SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing YP_425871.1 NCBI__GCF_000013085.1:YP_425871.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

4ymwC Crystal structure of an amino acid abc transporter with histidines (see paper)
38% identity, 33% coverage: 255:383/387 of query aligns to 86:214/214 of 4ymwC

query
sites
4ymwC

P

P

E

A

F

F

L

T

A

A

L

G

L

V

V

S

G

A

L

L

V

A

I

I

N
|
N

A

S

S

S

A

A

V
x
Y

I

V

C

A

E

E

I

I

V

I

R

R

S

A

G

G

I

I

E

Q

A

A

V

V

P

D

E

P

G

G

Q

Q

W

N

E

E

A

A

A

A

R

R

S

S

L

L

G

G

L

M

T

T

R

H

G

A

R

M

T

A

L

M

R

R

L

Y

V

V

V

I

L

I

P

P

Q

Q

A

A

L

I

R

K

V

N

V

I

I

L

P

P

L

A

M

L

T

G

S

N

S
x
E

Y

F

L

I

S

V

L

M

T

L

K

K

N
x
E

S

S

S

A

L

I

A

V

V

S

A

V

I

I

G

G

F

F

P

A

D

D

L

L

V

T

S

R

V

Q

V

A

N

D

T

I

S

I

A

Q

N

S

Q

V

T

T

G

Y

H

R

V

Y

F

F

E

E

A

P

I

Y

A

I

L

I

M

I

M

A

A

A

V

I

Y

Y

L

F

T

V

I

M

S

T

L

L

T

T

V

F

S

S

A

K

A

L

L

L

N

S

F

L

Y

F

N

E

H

R

R

R

L

L

binding histidine: N98 (= N267), Y102 (≠ V271), E152 (≠ S321), E159 (≠ N328)

4ymtC Crystal structure of an amino acid abc transporter complex with arginines (see paper)
38% identity, 33% coverage: 255:383/387 of query aligns to 86:214/215 of 4ymtC

query
sites
4ymtC

P

P

E

A

F

F

L

T

A

A

L

G

L

V

V

S

G

A

L

L

V

A

I

I

N
|
N

A

S

S

S

A

A

V
x
Y

I

V

C

A

E

E

I

I

V

I

R

R

S

A

G

G

I

I

E

Q

A

A

V

V

P

D

E

P

G

G

Q

Q

W

N

E

E

A

A

A

A

R

R

S

S

L

L

G

G

L

M

T

T

R

H

G

A

R

M

T

A

L

M

R

R

L

Y

V

V

V

I

L

I

P

P

Q

Q

A

A

L

I

R

K

V

N

V

I

I

L

P

P

L

A

M

L

T

G

S

N

S
x
E

Y

F

L

I

S

V

L
x
M

T

L

K
|
K

N
x
E

S

S

S

A

L

I

A

V

V

S

A

V

I

I

G

G

F

F

P

A

D

D

L

L

V

T

S

R

V

Q

V

A

N

D

T

I

S

I

A

Q

N

S

Q

V

T

T

G

Y

H

R

V

Y

F

F

E

E

A

P

I

Y

A

I

L

I

M

I

M

A

A

A

V

I

Y

Y

L

F

T

V

I

M

S

T

L

L

T

T

V

F

S

S

A

K

A

L

L

L

N

S

F

L

Y

F

N

E

H

R

R

R

L

L

binding arginine: N98 (= N267), Y102 (≠ V271), E152 (≠ S321), M156 (≠ L325), K158 (= K327), E159 (≠ N328), E159 (≠ N328)

Sites not aligning to the query:

binding arginine: 66, 67

Query Sequence

>YP_425871.1 NCBI__GCF_000013085.1:YP_425871.1
MRALGRKITWILAAWWGERPLSQLAVLLGVFALFSVLGANTVDNLGRLGITVGFGYLDHP
ANFEIGESLVAYTSGDSYARALGVGILNTALVSIIGCLLATLLGVALGIGRLSANLLISR
AVQLYVEVIRNTPLLLQLFFWSATIHALPGPRQAFEPLTGVFLTNRGLYMPALAMDGAAV
FSLLLLGGGALAGVVRILVHERRHGPMERAAKTALLGAVAVLLLGVLALCLGGAVSVEPP
RLGGFNITGGWVLSPEFLALLVGLVINASAVICEIVRSGIEAVPEGQWEAARSLGLTRGR
TLRLVVLPQALRVVIPLMTSSYLSLTKNSSLAVAIGFPDLVSVVNTSANQTGHVFEAIAL
MMAVYLTISLTVSAALNFYNHRLSLGR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory