SitesBLAST
Comparing WP_317623054.1 NCBI__GCF_900102855.1:WP_317623054.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P05041 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Escherichia coli (strain K12) (see 4 papers)
48% identity, 95% coverage: 12:454/464 of query aligns to 11:452/453 of P05041
- S36 (= S36) binding L-tryptophan
- E258 (= E260) mutation to A: The reaction is extremely slow.; mutation to D: The reaction is extremely slow.
- K274 (= K276) mutation to A: Absence of covalent intermediate. Addition of ammonia allows the formation of the covalent intermediate and shows that ammonia can replace the function of K-274. Reduced catalytic efficiency.; mutation to R: Absence of covalent intermediate.; mutation to R: Reduced catalytic efficiency.
- G275 (= G277) mutation to S: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
- R311 (= R313) mutation to K: Catalytically active in the NH3-dependent, but inactive for the glutamine-dependent reactions and fails to complex with PabA.
- R316 (≠ K318) mutation to H: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
- S322 (= S324) mutation to T: Complete loss of aminodeoxychorismate synthase activity.
- H339 (= H341) mutation to W: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
1k0eA The crystal structure of aminodeoxychorismate synthase from formate grown crystals (see paper)
47% identity, 95% coverage: 12:454/464 of query aligns to 9:436/437 of 1k0eA
- active site: E256 (= E260), K272 (= K276), E286 (= E304), H323 (= H341), S350 (= S368), W374 (≠ Y392), R394 (= R412), G410 (= G428), E423 (= E441), K427 (= K445)
- binding tryptophan: L32 (= L34), H33 (≠ D35), S34 (= S36), Y41 (vs. gap), F44 (= F42), P238 (= P242), F239 (= F243), S240 (≠ A244)
1k0gA The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
45% identity, 95% coverage: 12:454/464 of query aligns to 11:419/420 of 1k0gA
- active site: E258 (= E260), K274 (= K276), E278 (= E304), S333 (= S368), W357 (≠ Y392), R377 (= R412), G393 (= G428), E406 (= E441), K410 (= K445)
- binding phosphate ion: D113 (= D108), R116 (≠ C111), D347 (≠ E382), R353 (≠ P388)
- binding tryptophan: L34 (= L34), H35 (≠ D35), S36 (= S36), Y43 (vs. gap), S44 (≠ G40), F46 (= F42), P240 (= P242), F241 (= F243), S242 (≠ A244)
1k0gB The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
44% identity, 95% coverage: 12:453/464 of query aligns to 11:415/415 of 1k0gB
- active site: E258 (= E260), K274 (= K276), E277 (= E304), S330 (= S368), W354 (≠ Y392), R374 (= R412), G390 (= G428), E403 (= E441), K407 (= K445)
- binding phosphate ion: Y112 (= Y107), D113 (= D108), R116 (≠ C111), D344 (≠ E382), R350 (≠ P388)
- binding tryptophan: L34 (= L34), H35 (≠ D35), S36 (= S36), Y43 (vs. gap), S44 (≠ G40), R45 (= R41), F46 (= F42), P240 (= P242), F241 (= F243)
7pi1DDD Aminodeoxychorismate synthase component 1
37% identity, 96% coverage: 10:454/464 of query aligns to 7:454/459 of 7pi1DDD
- binding magnesium ion: G428 (= G428), E438 (= E438)
- binding tryptophan: L33 (= L34), E34 (≠ D35), S35 (= S36), G39 (= G40), Y41 (≠ F42), P242 (= P242), Y243 (≠ F243), M244 (≠ A244), Q406 (≠ D406), N408 (= N408)
P28820 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Bacillus subtilis (strain 168) (see paper)
37% identity, 96% coverage: 10:454/464 of query aligns to 9:461/470 of P28820
- A283 (≠ K276) mutation to I: Complete loss of aminodeoxychorismate synthase activity.; mutation to K: Absence of covalent intermediate.; mutation to V: Complete loss of aminodeoxychorismate synthase activity.
8hx9A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae with chorismate (see paper)
40% identity, 77% coverage: 97:454/464 of query aligns to 263:632/632 of 8hx9A
- binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: I453 (= I275), K454 (= K276), G455 (= G277), T456 (= T278), M547 (≠ I369), Y570 (= Y392), R590 (= R412), V603 (≠ A425), G604 (= G426), G605 (= G427), A606 (≠ G428), E619 (= E441), K623 (= K445)
- binding tryptophan: P419 (= P242), Y420 (≠ F243), G421 (≠ A244), L574 (≠ I396), G575 (= G397)
Sites not aligning to the query:
8hx8A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae co-crystallized with chorismate (see paper)
40% identity, 77% coverage: 97:455/464 of query aligns to 305:672/673 of 8hx8A
Sites not aligning to the query:
O94582 Probable anthranilate synthase component 1; Anthranilate synthase component I; EC 4.1.3.27 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
32% identity, 90% coverage: 28:445/464 of query aligns to 46:466/489 of O94582
- S390 (≠ T370) modified: Phosphoserine
- S392 (≠ A372) modified: Phosphoserine
Sites not aligning to the query:
- 488 modified: Phosphoserine
P32068 Anthranilate synthase alpha subunit 1, chloroplastic; Anthranilate synthase component 1-1; Anthranilate synthase component I-1; Protein A-METHYL TRYPTOPHAN RESISTANT 1; Protein JASMONATE-INDUCED DEFECTIVE LATERAL ROOT 1; Protein TRYPTOPHAN BIOSYNTHESIS 5; Protein WEAK ETHYLENE INSENSITIVE 2; EC 4.1.3.27 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 89% coverage: 37:449/464 of query aligns to 123:581/595 of P32068
- D341 (= D227) mutation to N: In trp5-1; insensitive to feedback inhibition by tryptophan and resistance to the herbicide 6-methylanthranilate.
Q94GF1 Anthranilate synthase alpha subunit 1, chloroplastic; OsASA1; EC 4.1.3.27 from Oryza sativa subsp. japonica (Rice) (see paper)
30% identity, 89% coverage: 35:449/464 of query aligns to 105:563/577 of Q94GF1