SitesBLAST
Comparing YP_001314493.1 NCBI__GCF_000017145.1:YP_001314493.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3bjsB Crystal structure of a member of enolase superfamily from polaromonas sp. Js666
35% identity, 91% coverage: 32:385/389 of query aligns to 29:366/376 of 3bjsB
- active site: P49 (≠ S56), S52 (≠ T59), L93 (≠ A100), G136 (≠ S155), K164 (= K181), R166 (= R183), D194 (= D214), N196 (≠ H216), E220 (= E240), G246 (= G265), E247 (= E266), N248 (≠ S267), Q268 (= Q287), D270 (= D289), H297 (= H316), S298 (≠ L317), S299 (≠ W318), E322 (= E341), C324 (≠ S343), K327 (≠ A346)
- binding magnesium ion: D194 (= D214), E220 (= E240), E247 (= E266)
Sites not aligning to the query:
4hpnA Crystal structure of a proposed galactarolactone cycloisomerase from agrobacterium tumefaciens, target efi-500704, with bound ca, ordered loops (see paper)
32% identity, 95% coverage: 13:382/389 of query aligns to 1:370/378 of 4hpnA
- active site: F19 (= F31), G50 (= G55), R53 (≠ G58), T134 (≠ S155), K164 (= K181), K166 (≠ R183), D194 (= D214), N196 (≠ H216), E220 (= E240), G245 (= G265), E246 (= E266), T247 (≠ S267), Q267 (= Q287), D269 (= D289), H296 (= H316), V297 (≠ L317), W298 (= W318), R320 (vs. gap), E329 (= E341), F330 (≠ Y342), H334 (≠ A346)
- binding calcium ion: D194 (= D214), D209 (≠ Q229), E220 (= E240), G237 (≠ A257), E246 (= E266)
3sjnA Crystal structure of enolase spea_3858 (target efi-500646) from shewanella pealeana with magnesium bound
30% identity, 99% coverage: 1:385/389 of query aligns to 2:370/373 of 3sjnA
- active site: S46 (≠ N52), L49 (vs. gap), T139 (≠ G157), K165 (= K181), G167 (≠ R183), M171 (= M187), D198 (= D214), A200 (= A215), E225 (= E240), I247 (= I262), G250 (= G265), E251 (= E266), S252 (= S267), Q272 (= Q287), D274 (= D289), H301 (= H316), G302 (≠ L317), F303 (≠ W318), M325 (≠ V340), E326 (= E341), Q329 (≠ L344), S331 (≠ A346)
- binding magnesium ion: D198 (= D214), E225 (= E240), E251 (= E266)
3ozmA Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
32% identity, 99% coverage: 1:386/389 of query aligns to 2:373/386 of 3ozmA
- active site: M20 (≠ R19), G53 (= G58), D56 (vs. gap), S143 (= S155), K170 (= K181), K172 (≠ R183), D200 (= D214), N202 (≠ H216), E226 (= E240), G252 (= G265), E253 (= E266), N254 (≠ S267), Q274 (= Q287), D276 (= D289), H303 (= H316), T304 (≠ L317), F305 (≠ W318), E328 (= E341), I331 (≠ L344), H333 (≠ A346)
- binding D-xylaric acid: S24 (= S23), K29 (≠ L28), Y146 (≠ G157), K170 (= K181), K172 (≠ R183), D200 (= D214), N202 (≠ H216), E253 (= E266), H303 (= H316), F305 (≠ W318), E328 (= E341)
- binding magnesium ion: D200 (= D214), E226 (= E240), E253 (= E266)
3ozmD Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
32% identity, 99% coverage: 1:386/389 of query aligns to 2:373/381 of 3ozmD
- active site: M20 (≠ R19), G53 (= G58), D56 (vs. gap), S143 (= S155), K170 (= K181), K172 (≠ R183), D200 (= D214), N202 (≠ H216), E226 (= E240), G252 (= G265), E253 (= E266), N254 (≠ S267), Q274 (= Q287), D276 (= D289), H303 (= H316), T304 (≠ L317), F305 (≠ W318), E328 (= E341), I331 (≠ L344), H333 (≠ A346)
- binding L-arabinaric acid: K172 (≠ R183), D200 (= D214), N202 (≠ H216), E253 (= E266), H303 (= H316), F305 (≠ W318), E328 (= E341)
- binding magnesium ion: D200 (= D214), E226 (= E240), E253 (= E266)
5olcC Crystal structure of the 3,6-anhydro-d-galactonate cycloisomerase from zobellia galactanivorans (see paper)
31% identity, 92% coverage: 30:388/389 of query aligns to 18:351/351 of 5olcC
- active site: K148 (= K181), K150 (≠ R183), D178 (= D214), N180 (≠ H216), E204 (= E240), G229 (= G265), E230 (= E266), D253 (= D289), H280 (= H316), E304 (= E341), E309 (≠ A346)
- binding magnesium ion: D178 (= D214), E204 (= E240), E230 (= E266)
3op2A Crystal structure of putative mandelate racemase from bordetella bronchiseptica rb50 complexed with 2-oxoglutarate/phosphate
31% identity, 100% coverage: 1:388/389 of query aligns to 2:370/375 of 3op2A
- active site: M20 (≠ R19), G53 (= G58), D56 (vs. gap), S138 (= S155), K165 (= K181), K167 (≠ R183), D195 (= D214), N197 (≠ H216), E221 (= E240), G247 (= G265), E248 (= E266), N249 (≠ S267), Q269 (= Q287), D271 (= D289), H298 (= H316), T299 (≠ L317), F300 (≠ W318), E323 (= E341), I326 (≠ L344), H328 (≠ A346)
- binding 2-oxoglutaric acid: K165 (= K181), K167 (≠ R183), D195 (= D214), E248 (= E266), H298 (= H316), E323 (= E341)
- binding magnesium ion: D195 (= D214), E221 (= E240), E248 (= E266)
3stpA Crystal structure of a putative galactonate dehydratase
33% identity, 89% coverage: 37:384/389 of query aligns to 51:385/390 of 3stpA
- active site: L66 (≠ G55), S69 (≠ G58), S151 (= S155), K177 (= K181), R179 (= R183), P189 (= P191), E214 (≠ D214), Y216 (≠ H216), E240 (= E240), G265 (= G265), E266 (= E266), H267 (≠ S267), Q287 (= Q287), D289 (= D289), I311 (≠ L311), H316 (= H316), E336 (≠ P335), F341 (≠ V340)
- binding magnesium ion: E214 (≠ D214), E240 (= E240), E266 (= E266)
2qq6B Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from rubrobacter xylanophilus dsm 9941
32% identity, 87% coverage: 46:385/389 of query aligns to 31:386/396 of 2qq6B
- active site: P37 (≠ N52), G79 (= G103), D124 (≠ S155), K166 (= K181), D168 (≠ R183), D213 (= D214), H215 (= H216), E239 (= E240), G264 (= G265), E265 (= E266), M286 (≠ Q287), D288 (= D289), H315 (= H316), N316 (≠ L317), E340 (= E341), D345 (≠ A346)
- binding magnesium ion: D213 (= D214), E239 (= E240), E265 (= E266), H315 (= H316)
3sqsA Crystal structure of a putative mandelate racemase/muconate lactonizing protein from dinoroseobacter shibae dfl 12
32% identity, 89% coverage: 37:384/389 of query aligns to 47:381/386 of 3sqsA
- active site: L62 (≠ G55), N65 (≠ G58), S147 (= S155), K173 (= K181), R175 (= R183), G177 (= G185), G179 (≠ M187), K181 (vs. gap), A185 (= A189), E210 (≠ D214), Y212 (≠ H216), E236 (= E240), G261 (= G265), E262 (= E266), H263 (≠ S267), Q283 (= Q287), D285 (= D289), I307 (≠ L311), H312 (= H316), G314 (≠ W318), E332 (≠ P335), F337 (≠ V340)
- binding magnesium ion: E210 (≠ D214), E236 (= E240), E262 (= E266), Y347 (≠ H351), F350 (≠ V354), D351 (≠ E355)
3rraB Crystal structure of enolase prk14017 (target efi-500653) from ralstonia pickettii 12j with magnesium bound
33% identity, 91% coverage: 27:379/389 of query aligns to 6:349/379 of 3rraB
- active site: I35 (≠ A53), R38 (≠ S56), Y118 (= Y153), K145 (= K181), N147 (vs. gap), E151 (≠ R183), D184 (= D214), H186 (= H216), E210 (= E240), G235 (= G265), E236 (= E266), R237 (≠ S267), Q257 (= Q287), D259 (= D289), H286 (= H316), P288 (≠ W318), E311 (= E341)
- binding magnesium ion: D184 (= D214), E210 (= E240), E236 (= E266)
Q6BF17 D-galactonate dehydratase; GalD; EC 4.2.1.6 from Escherichia coli (strain K12)
31% identity, 90% coverage: 35:383/389 of query aligns to 17:359/382 of Q6BF17
- H185 (= H216) mutation H->N,Q: Loss of activity.
- H285 (= H316) mutation to N: Loss of activity.
- E310 (= E341) mutation to Q: Loss of activity.
4h83F Crystal structure of mandelate racemase/muconate lactonizing enzyme (efi target:502127)
30% identity, 96% coverage: 13:385/389 of query aligns to 10:365/368 of 4h83F
- active site: F20 (≠ H21), D51 (≠ N52), I139 (≠ S155), K166 (= K181), K168 (≠ R183), I196 (≠ V213), D197 (= D214), A198 (= A215), N199 (≠ H216), E223 (= E240), G249 (= G265), Q250 (≠ E266), N271 (≠ Q287), D273 (= D289), H300 (= H316), E301 (≠ G320), E302 (≠ A321), E320 (= E341), D325 (≠ G345)
- binding bicarbonate ion: R220 (≠ A237), I318 (≠ V339)
3no1A Crystal structure of mandelate racemase/muconate lactonizing enzyme from a marine actinobacterium in complex with magnesium
29% identity, 96% coverage: 13:385/389 of query aligns to 9:353/355 of 3no1A
- active site: D39 (≠ N52), I127 (≠ S155), K154 (= K181), K156 (≠ R183), I184 (≠ V213), D185 (= D214), A186 (= A215), N187 (≠ H216), E211 (= E240), G237 (= G265), Q238 (≠ E266), N259 (≠ Q287), D261 (= D289), H288 (= H316), E289 (≠ G320), E290 (≠ A321), E308 (= E341), D313 (≠ G345)
- binding magnesium ion: D185 (= D214), E211 (= E240), Q238 (≠ E266)
3rcyF Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from roseovarius sp. Tm1035
31% identity, 89% coverage: 35:379/389 of query aligns to 25:371/397 of 3rcyF
- active site: K165 (= K181), D167 (vs. gap), R175 (= R183), G208 (≠ D214), H210 (= H216), E234 (= E240), G259 (= G265), E260 (= E266), Q281 (= Q287), A283 (≠ D289), H310 (= H316), A313 (= A319), L332 (≠ F338), E335 (= E341)
- binding magnesium ion: E234 (= E240), E260 (= E266)
- binding alpha-D-ribofuranose: R85 (vs. gap), P86 (= P107), P239 (≠ A245), A266 (≠ F272), E267 (≠ A273)
3cb3A Crystal structure of l-talarate dehydratase from polaromonas sp. Js666 complexed with mg and l-glucarate
33% identity, 96% coverage: 10:381/389 of query aligns to 6:360/373 of 3cb3A
- active site: K24 (≠ R19), S57 (≠ A54), T143 (≠ S155), K171 (= K181), K173 (≠ R183), D202 (= D214), N204 (≠ H216), E228 (= E240), G253 (= G265), E254 (= E266), M275 (≠ Q287), D277 (= D289), H304 (= H316), F305 (≠ L317), A306 (≠ C323), E324 (= E341)
- binding l-glucaric acid: K171 (= K181), K173 (≠ R183), D202 (= D214), E254 (= E266), H304 (= H316)
- binding magnesium ion: D202 (= D214), E228 (= E240), A243 (≠ R255), F246 (≠ G258), E254 (= E266)
4h19A Crystal structure of an enolase (mandelate racemase subgroup, target efi-502087) from agrobacterium tumefaciens, with bound mg and d- ribonohydroxamate, ordered loop
30% identity, 97% coverage: 1:376/389 of query aligns to 1:358/372 of 4h19A
- active site: I20 (≠ Q20), T51 (≠ K51), T143 (≠ S155), K172 (= K181), K174 (≠ R183), D203 (= D214), N205 (≠ H216), E229 (= E240), G254 (= G265), E255 (= E266), Q276 (= Q287), D278 (= D289), H305 (= H316), A306 (= A319), G307 (= G320), E327 (= E341)
- binding (2R,3R,4R)-N,2,3,4,5-pentakis(oxidanyl)pentanamide: D22 (≠ R22), H25 (≠ F25), H52 (≠ N52), K172 (= K181), K174 (≠ R183), D203 (= D214), N205 (≠ H216), E229 (= E240), E255 (= E266), H305 (= H316), E327 (= E341)
- binding calcium ion: D268 (≠ V279), H298 (≠ F309)
- binding magnesium ion: D203 (= D214), E229 (= E240), E255 (= E266)
2og9A Crystal structure of mandelate racemase/muconate lactonizing enzyme from polaromonas sp. Js666
33% identity, 90% coverage: 32:381/389 of query aligns to 24:349/363 of 2og9A
- active site: S46 (≠ A54), T132 (≠ S155), K160 (= K181), K162 (≠ R183), D191 (= D214), N193 (≠ H216), E217 (= E240), G242 (= G265), E243 (= E266), M264 (≠ Q287), D266 (= D289), H293 (= H316), F294 (≠ L317), A295 (≠ C323), E313 (= E341)
- binding calcium ion: A232 (≠ R255), F235 (≠ G258)
Q8ZL58 L-talarate/galactarate dehydratase; TalrD/GalrD; StTGD; EC 4.2.1.156; EC 4.2.1.42 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
32% identity, 91% coverage: 28:380/389 of query aligns to 57:383/398 of Q8ZL58
- KR 82:83 (≠ GS 55:56) binding substrate
- K195 (= K181) binding substrate
- K197 (≠ R183) active site, Proton acceptor; mutation to A: Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
- D226 (= D214) binding Mg(2+)
- N228 (≠ H216) binding substrate
- E252 (= E240) binding Mg(2+)
- E278 (= E266) binding Mg(2+)
- H328 (= H316) active site, Proton donor/acceptor; mutation H->N,A: Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
- E348 (= E341) binding substrate
Sites not aligning to the query:
2pp1A Crystal structure of l-talarate/galactarate dehydratase from salmonella typhimurium lt2 liganded with mg and l-lyxarohydroxamate (see paper)
32% identity, 91% coverage: 28:380/389 of query aligns to 54:380/395 of 2pp1A
- active site: S78 (≠ A54), K192 (= K181), K194 (≠ R183), D223 (= D214), N225 (≠ H216), E249 (= E240), G274 (= G265), E275 (= E266), D298 (= D289), H325 (= H316), E345 (= E341)
- binding (2r,3s,4r)-2,3,4-trihydroxy-5-(hydroxyamino)-5-oxopentanoic acid: K79 (≠ G55), F168 (≠ E159), K194 (≠ R183), E275 (= E266), H325 (= H316), E345 (= E341)
- binding magnesium ion: D223 (= D214), E249 (= E240), E275 (= E266)
Sites not aligning to the query:
Query Sequence
>YP_001314493.1 NCBI__GCF_000017145.1:YP_001314493.1
MRIKTVQAWWVRIPIEASRQHRSDFGRLTTFDAAILRIETNDGIVGWGEGKNAAGSAGTY
GTLVHMLNHEVGRQLIGRDPADISTIWEMLYNGTRHETAAASGHAMPELARRGLSVAAIS
AVDIALWDILGKSLGVPVWKLLGGRKADRLPAYASGGWETAEKIGDQLRSYIASGGFKSV
KMRVGAMDAGPHVSAARVRAARKALGPSVDLMVDAHGTYTVADAKRFIQLVQDFDLAWFE
EPVNADDKYGMAEVRAAGNVPIAAGESEATRFAFRDLAVLRAVDIFQPDPAFCGGITEAM
RIGAIASAFNLRFAPHLWAGAPCFFSGLQICAASPASFVVEYSLGANPMVHDLVEDTVAV
KDGMIEIPDRPGLGFTINERVLENHAQRL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory