SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_237392702.1 NCBI__GCF_001579945.1:WP_237392702.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1bjoA The structure of phosphoserine aminotransferase from e. Coli in complex with alpha-methyl-l-glutamate (see paper)
58% identity, 99% coverage: 2:358/359 of query aligns to 1:359/360 of 1bjoA

query
sites
1bjoA

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active site: W100 (= W101), D172 (= D171), K196 (= K195)
binding alpha-methyl-l-glutamic acid: S7 (≠ A8), W100 (= W101), T151 (= T150), K196 (= K195)
binding pyridoxal-5'-phosphate: G74 (≠ A75), R75 (≠ T76), W100 (= W101), T151 (= T150), D172 (= D171), S174 (= S173), Q195 (= Q194), K196 (= K195)

3qboB Crystal structure of phosphoserine aminotransferase from yersinia pestis co92
59% identity, 99% coverage: 2:358/359 of query aligns to 1:358/359 of 3qboB

query
sites
3qboB

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H

binding pyridoxal-5'-phosphate: G73 (= G74), A74 (= A75), R75 (≠ T76), W100 (= W101), T151 (= T150), D171 (= D171), S173 (= S173), K195 (= K195), N236 (= N236), T237 (= T237)

7t7jB Crystal structure of phosphoserine aminotransferase from klebsiella pneumoniae subsp. Pneumoniae in complex with pyridoxal phosphate
57% identity, 99% coverage: 2:358/359 of query aligns to 1:359/360 of 7t7jB

query
sites
7t7jB

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A

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H

H

binding pyridoxal-5'-phosphate: G73 (= G74), G74 (≠ A75), R75 (≠ T76), W100 (= W101), T151 (= T150), D172 (= D171), S174 (= S173), Q195 (= Q194), K196 (= K195), N237 (= N236), T238 (= T237)

Q96255 Phosphoserine aminotransferase 1, chloroplastic; AtPSAT1; Phosphohydroxythreonine aminotransferase; EC 2.6.1.52 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
54% identity, 100% coverage: 2:359/359 of query aligns to 72:430/430 of Q96255

query
sites
Q96255

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G

G

V

V

E

E

F

F

H

K

F

D

I

Y

P

P

D

-

T

-

N

-

A

-

E

-

V

V

P

P

-

K

-

N

-

G

-

F

L

L

V

V

A

A

D
|
D

L

M

S

S

S

S

T

N

I

F

L

C

S

S

R

K

P

P

L

V

D

D

V

V

S

S

K

K

F

F

G

G

L

V

I

I

Y

Y

A

G

G

G

A

A

Q
|
Q

K
|
K

N

N

I

V

G

G

P

P

A

S

G

G

L

V

A

T

L

I

V

V

I

I

V

I

R

R

E

K

D

D

L

L

L

I

G

G

R

N

A

A

R

Q

A

D

G

I

T

T

P

P

T

V

I

M

L

L

D

D

F

Y

A

K

A

I

M

H

A

D

K

E

D

N

G

S

S

S

M

L

L

Y

N
|
N

T
|
T

P

P

P

P

T

C

Y

F

A

G

W

I

Y

Y

V

M

A

C

G

G

L

L

V

V

F

F

Q

E

W

D

L

L

R

L

R

E

Q

Q

G

G

G

G

L

L

E

K

A

E

M

V

G

E

K

K

R

K

N

N

Q

Q

A

R

K

K

A

A

A

D

K

L

L

L

Y

Y

E

N

A

A

I

I

D

E

D

E

S

S

S

N

-

G

F

F

Y

F

A

R

N

C

P

P

V

V

A

E

K

K

G

S

A

V

R

R

S

S

W

L

M

M

N

N

V

V

P

P

F

F

T

T

L

L

A

E

R

K

P

S

E

E

L

L

D

E

K

A

T

E

F

F

I

I

A

K

E

E

A

A

R

A

E

K

A

E

G

K

L

M

V

V

T

Q

L

L

E

K

G

G

H

H

R

R

S

S

V

V

G

G

G

G

M

M

R

R

A

A

S

S

L

I

Y

Y

N

N

A

A

M

M

P

P

M

L

E

A

G

G

V

V

D

E

A

K

L

L

V

V

G

A

F

F

M

M

R

K

E

D

F

F

E

Q

R

A

R

K

H

H

A

A

AT 145:146 (= AT 75:76) binding pyridoxal 5'-phosphate
W171 (= W101) binding pyridoxal 5'-phosphate
T221 (= T150) binding pyridoxal 5'-phosphate
D241 (= D171) binding pyridoxal 5'-phosphate
Q264 (= Q194) binding pyridoxal 5'-phosphate
K265 (= K195) modified: N6-(pyridoxal phosphate)lysine
NT 306:307 (= NT 236:237) binding pyridoxal 5'-phosphate

6czzA Crystal structure of arabidopsis thaliana phosphoserine aminotransferase isoform 1 (atpsat1) in complex with plp- phosphoserine geminal diamine intermediate (see paper)
54% identity, 100% coverage: 2:359/359 of query aligns to 2:360/360 of 6czzA

query
sites
6czzA

R

R

V

V

F

F

N

N

F

F

S

A

A

A

G

G

P

P

A

A

V

T

L

L

P

P

L

E

E

N

V

V

L

L

E

L

Q

K

A

A

R

Q

D

A

E

D

M

L

L

Y

D

N

W

W

H

R

G

G

T

S

G

G

M

M

S

S

V

V

M

M

E

E

M

M

S

S

H

H

R

R

G

G

K

K

A

E

F

F

V

L

S

S

I

I

A

I

A

Q

Q

K

A

A

E

E

A

S

D

D

L

L

R

R

E

Q

L

L

L

L

Q

E

V

I

P

P

A

Q

N

E

Y

Y

K

S

V

V

L

L

F

F

L

L

Q

Q

G

G

G
|
G

A
|
A

T
|
T

A

T

Q

Q

F

F

A

A

A

A

I

L

P

P

M

L

N

N

L

L

A

C

G

K

P

S

D

D

S

D

V

T

V

V

D

D

Y

F

V

V

H

V

T

T

G

G

A

S

W
|
W

S

G

K

D

K

K

A

A

I

V

G

K

E

E

A

A

K

K

R

K

Y

Y

A

C

N

K

V

T

N

N

V

V

A

I

-

W

A

S

N

G

A

K

G

S

E

E

P

K

Y

Y

A

T

S

K

I

V

P

P

A

S

Q

F

E

E

S

E

W

L

Q

E

R

Q

S

T

P

P

N

D

A

A

A

K

Y

Y

L

L

H

H

Y

I

T

C

P

A

N

N

E

E

T
|
T

I

I

G

H

G

G

V

V

E

E

F

F

H

K

F

D

I

Y

P

P

D

-

T

-

N

-

A

-

E

-

V

V

P

P

-

K

-

N

-

G

-

F

L

L

V

V

A

A

D
|
D

L

M

S
|
S

S

S

T

N

I

F

L

C

S

S

R

K

P

P

L

V

D

D

V

V

S

S

K

K

F

F

G

G

L

V

I

I

Y

Y

A

G

G

G

A

A

Q
|
Q

K
|
K

N

N

I

V

G

G

P

P

A

S

G

G

L

V

A

T

L

I

V

V

I

I

V

I

R

R

E

K

D

D

L

L

L

I

G

G

R

N

A

A

R

Q

A

D

G

I

T

T

P

P

T

V

I

M

L

L

D

D

F

Y

A

K

A

I

M

H

A

D

K

E

D

N

G

S

S

S

M

L

L

Y

N
|
N

T
|
T

P

P

P

P

T

C

Y

F

A

G

W

I

Y

Y

V

M

A

C

G

G

L

L

V

V

F

F

Q

E

W

D

L

L

R

L

R

E

Q

Q

G

G

G

G

L

L

E

K

A

E

M

V

G

E

K

K

R

K

N

N

Q

Q

A

R

K

K

A

A

A

D

K

L

L

L

Y

Y

E

N

A

A

I

I

D

E

D

E

S

S

S

N

-

G

F

F

Y

F

A

R

N

C

P

P

V

V

A

E

K

K

G

S

A

V

R

R

S

S

W

L

M

M

N

N

V

V

P

P

F

F

T

T

L

L

A

E

R

K

P

S

E

E

L

L

D

E

K

A

T

E

F

F

I

I

A

K

E

E

A

A

R

A

E

K

A

E

G

K

L

M

V

V

T

Q

L

L

E

K

G

G

H
|
H

R
|
R

S

S

V

V

G

G

G

G

M

M

R
|
R

A

A

S

S

L

I

Y

Y

N

N

A

A

M

M

P

P

M

L

E

A

G

G

V

V

D

E

A

K

L

L

V

V

G

A

F

F

M

M

R

K

E

D

F

F

E

Q

R

A

R

K

H

H

A

A

binding pyridoxal-5'-phosphate: G74 (= G74), A75 (= A75), T76 (= T76), W101 (= W101), T151 (= T150), D171 (= D171), S173 (= S173), Q194 (= Q194), K195 (= K195), N236 (= N236), T237 (= T237)
binding phosphoserine: W101 (= W101), T151 (= T150), K195 (= K195), H326 (= H325), R327 (= R326), R333 (= R332)

6czyA Crystal structure of arabidopsis thaliana phosphoserine aminotransferase isoform 1 (atpsat1) in complex with pyridoxamine-5'- phosphate (pmp) (see paper)
54% identity, 100% coverage: 2:359/359 of query aligns to 4:362/362 of 6czyA

query
sites
6czyA

R

R

V

V

F

F

N

N

F

F

S

A

A

A

G

G

P

P

A

A

V

T

L

L

P

P

L

E

E

N

V

V

L

L

E

L

Q

K

A

A

R

Q

D

A

E

D

M

L

L

Y

D

N

W

W

H

R

G

G

T

S

G

G

M

M

S

S

V

V

M

M

E

E

M

M

S

S

H

H

R

R

G

G

K

K

A

E

F

F

V

L

S

S

I

I

A

I

A

Q

Q

K

A

A

E

E

A

S

D

D

L

L

R

R

E

Q

L

L

L

L

Q

E

V

I

P

P

A

Q

N

E

Y

Y

K

S

V

V

L

L

F

F

L

L

Q

Q

G

G

G
|
G

A
|
A

T
|
T

A

T

Q

Q

F

F

A

A

A

A

I

L

P

P

M

L

N

N

L

L

A

C

G

K

P

S

D

D

S

D

V

T

V

V

D

D

Y

F

V

V

H

V

T

T

G

G

A

S

W
|
W

S

G

K

D

K

K

A

A

I

V

G

K

E

E

A

A

K

K

R

K

Y

Y

A

C

N

K

V

T

N

N

V

V

A

I

-

W

A

S

N

G

A

K

G

S

E

E

P

K

Y

Y

A

T

S

K

I

V

P

P

A

S

Q

F

E

E

S

E

W

L

Q

E

R

Q

S

T

P

P

N

D

A

A

A

K

Y

Y

L

L

H

H

Y

I

T

C

P

A

N

N

E

E

T
|
T

I

I

G

H

G

G

V

V

E

E

F

F

H

K

F

D

I

Y

P

P

D

-

T

-

N

-

A

-

E

-

V

V

P

P

-

K

-

N

-

G

-

F

L

L

V

V

A

A

D
|
D

L

M

S

S

S

S

T

N

I

F

L

C

S

S

R

K

P

P

L

V

D

D

V

V

S

S

K

K

F

F

G

G

L

V

I

I

Y

Y

A

G

G

G

A

A

Q
|
Q

K
|
K

N

N

I

V

G

G

P

P

A

S

G

G

L

V

A

T

L

I

V

V

I

I

V

I

R

R

E

K

D

D

L

L

L

I

G

G

R

N

A

A

R

Q

A

D

G

I

T

T

P

P

T

V

I

M

L

L

D

D

F

Y

A

K

A

I

M

H

A

D

K

E

D

N

G

S

S

S

M

L

L

Y

N
|
N

T
|
T

P

P

P

P

T

C

Y

F

A

G

W

I

Y

Y

V

M

A

C

G

G

L

L

V

V

F

F

Q

E

W

D

L

L

R

L

R

E

Q

Q

G

G

G

G

L

L

E

K

A

E

M

V

G

E

K

K

R

K

N

N

Q

Q

A

R

K

K

A

A

A

D

K

L

L

L

Y

Y

E

N

A

A

I

I

D

E

D

E

S

S

S

N

-

G

F

F

Y

F

A

R

N

C

P

P

V

V

A

E

K

K

G

S

A

V

R

R

S

S

W

L

M

M

N

N

V

V

P

P

F

F

T

T

L

L

A

E

R

K

P

S

E

E

L

L

D

E

K

A

T

E

F

F

I

I

A

K

E

E

A

A

R

A

E

K

A

E

G

K

L

M

V

V

T

Q

L

L

E

K

G

G

H

H

R

R

S

S

V

V

G

G

G

G

M

M

R

R

A

A

S

S

L

I

Y

Y

N

N

A

A

M

M

P

P

M

L

E

A

G

G

V

V

D

E

A

K

L

L

V

V

G

A

F

F

M

M

R

K

E

D

F

F

E

Q

R

A

R

K

H

H

A

A

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G76 (= G74), A77 (= A75), T78 (= T76), W103 (= W101), T153 (= T150), D173 (= D171), Q196 (= Q194), K197 (= K195), N238 (= N236), T239 (= T237)

6xdkD Crystal structure of phosphoserine aminotransferase (serc) from stenotrophomonas maltophilia k279a
57% identity, 99% coverage: 2:358/359 of query aligns to 1:358/359 of 6xdkD

query
sites
6xdkD

R

R

V

A

F

F

N

N

F

F

S

S

A

A

G

G

P

P

A

A

V

T

L

L

P

P

L

E

E

S

V

V

L

L

E

R

Q

Q

A

A

R

Q

D

A

E

E

M

M

L

L

D

D

W

W

H

H

G

G

T

S

G

G

M

A

S

S

V

I

M

V

E

E

M

M

S

S

H

H

R

R

G

G

K

A

A

E

F

F

V

M

S

S

I

V

A

A

A

A

Q

E

A

A

E

E

A

A

D

D

L

L

R

R

E

R

L

L

L

L

Q

D

V

I

P

P

A

D

N

D

Y

Y

K

A

V

V

L

L

F

F

L

L

Q

S

G

G

G

G

A

A

T

T

A

T

Q

Q

F

Q

A

A

A

L

I

I

P

P

M

L

N

N

L

F

A

A

G

A

P

P

D

G

S

Q

V

R

V

A

D

D

Y

Y

V

V

H

V

T

S

G

G

A

H

W

W

S

G

K

K

K

T

A

A

I

V

G

K

E

Q

A

A

K

G

R

V

Y

Y

A

V

N

D

V

V

N

N

V

I

A

A

A

A

N

S

A

S

-

E

G

A

E

N

P

G

Y

Y

A

R

S

E

I

L

P

P

A

A

Q

R

E

A

S

D

W

W

Q

Q

R

L

S

S

P

R

N

D

A

A

A

A

Y

Y

L

V

H

H

Y

I

T

T

P

A

N

N

E

E

T

T

I

I

G

H

G

G

V

V

E

E

F

F

H

R

F

D

I

V

P

P

D

D

T

T

N

G

A

-

E

N

V

V

P

P

L

L

V

I

A

A

D

D

L

F

S

S

S

S

T

S

I

I

L

A

S

S

R

E

P

P

L

L

D

D

V

V

S

R

K

R

F

Y

G

G

L

V

I

I

Y

Y

A

A

G

G

A

A

Q

Q

K

K

N

N

I

L

G

G

P

P

A

V

G

G

L

V

A

A

L

V

V

M

I

I

V

I

R

R

E

R

D

D

L

L

L

L

G

E

R

R

A

S

R

G

A

Q

G

P

T

R

P

A

T

D

I

I

L

F

D

D

F

Y

A

R

A

S

M

H

A

V

K

A

D

R

G

D

S

S

M

M

L

L

N
|
N

T
|
T

P

P

P

P

T

T

Y

W

A

N

W

W

Y

Y

V

L

A

A

G

G

L

L

V

V

F

F

Q

K

W

W

L

M

R

L

R

A

Q

E

G

G

G

G

L

V

E

T

A

E

M

F

G

A

K

K

R

R

N

N

Q

A

A

A

K

K

A

A

A

A

K

L

L

V

Y

Y

E

G

A

A

I

I

D

D

D

G

S

S

-

G

S

G

F

F

Y

Y

A

R

N

N

P

E

V

V

A

A

K

Y

G

A

A

A

R

R

S

S

W

R

M

M

N

N

V

I

P

P

F

F

T

F

L

L

A

P

R

D

P

A

E

E

L

L

D

D

K

A

T

R

F

F

I

V

A

A

E

E

A

A

R

K

E

A

A

A

G

G

L

L

V

L

T

A

L

L

E

K

G

G

H

H

R

K

S

V

V

V

G

G

G

G

M

I

R

R

A

A

S

S

L

L

Y

Y

N

N

A

A

M

M

P

P

M

L

E

A

G

G

V

A

D

E

A

A

L

L

V

V

G

A

F

F

M

M

R

A

E

D

F

F

E

Q

R

Q

R

R

H

H

binding pyridoxal-5'-phosphate: N235 (= N236), T236 (= T237)

6xdkB Crystal structure of phosphoserine aminotransferase (serc) from stenotrophomonas maltophilia k279a
56% identity, 99% coverage: 2:358/359 of query aligns to 1:354/355 of 6xdkB

query
sites
6xdkB

R

R

V

A

F

F

N

N

F

F

S

S

A

A

G

G

P

P

A

A

V

T

L

L

P

P

L

E

E

S

V

V

L

L

E

R

Q

Q

A

A

R

Q

D

A

E

E

M

M

L

L

D

D

W

W

H

H

G

G

T

S

G

G

M

A

S

S

V

I

M

V

E

E

M

M

S

S

H

H

R

R

G

G

K

A

A

E

F

F

V

M

S

S

I

V

A

A

A

A

Q

E

A

A

E

E

A

A

D

D

L

L

R

R

E

R

L

L

L

L

Q

D

V

I

P

P

A

D

N

D

Y

Y

K

A

V

V

L

L

F

F

L

L

Q

S

G

G

G

G

A
|
A

T
|
T

A

T

Q

Q

F

Q

A

A

A

L

I

I

P

P

M

L

N

N

L

F

A

A

G

A

P

P

D

G

S

Q

V

R

V

A

D

D

Y

Y

V

V

H

V

T

S

G

G

A

H

W
|
W

S

G

K

K

K

T

A

A

I

V

G

K

E

Q

A

A

K
x
G

R

V

Y

Y

A
x
V

N
x
D

V

V

N

N

V

I

A

A

A

A

N

S

A

S

-

E

G

A

E

N

P

G

Y

Y

A

R

S

E

I

L

P

P

A

A

Q

R

E

A

S

D

W

W

Q

Q

R

L

S

S

P

R

N

D

A

A

A

A

Y

Y

L

V

H

H

Y

I

T

T

P

A

N

N

E

E

T
|
T

I

I

G

H

G

G

V

V

E

E

F

F

H

R

F

D

I

V

P

P

D

D

T

T

N

G

A

-

E

N

V

V

P

P

L

L

V

I

A

A

D
|
D

L

F

S
|
S

S

S

T

S

I

I

L

A

S

S

R

E

P

P

L

L

D

D

V

V

S

R

K

R

F

Y

G

G

L

V

I

I

Y

Y

A

A

G

G

A

A

Q

Q

K
|
K

N

N

I

L

G

G

P

P

A

V

G

G

L

V

A

A

L

V

V

M

I

I

V

I

R

R

E

R

D

D

L

L

L

L

G

E

R

R

A

S

R

G

A

Q

G

P

T

R

P

A

T

D

I

I

L

F

D

D

F

Y

A

-

A

-

M

-

A

-

K

R

D

S

G

H

S

V

M

A

L

R

N

N

T

T

P

P

P

P

T

T

Y

W

A

N

W

W

Y

Y

V

L

A

A

G

G

L

L

V

V

F

F

Q

K

W

W

L

M

R

L

R

A

Q

E

G

G

G

G

L

V

E

T

A

E

M

F

G

A

K

K

R

R

N

N

Q

A

A

A

K

K

A

A

A

A

K

L

L

V

Y

Y

E

G

A

A

I

I

D

D

D

G

S

S

-

G

S

G

F

F

Y

Y

A

R

N

N

P

E

V

V

A

A

K

Y

G

A

A

A

R

R

S

S

W

R

M

M

N

N

V

I

P

P

F

F

T

F

L

L

A

P

R

D

P

A

E

E

L

L

D

D

K

A

T

R

F

F

I

V

A

A

E

E

A

A

R

K

E

A

A

A

G

G

L

L

V

L

T

A

L

L

E

K

G

G

H

H

R

K

S

V

V

V

G

G

G

G

M

I

R

R

A

A

S

S

L

L

Y

Y

N

N

A

A

M

M

P

P

M

L

E

A

G

G

V

A

D

E

A

A

L

L

V

V

G

A

F

F

M

M

R

A

E

D

F

F

E

Q

R

Q

R

R

H

H

binding calcium ion: G109 (≠ K110), V112 (≠ A113), D113 (≠ N114)
binding pyridoxal-5'-phosphate: A74 (= A75), T75 (= T76), W100 (= W101), T150 (= T150), D170 (= D171), S172 (= S173), K194 (= K195)

3qm2B 2.25 angstrom crystal structure of phosphoserine aminotransferase (serc) from salmonella enterica subsp. Enterica serovar typhimurium
54% identity, 99% coverage: 2:358/359 of query aligns to 1:330/331 of 3qm2B

query
sites
3qm2B

R

Q

V

V

F

F

N

N

F

F

S

S

A

S

G

G

P

P

A

A

V

M

L

L

P

P

L

A

E

E

V

V

L

L

E

K

Q

L

A

A

R

Q

D

Q

E

E

M

L

L

-

D

-

W

-

H

-

G

-

T

-

G

-

M

-

S

-

V

-

M

-

E

-

M

-

S

-

H

-

R

-

G

-

K

-

A

-

F

-

V

-

S

-

I

V

A

A

A

E

Q

E

A

A

E

E

A

Q

D

D

L

F

R

R

E

D

L

L

L

L

Q

N

V

I

P

P

A

S

N

N

Y

Y

K

K

V

V

L

L

F

F

L

C

Q

H

G

G

G

G

A

G

T

R

A

G

Q

Q

F

F

A

A

A

G

I

V

P

P

M

L

N

N

L

L

A

L

G

G

P

D

D

K

S

T

V

T

V

A

D

D

Y

Y

V

V

H

D

T

A

G

G

A

Y

W
|
W

S

A

K

A

K

S

A

A

I

I

G

K

E

E

A

A

K

K

R

K

Y

Y

A

C

N

A

-

P

-

Q

-

I

V

I

N

D

V

A

A

K

A

I

N

T

A

V

G

D

E

G

P

K

Y

R

A

A

S

V

I

K

P

P

A

M

Q

R

E

E

S

-

W

W

Q

Q

R

L

S

S

P

D

N

N

A

A

A

A

Y

Y

L

L

H

H

Y

Y

T

C

P

P

N

N

E

E

T

T

I

I

G

D

G

G

V

I

E

A

F

I

H

D

F

E

I

T

P

P

D

D

T

F

N

G

A

P

E

E

V

V

P

V

L

V

V

T

A

A

D
|
D

L

F

S

S

S

S

T

T

I

I

L

L

S

S

R

A

P

P

L

L

D

D

V

V

S

S

K

R

F

Y

G

G

L

V

I

I

Y

Y

A

A

G

G

A

A

Q

Q

K
|
K

N

N

I

I

G

G

P

P

A

A

G

G

L

L

A

T

L

L

V

V

I

I

V

V

R

R

E

E

D

D

L

L

L

L

G

G

R

K

A

A

R

H

A

E

G

S

T

C

P

P

T

S

I

I

L

L

D

D

F

Y

A

-

A

-

M

-

A

-

K

-

D

-

G

-

S

T

M

V

L

L

N

N

T

D

P

N

P

P

T

T

Y

F

A

A

W

W

Y

Y

V

L

A

S

G

G

L

L

V

V

F

F

Q

K

W

W

L

L

R

K

R

A

Q

Q

G

G

G

G

L

V

E

A

A

A

M

M

G

H

K

K

R

I

N

N

Q

Q

A

Q

K

K

A

A

A

E

K

L

L

L

Y

Y

E

G

A

V

I
|
I

D
|
D

D

N

S
|
S

S

D

F

F

Y
|
Y

A

R

N

N

P

D

V

V

A

A

K

Q

G

A

A

N

R

R

S

S

W

R

M

M

N

N

V

V

P

P

F

F

T

Q

L

L

A

A

R

D

P

N

E

T

L

L

D

D

K

K

T

V

F

F

I

L

A

E

E

E

A

S

R

F

E

A

A

A

G

G

L

L

V

H

T

A

L

L

E

K

G

G

H

H

R

R

S

V

V

V

G

G

G

G

M

M

R

R

A

A

S

S

L

I

Y

Y

N

N

A

A

M

M

P

P

M

I

E

E

G

G

V

V

D

K

A

A

L

L

V

T

G

D

F

F

M

M

R

I

E

D

F

F

E

E

R

R

R

R

H

H

active site: W78 (= W101), D150 (= D171), K174 (= K195)
binding calcium ion: I249 (= I277), D250 (= D278), S252 (= S280), Y255 (= Y283)

1bt4A Phosphoserine aminotransferase from bacillus circulans subsp. Alkalophilus
50% identity, 99% coverage: 2:356/359 of query aligns to 3:358/361 of 1bt4A

query
sites
1bt4A

R

R

V

A

F

Y

N

N

F

F

S

N

A

A

G

G

P

P

A

A

V

A

L

L

P

P

L

L

E

E

V

V

L

L

E

E

Q

R

A

A

R

Q

D

A

E

E

M

F

L

V

D

D

W

Y

H

Q

G

H

T

T

G

G

M

M

S

S

V

I

M

M

E

E

M

M

S

S

H

H

R

R

G

G

K

A

A

V

F

Y

V

E

S

A

I

V

A

H

A

N

Q

E

A

A

E

Q

A

A

D

R

L

L

R

L

E

A

L

L

L

L

Q

G

V

N

P

P

A

T

N

G

Y

Y

K

K

V

V

L

L

F

F

L

I

Q

Q

G

G

G

G

A
|
A

T
x
S

A

T

Q

Q

F

F

A

A

A

M

I

I

P

P

M

M

N

N

L

F

A

L

G

K

P

E

D

G

S

Q

V

T

V

A

D

N

Y

Y

V

V

H

M

T

T

G

G

A

S

W
|
W

S

A

K

S

K

K

A

A

I

L

G

K

E

E

A

A

K

K

R

L

Y

I

A

G

N

D

V

T

N

H

V

V

A

A

A

A

N

S

A

S

-

E

G

A

E

S

P

N

Y

Y

A

M

S

T

I

L

P

P

A

K

Q

L

E

Q

S

E

W

I

Q

Q

R

L

S

Q

P

D

N

N

A

A

A

A

Y

Y

L

L

H

H

Y

L

T

T

P

S

N

N

E

E

T

T

I

I

G

E

G

G

V

A

E

Q

F

F

H

K

F

A

I

F

P

P

D

D

T

T

N

G

A

S

E

-

V

V

P

P

L

L

V

I

A

G

D
|
D

L

M

S
|
S

S

S

T

D

I

I

L

L

S

S

R

R

P

P

L

F

D

D

V

L

S

N

K

Q

F

F

G

G

L

L

I

V

Y

Y

A

A

G

G

A

A

Q
|
Q

K
|
K

N

N

I

L

G

G

P

P

A

S

G

G

L

V

A

T

L

V

V

V

I

I

V

V

R

R

E

E

D

D

L

L

L

V

G

A

R

E

A

S

R

P

A

K

G

H

T

L

P

P

T

T

I

M

L

L

D

R

F

Y

A

D

A

T

M

Y

A

V

K

K

D

N

G

N

S

S

M

L

L

Y

N

N

T

T

P

P

P

P

T

S

Y

F

A

G

W

I

Y

Y

V

M

A

V

G

N

L

E

V

V

F

L

Q

K

W

W

L

I

R

E

R

E

Q

R

G

G

G

G

L

L

E

E

A

G

M

V

G

Q

K

Q

R

A

N

N

Q

R

A

K

K

K

A

A

A

S

K

L

L

I

Y

Y

E

D

A

A

I

I

D

D

D

Q

S

S

-

G

S

G

F

F

Y

Y

A

R

N

G

P

C

V

V

A

D

K

V

G

D

A

S

R

R

S

S

W

D

M

M

N

N

V

I

P

T

F

F

T

R

L

L

A

A

R

S

P

E

E

E

L

L

D

E

K

K

T

E

F

F

I

V

A

K

E

A

A

S

R

E

E

Q

A

E

G

G

L

F

V

V

T

G

L

L

E

K

G

G

H

H

R

R

S

S

V

V

G

G

G

G

M

L

R

R

A

A

S

S

L

I

Y

Y

N

N

A

A

M

V

P

P

M

Y

E

E

G

S

V

C

D

E

A

A

L

L

V

V

G

Q

F

F

M

M

R

E

E

H

F

F

E

K

R

R

active site: W102 (= W101), D172 (= D171), K196 (= K195)
binding pyridoxal-5'-phosphate: A76 (= A75), S77 (≠ T76), W102 (= W101), D172 (= D171), S174 (= S173), Q195 (= Q194), K196 (= K195)

8a5wC Crystal structure of the human phosphoserine aminotransferase (psat) in complex with o-phosphoserine (see paper)
48% identity, 99% coverage: 2:358/359 of query aligns to 1:363/365 of 8a5wC

query
sites
8a5wC

R

Q

V

V

F

V

N

N

F

F

S

G

A

P

G

G

P

P

A

A

V

K

L

L

P

P

L

H

E

S

V

V

L

L

E

L

Q

E

A

I

R

Q

D

K

E

E

M

L

L

L

D

D

W

Y

H

K

G

G

T

V

G

G

M

I

S

S

V

V

M

L

E

E

M

M

S

S

H
|
H

R
|
R

G

S

K

S

A

D

F

F

V

A

S

K

I

I

A

I

A

N

Q

N

A

T

E

E

A

N

D

L

L

V

R

R

E

E

L

L

L

L

Q

A

V

V

P

P

A

D

N

N

Y

Y

K

K

V

V

L

I

F

F

L

L

Q

Q

G

G

G

G

A

G

T

C

A

G

Q

Q

F

F

A

S

A

A

I

V

P

P

M

L

N

N

L

L

A

I

G

G

P

L

D

K

S

A

-

G

-

R

V

C

V

A

D

D

Y

Y

V

V

H

V

T

T

G

G

A

A

W

W

S

S

K

A

K

K

A

A

I

A

G

E

E

E

A

A

K

K

R

K

Y

F

A

G

N

T

V

I

N

N

V

I

A

V

A

H

N

P

A

K

G

L

E

G

P

S

Y

Y

A

T

S

K

I

I

P

P

A

D

Q

P

E

S

S

T

W

W

Q

N

R

L

S

N

P

P

N

D

A

A

A

S

Y

Y

L

V

H

Y

Y

Y

T

C

P

A

N

N

E

E

T
|
T

I

V

G

H

G

G

V

V

E

E

F

F

H

D

F

F

I

I

P

P

D

D

T

V

N

K

A

G

E

A

V

V

P

-

L

L

V

V

A

C

D

D

L

M

S

S

S

S

T

N

I

F

L

L

S

S

R

K

P

P

L

V

D

D

V

V

S

S

K

K

F

F

G

G

L

V

I

I

Y

F

A

A

G

G

A

A

Q

Q

K
|
K

N

N

I

V

G

G

P

S

A

A

G

G

L

V

A

T

L

V

V

V

I

I

V

V

R

R

E

D

D

D

L

L

L

L

G

G

R

F

A

A

R

L

A

R

G

E

T

C

P

P

T

S

I

V

L

L

D

E

F

Y

A

K

A

V

M

Q

A

A

K

G

D

N

G

S

S

S

M

L

L

Y

N
|
N

T
|
T

P

P

P

P

T

C

Y

F

A

S

W

I

Y

Y

V

V

A

M

G

G

L

L

V

V

F

L

Q

E

W

W

L

I

R

K

R

N

Q

N

G

G

G

G

L

A

E

A

A

A

M

M

G

E

K

K

R

L

N

S

Q

S

A

I

K

K

A

S

A

Q

K

T

L

I

Y

Y

E

E

A

I

I

I

D

D

D

N

S

S

S

Q

-

G

F

F

Y

Y

A

V

N

C

P

P

V

V

A

E

K

P

G

Q

A

N

R

R

S

S

W

K

M

M

N

N

V

I

P

P

F

F

T

R

L

I

A

G

R

N

P

A

E

K

-

G

-

D

-

D

-

A

L

L

D

E

K

K

T

R

F

F

I

L

A

D

E

K

A

A

R

L

E

E

A

L

G

N

L

M

V

L

T

S

L

L

E

K

G

G

H
|
H

R
|
R

S

S

V

V

G

G

G

G

M

I

R
|
R

A

A

S

S

L

L

Y

Y

N

N

A

A

M

V

P

T

M

I

E

E

G

D

V

V

D

Q

A

K

L

L

V

A

G

A

F

F

M

M

R

K

E

K

F

F

E

L

R

E

R

M

H

H

binding (2S)-2-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-3-phosphonooxy-propanoic acid: H39 (= H40), R40 (= R41), N236 (= N236), T237 (= T237)
binding phosphoserine: T151 (= T150), K195 (= K195), H330 (= H325), R331 (= R326), R337 (= R332)

8a5wA Crystal structure of the human phosphoserine aminotransferase (psat) in complex with o-phosphoserine (see paper)
48% identity, 99% coverage: 2:358/359 of query aligns to 1:363/365 of 8a5wA

query
sites
8a5wA

R

Q

V

V

F

V

N

N

F

F

S

G

A

P

G

G

P

P

A

A

V

K

L

L

P

P

L

H

E

S

V

V

L

L

E

L

Q

E

A

I

R

Q

D

K

E

E

M

L

L

L

D

D

W

Y

H

K

G

G

T

V

G

G

M

I

S

S

V

V

M

L

E

E

M

M

S

S

H
|
H

R
|
R

G

S

K

S

A

D

F

F

V

A

S

K

I

I

A

I

A

N

Q

N

A

T

E

E

A

N

D

L

L

V

R

R

E

E

L

L

L

L

Q

A

V

V

P

P

A

D

N

N

Y

Y

K

K

V

V

L

I

F

F

L

L

Q

Q

G

G

G
|
G

A
x
G

T
x
C

A

G

Q

Q

F

F

A

S

A

A

I

V

P

P

M

L

N

N

L

L

A

I

G

G

P

L

D

K

S

A

-

G

-

R

V

C

V

A

D

D

Y

Y

V

V

H

V

T

T

G

G

A

A

W
|
W

S

S

K

A

K

K

A

A

I

A

G

E

E

E

A

A

K

K

R

K

Y

F

A

G

N

T

V

I

N

N

V

I

A

V

A

H

N

P

A

K

G

L

E

G

P

S

Y

Y

A

T

S

K

I

I

P

P

A

D

Q

P

E

S

S

T

W

W

Q

N

R

L

S

N

P

P

N

D

A

A

A

S

Y

Y

L

V

H

Y

Y

Y

T

C

P

A

N

N

E

E

T
|
T

I

V

G

H

G

G

V

V

E

E

F

F

H

D

F

F

I

I

P

P

D

D

T

V

N

K

A

G

E

A

V

V

P

-

L

L

V

V

A

C

D
|
D

L

M

S
|
S

S

S

T

N

I

F

L

L

S

S

R

K

P

P

L

V

D

D

V

V

S

S

K

K

F

F

G

G

L

V

I

I

Y

F

A

A

G

G

A

A

Q
|
Q

K
|
K

N

N

I

V

G

G

P

S

A

A

G

G

L

V

A

T

L

V

V

V

I

I

V

V

R

R

E

D

D

D

L

L

L

L

G

G

R

F

A

A

R

L

A

R

G

E

T

C

P

P

T

S

I

V

L

L

D

E

F

Y

A

K

A

V

M

Q

A

A

K

G

D

N

G

S

S

S

M

L

L

Y

N

N

T

T

P

P

P

P

T

C

Y

F

A

S

W

I

Y

Y

V

V

A

M

G

G

L

L

V

V

F

L

Q

E

W

W

L

I

R

K

R

N

Q

N

G

G

G

G

L

A

E

A

A

A

M

M

G

E

K

K

R

L

N

S

Q

S

A

I

K

K

A

S

A

Q

K

T

L

I

Y

Y

E

E

A

I

I

I

D

D

D

N

S

S

S

Q

-

G

F

F

Y

Y

A

V

N

C

P

P

V

V

A

E

K

P

G

Q

A

N

R

R

S

S

W

K

M

M

N

N

V

I

P

P

F

F

T

R

L

I

A

G

R

N

P

A

E

K

-

G

-

D

-

D

-

A

L

L

D

E

K

K

T

R

F

F

I

L

A

D

E

K

A

A

R

L

E

E

A

L

G

N

L

M

V

L

T

S

L

L

E

K

G

G

H
|
H

R

R

S

S

V

V

G

G

G

G

M

I

R
|
R

A

A

S

S

L

L

Y

Y

N

N

A

A

M

V

P

T

M

I

E

E

G

D

V

V

D

Q

A

K

L

L

V

A

G

A

F

F

M

M

R

K

E

K

F

F

E

L

R

E

R

M

H

H

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G73 (= G74), G74 (≠ A75), C75 (≠ T76), W102 (= W101), T151 (= T150), D171 (= D171), S173 (= S173), Q194 (= Q194), K195 (= K195)
binding phosphoserine: H39 (= H40), R40 (= R41), H330 (= H325), R337 (= R332)

8a5vA Crystal structure of the human phosposerine aminotransferase (psat) (see paper)
48% identity, 99% coverage: 2:358/359 of query aligns to 1:363/365 of 8a5vA

query
sites
8a5vA

R

Q

V

V

F

V

N

N

F

F

S

G

A

P

G

G

P

P

A

A

V

K

L

L

P

P

L

H

E

S

V

V

L

L

E

L

Q

E

A

I

R

Q

D

K

E

E

M

L

L

L

D

D

W

Y

H

K

G

G

T

V

G

G

M

I

S

S

V

V

M

L

E

E

M

M

S

S

H

H

R

R

G

S

K

S

A

D

F

F

V

A

S

K

I

I

A

I

A

N

Q

N

A

T

E

E

A

N

D

L

L

V

R

R

E

E

L

L

L

L

Q

A

V

V

P

P

A

D

N

N

Y

Y

K

K

V

V

L

I

F

F

L

L

Q

Q

G

G

G

G

A

G

T

C

A

G

Q

Q

F

F

A

S

A

A

I

V

P

P

M

L

N

N

L

L

A

I

G

G

P

L

D

K

S

A

-

G

-

R

V

C

V

A

D

D

Y

Y

V

V

H

V

T

T

G

G

A

A

W

W

S

S

K

A

K

K

A

A

I

A

G

E

E

E

A

A

K

K

R

K

Y

F

A

G

N

T

V

I

N

N

V

I

A

V

A

H

N

P

A

K

G

L

E

G

P

S

Y

Y

A

T

S

K

I

I

P

P

A

D

Q

P

E

S

S

T

W

W

Q

N

R

L

S

N

P

P

N

D

A

A

A

S

Y

Y

L

V

H

Y

Y

Y

T

C

P

A

N

N

E

E

T

T

I

V

G

H

G

G

V

V

E

E

F

F

H

D

F

F

I

I

P

P

D

D

T

V

N

K

A

G

E

A

V

V

P

-

L

L

V

V

A

C

D

D

L

M

S

S

S

S

T

N

I

F

L

L

S

S

R

K

P

P

L

V

D

D

V

V

S

S

K

K

F

F

G

G

L

V

I

I

Y

F

A

A

G

G

A

A

Q

Q

K

K

N

N

I

V

G

G

P

S

A

A

G

G

L

V

A

T

L

V

V

V

I

I

V

V

R

R

E

D

D

D

L

L

L

L

G

G

R

F

A

A

R

L

A

R

G

E

T

C

P

P

T

S

I

V

L

L

D

E

F

Y

A

K

A

V

M

Q

A

A

K

G

D

N

G

S

S

S

M

L

L