

A

Q

E

S

E

I

C

C

R

S

R

E

L

L

Q

K

E

Q

A

Q

G

G

L

Y

E

E

P

Q

M

T

I

F

R

R

A

D

K

K

T

T

G

G

L

L

L

L

I

L

D

D

P

P

Y
|
Y

F

F

S

A

A

G

T

T

K

K

I

V

R

K

W

W

I

I

L

L

D

D

H

N

V

V

E

E

G

G

A

A

R

R

E

E

K

K

A

A

G

E

R

N

G

G

E

D

L

L

A

L

C

F

G

G

T

T

V

I

D

D

S

T

W

W

L

L

V

V

W

W

N

K

L

L

T

S

G

G

G

K

A

A

L

A

H

H

I

I

T

T

D

D

V

Y

S

S

N

N

A

A

A

S

R

R

T

T

L

L

L

M

Y

F

N

N

I

I

E

H

S

D

R

L

Q

E

W

W

D

D

E

D

E

E

L

L

L

L

N

E

L

L

F

L

E

T

I

V

P

P

S

K

S

N

L

M

L

L

P

P

E

E

V

V

K

K

S

A

T

S

A

S

E

E

I

V

Y

Y

G

G

T

K

T

T

A

I

-

D

H

Y

P

H

G

F

I

Y

G

G

S

Q

G

E

I

V

P

P

I

I

A

A

A

G

V

V

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

A

C

C

L

F

D

E

P

R

G

G

M

D

A

V

K

K

C

N

T

T

Y

Y

G

G

T
|
T

G

G

S

G

F

F

L

M

M

L

M

M

N

N

T

T

G

G

P

D

E

K

R

A

R

V

A

K

S

S

K

E

H

S

R

G

L

L

L

L

T

T

T

T

I

I

A

A

W

Y

Q

G

I

I

G

D

D

G

E

K

V

V

A

N

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

T

V

G

S

G
|
G

S

S

V

A

I

I

Q
|
Q

W

W

L

L

R

R

D

D

G

G

L

L

R

R

L

M

F

I

D

N

R

S

T

A

A

P

D

Q

C

S

E

E

A

S

L

Y

A

A

G

T

S

R

V

V

P

D

D

S

S

T

G

E

G

G

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

V

G

G

L

L

G

G

A

T

P

P

H

Y

W

W

D

D

P

S

R

E

A

A

R

R

G

G

S

A

L

I

F

F

G

G

I

L

T

T

G

R

G

G

T

T

T

E

G

K

A

E

H

H

I

F

A

I

R

R

A

A

A

T

I

L

E

E

S

S

I

L

C

C

F

Y

Q

Q

V

T

N

R

D

D

L

V

L

M

N

E

A

A

L

M

S

S

E

K

D

D

G

S

G

G

R

I

P

D

V

V

P

Q

Q

S

L

L

R

R

I

V

D

D

G

G

G
|
G

A

A

A

V

T

K

N
|
N

N

N

L

F

L

I

A

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

S

V

S

N

L

T

E

S

V

V

V

E

R

R

P

P

Q

E

T

I

L

Q

E

E

S

T

T

T

A

A

L

L

G

G

A

A

A

A

C

F

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

E

N

S

L

K

E

D

E

D

L

I

R

A

K

K

Q

N

W

W

A

K

I

L

D

E

R

E

T

K

F

F

H

D

G

P

A

K

M

M

P

D

P

E

E

G

E

E

I

R

D

E

R

K

R

L

T

Y

R

R

R

G

W

W

L

K

K

K

A

A

V

V

E

E

T12 (= T7) binding ADP
R16 (= R11) binding ADP
R82 (= R77) binding glycerol
E83 (= E78) binding glycerol
Y134 (= Y129) binding glycerol
D244 (= D238) binding glycerol
Q245 (= Q239) binding glycerol
T266 (= T260) binding ADP
G309 (= G303) binding ADP
Q313 (= Q307) binding ADP
G410 (= G404) binding ADP
N414 (= N408) binding ADP

O34153 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Enterococcus casseliflavus (Enterococcus flavescens) (see 3 papers)
52% identity, 98% coverage: 1:482/490 of query aligns to 8:491/506 of O34153

query
sites
O34153

M

M

A

A

I

I

D

D

Q

Q

G

G

T

T

S

T

S

S

S

S

R

R

C

A

I

I

I

I

Y

F

D

D

R

R

R

N

A

G

N

K

P

K

V

I

A

G

M

S

A

S

Q

Q

Q

K

P

E

L

F

K

P

S

Q

C

Y

Y

F

P

P

E

K

P

S

G

G

W

W

V

V

E

E

Q

H

D

N

A

A

E

N

D

E

I

I

W

W

R

N

T

S

Q

V

A

Q

E

S

T

V

I

I

R

A

L

G

A

A

L

F

S

I

Q

E

R

S

Q

G

L

I

Q

R

A

P

S

E

D

A

L

I

I

A

A

G

V

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

V

T

V

V

V

V

W

W

D

D

R

K

L

T

T

T

G

G

K

Q

P

P

I

I

H

A

N

N

A

A

I

I

V

V

W

W

Q

Q

D

S

R

R

R

Q

T

S

A

S

E

P

E

I

C

A

R

D

R

Q

L

L

Q

K

E

V

A

D

G

G

L

H

E

T

P

E

M

M

I

I

R

H

A

E

K

K

T

T

G

G

L

L

L

V

I

I

D

D

P

A

Y
|
Y

F

F

S

S

A

A

T

T

K

K

I

V

R

R

W

W

I

L

L

L

D

D

H

N

V

I

E

E

G

G

A

A

R

Q

E

E

K

K

A

A

G

D

R

N

G

G

E

E

L

L

A

L

C

F

G

G

T

T

V

I

D

D

S

S

W

W

L

L

V

V

W

W

N

K

L

L

T

T

G

D

G

G

A

Q

L

V

H

H

I

V

T

T

D

D

V

Y

S

S

N

N

A

A

A

S

R

R

T

T

L

M

L

L

Y

Y

N

N

I

I

E

H

S

K

R

L

Q

E

W

W

D

D

E

Q

E

E

L

I

L

L

N

D

L

L

F

L

E

N

I

I

P

P

S

S

S

S

L

M

L

L

P

P

E

E

V

V

K

K

S

S

T

N

A

S

E

E

I

V

Y

Y

G

G

T

H

T

T

-

R

A

S

H

Y

P
x
H

G

F

I

Y

G

G

S

S

G

E

I

V

P

P

I

I

A

A

A

G

V

M

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

M

C

A

L

F

D

E

P

K

G

G

M

M

A

I

K

K

C

N

T

T

Y

Y

G

G

T

T

G

G

S

A

F

F

L

I

M

V

M

M

N

N

T

T

G

G

P

E

E

E

R

P

R

Q

A

L

S

S

K

D

H

N

R

D

L

L

L

L

T

T

T

T

I

I

A

G

W

Y

Q

G

I

I

G

N

D

G

E

K

V

V

A

Y

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

T

V

G

A

G

G

S

S

V

A

I

I

Q

Q

W

W

L

L

R

R

D

D

G

G

L

L

R

R

L

M

F

I

D

E

R

T

T

S

A

P

D

Q

C

S

E

E

A

E

L

L

A

A

G

A

S

K

V

A

P

K

D

G

S

D

G

N

G

E

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

T

G

G

L

L

G

G

A

A

P

P

H

Y

W

W

D

D

P

S

R

E

A

A

R

R

G

G

S

A

L

V

F

F

G

G

I

L

T

T

G

R

G

G

T

T

T

T

G

K

A

E

H

D

I

F

A

V

R

R

A

A

A

T

I

L

E

Q

S

A

I

V

C

A

F

Y

Q

Q

V

S

N

K

D

D

L

V

L

I

N

D

A

T

L

M

S

K

E

K

D

D

G

S

G

G

R

I

P

D

V

I

P

P

Q

L

L

L

R

K

I

V

D

D

G

G

G

G

A

A

A

A

T

K

N

N

N

D

L

L

L

L

A

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

S

L

S

D

L

I

E

D

V

V

V

Q

R

R

P

A

Q

A

T

N

L

L

E

E

S

T

T

T

A

A

L

L

G

G

A

A

A

A

C

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

K

N

D

L

L

E

D

E

E

L

L

R

K

K

S

Q

M

W

A

A

E

I

E

D

G

R

Q

T

M

F

F

H

T

G

P

A

E

M

M

P

P

P

A

E

E

E

E

I

R

D

D

R

N

R

L

T

Y

R

E

R

G

W

W

L

K

K

Q

A

A

V

V

R84 (= R77) binding glycerol
E85 (= E78) binding glycerol
Y136 (= Y129) binding glycerol
H232 (≠ P224) modified: Phosphohistidine; by HPr; mutation to A: Loss of phosphorylation, no effect on activity.; mutation to E: Loss of phosphorylation, 2.5-fold reduced activity.; mutation to R: Loss of phosphorylation, 3.4-fold increased activity.
D246 (= D238) binding glycerol
Q247 (= Q239) binding glycerol

O34154 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Enterococcus faecalis (strain ATCC 700802 / V583) (see 2 papers)
52% identity, 98% coverage: 1:482/490 of query aligns to 8:490/501 of O34154

query
sites
O34154

M

M

A

A

I

I

D

D

Q

Q

G

G

T

T

S

T

S

S

S

S

R

R

C

A

I

I

I

I

Y

F

D

D

R

K

R

K

A

G

N

N

P

K

V

I

A

G

M

S

A

S

Q

Q

Q

K

P

E

L

F

K

T

S

Q

C

Y

Y

F

P

P

E

N

P

A

G

G

W

W

V

V

E

E

Q

H

D

N

A

A

E

N

D

E

I

I

W

W

R

N

T

S

Q

V

A

Q

E

S

T

V

I

I

R

A

L

G

A

S

L

L

S

I

Q

E

R

S

Q

G

L

V

Q

K

A

P

S

T

D

D

L

I

I

A

A

G

V

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

V

T

V

V

V

V

W

W

D

D

R

K

L

A

T

T

G

G

K

L

P

P

I

I

H

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

D

S

R

R

R

Q

T

T

A

T

E

P

E

I

C

A

R

D

R

Q

L

L

Q

K

E

E

A

D

G

G

L

Y

E

S

P

E

M

M

I

I

R

H

A

E

K

K

T

T

G

G

L

L

L

I

I

I

D

D

P

A

Y

Y

F

F

S

S

A

A

T

T

K

K

I

V

R

R

W

W

I

I

L

L

D

D

H

H

V

V

E

E

G

G

A

A

R

Q

E

E

K

R

A

A

G

E

R

N

G

G

E

E

L

L

A

M

C

F

G

G

T

T

V

I

D

D

S

T

W

W

L

L

V

V

W

W

N

K

L

L

T

T

G

G

G

D

A

T

L

-

H

H

I

V

T

T

D

D

V

Y

S

S

N

N

A

A

A

S

R

R

T

T

L

M

L

L

Y

F

N

N

I

I

E

H

S

D

R

L

Q

D

W

W

D

D

E

Q

E

E

L

I

L

L

N

D

L

L

F

L

E

N

I

I

P

P

S

R

S

V

L

M

L

L

P

P

E

K

V

V

K

V

S

S

T

N

A

S

E

E

I

V

Y

Y

G

G

-

L

T

T

T

K

A

N

H

Y

P
x
H

G

F

I

Y

G

G

S

S

G

E

I

V

P

P

I

I

A

A

A

G

V

M

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

M

C

A

L

F

D

E

P

P

G

G

M

M

A

V

K

K

C

N

T

T

Y

Y

G

G

T

T

G

G

S

S

F

F

L

I

M

V

M

M

N

N

T

T

G

G

P

E

E

E

R

P

R

Q

A

L

S

S

K

K

H

N

R

N

L

L

L

L

T

T

T

T

I

I

A

G

W

Y

Q

G

I

I

G

N

D

G

E

K

V

V

A

Y

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

T

V

G

A

G

G

S

S

V

A

I

I

Q

Q

W

W

L

L

R

R

D

D

G

G

L

L

R

K

L

M

F

L

D

Q

R

T

T

A

A

A

D

E

C

S

E

E

A

A

L

V

A

A

G

K

S

A

V

S

P

T

D

G

S

H

G

N

G

E

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

T

G

G

L

L

G

G

A

A

P

P

H

Y

W

W

D

D

P

S

R

Q

A

A

R

R

G

G

S

A

L

V

F

F

G

G

I

L

T

T

G

R

G

G

T

T

T

T

G

R

A

E

H

D

I

F

A

V

R

K

A

A

A

T

I

L

E

Q

S

A

I

V

C

A

F

Y

Q

Q

V

V

N

R

D

D

L

I

L

I

N

D

A

T

L

M

S

K

E

E

D

D

G

T

G

G

R

I

P

D

V

I

P

P

Q

V

L

L

R

K

I

V

D

D

G

G

G

G

A

A

A

A

T

N

N

N

N

D

L

F

L

L

A

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

S

L

S

N

L

T

E

A

V

V

V

Q

R

R

P

A

Q

H

T

N

L

L

E

E

S

T

T

T

A

A

L

L

G

G

A

A

A

A

C

F

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

K

N

D

L

L

E

E

E

E

L

I

R

K

K

A

Q

F

W

Q

A

E

I

E

D

G

R

Q

T

Q

F

F

H

E

G

P

A

I

M

M

P

A

P

E

E

E

E

E

I

R

D

E

R

D

R

L

T

Y

R

E

R

G

W

W

L

Q

K

Q

A

A

V

V

H231 (≠ P224) modified: Phosphohistidine; by HPr

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3h3nX Glycerol kinase h232r with glycerol (see paper)
52% identity, 98% coverage: 1:482/490 of query aligns to 7:490/501 of 3h3nX

query
sites
3h3nX

M

M

A

A

I

I

D

D

Q

Q

G

G

T
|
T

S

T

S

S

S

S

R

R

C

A

I

I

I

I

Y

F

D

D

R

R

R

N

A

G

N

K

P

K

V

I

A

G

M

S

A

S

Q

Q

Q

K

P

E

L

F

K

P

S

Q

C

Y

Y

F

P

P

E

K

P

S

G

G

W

W

V

V

E

E

Q

H

D

N

A

A

E

N

D

E

I

I

W

W

R

N

T

S

Q

V

A

Q

E

S

T

V

I

I

R

A

L

G

A

A

L

F

S

I

Q

E

R

S

Q

G

L

I

Q

R

A

P

S

E

D

A

L

I

I

A

A

G

V

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

V

T

V

V

V

V

W

W

D

D

R

K

L

T

T

T

G

G

K

Q

P

P

I

I

H

A

N

N

A

A

I

I

V

V

W
|
W

Q

Q

D

S

R

R

R

Q

T

S

A

S

E

P

E

I

C

A

R

D

R

Q

L

L

Q

K

E

V

A

D

G

G

L

H

E

T

P

E

M

M

I

I

R

H

A

E

K

K

T

T

G

G

L

L

L

V

I

I

D

D

P

A

Y
|
Y

F

F

S

S

A

A

T

T

K

K

I

V

R

R

W

W

I

L

L

L

D

D

H

N

V

I

E

E

G

G

A

A

R

Q

E

E

K

K

A

A

G

D

R

N

G

G

E

E

L

L

A

L

C

F

G

G

T

T

V

I

D

D

S

S

W

W

L

L

V

V

W

W

N

K

L

L

T

T

G

D

G

G

A

Q

L

V

H

H

I

V

T

T

D

D

V

Y

S

S

N

N

A

A

A

S

R

R

T

T

L

M

L

L

Y

Y

N

N

I

I

E

H

S

K

R

L

Q

E

W

W

D

D

E

Q

E

E

L

I

L

L

N

D

L

L

F

L

E

N

I

I

P

P

S

S

S

S

L

M

L

L

P

P

E

E

V

V

K

K

S

S

T

N

A

S

E

E

I

V

Y

Y

G

G

T

H

T

T

-

R

A

S

H

Y

P

R

G

F

I

Y

G

G

S

S

G

E

I

V

P

P

I

I

A

A

A

G

V

M

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

M

C

A

L

F

D

E

P

K

G

G

M

M

A

I

K

K

C

N

T

T

Y

Y

G

G

T
|
T

G

G

S

A

F
|
F

L

I

M

V

M

M

N

N

T

T

G

G

P

E

E

E

R

P

R

Q

A

L

S

S

K

D

H

N

R

D

L

L

L

L

T

T

T

T

I

I

A

G

W

Y

Q

G

I

I

G

N

D

G

E

K

V

V

A

Y

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

T

V

G

A

G

G

S

S

V

A

I

I

Q

Q

W

W

L

L

R

R

D

D

G

G

L

L

R

R

L

M

F

I

D

E

R

T

T

S

A

P

D

Q

C

S

E

E

A

E

L

L

A

A

G

A

S

K

V

A

P

K

D

G

S

D

G

N

G

E

V

V

V

Y

F

V

V

V

P

P

A

A

L

F

S

T

G

G

L

L

G

G

A

A

P

P

H

Y

W

W

D

D

P

S

R

E

A

A

R

R

G

G

S

A

L

V

F

F

G

G

I

L

T

T

G

R

G

G

T

T

T

T

G

K

A

E

H

D

I

F

A

V

R

R

A

A

A

T

I

L

E

Q

S

A

I

V

C

A

F

Y

Q

Q

V

S

N

K

D

D

L

V

L

I

N

D

A

T

L

M

S

K

E

K

D

D

G

S

G

G

R

I

P

D

V

I

P

P

Q

L

L

L

R

K

I

V

D

D

G

G

G

G

A

A

A

A

T

K

N

N

N

D

L

L

L

L

A

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

S

L

S

D

L

I

E

D

V

V

V

Q

R

R

P

A

Q

A

T

N

L

L

E

E

S

T

T

T

A

A

L

L

G

G

A

A

A

A

C

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

L

F

W

W

-

K

N

D

L

L

E

D

E

E

L

L

R

K

K

S

Q

M

W

A

A

E

I

E

D

G

R

Q

T

M

F

F

H

T

G

P

A

E

M

M

P

P

P

A

E

E

E

E

I

R

D

D

R

N

R

L

T

Y

R

E

R

G

W

W

L

K

K

Q

A

A

V

V

binding glycerol: R83 (= R77), E84 (= E78), W103 (= W97), Y135 (= Y129), D245 (= D238), F270 (= F263)
binding phosphate ion: T13 (= T7), T267 (= T260)

O86033 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
50% identity, 99% coverage: 1:484/490 of query aligns to 6:492/497 of O86033

query
sites
O86033

M

L

A

A

I

I

D

D

Q

Q

G

G

T

T

S

T

S

S

S

T

R

R

C

A

I

I

I

V

Y

F

D

D

R

G

R

N

A

Q

N

K

P

I

V

A

A

G

M

V

A

G

Q

Q

Q

K

P

E

L

F

K

K

S

Q

C

H

Y

F

P

P

E

K

P

S

G

G

W

W

V

V

E

E

Q

H

D

D

A

P

E

E

D

E

I

I

W

W

R

Q

T

T

Q

V

A

V

E

S

T

T

I

V

R

K

L

E

A

A

L

I

S

E

Q

K

R

S

Q

G

L

I

Q

T

A

A

S

N

D

D

L

I

I

A

A

A

V

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

V

V

V

V

V

V

W

W

D

D

R

R

L

E

T

T

G

G

K

K

P

P

I

I

H

H

N

N

A

A

I

I

V

V

W

W

Q

Q

D

D

R

R

R

R

T

T

A

A

E

A

E

F

C

C

R

D

R

K

L

L

Q

K

E

K

A

K

G

G

L

L

E

E

P

K

M

T

I

F

R

V

A

K

K

K

T

T

G

G

L

L

L

L

I

L

D

D

P

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

L

R

N

W

W

I

L

L

L

D

S

H

N

V

V

E

K

G

G

A

A

R

Q

E

V

K

R

A

A

G

A

R

K

G

G

E

E

L

L

A

C

C

F

G

G

T

T

V

I

D

D

S

T

W

F

L

L

V

I

W

W

N

R

L

L

T

T

G

G

G

G

A

E

L

C

H

F

I

C

T

T

D

D

V

A

S

T

N

N

A

A

A

S

R

R

T

T

L

L

L

L

Y

Y

N

N

I

I

E

A

S

E

R

N

Q

A

W

W

D

D

E

D

E

E

L

L

L

T

N

E

L

V

F

L

E

R

I

V

P

P

S

K

S

E

L

M

L

L

P

P

E

E

V

V

K

K

S

D

T

C

A

A

E

A

I

D

Y

F

G

G

T

V

T

T

A

D

H

P

P

S

G

L

I

F

G

G

S

A

G

A

I

I

P

P

I

I

A

L

A

G

V

V

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

T

F

I

G

G

Q

Q

M

A

C

C

L

F

D

K

P

P

G

G

M

M

A

L

K

K

C

S

T

T

Y

Y

G

G

T

T

G

G

S

C

F

F

L

A

M

L

M

L

N

N

T

T

G

G

P

K

E

D

R

M

R

V

A

R

S

S

K

K

H

N

R

R

L

L

L

L

T

T

T

T

I

I

A

A

W

Y

Q

R

I

L

G

D

D

G

E

E

V

T

A

T

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

T

V

G

A

G

G

S

A

V

A

I

V

Q

Q

W

W

L

L

R

R

D

D

G

G

L

L

R

K

L

V

F

I

D

K

R

A

T

A

A

P

D

D

C

T

E

G

A

S

L

L

A

A

G

E

S

S

V

A

P

D

D

P

S

S

G

Q

G

E

V

V

V

Y

F

L

V

V

P

P

A

A

L

F

S

T

G

G

L

L

G

G

A

A

P

P

H

H

W

W

D

D

P

P

R

D

A

A

R

R

G

G

S

A

L

I

F

F

G

G

I

M

T

T

G

R

G

N

T

T

T

G

G

P

A

A

H

E

I

F

A

A

R

R

A

A

A

A

I

L

E

E

S

A

I

V

C

C

F

Y

Q

Q

V

T

N

R

D

D

L

L

L

L

N

E

A

A

L

M

S

H

E

K

D

D

G

W

G

R

R

R

P

N

V

G

P

N

Q

D

-

T

-

V

L

L

R

R

I

V

D

D

G

G

G

G

A

M

A

V

T

A

N

S

N

D

L

W

L

T

A

M

Q

Q

F

R

Q

L

A

S

D

D

I

L

S

L

S

D

L

A

E

P

V

V

V

D

R

R

P

P

Q

V

T

I

L

L

E

E

S

T

T

T

A

A

L

L

G

G

A

V

A

A

C

W

L

L

A

A

G

G

I

S

A

R

V

A

G

G

L

V

W

W

-

P

N

N

L

Q

E

E

E

A

L

F

R

A

K

K

Q

S

W

W

A

A

I

R

D

D

R

R

T

R

F

F

H

E

G

P

A

H

M

M

P

D

P

E

E

A

E

T

I

R

D

K

R

V

R

K

T

L

R

K

R

G

W

W

L

R

K

S

A

A

V

V

E

K

R

R

R82 (= R77) binding glycerol
E83 (= E78) binding glycerol
Y134 (= Y129) binding glycerol
D243 (= D238) binding glycerol
Q244 (= Q239) binding glycerol

6k76A Glycerol kinase form thermococcus kodakarensis, complex structure with substrate.
48% identity, 100% coverage: 1:489/490 of query aligns to 2:481/485 of 6k76A

query
sites
6k76A

M

L

A

S

I

L

D

D

Q

E

G

G

T
|
T

S

T

S

S

S

A

R

R

C

A

I

I

I

I

Y

F

D

D

R

R

R

E

A

S

N

N

P

I

V

H

A

G

M

I

A

G

Q

Q

Q

Y

P

E

L

F

K

P

S

Q

C

H

Y

Y

P

P

E

R

P

P

G

G

W

W

V

V

E

E

Q

H

D

N

A

P

E

E

D

E

I

I

W

W

R

D

T

A

Q

Q

A

L

E

R

T

A

I

I

R

K

L

D

A

A

L

I

S

-

Q

Q

R

S

Q

A

L

R

Q

E

A

P

S

N

D

Q

L

I

I

A

A

A

V

I

G

G

I

V

T

T

N

N

Q

Q

R

R

E

E

T

T

V

T

V

L

V

V

W

W

D

D

R

K

L

-

T

D

G

G

K

K

P

P

I

L

H

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

D

C

R

R

R

R

T

T

A

A

E

E

E

M

C

V

R

E

R

E

L

I

Q

K

-

R

E

E

A

Y

G

G

L

-

E

-

P

T

M

M

I

I

R

K

A

E

K

K

T

T

G

G

L

L

L

V

I

P

D

D

P

A

Y

Y

F

F

S

S

A

A

T

S

K

K

I

L

R

K

W

W

I

L

L

L

D

D

H

N

V

V

E

P

G

G

A

L

R

R

E

E

K

K

A

A

G

E

R

K

G

G

E

E

L

V

A

M

C

F

G

G

T

T

V

V

D

D

S

T

W

F

L

L

V

I

W

Y

N

R

L

L

T

T

G

G

G

E

A

-

L

-

H

H

I

V

T

T

D

D

V

Y

S

S

N

N

A

A

A

S

R

R

T

T

L

M

L

L

Y

F

N

N

I

I

E

K

S

K

R

L

Q

D

W

W

D

D

E

D

E

E

L

L

L

L

N

E

L

L

F

F

E

D

I

I

P

P

S

E

S

S

L

V

L

L

P

P

E

E

V

V

K

R

S

E

T

S

A

S

E

E

I

V

Y

Y

G

G

T

Y

T

T

A

K

H

K

P

E

G

L

I

-

G

-

S

A

G

E

I

I

P

P

I

V

A

S

A

G

V

D

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

M

A

C

A

L

F

D

E

P

A

G

G

M

M

A

V

K

K

C

A

T

T

Y

Y

G

G

T
|
T

G

G

S

S

F

F

L

I

M

L

M

V

N

N

T

T

G

D

P

K

E

M

R

V

R

L

A

Y

S

S

K

D

H

N

R

-

L

L

L

L

T

T

T

T

I

I

A

A

W

W

Q

G

I

L

G

N

D

G

E

R

V

V

A

S

Y

Y

A

A

L

L

E

E

G

G

S

S

V

I

F

F

T

V

G

T

G
|
G

S

A

V

A

I
x
V

Q
|
Q

W

W

L

L

R

R

D

D

G

G

L

I

R

K

L

I

F

I

D

K

R

H

T

A

A

S

D

E

C

T

E

E

A

E

L

L

A

A

G

T

S

K

V

L

P

E

D

S

S

N

G

E

G

G

V

V

V

Y

F

F

V

V

P

P

A

A

L

F

S

V

G

G

L

L

G

G

A

A

P

P

H

Y

W

W

D

D

P

Q

R

F

A

A

R

R

G

G

S

I

L

I

F

I

G

G

I

I

T

T

G

R

G

G

T

T

T

G

G

R

A

E

H

H

I

L

A

A

R

R

A

A

A

T

I

L

E

E

S

A

I

I

C

A

F

Y

Q

L

V

T

N

R

D

D

L

V

L

V

N

D

A

E

L

M

S

E

E

K

D

-

G

-

G

L

R

V

P

Q

V

I

P

K

Q

E

L

L

R

R

I

V

D

D

G

G

G
|
G

A
|
A

A

T

T

A

N
|
N

N

D

L

F

L

L

A

M

Q

Q

F

F

Q

Q

A

A

D

D

I

I

S

L

S

N

L

R

E

K

V

V

V

I

R

R

P

P

Q

V

T

V

L

K

E

E

S

T

T

T

A

A

L

L

G

G

A

A

A

A

C

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

D

L

Y

W

W

-

A

N

D

L

T

E

R

E

E

L

I

R

A

K

E

Q

L

W

W

A

K

I

A

D

E

R

R

T

I

F

F

H

E

G

P

A

K

M

M

P

D

P

E

E

K

E

T

I

R

D

E

R

R

R

L

T

Y

R

K

R

G

W

W

L

K

K

E

A

A

V

V

E

K

R

R

A

A

K

M

N

G

W

W

A

A

binding phosphoaminophosphonic acid-adenylate ester: T8 (= T7), T254 (= T260), G296 (= G303), V299 (≠ I306), Q300 (= Q307), G395 (= G404), A396 (= A405), N399 (= N408)

5gn6A Crystal structure of glycerol kinase from trypanosoma brucei gambiense complexed with cumarin derivative-17b (see paper)
42% identity, 100% coverage: 2:489/490 of query aligns to 8:512/513 of 5gn6A

query
sites
5gn6A

A

S

I

I

D

D

Q

Q

G

G

T

T

S

T

S

S

S

T

R

R

C

F

I

I

I

I

Y

F

D

D

R

E

R

R

A

Q

N

R

P

P

V

V

A

S

M

V

A

H

Q

Q

Q

V

P

P

L

H

K

T

S

Q

C

H

Y

T

P

P

E

H

P

P

G

G

W

W

V

L

E

E

Q

H

D

D

A

P

E

M

D

E

I

I

W

F

R

R

T

S

Q

A

A

C

E

K

T

C

I

M

R

S

L

V

A

A

L

I

S

A

Q

K

-

L

R

R

Q

Q

L

K

Q

D

A

A

S

S

-

F

-

R

D

K

L

I

I

E

A

A

V

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

V

T

V

V

V

A

W

W

D

D

R

R

L

V

T

T

G

K

K

E

P

P

I

L

H

C

N

Y

A

A

I

P

V

V

W
|
W

Q

N

D

D

R

L

R

R

T

T

A

Y

E

D

E

I

C

T

R

K

R

K

L

V

-

T

-

A

Q

E

E

L

A

G

G

G

L

G

E

D

P

S

M

M

I

F

R

A

A

S

K

K

-

I

T

T

G

G

L

L

L

P

I

V

D

S

P

T

Y
|
Y

F

F

S

A

A

A

T

F

K

K

I

M

R

R

W

W

I

M

L

L

D

E

H

N

V

V

E

P

G

A

A

V

R

A

E

D

K

A

A

C

G

R

R

R

G

G

E

T

L

L

A

C

C

F

G

G

T

T

V

I

D

D

S

T

W

W

L

L

V

M

W

Y

N

K

L

L

T

S

G

G

G

G

A

K

L

A

H

F

I

V

T

T

D

D

V

V

S

T

N

N

A

A

A

S

R
|
R

T

T

L

F

L

L

Y

M

N

D

I

L

E

R

S

T

R

R

Q

K

W

W

D

S

E

P

E

E

L

L

L

C

N

E

L

K

F

L

E

K

I

I

P

P

S

M

S

E

L

T

L

L

P

P

E

E

V

I

K

R

S

S

T

N

A

S

E

E

I

L

Y

F

G

G

T

Y

-

V

-

E

T

T

A

D

H

E

P

C

G

G

I

V

G

A

S

A

G

A

I

L

-

N

-

E

-

R

-

T

P

P

I

I

A

M

A

G

V

S

A

I

G

G

D
|
D

Q

Q

Q

Q

A

S

A

A

L

L

F

F

G

G

Q

N

M

M

C

C

L

F

D

E

P

K

G

G

M

E

A

A

K

K

C

N

T

T

Y

Y

G

G

T

T

G

G

S

C

F
|
F

L

L

M

L

M

M

N

N

T

V

G

G

P

E

E

E

R

A

R

R

A

F

S

S

K

K

H

H

R

G

L

L

L
|
L

T

S

T

T

I

V

A

G

W

F

Q

Q

I

V

G

G

D

R

E

D

-

G

-

P

V

C

A

Y

Y

Y

A

A

L

L

E
|
E

G

G

S
x
A

V

I

F

A

T

C

G

A

G

G

S

A

V

T

I

V

Q

E

W

W

L

M

R

R

D

R

G

N

L

M

R

N

L

L

F

F

D

S

R

H

T

I

A

T

D

E

C

C

E

E

A

K

L

L

A

A

G

R

S

S

V

V

P

P

D

G

S

T

G

Q

G

G

V

I

V

V

F

F

V

V

P

P

A

A

L

F

S

S

G

G

L

L

G

L

A

A

P

P

H

Y

W

W

D

D

P

P

R

S

A

A

R

R

G

G

S

T

L

I

F

V

G

G

I

M

T

T

G

L

G

K

T

T

T

T

G

R

A

A

H

H

I

V

A

I

R

R

A

A

A

A

I

L

E

Q

S

A

I

I

C

A

F

L

Q

Q

V

L

N

N

D

D

L

V

L

V

N

G

A

S

L

M

S

K

E

R

D

D

G

A

G

G

R

L

P

N

V

L

P

S

Q

S

L

L

R

R

I

V

D

D

G

G

G

G

A

L

A

S

T

K

N

N

N

G

L

L

L

L

A

M

Q

E

F

I

Q

Q

A

A

D

S

I

L

S

L

S

G

L

V

E

D

V

I

V

L

R

V

P

P

Q

S

T

M

L

H

E

E

S

T

T

T

A

A

L

L

G

G

A

A

A

A

C

L

L

C

A

A

G

G

I

L

A

A

V

A

G

G

L

V

W

W

-

T

N

S

L

L

E

E

E

E

L

V

R

K

K

A

Q

V

W

S

A

R

I

R

D

E

R

N

T

S

F

W

H

K

G

T

A

V

M

S

P

P

P

S

-

G

-

S

-

A

E

M

E

E

I

R

D

E

R

A

R

M

T

I

R

A

R

E

W

W

L

R

K

E

A

A

V

L

E

K

R

R

A

T

K

K

N

-

W

W

A

A

binding 4-butyl-7,8-bis(oxidanyl)chromen-2-one: Y141 (= Y129), R194 (= R182), F281 (= F263), L298 (= L280), E316 (= E296), A318 (≠ S298)
binding glycerol: R86 (= R77), E87 (= E78), W106 (= W97), D256 (= D238)

Query Sequence

>WP_232422309.1 NCBI__GCF_000372865.1:WP_232422309.1
MAIDQGTSSSRCIIYDRRANPVAMAQQPLKSCYPEPGWVEQDAEDIWRTQAETIRLALSQ
RQLQASDLIAVGITNQRETVVVWDRLTGKPIHNAIVWQDRRTAEECRRLQEAGLEPMIRA
KTGLLIDPYFSATKIRWILDHVEGAREKAGRGELACGTVDSWLVWNLTGGALHITDVSNA
ARTLLYNIESRQWDEELLNLFEIPSSLLPEVKSTAEIYGTTAHPGIGSGIPIAAVAGDQQ
AALFGQMCLDPGMAKCTYGTGSFLMMNTGPERRASKHRLLTTIAWQIGDEVAYALEGSVF
TGGSVIQWLRDGLRLFDRTADCEALAGSVPDSGGVVFVPALSGLGAPHWDPRARGSLFGI
TGGTTGAHIARAAIESICFQVNDLLNALSEDGGRPVPQLRIDGGAATNNLLAQFQADISS
LEVVRPQTLESTALGAACLAGIAVGLWNLEELRKQWAIDRTFHGAMPPEEIDRRTRRWLK
AVERAKNWAD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory