SitesBLAST
Comparing WP_243397451.1 NCBI__GCF_000017845.1:WP_243397451.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3s2fE Crystal structure of furx nadh:furfural
34% identity, 98% coverage: 6:331/333 of query aligns to 2:336/340 of 3s2fE
- active site: C40 (= C44), H41 (≠ R45), T42 (= T46), H45 (= H49), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), Q112 (≠ R115), C150 (= C153), T154 (≠ I157), R333 (≠ A328)
- binding furfural: T42 (= T46), W51 (≠ L55), H63 (= H66), W89 (= W92), C150 (= C153), I287 (≠ A282)
- binding nicotinamide-adenine-dinucleotide: C40 (= C44), H41 (≠ R45), T42 (= T46), C150 (= C153), T154 (≠ I157), G174 (= G175), G176 (= G177), G177 (≠ A178), L178 (≠ A179), D197 (≠ T198), I198 (≠ R199), K202 (≠ G205), T239 (≠ P242), A240 (≠ V243), V241 (vs. gap), N262 (≠ A257), G263 (= G258), L264 (≠ I259), I287 (≠ A282), V288 (≠ N283), R333 (≠ A328)
- binding zinc ion: C40 (= C44), H63 (= H66), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), C150 (= C153)
3s2fA Crystal structure of furx nadh:furfural
34% identity, 98% coverage: 6:331/333 of query aligns to 2:336/340 of 3s2fA
- active site: C40 (= C44), H41 (≠ R45), T42 (= T46), H45 (= H49), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), Q112 (≠ R115), C150 (= C153), T154 (≠ I157), R333 (≠ A328)
- binding phosphorylisopropane: T42 (= T46), H63 (= H66), W89 (= W92), I287 (≠ A282)
- binding zinc ion: C40 (= C44), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), C150 (= C153)
3s2eE Crystal structure of furx nadh complex 1
34% identity, 98% coverage: 6:331/333 of query aligns to 2:336/340 of 3s2eE
- active site: C40 (= C44), H41 (≠ R45), T42 (= T46), H45 (= H49), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), Q112 (≠ R115), C150 (= C153), T154 (≠ I157), R333 (≠ A328)
- binding nicotinamide-adenine-dinucleotide: C40 (= C44), H41 (≠ R45), T42 (= T46), C150 (= C153), T154 (≠ I157), G176 (= G177), G177 (≠ A178), L178 (≠ A179), D197 (≠ T198), I198 (≠ R199), K202 (≠ G205), T239 (≠ P242), A240 (≠ V243), V241 (vs. gap), S242 (vs. gap), A245 (vs. gap), N262 (≠ A257), G263 (= G258), L264 (≠ I259), I287 (≠ A282), V288 (≠ N283)
- binding zinc ion: C40 (= C44), H63 (= H66), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), C150 (= C153)
3s2eA Crystal structure of furx nadh complex 1
34% identity, 98% coverage: 6:331/333 of query aligns to 2:336/340 of 3s2eA
- active site: C40 (= C44), H41 (≠ R45), T42 (= T46), H45 (= H49), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), Q112 (≠ R115), C150 (= C153), T154 (≠ I157), R333 (≠ A328)
- binding zinc ion: C40 (= C44), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), C150 (= C153)
6iqdA Crystal structure of alcohol dehydrogenase from geobacillus stearothermophilus (see paper)
35% identity, 98% coverage: 7:331/333 of query aligns to 1:334/336 of 6iqdA
- active site: C38 (= C44), T40 (= T46), H43 (= H49), H61 (= H66), C148 (= C153)
- binding zinc ion: C38 (= C44), H61 (= H66), E62 (= E67), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), C148 (= C153)
3meqA Crystal structure of alcohol dehydrogenase from brucella melitensis
36% identity, 98% coverage: 5:331/333 of query aligns to 1:338/341 of 3meqA
- active site: C40 (= C44), H41 (≠ R45), T42 (= T46), H45 (= H49), H63 (= H66), E64 (= E67), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), L112 (≠ R115), C150 (= C153), T154 (≠ I157), R335 (≠ A328)
- binding 1,4-dihydronicotinamide adenine dinucleotide: C40 (= C44), H41 (≠ R45), T42 (= T46), H45 (= H49), C150 (= C153), T154 (≠ I157), G176 (= G177), G177 (≠ A178), L178 (≠ A179), D197 (≠ T198), I198 (≠ R199), K202 (≠ G205), T241 (≠ G238), A242 (= A239), V243 (≠ L240), S244 (≠ V241), A247 (vs. gap), N264 (≠ A257), G265 (= G258), L266 (≠ I259), I289 (≠ A282), V290 (≠ N283)
- binding zinc ion: C40 (= C44), H63 (= H66), C94 (= C97), C97 (= C100), C100 (= C103), C108 (= C111), C150 (= C153)
P12311 Alcohol dehydrogenase; ADH-T; EC 1.1.1.1 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
34% identity, 98% coverage: 7:331/333 of query aligns to 1:334/337 of P12311
- C38 (= C44) mutation to S: No activity.
- T40 (= T46) mutation to A: No activity.; mutation to S: Little decrease in activity.
- H43 (= H49) mutation to A: No activity.; mutation to R: Higher level of activity at pH 9.
1rjwA Crystal structure of NAD(+)-dependent alcohol dehydrogenase from bacillus stearothermophilus strain lld-r (see paper)
34% identity, 98% coverage: 7:331/333 of query aligns to 1:334/339 of 1rjwA
- active site: C38 (= C44), H39 (≠ R45), T40 (= T46), H43 (= H49), H61 (= H66), E62 (= E67), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), K110 (≠ R115), C148 (= C153), T152 (≠ I157), R331 (≠ A328)
- binding trifluoroethanol: T40 (= T46), C148 (= C153), I285 (≠ V281)
- binding zinc ion: C38 (= C44), H61 (= H66), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111)
Q8GIX7 Alcohol dehydrogenase; ADH; EC 1.1.1.1 from Moraxella sp. (strain TAE123) (see paper)
34% identity, 98% coverage: 7:331/333 of query aligns to 1:334/338 of Q8GIX7
- C38 (= C44) binding Zn(2+)
- H61 (= H66) binding Zn(2+)
- E62 (= E67) binding Zn(2+)
- C92 (= C97) binding Zn(2+)
- C95 (= C100) binding Zn(2+)
- C98 (= C103) binding Zn(2+)
- C106 (= C111) binding Zn(2+)
- C148 (= C153) binding Zn(2+)
3piiA Crystal structure of mutant of ht- alcohol dehydrogenase with substrate analogue butyramide
34% identity, 98% coverage: 7:331/333 of query aligns to 1:334/337 of 3piiA
- active site: C38 (= C44), H39 (≠ R45), T40 (= T46), H43 (= H49), H61 (= H66), E62 (= E67), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), K110 (≠ R115), C148 (= C153), T152 (≠ I157), R331 (≠ A328)
- binding butyramide: T40 (= T46), H61 (= H66), W87 (= W92), C148 (= C153)
- binding zinc ion: C38 (= C44), H61 (= H66), E62 (= E67), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), C148 (= C153)
4z6kA Alcohol dehydrogenase from the antarctic psychrophile moraxella sp. Tae 123
34% identity, 98% coverage: 7:331/333 of query aligns to 1:334/345 of 4z6kA
- active site: C38 (= C44), H39 (≠ R45), T40 (= T46), H43 (= H49), H61 (= H66), E62 (= E67), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), Q110 (≠ R115), C148 (= C153), T152 (≠ I157), R331 (≠ A328)
- binding zinc ion: C38 (= C44), H61 (= H66), C92 (= C97), C95 (= C100), C98 (= C103), C106 (= C111), C148 (= C153)
1lluA The ternary complex of pseudomonas aeruginosa alcohol dehydrogenase with its coenzyme and weak substrate (see paper)
33% identity, 98% coverage: 5:331/333 of query aligns to 4:339/341 of 1lluA
- active site: C43 (= C44), H44 (≠ R45), T45 (= T46), H48 (= H49), H66 (= H66), E67 (= E67), C97 (= C97), C100 (= C100), C103 (= C103), C111 (= C111), Q115 (≠ R115), C153 (= C153), T157 (≠ I157), R336 (≠ A328)
- binding 1,2-ethanediol: H44 (≠ R45), T45 (= T46), L47 (= L48), D53 (≠ E54), W92 (= W92), C153 (= C153)
- binding nicotinamide-adenine-dinucleotide: C43 (= C44), H44 (≠ R45), T45 (= T46), H48 (= H49), C153 (= C153), T157 (≠ I157), G179 (= G177), G180 (≠ A178), L181 (≠ A179), D200 (≠ T198), I201 (≠ R199), K205 (≠ G205), A243 (= A235), V244 (≠ P236), S245 (≠ V237), A248 (≠ L240), V265 (≠ A257), L267 (≠ I259), I290 (≠ A282), V291 (≠ N283), R336 (≠ A328)
- binding zinc ion: C43 (= C44), H66 (= H66), C100 (= C100), C103 (= C103), C111 (= C111), C153 (= C153)
6n7lC Crystal structure of an alcohol dehydrogenase from elizabethkingia anophelis nuhp1
32% identity, 99% coverage: 3:331/333 of query aligns to 4:340/344 of 6n7lC
- active site: C45 (= C44), T47 (= T46), H50 (= H49), H68 (= H66), C154 (= C153)
- binding nicotinamide-adenine-dinucleotide: C45 (= C44), H46 (≠ R45), T47 (= T46), H50 (= H49), C154 (= C153), T158 (≠ I157), G178 (= G175), G180 (= G177), G181 (≠ A178), L182 (≠ A179), D201 (≠ T198), V202 (≠ R199), K206 (≠ D203), T243 (≠ G238), A244 (= A239), V245 (≠ L240), S246 (≠ V241), A249 (= A244), N266 (≠ S262), G267 (≠ D263), L268 (≠ I264), I291 (vs. gap), V292 (vs. gap)
- binding zinc ion: C45 (= C44), H68 (= H66), C98 (= C97), C101 (= C100), C104 (= C103), C112 (= C111), C154 (= C153)
6z42A The low resolution structure of a zinc-dependent alcohol dehydrogenase from halomonas elongata.
33% identity, 96% coverage: 7:325/333 of query aligns to 4:328/336 of 6z42A
- active site: C41 (= C44), T43 (= T46), H46 (= H49), H64 (= H66), C148 (= C153)
- binding zinc ion: C41 (= C44), H64 (= H66), E65 (= E67), C95 (= C97), C98 (= C100), C101 (= C103), C109 (= C111), C148 (= C153)
4eezB Crystal structure of lactococcus lactis alcohol dehydrogenase variant re1 (see paper)
28% identity, 93% coverage: 22:331/333 of query aligns to 20:336/342 of 4eezB
- active site: C39 (= C44), H40 (≠ R45), T41 (= T46), H44 (= H49), H60 (= H66), E61 (= E67), C91 (= C97), C94 (= C100), C97 (= C103), C105 (= C111), K109 (≠ R115), C147 (= C153), T151 (≠ I157), R333 (≠ A328)
- binding zinc ion: C39 (= C44), H60 (= H66), E61 (= E67), C91 (= C97), C94 (= C100), C97 (= C103), C105 (= C111), C147 (= C153)
2eerB Structural study of project id st2577 from sulfolobus tokodaii strain7
31% identity, 98% coverage: 7:333/333 of query aligns to 1:347/347 of 2eerB
- active site: C38 (= C44), H39 (≠ R45), S40 (≠ T46), H43 (= H49), H68 (= H66), E69 (= E67), E98 (≠ G95), C101 (= C100), C104 (= C103), C112 (= C111), R116 (= R115), C154 (= C153), T158 (≠ I157), R342 (≠ A328)
- binding nicotinamide-adenine-dinucleotide: C38 (= C44), H39 (≠ R45), S40 (≠ T46), H43 (= H49), C154 (= C153), T158 (≠ I157), G178 (= G177), G181 (≠ S180), G182 (≠ H181), L183 (= L183), D203 (= D203), V204 (≠ L204), R205 (≠ G205), L247 (≠ F234), N248 (≠ A235), V270 (≠ A257), G271 (= G258), L272 (≠ I259), F273 (≠ H260), L295 (≠ V281), V296 (≠ A282), R342 (≠ A328)
- binding zinc ion: E98 (≠ G95), C101 (= C100), C104 (= C103), C112 (= C111)
P00331 Alcohol dehydrogenase 2; Alcohol dehydrogenase II; ADHII; YADH-2; EC 1.1.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
35% identity, 49% coverage: 1:163/333 of query aligns to 1:164/348 of P00331
- M1 (= M1) modified: Initiator methionine, Removed
- S2 (≠ E2) modified: N-acetylserine
4gkvB Structure of escherichia coli adhp (ethanol-inducible dehydrogenase) with bound NAD (see paper)
31% identity, 90% coverage: 33:331/333 of query aligns to 26:332/336 of 4gkvB
- active site: C37 (= C44), H38 (≠ R45), T39 (= T46), H42 (= H49), H58 (= H66), E59 (= E67), C89 (= C97), C92 (= C100), C95 (= C103), C103 (= C111), K107 (≠ R115), C145 (= C153), T149 (≠ I157), R329 (≠ A328)
- binding nicotinamide-adenine-dinucleotide: C37 (= C44), H38 (≠ R45), T39 (= T46), H42 (= H49), C145 (= C153), T149 (≠ I157), G169 (= G175), G171 (= G177), G172 (≠ A178), L173 (≠ A179), D193 (≠ T198), V194 (vs. gap), Q198 (vs. gap), T235 (≠ F234), A236 (= A235), V237 (≠ P236), V258 (≠ A257), G259 (= G258), L260 (≠ I259), L283 (≠ V281), V284 (≠ A282), R329 (≠ A328)
- binding zinc ion: C37 (= C44), H58 (= H66), C89 (= C97), C92 (= C100), C95 (= C103), C103 (= C111), C145 (= C153)
- binding : Q223 (= Q222), D247 (≠ K246), R271 (≠ S266), L274 (= L272)
P00330 Alcohol dehydrogenase 1; Alcohol dehydrogenase I; ADHI; NADH-dependent methylglyoxal reductase; YADH-1; EC 1.1.1.1; EC 1.1.1.54; EC 1.1.1.78 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
30% identity, 64% coverage: 1:213/333 of query aligns to 1:215/348 of P00330
- M1 (= M1) modified: Initiator methionine, Removed
- S2 (≠ E2) modified: N-acetylserine
- C44 (= C44) binding Zn(2+)
- H45 (≠ R45) binding NAD(+); mutation to R: Decreases dissociation constants by 4-fold for NAD(+) and 2-fold for NADH, while turnover numbers were decreased by 4-fold for ethanol oxidation and 6-fold for acetaldehyde reduction.
- T46 (= T46) binding NAD(+); mutation to S: Has the same pattern of activity as the wild-type enzyme for linear primary alcohols.
- H49 (= H49) binding NAD(+)
- W55 (≠ L55) mutation to M: Has lowered reactivity with primary and secondary alcohols.
- H67 (= H66) binding Zn(2+)
- E68 (= E67) binding in the open conformation
- W93 (= W92) mutation to A: Has an inverted specificity pattern for primary alcohols, being 3- and 10-fold more active on hexanol and 350- and 540-fold less active on ethanol. Also acquires weak activity on branched chain alcohols and cyclohexanol.
- C98 (= C97) binding Zn(2+)
- C101 (= C100) binding Zn(2+)
- C104 (= C103) binding Zn(2+)
- C112 (= C111) binding Zn(2+)
- C154 (= C153) binding Zn(2+)
- G181 (= G177) binding NAD(+)
- G182 (≠ A178) binding NAD(+)
- L183 (≠ A179) binding NAD(+)
- D202 (≠ T198) binding NAD(+)
- K207 (≠ G205) binding NAD(+)
Sites not aligning to the query:
- 222 binding NAD(+)
- 236 natural variant: T -> I
- 269 binding NAD(+)
- 271 binding NAD(+); M→L: Produces a 7 to 10-fold increase in reactivity with butanol, pentanol, and hexanol.
- 294 binding NAD(+)
- 296 binding NAD(+)
- 341 binding NAD(+)
5yatA Crystal structure of mitochondrial alcohol dehydrogenase isozyme iii from komagataella phaffii gs115 (see paper)
35% identity, 48% coverage: 3:163/333 of query aligns to 2:163/347 of 5yatA
- active site: C43 (= C44), T45 (= T46), H48 (= H49), H66 (= H66), C153 (= C153)
- binding zinc ion: C43 (= C44), H66 (= H66), E67 (= E67), C97 (= C97), C100 (= C100), C103 (= C103), C111 (= C111), C153 (= C153)
Query Sequence
>WP_243397451.1 NCBI__GCF_000017845.1:WP_243397451.1
MEVKQMMRAMVLEAAKEKLRLELRPIPQPDDKQLLLKIQACGVCRTDLHIVDGELTQAKF
PLILGHEIVGTVEAVGKGVKRFSLGERVGVPWLGGTCQHCRYCRTQQENLCEQARFTGYH
LDGGYAEYTVANEQFCFAIPKRYSDLEVAPLLCAGLIGYRSYRLVGDGEKIGFYGFGAAS
HILLQVARHQGRQVYAFTRPGDDLGQDFARSLGAVWAGGSEQSPPDSLDGAIIFAPVGAL
VPVALKAVVPGGVVVCAGIHMSDIPSFPYQILWQERVLRSVANLTRQDGEDFFALARQIP
IQTQVSSFPLTQANVALDCLRQGKIKGAAVLVP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory