SitesBLAST

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 88% coverage: 14:241/259 of query aligns to 4:226/393 of P9WQI3

query
sites
P9WQI3

I

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H

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N

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P

H193 (= H207) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 91% coverage: 8:243/259 of query aligns to 12:241/378 of P69874

query
sites
P69874

T

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N

C26 (≠ R22) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y23) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ L42) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C51) mutation to T: Loss of ATPase activity and transport.
L60 (≠ M57) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ V73) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V134) mutation to M: Loss of ATPase activity and transport.
D172 (= D173) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
42% identity, 90% coverage: 12:243/259 of query aligns to 1:226/358 of 8y5iA

query
sites
8y5iA

P

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M

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x
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x
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T

N

binding adenosine-5'-triphosphate: F12 (≠ Y23), K15 (≠ T27), G38 (= G50), K41 (= K53), T42 (= T54), T43 (= T55), R128 (= R144), Q132 (≠ E148), S134 (= S150)

1g291 Malk (see paper)
42% identity, 85% coverage: 29:247/259 of query aligns to 18:237/372 of 1g291

query
sites
1g291

A

A

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M

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M

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N

S

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A

binding magnesium ion: D69 (= D82), E71 (≠ V84), K72 (≠ R85), K79 (≠ L86), D80 (≠ R87)
binding pyrophosphate 2-: S38 (= S49), G39 (= G50), C40 (= C51), G41 (= G52), K42 (= K53), T43 (= T54), T44 (= T55)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

8hprC Lpqy-sugabc in state 4 (see paper)
40% identity, 94% coverage: 14:257/259 of query aligns to 3:236/363 of 8hprC

query
sites
8hprC

I

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K

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H
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H

D

D

L

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E

A

A

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M

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L

L

G

G

D

D

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M

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A

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Q

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A

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E

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A

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R

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P

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S

S

P

P

A

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N

P

L

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F

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T

A

G

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C

I

G

G

G

S

binding adenosine-5'-triphosphate: Y12 (= Y23), S38 (= S49), G39 (= G50), G41 (= G52), K42 (= K53), S43 (≠ T54), Q82 (= Q95), Q133 (≠ E148), G136 (= G151), G137 (= G152), Q138 (= Q153), H192 (= H207)
binding magnesium ion: S43 (≠ T54), Q82 (= Q95)

8hprD Lpqy-sugabc in state 4 (see paper)
40% identity, 94% coverage: 14:257/259 of query aligns to 3:236/362 of 8hprD

query
sites
8hprD

I

I

S

V

L

L

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D

G

R

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V

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T

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K

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Y
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Y

P

P

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D

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A

A

A

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V

A

K

D

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A

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L

F

L

I

C

I

L

L

V

V

G

G

P

P

S
|
S

G

G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

T

M

L

K

N

M

M

I

I

N

A

R

G

L

L

V

E

E

E

P

I

T

T

S

S

G

G

H

E

I

L

L

R

V

I

A

G

G

G

E

E

D

R

V

V

A

N

R

E

T

K

D

A

P

P

V

K

R

D

L

-

R

-

R

R

R

D

I

I

G

A

Y

M

V

V

I

F

Q
|
Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

H

H

L

M

T

T

V

V

H

R

A

Q

N

N

V

I

G

A

T

F

V

P

P

L

R

T

M

L

L

A

G

K

W

V

D

P

R

K

A

A

R

E

I

I

R

A

A

A

R

K

V

V

D

E

A

E

M

T

L

A

D

K

L

I

V

-

G

-

L

L

D

D

P

L

A

S

T

E

M

L

G

L

R

D

R
|
R

Y

K

P

P

H

G

E
x
Q

L

L

S
|
S

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

G

A

V

M

A

G

R

R

A

A

L

I

A

V

A

R

D

S

P

P

P

K

V

A

L

F

L

L

M

M

D

D

E
x
Q

P

P

F

L

S

S

A

N

I

L

D

D

P

A

I

K

A

L

R

R

D

V

R

Q

L

M

Q

R

A

A

E

E

F

I

L

S

R

R

L

L

Q

Q

R

D

E

R

I

L

R

G

K

T

T

T

V

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

L

Q

D

T

E

E

A

A

I

M

R

T

L

L

G

G

D

D

R

R

I

V

A

V

V

V

F

M

R

-

Q

L

G

A

G

G

H

E

L

V

E

Q

Q

Q

I

I

D

G

P

T

P

P

A

D

S

E

S

L

A

-

R

-

P

-

T

Y

S

S

P

P

A

A

S

N

P

L

V

F

P

V

T

A

G

G

R

F

C

I

G

G

G

S

binding adenosine-5'-triphosphate: Y12 (= Y23), S38 (= S49), C40 (= C51), G41 (= G52), K42 (= K53), S43 (≠ T54), T44 (= T55), Q82 (= Q95), R129 (= R144), Q133 (≠ E148), S135 (= S150), G136 (= G151), G137 (= G152), Q159 (≠ E174), H192 (= H207)
binding magnesium ion: S43 (≠ T54), Q82 (= Q95)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
41% identity, 92% coverage: 14:250/259 of query aligns to 7:229/353 of 1vciA

query
sites
1vciA

I

V

S

K

L

L

R

E

G

N

V

L

G

T

K

K

R

R

Y
x
F

P

G

D

N

G
x
F

T

T

V

-

A

A

V

V

A

N

D

K

L

L

S

N

L

L

D

T

V

I

P

K

A

D

G

G

E

E

L

F

L

L

C

V

L

L

V

L

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
|
T

T

T

M

L

K

R

M

M

I

I

N

A

R

G

L

L

V

E

E

E

P

P

T

T

S

E

G

G

H

R

I

I

L

Y

V

F

A

G

G

D

E

R

D

D

V

V

A

T

R

Y

T

L

D

P

P

P

V

K

R

D

L

-

R

-

R

R

R

N

I

I

G

S

Y

M

V

V

I

-

Q

-

I

-

G

-

L

-

F

F

P

Q

H

H

L

M

T

T

V

V

H

Y

A

E

N

N

V

I

G

A

T

F

V

P

P

L

R

K

M

K

L

F

G

P

W

K

D

D

R

E

A

I

R

D

I

K

R

R

A

V

R

R

V

W

D

A

A

A

M

E

L

L

D

-

L

-

V

-

G

-

L

L

D

Q

P

I

A

E

T

E

M

L

G

L

R

N

R

R

Y

Y

P

P

H

A

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

G

A

V

V

A

A

R

R

A

A

L

I

A

V

D

E

P

P

P

D

V

V

L

L

M

M

D

D

E

E

P

P

F

L

S

S

A

N

I

L

D

D

P

A

I

K

A

L

R

R

D

V

R

A

L

M

Q

R

A

A

E

E

F

I

L

K

R

K

L

L

Q

Q

R

Q

E

K

I

L

R

K

K

V

T

T

V

T

V

I

F

Y

V

V

T

T

H

H

D

D

L

Q

D

V

E

E

A

A

I

M

R

T

L

M

G

G

D

D

R

R

I

I

A

A

V

V

F

M

R

N

Q

R

G

-

G

G

H

Q

L

L

E

L

Q

Q

I

I

D

G

P

S

P

P

A

T

S

E

S

V

A

Y

A

L

R

R

P

P

P

N

T

S

S

V

S

F

P

V

A

A

S

T

P

F

V

I

binding adenosine-5'-triphosphate: F16 (≠ Y23), F19 (≠ G26), S41 (= S49), G42 (= G50), G44 (= G52), K45 (= K53), T46 (= T54), T47 (= T55)

8hplC Lpqy-sugabc in state 1 (see paper)
40% identity, 94% coverage: 14:256/259 of query aligns to 3:233/384 of 8hplC

query
sites
8hplC

I

I

S

V

L

L

R

D

G

R

V

V

G

T

K

K

R

S

Y
|
Y

P

P

D

R

G

A

T

-

V

-

A

A

V

V

A

K

D

E

L

F

S

S

L

M

D

T

V

I

P

A

A

D

G

G

E

E

L

F

L

I

C

I

L

L

V

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

T

M

L

K

N

M

M

I

I

N

A

R

G

L

L

V

E

E

E

P

I

T

T

S

S

G

G

H

E

I

L

L

R

V

I

A

G

G

G

E

E

D

R

V

V

A

N

R

E

T

K

D

A

P

P

V

K

R

D

L

-

R

-

R

R

R

D

I

I

G

A

Y

M

V

V

I

F

Q

Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

H

H

L

M

T

T

V

V

H

R

A

Q

N

N

V

I

G

A

T

F

V

P

P

L

R

T

M

L

L

A

G

K

W

V

D

P

R

K

A

A

R

E

I

I

R

A

A

A

R

K

V

V

D

E

A

E

M

T

L

A

D

K

L

I

V

-

G

-

L

L

D

D

P

L

A

S

T

E

M

L

G

L

R

D

R

R

Y

K

P

P

H

G

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

G

A

V

M

A

G

R

R

A

A

L

I

A

V

A

R

D

S

P

P

P

K

V

A

L

F

L

L

M

M

D

D

E

Q

P

P

F

L

S

S

A

N

I

L

D

D

P

A

I

K

A

L

R

R

D

V

R

Q

L

M

Q

R

A

A

E

E

F

I

L

S

R

R

L

L

Q

Q

R

D

E

R

I

L

R

G

K

T

T

T

V

T

V

V

F

Y

V

V

T

T

H

H

D

D

L

Q

D

T

E

E

A

A

I

M

R

T

L

L

G

G

D

D

R

R

I

V

A

V

V

V

F

M

R

L

Q

-

G

A

G

G

H

E

L

V

E

Q

Q

Q

I

I

D

G

P

T

P

P

A

D

S

E

S

-

A

-

R

-

P

-

P

L

T

Y

S

S

P

P

A

A

S

N

P

L

V

F

P

V

T

A

G

G

R

F

C

I

G

G

binding adenosine-5'-triphosphate: Y12 (= Y23), G37 (= G50), C38 (= C51), G39 (= G52), K40 (= K53), S41 (≠ T54), T42 (= T55)

3fvqB Crystal structure of the nucleotide binding domain fbpc complexed with atp (see paper)
43% identity, 80% coverage: 26:233/259 of query aligns to 15:222/350 of 3fvqB

query
sites
3fvqB

G

N

T
|
T

V

P

A
x
V

V

L

A

N

D

D

L

I

S

S

L

L

D

S

V

L

P

D

A

P

G

G

E

E

L

I

L

L

C

F

L

I

V

I

G

G

P

A

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

T

L

M

L

K

R

M

C

I

L

N

A

R

G

L

F

V

E

E

Q

P

P

T

D

S

S

G

G

H

E

I

I

L

S

V

L

A

S

G

G

E

K

D

T

V

I

A

F

R

S

T

K

D

N

-

T

-

N

-

L

P

P

V

V

R

R

L

-

R

E

R

R

I

L

G

G

Y

Y

V

L

I

V

Q
|
Q

Q

E

I

G

G

V

L

L

F

F

P

P

H

H

L

L

T

T

V

V

H

Y

A

R

N

N

V

I

G

A

T

Y

V

-

P

-

R

-

M

G

L

L

G

G

W

N

D

G

R

K

A

G

R

R

I

T

-

A

-

Q

-

E

R

R

A

Q

R

R

V

I

D

E

A

A

M

M

L

L

D

E

L

L

V

T

G

G

L

I

D

-

P

-

A

S

T

E

M

L

G

A

R

G

R
|
R

Y

Y

P

P

H

H

E
|
E

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

Q

R

R

V

A

G

A

V

L

A

A

R

R

A

A

L

L

A

A

A

P

D

D

P

P

P

E

V

L

L

I

L

L

M

L

D

D

E

E

P

P

F

F

S

S

A

A

I

L

D

D

P

E

I

Q

A

L

R

R

D

R

R

Q

L

I

Q

R

A

E

E

D

F

M

L

I

R

A

L

A

Q

L

R

R

E

A

I

N

R

G

K

K

T

S

V

A

V

V

F

F

V

V

T

S

H

H

D

D

L

R

D

E

E

E

A

A

I

L

R

Q

L

Y

G

A

D

D

R

R

I

I

A

A

V

V

F

M

R

K

Q

Q

G

G

G

R

H

I

L

L

E

Q

Q

T

I

A

D

S

P

P

binding adenosine-5'-triphosphate: T16 (= T27), V18 (≠ A29), S38 (= S49), G39 (= G50), C40 (= C51), G41 (= G52), K42 (= K53), T43 (= T54), T44 (= T55), R133 (= R144), E137 (= E148), S139 (= S150), G141 (= G152), Q142 (= Q153)
binding calcium ion: T43 (= T54), Q86 (= Q95)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13, 14

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
39% identity, 92% coverage: 12:248/259 of query aligns to 1:234/241 of 4u00A

query
sites
4u00A

P

P

M

I

I

I

S

R

L

I

R

R

G

N

V

L

G

H

K

K

R

W

Y
x
F

P

G

D

P

G

L

T

H

V
|
V

A

-

V

L

A

K

D

G

L

I

S

H

L

L

D

E

V

V

P

A

A

P

G

G

E

E

L

K

L

L

C

V

L

I

V

I

G

G

P

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

T
x
S

T
|
T

T

L

M

I

K

R

M

T

I

I

N

N

R

R

L

L

V

E

E

D

P

F

T

Q

S

E

G

G

H

E

I

V

L

V

V

V

A

D

G

G

E

L

D

S

V

V

A

K

R

D

T

D

D

R

P

A

V

L

R

R

-

E

L

I

R

R

R

E

I

V

G

G

Y

M

V

V

I

F

Q

Q

I

F

G

N

L

L

F

F

P

P

H

H

L

M

T

T

V

V

H

L

A

E

N

N

V

V

G

T

T

L

V

A

P

P

-

M

R

R

M

V

L

R

G

R

W

W

D

P

R

R

A

E

R

K

I

A

R

E

A

K

R

K

V

A

D

L

A

E

M

L

L

L

D

E

L

R

V

V

G

G

-

I

L

L

D

D

P

Q

A

A

T

-

M

-

G

-

R

R

R

K

Y

Y

P

P

H

A

E

Q

L

L

S

S

G

G

Q

Q

R

R

V

V

G

A

V

I

A

A

R

R

A

A

L

L

A

A

A

M

D

E

P

P

P

K

V

I

L

M

L

L

M

F

D

D

E

E

P

P

F

T

S

S

A

A

I

L

D

D

P

P

I

-

A

-

R

-

D

-

R

E

L

M

Q

V

A

G

E

E

F

V

L

L

R

D

L

V

Q

M

R

R

E

D

I

L

R

A

K

Q

-

G

-

M

T

T

V

M

V

V

F

V

V

V

T

T

H

H

D

E

L

M

D

G

E

F

A

A

I

R

R

E

L

V

G

A

D

D

R

R

I

V

A

-

V

V

F

F

R

M

Q

D

G

G

H

Q

L

I

E

V

Q

E

I

E

D

G

P

R

P

P

A

E

S

E

S

I

A

F

A

T

R

R

P

P

P

K

T

E

S

E

S

R

P

T

A

R

S

S

binding adenosine-5'-diphosphate: F12 (≠ Y23), V17 (= V28), S37 (= S49), G38 (= G50), S39 (≠ C51), G40 (= G52), K41 (= K53), S42 (≠ T54), T43 (= T55)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
36% identity, 88% coverage: 14:241/259 of query aligns to 3:224/371 of 3puyA

query
sites
3puyA

I

V

S

Q

L

L

R

Q

G

N

V

V

G

T

K

K

R

A

Y
x
W

P

G

D

E

G

V

T

V

V

V

A

S

V

-

A

K

D

D

L

I

S

N

L

L

D

D

V

I

P

H

A

E

G

G

E

E

L

F

L

V

C

V

L

F

V

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

M

L

K

R

M

M

I

I

N

A

R

G

L

L

V

E

E

T

P

I

T

T

S

S

G

G

H

D

I

L

L

F

V

I

A

G

G

E

E

K

D

R

V

M

A

N

R

D

T

T

D

P

P

P

V

A

R

E

L

-

R

-

R

R

R

G

I

V

G

G

Y

M

V

V

I

F

Q
|
Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

H

A

A

E

N

N

V

M

G

S

T

F

V

G

P

L

R

K

M

L

L

A

G

G

W

A

D

K

R

K

A

E

R

V

I

I

R

N

A

Q

R

R

V

V

D

N

A

Q

M

V

L

A

D

E

L

V

V

L

G

Q

L

L

D

-

P

-

A

A

T

H

M

L

G

L

R

D

R
|
R

Y

K

P

P

H

K

E
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

G

A

V

I

A

G

R

R

A

T

L

L

A

V

A

A

D

E

P

P

P

S

V

V

L

F

L

L

M

L

D

D

E
|
E

P

P

F

L

S

S

A

N

I

L

D

D

P

A

I

A

A

L

R

R

D

V

R

Q

L

M

Q

R

A

I

E

E

F

I

L

S

R

R

L

L

Q

H

R

K

E

R

I

L

R

G

K

R

T

T

V

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

L

Q

D

V

E

E

A

A

I

M

R

T

L

L

G

A

D

D

R

K

I

I

A

V

V

V

F

L

R

-

Q

D

G

A

G

G

H

R

L

V

E

A

Q

Q

I

V

D

G

P

K

P

P

A

L

S

E

S

L

A

Y

A

H

R

Y

P

P

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y23), S37 (= S49), G38 (= G50), C39 (= C51), G40 (= G52), K41 (= K53), S42 (≠ T54), T43 (= T55), Q81 (= Q95), R128 (= R144), A132 (≠ E148), S134 (= S150), G136 (= G152), Q137 (= Q153), E158 (= E174), H191 (= H207)
binding magnesium ion: S42 (≠ T54), Q81 (= Q95)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
36% identity, 88% coverage: 14:241/259 of query aligns to 3:224/371 of 3puxA

query
sites
3puxA

I

V

S

Q

L

L

R

Q

G

N

V

V

G

T

K

K

R

A

Y
x
W

P

G

D

E

G

V

T

V

V

V

A

S

V

-

A

K

D

D

L

I

S

N

L

L

D

D

V

I

P

H

A

E

G

G

E

E

L

F

L

V

C

V

L

F

V

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

M

L

K

R

M

M

I

I

N

A

R

G

L

L

V

E

E

T

P

I

T

T

S

S

G

G

H

D

I

L

L

F

V

I

A

G

G

E

E

K

D

R

V

M

A

N

R

D

T

T

D

P

P

P

V

A

R

E

L

-

R

-

R

R

R

G

I

V

G

G

Y

M

V

V

I

F

Q
|
Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

H

A

A

E

N

N

V

M

G

S

T

F

V

G

P

L

R

K

M

L

L

A

G

G

W

A

D

K

R

K

A

E

R

V

I

I

R

N

A

Q

R

R

V

V

D

N

A

Q

M

V

L

A

D

E

L

V

V

L

G

Q

L

L

D

-

P

-

A

A

T

H

M

L

G

L

R

D

R
|
R

Y

K

P

P

H

K

E

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

G

A

V

I

A

G

R

R

A

T

L

L

A

V

A

A

D

E

P

P

P

S

V

V

L

F

L

L

M

L

D

D

E

E

P

P

F

L

S

S

A

N

I

L

D

D

P

A

I

A

A

L

R

R

D

V

R

Q

L

M

Q

R

A

I

E

E

F

I

L

S

R

R

L

L

Q

H

R

K

E

R

I

L

R

G

K

R

T

T

V

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

L

Q

D

V

E

E

A

A

I

M

R

T

L

L

G

A

D

D

R

K

I

I

A

V

V

V

F

L

R

-

Q

D

G

A

G

G

H

R

L

V

E

A

Q

Q

I

V

D

G

P

K

P

P

A

L

S

E

S

L

A

Y

A

H

R

Y

P

P

binding adenosine-5'-diphosphate: W12 (≠ Y23), G38 (= G50), C39 (= C51), G40 (= G52), K41 (= K53), S42 (≠ T54), T43 (= T55), R128 (= R144), S134 (= S150), Q137 (= Q153)
binding beryllium trifluoride ion: S37 (= S49), G38 (= G50), K41 (= K53), Q81 (= Q95), S134 (= S150), G136 (= G152), H191 (= H207)
binding magnesium ion: S42 (≠ T54), Q81 (= Q95)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
36% identity, 88% coverage: 14:241/259 of query aligns to 3:224/371 of 3puwA

query
sites
3puwA

I

V

S

Q

L

L

R

Q

G

N

V

V

G

T

K

K

R

A

Y
x
W

P

G

D

E

G

V

T

V

V
|
V

A

S

V

-

A

K

D

D

L

I

S

N

L

L

D

D

V

I

P

H

A

E

G

G

E

E

L

F

L

V

C

V

L

F

V

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

M

L

K

R

M

M

I

I

N

A

R

G

L

L

V

E

E

T

P

I

T

T

S

S

G

G

H

D

I

L

L

F

V

I

A

G

G

E

E

K

D

R

V

M

A

N

R

D

T

T

D

P

P

P

V

A

R

E

L

-

R

-

R

R

R

G

I

V

G

G

Y

M

V

V

I

F

Q
|
Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

H

A

A

E

N

N

V

M

G

S

T

F

V

G

P

L

R

K

M

L

L

A

G

G

W

A

D

K

R

K

A

E

R

V

I

I

R

N

A

Q

R

R

V

V

D

N

A

Q

M

V

L

A

D

E

L

V

V

L

G

Q

L

L

D

-

P

-

A

A

T

H

M

L

G

L

R

D

R
|
R

Y

K

P

P

H

K

E
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

G

A

V

I

A

G

R

R

A

T

L

L

A

V

A

A

D

E

P

P

P

S

V

V

L

F

L

L

M

L

D

D

E
|
E

P

P

F

L

S

S

A

N

I

L

D

D

P

A

I

A

A

L

R

R

D

V

R

Q

L

M

Q

R

A

I

E

E

F

I

L

S

R

R

L

L

Q

H

R

K

E

R

I

L

R

G

K

R

T

T

V

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

L

Q

D

V

E

E

A

A

I

M

R

T

L

L

G

A

D

D

R

K

I

I

A

V

V

V

F

L

R

-

Q

D

G

A

G

G

H

R

L

V

E

A

Q

Q

I

V

D

G

P

K

P

P

A

L

S

E

S

L

A

Y

A

H

R

Y

P

P

binding adenosine-5'-diphosphate: W12 (≠ Y23), V17 (= V28), G38 (= G50), C39 (= C51), G40 (= G52), K41 (= K53), S42 (≠ T54), T43 (= T55), R128 (= R144), A132 (≠ E148), S134 (= S150), Q137 (= Q153)
binding tetrafluoroaluminate ion: S37 (= S49), G38 (= G50), K41 (= K53), Q81 (= Q95), S134 (= S150), G135 (= G151), G136 (= G152), E158 (= E174), H191 (= H207)
binding magnesium ion: S42 (≠ T54), Q81 (= Q95)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
36% identity, 88% coverage: 14:241/259 of query aligns to 3:224/371 of 3puvA

query
sites
3puvA

I

V

S

Q

L

L

R

Q

G

N

V

V

G

T

K

K

R

A

Y
x
W

P

G

D

E

G

V

T

V

V
|
V

A

S

V

-

A

K

D

D

L

I

S

N

L

L

D

D

V

I

P

H

A

E

G

G

E

E

L

F

L

V

C

V

L

F

V

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

M

L

K

R

M

M

I

I

N

A

R

G

L

L

V

E

E

T

P

I

T

T

S

S

G

G

H

D

I

L

L

F

V

I

A

G

G

E

E

K

D

R

V

M

A

N

R

D

T

T

D

P

P

P

V

A

R

E

L

-

R

-

R

R

R

G

I

V

G

G

Y

M

V

V

I

F

Q
|
Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

H

A

A

E

N

N

V

M

G

S

T

F

V

G

P

L

R

K

M

L

L

A

G

G

W

A

D

K

R

K

A

E

R

V

I

I

R

N

A

Q

R

R

V

V

D

N

A

Q

M

V

L

A

D

E

L

V

V

L

G

Q

L

L

D

-

P

-

A

A

T

H

M

L

G

L

R

D

R
|
R

Y

K

P

P

H

K

E
x
A

L

L

S
|
S

G

G

Q
|
Q

Q

R

Q

Q

R

R

V

V

G

A

V

I

A

G

R

R

A

T

L

L

A

V

A

A

D

E

P

P

P

S

V

V

L

F

L

L

M

L

D

D

E

E

P

P

F

L

S

S

A

N

I

L

D

D

P

A

I

A

A

L

R

R

D

V

R

Q

L

M

Q

R

A

I

E

E

F

I

L

S

R

R

L

L

Q

H

R

K

E

R

I

L

R

G

K

R

T

T

V

M

V

I

F

Y

V

V

T

T

H

H

D

D

L

Q

D

V

E

E

A

A

I

M

R

T

L

L

G

A

D

D

R

K

I

I

A

V

V

V

F

L

R

-

Q

D

G

A

G

G

H

R

L

V

E

A

Q

Q

I

V

D

G

P

K

P

P

A

L

S

E

S

L

A

Y

A

H

R

Y

P

P

binding adenosine-5'-diphosphate: W12 (≠ Y23), V17 (= V28), G38 (= G50), C39 (= C51), G40 (= G52), K41 (= K53), S42 (≠ T54), T43 (= T55), R128 (= R144), A132 (≠ E148), S134 (= S150), Q137 (= Q153)
binding magnesium ion: S42 (≠ T54), Q81 (= Q95)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
36% identity, 88% coverage: 14:241/259 of query aligns to 4:225/371 of P68187

query
sites
P68187

I

V

S

Q

L

L

R

Q

G

N

V

V

G

T

K

K

R

A

Y

W

P

G

D

E

G

V

T

V

V

V

A

S

V

-

A

K

D

D

L

I

S

N

L

L

D

D

V

I

P

H

A

E

G

G

E

E

L

F

L

V

C

V

L

F

V

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

T

L

M

L

K

R

M

M

I

I

N

A

R

G

L

L

V

E

E

T

P

I

T

T

S

S

G

G

H

D

I

L

L

F

V

I

A

G

G

E

E

K

D

R

V

M

A

N

R

D

T

T

D

P

P

P

V

A

R

E

L

-

R

-

R

R

R

G

I

V

G

G

Y

M

V

V

I

F

Q

Q

Q

S

I

Y

G
x
A

L

L

F

Y

P

P

H

H

L

L

T

S

V

V

H

A

A

E

N

N

V

M

G

S

T

F

V

G

P

L

R

K

M

L

L

A

G

G

W

A

D
x
K

R

K

A

E

R

V

I

I

R

N

A

Q

R

R

V
|
V

D

N

A

Q

M
x
V

L

A

D
x
E

L

V

V

L

G

Q

L

L

D

-

P

-

A
|
A

T

H

M

L

G

L

R

D

R

R

Y

K

P

P

H

K

E

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

G

A

V

I

A

G

R

R

A

T

L

L

A

V

A

A

D

E

P

P

P

S

V

V

L

F

L

L

M

L

D
|
D

E

E

P

P

F

L

S

S

A

N

I

L

D

D

P

A

I

A

A

L

R

R

D

V

R

Q

L

M

Q

R

A

I

E

E

F

I

L

S

R

R

L

L

Q

H

R

K

E

R

I

L

R

G

K

R

T

T

V

M

V

I

F

Y

V

V

T

T

H

H

D

D

L

Q

D

V

E

E

A

A

I

M

R

T

L

L

G

A

D

D

R

K

I

I

A

V

V

V

F

L

R

-

Q

D

G

A

G

G

H

R

L

V

E

A

Q

Q

I

V

D

G

P

K

P

P

A

L

S

E

S

L

A

Y

A

H

R

Y

P

P

A85 (≠ G98) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ D119) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V127) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ M130) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ D132) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (= A139) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G152) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D173) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
37% identity, 85% coverage: 14:234/259 of query aligns to 3:217/374 of 2awnB

query
sites
2awnB

I

V

S

Q

L

L

R

Q

G

N

V

V

G

T

K

K

R

A

Y
x
W

P

G

D

E

G

V

T

V

V
|
V

A

S

V

-

A

K

D

D

L

I

S

N

L

L

D

D

V

I

P

H

A

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S

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binding adenosine-5'-diphosphate: W12 (≠ Y23), V17 (= V28), S37 (= S49), G38 (= G50), G40 (= G52), K41 (= K53), S42 (≠ T54), T43 (= T55)

Query Sequence

>WP_308205971.1 NCBI__GCF_000058485.1:WP_308205971.1
MTTVESTTAADPMISLRGVGKRYPDGTVAVADLSLDVPAGELLCLVGPSGCGKTTTMKMI
NRLVEPTSGHILVAGEDVARTDPVRLRRRIGYVIQQIGLFPHLTVHANVGTVPRMLGWDR
ARIRARVDAMLDLVGLDPATMGRRYPHELSGGQQQRVGVARALAADPPVLLMDEPFSAID
PIARDRLQAEFLRLQREIRKTVVFVTHDLDEAIRLGDRIAVFRQGGHLEQIDPPASSAAR
PPTSSPASPVPTGRCGGCR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory