SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_268802025.1 NCBI__GCF_900167125.1:WP_268802025.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7kctA Crystal structure of the hydrogenobacter thermophilus 2-oxoglutarate carboxylase (ogc) biotin carboxylase (bc) domain dimer in complex with adenosine 5'-diphosphate magnesium salt (mgadp), adenosine 5'- diphosphate (adp, and bicarbonate anion (hydrogen carbonate/hco3-) (see paper)
36% identity, 94% coverage: 5:448/470 of query aligns to 6:418/453 of 7kctA

query
sites
7kctA

K

K

V

V

L

L

I

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

V

C

R

R

I

V

M

I

E

R

A

A

C

C

H

K

D

E

L

L

G

G

H

I

D

Q

F

T

V

V

A

A

V

I

Y

Y

T

N

A

E

E

I

D

E

Q

S

A

T

S

A

G

R

H

H

V

V

E

K

T

M

A

A

R

D

T

E

L

-

G

-

E

-

H

-

C

-

A

A

Y

Y

R

M

I

I

S

G

-

V

-

N

-

P

-

L

-

D

S

T

Y

Y

N

L

D

N

A

A

G

E

E

R

I

I

F

V

S

D

V

L

A

A

D

L

A

E

A

V

Q

G

A

A

T

E

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

F

L

S

A

E

E

N

N

Y

E

R

H

F

F

A

A

R

R

R

-

V

-

V

L

Q

C

R

E

Q

E

R

K

P

G

M

I

I

T

F

F

I

I

G

G

P

P

S

H

W

W

W

K

V

V

I

I

R

E

D

L

L

M

G

G

D
|
D

K
|
K

I

A

N
x
R

T

S

K

K

R

E

L

V

A

M

R

K

S

R

L

A

N

G

V

V

P

P

T

T

V

V

P

P

G

G

S

S

D

D

R

G

A

I

I

L

Y

K

D

D

E

V

L

E

E

E

A

A

E

K

E

R

I

I

A

A

E

K

N

E

L

-

F

-

D

-

F

-

Q

-

E

-

A

-

Q

-

G

-

I

I

S

G

C

Y

P

P

V

V

V
x
L

L

L

V

-

K
|
K

A

A

S

S

A

A

G

G

G
|
G

M

R

G

G

I
|
I

D

R

E

I

V

C

P

R

D

N

M

E

D

E

R

E

F

L

R

V

Q

R

I

N

Y

Y

R

E

R

N

I

A

R

Y

N

N

Y

E

S

A

M

V

R

K

Q

A

F

F

N

G

D

R

E

G

G

D

V

L

L

L

I

L

E
|
E

Q

K

R
x
Y

V
x
I

F

E

D

N

F

P

N

K

H
|
H

L

I

E

E

V

F

Q

Q

I

V

V

L

S

G

E

D

R

K

S

Y

G

G

Q

N

R

V

H

-

V

I

T

H

F

L

G

G

T

E

R

R

N

D

C

C

S

S

V

I

Q
|
Q

S

R

P

R

G

N

R

-

Q
|
Q

K
|
K

R

L

I

V

E

E

T

I

A

A

P

P

G

S

F

L

Y

L

P

L

D

T

G

P

I

E

T

Q

Y

R

S

E

F

Y

D

Y

A

G

Q

S

K

L

V

V

L

V

D

-

I

-

T

-

D

-

Y

-

S

-

L

-

R

K

M

A

A

A

E

K

A

E

I

I

K

G

Y

Y

D

Y

S

S

V

A

G

G

T

T

W

M

E
|
E

W

F

I
|
I

V

A

T

D

P

E

K

K

G

G

D

N

P

L

F

Y

L

F

L

I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q
|
Q

V
|
V

E
|
E

N

H

G

P

V

V

S

T

A

E

A

M

I

I

A

T

R

-

I

-

H

-

G

-

K

-

D

-

G

G

V

V

N

D

L

I

L

V

R

K

E

W

Q

Q

I

I

R

R

L

I

A

A

L

A

G

G

D

E

P

R

M

L

G

R

Y

Y

S

S

Q

Q

K

E

D

D

I

I

T

R

F

F

E

N

G

G

V

Y

S

S

I

I

E

E

Y

C

R
|
R

I

I

I

N

A

A

E

E

D

D

T

P

T

K

N

K

R

G

F

F

Q

A

P

P

W

S

V

I

G

G

Q

T

I

I

D

E

T

R

F

Y

Q

Y

W

V

K

P

S

G

A

G

P

-

W

-

L

F

T

G

M

I

H

R

T

V

H

E

V

H

P

A

T

S

D

S

R

K

A

G

Y

Y

Q

E

I

I

P

T

T

P

E

Y

Y

Y

D

D

P

S

N

L

L

I

A

A

L

K

A

L

I

I

V

V

W

W

G

A

A

P

D

L

L

W

E

E

E

V

A

A

K

V

A

D

R

R

G

M

M

R

V

S

A

A

L

L

D

E

G

T

V

Y

D

E

L

I

Q

S

G

G

N

V

D

K

T

T

active site: E276 (= E298), E289 (= E311), N291 (= N313), E297 (= E319), R339 (= R367)
binding adenosine-5'-diphosphate: K117 (= K119), L157 (≠ V168), K159 (= K171), G164 (= G176), G165 (= G177), G166 (= G178), I169 (= I181), E201 (= E213), Y203 (≠ R215), I204 (≠ V216), H209 (= H221), Q233 (= Q246), Q237 (= Q251), K238 (= K252), I278 (= I300), E289 (= E311), R293 (= R315), Q295 (= Q317), V296 (= V318), E297 (= E319), R339 (= R367)
binding bicarbonate ion: D116 (= D118), R119 (≠ N121)
binding magnesium ion: E276 (= E298), E289 (= E311)

8xl6A Structure of human 3-methylcrotonyl-coa carboxylase at apo-state (mcc- apo)
35% identity, 99% coverage: 5:470/470 of query aligns to 3:431/660 of 8xl6A

query
sites
8xl6A

K

K

V

V

L

L

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

V

C

R

R

I

V

M

M

E

R

A

T

C

A

H

K

D

K

L

L

G

G

H

V

D

Q

F

T

V

V

A

A

V

V

Y

Y

T

S

A

E

E

A

D

D

Q

R

A

N

S

S

G

M

H

H

V

V

E

D

T

M

A

A

R

D

T

E

L

A

G

Y

G

S

E

I

H

G

C

P

A

A

Y

P

R

S

I

Q

S

Q

S

S

Y

Y

N

L

D

S

A

M

G

E

E

K

I

I

F

I

S

Q

V

V

A

A

D

K

A

T

A

S

Q

A

A

A

T

Q

A

A

I

I

H

H

P

P

G

G

Y

C

G

G

F

F

F

L

S

S

E

E

N

N

Y

M

R

E

F

F

A

A

R

E

R

-

V

-

V

L

Q

C

R

K

Q

Q

R

E

P

G

M

I

I

I

F

F

I

I

G

G

P

P

S

P

W

P

W

S

V

A

I

I

R

R

D

D

L

M

G

G

D

I

K

K

I

S

N

T

T

S

K

K

R

S

L

I

A

M

R

A

S

A

L

A

N

G

V

V

P

P

T

V

V

V

P

E

G

G

S

-

D

-

R

-

A

-

I

Y

Y

H

D

G

E

E

L

D

E

Q

A

S

E

D

E

Q

I

C

A

L

E

K

N

-

L

-

F

-

D

-

F

-

Q

E

E

H

A

A

Q

R

G

R

I

I

S

G

C

Y

P

P

V

V

V

-

L

M

V

I

K

K

A

A

S

V

A

R

G

G

M

K

G

G

I

M

D

R

E

I

V

V

P

R

D

S

M

E

D

Q

R

E

F

F

R

Q

Q

E

I

Q

Y

L

R

E

R

S

I

A

R

R

N

R

Y

E

S

A

M

K

R

K

Q

S

F

F

N

N

D

D

E

D

G

A

V

M

L

L

I

I

E

E

Q

K

R

F

V

V

F

D

D

T

F

P

N

R

H

H

L

V

E

E

V

V

Q

Q

I

V

V

F

S

G

E

D

R

H

S

H

G

G

Q

N

R

A

H

V

V

Y

T

L

F

F

G

-

T

E

R

R

N

D

C

C

S

S

V

V

Q

Q

S

R

P

-

G

R

R

H

Q

Q

K

K

R

I

I

I

E

E

T

E

A

A

P

P

G

A

F

-

Y

-

P

-

D

P

G

G

I

I

T

K

Y

-

S

-

F

-

D

-

A

-

Q

S

K

E

V

V

L

R

D

K

I

L

T

G

D

E

Y

A

S

A

L

V

R

R

M

A

A

A

E

K

A

A

I

V

K

N

Y

Y

D

V

S

G

V

A

G

G

T

T

W

V

E

E

W

F

I

I

V

M

T

D

P

S

K

K

G

H

D

N

P

F

F

C

L

F

L

M

E

E

V

M

N

N

T

T

R

R

I

L

Q

Q

V

V

E

E

N

H

G

P

V

V

S

T

A

E

A

M

I

I

A

T

R

-

I

-

H

-

G

-

K

-

D

-

G

G

V

T

N

D

L

L

L

V

R

E

E

W

Q

Q

I

L

R

R

L

I

A

A

L

A

G

G

D

E

P

K

M

I

G

P

Y

L

S

S

Q

Q

K

E

D

E

I

I

T

T

F

L

E

Q

G

G

V

H

S

A

I

F

E

E

Y

A

R

R

I

I

I

Y

A

A

E

E

D

D

T

P

T

S

N

N

R

N

F

F

Q

M

P

P

W

V

V

A

G

G

Q

P

I

L

D

V

T

H

F

L

Q

-

W

-

K

-

S

S

A

T

P

P

W

R

L

A

T

D

M

P

H

S

T

T

H

R

V

I

P

E

T

T

D

G

-

V

-

R

R

Q

A

G

Y

D

Q

E

I

V

P

S

T

V

E

H

Y

Y

D

D

P

P

N

M

L

I

A

A

L

K

A

L

I

V

V

V

W

W

G

A

A

A

D

D

L

R

E

Q

E

A

A

A

K

L

A

T

R

K

G

L

M

R

V

Y

A

S

L

L

D

R

G

Q

V

Y

D

N

L

I

Q

V

G

G

N

-

D

-

T

-

A

-

G

-

L

-

E

-

L

L

K

H

S

T

N

N

L

I

D

D

F

F

L

L

R

-

T

L

N

N

N

L

K

S

G

G

L

H

L

P

E

E

F

F

Sites not aligning to the query:

binding biotin: 625, 626

2vpqB Crystal structure of biotin carboxylase from s. Aureus complexed with amppnp (see paper)
34% identity, 94% coverage: 5:448/470 of query aligns to 2:416/448 of 2vpqB

query
sites
2vpqB

K

K

V

V

L

L

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

V

V

R

R

I

I

M

I

E

R

A

A

C

C

H

R

D

D

L

L

G

G

H

I

D

Q

F

T

V

V

A

A

V

I

Y

Y

T

S

A

E

E

G

D

D

Q

K

A

D

S

A

G

L

H

H

V

T

E

Q

T

I

A

A

R

D

T

E

L

A

G

Y

G

C

E

V

H

G

C

P

A

T

Y

L

R

S

I

K

S

D

S

S

Y

Y

N

L

D

N

A

I

G

P

E

N

I

I

F

L

S

S

V

I

A

A

D

T

A

S

A

T

Q

G

A

C

T

D

A

G

I

V

H

H

P

P

G

G

Y

Y

G

G

F

F

F

L

S

A

E

E

N

N

Y

A

R

D

F

F

A

A

R

E

R

L

V

C

V

E

Q

A

R

C

Q

Q

R

-

P

-

M

L

I

K

F

F

I

I

G

G

P

P

S

S

W

Y

W

Q

V

S

I

I

R

Q

D

K

L

M

G

G

D

I

K
|
K

I

D

N
x
V

T

A

K

K

R

A

L

E

A

M

R

I

S

K

L

A

N

N

V

V

P

P

T

V

V

V

P

P

G

G

S

S

D

D

R

G

A

L

I

M

Y

K

D

D

E

V

L

S

E

E

A

A

E

K

I

I

A

A

E

K

N

K

L

-

F

-

D

-

F

-

Q

-

E

-

A

-

Q

-

G

-

I

I

S

G

C

Y

P

P

V

V

V
x
I

L

-

V

I

K
|
K

A

A

S

T

A

A

G

G

G
|
G

G

G

G
|
G

M

K

G

G

I
|
I

D

R

E

V

V

A

P

R

D

D

M

E

D

K

R

E

F

L

R

E

Q

T

I

G

Y

F

R

R

R

M

I

T

R

E

N

Q

Y

E

S

A

M

Q

R

T

Q

A

F

F

N

G

D

N

E

G

G

G

V

L

L

Y

I

M

E

E

Q

K

R
x
F

V
x
I

F

E

D

N

F

F

N

R

H
|
H

L

I

E

E

V

I

Q

Q

I

I

V

V

S

G

E

D

R

S

S

Y

G

G

Q

N

R

V

H

-

V

I

T

H

F

L

G

G

T

E

R

R

N

D

C

C

S

T

V

I

Q

Q

S

R

P

-

G
x
R

R

M

Q

Q

K

K

R

L

I

V

E

E

T

E

A

A

P

P

G

-

F

-

Y

-

P

-

D

-

G

-

I

-

T

-

Y

-

S

-

F

-

D

-

A

-

Q

S

K

P

V

I

L

L

D

D

I

E

T

T

-

R

-

E

-

M

-

G

D

N

Y

A

S

A

L

V

R

R

M

A

A

A

E

K

A

A

I

V

K

N

Y

Y

D

E

S

N

V

A

G

G

T
|
T

W

I

E
|
E

W

F

I
|
I

V

Y

T

D

P

L

K

N

G

D

D

N

P

K

F

F

-

Y

L

F

L
x
M

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V

V

E
|
E

N

H

G

P

V

V

S

T

A

E

A

M

I

V

A

T

R

-

I

-

H

-

G

-

K

-

D

-

G

G

V

I

N

D

L

L

L

V

R

K

E

L

Q

Q

I

L

R

Q

L

V

A

A

L

M

G

G

D

D

P

V

M

L

G

P

Y

Y

S

K

Q

Q

K

E

D

D

I

I

T

K

F

L

E

T

G

G

V

H

S

A

I

I

E

E

Y

F

R
|
R

I

I

I

N

A

A

E

E

D

N

T

P

T

Y

N

K

R

N

F

F

Q

M

P

P

W

S

V

P

G

G

Q

K

I

I

D

E

T

Q

F

Y

Q

L

W

A

K

P

S

G

A

G

P

Y

W

G

L

V

T

R

M

I

H

E

T

S

H

A

V

C

P

Y

T

T

D

N

R

-

A

-

Y

Y

Q

T

I

I

P

P

T

P

E

Y

Y

Y

D

D

P

S

N

M

L

V

A

A

L

K

A

L

I

I

V

I

W

H

G

E

A

P

D

T

L

R

E

D

E

E

A

A

K

I

A

M

R

A

G

G

M

I

V

R

A

A

L

L

D

S

G

E

V

F

D

V

L

V

Q

L

G

G

N

I

D

D

T

T

active site: V116 (≠ N121), K156 (= K171), H206 (= H221), R232 (≠ G249), T271 (= T296), E273 (= E298), E287 (= E311), N289 (= N313), R291 (= R315), E295 (= E319), R337 (= R367)
binding phosphoaminophosphonic acid-adenylate ester: K114 (= K119), I154 (≠ V168), K156 (= K171), G161 (= G176), G163 (= G178), I166 (= I181), F200 (≠ R215), I201 (≠ V216), E273 (= E298), I275 (= I300), M286 (≠ L310), E287 (= E311)
binding magnesium ion: E273 (= E298), E287 (= E311)

7ybuA Human propionyl-coenzyme a carboxylase (see paper)
34% identity, 94% coverage: 5:445/470 of query aligns to 7:419/670 of 7ybuA

query
sites
7ybuA

K

K

V

I

L

L

I

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

V

C

R

R

I

V

M

I

E

R

A

T

C

C

H

K

D

K

L

M

G

G

H

I

D

K

F

T

V

V

A

A

V

I

Y

H

T

S

A

D

E

V

D

D

Q

A

A

S

S

S

G

V

H

H

V

V

E

K

T

M

A

A

R

D

T

E

L

A

G

V

G

C

E

V

H

G

C

P

A

A

Y

P

R

T

I

S

S

K

S

S

Y

Y

N

L

D

N

A

M

G

D

E

A

I

I

F

M

S

E

V

A

A

I

D

K

A

K

A

T

Q

R

A

A

T

Q

A

A

I

V

H

H

P

P

G

G

Y

Y

G

G

F

F

F

L

S

S

E

E

N

N

Y

K

R

E

F

F

A

A

R

R

R

C

V

L

V

A

Q

A

R

E

Q

D

R

-

P

-

M

V

I

V

F

F

I

I

G

G

P

P

S

D

W

T

W

H

V

A

I

I

R

Q

D

A

L

M

G

G

D

D

K

K

I

I

N

E

T

S

K

K

R

L

L

L

A

A

R

K

S

K

L

A

N

E

V

V

P

N

T

T

V

I

P

P

G

G

S

F

D

D

R

G

A

V

I

V

Y

K

D

D

E

A

L

E

E

E

A

A

E

V

E

R

I

I

A

A

E

R

N

E

L

-

F

-

D

-

F

-

Q

-

E

-

A

-

Q

-

G

-

I

I

S

G

C

Y

P

P

V

V

V

-

L

M

V

I

K

K

A

A

S

S

A

A

G

G

M

K

G

G

I

M

D

R

E

I

V

A

P

W

D

D

M

D

D

E

R

E

F

T

R

R

Q

D

I

G

Y

F

R

R

R

L

I

S

R

S

N

Q

Y

E

S

A

M

A

R

S

Q

S

F

F

N

G

D

D

E

D

G

R

V

L

L

L

I

I

E

E

Q

K

R

F

V

I

F

D

D

N

F

P

N

R

H

H

L

I

E

E

V

I

Q

Q

I

V

V

L

S

G

E

D

R

K

S

H

G

G

Q

N

R

-

H

A

V

L

T

W

F

L

G

N

T

E

R

R

N

E

C

C

S

S

V

I

Q

Q

S

R

P

R

G

N

R

-

Q

Q

K

K

R

V

I

V

E

E

T

E

A

A

P

P

G

S

F

I

Y

F

P

L

D

D

G

A

I

E

T

T

Y

R

S

R

F

-

D

-

A

-

Q

-

K

-

V

-

L

-

D

-

D

A

I

M

T

G

D

E

Y

Q

S

A

L

V

R

A

M

L

A

A

E

R

A

A

I

V

K

K

Y

Y

D

S

S

S

V

A

G

G

T

T

W

V

E

E

W

F

I

L

V

V

T

D

P

S

K

K

G

K

D

N

P

F

F

Y

L

F

L

L

E

E

V

M

N

N

T

T

R

R

I

L

Q

Q

V

V

E

E

N

H

G

P

V

V

S

T

A

E

A

C

I

I

A

T

R

-

I

-

H

-

G

-

K

-

D

-

G

G

V

L

N

D

L

L

L

V

R

Q

E

E

Q

M

I

I

R

R

L

V

A

A

L

K

G

G

D

Y

P

P

M

L

G

R

Y

H

S

K

Q

Q

K

A

D

D

I

I

T

R

F

I

E

N

G

G

V

W

S

A

I

V

E

E

Y

C

R

R

I

V

I

Y

A

A

E

E

D

D

T

P

T

Y

N

K

R

S

F

F

-

G

Q

L

P

P

W

S

V

I

G

G

Q

R

I

L

D

S

T

Q

F

Y

Q

Q

W

E

K

P

S

-

A

-

P

-

W

-

L

-

T

-

M

-

H

-

T

L

H

H

V

L

P

P

-

G

-

V

-

R

T

V

D

D

R

S

A

G

Y

I

Q

Q

-

P

-

G

-

S

-

D

I

I

P

S

T

I

E

Y

Y

Y

D

D

P

P

N

M

L

I

A

S

L

K

A

L

I

I

V

T

W

Y

G

G

A

S

D

D

L

R

E

T

E

E

A

A

K

L

A

K

R

R

G

M

M

A

V

D

A

A

L

L

D

D

G

N

V

Y

D

V

L

I

Q

R

G

G

Sites not aligning to the query:

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: 635, 636

P9WPQ3 Biotin-dependent 3-methylcrotonyl-coenzyme A carboxylase alpha1 subunit; EC 6.3.4.14 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 97% coverage: 6:461/470 of query aligns to 5:425/654 of P9WPQ3

query
sites
P9WPQ3

V

V

L

L

I

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

V

V

R

R

I

V

M

I

E

R

A

T

C

L

H

R

D

R

L

L

G

G

H

I

D

R

F

S

V

V

A

A

V

V

Y

Y

T

S

A

D

E

P

D

D

Q

V

A

D

S

A

G

R

H

H

V

V

E

L

T

E

A

A

R

D

T

A

L

A

G

V

G

R

E

L

H

G

C

P

A

A

Y

P

R

A

I

R

S

E

S

S

Y

Y

N

L

D

D

A

I

G

G

E

K

I

V

F

L

S

D

V

A

A

A

D

A

A

R

A

T

Q

G

A

A

T

Q

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

F

L

S

A

E

E

N

N

Y

A

R

D

F

F

A

A

R

A

R

-

V

A

V

C

Q

E

R

R

Q

A

R

R

P

-

M

V

I

V

F

F

I

L

G

G

P

P

S

P

W

A

W

R

V

A

I

I

R

E

D

V

L

M

G

G

D

D

K

K

I

I

N

A

T

A

K

K

R

N

L

A

A

V

R

A

S

A

L

F

N

D

V

V

P

P

T

V

V

V

P

P

G

G

S

V

D

A

R

R

A

A

-

G

I

L

Y

T

D

D

E

D

L

A

E

L

A

V

E

T

E

A

I

A

A

A

E

E

N

-

L

-

F

-

D

-

F

-

Q

-

E

-

A

-

Q

-

G

-

I

V

S

G

C

Y

P

P

V

V

V

-

L

L

V

I

K

K

A

P

S

S

A

A

G

G

M

K

G

G

I

M

D

R

E

L

V

V

P

Q

D

D

M

P

D

A

R

R

F

L

R

P

Q

E

I

A

Y

L

R

V

R

S

I

A

R

R

N

R

Y

E

S

A

M

M

R

S

Q

S

F

F

N

G

D

D

E

D

G

T

V

L

L

F

I

L

E

E

Q

R

R

F

V

V

F

L

D

R

F

P

N

R

H

H

L

I

E

E

V

V

Q

Q

I

V

V

L

S

A

E

D

R

A

S

H

G

G

Q

N

R

-

H

V

V

V

T

H

F

L

G

G

T

E

R

R

N

E

C

C

S

S

V

L

Q

Q

S

R

P

-

G

R

R

H

Q

Q

K

K

R

V

I

I

E

E

T

E

A

A

P

P

G

S

F

P

Y

L

P

L

D

D

G

P

I

Q

T

T

Y

R

S

-

F

-

D

-

A

-

Q

-

K

-

V

-

L

-

D

E

D

R

I

I

T

G

D

V

Y

A

S

A

L

C

R

N

M

T

A

A

E

R

A

C

I

V

K

D

Y

Y

D

V

S

G

V

A

G

G

T

T

W

V

E

E

W

F

I

I

V

V

T

S

P

A

K

Q

-

R

-

P

G

D

D

E

P

F

F

F

L

F

L

M

E

E

V

M

N

N

T

T

R

R

I

L

Q

Q

V

V

E

E

N

H

G

P

V

V

S

T

A

E

A

A

I

I

A

T

R

-

I

-

H

-

G

-

K

-

D

-

G

G

V

L

N

D

L

L

L

V

R

E

E

W

Q

Q

I

L

R

R

L

V

A

G

L

A

G

G

D

E

P
x
K

M

L

G

G

Y

F

S

A

Q

Q

K

N

D

D

I

I

T

E

F

L

E

R

G

G

V

H

S

A

I

I

E

E

Y

A

R

R

I

V

I

Y

A

A

E

E

D

D

T

P

T

A

N

R

R

E

F

F

Q

L

P

P

W

T

V

G

G

G

Q

R

I

V

D

L

T

A

F

V

Q

F

W

E

K

P

S

A

A

G

P

P

W

G

L

V

T

R

M

V

H

D

T

S

H

S

V

L

P

L

T

G

D

-

R

-

A

G

Y

T

Q

V

I

V

P

G

T

S

E

D

Y

Y

D

D

P

P

N

L

L

L

A

T

L

K

A

V

I

I

V

A

W

H

G

G

A

A

D

D

L

R

E

E

A

-

K

-

A

-

R

-

G

-

M

-

V

-

A

A

L

L

D

D

G

R

V

L

D

D

L

Q

Q

A

G

L

N

A

D

R

T

T

A

A

G

V

L

L

E

G

L

V

K

Q

S

T

N

N

L

V

D

E

F

F

L

L

R

R

K322 (≠ P349) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8xl5A Structure of human propionyl-coa carboxylase in complex with propionyl-coa (pcc-pco)
34% identity, 94% coverage: 5:445/470 of query aligns to 5:417/668 of 8xl5A

query
sites
8xl5A

K

K

V

I

L

L

I

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

V

C

R

R

I

V

M

I

E

R

A

T

C

C

H

K

D

K

L

M

G

G

H

I

D

K

F

T

V

V

A

A

V

I

Y

H

T

S

A

D

E

V

D

D

Q

A

A

S

S

S

G

V

H

H

V

V

E

K

T

M

A

A

R

D

T

E

L

A

G

V

G

C

E

V

H

G

C

P

A

A

Y

P

R

T

I

S

S

K

S

S

Y

Y

N

L

D

N

A

M

G

D

E

A

I

I

F

M

S

E

V

A

A

I

D

K

A

K

A

T

Q

R

A

A

T

Q

A

A

I

V

H

H

P

P

G

G

Y

Y

G

G

F

F

F

L

S

S

E

E

N

N

Y

K

R

E

F

F

A

A

R

R

R

C

V

L

V

A

Q

A

R

E

Q

D

R

-

P

-

M

V

I

V

F

F

I

I

G

G

P

P

S

D

W

T

W

H

V

A

I

I

R

Q

D

A

L

M

G

G

D

D

K

K

I

I

N

E

T

S

K

K

R

L

L

L

A

A

R

K

S

K

L

A

N

E

V

V

P

N

T

T

V

I

P

P

G

G

S

F

D

D

R

G

A

V

I

V

Y

K

D

D

E

A

L

E

E

E

A

A

E

V

E

R

I

I

A

A

E

R

N

E

L

-

F

-

D

-

F

-

Q

-

E

-

A

-

Q

-

G

-

I

I

S

G

C

Y

P

P

V

V

V

-

L

M

V

I

K

K

A

A

S

S

A

A

G

G

M

K

G

G

I

M

D

R

E

I

V

A

P

W

D

D

M

D

D

E

R

E

F

T

R

R

Q

D

I

G

Y

F

R

R

R

L

I

S

R

S

N

Q

Y

E

S

A

M

A

R

S

Q

S

F

F

N

G

D

D

E

D

G

R

V

L

L

L

I

I

E

E

Q

K

R

F

V

I

F

D

D

N

F

P

N

R

H

H

L

I

E

E

V

I

Q

Q

I

V

V

L

S

G

E

D

R

K

S

H

G

G

Q

N

R

-

H

A

V

L

T

W

F

L

G

N

T

E

R

R

N

E

C

C

S

S

V

I

Q

Q

S

R

P

R

G

N

R

-

Q

Q

K

K

R

V

I

V

E

E

T

E

A

A

P

P

G

S

F

I

Y

F

P

L

D

D

G

A

I

E

T

T

Y

R

S

R

F

-

D

-

A

-

Q

-

K

-

V

-

L

-

D

-

D

A

I

M

T

G

D

E

Y

Q

S

A

L

V

R

A

M

L

A

A

E

R

A

A

I

V

K

K

Y

Y

D

S

S

S

V

A

G

G

T

T

W

V

E

E

W

F

I

L

V

V

T

D

P

S

K

K

G

K

D

N

P

F

F

Y

L

F

L

L

E

E

V

M

N

N

T

T

R

R

I

L

Q

Q

V

V

E

E

N

H

G

P

V

V

S

T

A

E

A

C

I

I

A

T

R

-

I

-

H

-

G

-

K

-

D

-

G

G

V

L

N

D

L

L

L

V

R

Q

E

E

Q

M

I

I

R

R

L

V

A

A

L

K

G

G

D

Y

P

P

M

L

G

R

Y

H

S

K

Q

Q

K

A

D

D

I

I

T

R

F

I

E

N

G

G

V

W

S

A

I

V

E

E

Y

C

R

R

I

V

I

Y

A

A

E

E

D

D

T

P

T

Y

N

K

R

S

F

F

-

G

Q

L

P

P

W

S

V

I

G

G

Q

R

I

L

D

S

T

Q

F

Y

Q

Q

W

E

K

P

S

-

A

-

P

-

W

-

L

-

T

-

M

-

H

-

T

L

H

H

V

L

P

P

-

G

-

V

-

R

T

V

D

D

R

S

A

G

Y

I

Q

Q

-

P

-

G

-

S

-

D

I

I

P

S

T

I

E

Y

Y

Y

D

D

P

P

N

M

L

I

A

S

L

K

A

L

I

I

V

T

W

Y

G

G

A

S

D

D

L

R

E

T

E

E

A

A

K

L

A

K

R

R

G

M

M

A

V

D

A

A

L

L

D

D

G

N

V

Y

D

V

L

I

Q

R

G

G

Sites not aligning to the query:

binding biotin: 633, 634

P05165 Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCase subunit alpha; Propanoyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.3 from Homo sapiens (Human) (see 6 papers)
34% identity, 94% coverage: 5:445/470 of query aligns to 65:477/728 of P05165

query
sites
P05165

K

K

V

I

L

L

I

V

A

A

N

N

R

R

G

G

E

E

I

I

A
|
A

V

C

R
|
R

I

V

M

I

E

R

A

T

C

C

H

K

D

K

L

M

G

G

H

I

D

K

F

T

V

V

A

A

V

I

Y

H

T

S

A

D

E

V

D

D

Q

A

A

S

S

S

G

V

H

H

V

V

E

K

T

M

A

A

R

D

T

E

L

A

G

V

G

C

E

V

H

G

C

P

A

A

Y

P

R

T

I

S

S

K

S

S

Y

Y

N

L

D

N

A

M

G

D

E

A

I

I

F

M

S

E

V

A

A

I

D

K

A

K

A

T

Q

R

A

A

T

Q

A
|
A

I

V

H

H

P

P

G

G

Y

Y

G

G

F

F

F

L

S

S

E

E

N

N

Y

K

R

E

F

F

A

A

R

R

R

C

V

L

V

A

Q

A

R

E

Q

D

R

-

P

-

M

V

I

V

F

F

I
|
I

G

G

P

P

S

D

W

T

W

H

V

A

I

I

R

Q

D

A

L

M

G

G

D

D

K

K

I

I

N

E

T

S

K

K

R

L

L

L

A

A

R

K

S

K

L

A

N

E

V

V

P

N

T

T

V

I

P

P

G

G

S

F

D

D

R
x
G

A

V

I

V

Y

K

D

D

E

A

L

E

E

E

A

A

E

V

E

R

I

I

A

A

E

R

N

E

L

-

F

-

D

-

F

-

Q

-

E

-

A

-

Q

-

G

-

I

I

S

G

C

Y

P

P

V

V

V

-

L

M

V

I

K

K

A

A

S

S

A

A

G

G

M

K

G

G

I
x
M

D

R

E

I

V

A

P

W

D

D

M

D

D

E

R

E

F

T

R

R

Q

D

I

G

Y

F

R

R

R

L

I

S

R

S

N

Q

Y

E

S

A

M

A

R

S

Q

S

F

F

N

G

D

D

E

D

G

R

V

L

L

L

I

I

E

E

Q

K

R

F

V

I

F

D

D

N

F

P

N

R

H

H

L

I

E

E

V

I

Q

Q

I

V

V

L

S

G

E

D

R

K

S

H

G

G

Q

N

R

-

H

A

V

L

T

W

F

L

G

N

T

E

R

R

N

E

C

C

S

S

V

I

Q

Q

S

R

P

R

G

N

R

-

Q
|
Q

K

K

R

V

I

V

E

E

T

E

A

A

P

P

G

S

F

I

Y

F

P

L

D

D

G

A

I

E

T

T

Y

R

S

R

F

-

D

-

A

-

Q

-

K

-

V

-

L

-

D

-

D

A

I

M

T

G

D

E

Y

Q

S

A

L

V

R

A

M

L

A

A

E

R

A

A

I

V

K

K

Y

Y

D

S

S

S

V

A

G

G

T

T

W

V

E

E

W

F

I

L

V

V

T

D

P

S

K

K

G

K

D

N

P

F

F

Y

L

F

L

L

E

E

V

M

N

N

T

T

R

R

I

L

Q

Q

V

V

E

E

N

H

G

P

V

V

S

T

A

E

A

C

I

I

A

T

R

-

I

-

H

-

G

-

K

-

D

-

G

G

V

L

N
x
D

L

L

L

V

R

Q

E

E

Q
x
M

I

I

R

R

L

V

A

A

L

K

G
|
G

D

Y

P

P

M

L

G

R

Y

H

S

K

Q

Q

K

A

D

D

I

I

T

R

F

I

E

N

G

G

V

W

S

A

I

V

E

E

Y
x
C

R
|
R

I

V

I

Y

A

A

E

E

D

D

T

P

T

Y

N

K

R

S

F

F

-

G

Q

L

P

P

W

S

V

I

G

G

Q

R

I

L

D

S

T

Q

F

Y

Q

Q

W

E

K
x
P

S

-

A

-

P

-

W

-

L

-

T

-

M

-

H

-

T

L

H

H

V

L

P

P

-

G

-

V

-

R

T

V

D

D

R

S

A

G

Y

I

Q

Q

-

P

-

G

-

S

-

D

I

I

P

S

T

I

E

Y

Y

Y

D

D

P

P

N

M

L

I

A

S

L

K

A

L

I

I

V

T

W

Y

G

G

A

S

D

D

L

R

E

T

E

E

A

A

K

L

A

K

R

R

G

M

M

A

V

D

A

A

L

L

D

D

G

N

V

Y

D

V

L

I

Q

R

G

G

A75 (= A15) to P: in PA-1; dbSNP:rs794727479
R77 (= R17) to W: in PA-1; loss of function; dbSNP:rs141371306
A138 (= A78) to T: in PA-1; loss of function; dbSNP:rs202247814
I164 (= I106) to T: in PA-1; loss of function; dbSNP:rs202247815
G197 (≠ R139) to E: in PA-1
M229 (≠ I181) to K: in PA-1; dbSNP:rs375628794
Q297 (= Q251) to R: in PA-1; dbSNP:rs2063109875
D368 (≠ N336) to G: in PA-1
M373 (≠ Q341) to K: in PA-1; unstable protein; loss of function; dbSNP:rs121964958
G379 (= G347) to V: in PA-1; dbSNP:rs794727087
C398 (≠ Y366) to R: in PA-1
R399 (= R367) to Q: in PA-1; dbSNP:rs1301904623
P423 (≠ K390) to L: in PA-1; dbSNP:rs1443858896

Sites not aligning to the query:

1:52 modified: transit peptide, Mitochondrion
532 natural variant: Missing (in PA-1)
551 V → F: in dbSNP:rs61749895
559 W → L: in PA-1; dbSNP:rs118169528
631 G → R: in PA-1; loss of function; dbSNP:rs796052018
668 G → R: in PA-1; loss of biotinylation; dbSNP:rs771438170
694 modified: N6-biotinyllysine; by HLCS
712 natural variant: Missing (in PA-1; loss of biotinylation)

4mv4A Crystal structure of biotin carboxylase from haemophilus influenzae in complex with amppcp and mg2 (see paper)
35% identity, 94% coverage: 5:448/470 of query aligns to 4:413/442 of 4mv4A

query
sites
4mv4A

K

K

V

V

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

V

L

R

R

I

I

M

L

E

R

A

A

C

C

H

K

D

E

L

L

G

G

H

I

D

K

F

T

V

V

A

A

V

V

Y

H

T

S

A

T

E

A

D

D

Q

R

A

D

S

L

G

K

H

H

V

V

E

L

T

L

A

A

R

D

T

E

L

T

G

I

G

C

E

I

H

G

C

P

A

A

Y

P

R

S

I

A

S

K

S

S

Y

Y

N

L

D

N

A

I

G

P

E

A

I

I

F

I

S

A

V

A

A

A

D

E

A

V

A

T

Q

G

A

A

T

D

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

F

L

S

S

E

E

N

N

Y

A

R

D

F

F

A

A

R

E

R

Q

V

V

V

-

Q

-

R

E

Q

R

R

S

P

G

M

F

I

T

F

F

I

I

G

G

P

P

S

T

W

A

W

D

V

V

I

I

R

R

D

L

L

M

G

G

D

D

K
|
K

I

V

N

S

T

A

K

I

R

K

L

A

A

M

R

K

S

K

L

A

N

G

V

V

P

P

T

C

V

V

P

P

G

G

S

S

D

D

R

G

A

P

I

V

Y

-

D

-

E

-

L

-

E

-

A

S

E

N

E

D

I

I

A

A

E

K

N

N

L

K

F

-

D

-

F

-

Q

E

E

I

A

A

Q

K

G

R

I

I

S

G

C

Y

P

P

V

I

L

-

V

I

K
|
K

A

A

S

S

A

-

G

G

G
|
G

G

G

M
|
M

G

R

I

V

D

-

E

-

V

V

P

R

D

S

M

E

D

D

R

A

F

L

R

E

Q

E

I

S

Y

I

R

A

R

M

I

T

R

K

N

A

Y

E

S

A

M

K

R

A

Q

A

F

F

N

N

D

N

E
x
D

G

M

V

V

L

Y

I

M

E
|
E

Q

K

R
x
Y

V
x
L

F

E

D

N

F

P

N

R

H
|
H

L

V

E

E

V

I

Q

Q

I

V

V

L

S

A

E

D

R

T

S

H

G

G

Q

N

R

A

H

-

V

V

T

Y

F

L

G

A

T

E

R

R

N

D

C

C

S

S

V

M

Q

Q

S

R

P

-

G
x
R

R
x
H

Q

Q

K

K

R

V

I

V

E

E

T

E

A

A

P

P

G

A

F

-

Y

-

P

-

D

P

G

G

I

I

T

T

Y

-

S

-

F

-

D

-

A

-

Q

E

K

E

V

V

L

R

D

R

D

D

I

I

T

G

D

S

Y

R

S

C

L

A

R

N

M

A

A

C

E

V

A

E

I

I

K

G

Y

Y

D

R

S

G

V

A

G

G

T
|
T

W

F

E
|
E

W

F

I
x
L

V

Y

T

E

P

-

K

N

G

G

D

E

P

F

F

Y

L

F

L

I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V

V

E
|
E

N

H

G

P

V

V

S

T

A

E

A

M

I

I

A

T

R

-

I

-

H

-

G

-

K

-

D

-

G

G

V

V

N

D

L

L

L

V

R

K

E

E

Q

Q

I

L

R

R

L

I

A

A

L

A

G

G

D

L

P

P

M

I

G

S

Y

F

S

K

Q

Q

K

E

D

D

I

I

T

K

F

V

E

K

G

G

V

H

S

A

I

M

E

E

Y

C

R
|
R

I

I

I

N

A

A

E

E

D

D

T

P

T

K

N

T

R

-

F

F

Q

L

P

P

W

S

V

P

G

G

Q

K

I

V

D

N

T

H

F

L

Q

H

W

S

K

P

S

G

A

G

P

L

W

G

L

V

T

R

M

W

H

D

T

S

H

H

V

V

P

Y

T

G

D

-

R

-

A

G

Y

Y

Q

T

I

V

P

P

T

P

E

H

Y

Y

D

D

P

S

N

M

L

I

A

A

L

K

A

L

I

I

V

T

W

Y

G

G

A

D

D

T

L

R

E

E

E

V

A

A

K

I

A

R

R

R

G

M

M

Q

V

N

A

A

L

L

D

S

G

E

V

T

D

I

L

I

Q

D

G

G

N

I

D

K

T

T

active site: K116 (= K119), K159 (= K171), D193 (≠ E208), H206 (= H221), R232 (≠ G249), T271 (= T296), E273 (= E298), E285 (= E311), N287 (= N313), R289 (= R315), E293 (= E319), R335 (= R367)
binding phosphomethylphosphonic acid adenylate ester: K159 (= K171), G164 (= G177), M166 (= M179), E198 (= E213), Y200 (≠ R215), L201 (≠ V216), H233 (≠ R250), L275 (≠ I300), E285 (= E311)
binding magnesium ion: E273 (= E298), E285 (= E311)

2vr1A Crystal structure of biotin carboxylase from e. Coli in complex with atp analog, adpcf2p. (see paper)
33% identity, 97% coverage: 5:459/470 of query aligns to 4:418/444 of 2vr1A

query
sites
2vr1A

K

K

V

I

L

V

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

V

L

R

R

I

I

M

L

E

R

A

A

C

C

H

K

D

E

L

L

G

G

H

I

D

K

F

T

V

V

A

A

V

V

Y

H

T

S

A

S

E

A

D

D

Q

R

A

D

S

L

G

K

H

H

V

V

E

L

T

L

A

A

R

D

T

E

L

T

G

V

G

C

E

I

H

G

C

P

A

A

Y

P

R

S

I

V

S

K

S

S

Y

Y

N

L

D

N

A

I

G

P

E

A

I

I

F

I

S

S

V

A

A

A

D

E

A

I

A

T

Q

G

A

A

T

V

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

F

L

S

S

E

E

N

N

Y

A

R

N

F

F

A

A

R

E

R

Q

V

V

V

-

Q

-

R

E

Q

R

R

S

P

G

M

F

I

I

F

F

I

I

G

G

P

P

S

K

W

A

W

E

V

T

I

I

R

R

D

L

L

M

G

G

D

D

K
|
K

I

V

N

S

T

A

K

I

R

A

L

A

A

M

R

K

S

K

L

A

N

G

V

V

P

P

T

C

V

V

P

P

G

G

S

S

D

D

R

G

A

P

I

L

Y

G

D

D

E

D

L

M

E

D

A

K

E

N

E

R

I

A

A

I

E

-

N

-

L

-

F

-

D

-

F

-

Q

-

E

-

A

A

Q

K

G

R

I

I

S

G

C

Y

P

P

V

V

V

I

L

-

V

I

K
|
K

A

A

S

S

A

G

G

G

G
x
R

G

G

M
|
M

G

R

I

V

D

-

E

-

V

V

P

R

D

G

M

D

D

A

R

E

F

L

R

A

Q

Q

I

S

Y

I

R

S

R

M

I

T

R

R

N

A

Y

E

S

A

M

K

R

A

Q

A

F

F

N

S

D

N

E
x
D

G

M

V

V

L

Y

I

M

E

E

Q

K

R
x
Y

V
x
L

F

E

D

N

F

P

N

R

H
|
H

L

V

E

E

V

I

Q

Q

I

V

V

L

S

A

E

D

R

G

S

Q

G

G

Q

N

R

A

H

-

V

I

T

Y

F

L

G

A

T

E

R

R

N

D

C

C

S

S

V

M

Q

Q

S

R

P
x
R

G

-

R

H

Q

Q

K

K

R

V

I

V

E

E

-

E

-

A

T

P

A

A

P

P

G

G

F

I

Y

T

P

P

D

E

G

L

I

R

T

R

Y

Y

S

-

F

-

D

-

A

-

Q

-

K

-

V

-

L

-

D

-

I

I

T

G

D

E

Y

R

S

C

L

A

R

K

M

A

A

C

E

V

A

D

I

I

K

G

Y

Y

D

R

S

G

V

A

G

G

T
|
T

W

F

E
|
E

W

F

I
x
L

V

F

T

E

P

-

K

N

G

G

D

E

P

F

F

Y

L

F

L

I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

I

Q

Q

V

V

E
|
E

N

H

G

P

V

V

S

T

A

E

A

M

I

I

A

T

R

-

I

-

H

-

G

-

K

-

D

-

G

G

V

V

N

D

L

L

L

I

R

K

E

E

Q

Q

I

L

R

R

L

I

A

A

L

A

G

G

D

Q

P

P

M

L

G

S

Y

I

S

K

Q

Q

K

E

D

E

I

V

T

H

F

V

E

R

G

G

V

H

S

A

I

V

E

E

Y

C

R
|
R

I

I

I

N

A

A

E

E

D

D

T

-

T

P

N

N

R

T

F

F

Q

L

P

P

W

S

V

P

G

G

Q

K

I

I

D

T

T

R

F

F

Q

H

W

A

K

P

S

G

A

G

P

F

W

G

L

V

T

R

M

W

H

E

T

S

H

H

V

I

P

Y

T

A

D

-

R

-

A

G

Y

Y

Q

T

I

V

P

P

T

P

E

Y

Y

Y

D

D

P

S

N

M

L

I

A

G

L

K

A

L

I

I

V

C

W

Y

G

G

A

E

D

N

L

R

E

D

E

V

A

A

K

I

A

A

R

R

G

M

M

K

V

N

A

A

L

L

D

Q

G

E

V

L

D

I

L

I

Q

D

G

G

N

-

D

-

T

-

A

-

G

-

L

-

E

-

L

I

K

K

S

T

N

N

L

V

D

D

F

L

active site: K116 (= K119), K159 (= K171), D194 (≠ E208), H207 (= H221), R233 (≠ P248), T272 (= T296), E274 (= E298), E286 (= E311), N288 (= N313), R290 (= R315), E294 (= E319), R336 (= R367)
binding phosphodifluoromethylphosphonic acid-adenylate ester: K159 (= K171), R165 (≠ G177), M167 (= M179), Y201 (≠ R215), L202 (≠ V216), E274 (= E298), L276 (≠ I300), E286 (= E311), N288 (= N313)

Sites not aligning to the query:

binding phosphodifluoromethylphosphonic acid-adenylate ester: 435

2vqdA Crystal structure of biotin carboxylase from pseudomonas aeruginosa complexed with ampcp (see paper)
34% identity, 96% coverage: 5:457/470 of query aligns to 4:424/447 of 2vqdA

query
sites
2vqdA

K

K

V

V

L

L

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

V

L

R

R

I

I

M

L

E

R

A

A

C

C

H

K

D

E

L

L

G

G

H

I

D

K

F

T

V

V

A

A

V

V

Y

H

T

S

A

T

E

A

D

D

Q

R

A

E

S

L

G

M

H

H

V

L

E

S

T

L

A

A

R

D

T

E

L

S

G

V

G

C

E

I

H

G

C

P

A

A

Y

P

R

A

I

T

S

Q

S

S

Y

Y

N

L

D

Q

A

I

G

P

E

A

I

I

F

I

S

A

V

A

A

A

D

E

A

V

A

T

Q

G

A

A

T

T

A

A

I

I

H

H

P

P

G

G

Y

Y

G

G

F

F

F

L

S

A

E

E

N

N

Y

A

R

D

F

F

A

A

R

E

R

Q

V

I

V

-

Q

-

R

E

Q

R

R

S

P

G

M

F

I

T

F

F

I

V

G

G

P

P

S

T

W

A

W

E

V

V

I

I

R

R

D

L

L

M

G

G

D

D

K
|
K

I

V

N

S

T

A

K

K

R

D

L

A

A

M

R

K

S

R

L

A

N

G

V

V

P

P

T

T

V

V

P

P

G

G

S

S

D

D

R

G

A

P

I

L

Y

P

D

E

E

D

L

-

E

-

A

-

E

E

I

T

A

A

E

L

N

A

L

I

F

-

D

-

F

-

Q

-

E

-

A

A

Q

R

G

E

I

V

S

G

C

Y

P

P

V

V

V
x
I

L

-

V

I

K
|
K

A

A

S

A

A

G

G

G

G
|
G

G

G

G
|
G

M

R

G

G

I

M

D

R

E

V

V

V

P

Y

D

D

M

E

D

S

R

E

F

L

R

I

Q

K

I

S

Y

A

R

K

R

L

I

T

R

R

N

T

Y

E

S

A

M

G

R

A

Q

A

F

F

N

G

D

N

E
x
P

G

M

V

V

L

Y

I

L

E

E

Q

K

R
x
F

V
x
L

F

T

D

N

F

P

N

R

H
|
H

L

V

E

E

V

V

Q

Q

I

V

V

L

S

S

E

D

R

G

S

Q

G

G

Q

N

R

A

H

-

V

I

T

H

F

L

G

G

T

D

R

R

N

D

C

C

S

S

V

L

Q
|
Q

S

R

P

-

G
x
R

R
x
H

Q

Q

K

K

R

V

I

I

E

E

T

E

A

A

P

P

G

A

F

P

Y

G

P

I

D

D

G

-

I

-

T

-

Y

-

S

-

F

-

D

-

A

-

Q

E

K

K

V

A

L

R

D

Q

D

E

I

V

T

F

D

A

Y

R

S

C

L

V

R

Q

M

A

A

C

E

I

A

E

I

I

K

G

Y

Y

D

R

S

G

V

A

G

G

T
|
T

W

F

E
|
E

W

F

I
x
L

V

Y

T

E

P

-

K

N

G

G

D

R

P

F

F

Y

L

F

L

I

E
|
E

V

M

N
|
N

T

T

R
|
R

I

V

Q

Q

V

V

E
|
E

N

H

G

P

V

V

S

S

A

E

A

M

I

V

A

T

R

-

I

-

H

-

G

-

K

-

D

-

G

G

V

V

N

D

L

I

L

V

R

K

E

E

Q

M

I

L

R

R

L

I

A

A

L

S

G

G

D

E

P

K

M

L

G

S

Y

I

S

R

Q

Q

K

E

D

D

I

V

T

V

F

I

E

R

G

G

V

H

S

A

I

L

E

E

Y

C

R
|
R

I

I

I

N

A

A

E

E

D

D

T

P

T

K

N

T

R

-

F

F

Q

M

P

P

W

S

V

P

G

G

Q

K

I

V

D

K

T

H

F

F

Q

H

W

A

K

P

S

G

A

G

P

N

W

G

L

V

T

R

M

V

H

D

T

S

H

H

V

L

P

Y

T

S

D

-

R

-

A

G

Y

Y

Q

S

I

V

P

P

T

P

E

N

Y

Y

D

D

P

S

N

L

L

V

A

G

L

K

A

V

I

I

V

T

W

Y

G

G

A

A

D

D

L

R

E

D

E

E

A

A

K

L

A

A

R

R

G

M

M

R

V

N

A

A

L

L

D

D

G

E

V

L

D

I

L

V

Q

D

G

G

N

I

D

K

T

T

A

-

G

N

L

T

E

E

L

L

K

H

S

K

N

D

L

L

active site: K116 (= K119), K159 (= K171), P196 (≠ E208), H209 (= H221), R235 (≠ G249), T274 (= T296), E276 (= E298), E288 (= E311), N290 (= N313), R292 (= R315), E296 (= E319), R338 (= R367)
binding phosphomethylphosphonic acid adenosyl ester: K116 (= K119), I157 (≠ V168), K159 (= K171), G164 (= G176), G166 (= G178), F203 (≠ R215), L204 (≠ V216), H209 (= H221), Q233 (= Q246), H236 (≠ R250), L278 (≠ I300), E288 (= E311)
binding magnesium ion: E276 (= E298), E288 (= E311)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenosyl ester: 437

8sgxX Leishmania tarentolae propionyl-coa carboxylase (alpha-4-beta-6) (see paper)
32% identity, 97% coverage: 6:461/470 of query aligns to 1:419/657 of 8sgxX

query
sites
8sgxX

V

V

L

L

I

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

V

C

R

R

I

V

M

M

E

A

A

T

C

C

H

R

D

R

L

L

G

G

H

I

D

K

F

T

V

V

A

A

V

V

Y

Y

T

S

A

T

E

A

D

D

Q

E

A

Q

S

A

G

K

H

H

V

V

E

K

T

V

A

A

R

D

T

E

L

S

G

V

G

C

E

I

H

G

C

P

A

P

Y

A

R

S

I

V

S

E

S

S

Y

Y

N

L

D

C

A

I

G

D

E

K

I

I

F

V

S

D

V

A

A

C

D

K

A

K

A

T

Q

G