SitesBLAST
Comparing YP_426700.1 NCBI__GCF_000013085.1:YP_426700.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P06721 Cystathionine beta-lyase MetC; CBL; CL; Beta-cystathionase MetC; Cysteine desulfhydrase MetC; CD; Cysteine lyase MetC; Cysteine-S-conjugate beta-lyase MetC; EC 4.4.1.13; EC 4.4.1.28 from Escherichia coli (strain K12) (see 2 papers)
46% identity, 98% coverage: 2:385/392 of query aligns to 5:394/395 of P06721
- K210 (= K204) modified: N6-(pyridoxal phosphate)lysine
2gqnA Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-nitro-benzamide (see paper)
46% identity, 98% coverage: 2:385/392 of query aligns to 1:390/391 of 2gqnA
- active site: R54 (= R54), Y107 (= Y107), D181 (= D179), K206 (= K204)
- binding (5-hydroxy-6-methyl-4-((2-(2-(2-nitrobenzamido)acetyl)hydrazinyl)methyl)pyridin-3-yl)methyl dihydrogen phosphate: C81 (≠ S81), G82 (= G82), A83 (≠ M83), Y107 (= Y107), E108 (≠ W108), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (= M213), Y334 (= Y332), S335 (= S333), W336 (= W334), R368 (= R363)
2fq6A Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-trifluoromethyl-benzamide (see paper)
46% identity, 98% coverage: 2:385/392 of query aligns to 1:390/391 of 2fq6A
- active site: R54 (= R54), Y107 (= Y107), D181 (= D179), K206 (= K204)
- binding phosphoric acid mono-(5-hydroxy-6-methyl-4-{[2-(2-trifluoromethyl-benzoylamino)-acetyl]-hydrazonomethyl}-pyridin-3-ylmethyl)ester: C81 (≠ S81), G82 (= G82), A83 (≠ M83), Y107 (= Y107), P109 (= P109), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (= M213), Y334 (= Y332), S335 (= S333), W336 (= W334), R368 (= R363)
1cl2A Cystathionine beta-lyase (cbl) from escherichia coli in complex with aminoethoxyvinylglycine (see paper)
46% identity, 98% coverage: 2:385/392 of query aligns to 1:390/391 of 1cl2A
- active site: R54 (= R54), Y107 (= Y107), D181 (= D179), K206 (= K204)
- binding (2E,3E)-4-(2-aminoethoxy)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: C81 (≠ S81), G82 (= G82), A83 (≠ M83), Y107 (= Y107), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (= M213), Y334 (= Y332), S335 (= S333), W336 (= W334), R368 (= R363)
1cl1B Cystathionine beta-lyase (cbl) from escherichia coli (see paper)
46% identity, 98% coverage: 2:385/392 of query aligns to 2:391/392 of 1cl1B
4itxA P113s mutant of e. Coli cystathionine beta-lyase metc inhibited by reaction with l-ala-p (see paper)
46% identity, 98% coverage: 2:385/392 of query aligns to 1:390/391 of 4itxA
- active site: R54 (= R54), Y107 (= Y107), D181 (= D179), K206 (= K204)
- binding {1-[(3-hydroxy-methyl-5-phosphonooxy-methyl-pyridin-4-ylmethyl)-amino]-ethyl}-phosphonic acid: C81 (≠ S81), G82 (= G82), A83 (≠ M83), Y107 (= Y107), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (= M213), Y334 (= Y332), S335 (= S333), W336 (= W334), R368 (= R363)
8sabA Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp adduct with alanine (c2 form)
46% identity, 97% coverage: 4:385/392 of query aligns to 4:391/392 of 8sabA
- binding lysine: N9 (≠ H9), R12 (= R12), R13 (≠ D13), K14 (≠ P14), T17 (≠ F17), L330 (= L327), E341 (= E338)
- binding pyridoxal-5'-phosphate: C82 (≠ S81), G83 (= G82), A84 (≠ M83), Y108 (= Y107), D182 (= D179), A204 (= A201), T206 (= T203), K207 (= K204), M216 (= M213), W337 (= W334)
- binding alanyl-pyridoxal-5'-phosphate: C82 (≠ S81), G83 (= G82), A84 (≠ M83), Y108 (= Y107), D182 (= D179), A204 (= A201), T206 (= T203), K207 (= K204), M216 (= M213), Y335 (= Y332), S336 (= S333), W337 (= W334), R369 (= R363)
8u99A Crystal structure of cystathionine beta lyase from klebsiella aerogenes (plp-serine adduct)
46% identity, 97% coverage: 4:385/392 of query aligns to 3:390/391 of 8u99A
- binding pyridoxal-5'-phosphate: C81 (≠ S81), G82 (= G82), A83 (≠ M83), Y107 (= Y107), D181 (= D179), T205 (= T203), K206 (= K204), M215 (= M213), W336 (= W334)
- binding serine: Y107 (= Y107), K206 (= K204), Y334 (= Y332), S335 (= S333), W336 (= W334), R368 (= R363)
8u98A Crystal structure of cystathionine beta lyase from klebsiella aerogenes (plp-glycine adduct)
46% identity, 97% coverage: 4:385/392 of query aligns to 3:390/391 of 8u98A
- binding glycine: Y107 (= Y107), K206 (= K204), Y334 (= Y332), S335 (= S333), W336 (= W334), R368 (= R363)
- binding pyridoxal-5'-phosphate: Y52 (= Y52), R54 (= R54), C81 (≠ S81), G82 (= G82), A83 (≠ M83), Y107 (= Y107), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (= M213), W336 (= W334)
8sa9A Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp-oxamate adduct (c2 form)
46% identity, 97% coverage: 4:385/392 of query aligns to 3:390/391 of 8sa9A
- binding pyridoxal-5'-phosphate: C81 (≠ S81), G82 (= G82), A83 (≠ M83), Y107 (= Y107), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (= M213), W336 (= W334)
- binding [({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino](oxo)acetic acid: C81 (≠ S81), G82 (= G82), A83 (≠ M83), Y107 (= Y107), D181 (= D179), A203 (= A201), T205 (= T203), K206 (= K204), M215 (= M213), S335 (= S333), W336 (= W334), R368 (= R363)
8sadA Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp/malonate complex (c2 form)
46% identity, 97% coverage: 4:385/392 of query aligns to 10:397/398 of 8sadA
- binding magnesium ion: A359 (= A347), R362 (= R350), A365 (≠ T353)
- binding pyridoxal-5'-phosphate: C88 (≠ S81), G89 (= G82), A90 (≠ M83), Y114 (= Y107), D188 (= D179), A210 (= A201), T212 (= T203), K213 (= K204), M222 (= M213), W343 (= W334)
1e5eA Methionine gamma-lyase (mgl) from trichomonas vaginalis in complex with propargylglycine
35% identity, 98% coverage: 1:385/392 of query aligns to 3:392/394 of 1e5eA
- active site: R55 (= R54), Y108 (= Y107), D181 (= D179), K206 (= K204)
- binding n-(hydroxy{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)norvaline: Y53 (= Y52), R55 (= R54), G83 (= G82), M84 (= M83), Y108 (= Y107), N155 (≠ S154), D181 (= D179), S203 (≠ A201), T205 (= T203), K206 (= K204), S335 (= S333), T350 (vs. gap), R370 (= R363)
1e5fA Methionine gamma-lyase (mgl) from trichomonas vaginalis
35% identity, 98% coverage: 1:385/392 of query aligns to 3:392/393 of 1e5fA
- active site: R55 (= R54), Y108 (= Y107), D181 (= D179), K206 (= K204)
- binding pyridoxal-5'-phosphate: Y53 (= Y52), R55 (= R54), G83 (= G82), M84 (= M83), Y108 (= Y107), D181 (= D179), S203 (≠ A201), K206 (= K204)
8j6nA Crystal structure of cystathionine gamma-lyase in complex with compound 1 (see paper)
34% identity, 96% coverage: 5:382/392 of query aligns to 11:385/390 of 8j6nA
- binding [6-methyl-4-[(~{E})-(oxamoylhydrazinylidene)methyl]-5-oxidanyl-pyridin-3-yl]methyl dihydrogen phosphate: Y51 (= Y52), R53 (= R54), G81 (= G82), L82 (≠ M83), Y105 (= Y107), E148 (= E150), N152 (≠ S154), D178 (= D179), S200 (≠ A201), T202 (= T203), K203 (= K204), E330 (≠ Y332), S331 (= S333), T346 (= T344), R366 (= R363)
4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
33% identity, 97% coverage: 4:384/392 of query aligns to 9:394/396 of 4omaA
- active site: R59 (= R54), Y112 (= Y107), D184 (= D179), K209 (= K204)
- binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G82), I88 (≠ M83), Y112 (= Y107), D184 (= D179), S206 (≠ A201), T208 (= T203), K209 (= K204), V337 (≠ Y332), S338 (= S333), R373 (= R363)
3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
33% identity, 97% coverage: 4:384/392 of query aligns to 9:394/396 of 3jwbA
3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
33% identity, 97% coverage: 4:384/392 of query aligns to 9:394/396 of 3jwaA
3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
33% identity, 97% coverage: 4:384/392 of query aligns to 9:394/396 of 3jw9A
5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
33% identity, 97% coverage: 4:384/392 of query aligns to 8:393/395 of 5m3zA
- active site: R58 (= R54), Y111 (= Y107), D183 (= D179), K208 (= K204)
- binding norleucine: Y111 (= Y107), H113 (≠ P109), K208 (= K204), V336 (≠ Y332), S337 (= S333)
- binding pyridoxal-5'-phosphate: G86 (= G82), I87 (≠ M83), Y111 (= Y107), E154 (= E150), D183 (= D179), T185 (= T181), S205 (≠ A201), T207 (= T203), K208 (= K204)
- binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G82), I87 (≠ M83), Y111 (= Y107), D183 (= D179), S205 (≠ A201), T207 (= T203), K208 (= K204), V336 (≠ Y332), S337 (= S333), R372 (= R363)
6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
33% identity, 97% coverage: 4:384/392 of query aligns to 9:394/396 of 6egrA
Query Sequence
>YP_426700.1 NCBI__GCF_000013085.1:YP_426700.1
MKQDTTVVHSGRDPQRFDGVVNVPAYRASTVTFPTVADLKRKHAIRFETTYYGRYGTPTT
AALEEAVAALEGATHCAATSSGMGAVAGALLTFLKQGDHLLMVDTVYWPTRKFCDVFLKN
FGVETTYYDPLIGAGIAALIRPNTKIVYCESPGSHTFQVQDIPAIAGEAHAKGALVLLDN
TWASPLFFEPFSKGVDVSIQAATKYIVGHSDAMLGTISVRQRDWFLAIKDTLGSFGYATG
SEEAFLGLRGLRTLSARLERHQRSALAVATWLQSQPEVERVLYPALPDDPGHALWTRDFT
GASGLFGVLLKAGTEEQVAHMLDHMTLFAMGYSWGGYESLVIPTRGAIERTATQWTHQGP
SLRLHIGLEDPADLIADLRAGLDRLGEKALDQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory