SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_244155490.1 NCBI__GCF_000422285.1:WP_244155490.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
53% identity, 91% coverage: 15:235/243 of query aligns to 3:222/226 of 5xu1B

query
sites
5xu1B

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binding magnesium ion: G45 (= G57), K46 (= K58), S47 (= S59)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
47% identity, 93% coverage: 15:240/243 of query aligns to 1:230/232 of 1f3oA

query
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1f3oA

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binding adenosine-5'-diphosphate: Y11 (= Y25), G41 (= G55), G43 (= G57), K44 (= K58), S45 (= S59), T46 (= T60)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
47% identity, 93% coverage: 15:240/243 of query aligns to 1:230/230 of 1l2tA

query
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1l2tA

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binding adenosine-5'-triphosphate: Y11 (= Y25), S40 (= S54), G41 (= G55), S42 (= S56), G43 (= G57), K44 (= K58), S45 (= S59), T46 (= T60), F138 (vs. gap), Q145 (≠ E155), S147 (= S157), G149 (= G159), Q150 (= Q160), H204 (= H214)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
47% identity, 95% coverage: 12:242/243 of query aligns to 1:225/648 of P75831

query
sites
P75831

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K47 (= K58) mutation to L: Lack of activity.
D169 (= D180) mutation to N: Lack of activity.

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
46% identity, 93% coverage: 15:240/243 of query aligns to 3:227/592 of 5lj7A

query
sites
5lj7A

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binding adenosine-5'-triphosphate: V22 (≠ A34), S42 (= S54), G43 (= G55), G45 (= G57), K46 (= K58), S47 (= S59), T48 (= T60), Q92 (= Q104), K136 (≠ W148), Q143 (≠ E155), S145 (= S157), G147 (= G159), Q148 (= Q160)
binding magnesium ion: S47 (= S59), Q92 (= Q104)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
46% identity, 93% coverage: 15:240/243 of query aligns to 3:227/615 of 5lilA

query
sites
5lilA

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V

S

F

S

L

K

F

I

D

D

G

G

V

K

D

E

V

T

A

I

R

E

L

L

T

T

R

N

D

D

Q

Q

R

L

A

S

L

D

L

L

R

R

R

S

H

Q

D

K

L

F

G

G

F

F

V

I

F

F

Q
|
Q

G

R

F

Y

N

N

L

L

L

L

S

S

R

S

T

L

S

T

A

A

L

A

E

E

N

N

V

V

E

A

L

L

P

P

L

A

I

I

Y

Y

R

A

R

G

V

M

P

P

A

Q

A

S

R

Q

R

R

R

L

E

E

L

R

A

A

L

K

R

Q

A

L

L

L

A

E

A

K

V

L

G

G

L

L

S

G

G

D

W
x
K

E

W

S

Q

H

N

T

K

P

P

A

N

E
x
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

T

A

R

R

A

A

I

L

V

M

T

N

E

G

P

G

K

E

V

I

L

I

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

S

S

A

H

L

S

S

G

R

E

E

N

V

V

M

M

E

E

L

I

L

L

Q

R

S

Q

F

L

N

H

R

E

S

-

R

E

G

G

L

H

T

T

I

I

V

I

M

M

V

V

T

T

H

H

E

D

A

K

D

H

M

I

A

A

A

A

Y

S

A

A

E

N

R

R

R

I

I

I

L

E

F

I

R

K

D

D

G

G

R

E

I

I

A

I

S

S

I

D

E

T

T

Q

E

K

R

R

binding adenosine-5'-triphosphate: F13 (≠ Y25), V22 (≠ A34), S42 (= S54), G43 (= G55), G45 (= G57), K46 (= K58), S47 (= S59), T48 (= T60), Q92 (= Q104), K136 (≠ W148), Q143 (≠ E155), S145 (= S157), G147 (= G159), Q148 (= Q160)
binding magnesium ion: S47 (= S59), Q92 (= Q104)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
47% identity, 92% coverage: 13:235/243 of query aligns to 2:223/650 of 5ws4A

query
sites
5ws4A

E

Q

P

A

I

L

I

L

R

E

L

V

T

S

N

N

V

L

T

V

K

R

T

E

Y

F

G

P

S

A

G

G

S

E

A

S

A

T

M

I

Q

Q

A

I

L

L

K

K

G

G

I

I

D

D

L

L

A

T

I

I

H

Y

P

E

G

G

E

E

F

L

V

V

A

A

V

I

M

V

G

G

P

Q

S
|
S

G
|
G

S

S

G

G

K

K

S
|
S

T

T

C

L

M

M

N

N

I

I

L

L

G

G

C

C

L

L

D

D

T

R

P

P

S

T

S

S

G

G

V

S

F

Y

L

K

F

V

D

N

G

G

V

Q

D

E

V

T

A

G

R

K

L

L

T

E

R

P

D

D

Q

Q

R

L

A

A

L

Q

L

L

R

R

R

R

H

E

D

Y

L

F

G

G

F

F

V

I

F

F

Q

Q

G

R

F

Y

N

H

L

L

L

L

S

G

R

D

T

L

S

S

A

A

L

E

E

G

N

N

V

V

E

E

L

V

P

P

L

A

I

V

Y

Y

R

A

R

G

V

V

P

T

A

P

A

A

R

D

R

R

R

K

E

Q

L

R

A

A

L

T

R

A

A

L

L

L

A

T

A

E

V

L

G

G

L

L

S

G

G

T

W

K

E

T

S

Q

H

N

T

R

P

P

A

S

E

Q

L

L

S

S

G

G

G

G

Q

Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

T

A

R

R

A

A

I

L

V

M

T

N

E

G

P

G

K

D

V

V

L

I

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

S

S

A

H

L

S

S

G

R

V

E

E

V

V

M

M

E

R

L

I

L

L

Q

R

S

E

F

L

N

N

R

-

S

A

R

A

G

G

L

H

T

T

I

I

V

I

M

L

V

V

T

T

H

H

E

D

A

M

D

Q

M

V

A

A

A

K

Y

N

A

A

E

T

R

R

R

I

I

I

L

E

F

I

R

S

D

D

G

G

R

E

I

I

A

I

S

S

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S54), G44 (= G55), S48 (= S59)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
41% identity, 93% coverage: 11:235/243 of query aligns to 1:225/233 of P75957

query
sites
P75957

M

M

L

N

E

K

P

I

I

L

I

L

R

Q

L

C

T

D

N

N

V

L

T

C

K

K

T

R

Y

Y

G

Q

S

E

G

G

S

S

A

V

A

Q

M

T

Q

D

A

V

L

L

K

H

G

N

I

V

D

S

L

F

A

S

I

V

H

G

P

E

G

G

E

E

F

M

V

M

A

A

V

I

M

V

G
|
G

P

S

S

S

G

G

S

S

G

G

K

K

S

S

T

T

C

L

M

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

T

T

P

P

S

T

S

S

G

G

V

D

F

V

L

I

F

F

D

N

G

G

V

Q

D

P

V

M

A

S

R

K

L

L

T

S

R

S

D

A

Q

A

R

K

A

A

L

E

L

L

R

R

R

N

H

Q

D

K

L

L

G

G

F

F

V

I

F

Y

Q

Q

G

F

F

H

N

H

L

L

L

L

S

P

R

D

T

F

S

T

A

A

L

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

I

L

Y

I

R

G

R

K

V

K

P

K

A

P

A

A

R

E

R

I

R

N

E

S

L

R

A

A

L

L

R

E

A

M

L

L

A

K

A

A

V

V

G

G

L

L

S

D

G

H

W

R

E

A

S

N

H

H

T

R

P

P

A

S

E

E

L

L

S

S

G

G

G

G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

T

A

R

R

A

A

I

L

V

V

T

N

E

N

P

P

K

R

V

L

L

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

A

A

R

L

N

S

A

R

D

E

S

V

I

M

F

E

Q

L

L

L

L

Q

G

S

E

F

L

N

N

R

R

S

L

R

Q

G

G

L

T

T

A

I

F

V

L

M

V

V

V

T

T

H

H

E

D

A

L

D

Q

M

L

A

A

A

K

Y

R

A

M

E

S

R

R

R

Q

I

L

L

E

F

M

R

R

D

D

G

G

R

R

I

L

A

T

S

A

G42 (= G52) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
42% identity, 91% coverage: 15:235/243 of query aligns to 2:222/226 of 7mdyC

query
sites
7mdyC

I

L

I

L

R

Q

L

C

T

D

N

N

V

L

T

C

K

K

T

R

Y
|
Y

G

Q

S

E

G

G

S

S

A

V

A

Q

M

T

Q

D

A

V

L

L

K

H

G

N

I

V

D

S

L

F

A

S

I

V

H

G

P

E

G

G

E

E

F

M

V

M

A

A

V

I

M

V

G

G

P

S

S

S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

T

T

P

P

S

T

S

S

G

G

V

D

F

V

L

I

F

F

D

N

G

G

V

Q

D

P

V

M

A

S

R

K

L

L

T

S

R

S

D

A

Q

A

R

K

A

A

L

E

L

L

R

R

R

N

H

Q

D

K

L

L

G

G

F

F

V

I

F

Y

Q
|
Q

G

F

F

H

N

H

L

L

L

L

S

P

R

D

T

F

S

T

A

A

L

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

I

L

Y

I

R

G

R

K

V

K

P

K

A

P

A

A

R

E

R

I

R

N

E

S

L

R

A

A

L

L

R

E

A

M

L

L

A

K

A

A

V

V

G

G

L

L

S

D

G

H

W

R

E

A

S

N

H
|
H

T

R

P

P

A

S

E
|
E

L

L

S
|
S

G
|
G

G
|
G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

T

A

R

R

A

A

I

L

V

V

T

N

E

N

P

P

K

R

V

L

L

V

L

L

A

A

D

D

E
|
E

P

P

T

T

G

G

N
|
N

L

L

D

D

S

A

A

R

L

N

S

A

R

D

E

S

V

I

M

F

E

Q

L

L

L

L

Q

G

S

E

F

L

N

N

R

R

S

L

R

Q

G

G

L

T

T

A

I

F

V

L

M

V

V

V

T

T

H
|
H

E

D

A

L

D

Q

M

L

A

A

A

K

Y

R

A

M

E

S

R

R

R

Q

I

L

L

E

F

M

R

R

D

D

G

G

R

R

I

L

A

T

S

A

binding adp orthovanadate: Y12 (= Y25), G42 (= G55), S43 (= S56), G44 (= G57), K45 (= K58), S46 (= S59), T47 (= T60), Q91 (= Q104), H138 (= H151), E142 (= E155), S144 (= S157), G145 (= G158), G146 (= G159), E168 (= E181), N172 (= N185), H201 (= H214)
binding magnesium ion: S46 (= S59), Q91 (= Q104)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
42% identity, 91% coverage: 15:235/243 of query aligns to 2:222/222 of 7arlD

query
sites
7arlD

I

L

I

L

R

Q

L

C

T

D

N

N

V

L

T

C

K

K

T

R

Y

Y

G

Q

S

E

G

G

S

S

A

V

A

Q

M

T

Q

D

A

V

L

L

K

H

G

N

I

V

D

S

L

F

A

S

I

V

H

G

P

E

G

G

E

E

F

M

V

M

A

A

V

I

M

V

G

G

P

S

S

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T

T

C

L

M

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

T

T

P

P

S

T

S

S

G

G

V

D

F

V

L

I

F

F

D

N

G

G

V

Q

D

P

V

M

A

S

R

K

L

L

T

S

R

S

D

A

Q

A

R

K

A

A

L

E

L

L

R

R

R

N

H

Q

D

K

L

L

G

G

F

F

V

I

F

Y

Q

Q

G

F

F

H

N

H

L

L

L

L

S

P

R

D

T

F

S

T

A

A

L

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

I

L

Y

I

R

G

R

K

V

K

P

K

A

P

A

A

R

E

R

I

R

N

E

S

L

R

A

A

L

L

R

E

A

M

L

L

A

K

A

A

V

V

G

G

L

L

S

D

G

H

W

R

E

A

S

N

H

H

T

R

P

P

A

S

E

E

L

L

S

S

G

G

G

G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

T

A

R

R

A

A

I

L

V

V

T

N

E

N

P

P

K

R

V

L

L

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

A

A

R

L

N

S

A

R

D

E

S

V

I

M

F

E

Q

L

L

L

L

Q

G

S

E

F

L

N

N

R

R

S

L

R

Q

G

G

L

T

T

A

I

F

V

L

M

V

V

V

T

T

H

H

E

D

A

L

D

Q

M

L

A

A

A

K

Y

R

A

M

E

S

R

R

R

Q

I

L

L

E

F

M

R

R

D

D

G

G

R

R

I

L

A

T

S

A

binding adenosine-5'-diphosphate: G42 (= G55), G44 (= G57), K45 (= K58), S46 (= S59)

9gvkD Cryo-em structure of endogenous atp-bound lolcde with lold-e171q mutations in nanodiscs (see paper)
41% identity, 91% coverage: 15:235/243 of query aligns to 3:223/226 of 9gvkD

query
sites
9gvkD

I

L

I

L

R

Q

L

C

T

D

N

N

V

L

T

C

K

K

T

R

Y
|
Y

G

Q

S

E

G

G

S

S

A

V

A

Q

M

T

Q

D

A

V

L

L

K

H

G

N

I

V

D

S

L

F

A

S

I

V

H

G

P

E

G

G

E

E

F

M

V

M

A

A

V

I

M

V

G

G

P

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

T

T

P

P

S

T

S

S

G

G

V

D

F

V

L

I

F

F

D

N

G

G

V

Q

D

P

V

M

A

S

R

K

L

L

T

S

R

S

D

A

Q

A

R

K

A

A

L

E

L

L

R

R

R

N

H

Q

D

K

L

L

G

G

F

F

V

I

F

Y

Q
|
Q

G

F

F

H

N

H

L

L

L

L

S

P

R

D

T

F

S

T

A

A

L

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

I

L

Y

I

R

G

R

K

V

K

P

K

A

P

A

A

R

E

R

I

R

N

E

S

L

R

A

A

L

L

R

E

A

M

L

L

A

K

A

A

V

V

G

G

L

L

S

D

G

H

W
x
R

E

A

S

N

H
|
H

T

R

P

P

A

S

E
|
E

L

L

S
|
S

G
|
G

G
|
G

Q
x
E

Q

R

Q

Q

R

R

V

V

A

A

I

I

T

A

R

R

A

A

I

L

V

V

T

N

E

N

P

P

K

R

V

L

L

V

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

N

L

L

D

D

S

A

A

R

L

N

S

A

R

D

E

S

V

I

M

F

E

Q

L

L

L

L

Q

G

S

E

F

L

N

N

R

R

S

L

R

Q

G

G

L

T

T

A

I

F

V

L

M

V

V

V

T

T

H

H

E

D

A

L

D

Q

M

L

A

A

A

K

Y

R

A

M

E

S

R

R

R

Q

I

L

L

E

F

M

R

R

D

D

G

G

R

R

I

L

A

T

S

A

binding adenosine-5'-triphosphate: Y13 (= Y25), S42 (= S54), G43 (= G55), G45 (= G57), K46 (= K58), S47 (= S59), T48 (= T60), Q92 (= Q104), R136 (≠ W148), H139 (= H151), E143 (= E155), S145 (= S157), G146 (= G158), G147 (= G159), E148 (≠ Q160)
binding magnesium ion: S47 (= S59), Q92 (= Q104)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
41% identity, 91% coverage: 15:235/243 of query aligns to 4:224/229 of 7v8iD

query
sites
7v8iD

I

L

I

L

R

Q

L

C

T

D

N

N

V

L

T

C

K

K

T

R

Y

Y

G

Q

S

E

G

G

S

S

A

V

A

Q

M

T

Q

D

A
x
V

L

L

K

H

G

N

I

V

D

S

L

F

A

S

I

V

H

G

P

E

G

G

E

E

F

M

V

M

A

A

V

I

M

V

G

G

P

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

L

N

H

I

L

L

L

G

G

C

G

L

L

D

D

T

T

P

P

S

T

S

S

G

G

V

D

F

V

L

I

F

F

D

N

G

G

V

Q

D

P

V

M

A

S

R

K

L

L

T

S

R

S

D

A

Q

A

R

K

A

A

L

E

L

L

R

R

R

N

H

Q

D

K

L

L

G

G

F

F

V

I

F

Y

Q
|
Q

G

F

F

H

N

H

L

L

L

L

S

P

R

D

T

F

S

T

A

A

L

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

I

L

Y

I

R

G

R

K

V

K

P

K

A

P

A

A

R

E

R

I

R

N

E

S

L

R

A

A

L

L

R

E

A

M

L

L

A

K

A

A

V

V

G

G

L

L

S

D

G

H

W
x
R

E

A

S

N

H
|
H

T

R

P

P

A

S

E
|
E

L

L

S
|
S

G

G

G
|
G

Q
x
E

Q

R

Q

Q

R

R

V

V

A

A

I

I

T

A

R

R

A

A

I

L

V

V

T

N

E

N

P

P

K

R

V

L

L

V

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

N

L

L

D

D

S

A

A

R

L

N

S

A

R

D

E

S

V

I

M

F

E

Q

L

L

L

L

Q

G

S

E

F

L

N

N

R

R

S

L

R

Q

G

G

L

T

T

A

I

F

V

L

M

V

V

V

T

T

H
|
H

E

D

A

L

D

Q

M

L

A

A

A

K

Y

R

A

M

E

S

R

R

R

Q

I

L

L

E

F

M

R

R

D

D

G

G

R

R

I

L

A

T

S

A

binding phosphoaminophosphonic acid-adenylate ester: V23 (≠ A34), S43 (= S54), G44 (= G55), G46 (= G57), K47 (= K58), S48 (= S59), T49 (= T60), Q93 (= Q104), R137 (≠ W148), H140 (= H151), E144 (= E155), S146 (= S157), G148 (= G159), E149 (≠ Q160), H203 (= H214)
binding magnesium ion: S48 (= S59), Q93 (= Q104)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
43% identity, 91% coverage: 15:234/243 of query aligns to 3:217/223 of 2pclA

query
sites
2pclA

I

I

I

L

R

R

L

A

T

E

N

N

V

I

T

K

K

K

T

V

Y

I

G

-

S

-

G

-

S

-

A

R

A

G

M

Y

Q

E

A

I

L

L

K

K

G

G

I

I

D

S

L

L

A

S

I

V

H

K

P

K

G

G

E

E

F

F

V

V

A

S

V

I

M

I

G

G

P

A

S

S

G

G

S

S

G

G

K

K

S

S

T

T

C

L

M

L

N

Y

I

I

L

L

G

G

C

L

L

L

D

D

T

A

P

P

S

T

S

E

G

G

V

K

F

V

L

F

F

L

D

E

G

G

V

K

D

E

V

V

A

D

R

Y

L

T

T

N

R

E

D

K

Q

E

R

L

A

S

L

L

L

L

R

R

R

N

H

R

D

K

L

L

G

G

F

F

V

V

F

F

Q

Q

G

F

F

H

N

Y

L

L

L

I

S

P

R

E

T

L

S

T

A

A

L

L

E

E

N

N

V

V

E

I

L

V

P

P

L

M

I

L

Y

K

R

M

R

G

V

K

P

P

A

K

A

K

R

E

R

A

R

K

E

E

L

R

A

G

L

E

R

Y

A

L

L

L

A

S

A

E

V

L

G

G

L

L

S

G

G

D

W

K

E

L

S

S

H

R

T

K

P

P

A

Y

E

E

L

L

S

S

G

G

G

G

Q

E

Q

Q

Q

Q

R

R

V

V

A

A

I

I

T

A

R

R

A

A

I

L

V

A

T

N

E

E

P

P

K

I

V

L

L

L

L

F

A

A

D

D

E
|
E

P
|
P

T
|
T

G

G

N

N

L

L

D

D

S

S

A

A

L

N

S

T

R

K

E

R

V

V

M

M

E

D

L

I

L

F

Q

L

S

K

F

I

N

N

R

E

S

G

R

-

G

G

L

T

T

S

I

I

V

V

M

M

V

V

T
|
T

H

H

E

E

A

R

D

E

M

L

A

A

A

E

Y

L

A

T

E

H

R

R

R

T

I

L

L

E

F

M

R

K

D

D

G

G

R

K

I

V

A

V

binding magnesium ion: E165 (= E181), P166 (= P182), T167 (= T183), T196 (= T213)

8g4cB Bceabs atpgs high res tm (see paper)
38% identity, 91% coverage: 15:235/243 of query aligns to 3:223/248 of 8g4cB

query
sites
8g4cB

I

I

I

L

R

E

L

A

T

N

N

K

V

I

T

R

K

K

T

S

Y
|
Y

G

G

S

N

G

K

S

L

A

N

A

K

M

Q

Q

E

A

V

L

L

K

K

G

G

I

I

D

D

L

I

A

H

I

I

H

E

P

K

G

G

E

E

F

F

V

V

A

S

V

I

M

M

G

G

P

A

S
|
S

G

G

S
|
S

G

G

K

K

S
x
T

T

T

C

L

M

L

N

N

I

V

L

L

G

S

C

S

L

I

D

D

T

Q

P

V

S

S

S

H

G

G

V

T

F

I

L

H

F

I

D

N

G

G

V

N

D

D

V

M

A

T

R

A

L

M

T

K

R

E

D

K

Q

Q

R

L

A

A

L

E

L

F

R

R

R

K

H

Q

D

H

L

L

G

G

F

F

V

I

F

F

Q

Q

G

D

F

Y

N

N

L

L

L

L

S

D

R

T

T

L

S

T

A

V

L

K

E

E

N

N

V

I

E

L

L

L

P

P

L

L

I

S

Y

I

R

T

R

K

V

L

P

S

-

K

-

K

-

E

A

A

A

N

R

R

R

K

R

F

E

E

L

E

A

V

L

A

R

K

A

E

L

L

A

-

A

-

V

-

G

G

L

I

S

Y

G

E

W

L

E

R

S

D

H

K

T

Y

P

P

A

N

E

E

L

I

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

T

A

S

I

A

T

G

R

R

A

A

I

F

V

I

T

H

E

D

P

P

K

S

V

I

L

I

L

F

A

A

D
|
D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

S

S

A

K

L

S

S

A

R

S

E

D

V

L

M

L

E

N

L

K

L

L

Q

S

S

Q

F

L

N

N

R

Q

S

K

R

R

G

N

L

A

T

T

I

I

V

I

M

M

V

V

T

T

H

H

E

D

A

P

D

V

M

A

A

A

A

S

Y

Y

A

C

E

G

R

R

R

V

I

I

L

F

F

I

R

K

D

D

G

G

R

Q

I

M

A

Y

S

T

binding phosphothiophosphoric acid-adenylate ester: Y13 (= Y25), S42 (= S54), S44 (= S56), T47 (≠ S59), D168 (= D180)

7tchB Bceab e169q variant atp-bound conformation (see paper)
38% identity, 91% coverage: 15:235/243 of query aligns to 2:222/245 of 7tchB

query
sites
7tchB

I

I

I

L

R

E

L

A

T

N

N

K

V

I

T

R

K

K

T

S

Y
|
Y

G

G

S

N

G

K

S

L

A

N

A

K

M
x
Q

Q

E

A
x
V

L

L

K

K

G

G

I

I

D

D

L

I

A

H

I

I

H

E

P

K

G

G

E

E

F

F

V

V

A

S

V

I

M

M

G

G

P

A

S
|
S

G
|
G

S
|
S

G

G

K
|
K

S
x
T

T

T

C

L

M

L

N

N

I

V

L

L

G

S

C

S

L

I

D

D

T

Q

P

V

S

S

S

H

G

G

V

T

F

I

L

H

F

I

D

N

G

G

V

N

D

D

V

M

A

T

R

A

L

M

T

K

R

E

D

K

Q

Q

R

L

A

A

L

E

L

F

R

R

R

K

H

Q

D

H

L

L

G

G

F

F

V

I

F

F

Q

Q

G

D

F

Y

N

N

L

L

L

L

S

D

R

T

T

L

S

T

A

V

L

K

E

E

N

N

V

I

E

L

L

L

P

P

L

L

I

S

Y

I

R

T

R

K

V

L

P

S

-

K

-

K

-

E

A

A

A

N

R

R

R

K

R

F

E

E

L

E

A

V

L

A

R

K

A

E

L

L

A

-

A

-

V

-

G

G

L

I

S

Y

G

E

W

L

E

R

S

D

H

K

T

Y

P

P

A

N

E
|
E

L

I

S

S

G
|
G

G
|
G

Q

Q

Q

K

Q

Q

R

R

V

T

A

S

I

A

T

G

R

R

A

A

I

F

V

I

T

H

E

D

P

P

K

S

V

I

L

I

L

F

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

S

S

A

K

L

S

S

A

R

S

E

D

V

L

M

L

E

N

L

K

L

L

Q

S

S

Q

F

L

N

N

R

Q

S

K

R

R

G

N

L

A

T

T

I

I

V

I

M

M

V

V

T

T

H

H

E

D

A

P

D

V

M

A

A

A

A

S

Y

Y

A

C

E

G

R

R

R

V

I

I

L

F

F

I

R

K

D

D

G

G

R

Q

I

M

A

Y

S

T

binding adenosine-5'-triphosphate: Y12 (= Y25), Q19 (≠ M32), V21 (≠ A34), S41 (= S54), G42 (= G55), S43 (= S56), K45 (= K58), T46 (≠ S59), E142 (= E155), G145 (= G158), G146 (= G159)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
41% identity, 84% coverage: 15:219/243 of query aligns to 1:204/343 of P30750

query
sites
P30750

I

M

I

I

R

K

L

L

T

S

N

N

V

I

T

T

K

K

T

V

Y

F

G

H

S

Q

G

G

S

T

A

R

A

T

M

I

Q

Q

A

A

L

L

K

N

G

N

I

V

D

S

L

L

A

H

I

V

H

P

P

A

G

G

E

Q

F

I

V

Y

A

G

V

V

M

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

I

N

R

I

C

L

V

G

N

C

L

L

L

D

E

T

R

P

P

S

T

S

E

G

G

V

S

F

V

L

L

F

V

D

D

G

G

V

Q

D

E

V

L

A

T

R

T

L

L

T

S

R

E

D

S

Q

E

R

L

A

T

L

K

L

A

R

R

R

R

H

Q

D

-

L

I

G

G

F

M

V

I

F

F

Q

Q

G

H

F

F

N

N

L

L

L

L

S

S

R

S

T

R

S

T

A

V

L

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

I

E

Y

L

R

D

R

N

V

T

P

P

A

K

A

D

R

E

R

V

R

K

E

R

L

R

A

V

L

T

R

E

A

L

L

L

A

S

A

L

V

V

G

G

L

L

S

G

G

D

W

K

E

H

S

D

H

S

T

Y

P

P

A

S

E

N

L

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

T

A

R

R

A

A

I

L

V

A

T

S

E

N

P

P

K

K

V

V

L

L

L

L

A

C

D

D

E
|
E

P

A

T

T

G

S

N

A

L

L

D

D

S

P

A

A

L

T

S

T

R

R

E

S

V

I

M

L

E

E

L

L

L

L

Q

K

S

D

F

I

N

N

R

R

S

R

R

L

G

G

L

L

T

T

I

I

V

L

M

L

V

I

T

T

H

H

E

E

A

M

D

D

M

V

A

V

40:46 (vs. 54:60, 86% identical) binding ATP
E166 (= E181) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
43% identity, 90% coverage: 14:231/243 of query aligns to 1:211/241 of 4u00A

query
sites
4u00A

P

P

I

I

I

I

R

R

L

I

T

R

N

N

V

L

T

H

K

K

T

W

Y
x
F

G

G

S

P

G

-

S

-

A

-

A

-

M

L

Q

H

A
x
V

L

L

K

K

G

G

I

I

D

H

L

L

A

E

I

V

H

A

P

P

G

G

E

E

F

K

V

L

A

V

V

I

M

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

I

N

R

I

T

L

I

G

N

C

R

L

L

D

E

T

D

P

F

S

Q

S

E

G

G

V

E

F

V

L

V

F

V

D

D

G

G

V

L

D

S

V

V

A

-

R

-

L

-

T

-

R

K

D

D

Q

D

R

R

A

A

L

L

L

R

R

E

-

I

R

R

H

R

D

E

L

V

G

G

F

M

V

V

F

F

Q

Q

G

Q

F

F

N

N

L

L

L

F

S

P

R

H

T

M

S

T

A

V

L

L

E

E

N

N

V

V

E

T

L

L

-

A

P

P

L

M

I

R

Y

V

R

R

R

R

V

W

P

P

A

R

A

E

R

K

R

A

R

E

E

K

L

K

A

A

L

L

R

E

A

L

L

L

A

E

A

R

V

V

G

G

L

I

S

L

G

D

W

Q

E

A

S

R

H

K

T

Y

P

P

A

A

E

Q

L

L

S

S

G

G

G

G

Q

Q

Q

Q

Q

Q

R

R

V

V

A

A

I

I

T

A

R

R

A

A

I

L

V

A

T

M

E

E

P

P

K

K

V

I

L

M

L

L

A

F

D

D

E

E

P

P

T

T

G

S

N

A

L

L

D

D

S

P

A

E

L

M

S

V

R

G

E

E

V

V

M

L

E

D

L

V

L

M

Q

R

S

D

F

L

N

A

R

Q

S

G

R

-

G

G

L

M

T

T

I

M

V

V

M

V

V

V

T

T

H

H

E

E

A

M

D

G

M

F

A

A

A

R

Y

E

A

V

E

A

R

D

R

R

I

V

L

V

F

F

R

M

D

D

G

G

binding adenosine-5'-diphosphate: F12 (≠ Y25), V17 (≠ A34), S37 (= S54), G38 (= G55), S39 (= S56), G40 (= G57), K41 (= K58), S42 (= S59), T43 (= T60)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
40% identity, 84% coverage: 15:219/243 of query aligns to 2:205/344 of 6cvlD

query
sites
6cvlD

I

M

I

I

R

K

L

L

T

S

N

N

V

I

T

T

K

K

T

V

Y
x
F

G

H

S
x
Q

G

G

S

T

A

R

A

T

M
x
I

Q

Q

A

A

L

L

K

N

G

N

I

V

D

S

L

L

A

H

I

V

H

P

P

A

G

G

E

Q

F

I

V

Y

A

G

V

V

M

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

I

N

R

I

C

L

V

G

N

C

L

L

L

D

E

T

R

P

P

S

T

S

E

G

G

V

S

F

V

L

L

F

V

D

D

G

G

V

Q

D

E

V

L

A

T

R

T

L

L

T

S

R

E

D

S

Q

E

R

L

A

T

L

K

L

A

R

R

R

R

H

Q

D

-

L

I

G

G

F

M

V

I

F

F

Q

Q

G

H

F

F

N

N

L

L

L

L

S

S

R

S

T

R

S

T

A

V

L

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

I

E

Y

L

R

D

R

N

V

T

P

P

A

K

A

D

R

E

R

V

R

K

E

R

L

R

A

V

L

T

R

E

A

L

L

L

A

S

A

L

V

V

G

G

L

L

S

G

G

D

W

K

E

H

S

D

H

S

T

Y

P

P

A

S

E
x
N

L

L

S
|
S

G

G

G

G

Q
|
Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

T

A

R

R

A

A

I

L

V

A

T

S

E

N

P

P

K

K

V

V

L

L

L

L

A

C

D

D

E

Q

P

A

T

T

G

S

N

A

L

L

D

D

S

P

A

A

L

T

S

T

R

R

E

S

V

I

M

L

E

E

L

L

L

L

Q

K

S

D

F

I

N

N

R

R

S

R

R

L

G

G

L

L

T

T

I

I

V

L

M

L

V

I

T

T

H
|
H

E

E

A

M

D

D

M

V

A

V

binding phosphothiophosphoric acid-adenylate ester: F12 (≠ Y25), Q14 (≠ S27), I19 (≠ M32), S41 (= S54), G42 (= G55), A43 (≠ S56), G44 (= G57), K45 (= K58), S46 (= S59), T47 (= T60), N141 (≠ E155), S143 (= S157), Q146 (= Q160), H200 (= H214)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
40% identity, 84% coverage: 15:219/243 of query aligns to 2:205/344 of 3tuzC

query
sites
3tuzC

I

M

I

I

R

K

L

L

T

S

N

N

V

I

T

T

K

K

T

V

Y
x
F

G

H

S
x
Q

G

G

S

T

A

R

A

T

M

I

Q

Q

A

A

L

L

K

N

G

N

I

V

D

S

L

L

A

H

I

V

H

P

P

A

G

G

E

Q

F

I

V

Y

A

G

V

V

M

I

G

G

P

A

S

S

G
|
G

S

A

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

I

N

R

I

C

L

V

G

N

C

L

L

L

D

E

T

R

P

P

S

T

S

E

G

G

V

S

F

V

L

L

F

V

D

D

G

G

V

Q

D

E

V

L

A

T

R

T

L

L

T

S

R

E

D

S

Q

E

R

L

A

T

L

K

L

A

R

R

R

R

H

Q

D

-

L

I

G

G

F

M

V

I

F

F

Q

Q

G

H

F

F

N

N

L

L

L

L

S

S

R

S

T

R

S

T

A

V

L

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

I

E

Y

L

R

D

R

N

V

T

P

P

A

K

A

D

R

E

R

V

R

K

E

R

L

R

A

V

L

T

R

E

A

L

L

L

A

S

A

L

V

V

G

G

L

L

S

G

G

D

W

K

E

H

S

D

H

S

T

Y

P

P

A

S

E

N

L

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

T

A

R

R

A

A

I

L

V

A

T

S

E

N

P

P

K

K

V

V

L

L

L

L

A

C

D

D

E

Q

P

A

T

T

G

S

N

A

L

L

D

D

S

P

A

A

L

T

S

T

R

R

E

S

V

I

M

L

E

E

L

L

L

L

Q

K

S

D

F

I

N

N

R

R

S

R

R

L

G

G

L

L

T

T

I

I

V

L

M

L

V

I

T

T

H

H

E

E

A

M

D

D

M

V

A

V

binding adenosine-5'-diphosphate: F12 (≠ Y25), Q14 (≠ S27), G42 (= G55), G44 (= G57), K45 (= K58), S46 (= S59), T47 (= T60)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
40% identity, 84% coverage: 15:219/243 of query aligns to 2:205/344 of 3tuiC

query
sites
3tuiC

I

M

I

I

R

K

L

L

T

S

N

N

V

I

T

T

K

K

T

V

Y
x
F

G

H

S

Q

G

G

S

T

A

R

A

T

M

I

Q

Q

A

A

L

L

K

N

G

N

I

V

D

S

L

L

A

H

I

V

H

P

P

A

G

G

E

Q

F

I

V

Y

A

G

V

V

M

I

G

G

P

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

I

N

R

I

C

L

V

G

N

C

L

L

L

D

E

T

R

P

P

S

T

S

E

G

G

V

S

F

V

L

L

F

V

D

D

G

G

V

Q

D

E

V

L

A

T

R

T

L

L

T

S

R

E

D

S

Q

E

R

L

A

T

L

K

L

A

R

R

R

R

H

Q

D

-

L

I

G

G

F

M

V

I

F

F

Q

Q

G

H

F

F

N

N

L

L

L

L

S

S

R

S

T

R

S

T

A

V

L

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

I

E

Y

L

R

D

R

N

V

T

P

P

A

K

A

D

R

E

R

V

R

K

E

R

L

R

A

V

L

T

R

E

A

L

L

L

A

S

A

L

V

V

G

G

L

L

S

G

G

D

W

K

E

H

S

D

H

S

T

Y

P

P

A

S

E

N

L

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

T

A

R

R

A

A

I

L

V

A

T

S

E

N

P

P

K

K

V

V

L

L

L

L

A

C

D

D

E

Q

P

A

T

T

G

S

N

A

L

L

D

D

S

P

A

A

L

T

S

T

R

R

E

S

V

I

M

L

E

E

L

L

L

L

Q

K

S

D

F

I

N

N

R

R

S

R

R

L

G

G

L

L

T

T

I

I

V

L

M

L

V

I

T

T

H

H

E

E

A

M

D

D

M

V

A

V

binding adenosine-5'-diphosphate: F12 (≠ Y25), G42 (= G55), A43 (≠ S56), G44 (= G57), K45 (= K58), S46 (= S59), T47 (= T60)

Query Sequence

>WP_244155490.1 NCBI__GCF_000422285.1:WP_244155490.1
MSRNAAAAETMLEPIIRLTNVTKTYGSGSAAMQALKGIDLAIHPGEFVAVMGPSGSGKST
CMNILGCLDTPSSGVFLFDGVDVARLTRDQRALLRRHDLGFVFQGFNLLSRTSALENVEL
PLIYRRVPAARRRELALRALAAVGLSGWESHTPAELSGGQQQRVAITRAIVTEPKVLLAD
EPTGNLDSALSREVMELLQSFNRSRGLTIVMVTHEADMAAYAERRILFRDGRIASIETER
NPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory