SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_280115907.1 NCBI__GCF_000009265.1:WP_280115907.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
29% identity, 91% coverage: 22:431/450 of query aligns to 9:425/425 of O59010

query
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S65 (≠ T76) mutation to V: Strongly decreased chloride conductance.
R276 (≠ T287) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ TST 287:289) binding L-aspartate
M311 (≠ L322) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (= T325) binding L-aspartate
V355 (≠ I366) binding L-aspartate
D394 (≠ A400) binding L-aspartate
M395 (≠ E401) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (≠ L403) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N407) binding L-aspartate
D405 (≠ N411) mutation to N: Strongly decreased affinity for aspartate.

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
29% identity, 89% coverage: 22:423/450 of query aligns to 1:409/409 of 6bavA

query
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binding benzylcysteine: R268 (≠ T287), S270 (≠ T289), T306 (= T325), Y309 (= Y328), D382 (≠ H396), D386 (≠ A400), C389 (≠ L403), T390 (= T404)

2nwwA Crystal structure of gltph in complex with tboa (see paper)
29% identity, 89% coverage: 23:422/450 of query aligns to 1:407/407 of 2nwwA

query
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G

N

D

A

L

L

T

A

G

T

T

V

A

V

I

V

V

A

A

K

K

W

T

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (≠ T289), T305 (= T325), A349 (= A369), G350 (≠ A370), D385 (≠ A400), R388 (≠ L403), T389 (= T404), N392 (= N407)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
29% identity, 89% coverage: 22:422/450 of query aligns to 1:408/408 of 6bauA

query
sites
6bauA

R

E

F

Y

G

P

L

V

F

L

G

Q

Q

K

L

I

W

L

F

I

Q

G

V

L

L

I

A

L

G

G

T

A

L

I

L

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

A

G

P

Y

A

A

T

D

A

A

V

V

A

K

-

T

-

Y

M

V

K

K

P

P

F

F

G

G

D

D

A

L

F

F

I

V

K

R

M

L

I

L

R

K

M

M

M

L

I

V

G

M

P

P

I

I

I

V

F

F

V

A

T

S

V

L

V

V

H

V

G

G

I

A

A

A

G

S

M

I

N

S

D

P

M

A

K

R

S

-

V

L

G

G

R

R

V

V

A

G

L

V

K

K

S

I

I

V

Y

Y

F

Y

E

L

A

L

I

T

T

S

I

A

L

F

A

A

L

V

I

T

F

L

G

G

L

I

I

I

A

M

V

A

D

R

L

L

W

F

R

N

P

P

G

G

E

A

G

G

M

I

N

H

I

L

D

-

A

-

T

-

A

A

I

V

D

G

T

G

A

Q

S

Q

I

F

K

Q

T

P

Y

K

L

Q

A

A

T

-

A

-

H

-

D

-

Q

P

S

P

I

L

S

V

S

K

Y

I

F

L

L

L

D

D

I

I

I

V

P

P

D

T

T

N

F

P

A

F

S

G

A

A

L

L

T

A

E

N

G

G

H

Q

V

V

L

L

Q

P

V

T

L

I

F

F

I

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

A

T

A

Y

I

L

-

M

-

N

-

S

-

E

G

N

E

E

R

K

G

V

R

R

P

K

-

S

-

A

-

E

-

T

V

L

F

L

Q

D

V

A

I

I

E

N

S

G

A

L

S

A

Q

E

G

A

F

M

F

Y

K

K

I

I

I

V

G

N

Y

G

I

V

M

M

Y

Q

F

Y

A

A

P

P

L

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

V

M

G

A

Q

E

F

Q

G

G

T

V

A

K

S

V

L

V

W

G

S

E

L

L

G

A

E

K

L

V

I

T

I

A

E

A

F

V

F

Y

V

V

V

G

C

L

A

T

L

L

F

Q

T

I

T

L

I

L

V

V

L

Y

G

F

S

V

V

L

A

L

H

K

W

I

C

Y

G

G

V

I

S

D

L

P

W

I

R

S

L

F

L

I

G

K

Y

K

L

A

W

K

D

D

E

A

I

M

V

L

I

T

V

A

A

F

A

V

T

T

T

R

S

S

T
x
S

E

S

T

G

V

T

L

L

P

P

R

V

L

T

I

M

Q

R

K

V

M

A

R

K

K

E

A

M

G

G

C

I

E

S

E

E

S

G

V

I

V

Y

G

S

F

F

V

T

I

L

P

P

A

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

C

A

L

L

Y

Y

L

Q

T

G

T

V

V

C

A

T

V

F

F

F

L

I

A

A

Q

N

A

A

T

L

N

G

T

S

H

H

L

L

T

T

F

V

W

G

H

Q

E

Q

L

L

G

T

L

I

I

V

A

L

V

T

L

A

L

V

L

L

T

A

S

S

K

I

G

G

A

T

A
|
A

G
|
G

I
x
V

A

P

G

G

A

A

A
x
G

F

A

V

I

V

M

L

L

A

A

A

M

T

V

L

L

N

E

T

S

T

V

G

G

-

L

-

P

-

L

T

T

I

D

P

P

-

N

-

V

V

A

A

A

S

A

I

Y

A

A

L

M

I

I

L

L

G

G

I

I

H

D

R

A

I

I

L

L

A
x
D

E

M

G

G

L
x
C

T
|
T

F

M

V

V

N
|
N

L

V

I

T

G

G

N

D

A

L

L

T

A

G

T

T

V

A

V

I

V

V

A

A

K

K

W

T

E

E

binding cysteine: S270 (≠ T289), M303 (≠ L322), T306 (= T325), A345 (= A364), G346 (= G365), V347 (≠ I366), G351 (≠ A370), D386 (≠ A400), C389 (≠ L403), T390 (= T404), N393 (= N407)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
29% identity, 90% coverage: 22:425/450 of query aligns to 9:419/419 of 6x15A

query
sites
6x15A

R

E

F

Y

G

P

L

V

F

L

G

Q

Q

K

L

I

W

L

F

I

Q

G

V

L

L

I

A

L

G

G

T

A

L

I

L

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

A

G

P

Y

A

A

T

H

A

A

V

V

A

H

-

T

-

Y

M

V

K

K

P

P

F
|
F

G

G

D

D

A

L

F

F

I

V

K

R

M

L

I

L

R

C

M

M

M

L

I

V

G

M

P

P

I

I

I

V

F

F

V

A

T

S

V

L

V

V

H

V

G

G

I

A

A

A

G

S

M

I

N

S

D
x
P

M

A

K

R

S

-

V

L

G

G

R

R

V

V

A

G

L

V

K

K

S

I

I

V

Y

Y

F
x
Y

E

L

A
x
L

I
x
T

T
x
S

I

A

L
x
F

A

A

L

V

I

T

F

L

G

G

L

I

I

I

A

M

V

A

D

R

L

L

W

F

R

N

P

P

G

G

E

A

G

G

M

I

N

H

I

L

D

-

A

-

T

-

A

A

I

V

D

G

T

G

A

Q

S

Q

I

F

K

Q

T

P

Y

H

L

Q

A

A

T

-

A

-

H

-

D

-

Q

P

S

P

I
x
L

S

V

S

H

Y
x
I

F

L

L

L

D

D

I

I

I

V

P

P

D

T

T

N

F

P

A

F

S

G

A

A

L

L

T

A

E

N

G

G

H

Q

V

V

L

L

Q

P

V

T

L

I

F

F

I

F

S

A

V
x
I

L

I

F

L

G

G

I

I

A

A

I

I

A

T

A
x
Y

I

L

-

M

-

N

-

S

-

E

G

N

E

E

R

K

G

V

R

R

P

K

-

S

-

A

-

E

-

T

V

L

F

L

Q

D

V

A

I

I

E

N

S

G

A

L

S

A

Q

E

G

A

F

M

F

Y

K
|
K

I

I

I

V

G

N

Y
x
G

I

V

M

M

Y

Q

F

Y

A

A

P

P

L
x
I

G

G

A

V

F
|
F

G

A

A

L

I

I

A

A

F

Y

T

V

V

M

G

A

Q

E

F

Q

G

G

T

V

A

H

S

V

L

V

W

G

S

E

L

L

G

A

E

K

L

V

I

T

I

A

E

A

F

V

F

Y

V

V

V

G

C

L

A

T

L

L

F

Q

T

I

T

L

I

L

V

V

L

Y

G

F

S

V

V
x
L

A

L

H

K

W

I

C

Y

G

G

V

I

S

D

L

P

W

I

R

S

L

F

L
x
I

G

K

Y

H

L

A

W

K

D

D

E

A

I
x
M

V

L

I

T

V

A

A

F

A

V

T

T

T

R

S
|
S

T
x
S

E

S

T

G

V

T

L

L

P

P

R

V

L

T

I

M

Q

R

K

V

M

A

R

K

K

E

A

M

G

G

C

I

E

S

E

E

S

G

V

I

V

Y

G

S

F

F

V

T

I

L

P

P

A

L

G
|
G

Y

A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

T
|
T

C

A

L

L

Y

Y

L

Q

T

G

T

V

V

A

A

T

V

F

F

F

L

I

A

A

Q

N

A

A

T

L

N

G

T

S

H

H

L

L

T
|
T

F
x
V

W
x
G

H

Q

E

Q

L

L

G
x
T

L

I

I

V

A
x
L

V

T

L

A

L

V

L

L

T

A

S
|
S

K
x
I

G

G

A
x
T

A

A

G
|
G

I

V

A

P

G

G

A
|
A

A
x
G

F

A

V

I

V

M

L

L

A

C

A

M

T

V

L

L

N

H

T

S

T

V

G

G

-

L

-

P

-

L

T

T

I

D

P

P

-

N

-

V

V

A

A

A

S

A

I

Y

A

A

L

M

I

I

L

L

G

G

I

I

H

D

R

A

I

I

L

L

A
x
D

E

M

G

G

L
x
R

T
|
T

F
x
M

V

V

N
|
N

L
x
V

I

T

G

G

N
x
D

A

L

L

T

A

G

T

T

V

A

V

I

V

V

A

A

K

K

W

T

E

E

G

G

K

T

L

L

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (= F57), F46 (= F57), P75 (≠ D86), L91 (≠ A103), F95 (≠ L107), L130 (≠ I149), I133 (≠ Y152), I159 (≠ V178), Y167 (≠ A186), K196 (= K207), G200 (≠ Y211), I207 (≠ L218), F210 (= F221), L250 (≠ V261), I262 (≠ L273), M269 (≠ I280), T334 (= T345), V335 (≠ F346), G336 (≠ W347), T340 (≠ G351), L343 (≠ A354), M399 (≠ F405)
binding aspartic acid: S277 (= S288), S278 (≠ T289), T314 (= T325), G354 (= G365), A358 (= A369), G359 (≠ A370), D394 (≠ A400), R397 (≠ L403), T398 (= T404)
binding sodium ion: Y89 (≠ F101), T92 (≠ I104), S93 (≠ T105), G306 (= G317), T308 (≠ S319), N310 (= N321), N310 (= N321), M311 (≠ L322), D312 (= D323), S349 (= S360), I350 (≠ K361), T352 (≠ A363), N401 (= N407), V402 (≠ L408), D405 (≠ N411)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4, 7

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
31% identity, 89% coverage: 29:428/450 of query aligns to 12:419/424 of 6zl4A

query
sites
6zl4A

L

L

W

W

F

G

Q

L

V

V

L

L

A

-

G

G

T

A

L

V

L

F

G

G

I

L

L

I

L

A

G

G

H

H

F

F

A

G

P

Y

A

A

T

G

A

A

V

V

A

K

-

T

-

Y

M

I

K

K

P

P

F

F

G

G

D

D

A

L

F

F

I

V

K

R

M

L

I

L

R

K

M

M

M

L

I

V

G

M

P

P

I

I

I

V

F

L

V

A

T

S

V

L

V

V

H

V

G

G

I

A

A

A

G

S

M

I

N

S

D

P

M

A

K

R

S

-

V

L

G

G

R

R

V

V

A

G

L

V

K

K

S

I

I

V

Y

Y

F

Y

E

L

A

A

I

T

T

S

I

A

L

M

A

A

L

V

I

F

F

F

G

G

L

L

I

I

A

V

V

G

D

R

L

L

W

F

R

N

P

V

G

G

E

A

G

N

M

V

N

N

I

L

D

G

A

S

-

G

-

T

-

G

T

K

A

A

I

I

D

E

T

-

A

-

S

-

I

-

K

-

T

-

Y

-

L

-

A

-

T

-

A

A

H

Q

D

P

Q

P

S

S

I

L

S

V

S

Q

Y

T

F

L

L

L

D

N

I

I

I

V

P

P

D

T

T

N

F

P

A

F

S

A

A

S

L

L

T

A

E

K

G

G

H

E

V

V

L

L

Q

P

V

V

L

I

F

F

I

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

A

T

A

Y

I

L

G

M

E

N

R

R

G

N

R

E

P

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

-

T

V

L

F

L

Q

R

V

V

I

F

E

D

S

G

A

L

S

A

Q

E

G

A

F

M

F

Y

K
x
L

I

I

I

V

G

G

Y
x
G

I

V

M

M

Y

Q

F

Y

A

A

P

P

L

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

V

M

G

A

Q

E

F

Q

G

G

T

V

A

R

S

V

L

V

W

G

S

P

L

L

G

A

E

K

L

-

I

-

E

-

F

-

V

V

C

G

A

A

L

V

F

Y

T

T

T

G

I

L

V

F

L

L

G

Q

S

I

V

V

A

I

H

T

W

Y

C

F

-

I

-

L

-

K

-

V

-

F

G

G

V

I

S

D

L

P

W

I

R

K

L

F

L

I

G

R

Y

K

L

A

W

K

D

D

E

A

I

M

V

I

I

T

V

A

A

F

A

V

T

T

T
x
R

S
|
S

T
x
S

E

S

T

G

V

T

L

L

P

P

R

V

L

T

I

M

Q
x
R

-

V

K

A

M

E

R

E

K

E

A

M

G

G

C

V

E

D

E

K

S

G

V

I

V

F

G

S

F

F

V

T

I

L

P

P

A

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

C

A

L

L

Y

Y

L

Q

T

G

T

V

V

T

A

V

V

L

F

F

L

V

A

A

Q

N

A

A

T

I

N

G

T

H

H

P

L

L

T

T

F

L

W

G

H

Q

E

Q

L

L

G

V

L

V

I

V

A

L

V

T

L

A

L

V

L

L

T

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

A

P

G
|
G

A
|
A

A

G

F

A

V

I

V

M

L

L

A

A

A

M

T

V

L

L

N

Q

T

S

T

V

G

G

T

L

-

D

-

L

-

T

-

P

-

G

I

S

P

P

V

V

A

A

-

L

S

A

I

Y

A

A

L

M

I

I

L

L

G

G

I

I

H

D

R

A

I

I

L

L

A

D

E

M

G

G

L
x
R

T
|
T

F

M

V

V

N

N

L

V

I

T

G

G

N

D

A

L

L

A

A

G

T

T

V

V

V

I

V

V

A

A

K

K

W

T

E

E

G

K

K

E

L

L

D

D

E

E

Q

S

binding decyl-beta-d-maltopyranoside: L191 (≠ K207), G195 (≠ Y211), R282 (≠ Q298)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ T287), S272 (= S288), S273 (≠ T289), M307 (≠ L322), T310 (= T325), G353 (= G368), A354 (= A369), R394 (≠ L403), T395 (= T404)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
31% identity, 89% coverage: 29:428/450 of query aligns to 13:420/425 of 6zgbA

query
sites
6zgbA

L

L

W

W

F

G

Q

L

V

V

L

L

A

-

G

G

T

A

L

V

L

F

G

G

I

L

L

I

L

A

G

G

H

H

F

F

A

G

P

Y

A

A

T

G

A

A

V

V

A

K

-

T

-

Y

M

I

K

K

P

P

F

F

G

G

D

D

A

L

F

F

I

V

K

R

M

L

I

L

R

K

M

M

M

L

I

V

G

M

P

P

I

I

I

V

F

L

V

A

T

S

V

L

V

V

H

V

G

G

I

A

A

A

G

S

M

I

N

S

D

P

M

A

K

R

S

-

V

L

G

G

R

R

V

V

A

G

L

V

K

K

S

I

I

V

Y

Y

F

Y

E

L

A

A

I

T

T

S

I

A

L

M

A

A

L

V

I

F

F

F

G

G

L

L

I

I

A

V

V

G

D

R

L

L

W

F

R

N

P

V

G

G

E

A

G

N

M

V

N

N

I

L

D

G

A

S

-

G

-

T

-

G

T

K

A

A

I

I

D

E

T

-

A

-

S

-

I

-

K

-

T

-

Y

-

L

-

A

-

T

-

A

A

H

Q

D

P

Q

P

S

S

I

L

S

V

S

Q

Y

T

F

L

L

L

D

N

I

I

I

V

P

P

D

T

T

N

F

P

A

F

S

A

A

S

L

L

T

A

E

K

G

G

H

E

V

V

L

L

Q

P

V

V

L

I

F

F

I

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

A

T

A

Y

I

L

G

M

E

N

R

R

G

N

R

E

P

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

-

T

V

L

F

L

Q

R

V

V

I

F

E

D

S

G

A

L

S

A

Q

E

G

A

F

M

F

Y

K

L

I

I

I

V

G

G

Y

G

I

V

M

M

Y

Q

F

Y

A

A

P

P

L

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

V

M

G

A

Q

E

F

Q

G

G

T

V

A

R

S

V

L

V

W

G

S

P

L

L

G

A

E

K

L

-

I

-

E

-

F

-

V

V

C

G

A

A

L

V

F

Y

T

T

T

G

I

L

V

F

L

L

G

Q

S

I

V

V

A

I

H

T

W

Y

C

F

-

I

-

L

-

K

-

V

-

F

G

G

V

I

S

D

L

P

W

I

R

K

L

F

L

I

G

R

Y

K

L

A

W

K

D

D

E

A

I

M

V

I

I

T

V

A

A

F

A

V

T

T

T
x
R

S
|
S

T
x
S

E

S

T

G

V

T

L

L

P

P

R

V

L

T

I

M

Q

R

-

V

K

A

M

E

R

E

K

E

A

M

G

G

C

V

E

D

E

K

S

G

V

I

V

F

G

S

F

F

V

T

I

L

P

P

A

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T

T

C

A

L

L

Y

Y

L

Q

T

G

T

V

V

T

A

V

V

L

F

F

L

V

A

A

Q

N

A

A

T

I

N

G

T

H

H

P

L

L

T

T

F

L

W

G

H

Q

E

Q

L

L

G

V

L

V

I

V

A

L

V

T

L

A

L

V

L

L

T

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

A

P

G

G

A

A

A

G

F

A

V

I

V

M

L

L

A

A

A

M

T

V

L

L

N

Q

T

S

T

V

G

G

T

L

-

D

-

L

-

T

-

P

-

G

I

S

P

P

V

V

A

A

-

L

S

A

I

Y

A

A

L

M

I

I

L

L

G

G

I

I

H

D

R

A

I

I

L

L

A
x
D

E

M

G

G

L
x
R

T
|
T

F

M

V

V

N

N

L

V

I

T

G

G

N

D

A

L

L

A

A

G

T

T

V

V

V

I

V

V

A

A

K

K

W

T

E

E

G

K

K

E

L

L

D

D

E

E

Q

S

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ T287), S273 (= S288), S274 (≠ T289), M308 (≠ L322), D392 (≠ A400), R395 (≠ L403), T396 (= T404)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
31% identity, 89% coverage: 29:428/450 of query aligns to 15:422/427 of 5e9sA

query
sites
5e9sA

L

L

W

W

F

G

Q

L

V

V

L

L

A

-

G

G

T

A

L

V

L

F

G

G

I

L

L

I

L

A

G

G

H

H

F

F

A

G

P

Y

A

A

T

G

A

A

V

V

A

K

-

T

-

Y

M

I

K

K

P

P

F

F

G

G

D

D

A

L

F

F

I

V

K

R

M

L

I

L

R

K

M

M

M

L

I

V

G

M

P

P

I

I

I

V

F

L

V

A

T

S

V

L

V

V

H

V

G

G

I

A

A

A

G

S

M

I

N

S

D

P

M

A

K

R

S

-

V

L

G

G

R

R

V

V

A

G

L

V

K

K

S

I

I

V

Y

Y

F
x
Y

E

L

A

A

I
x
T

T
x
S

I

A

L

M

A

A

L

V

I

F

F

F

G

G

L

L

I

I

A

V

V

G

D

R

L

L

W

F

R

N

P

V

G

G

E

A

G

N

M

V

N

N

I

L

D

G

A

S

-

G

-

T

-

G

T

K

A

A

I

I

D

E

T

-

A

-

S

-

I

-

K

-

T

-

Y

-

L

-

A

-

T

-

A

A

H

Q

D

P

Q

P

S

S

I

L

S

V

S

Q

Y

T

F

L

L

L

D

N

I

I

I

V

P

P

D

T

T

N

F

P

A

F

S

A

A

S

L

L

T

A

E

K

G

G

H

E

V

V

L

L

Q

P

V

V

L

I

F

F

I

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

A

T

A

Y

I

L

G

M

E

N

R

R

G

N

R

E

P

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

-

T

V

L

F

L

Q

R

V

V

I

F

E

D

S

G

A

L

S

A

Q

E

G

A

F

M

F

Y

K
x
L

I

I

I

V

G

G

Y
x
G

I

V

M

M

Y

Q

F
x
Y

A

A

P

P

L

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

V

M

G

A

Q

E

F

Q

G

G

T

V

A

R

S

V

L

V

W

G

S

P

L

L

G

A

E

K

L

-

I

-

E

-

F

-

V

V

C

G

A

A

L

V

F

Y

T

T

T

G

I

L

V

F

L

L

G

Q

S

I

V

V

A

I

H

T

W

Y

C

F

-

I

-

L

-

K

-

V

-

F

G

G

V

I

S

D

L

P

W

I

R

K

L

F

L

I

G

R

Y

K

L

A

W

K

D

D

E

A

I

M

V

I

I

T

V

A

A

F

A

V

T

T

T
x
R

S
|
S

T
x
S

E

S

T

G

V

T

L

L

P

P

R

V

L

T

I

M

Q

R

-

V

K

A

M

E

R

E

K

E

A

M

G

G

C

V

E

D

E

K

S

G

V

I

V

F

G

S

F

F

V

T

I

L

P

P

A

L

G
|
G

Y
x
A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

T
|
T

C

A

L

L

Y

Y

L

Q

T

G

T

V

V

T

A

V

V

L

F

F

L

V

A

A

Q

N

A

A

T

I

N

G

T

H

H

P

L

L

T

T

F

L

W

G

H

Q

E

Q

L

L

G

V

L

V

I

V

A

L

V

T

L

A

L

V

L

L

T

A

S
|
S

K
x
I

G
|
G

A
x
T

A

A

G
|
G

I
x
V

A

P

G

G

A
|
A

A
x
G

F

A

V

I

V

M

L

L

A

A

A

M

T

V

L

L

N

Q

T

S

T

V

G

G

T

L

-

D

-

L

-

T

-

P

-

G

I

S

P

P

V

V

A

A

-

L

S

A

I

Y

A

A

L

M

I

I

L

L

G

G

I

I

H

D

R

A

I

I

L

L

A
x
D

E

M

G

G

L
x
R

T
|
T

F

M

V

V

N
|
N

L
x
V

I

T

G

G

N
x
D

A

L

L

A

A

G

T

T

V

V

V

I

V

V

A

A

K

K

W

T

E

E

G

K

K

E

L

L

D

D

E

E

Q

S

binding aspartic acid: R274 (≠ T287), S275 (= S288), S276 (≠ T289), T313 (= T325), G353 (= G365), V354 (≠ I366), A357 (= A369), G358 (≠ A370), D394 (≠ A400), R397 (≠ L403), T398 (= T404)
binding decyl-beta-d-maltopyranoside: L194 (≠ K207), G198 (≠ Y211), Y202 (≠ F215)
binding sodium ion: Y87 (≠ F101), T90 (≠ I104), S91 (≠ T105), S276 (≠ T289), G305 (= G317), A306 (≠ Y318), T307 (≠ S319), N309 (= N321), N309 (= N321), M310 (≠ L322), D311 (= D323), S348 (= S360), I349 (≠ K361), G350 (= G362), T351 (≠ A363), N401 (= N407), V402 (≠ L408), D405 (≠ N411)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
31% identity, 89% coverage: 29:428/450 of query aligns to 15:422/426 of 6xwnB

query
sites
6xwnB

L

L

W

W

F

G

Q

L

V

V

L

L

A

-

G

G

T

A

L

V

L

F

G

G

I

L

L

I

L

A

G

G

H

H

F

F

A

G

P

Y

A

A

T

G

A

A

V

V

A

K

-

T

-

Y

M

I

K

K

P

P

F

F

G

G

D

D

A

L

F

F

I

V

K

R

M

L

I

L

R

K

M

M

M

L

I

V

G

M

P

P

I

I

I

V

F

L

V

A

T

S

V

L

V

V

H

V

G

G

I

A

A

A

G

S

M

I

N

S

D

P

M

A

K

R

S

-

V

L

G

G

R

R

V

V

A

G

L

V

K

K

S

I

I

V

Y

Y

F

Y

E

L

A

A

I

T

T

S

I

A

L

M

A

A

L

V

I

F

F

F

G

G

L

L

I

I

A

V

V

G

D

R

L

L

W

F

R

N

P

V

G

G

E

A

G

N

M

V

N

N

I

L

D

G

A

S

-

G

-

T

-

G

T

K

A

A

I

I

D

E

T

-

A

-

S

-

I

-

K

-

T

-

Y

-

L

-

A

-

T

-

A

A

H

Q

D

P

Q

P

S

S

I

L

S

V

S

Q

Y

T

F

L

L

L

D

N

I

I

I

V

P

P

D

T

T

N

F

P

A

F

S

A

A

S

L

L

T

A

E

K

G

G

H

E

V

V

L

L

Q

P

V

V

L

I

F

F

I

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

A

T

A

Y

I

L

G

M

E

N

R

R

G

N

R

E

P

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

-

T

V

L

F

L

Q

R

V

V

I

F

E

D

S

G

A

L

S

A

Q

E

G

A

F

M

F

Y

K

L

I

I

I

V

G

G

Y

G

I

V

M

M

Y

Q

F

Y

A

A

P

P

L

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

V

M

G

A

Q

E

F

Q

G

G

T

V

A

R

S

V

L

V

W

G

S

P

L

L

G

A

E

K

L

-

I

-

E

-

F

-

V

V

C

G

A

A

L

V

F

Y

T

T

T

G

I

L

V

F

L

L

G

Q

S

I

V

V

A

I

H

T

W

Y

C

F

-

I

-

L

-

K

-

V

-

F

G

G

V

I

S

D

L

P

W

I

R

K

L

F

L

I

G

R

Y

K

L

A

W

K

D

D

E

A

I

M

V

I

I

T

V

A

A

F

A

V

T

T

T

R

S
|
S

T
x
S

E

S

T

G

V

T

L

L

P

P

R

V

L

T

I

M

Q

R

-

V

K

A

M

E

R

E

K

E

A

M

G

G

C

V

E

D

E

K

S

G

V

I

V

F

G

S

F

F

V

T

I

L

P

P

A

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

C

A

L

L

Y

Y

L

Q

T

G

T

V

V

T

A

V

V

L

F

F

L

V

A

A

Q

N

A

A

T

I

N

G

T

H

H

P

L

L

T

T

F

L

W

G

H

Q

E

Q

L

L

G

V

L

V

I

V

A

L

V

T

L

A

L

V

L

L

T

A

S

S

K

I

G

G

A

T

A

A

G

G

I

V

A

P

G

G

A

A

A

G

F

A

V

I

V

M

L

L

A

A

A

M

T

V

L

L

N

Q

T

S

T

V

G

G

T

L

-

D

-

L

-

T

-

P

-

G

I

S

P

P

V

V

A

A

-

L

S

A

I

Y

A

A

L

M

I

I

L

L

G

G

I

I

H

D

R

A

I

I

L

L

A
x
D

E

M

G

G

L
x
R

T

T

F

M

V

V

N

N

L

V

I

T

G

G

N

D

A

L

L

A

A

G

T

T

V

V

V

I

V

V

A

A

K

K

W

T

E

E

G

K

K

E

L

L

D

D

E

E

Q

S

binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S288), S276 (≠ T289), M310 (≠ L322), T313 (= T325), D394 (≠ A400), R397 (≠ L403)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
30% identity, 89% coverage: 29:428/450 of query aligns to 8:411/416 of 6r7rA

query
sites
6r7rA

L

L

W

W

F

G

Q

L

V

V

L

L

A

-

G

G

T

A

L

V

L

F

G

G

I

L

L

I

L

A

G

G

H
|
H

F

F

A

G

P

Y

A

A

T

G

A

A

V

V

A

K

-

T

-

Y

M

I

K

K

P

P

F

F

G

G

D

D

A

L

F

F

I

V

K

R

M

L

I

L

R

K

M

M

M

L

I

V

G

M

P

P

I

I

I

V

F

L

V

A

T

S

V

L

V

V

H

V

G

G

I

A

A

A

G

S

M

I

N

S

D

P

M

A

K

R

S

-

V

L

G

G

R

R

V

V

A

G

L

V

K

K

S

I

I

V

Y

Y

F

Y

E

L

A

A

I

T

T

S

I

A

L

M

A

A

L

V

I

F

F

F

G

G

L

L

I

I

A

V

V

G

D

R

L

L

W

F

R

N

P

V

G

G

E

A

G

N

M

V

N

N

I

L

D

G

A

S

T

G

A

T

I

-

D

-

T

-

A

-

S

-

I

-

K

-

T

-

Y

-

L

-

A

-

T

G

A

A

H

Q

D

P

Q

P

S

S

I

L

S

V

S

Q

Y

T

F

L

L

L

D

N

I

I

I

V

P

P

D

T

T

N

F

P

A

F

S

A

A

S

L

L

T

A

E

K

G

G

H

E

V

V

L

L

Q

P

V

V

L

I

F

F

I

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

A

T

A

Y

I

L

G

M

E

N

R

R

G

N

R

E

P

E

-

R

-

V

-

R

-

K

-

S

-

A

-

E

-

T

V

L

F

L

Q

R

V

V

I

F

E

D

S

G

A

L

S

A

Q

E

G

A

F

M

F

Y

K

L

I

I

I

V

G

G

Y

G

I

V

M

M

Y

Q

F

Y

A

A

P

P

L

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

V

M

G

A

Q

E

F

Q

G

G

T

V

A

R

S

V

L
x
V

W

G

S

P

L

L

G
x
A

E

K

L

-

I

-

E

-

F

-

V

V

C

G

A

A

L

V

F

Y

T

T

T

G

I

L

V

F

L

L

G

Q

S

I

V

V

A

I

H

T

W

Y

C

F

-

I

-

L

-

K

-

V

-

F

G

G

V

I

S

D

L

P

W

I

R

K

L

F

L

I

G

R

Y

K

L

A

W

K

D

D

E

A

I

M

V

I

I

T

V

A

A

F

A

V

T

T

T
x
R

S

S

T
x
S

E

S

T

G

V

T

L

L

P

P

R

V

L

T

I

M

Q

R

-

V

K

A

M

E

R

E

K

E

A

M

G

G

C

V

E

D

E

K

S

G

V

I

V

F

G

S

F

F

V

T

I

L

P

P

A

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

C

A

L

L

Y

Y

L

Q

T

G

T

V

V

T

A

V

V

L

F

F

L

V

A

A

Q

N

A

A

T

I

N

G

T

H

H

P

L

L

T

T

F

L

W

G

H

Q

E

Q

L

L

G

V

L

V

I

V

A

L

V

T

L

A

L

V

L

L

T

A

S

S

K

I

G

G

A
x
T

A

A

G
|
G

I
x
V

A

P

G

G

A

A

A
x
G

F

A

V

I

V

M

L

L

A

A

A

M

T

V

L

L

N

Q

T

S

T

V

G

G

T

L

-

D

-

L

-

T

-

P

-

G

I

S

P

P

V

V

A

A

-

L

S

A

I

Y

A

A

L

M

I

I

L

L

G

G

I

I

H

D

R

A

I

I

L

L

A
x
D

E

M

G

G

L
x
R

T
|
T

F

M

V

V

N
|
N

L

V

I

T

G

G

N

D

A

L

L

A

A

G

T

T

V

V

V

I

V

V

A

A

K

K

W

T

E

E

G

K

K

E

L

L

D

D

E

E

Q

S

binding d-aspartic acid: R263 (≠ T287), S265 (≠ T289), M299 (≠ L322), T302 (= T325), T340 (≠ A363), G342 (= G365), V343 (≠ I366), G347 (≠ A370), D383 (≠ A400), R386 (≠ L403), T387 (= T404), N390 (= N407)
binding decyl-beta-d-maltopyranoside: H23 (= H45), V212 (≠ L236), A216 (≠ G240)

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
29% identity, 89% coverage: 22:422/450 of query aligns to 6:413/413 of 6x14A

query
sites
6x14A

R

E

F

Y

G

P

L

V

F

L

G

Q

Q

K

L
x
I

W

L

F

I

Q

G

V

L

L

I

A

L

G

G

T

A

L

I

L

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

A

G

P

Y

A

A

T

H

A

A

V

V

A

H

-

T

-

Y

M

V

K

K

P

P

F

F

G

G

D

D

A

L

F

F

I

V

K

R

M

L

I

L

R

C

M

M

M

L

I

V

G

M

P

P

I

I

I

V

F

F

V

A

T

S

V

L

V

V

H

V

G
|
G

I

A

A

A

G

S

M

I

N

S

D

P

M

A

K

R

S

-

V

L

G

G

R

R

V

V

A

G

L

V

K

K

S

I

I
x
V

I

V

Y

Y

F

Y

E

L

A

L

I

T

T

S

I

A

L

F

A

A

L

V

I

T

F

L

G

G

L

I

I

I

A

M

V

A

D

R

L

L

W

F

R

N

P

P

G

G

E

A

G

G

M

I

N

H

I

L

D

-

A

-

T

-

A

A

I

V

D

G

T

G

A

Q

S

Q

I

F

K

Q

T

P

Y

H

L

Q

A

A

T

-

A

-

H

-

D

-

Q

P

S

P

I

L

S

V

S

H

Y

I

F

L

L

L

D

D

I

I

I

V

P

P

D

T

T

N

F

P

A

F

S

G

A

A

L

L

T

A

E

N

G

G

H

Q

V

V

L

L

Q

P

V

T

L

I

F

F

I

F

S

A

V

I

L
x
I

F

L

G

G

I

I

A

A

I

I

A

T

A
x
Y

I

L

-

M

-

N

-

S

-

E

G

N

E

E

R

K

G

V

R

R

P

K

-

S

-

A

-

E

-

T

V

L

F

L

Q

D

V

A

I

I

E

N

S

G

A

L

S

A

Q

E

G

A

F

M

F

Y

K
|
K

I

I

I

V

G

N

Y

G

I

V

M
|
M

Y

Q

F

Y

A

A

P

P

L

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

V

M

G

A

Q

E

F

Q

G

G

T

V

A

H

S

V

L

V

W

G

S

E

L

L

G

A

E

K

L

V

I

T

I

A

E

A

F

V

F

Y

V

V

V

G

C

L

A

T

L

L

F

Q

T

I

T

L

I

L

V

V

L

Y

G

F

S

V

V

L

A

L

H

K

W

I

C

Y

G

G

V

I

S

D

L

P

W

I

R

S

L

F

L

I

G

K

Y

H

L

A

W

K

D

D

E

A

I

M

V

L

I

T

V

A

A

F

A

V

T

T

T

R

S

S

T
x
S

E

S

T

G

V

T

L

L

P

P

R

V

L

T

I

M

Q

R

K

V

M

A

R

K

K

E

A

M

G

G

C

I

E

S

E

E

S

G

V

I

V

Y

G

S

F

F

V

T

I

L

P

P

A

L

G

G

Y

A

S
x
T

F
x
I

N

N

L

M

D

D

G

G

T
|
T

C

A

L

L

Y

Y

L

Q

T

G

T

V

V

A

A

T

V

F

F

F

L

I

A

A

Q

N

A

A

T

L

N

G

T

S

H

H

L

L

T

T

F

V

W

G

H

Q

E

Q

L

L

G

T

L

I

I

V

A

L

V

T

L

A

L

V

L

L

T

A

S

S

K
x
I

G

G

A

T

A

A

G

G

I

V

A

P

G

G

A

A

A
x
G

F

A

V

I

V

M

L

L

A

C

A

M

T

V

L

L

N

H

T

S

T

V

G

G

-

L

-

P

-

L

T

T

I

D

P

P

-

N

-

V

V

A

A

A

S

A

I

Y

A

A

L

M

I

I

L
|
L

G

G

I

I

H

D

R

A

I

I

L

L

A
x
D

E

M

G

G

L
x
R

T

T

F

M

V

V

N

N

L

V

I

T

G

G

N

D

A

L

L

T

A

G

T

T

V

A

V

I

V

V

A

A

K

K

W

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G80), V83 (≠ I98), I157 (≠ L179), Y164 (≠ A186), K193 (= K207), T305 (≠ S319), I306 (≠ F320), I347 (≠ K361)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ L29), M199 (= M213), S275 (≠ T289), T311 (= T325), G356 (≠ A370), L384 (= L393), D391 (≠ A400), R394 (≠ L403)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
28% identity, 89% coverage: 22:422/450 of query aligns to 1:396/396 of 6bmiA

query
sites
6bmiA

R

E

F

Y

G

P

L

V

F

L

G

Q

Q

K

L

I

W

L

F

I

Q

G

V

L

L

I

A

L

G

G

T

A

L

I

L

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

A

G

P

Y

A

A

T

D

A

A

V

V

A

K

-

T

-

Y

M

V

K

K

P

P

F

F

G

G

D

D

A

L

F

F

I

V

K

R

M

L

I

L

R

K

M

M

M

L

I

V

G

M

P

P

I

I

I

V

F

F

V

A

T

S

V

L

V

V

H

V

G

G

I

A

A

A

G

S

M

I

N

S

D

P

M

A

K

R

S

-

V

L

G

G

R

R

V

V

A

G

L

V

K

K

S

I

I

V

Y

Y

F

Y

E

L

A

L

I

T

T

S

I

A

L

F

A

A

L

V

I

T

F

L

G

G

L

I

I

I

A

M

V

A

D

R

L

L

W

F

R

N

P

P

G

G

E

A

G

G

M

I

N

H

I

L

D

-

A

-

T

-

A

A

I

V

D

G

T

G

A

Q

S

Q

I

F

K

Q

T

P

Y

K

L

Q

A

A

T

-

A

-

H

-

D

-

Q

P

S

P

I

L

S

V

S

K

Y

I

F

L

L

L

D

D

I

I

I

V

P

P

D

T

T

N

F

P

A

F

S

G

A

A

L

L

T

A

E

N

G

G

H

Q

V

V

L

L

Q

P

V

T

L

I

F

F

I

F

S

A

V

I

L

I

F

L

G

G

I

I

A

A

I

I

A

T

A

Y

I

L

-

M

-

N

-

S

-

E

G

N

E

E

R

K

G

V

R

R

P

K

-

S

-

A

-

E

-

T

V

L

F

L

Q

D

V

A

I

I

E

N

S

G

A

L

S

A

Q

E

G

A

F

M

F

Y

K

K

I

I

I

V

G

N

Y

G

I

V

M

M

Y

Q

F

Y

A

A

P

P

L

I

G

G

A

V

F

F

G

A

A

L

I

I

A

A

F

Y

T

V

V

M

G

A

Q

E

F

Q

G

G

T

V

A

K

S

V

L

V

W

G

S

E

L

L

G

A

E

K

L

V

I

T

I

A

E

A

F

V

F

Y

V

V

V

G

C

L

A

T

L

L

F

Q

T

I

T

L

I

L

V

V

L

Y

G

F

S

V

V

L

A

L

H

K

W

I

C

Y

G

G

V

I

S

D

L

P

W

I

R

S

L

F

L

I

G

K

Y

K

L

A

W

K

D

D

E

A

I

M

V

L

I

T

V

A

A

F

A

V

T

T

T
x
R

S

S

T
x
S

E

S

T

G

V

T

L

L

P

P

R

V

L

T

I

M

Q

R

K

V

M

A

R

K

K

E

A

M

G

G

C

I

E

S

E

E

S

G

V

I

V

Y

G

S

F

F

V

T

I

L

P

P

A

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

T
|
T

C

A

L

L

Y

Y

L

Q

T

G

T

V

V

C

A

T

V

F

F

F

L

I

A

A

Q

N

A

A

T

L

N

G

T

S

H

H

L

L

T

T

F

V

W

G

H

Q

E

Q

L

L

G

T

L

I

I

V

A

L

V

T

L

A

L

V

L

L

T

A

S

-

K

-

G

-

A

-

G

-

I

-

A

-

G

-

A

-

F

-

V

I

V

M

L

L

A

A

A

M

T

V

L

L

N

E

T

S

T

V

G

G

-

L

-

P

-

L

T

T

I

D

P

P

-

N

-

V

V

A

A

A

S

A

I

Y

A

A

L

M

I

I

L

L

G

G

I

I

H

D

R

A

I

I

L

L

A
x
D

E

M

G

G

L

C

T
|
T

F

M

V

V

N
|
N

L

V

I

T

G

G

N

D

A

L

L

T

A

G

T

T

V

A

V

I

V

V

A

A

K

K

W

T

E

E

binding serine: R268 (≠ T287), S270 (≠ T289), T306 (= T325), D374 (≠ A400), T378 (= T404), N381 (= N407)

7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
26% identity, 87% coverage: 36:428/450 of query aligns to 33:409/412 of 7awmA

query
sites
7awmA

G

G

T

V

L

V

L

L

G

G

I

F

L

L

G

R

H

P

F

Y

A

-

P

P

A

L

T

S

A

P

V

R

A

E

M

V

K

K

P

Y

F

F

-

A

-

F

-

P

G

G

D

E

A

L

F

L

I

M

K

R

M

M

I

L

R

K

M

M

M

L

I

I

G

L

P

P

I

L

I

I

F

V

V

S

T

S

V

L

V

I

H

T

G

G

I

L

A

A

G

S

M

L

N

-

D

D

M

A

K

K

S

A

V
x
S

G
|
G

R

R

V

L

A
x
G

L

M

K

R

S
x
A

I
x
V

I

V

Y

Y

F
x
Y

E

M

A

S

I

T

T

T

I

I

L

I

A

A

L

V

I

V

F

L

G

G

L

I

I

I

A

L

V

V

D

L

L

I

W

I

R

H

P

P

G

-

E

-

G

-

M

-

N

-

I

-

D

-

A

-

T

-

A

-

I

-

D

-

T

-

A

E

S

V

I

L

K

D

T

C

Y

F

L

L

A

D

T

L

A

A

H

R

D

N

-

I

-

F

-

P

-

S

Q

N

S

L

I

V

S

S

S

A

Y

A

F

F

L

-

D

-

I

-

P

-

D

R

T

S

F

Y

A

S

S

T

A

Q

L

E

T

V

E

E

G

G

-

M

H

N

V

I

L

L

Q

G

V

L

L

V

F

V

I

F

S

S

V
x
M

L

V

F

F

G

G

I
x
F

A

A

I

L

A

G

A

K

I

M

G

G

E

E

R

Q

G

G

R

Q

P

L

V

L

F

V

Q

D

V

F

I

F

E

N

S

S

A

L

S

N

Q

E

G

A

F

T

F

M

K

K

I

L

I

V

G

A

Y

I

I

I

M

M

Y

W

F

Y

A

A

P

P

L

L

G

G

A

I

F

L

G

F

A

L

I

I

A

A

F

G

T

K

V

I

-

V

-

G

G

G

Q

Q

F

L

G

G

T

M

A

Y

S

M

L

V

W

T

S

V

L

I

G

V

E

G

L

L

I

V

I

I

E

H

F

G

F

L

V

I

V

V

C

L

A

P

L

L

F

I

T

Y

T

F

I

L

V

I

L

T

G

R

S

K

V

-

A

-

H

-

W

-

C

-

G

-

V

-

S

N

L

P

W

F

R

V

L

F

L

I

G

A

Y

G

L

I

W

L

D

Q

E

A

I

L

V

I

I

T

V

A

A

L

A

G

T

T

T

S

S
|
S

T
x
S

E

S

T

A

V

T

L

L

P

P

R

I

L

T

I

F

Q

K

K

C

M

L

R

E

K

E

A

N

-

N

G

G

C

V

E

D

E

K

S

R

V

I

V

T

G

R

F
|
F

V

V

I

L

P

P

A
x
V

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

T
|
T

C

A

L

L

Y

Y

L

E

T

A

T

V

V

A

A

A

V

I

F

F

L

I

A

A

Q

Q

A

V

T

N

N

N

T

Y

H

E

L

L

T

D

F

F

W

G

H

Q

E

I

L

I

G

T

L

I

I

S

A

I

V

T

L

A

L

T

L

A

T

A

S

S

K

I

G

G

A

A

G
|
G

I
|
I

A

P

G

Q

A
|
A

A
x
G

F

L

V

V

V

T

L

M

A

V

A

I

T

V

L

L

N

T

T

A

T

V

G

G

T

-

I

L

P

P

V

T

A

D

S

D

I

I

A

T

L

L

I

I

L

I

G

A

I

V

H

D

R

W

I

L

L

L

A
x
D

E

R

G

F

L
x
R

T
|
T

F

M

V

V

N

N

L

V

I

L

G

G

N

D

A

A

L

L

A

G

T

A

V

G

V

I

V

V

A

E

K

H

W

L

E

S

G

R

K

K

-

E

L

L

D

E

E

K

Q

Q

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ V90), G89 (= G91), G92 (≠ A94), A95 (≠ S97), V96 (≠ I98), Y99 (≠ F101), M163 (≠ V178), F167 (≠ I182), F293 (= F312), V297 (≠ A316)
binding aspartic acid: S268 (= S288), S269 (≠ T289), T306 (= T325), G346 (= G365), I347 (= I366), A350 (= A369), G351 (≠ A370), D380 (≠ A400), R383 (≠ L403), T384 (= T404)

5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
26% identity, 87% coverage: 36:428/450 of query aligns to 25:395/397 of 5mjuA

query
sites
5mjuA

G

G

T

V

L

V

L

L

G

G

I

F

L

L

G

R

H

P

F

Y

A

-

P

P

A

L

T

S

A

P

V

R

A

E

M

V

K

K

P

Y

F

F

-

A

-

F

-

P

G

G

D

E

A

L

F

L

I

M

K

R

M

M

I

L

R

K

M

M

M

L

I

I

G

L

P

P

I

L

I
|
I

F

V

V

S

T

S

V

L

V

I

H

T

G

G

I
x
L

A

A

G

S

M

L

N

-

D

D

M

A

K

K

S

A

V
x
S

G
|
G

R

R

V

L

A
x
G

L

M

K

R

S

A

I

V

Y

Y

F
x
Y

E

M

A

S

I

T

T

T

I

I

L

I

A

A

L

V

I

V

F

L

G

G

L

I

I

I

A

L

V

V

-

L

-

I

-

H

-

E

-

V

-

L

-

D

-

C

-

F

-

L

D

D

L

L

W

A

R

R

P

-

G

-

E

-

G

-

M

-

N

N

I

I

D

F

A

P

T

S

A

N

I

L

D

V

T

S

A

A

S

A

I

F

K

R

T

S

Y

Y

L

S

A

T

T

T

A

G

H

Q

D

E

Q

-

S

-

I

-

S

-

Y

-

F

-

L

-

D

-

I

-

P

-

D

-

T

-

F

-

A

-

S

-

A

-

L

-

T

V

E

E

G

G

-

M

H

N

V
x
I

L

L

Q

G

V

L

L

V

F

V

I

F

S

S

V
x
M

L

V

F

F

G

G

I
x
F

A

A

I

L

A

G

A

K

I

M

G

G

E

E

R

Q

G

G

R

Q

P

L

V

L

F

V

Q

D

V

F

I

F

E

N

S

S

A

L

S

N

Q

E

G

A

F

T

F

M

K

K

I

L

I

V

G

A

Y

I

I

I

M

M

Y

W

F

Y

A

A

P

P

L

L

G

G

A

I

F

L

G

F

A

L

I

I

A

A

F

G

T

K

V

I

-

V

-

E

G

G

Q

Q

F

L

G

G

T

M

A

Y

S

M

L

V

W

T

S

V

L

I

G

V

E

G

L

L

I

V

I

I

E

H

F

G

F

L

V

I

V

V

C

L

A

P

L

L

F

I

T

Y

T

F

I

L

V

I

L

T

G

R

S

K

V

-

A

-

H

-

W

-

C

-

G

-

V

-

S

N

L

P

W

F

R

V

L

F

L

I

G

A

Y

G

L

I

W

L

D

Q

E

A

I

L

V

I

I

T

V

A

A

L

A

G

T

T

T

S

S

S

T
x
S

E
x
S

T

A

V

T

L

L

P

P

R

I

L

T

I

F

Q

K

K

C

M

L

R

E

K

E

-

N

A

N

G

G

C

V

E

D

E

K

S

R

V

I

V

T

G

R

F
|
F

V

V

I

L

P

P

A
x
V

G

G

Y
x
A

S
x
T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

C

A

L

L

Y

Y

L

E

T

A

T

V

V

A

A

A

V

I

F

F

L

I

A

A

Q

Q

A

-

T

V

N

N

T

Q

H

I

L

I

T

T

F

-

W

-

H

-

E

-

L

I

G

S

L

I

I

T

A

A

V

T

L

-

L

A

T

A

S

S

K

I

G
|
G

A

A

G

G

I

I

A

P

G

Q

A
|
A

A
x
G

F

L

V

V

V

T

L

M

A

V

A

I

T

V

L

L

N

T

T

A

T

V

G

G

T

-

I

L

P

P

V

T

A

D

S

D

I

I

A

T

L

L

I

I

L

I

G

A

I

V

H

D

R

W

I

L

L

L

A
x
D

E

R

G

F

L
x
R

T

T

F

M

V

V

N
|
N

L

V

I

L

G

G

N

D

A

A

L

L

A

G

T

A

V

G

V

I

V

V

A

E

K

H

W

L

E

S

G

R

K

K

-

E

L

L

D

E

E

K

Q

Q

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (≠ I81), S80 (≠ V90), G81 (= G91), G84 (≠ A94), Y91 (≠ F101), M156 (≠ V178), F160 (≠ I182), F286 (= F312), V290 (≠ A316)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (= I73), I148 (≠ V170), S262 (≠ T289), S263 (≠ E290), A292 (≠ Y318), T293 (≠ S319), M296 (≠ L322), T299 (= T325), G329 (= G362), A336 (= A369), G337 (≠ A370), D366 (≠ A400), R369 (≠ L403), N373 (= N407)

P56564 Excitatory amino acid transporter 1; Glial high affinity glutamate transporter; High-affinity neuronal glutamate transporter; GluT-1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Mus musculus (Mouse) (see paper)
24% identity, 92% coverage: 33:447/450 of query aligns to 58:527/543 of P56564

query
sites
P56564

V

V

L

I

A

V

G

G

T

T

L

I

L

L

G

G

I

F

L

A

L

L

G

R

H

P

F

Y

A

K

P

-

A

M

T

S

A

Y

V

R

A

E

M

V

K

K

P

Y

F

F

-

S

-

F

-

P

G

G

D

E

A

L

F

L

I

M

K

R

M

M

I

L

R

Q

M

M

M

L

I

V

G

L

P

P

I

L

I

I

F

I

V

S

T

S

V

L

V

V

H

T

G

G

I

M

A

A

G

A

M

L

N

-

D

D

M

S

K

K

S

A

V

S

G

G

R

K

V

M

A

G

L

M

K

R

S

A

I

V

Y

Y

F

Y

E

M

A

T

I

T

T

T

I

I

L

I

A

A

L

V

I

V

F

I

G

G

L

I

I

I

A

I

V

V

D

I

L

I

W

I

R

H

P

P

G

G

-

K

-

G

-

T

-

K

-

E

-

N

-

M

-

Y

-

R

E

E

G

G

M

K

N

I

I

V

D

Q

A

V

T

T

A

A

I

A

D

D

-

A

-

F

-

L

-

D

-

L

-

I

-

R

-

N

-

M

-

F

-

P

-

N

-

L

-

V

T

E

A

A

S

C

I

F

K

K

T

Q

Y

F

L

K

A

T

T

S

A

Y

H

E

D

K

Q

R

S

S

I

F

-

K

-

V

-

P

-

I

-

Q

-

S

S
x
N

S

E

Y

T

F

L

L

L

D

G

I

A

I

V

P

I

D

N

T
x
N

F

V

A

S

S

E

A

A

L

M

T

E

-

T

-

L

-

T

-

R

-

I

-

R

-

E

-

M

-

V

-

P

-

V

E

P

G

G

H

S

V

V

-

N

-

G

-

V

-

N

-

A

L

L

Q

G

V

L

L

V

F

V

I

F

S

S

V

M

L

C

F

F

G

G

I

F

A

V

I

I

A

G

A

N

I

M

G

K

E

E

R

Q

G

G

R

Q

P

A

V

L

F

R

Q

E

V

F

I

F

E

D

S

S

A

L

S

N

Q

E

G

A

F

I

F

M

K

R

I

L

I

V

G

A

Y

V

I

I

M

M

Y

W

F

Y

A

A

P

P

L

L

G

G

A

I

F

L

G

F

A

L

I

I

A

A

F

G

T

K

V

I

-

V

-

E

-

M

-

E

-

D

-

M

-

G

-

V

-

I

-

G

G

G

Q

Q

F

L

G

A

T

M

A

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W

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V

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G

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L

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A

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P

L

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Y

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L

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K

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R

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F

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V

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P

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G

Y

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N

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D

D

G

G

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C

A

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Y

Y

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A

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A

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F

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I

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Q

A

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N

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N

L

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I

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G

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D

A

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L

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A

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G

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I

V

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-

H

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A

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N

L

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D

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A

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A

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I

I

G

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A

N206 (≠ S150) modified: carbohydrate, N-linked (GlcNAc...) asparagine
N216 (≠ T160) modified: carbohydrate, N-linked (GlcNAc...) asparagine

P43003 Excitatory amino acid transporter 1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Homo sapiens (Human) (see 3 papers)
23% identity, 92% coverage: 33:444/450 of query aligns to 58:524/542 of P43003

query
sites
P43003

V

V

L

I

A

V

G

G

T

T

L

I

L

L

G

G

I

F

L

T

L

L

G

R

H

P

F

Y

A

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-

A

M

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A

Y

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A

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M

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K

K

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Y

F

F

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F

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G

G

D

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A

L

F

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M

K

R

M

M

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M

M

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G

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A

A

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D

D

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S

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|
S

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x
S

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P

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|
T

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Y

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|
I

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P

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|
A

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x
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V

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A

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x
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|
N

L

V

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L

G

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D

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L

L

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L

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x
Y

Q

Q

S363 (≠ T287) mutation to R: Loss of electrogenic glutamate transport. Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with M-477.
SSS 363:365 (≠ TST 287:289) binding L-aspartate
T396 (≠ S319) binding Na(+)
T402 (= T325) binding L-aspartate
IPQAG 443:447 (≠ IAGAA 366:370) binding L-aspartate
D476 (≠ A400) binding L-aspartate
R477 (≠ E401) mutation to M: Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with R-363.
N483 (= N407) binding L-aspartate
Y523 (≠ H443) mutation to F: No effect on activity.

8cuaA Human excitatory amino acid transporter 3 (eaat3) with bound potassium in an intermediate outward facing state (see paper)
25% identity, 83% coverage: 58:429/450 of query aligns to 43:412/416 of 8cuaA

query
sites
8cuaA

G

G

D

E

A

I

F

L

I

M

K

R

M

M

I

L

R

K

M

L

M

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P

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G

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G

G

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P

P

G

G

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S

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M

V

-

D

-

A

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M

-

L

-

D

-

L

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I

-

R

N

N

I

M

D

F

A

P

T

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A

N

I

L

D

V

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Q

A

A

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A

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Y

Y

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V

L

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A

A

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A

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I

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x
S

S
|
S

T

S

E

S

T

A

V

T

L

L

P

P

R

V

L

T

I

F

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C

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A

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N

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N

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Q

C

V

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V

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G

R

F

F

V

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P

P

A

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G

Y

A

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N

N

L

M

D

D

G

G

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A

L

L

Y

Y

L

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A

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V

V

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F

F

L

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N

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S

S

K

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G

G

A
|
A

G
|
G

I
x
V

A
x
P

G

Q

A
|
A

A

G

F

L

V

V

V

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L

M

A

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A

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A

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P

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x
D

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L

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T
|
T

F

M

V

V

N

N

L

V

I

L

G

G

N

D

A

A

L

F

A

G

T

T

V

G

V

I

V

V

A

E

K

K

W

L

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S

G

K

K

K

L

E

D

L

E

E

Q

Q

L

M

binding potassium ion: S270 (≠ T287), S271 (= S288), A347 (= A363), A348 (= A364), G349 (= G365), V350 (≠ I366), P351 (≠ A367), A353 (= A369), D383 (≠ A400), T387 (= T404)

8cv2A Human excitatory amino acid transporter 3 (eaat3) in an outward facing sodium-bound state (see paper)
24% identity, 83% coverage: 58:429/450 of query aligns to 43:430/433 of 8cv2A

query
sites
8cv2A

G

G

D

E

A

I

F

L

I

M

K

R

M

M

I

L

R

K

M

L

M

I

I

I

G

L

P

P

I

L

I

I

F

I

V

S

T

S

V

M

V

I

H

T

G

G

I

V

A

A

G

A

M

L

N

D

D

S

M

N

K

V

S

S

V

-

G

G

R

K

V

I

A

G

L

L

K

R

S

A

I

V

Y

Y

F
x
Y

E

F

A

A

I
x
T

T
|
T

I

L

L

I

A

A

L

V

I

I

F

L

G

G

L

I

I

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A

L

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A

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A

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D

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A

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I

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M

F

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A

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L

L

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A

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A

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I

I

M

M

Y

W

F

Y

A

M

P

P

L

L

G

G

A

I

F

L

G

F

A

L

I

I

A

A

F

-

T

-

V

-

G

G

Q

C

F

I

G

I

T

E

A

V

S

E

L

D

W

W

S

-

L

-

G

-

E

-

L

-

I

-

E

E

F

I

F

F

V

R

V

K

C

L

A

G

L

L

F

Y

T

M

T

A

I

T

V

V

L

L

G

T

S

G

V

L

A

A

H

I

W

H

C

S

G

I

V

V

S

I

L

L

-

P

-

L

-

I

-

Y

-

F

-

I

-

V

-

R

-

K

-

N

-

P

W

F

R

R

L

F

L

A

G

M

Y

G

L

M

W

A

D

Q

E

A

I

L

V

L

I

T

V

A

A

L

A

M

T

I

T

S

S

S

T

S

E

S

T

A

V

T

L

L

P

P

R

V

L

T

I

F

Q

R

K

C

M

A

R

E

K

E

A

N

G

N

-

Q

C

V

E

D

E

K

S

R

V

I

V

T

G

R

F

F

V

V

I

L

P

P

A

V

G
|
G

Y
x
A

S

T

F

I

N
|
N

L
x
M

D
|
D

G

G

T

T

C

A

L

L

Y

Y

L

E

T

A

T

V

V

A

A

A

V

V

F

F

L

I

A

A

Q

Q

A

L

T

N

N

D

T

L

H

D

L

L

T

G

F

I

W

G

H

Q

E

I

L

I

G

T

L

I

I

S

A

I

V

T

L

A

L

T

L

S

T

A

S

S

K

I

G

G

A

A

G

G

I

V

A

P

G

Q

A

A

A

G

F

L

V

V

V

T

L

M

A

V

A

I

T

V

L

L

N

S

T

A

T

V

G

G

T

-

I

L

P

P

V

A

A

E

S

D

I

V

A

T

L

L

I

I

L

I

G

A

I

V

H

D

R

C

I

L

L

L

A

D

E

R

G

F

L

R

T

T

F

M

V

V

N
|
N

L

V

I

L

G

G

N
x
D

A

A

L

F

A

G

T

T

V

G

V

I

V

V

A

E

K

K

W

L

E

S

G

K

K

K

L

E

D

L

E

E

Q

Q

L

M

binding sodium ion: Y85 (≠ F101), T88 (≠ I104), T89 (= T105), G319 (= G317), A320 (≠ Y318), N323 (= N321), N323 (= N321), M324 (≠ L322), D325 (= D323), N408 (= N407), D412 (≠ N411)

9d68A Human excitatory amino acid transporter 3 (eaat3) with bound l- cysteine in an outward facing state (see paper)
24% identity, 83% coverage: 58:429/450 of query aligns to 38:425/425 of 9d68A

query
sites
9d68A

G

G

D

E

A

I

F

L

I

M

K

R

M

M

I

L

R

K

M

L

M

I

I

I

G

L

P

P

I

L

I

I

F

I

V

S

T

S

V

M

V

I

H

T

G

G

I

V

A

A

G

A

M

L

N

D

D

S

M

N

K

V

S

S

V

-

G

G

R

K

V

I

A

G

L

L

K

R

S

A

I

V

Y

Y

F
x
Y

E

F

A

A

I
x
T

T
|
T

I

L

L

I

A

A

L

V

I

I

F

L

G

G

L

I

I

V

A

L

V

V

D

V

L

S

W

I

R

K

P

P

G

G

E

V

G

T

M

Q

N

K

I

V

D

G

A

E

T

I

A

A

I

-

D

-

T

-

A

-

S

-

I

-

K

-

T

-

Y

R

L

T

A

G

T

S

A

T

H

P

D

E

Q

V

S

S

I

T

S

V

S

D

Y

A

F

M

L

L

D

D

I

L

I

I

P

R

D

N

T

M

F

F

A

P

S

E

A

N

L

L

T

V

E

Q

G

A

-

A

-

F

-

Q

-

Y

-

K

-

T

-

K

-

R

-

E

-

Y

-

K

-

I

-

V

-

G

-

M

-

Y

-

S

-

D

-

G

-

I

H

N

V

V

L

L

Q

G

V

L

L

I

F

V

I

F

S

A

V

L

L

V

F

F

G

G

I

L

A

V

I

I

A

G

A

K

I

M

G

G

E

E

R

K

G

G

R

Q

P

I

V

L

F

V

Q

D

V

F

I

F

E

N

S

A

A

L

S

S

Q

D

G

A

F

T

F

M

K

K

I

I

I

V

G

Q

Y

I

I

I

M

M

Y

W

F

Y

A

M

P

P

L

L

G

G

A

I

F

L

G

F

A

L

I

I

A

A

F

-

T

-

V

-

G

G

Q

C

F

I

G

I

T

E

A

V

S

E

L

D

W

W

S

-

L

-

G

-

E

-

L

-

I

-

E

E

F

I

F

F

V

R

V

K

C

L

A

G

L

L

F

Y

T

M

T

A

I

T

V

V

L

L

G

T

S

G

V

L

A

A

H

I

W

H

C

S

G

I

V

V

S

I

L

L

-

P

-

L

-

I

-

Y

-

F

-

I

-

V

-

R

-

K

-

N

-

P

W

F

R

R

L

F

L

A

G

M

Y

G

L

M

W

A

D

Q

E

A

I

L

V

L

I

T

V

A

A

L

A

M

T

I

T

S

S
|
S

T
x
S

E

S

T

A

V

T

L

L

P

P

-

V

-

T

-

F

R

R

L

C

I

A

Q

E

K

E

M

N

R

N

K

Q

A

-

G

-

C

V

E

D

E

K

S

R

V

I

V

T

G

R

F

F

V

V

I

L

P

P

A

V

G
|
G

Y
x
A

S

T

F

I

N
|
N

L

M

D
|
D

G

G

T
|
T

C

A

L

L

Y

Y

L

E

T

A

T

V

V

A

A

A

V

V

F

F

L

I

A

A

Q

Q

A

L

T

N

N

D

T

L

H

D

L

L

T

G

F

I

W

G

H

Q

E

I

L

I

G

T

L

I

I

S

A

I

V

T

L

A

L

T

L

S

T

A

S

S

K

I

G

G

A

A

A
|
A

G

G

I

V

A

P

G

Q

A

A

A

G

F

L

V

V

V

T

L

M

A

V

A

I

T

V

L

L

N

S

T

A

T

V

G

G

T

-

I

L

P

P

V

A

A

E

S

D

I

V

A

T

L

L

I

I

L

I

G

A

I

V

H

D

R

C

I

L

L

L

A
x
D

E

R

G

F

L
x
R

T
|
T

F

M

V

V

N
|
N

L

V

I

L

G

G

N
x
D

A

A

L

F

A

G

T

T

V

G

V

I

V

V

A

E

K

K

W

L

E

S

G

K

K

K

L

E

D

L

E

E

Q

Q

L

M

binding cysteine: S284 (= S288), S285 (≠ T289), T322 (= T325), A361 (= A364), D396 (≠ A400), R399 (≠ L403), T400 (= T404), N403 (= N407)
binding sodium ion: Y80 (≠ F101), T83 (≠ I104), T84 (= T105), G314 (= G317), A315 (≠ Y318), N318 (= N321), N318 (= N321), D320 (= D323), N403 (= N407), D407 (≠ N411)

9d67A Human excitatory amino acid transporter 3 (eaat3) with bound d- aspartate in an intermediate outward facing state (see paper)
24% identity, 82% coverage: 58:424/450 of query aligns to 40:404/406 of 9d67A

query
sites
9d67A

G

G

D

E

A

I

F

L

I

M

K

R

M

M

I

L

R

K

M

L

M

I

I

I

G

L

P

P

I

L

I

I

F

I

V

S

T

S

V

M

V

I

H

T

G

G

I

V

A

A

G

A

M

L

N

D

D

S

M

N

K

V

S

S

V

-

G

G

R

K

V

I

A

G

L

L

K

R

S

A

I

V

Y

Y

F
x
Y

E

F

A

A

I
x
T

T
|
T

I

L

L

I

A

A

L

V

I

I

F

L

G

G

L

I

I

V

A

L

V

V

D

V

L

S

W

I

R

K

P

P

G

G

E

S

G

T

M

V

-

D

-

A

-

M

-

L

-

D

-

L

-

I

-

R

N

N

I

M

D

F

A

P

T

E

A

N

I

L

D

V

T

Q

A

A

S

A

I

F

K

Q

T

Q

Y

Y

L

K

A

T

T

K

A

R

H

E

D

E

Q

Y

S

K

I

I

S

V

S

G

Y

M

F

Y

L

S

D

D

I

G

I

I

P

-

D

-

T

-

F

-

A

-

S

-

A

-

L

-

T

-

E

-

G

-

H

N

V

V

L

L

Q

G

V

L

L

I

F

V

I

F

S

A

V

L

L

V

F

F

G

G

I

L

A

V

I

I

A

G

A

K

I

M

G

G

E

E

R

K

G

G

R

Q

P

I

V

L

F

V

Q

D

V

F

I

F

E

N

S

A

A

L

S

S

Q

D

G

A

F

T

F

M

K

K

I

I

I

V

G

Q

Y

I

I

I

M

M

Y

W

F

Y

A

M

P

P

L

L

G

G

A

I

F

L

G

F

A

L

I

I

A

A

F

-

T

-

V

-

G

G

Q

C

F

I

G

I

T

E

A

V

S

E

L

D

W

W

S

-

L

-

G

-

E

-

L

-

I

-

E

E

F

I

F

F

V

R

V

K

C

L

A

G

L

L

F

Y

T

M

T

A

I

T

V

V

L

L

G

T

S

G

V

L

A

A

H

I

W

H

C

S

G

I

V

V

S

I

L

L

-

P

-

L

-

I

-

Y

-

F

-

I

-

V

-

R

-

K

-

N

-

P

W

F

R

R

L

F

L

A

G

M

Y

G

L

M

W

A

D

Q

E

A

I

L

V

L

I

T

V

A

A

L

A

M

T

I

T

S

S
|
S

T
x
S

E

S

T

A

V

T

L

L

P

P

R

V

L

T

I

F

Q

R

K

C

M

A

R

E

K

E

A

N

G

N

-

Q

C

V

E

D

E

K

S

R

V

I

V

T

G

R

F

F

V

V

I

L

P

P

A

V

G
|
G

Y
x
A

S
x
T

F
x
I

N
|
N

L
x
M

D
|
D

G

G

T
|
T

C

A

L

L

Y

Y

L

E

T

A

T

V

V

A

A

A

V

V

F

F

L

I

A

A

Q

Q

A

L

T

N

N

D

T

L

H

D

L

L

T

G

F

I

W

G

H

Q

E

I

L

I

G

T

L

I

I

S

A

I

V

T

L

A

L

T

L

S

T

A

S
|
S

K
x
I

G
|
G

A
|
A

A

A

G
|
G

I
x
V

A

P

G

Q

A

A

A
x
G

F

L

V

V

V

T

L

M

A

V

A

I

T

V

L

L

N

S

T

A

T

V

G

G

T

-

I

L

P

P

V

A

A

E

S

D

I

V

A

T

L

L

I

I

L

I

G

A

I

V

H

D

R

C

I

L

L

L

A
x
D

E

R

G

F

L
x
R

T
|
T

F

M

V

V

N
|
N

L

V

I

L

G

G

N
x
D

A

A

L

F

A

G

T

T

V

G

V

I

V

V

A

E

K

K

W

L

E

S

G

K

K

K

binding d-aspartic acid: S268 (= S288), S269 (≠ T289), T306 (= T325), G346 (= G365), V347 (≠ I366), G351 (≠ A370), D380 (≠ A400), R383 (≠ L403), T384 (= T404), N387 (= N407)
binding sodium ion: Y82 (≠ F101), T85 (≠ I104), T86 (= T105), S269 (≠ T289), G298 (= G317), A299 (≠ Y318), T300 (≠ S319), I301 (≠ F320), N302 (= N321), N302 (= N321), M303 (≠ L322), M303 (≠ L322), D304 (= D323), S341 (= S360), I342 (≠ K361), G343 (= G362), A344 (= A363), N387 (= N407), D391 (≠ N411)

Query Sequence

>WP_280115907.1 NCBI__GCF_000009265.1:WP_280115907.1
MTYLEASKTMTPVGETQKPAGRFGLFGQLWFQVLAGTLLGILLGHFAPATAVAMKPFGDA
FIKMIRMMIGPIIFVTVVHGIAGMNDMKSVGRVALKSIIYFEAITILALIFGLIAVDLWR
PGEGMNIDATAIDTASIKTYLATAHDQSISSYFLDIIPDTFASALTEGHVLQVLFISVLF
GIAIAAIGERGRPVFQVIESASQGFFKIIGYIMYFAPLGAFGAIAFTVGQFGTASLWSLG
ELIIEFFVVCALFTTIVLGSVAHWCGVSLWRLLGYLWDEIVIVAATTSTETVLPRLIQKM
RKAGCEESVVGFVIPAGYSFNLDGTCLYLTTVAVFLAQATNTHLTFWHELGLIAVLLLTS
KGAAGIAGAAFVVLAATLNTTGTIPVASIALILGIHRILAEGLTFVNLIGNALATVVVAK
WEGKLDEQLLAATIGNDRLRARHQTAAAAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory