SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H31) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D35) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ Y82) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ H107) mutation to H: Little effect on the kinetic properties.
E349 (= E374) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
32% identity, 55% coverage: 19:389/673 of query aligns to 17:347/497 of 1ct9A

query
sites
1ct9A

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A

active site: L50 (= L52), N74 (= N76), G75 (= G77), T305 (≠ R349), R308 (vs. gap), E332 (= E374)
binding adenosine monophosphate: L232 (≠ Y271), L233 (= L272), S234 (= S273), S239 (= S278), A255 (≠ S297), V256 (≠ L298), D263 (≠ E307), M316 (≠ L358), S330 (≠ T372), G331 (= G373), E332 (= E374)
binding glutamine: R49 (= R51), L50 (= L52), I52 (≠ V54), V53 (≠ I55), N74 (= N76), G75 (= G77), E76 (= E78), D98 (= D101)

Sites not aligning to the query:

active site: 1, 366
binding glutamine: 1

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 57% coverage: 1:386/673 of query aligns to 1:368/557 of P78753

query
sites
P78753

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Sites not aligning to the query:

391 modified: Phosphoserine
489 modified: Phosphoserine

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
27% identity, 58% coverage: 1:389/673 of query aligns to 1:380/561 of P08243

query
sites
P08243

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A

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (vs. gap) to E: in dbSNP:rs1049674
F362 (vs. gap) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
29% identity, 57% coverage: 5:388/673 of query aligns to 1:366/509 of 6gq3A

query
sites
6gq3A

A
x
C

G

G

I

I

V
x
W

A

A

L

L

N

F

A

G

A

S

A

D

E

D

P

C

P

L

S

S

R

V

A

Q

A

C

L

L

L

S

R

A

M

M

A

K

A

I

A

A

L

-

S

-

H

H

R

R

G

G

P

P

D

D

E

A

-

F

-

R

-

F

S

E

G

N

V

V

Y

N

R

G

D

Y

R

T

R

N

A

C

G

C

L

F

A

G

H

F

T

H

R
|
R

L
|
L

S

A

V
|
V

I
x
V

D

D

L

P

S

L

T

F

G

G

Q

M

Q

Q

P

P

L

I

-

R

A

V

D

K

T

K

G

Y

D

P

T

Y

T

L

W

W

I

L

V

C

F
x
Y

N
|
N

G
|
G

E
|
E

I

I

F

Y

N

N

Y

H

V

K

E

K

L

M

R

Q

E

Q

Q

H

L

F

M

-

A

-

L

-

G

E

H

F

R

E

F

Y

R

Q

T

T

R

K

S
x
V

D
|
D

T

G

E

E

V

I

I

I

V

L

H

H

A

L

Y

Y

R

D

A

K

W

G

G

G

V

I

-

E

A

Q

A

T

F

I

E

C

R

M

L

L

N

D

G

G

Q

V

W

F

A

A

L

F

A

V

I

L

W

L

D

D

S

T

L

A

T

N

G

K

R

K

L

V

V

F

L

L

S

G

R

R

D

D

R

T

F

Y

G

G

I

V

C

R

P

P

L

L

-

F

H

K

L

A

C

M

E

T

H

E

A

D

G

G

R

F

L

L

H

A

F

V

A

C

S

S

E

E

V

A

K

K

A

G

I

L

F

V

A

-

D

-

P

-

A

-

I

-

P

-

R

-

A

-

F

-

D

-

P

-

A

-

G

-

I

-

D

-

Q

-

T

-

F

-

T

T

L

L

W

K

T

H

V

S

V

A

P

T

P

P

Q

-

G

-

V

-

F

F

Q

L

G

K

V

V

K

E

E

P

L

F

T

L

P

P

G

G

H

H

V

Y

R

E

I

V

Y

L

D

D

-

L

-

K

-

P

N

N

G

G

S

K

V

V

R

A

E

S

H

V

A

E

F

M

W

V

K

K

P

-

C

-

Y

Y

P

H

E

H

I

C

A

R

D

D

Q

-

A

-

H

-

G

-

T

V

F

F

T

P

G

G

-

F

S

E

L

I

D

E

D

T

A

V

V

K

D

N

E

N

V

L

R

R

S

I

A

L

L

F

Q

N

A

N

A

A

T

V

A

K

L

K

R

R

M

L

V

M

K

-

A

T

D

D

V

R

P

R

V

I

G

G

C

C

Y

L

L

L

S

S

G

G

L

L

D

D

S

S

L

L

V

V

-

A

A

A

M

T

L

L

G

L

R

K

R

Q

F

L

A

K

G

E

A

A

-

Q

-

V

-

Q

-

Y

P

P

F

L

Q

Q

T

T

F

F

S

A

L

I

R

G

F

M

A

E

D

D

A

S

E

P

Y

-

D

-

E

D

T

L

R

L

Y

A

Q

A

R

R

L

K

V

V

A

A

S

D

A

H

S

I

G

G

G

S

E

E

H

H

H

Y

E

E

V

V

M

L

V

F

S

N

R

S

G

E

D

E

I

G

A

I

E

Q

V

A

F

L

P

D

D

E

V

V

I

I

R

F

H

S

A

L

E

E

R

T

P

Y

I

D

L

I

R
x
T

T

T

-

V

-
x
R

A

A

P

S

A

V

P

G

L

M

F

Y

L

L

L

I

S

S

R

K

L

Y

V

I

R

R

E

K

H

N

G

T

I

D

K

S

V

V

L

I

-

F

T

S

G

G

E

E

G

G

A

S

D

D

E

E

M

L

F

T

A

Q

G

G

Y

Y

D

I

L

Y

F

F

R

H

E

K

active site: W4 (≠ V8), L49 (= L52), N74 (= N76), G75 (= G77), T324 (≠ R349), R327 (vs. gap)
binding 5-oxo-l-norleucine: C1 (≠ A5), R48 (= R51), V51 (= V54), V52 (≠ I55), Y73 (≠ F75), N74 (= N76), G75 (= G77), E76 (= E78), V95 (≠ S100), D96 (= D101)

1jgtB Crystal structure of beta-lactam synthetase (see paper)
28% identity, 48% coverage: 67:391/673 of query aligns to 64:360/500 of 1jgtB

query
sites
1jgtB

T

T

G

G

D

A

T

P

T

T

W

T

I

A

V

V

F

L

N
x
A

G
|
G

E

E

I

I

F

Y

N

N

Y

R

V

D

E

E

L

L

R

-

E

-

Q

-

L

L

M

S

A

V

L

L

G

P

H

A

R

G

F

P

R

A

T

P

R

E

S

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

Y

L

R

E

A

R

W

Y

G

D

V

L

A

H

A

A

F

F

E

R

R

L

L

V

N

N

G

G

Q

R

W

F

A

A

L

T

A

V

I

V

W

R

D

-

S

-

L

-

T

T

G

G

-

D

R

R

L

V

V

L

L

L

S

A

R

T

D

D

R

H

F

A

G

G

I

S

C

V

P

P

L

L

H

Y

L

T

C

C

E

V

H

A

A

P

G

G

R

E

L

V

H

R

F

A

A

S

S

T

E

E

V

A

K

K

A

A

I

L

F

A

A

A

A

H

D

R

P

-

A

-

I

-

P

-

R

-

A

-

F

-

D

D

P

P

A

K

G

G

I

-

D

-

Q

-

T

-

F

F

T

P

L

L

W

A

T

D

V

A

V

R

P

R

P

V

Q

A

G

G

V

-

F

L

Q

T

G

G

V

V

K

Y

E

Q

L

V

T

P

P

A

G

G

H

A

V

V

R

M

I

D

Y

I

D

D

-

L

-

G

N

S

G

G

S

T

V

A

R

V

E

T

H

H

A

R

F

T

W

W

K

T

P

P

-

G

-

L

-

S

-

R

C

I

Y

L

P

P

E

E

I

-

A

-

D

-

Q

-

A

-

H

-

G

-

T

-

F

-

T

-

G

-

S

-

L

-

D

G

D

E

A

A

V

V

D

A

E

A

V

V

R

R

S

A

A

A

L

L

Q

E

A

K

A

A

T

V

A

A

L

Q

R

R

M

V

V

T

K

P

A

G

D

D

V

T

P

P

V

L

G

-

C

V

Y
x
V

L
|
L

S
|
S

G

G

G
|
G

L
x
I

D
|
D

S
|
S

S

S

L

G

V

V

A

A

M

A

L

C

G

A

R

H

R

R

F

A

A

A

G

G

A

-

P

-

F

-

Q

E

T

L

F

D

S

T

L

V

R
x
S

F
x
M

A

G

D

T

A

D

E

T

Y

S

D

N

E

E

T

F

R

R

Y

E

Q

A

R

R

L

A

V

V

A

V

S

D

A

H

S

L

G

R

G

T

E

R

H

H

H

R

E

E

V

I

M

T

V

I

S

P

R

T

G

T

D

E

I

L

A

L

E

A

V

Q

F

L

P

P

D

Y

V

A

I

V

R

W

H

A

A

S

E

E

R

S

-

V

-

D

P

P

I
x
D

L

I

R

I

T

E

A
x
Y

P

L

A

L

P

P

L
|
L

F

T

L

A

L

L

S

Y

R

R

L

A

V

L

R

-

E

D

H

G

G

P

I

E

K

R

V

R

V

I

L

L

T

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

M

P

F

L

A

G

G

G

Y

-

D

-

L
x
M

F

H

R

R

E

E

G

D

K

R

V

L

active site: A73 (≠ N76), G74 (= G77), D319 (≠ I347), Y345 (≠ E374)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ Y271), L245 (= L272), S246 (= S273), G248 (= G275), I249 (≠ L276), D250 (= D277), S251 (= S278), S269 (≠ R299), M270 (≠ F300), L327 (= L355), G344 (= G373), Y345 (≠ E374), D348 (= D377)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ A351), Y345 (≠ E374), G346 (= G375), D348 (= D377), I349 (≠ E378), M354 (≠ L385)
binding magnesium ion: D250 (= D277), D348 (= D377)

Sites not aligning to the query:

active site: 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 420, 440
binding n2-(carboxyethyl)-l-arginine: 370, 379

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
28% identity, 48% coverage: 67:391/673 of query aligns to 56:347/491 of 1mc1A

query
sites
1mc1A

T

T

G

G

D

A

T

P

T

T

W

T

I

A

V

V

F

L

N
x
A

G
|
G

E

E

I

I

F

Y

N

N

Y

R

V

D

E

E

L

L

R

-

E

-

Q

-

L

L

M

S

A

V

L

L

G

P

H

A

R

G

F

P

R

A

T

P

R

E

S

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

Y

L

R

E

A

R

W

Y

G

D

V

L

A

H

A

A

F

F

E

R

R

L

L

V

N

N

G

G

Q

R

W

F

A

A

L

T

A

V

I

V

W

R

D

-

S

-

L

-

T

T

G

G

-

D

R

R

L

V

V

L

L

L

S

A

R

T

D

D

R

H

F

A

G

G

I

S

C

V

P

P

L

L

H

Y

L

T

C

C

E

V

H

A

A

P

G

G

R

E

L

V

H

R

F

A

A

S

S

T

E

E

V

A

K

K

A

A

I

L

F

A

A

A

A

G

D

F

P

P

A

-

I

L

P

A

R

D

A

A

F

R

D

R

P

V

A

A

G

G

I

L

D

-

Q

-

T

-

F

-

T

-

L

-

W

-

T

-

V

-

P

-

Q

-

G

-

V

-

F

-

Q

T

G

G

V

V

K

Y

E

Q

L

V

T

P

P

A

G

G

H

A

V

V

R

M

I

D

Y

I

D

D

-

L

-

G

N

S

G

G

S

T

V

A

R

V

E

T

H

H

A

R

F

T

W

W

K

T

P

P

-

G

-

L

-

S

-

R

C

I

Y

L

P

P

E

E

I

-

A

-

D

-

Q

-

A

-

H

-

G

-

T

-

F

-

T

-

G

-

S

-

L

-

D

G

D

E

A

A

V

V

D

A

E

A

V

V

R

R

S

A

A

A

L

L

Q

E

A

K

A

A

T

V

A

A

L

Q

R

R

M

V

V

T

K

P

A

G

D

D

V

T

P

P

V

L

G

-

C

V

Y
x
V

L

L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

V

V

A

A

M

A

L

C

G

A

R

H

R

R

F

A

A

A

G

G

A

-

P

-

F

-

Q

E

T

L

F

D

S

T

L

V

R
x
S

F
x
M

A

G

D

T

A

D

E

T

Y

S

D

N

E

E

T

F

R

R

Y

E

Q

A

R

R

L

A

V

V

A

V

S

D

A

H

S

L

G

R

G

T

E

R

H

H

H

R

E

E

V

I

M

T

V

I

S

P

R

T

G

T

D

E

I

L

A

L

E

A

V

Q

F

L

P

P

D

Y

V

A

I

V

R

W

H

A

A

S

E

E

R

S

-

V

-

D

P

P

I
x
D

L

I

R

I

T

E

A
x
Y

P

L

A

L

P

P

L

L

F

T

L

A

L

L

S

Y

R

R

L

A

V

L

R

-

E

D

H

G

G

P

I

E

K

R

V

R

V

I

L

L

T

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

M

P

F

L

A

G

G

G

Y

-

D

-

L

M

F

H

R

R

E

E

G

D

K

R

V

L

active site: A65 (≠ N76), G66 (= G77), D306 (≠ I347), Y332 (≠ E374)
binding adenosine monophosphate: V231 (≠ Y271), S233 (= S273), S238 (= S278), S256 (≠ R299), M257 (≠ F300), G331 (= G373)
binding magnesium ion: D237 (= D277), D335 (= D377)
binding deoxyguanidinoproclavaminic acid: Y310 (≠ A351), Y332 (≠ E374), G333 (= G375), I336 (≠ E378)
binding pyrophosphate 2-: S233 (= S273), G235 (= G275), D237 (= D277), S238 (= S278), D335 (= D377)

Sites not aligning to the query:

active site: 366, 427
binding adenosine monophosphate: 427, 430
binding deoxyguanidinoproclavaminic acid: 357, 366, 427
binding pyrophosphate 2-: 407, 427, 428

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
27% identity, 48% coverage: 67:391/673 of query aligns to 60:352/496 of 1mbzA

query
sites
1mbzA

T

T

G

G

D

A

T

P

T

T

W

T

I

A

V

V

F

L

N
x
A

G
|
G

E

E

I

I

F

Y

N

N

Y

R

V

D

E

E

L

L

R

-

E

-

Q

-

L

L

M

S

A

V

L

L

G

P

H

A

R

G

F

P

R

A

T

P

R

E

S

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

Y

L

R

E

A

R

W

Y

G

D

V

L

A

H

A

A

F

F

E

R

R

L

L

V

N

N

G

G

Q

R

W

F

A

A

L

T

A

V

I

V

W

R

D

-

S

-

L

-

T

T

G

G

-

D

R

R

L

V

V

L

L

L

S

A

R

T

D

D

R

H

F

A

G

G

I

S

C

V

P

P

L

L

H

Y

L

T

C

C

E

V

H

A

A

P

G

G

R

E

L

V

H

R

F

A

A

S

S

T

E

E

V

A

K

K

A

A

I

L

F

A

A

A

A

H

D

G

P

F

A

P

I

L

P

A

R

D

A

A

F

R

D

R

P

V

A

A

G

G

I

L

D

-

Q

-

T

-

F

-

T

-

L

-

W

-

T

-

V

-

P

-

Q

-

G

-

V

-

F

-

Q

T

G

G

V

V

K

Y

E

Q

L

V

T

P

P

A

G

G

H

A

V

V

R

M

I

D

Y

I

D

D

-

L

-

G

N

S

G

G

S

T

V

A

R

V

E

T

H

H

A

R

F

T

W

W

K

T

P

P

-

G

-

L

-

S

-

R

C

I

Y

L

P

P

E

E

I

-

A

-

D

-

Q

-

A

-

H

-

G

-

T

-

F

-

T

-

G

-

S

-

L

-

D

G

D

E

A

A

V

V

D

A

E

A

V

V

R

R

S

A

A

A

L

L

Q

E

A

K

A

A

T

V

A

A

L

Q

R

R

M

V

V

T

K

P

A

G

D

D

V

T

P

P

V

L

G

-

C

V

Y
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

V

V

A

A

M

A

L

C

G

A

R

H

R

R

F

A

A

A

G

G

A

-

P

-

F

-

Q

E

T

L

F

D

S

T

L

V

R
x
S

F
x
M

A

G

D

T

A

D

E

T

Y

S

D

N

E

E

T

F

R

R

Y

E

Q

A

R

R

L

A

V

V

A

V

S

D

A

H

S

L

G

R

G

T

E

R

H

H

H

R

E

E

V

I

M

T

V

I

S

P

R

T

G

T

D

E

I

L

A

L

E

A

V

Q

F

L

P

P

D

Y

V

A

I

V

R

W

H

A

A

S

E

E

R

S

-

V

-

D

P

P

I
x
D

L

I

R

I

T

E

A
x
Y

P

L

A

L

P

P

L
|
L

F

T

L

A

L

L

S

Y

R

R

L

A

V

L

R

-

E

D

H

G

G

P

I

E

K

R

V

R

V

I

L

L

T

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

M

P

F

L

A

G

G

G

Y

-

D

-

L

M

F

H

R

R

E

E

G

D

K

R

V

L

active site: A69 (≠ N76), G70 (= G77), D311 (≠ I347), Y337 (≠ E374)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ Y271), L237 (= L272), S238 (= S273), S243 (= S278), S261 (≠ R299), M262 (≠ F300), Y315 (≠ A351), L319 (= L355), G336 (= G373), Y337 (≠ E374), G338 (= G375), D340 (= D377), I341 (≠ E378)
binding magnesium ion: D242 (= D277), D340 (= D377)
binding pyrophosphate 2-: S238 (= S273), G240 (= G275), D242 (= D277), S243 (= S278), D340 (= D377)

Sites not aligning to the query:

active site: 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 362, 371, 432, 434, 435
binding pyrophosphate 2-: 412, 432, 433

1mb9A Beta-lactam synthetase complexed with atp (see paper)
28% identity, 48% coverage: 67:391/673 of query aligns to 61:351/485 of 1mb9A

query
sites
1mb9A

T

T

G

G

D

A

T

P

T

T

W

T

I

A

V

V

F

L

N
x
A

G
|
G

E

E

I

I

F

Y

N

N

Y

R

V

D

E

E

L

L

R

-

E

-

Q

-

L

L

M

S

A

V

L

L

G

P

H

A

R

G

F

P

R

A

T

P

R

E

S

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

Y

L

R

E

A

R

W

Y

G

D

V

L

A

H

A

A

F

F

E

R

R

L

L

V

N

N

G

G

Q

R

W

F

A

A

L

T

A

V

I

V

W

R

D

-

S

-

L

-

T

T

G

G

-

D

R

R

L

V

V

L

L

L

S

A

R

T

D

D

R

H

F

A

G

G

I

S

C

V

P

P

L

L

H

Y

L

T

C

C

E

V

H

A

A

P

G

G

R

E

L

V

H

R

F

A

A

S

S

T

E

E

V

A

K

K

A

A

I

L

F

A

A

A

A

F

D

P

P

L

A

A

I

D

P

A

R

R

A

R

F

V

D

-

P

-

A

A

G

G

I

L

D

-

Q

-

T

-

F

-

T

-

L

-

W

-

T

-

V

-

P

-

Q

-

G

-

V

-

F

-

Q

T

G

G

V

V

K

Y

E

Q

L

V

T

P

P

A

G

G

H

A

V

V

R

M

I

D

Y

I

D

D

-

L

-

G

N

S

G

G

S

T

V

A

R

V

E

T

H

H

A

R

F

T

W

W

K

T

P

P

-

G

-

L

-

S

-

R

C

I

Y

L

P

P

E

E

I

-

A

-

D

-

Q

-

A

-

H

-

G

-

T

-

F

-

T

-

G

-

S

-

L

-

D

G

D

E

A

A

V

V

D

A

E

A

V

V

R

R

S

A

A

A

L

L

Q

E

A

K

A

A

T

V

A

A

L

Q

R

R

M

V

V

T

K

P

A

G

D

D

V

T

P

P

V

L

G

-

C

V

Y
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

V

V

A

A

M

A

L

C

G

A

R

H

R

R

F

A

A

A

G

G

A

-

P

-

F

-

Q

E

T

L

F

D

S

T

L

V

R
x
S

F
x
M

A

G

D

T

A

D

E

T

Y

S

D

N

E

E

T

F

R

R

Y

E

Q

A

R

R

L

A

V

V

A

V

S

D

A

H

S

L

G

R

G

T

E

R

H

H

H

R

E

E

V

I

M

T

V

I

S

P

R

T

G

T

D

E

I

L

A

L

E

A

V

Q

F

L

P

P

D

Y

V

A

I

V

R

W

H

A

A

S

E

E

R

S

-

V

-

D

P

P

I
x
D

L

I

R

I

T

E

A
x
Y

P

L

A

L

P

P

L
|
L

F

T

L

A

L

L

S

Y

R

R

L

A

V

L

R

-

E

D

H

G

G

P

I

E

K

R

V

R

V

I

L

L

T

T

G
|
G

E
x
Y

G

G

A

A

D
|
D

E

I

M

P

F

L

A

G

G

G

Y

-

D

-

L

M

F

H

R

R

E

E

G

D

K

R

V

L

active site: A70 (≠ N76), G71 (= G77), D310 (≠ I347), Y336 (≠ E374)
binding adenosine monophosphate: V235 (≠ Y271), L236 (= L272), S242 (= S278), S260 (≠ R299), M261 (≠ F300), Y314 (≠ A351), L318 (= L355), G335 (= G373), Y336 (≠ E374)
binding adenosine-5'-triphosphate: V235 (≠ Y271), L236 (= L272), S237 (= S273), G239 (= G275), D241 (= D277), S242 (= S278), S260 (≠ R299), M261 (≠ F300), L318 (= L355), G335 (= G373), D339 (= D377)
binding magnesium ion: D241 (= D277), D339 (= D377)
binding pyrophosphate 2-: S237 (= S273), G239 (= G275), D241 (= D277), S242 (= S278), D339 (= D377)

Sites not aligning to the query:

active site: 370, 431
binding adenosine-5'-triphosphate: 411, 431
binding pyrophosphate 2-: 411, 431

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
26% identity, 34% coverage: 70:299/673 of query aligns to 182:392/561 of Q9STG9

query
sites
Q9STG9

T

S

T

V

W

G

I

V

V

A

F
x
H

N

N

G

G

E

N

I

L

F

V

N

N

Y

Y

V

T

E

K

L

L

R

R

E

A

Q

D

L

L

M

E

A

E

L

N

G

G

H

S

R

I

F

F

R

N

T

T

R

S

S

S

D

D

T

T

E

E

V

V

I

V

V

L

H

H

-

L

-

I

-

A

-

I

-

S

-

K

-

A

-

R

A

P

Y

F

R

F

A

M

W

R

G

I

V

V

A

D

A

A

F

C

E

E

R

K

L

L

N

Q

G

G

Q

A

W

Y

A

S

L

M

A

V

I

F

W

V

D

T

S

E

L

-

T

-

G

D

R

K

L

L

V

V

L

A

S

V

R

R

D

D

R

P

F

H

G

G

I

F

C
x
R

P
|
P

L

L

H

V

L

M

C

G

E

R

H

R

A

S

-

N

G

G

R

A

L

V

H

V

F

F

A

A

S

S

E

E

V

T

K

C

A

A

I

L

F

D

A

L

A

-

D

-

P

-

A

-

I

-

P

-

R

-

A

-

F

-

D

-

P

-

A

-

G

-

I

I

D

E

Q

A

T

T

F

Y

T

E

L

-

W

-

T

-

V

-

P

-

Q

-

G

-

V

-

F

-

Q

-

G

-

V

-

K

R

E

E

L

V

T

Y

P

P

G

G

H

E

V

V

R

L

I

V

Y

V

D

D

N

K

G

D

S

G

V

V

R

K

E

C

H

Q

A

C

F

L

W

M

K

P

P

H

C

P

Y

E

P

P

E

K

I

Q

A

C

D

I

Q

F

A

E

H

H

G

I

T

Y

F

F

T

-

G

-

S

S

L

L

D

P

D

N

A

S

V

I

D

V

E

F

V

G

R

R

S

S

A

V

L

Y

Q

E

A

S

A

R

T

H

A

V

L

F

-

G

R

E

M

I

V

L

K

A

A

T

D

E

V

S

P

P

V

V

G

D

C

C

Y

D

L

V

S

V

G

I

G

A

L

V

-

P

D

D

S

S

S
x
G

L

V

V

V

A

A

M

A

L

L

G

G

R

Y

R

A

F

A

-

K

A

A

G

G

A

V

P

A

F

F

Q

Q

T

Q

F

G

S

L

L

I

R

R

H187 (≠ F75) mutation to T: In cia1-2; small plants with white leaves showing an irregular mosaic of green sectors.
R264 (≠ C146) mutation to K: Strong resistance to the bleaching herbicides DAS073 and DAS734.
P265 (= P147) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494.
G371 (≠ S279) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734.

Sites not aligning to the query:

476 P→S: Resistance to the bleaching herbicides DAS073 and DAS734.
494 Y→F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265.

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
26% identity, 34% coverage: 70:299/673 of query aligns to 96:306/460 of 6lbpA

query
sites
6lbpA

T

S

T

V

W

G

I

V

V

A

F

H

N
|
N

G
|
G

E

N

I

L

F

V

N

N

Y

Y

V

T

E

K

L

L

R

R

E

A

Q

D

L

L

M

E

A

E

L

N

G

G

H

S

R

I

F

F

R

N

T

T

R

S

S

S

D

D

T

T

E

E

V

V

I

V

V

L

H

H

-

L

-

I

-

A

-

I

-

S

-

K

-

A

-

R

A

P

Y

F

R

F

A

M

W

R

G

I

V

V

A

D

A

A

F

C

E

E

R

K

L

L

N

Q

G

G

Q

A

W

Y

A

S

L

M

A

V

I

F

W

V

D

T

S

E

L

-

T

-

G

D

R

K

L

L

V

V

L

A

S

V

R

R

D

D

R

P

F

H

G

G

I

F

C

R

P

P

L

L

H

V

L

M

C

G

E

R

H

R

A

S

-

N

G

G

R

A

L

V

H

V

F

F

A

A

S

S

E

E

V

T

K

C

A

A

I

L

F

D

A

L

A

-

D

-

P

-

A

-

I

-

P

-

R

-

A

-

F

-

D

-

P

-

A

-

G

-

I

I

D

E

Q

A

T

T

F

Y

T

E

L

-

W

-

T

-

V

-

P

-

Q

-

G

-

V

-

F

-

Q

-

G

-

V

-

K

R

E

E

L

V

T

Y

P

P

G

G

H

E

V

V

R

L

I

V

Y

V

D

D

N

K

G

D

S

G

V

V

R

K

E

C

H

Q

A

C

F

L

W

M

K

P

P

H

C

P

Y

E

P

P

E

K

I

Q

A
x
C

D

I

Q
x
F

A

E

H

H

G

I

T
x
Y

F

F

T

-

G

-

S

S

L

L

D

P

D

N

A

S

V

I

D

V

E

F

V

G

R

R

S

S

A

V

L

Y

Q

E

A

S

A

R

T

H

A

V

L

F

-

G

R

E

M

I

V

L

K

A

A

T

D

E

V

S

P

P

V

V

G

D

C

C

Y

D

L

V

S

V

G

I

G

A

L

V

-

P

D

D

S

S

S

G

L

V

V

V

A

A

M

A

L

L

G

G

R

Y

R

A

F

A

-

K

A

A

G

G

A

V

P

A

F

F

Q
|
Q

T

Q

F

G

S

L

L

I

R
|
R

active site: N102 (= N76), G103 (= G77), Y243 (≠ T237), Q301 (= Q294), R306 (= R299)
binding iron/sulfur cluster: C237 (≠ A231), F239 (≠ Q233)

Sites not aligning to the query:

active site: 1, 27, 316, 424
binding iron/sulfur cluster: 383, 385, 434, 436, 437

Query Sequence

>YP_004144523.1 NCBI__GCF_000185905.1:YP_004144523.1
MCGIAGIVALNAAAEPPSRAALLRMAAALSHRGPDESGVYRDRRAGLAHTRLSVIDLSTG
QQPLADTGDTTWIVFNGEIFNYVELREQLMALGHRFRTRSDTEVIVHAYRAWGVAAFERL
NGQWALAIWDSLTGRLVLSRDRFGICPLHLCEHAGRLHFASEVKAIFAADPAIPRAFDPA
GIDQTFTLWTVVPPQGVFQGVKELTPGHVRIYDNGSVREHAFWKPCYPEIADQAHGTFTG
SLDDAVDEVRSALQAATALRMVKADVPVGCYLSGGLDSSLVAMLGRRFAGAPFQTFSLRF
ADAEYDETRYQRLVASASGGEHHEVMVSRGDIAEVFPDVIRHAERPILRTAPAPLFLLSR
LVREHGIKVVLTGEGADEMFAGYDLFREGKVRRFWGRQPASARRARLLERLYPYLSRSPV
KQQAMARQFFGLGIHAFGAPGFAHDTRWRTTSAIKRLFSAGMHAEAARHDVVADLLGRLP
EEFPRWGPLAQDQYLEIRTLMSGYLLSSQGDRMLMAHSVEGRFPFLDDRLVALANSLPPD
YKLRILDEKHVLKRVAKSIVPAEVVARKKQPYRAPNALCFTGDGAPAYVREALSETALRA
ANVFDPNSVARLLDKCAAKTGDGDLSNSDNMALVGVLSTQLLHQQFVASRPSSAHAVDLR
IDVDHLHREEVLV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory