SitesBLAST
Comparing YP_004144924.1 NCBI__GCF_000185905.1:YP_004144924.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
30% identity, 90% coverage: 33:438/452 of query aligns to 5:421/425 of O59010
- S65 (≠ T87) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ S298) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ SSS 298:300) binding L-aspartate
- M311 (≠ L333) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T336) binding L-aspartate
- V355 (≠ I377) binding L-aspartate
- D394 (≠ S411) binding L-aspartate
- M395 (≠ E412) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R414) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N418) binding L-aspartate
- D405 (≠ N422) mutation to N: Strongly decreased affinity for aspartate.
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
30% identity, 89% coverage: 33:436/452 of query aligns to 5:419/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y7 (≠ A35), F46 (≠ L68), F46 (≠ L68), P75 (≠ D97), L91 (≠ T114), F95 (≠ L118), L130 (vs. gap), I133 (≠ F163), I159 (= I189), Y167 (vs. gap), K196 (= K218), G200 (≠ I222), I207 (≠ V229), F210 (= F232), L250 (≠ V272), I262 (≠ V284), M269 (≠ L291), T334 (≠ S356), V335 (= V357), G336 (≠ A358), T340 (≠ L362), L343 (≠ V365), M399 (≠ L416)
- binding aspartic acid: S277 (= S299), S278 (= S300), T314 (= T336), G354 (= G376), A358 (= A380), G359 (= G381), D394 (≠ S411), R397 (= R414), T398 (≠ A415)
- binding sodium ion: Y89 (≠ F112), T92 (≠ F115), S93 (= S116), G306 (= G328), T308 (≠ S330), N310 (= N332), N310 (= N332), M311 (≠ L333), D312 (= D334), S349 (= S371), I350 (≠ K372), T352 (≠ A374), N401 (= N418), V402 (= V419), D405 (≠ N422)
Sites not aligning to the query:
2nwwA Crystal structure of gltph in complex with tboa (see paper)
30% identity, 87% coverage: 43:433/452 of query aligns to 6:407/407 of 2nwwA
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
29% identity, 89% coverage: 33:433/452 of query aligns to 2:413/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y4 (≠ A35), G66 (= G91), V83 (≠ M109), I157 (≠ L190), Y164 (vs. gap), K193 (= K218), T305 (≠ S330), I306 (≠ F331), I347 (≠ K372)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ V44), M199 (= M224), S275 (= S300), T311 (= T336), G356 (= G381), L384 (= L404), D391 (≠ S411), R394 (= R414)
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
29% identity, 87% coverage: 43:434/452 of query aligns to 7:409/409 of 6bavA
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
29% identity, 83% coverage: 65:440/452 of query aligns to 41:423/427 of 5e9sA
- binding aspartic acid: R274 (≠ S298), S275 (= S299), S276 (= S300), T313 (= T336), G353 (= G376), V354 (≠ I377), A357 (= A380), G358 (= G381), D394 (≠ S411), R397 (= R414), T398 (≠ A415)
- binding decyl-beta-d-maltopyranoside: L194 (≠ K218), G198 (≠ I222), Y202 (≠ A226)
- binding sodium ion: Y87 (≠ F112), T90 (≠ F115), S91 (= S116), S276 (= S300), G305 (= G328), A306 (≠ Y329), T307 (≠ S330), N309 (= N332), N309 (= N332), M310 (≠ L333), D311 (= D334), S348 (= S371), I349 (≠ K372), G350 (= G373), T351 (≠ A374), N401 (= N418), V402 (= V419), D405 (≠ N422)
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
29% identity, 83% coverage: 65:440/452 of query aligns to 41:423/426 of 6xwnB
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
29% identity, 83% coverage: 65:440/452 of query aligns to 39:421/425 of 6zgbA
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
29% identity, 83% coverage: 65:440/452 of query aligns to 38:420/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (≠ K218), G195 (≠ I222), R282 (≠ E309)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S298), S272 (= S299), S273 (= S300), M307 (≠ L333), T310 (= T336), G353 (= G379), A354 (= A380), R394 (= R414), T395 (≠ A415)
Sites not aligning to the query:
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
29% identity, 87% coverage: 43:433/452 of query aligns to 7:408/408 of 6bauA
- binding cysteine: S270 (= S300), M303 (≠ L333), T306 (= T336), A345 (= A375), G346 (= G376), V347 (≠ I377), G351 (= G381), D386 (≠ S411), C389 (≠ R414), T390 (≠ A415), N393 (= N418)
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
29% identity, 83% coverage: 65:440/452 of query aligns to 34:412/416 of 6r7rA