SitesBLAST
Comparing YP_004140783.1 NCBI__GCF_000185905.1:YP_004140783.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 11 hits to proteins with known functional sites (download)
1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
66% identity, 100% coverage: 2:309/309 of query aligns to 1:307/307 of 1efpA
- binding flavin-adenine dinucleotide: G199 (= G200), R200 (= R201), G201 (≠ A202), S225 (= S227), R226 (= R228), A227 (= A229), Q239 (= Q241), V240 (= V242), G241 (= G243), T243 (= T245), G256 (= G258), I257 (= I259), S258 (= S260), A260 (= A262), Q262 (= Q264), H263 (= H265), N277 (= N279), K278 (= K280), D295 (= D297), L296 (= L298)
P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
54% identity, 99% coverage: 4:309/309 of query aligns to 23:330/333 of P13804
- G116 (= G95) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
- T171 (≠ A150) to I: decreased protein stability; dbSNP:rs1801591
- R223 (= R201) binding FAD
- S248 (= S227) binding FAD
- R249 (= R228) mutation to A: Loss of electron transfer activity.
- VGQT 263:266 (= VGQT 242:245) binding FAD
- T266 (= T245) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
- SGAIQH 281:286 (= SGAIQH 260:265) binding FAD
- N300 (= N279) binding FAD
- DL 318:319 (= DL 297:298) binding FAD
Sites not aligning to the query:
- 20:204 Domain I
- 205:333 Domain II
2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
54% identity, 99% coverage: 4:309/309 of query aligns to 5:312/315 of 2a1uA
- binding flavin-adenine dinucleotide: G204 (= G200), R205 (= R201), S230 (= S227), R231 (= R228), A232 (= A229), Q244 (= Q241), V245 (= V242), G246 (= G243), T248 (= T245), G261 (= G258), I262 (= I259), S263 (= S260), A265 (= A262), Q267 (= Q264), H268 (= H265), N282 (= N279), K283 (= K280), D300 (= D297), L301 (= L298)
6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
37% identity, 92% coverage: 25:309/309 of query aligns to 94:388/393 of 6fahE
- binding flavin-adenine dinucleotide: L180 (≠ Q110), R200 (= R125), M281 (≠ R201), G282 (≠ A202), R307 (= R228), A308 (= A229), Q320 (= Q241), V321 (= V242), G322 (= G243), Q323 (= Q244), T324 (= T245), G337 (= G258), I338 (= I259), S339 (= S260), Q343 (= Q264), H344 (= H265), N358 (= N279), K359 (= K280), L377 (= L298)
Sites not aligning to the query:
- binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49
5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
35% identity, 98% coverage: 3:305/309 of query aligns to 4:320/331 of 5ol2A
- binding calcium ion: E75 (= E71), D188 (vs. gap)
- binding flavin-adenine dinucleotide: T117 (≠ V111), R136 (= R125), I147 (≠ V136), G216 (= G200), R217 (= R201), G218 (≠ A202), S242 (= S227), R243 (= R228), A244 (= A229), Q256 (= Q241), V257 (= V242), G258 (= G243), T260 (= T245), G273 (= G258), I274 (= I259), S275 (= S260), A277 (= A262), Q279 (= Q264), H280 (= H265), N294 (= N279), K295 (= K280), D312 (= D297), V313 (≠ L298)
5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
40% identity, 73% coverage: 84:309/309 of query aligns to 69:291/292 of 5ow0A
- binding flavin-adenine dinucleotide: G183 (= G200), R184 (= R201), G185 (≠ A202), S209 (= S227), R210 (= R228), Q223 (= Q241), I224 (≠ V242), G225 (= G243), T227 (= T245), G240 (= G258), V241 (≠ I259), S242 (= S260), A244 (= A262), Q246 (= Q264), H247 (= H265), N261 (= N279), K262 (= K280), D279 (= D297), Y280 (≠ L298)
4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
35% identity, 71% coverage: 86:305/309 of query aligns to 101:328/338 of 4kpuA
- binding flavin-adenine dinucleotide: L125 (≠ Q110), R144 (= R125), I155 (≠ V136), G224 (= G200), R225 (= R201), G226 (≠ A202), S250 (= S227), R251 (= R228), A252 (= A229), Q264 (= Q241), V265 (= V242), G266 (= G243), Q267 (= Q244), S268 (≠ T245), G281 (= G258), I282 (= I259), S283 (= S260), S285 (≠ A262), Q287 (= Q264), H288 (= H265), N302 (= N279), K303 (= K280), D320 (= D297), A321 (≠ L298)
7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
33% identity, 93% coverage: 20:305/309 of query aligns to 34:327/337 of 7qh2A
- binding flavin-adenine dinucleotide: L125 (vs. gap), T126 (vs. gap), R144 (= R125), I155 (≠ T135), R224 (= R201), G225 (≠ A202), T249 (≠ S227), R250 (= R228), Q263 (= Q241), I264 (≠ V242), G265 (= G243), L266 (≠ Q244), S267 (≠ T245), G280 (= G258), I281 (= I259), S282 (= S260), Q286 (= Q264), N301 (= N279), S302 (≠ K280), D303 (= D281), D319 (= D297), L320 (= L298)
7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
35% identity, 94% coverage: 19:309/309 of query aligns to 29:327/336 of 7koeB
- binding flavin-adenine dinucleotide: T121 (≠ V111), R140 (= R125), T142 (≠ I127), G219 (= G200), K220 (≠ R201), G221 (≠ A202), S245 (= S227), R246 (= R228), A247 (= A229), Q259 (= Q241), V260 (= V242), G261 (= G243), Q262 (= Q244), T263 (= T245), G276 (= G258), S278 (= S260), Q282 (= Q264), H283 (= H265), N297 (= N279), I298 (≠ K280), L316 (= L298)
P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
40% identity, 54% coverage: 144:309/309 of query aligns to 153:319/321 of P53571
- R211 (= R201) binding FAD
- SR 236:237 (= SR 227:228) binding FAD
- QVGQS 250:254 (≠ QVGQT 241:245) binding FAD
- 268:275 (vs. 258:265, 88% identical) binding FAD
- N289 (= N279) binding FAD
- DI 307:308 (≠ DL 297:298) binding FAD
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
39% identity, 54% coverage: 144:309/309 of query aligns to 152:318/319 of 3clrD
- binding flavin-adenine dinucleotide: G209 (= G200), R210 (= R201), G211 (≠ A202), S235 (= S227), A236 (≠ R228), P237 (≠ A229), Q249 (= Q241), V250 (= V242), G251 (= G243), Q252 (= Q244), S253 (≠ T245), G267 (= G258), I268 (= I259), S269 (= S260), S271 (≠ A262), Q273 (= Q264), H274 (= H265), N288 (= N279), T289 (≠ K280), D306 (= D297), I307 (≠ L298)
Query Sequence
>YP_004140783.1 NCBI__GCF_000185905.1:YP_004140783.1
MAILLIAEHDNATLSDQTAKALSAALQIGSDVHVLVAGKGAKPAADAAAKLKGVSKVLLA
EADELAERLAEPTAALIVSLAGAYDTIIAPATSSGKNVAPRVAALLDVAQVSEIIEVVSP
DTFKRPIYAGNAIQTVQSSDAKKVITVRTASFQAAPEGGSAAVETVKVAANPGLSTFIEN
KLSETDRPELTSAKIIISGGRALGSSEKFQEVILPVADKLGAAVGASRAAVDAGYAPNDW
QVGQTGKVVAPDLYIAVGISGAIQHLAGMKDSKVIVAINKDEEAPIFQVADYGLVGDLFV
ILPELQKAL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory