SitesBLAST

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
39% identity, 79% coverage: 19:237/276 of query aligns to 5:234/304 of Q96XT4

query
sites
Q96XT4

K

K

P

P

V

V

-

F

-

V

-

D

W

W

C
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C

P

P

G

G

C
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C

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G

D

D

Y

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K

M

A

A

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E

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I

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I

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G

C
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C

S
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S

S

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K

I

I

P

P

A

H

Y

F

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M

-

N

-

L

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H

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D

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N

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N

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Y

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S

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V

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Y

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G

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x
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T

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K

G

G

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K

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C

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-

I

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-

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C12 (= C23) binding [4Fe-4S] cluster
C15 (= C26) binding [4Fe-4S] cluster
IGCS 44:47 (= IGCS 55:58) binding thiamine diphosphate
C46 (= C57) binding [4Fe-4S] cluster
D90 (= D99) binding Mg(2+)
GD 91:92 (= GD 100:101) binding thiamine diphosphate
N118 (= N127) binding Mg(2+)
V120 (= V129) binding Mg(2+)
GL 122:123 (≠ GM 131:132) binding thiamine diphosphate
C197 (= C207) binding [4Fe-4S] cluster

5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
39% identity, 79% coverage: 19:237/276 of query aligns to 2:231/301 of 5b46B

query
sites
5b46B

K

K

P

P

V

V

-

F

-

V

-

D

W
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W

C
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C

P

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G

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C
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C

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G

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I

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C
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C

S
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S

S

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H

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K

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binding magnesium ion: D87 (= D99), N115 (= N127), V117 (= V129)
binding iron/sulfur cluster: W8 (= W22), C9 (= C23), C12 (= C26), C43 (= C57), C194 (= C207), T196 (= T209), Y197 (= Y210)
binding thiamine diphosphate: I41 (= I55), G42 (= G56), C43 (= C57), S44 (= S58), H62 (= H74), G86 (= G98), G88 (= G100), D89 (= D101), N115 (= N127), V117 (= V129), Y118 (= Y130), G119 (= G131), L120 (≠ M132), T121 (= T133)

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
39% identity, 88% coverage: 22:264/276 of query aligns to 7:249/286 of 5b48B

query
sites
5b48B

W

W

C
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C

P

P

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G

C
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C

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G

D

N

Y

F

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I

G
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G

C
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C

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S

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D

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I

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W

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A

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K

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K

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P

E

E

-

E

-

V

-

N

-

E

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K

-

I

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K

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R

A

A

F

I

R

D

K

K

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L

L

D

E

D

W

D

G

G

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G

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binding magnesium ion: D84 (= D99), N112 (= N127), V114 (= V129)
binding iron/sulfur cluster: C8 (= C23), C11 (= C26), C42 (= C57), C183 (= C207), T185 (= T209)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I55), G41 (= G56), C42 (= C57), S43 (= S58), H59 (= H74), G85 (= G100), D86 (= D101), N112 (= N127), V114 (= V129), Y115 (= Y130), G116 (= G131), L117 (≠ M132)

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
38% identity, 88% coverage: 22:264/276 of query aligns to 11:267/305 of Q96Y68

query
sites
Q96Y68

W

W

C
|
C

P

P

G

G

C
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C

G

G

D

N

Y

F

S

G

V

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I

L

A

N

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A

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A

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C

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K

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G

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G

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I

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A

N

G

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A

A

A

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R

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N

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V

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D

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D

N
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N

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V

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Y

G
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G

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K
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K

G

G

Q

Q

A

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W

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K

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C
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C

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T

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N

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W

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E

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K

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A

L

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D

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K

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K

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R

C12 (= C23) binding [4Fe-4S] cluster
C15 (= C26) binding [4Fe-4S] cluster
C46 (= C57) binding [4Fe-4S] cluster
K49 (≠ R60) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D99) binding Mg(2+)
GD 91:92 (= GD 100:101) binding thiamine diphosphate
N118 (= N127) binding Mg(2+)
V120 (= V129) binding Mg(2+)
GL 122:123 (≠ GM 131:132) binding thiamine diphosphate
K125 (= K134) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (≠ G183) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C207) binding [4Fe-4S] cluster

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
38% identity, 88% coverage: 22:264/276 of query aligns to 11:267/305 of P72579

query
sites
P72579

W

W

C

C

P

P

G

G

C

C

G

G

D

N

Y

F

S

G

V

I

I

L

A

N

S

A

I

E

T

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K

Q

A

A

L

I

A

V

E

E

L

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L

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K

E

N

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V

A

V

F

V

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V

S

S

G

G

I

I

G

G

C

C

S

S

S

G

R
x
K

I

I

P

P

A

H

Y

F

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F

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R

S

T

A

P

V

I

Y

S

G

G

F

V

H

H

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L

H

H

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R

A

A

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A

I

F

A

A

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T

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G

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K

M

L

A

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P

D

D

L

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V

V

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A

N

G

G

D

D

G

G

D

D

G

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F

L

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G

I

I

G

G

G

A

N

G

H

H

F

F

M

V

H

A

A

A

C

G

R

R

N

N

V

V

D

D

L

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T

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Y

V

I

I

V

L

M

H

D

D

N

N

S

G

V

V

Y

Y

G

G

M
x
L

T

T

K

K

G

G

Q

Q

A

A

S

S

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T

T

L

E

K

A

R

D

G

W

E

G

K

G

P

S

K

K

S

L

L

-

P

T

R

P

P

N

N

G

I

T

N

G

D

L

A

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F

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N

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A

I

L

A

A

L

I

S

S

A

S

G

G

A

Y

S

T

F

F

I

V

G

A

R

R

A

G

F

Y

S

A

G

Y

Q

D

P

V

G

K

E

H

L

L

T

K

R

E

Q

L

I

I

C

K

E

S

G

A

I

I

H

K

H

H

K

K

G

G

F

L

S

A

F

L

I

I

H

D

V

V

L

L

S

Q

P

P

C

C

V

P

T

T

Y

Y

R

N

P

D

-

I

-

N

-

T

-

K

-

E

-

W

-

Y

E

D

Q

K

R

R

A

I

Y

Y

K

K

D

L

R

D

V

T

H

L

Q

P

E

D

W

W

Q

D

P

P

T

V

A

V

D

K

R

K

-

P

-

E

-

V

T

N

E

E

A

K

F

I

R

K

K

R

L

A

L

I

D

D

D

K

-

S

-

L

-

E

-

W

-

G

D

D

G

R

F

I

S

P

T

I

G

G

I

I

L

F

Y

Y

K

Q

G

N

T

-

K

-

P

-

A

E

F

L

V

V

P

P

E

S

T

Y

A

E

E

E

R

R

K49 (≠ R60) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (≠ M132) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
38% identity, 78% coverage: 22:237/276 of query aligns to 4:205/275 of 5b47B

query
sites
5b47B

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

Y

F

S

G

V

I

I

L

A

R

S

A

I

E

T

E

K

M

A

A

L

I

A

R

E

E

L

L

Q

G

L

I

V

N

P

P

E

K

E

S

V

V

A

V

F

I

V

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

R

K

I

I

P

P

A

H

Y

F

S

M

A

N

-

L

-

P

V

I

Y

S

G

G

F

V

H

H

G

T

V

L

H
|
H

G

G

R

R

A

S

L

I

P

A

I

F

A

A

T

T

G

G

L

I

K

K

M

L

A

S

R

N

Q

P

D

S

L

L

T

E

V

V

L

I

V

V

A

N

G

V

G

G

D
|
D

G
|
G

D
|
D

G

G

F

L

S

G

I

I

G

G

G

M

N

G

H

H

F

F

M

V

H

H

A

L

C

G

R

R

N

N

V

I

D

D

L

I

T

A

Y

V

I

L

V

V

M

H

D

N

N
|
N

S

G

V
|
V

Y
|
Y

G

G

M
x
L

T

-

K

-

G

-

Q

Q

A

A

S

S

P

P

T

T

T

L

E

H

A

-

D

-

W

-

G

-

S

-

K

-

L

-

T

-

P

-

N

R

G

G

T

E

G

N

L

I

L

M

-

D

P

A

F

V

Q

N

P

P

L

L

E

A

I

V

A

A

L

L

S

A

A

A

G

G

A

Y

S

T

F

F

I

V

G

A

R

R

A

G

F

Y

S

A

G

Y

Q

D

P

V

G

M

E

H

L

L

T

K

R

E

Q

L

I

I

C

K

E

K

G

A

I

I

H

L

H

H

K

K

G

G

F

S

S

A

F

L

I

V

H

D

V

I

L

L

S

Q

P

P

C
|
C

V

P

T

T

Y

Y

R

-

P

-

E

-

Q

-

R

R

A

V

Y

Y

K

K

D

L

R

D

V

N

H

V

Q

P

E

G

W

W

Q

D

P

P

T

V

A

V

D

R

R

K

T

E

E

E

A

E

F

A

R

Q

K

K

binding magnesium ion: D83 (= D99), N111 (= N127)
binding iron/sulfur cluster: C5 (= C23), C8 (= C26), C39 (= C57), C179 (= C207)
binding thiamine diphosphate: I37 (= I55), G38 (= G56), C39 (= C57), S40 (= S58), H58 (= H74), G84 (= G100), D85 (= D101), N111 (= N127), V113 (= V129), Y114 (= Y130), L116 (≠ M132)

5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
27% identity, 55% coverage: 92:244/276 of query aligns to 103:248/314 of 5exeC

query
sites
5exeC

T

N

V

I

L

I

V

V

A

M

G

A

G
|
G

D
|
D

G
|
G

D

G

G

A

F

V

S

D

I

I

G

G

G

L

N

Q

H

A

F

L

M

S

H

A

A

M

C

L

R

Y

R

R

N

G

V

H

D
|
D

L

V

T

L

Y

F

I

I

V

C

M
x
Y

D

D

N
|
N

S

E

V
x
S

Y
|
Y

G
x
A

M
x
N

T
|
T

K

G

G

I

Q

Q

A

T

S

S

P

P

T

T

E

P

A

-

D

-

W

Y

G

G

G

A

S

N

-

T

-

F

-

T

-

P

-

G

K

E

L

V

T

V

P

P

N

E

G

G

T

K

G

K

L

L

L

F

P

P

F

K

-

D

Q

N

P

P

L

K

E

V

I

I

A

A

L

H

S

G

-

H

A

P

G

E

A

L

S

K

F

Y

I

V

G

A

R

T

A

A

F

S

S

I

G

G

Q

W

P

P

G

V

E

D

L

L

T

M

R

N

Q

K

I

V

C

R

E

K

G

G

I
x
L

H

N

H
x
Q

K

E

G

G

F

P

S

A

F

Y

I

I

H

H

V

I

L

H

S

A

P

P

C
|
C

V

-

T

-

Y

-

R

-

P

-

E

-

Q

-

R

-

A

-

Y

-

K

-

D

-

R

-

V

-

H
x
P

Q
x
K

E

G

W

W

Q

Q

P

F

T

P

A

A

D

D

R

K

T

T

E

I

A

E

F

M

R

A

K

K

L

L

L

A

D

V

D

Q

D

T

G

G

F

M

active site: N143 (≠ M132)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: G109 (= G98), D110 (= D99), G111 (= G100), Y136 (≠ M125), N138 (= N127), S140 (≠ V129), Y141 (= Y130), A142 (≠ G131), N143 (≠ M132), T144 (= T133)
binding magnesium ion: D110 (= D99), D130 (= D119), N138 (= N127), S140 (≠ V129), L211 (≠ I193), Q213 (≠ H195)
binding iron/sulfur cluster: C225 (= C207), P226 (≠ H222), K227 (≠ Q223)

Sites not aligning to the query:

5c4iC Structure of an oxalate oxidoreductase (see paper)
27% identity, 55% coverage: 92:244/276 of query aligns to 103:248/312 of 5c4iC

query
sites
5c4iC

T

N

V

I

L

I

V

V

A

M

G

A

G
|
G

D
|
D

G
|
G

D

G

G

A

F

V

S

D

I

I

G

G

G

L

N

Q

H

A

F

L

M

S

H

A

A

M

C

L

R

Y

R

R

N

G

V

H

D

D

L

V

T

L

Y

F

I

I

V

C

M
x
Y

D

D

N
|
N

S

E

V
x
S

Y
|
Y

G
x
A

M
x
N

T
|
T

K

G

G

I

Q

Q

A

T

S

S

P

P

T

T

E

P

A

-

D

-

W

Y

G

G

G

A

S

N

-

T

-

F

-

T

-

P

-

G

K

E

L

V

T

V

P

P

N

E

G

G

T

K

G

K

L

L

L

F

P

P

F

K

-

D

Q

N

P

P

L

K

E

V

I

I

A

A

L

H

S

G

-

H

A

P

G

E

A

L

S

K

F

Y

I

V

G

A

R

T

A

A

F

S

S

I

G

G

Q

W

P

P

G

V

E

D

L

L

T

M

R

N

Q

K

I

V

C

R

E

K

G

G

I

L

H

N

H

Q

K

E

G

G

F

P

S

A

F

Y

I

I

H

H

V

I

L

H

S

A

P

P

C
|
C

V

-

T

-

Y

-

R

-

P

-

E

-

Q

-

R

-

A

-

Y

-

K

-

D

-

R

-

V

-

H
x
P

Q
x
K

E

G

W

W

Q

Q

P

F

T

P

A

A

D

D

R

K

T

T

E

I

A

E

F

M

R

A

K

K

L

L

L

A

D

V

D

Q

D

T

G

G

F

M

active site: N143 (≠ M132)
binding magnesium ion: D110 (= D99), N138 (= N127), S140 (≠ V129)
binding iron/sulfur cluster: A142 (≠ G131), C225 (= C207), P226 (≠ H222), K227 (≠ Q223)
binding thiamine diphosphate: G109 (= G98), D110 (= D99), G111 (= G100), Y136 (≠ M125), N138 (= N127), S140 (≠ V129), Y141 (= Y130), A142 (≠ G131), N143 (≠ M132), T144 (= T133)

Sites not aligning to the query:

binding iron/sulfur cluster: 24, 27, 29, 52
binding thiamine diphosphate: 50, 51, 52, 74

5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
27% identity, 55% coverage: 92:244/276 of query aligns to 103:248/310 of 5exdF

query
sites
5exdF

T

N

V

I

L

I

V

V

A

M

G

A

G

G

D
|
D

G
|
G

D

G

G

A

F

V

S

D

I

I

G

G

G

L

N

Q

H

A

F

L

M

S

H

A

A

M

C

L

R

Y

R

R

N

G

V

H

D

D

L

V

T

L

Y

F

I

I

V

C

M
x
Y

D

D

N
|
N

S

E

V
x
S

Y
|
Y

G
x
A

M
x
N

T
|
T

K

G

G

I

Q

Q

A

T

S

S

P

P

T

T

E

P

A

-

D

-

W

Y

G

G

G

A

S

N

-

T

-

F

-

T

-

P

-

G

K

E

L

V

T

V

P

P

N

E

G

G

T

K

G

K

L

L

L

F

P

P

F

K

-

D

Q

N

P

P

L

K

E

V

I

I

A

A

L

H

S

G

-

H

A

P

G

E

A

L

S

K

F

Y

I

V

G

A

R

T

A

A

F

S

S

I

G

G

Q

W

P

P

G

V

E

D

L

L

T

M

R

N

Q

K

I

V

C

R

E

K

G

G

I

L

H

N

H

Q

K

E

G

G

F

P

S

A

F

Y

I

I

H

H

V

I

L

H

S

A

P

P

C
|
C

V

-

T

-

Y

-

R

-

P

-

E

-

Q

-

R

-

A

-

Y

-

K

-

D

-

R

-

V

-

H

P

Q
x
K

E

G

W

W

Q

Q

P

F

T

P

A

A

D

D

R

K

T

T

E

I

A

E

F

M

R

A

K

K

L

L

L

A

D

V

D

Q

D

T

G

G

F

M

active site: N143 (≠ M132)
binding magnesium ion: D110 (= D99), N138 (= N127), S140 (≠ V129)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: D110 (= D99), G111 (= G100), Y136 (≠ M125), N138 (= N127), S140 (≠ V129), Y141 (= Y130), A142 (≠ G131), N143 (≠ M132), T144 (= T133)
binding iron/sulfur cluster: A142 (≠ G131), C225 (= C207), K227 (≠ Q223)

Sites not aligning to the query:

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
24% identity, 66% coverage: 92:274/276 of query aligns to 960:1143/1171 of Q2RMD6

query
sites
Q2RMD6

T

S

V

I

L

W

V

I

A

I

G

G

D
|
D

G
|
G

D
x
W

G

A

F

Y

S

D

I

I

G

G

G

Y

N

G

H

G

F

L

M

D

H

H

A

V

C

L

R

A

R

S

N

G

V

A

D

N

L

V

T

N

Y

V

I

L

V

V

M

L

D

D

N
x
T

S
x
E

V
|
V

Y
|
Y

G
x
S

M
x
N

T

T

K

G

G

G

Q

Q

A

S

S

S

P

K

T

A

T

T

E

Q

A

T

D

G

-

A

-

V

-

A

-

R

-

F

-

A

W

A

G

G

S

K

K

F

L

T

T

K

P

K

N

K

G

D

T

L

G

G

L

L

L

M

P

-

F

-

Q

-

P

-

L

-

E

-

I

-

A

A

L

M

S

S

A

Y

G

G

A

Y

S

V

F

Y

I

V

G

A

R

S

-

V

A

A

F

M

S

G

G

A

Q

S

P

H

G

S

E

Q

L

L

T

M

R

K

Q

A

I

L

C

I

E

E

G

A

I

E

H

K

H

Y

K

D

G

G

F

P

S

S

F

L

I

I

H

I

V

A

L

Y

S

A

P

P

C
|
C

V

I

T

N

Y

H

-

G

-

I

-

N

-

M

R

T

P

Y

E

S

Q

Q

R

R

A

E

Y

A

K

K

D

K

R

A

V

V

H

E

Q

A

E

G

W

Y

Q

W

P

P

T

L

A

Y

D

R

R

-

T

-

E

-

A

-

F

Y

R

N

K

P

L

Q

L

L

D

A

D

Q

D

E

G

G

F

K

S

N

T

P

G

F

I

I

L

L

-

D

Y

Y

K

K

G

T

T

P

K

T

P

A

A

S

F

F

V

R

P

D

E

F

T

L

A

M

E

G

R

E

A

I

S

R

F

Y

T

T

E

S

I

L

E

K

A

K

Q

Q

F

F

D967 (= D99) binding Mg(2+)
DGW 967:969 (≠ DGD 99:101) binding thiamine diphosphate
T995 (≠ N127) binding Mg(2+)
TEVYSN 995:1000 (≠ NSVYGM 127:132) binding thiamine diphosphate
V997 (= V129) binding Mg(2+)
C1075 (= C207) binding [4Fe-4S] cluster

Sites not aligning to the query:

424:428 binding CoA
456 binding CoA
556 binding CoA
598 binding CoA
686 binding [4Fe-4S] cluster
689 binding [4Fe-4S] cluster
692 binding [4Fe-4S] cluster
696 binding [4Fe-4S] cluster
742 binding [4Fe-4S] cluster
745 binding [4Fe-4S] cluster
748 binding [4Fe-4S] cluster
752 binding [4Fe-4S] cluster
809 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
814 binding thiamine diphosphate
837 binding [4Fe-4S] cluster; binding thiamine diphosphate

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
24% identity, 66% coverage: 92:274/276 of query aligns to 959:1142/1169 of 6ciqA

query
sites
6ciqA

T

S

V

I

L

W

V

I

A

I

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

S

D

I

I

G

G

G

Y

N

G

H

G

F

L

M

D

H

H

A

V

C

L

R

A

R

S

N

G

V

A

D

N

L

V

T

N

Y

V

I

L

V

V

M

L

D

D

N
x
T

S

E

V
|
V

Y
|
Y

G
x
S

M
x
N

T
|
T

K

G

G

G

Q

Q

A

S

S

S

P

K

T

A

T

T

E

Q

A

T

D

G

-

A

-

V

-

A

-

R

-

F

-

A

W

A

G

G

S

K

K

F

L

T

T

K

P

K

N

K

G

D

T

L

G

G

L

L

L

M

P

-

F

-

Q

-

P

-

L

-

E

-

I

-

A

A

L

M

S

S

A

Y

G

G

A

Y

S

V

F

Y

I

V

G

A

R

S

-

V

A

A

F

M

S

G

G

A

Q

S

P

H

G

S

E

Q

L

L

T

M

R

K

Q

A

I

L

C

I

E

E

G

A

I

E

H

K

H

Y

K

D

G

G

F

P

S

S

F

L

I

I

H

I

V

A

L

Y

S

A

P

P

C
|
C

V
x
I

T

N

Y

H

-

G

-

I

-

N

-

M

R

T

P

Y

E

S

Q

Q

R

R

A

E

Y

A

K

K

D

K

R

A

V

V

H

E

Q

A

E

G

W

Y

Q

W

P

P

T

L

A

Y

D

R

R

-

T

-

E

-

A

-

F

Y

R

N

K

P

L

Q

L

L

D

A

D

Q

D

E

G

G

F

K

S

N

T

P

G

F

I

I

L

L

-

D

Y

Y

K

K

G

T

T

P

K

T

P

A

A

S

F

F

V

R

P

D

E

F

T

L

A

M

E

G

R

E

A

I

S

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Y

T

T

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S

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L

E

K

A

K

Q

Q

F

F

active site: N999 (≠ M132)
binding coenzyme a: N999 (≠ M132)
binding magnesium ion: D966 (= D99), T994 (≠ N127), V996 (= V129)
binding iron/sulfur cluster: S998 (≠ G131), C1074 (= C207), I1075 (≠ V208)
binding thiamine diphosphate: G965 (= G98), D966 (= D99), G967 (= G100), V996 (= V129), Y997 (= Y130), S998 (≠ G131), N999 (≠ M132), T1000 (= T133)

Sites not aligning to the query:

active site: 28, 61, 111
binding coenzyme a: 28, 423, 424, 427, 428, 553, 586
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 85, 813, 835, 836, 865

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
24% identity, 66% coverage: 92:274/276 of query aligns to 959:1142/1169 of 6cinB

query
sites
6cinB

T

S

V

I

L

W

V

I

A

I

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

S

D

I

I

G

G

G

Y

N

G

H

G

F

L

M

D

H

H

A

V

C

L

R

A

R

S

N

G

V

A

D

N

L

V

T

N

Y

V

I

L

V

V

M

L

D

D

N
x
T

S

E

V
|
V

Y
|
Y

G
x
S

M
x
N

T
|
T

K

G

G

G

Q

Q

A

S

S

S

P

K

T

A

T

T

E

Q

A

T

D

G

-

A

-

V

-

A

-

R

-

F

-

A

W

A

G

G

S

K

K

F

L

T

T

K

P

K

N

K

G

D

T

L

G

G

L

L

L

M

P

-

F

-

Q

-

P

-

L

-

E

-

I

-

A

A

L

M

S

S

A

Y

G

G

A

Y

S

V

F

Y

I

V

G

A

R

S

-

V

A

A

F

M

S

G

G

A

Q

S

P

H

G

S

E

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L

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M

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K

Q

A

I

L

C

I

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E

G

A

I

E

H

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H

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K

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G

G

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S

F

L

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I

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I

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C
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C

V
x
I

T

N

Y

H

-

G

-

I

-

N

-

M

R

T

P

Y

E

S

Q

Q

R

R

A

E

Y

A

K

K

D

K

R

A

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V

H

E

Q

A

E

G

W

Y

Q

W

P

P

T

L

A

Y

D

R

R

-

T

-

E

-

A

-

F

Y

R

N

K

P

L

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L

L

D

A

D

Q

D

E

G

G

F

K

S

N

T

P

G

F

I

I

L

L

-

D

Y

Y

K

K

G

T

T

P

K

T

P

A

A

S

F

F

V

R

P

D

E

F

T

L

A

M

E

G

R

E

A

I

S

R

F

Y

T

T

E

S

I

L

E

K

A

K

Q

Q

F

F

active site: N999 (≠ M132)
binding magnesium ion: D966 (= D99), T994 (≠ N127), V996 (= V129)
binding iron/sulfur cluster: S998 (≠ G131), C1074 (= C207), I1075 (≠ V208)
binding thiamine diphosphate: G965 (= G98), D966 (= D99), G967 (= G100), V996 (= V129), Y997 (= Y130), S998 (≠ G131), N999 (≠ M132), T1000 (= T133)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 834, 835, 836, 865

6cipA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with acetyl-tpp bound (see paper)
25% identity, 43% coverage: 92:210/276 of query aligns to 959:1077/1165 of 6cipA

query
sites
6cipA

T

S

V

I

L

W

V

I

A

I

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

S

D

I

I

G

G

G

Y

N

G

H

G

F

L

M

D

H

H

A

V

C

L

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A

R

S

N

G

V

A

D

N

L

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D

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x
T

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|
V

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Y

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x
S

M
x
N

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G

G

G

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Q

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S

S

S

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T

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T

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D

G

-

A

-

V

-

A

-

R

-

F

-

A

W

A

G

G

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K

F

L

T

T

K

P

K

N

K

G

D

T

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G

G

L

L

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P

-

F

-

Q

-

P

-

L

-

E

-

I

-

A

A

L

M

S

S

A

Y

G

G

A

Y

S

V

F

Y

I

V

G

A

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-

V

A

A

F

M

S

G

G

A

Q

S

P

H

G

S

E

Q

L

L

T

M

R

K

Q

A

I

L

C

I

E

E

G

A

I

E

H

K

H

Y

K

D

G

G

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P

S

S

F

L

I

I

H

I

V

A

L

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C
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C

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I

T

N

Y

H

active site: N999 (≠ M132)
binding 2-acetyl-thiamine diphosphate: G965 (= G98), D966 (= D99), G967 (= G100), V996 (= V129), S998 (≠ G131), N999 (≠ M132)
binding magnesium ion: D966 (= D99), T994 (≠ N127), V996 (= V129)
binding iron/sulfur cluster: S998 (≠ G131), C1074 (= C207)

Sites not aligning to the query:

active site: 28, 61, 111
binding 2-acetyl-thiamine diphosphate: 27, 61, 85, 111, 813, 834, 835, 836, 865
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836

6cioA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with lactyl-tpp bound (see paper)
25% identity, 43% coverage: 92:210/276 of query aligns to 959:1077/1164 of 6cioA

query
sites
6cioA

T

S

V

I

L

W

V

I

A

I

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

S

D

I

I

G

G

G

Y

N

G

H

G

F

L

M

D

H

H

A

V

C

L

R

A

R

S

N

G

V

A

D

N

L

V

T

N

Y

V

I

L

V

V

M

L

D

D

N
x
T

S

E

V
|
V

Y
|
Y

G
x
S

M
x
N

T
|
T

K

G

G

G

Q

Q

A

S

S

S

P

K

T

A

T

T

E

Q

A

T

D

G

-

A

-

V

-

A

-

R

-

F

-

A

W

A

G

G

S

K

K

F

L

T

T

K

P

K

N

K

G

D

T

L

G

G

L

L

L

M

P

-

F

-

Q

-

P

-

L

-

E

-

I

-

A

A

L

M

S

S

A

Y

G

G

A

Y

S

V

F

Y

I

V

G

A

R

S

-

V

A

A

F

M

S

G

G

A

Q

S

P

H

G

S

E

Q

L

L

T

M

R

K

Q

A

I

L

C

I

E

E

G

A

I

E

H

K

H

Y

K

D

G

G

F

P

S

S

F

L

I

I

H

I

V

A

L

Y

S

A

P

P

C
|
C

V
x
I

T

N

Y

H

active site: N999 (≠ M132)
binding magnesium ion: D966 (= D99), T994 (≠ N127), V996 (= V129)
binding iron/sulfur cluster: C1074 (= C207), I1075 (≠ V208)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: G965 (= G98), D966 (= D99), G967 (= G100), V996 (= V129), Y997 (= Y130), S998 (≠ G131), N999 (≠ M132), T1000 (= T133)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 26, 27, 28, 61, 85, 111, 813, 834, 835, 836, 865

Query Sequence

>YP_427806.1 NCBI__GCF_000013085.1:YP_427806.1
MDTLASNEYVAQDYKSDLKPVWCPGCGDYSVIASITKALAELQLVPEEVAFVSGIGCSSR
IPAYSAVYGFHGVHGRALPIATGLKMARQDLTVLVAGGDGDGFSIGGNHFMHACRRNVDL
TYIVMDNSVYGMTKGQASPTTEADWGGSKLTPNGTGLLPFQPLEIALSAGASFIGRAFSG
QPGELTRQICEGIHHKGFSFIHVLSPCVTYRPEQRAYKDRVHQEWQPTADRTEAFRKLLD
DDGFSTGILYKGTKPAFVPETAERASFTEIEAQFAV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory