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Zoomed into a clade of 18 proteins, or see all 240 proteins. Position numbering is from BT2157.

Comment: DUF1080 is a family of hydrolases and includes putative 3-ketoglycoside hydrolases. The putative active site was identified from the structure of BT2157 (PDB:3osd). In the tree, BT2157 and other 3-ketoglycoside hydrolases are colored green, and endoglucanase THTE_1561 is in red. These members of DUF1080 were taken from MicrobesOnline (using hits to PFam PF06439.11 with 80 bits or higher), clustered at 60% identity to reduce the number of sequences, and aligned using hmmalign.

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Highlight proteins:

7376920_669_882: with redox domain, from Methylobacterium nodulans ORS 2060 (has NEQ) 5653674_1059_1234: with redox domain, from Streptomyces griseus subsp. griseus NBRC 13350 (has NEQ) 1125827_671_892: with redox domain, from Nitrosospira multiformis ATCC 25196 (has NEQ) 1126194_16_255: single domain, from Nitrosospira multiformis ATCC 25196 (has NEQ) 7409619_978_1192: with redox domain, from Saccharomonospora viridis DSM 43017 (has NEQ) 246875_521_727: with other domain, from Streptomyces coelicolor A3(2) (has NEQ) 3653714_122_335: single domain, from Saccharopolyspora erythraea NRRL 2338 (has NEQ) 5593361_25_197: single domain, from Rhodospirillum centenum SW (has NEQ) 3772626_642_829: with redox domain, from Microcystis aeruginosa NIES-843 (has NEQ) 4904579_682_860: with redox domain, from Gemmata obscuriglobus UQM 2246 (has NEQ) 6252404_31_203: single domain, from Chthoniobacter flavus Ellin428 (has NED) 2225943_20_213: single domain, from Solibacter usitatus Ellin6076 (has NEQ) 11176218_20_201: single domain, from Gillisia limnaea R-8282, DSM 15749 (has NEQ) 4594548_352_539: with other domain, near sugar dehydrogenase, from Planctomyces maris DSM 8797 (has NEQ) 4614230_39_205: single domain, from Lentisphaera araneosa HTCC2155 (has NEQ) 6253107_21_166: single domain, from Chthoniobacter flavus Ellin428 (has NEQ) 11294916_26_207: single domain, from Planctomyces brasiliensis DSM 5305 (has NEQ) 2224519_22_203: single domain, from Solibacter usitatus Ellin6076 (has NQQ) BT2157 has N at position 134 (alignment position 385)N134 7376920_669_882 has N62 EQ 5653674_1059_1234 has N60 EQ 1125827_671_892 has N71 EQ 1126194_16_255 has N69 EQ 7409619_978_1192 has N68 EQ 246875_521_727 has N71 EQ 3653714_122_335 has N72 EQ 5593361_25_197 has N55 EQ 3772626_642_829 has N64 EQ 4904579_682_860 has N32 EQ 6252404_31_203 has N66 ED 2225943_20_213 has N70 EQ 11176218_20_201 has N61 EQ 4594548_352_539 has N63 EQ 4614230_39_205 has N42 EQ 6253107_21_166 has N16 EQ 11294916_26_207 has N69 EQ 2224519_22_203 has N76 QQ 7376920_669_882 and 17 similar proteins have N aligning to 134N BT2157 has E at position 164 (alignment position 438)E164 7376920_669_882 has N E98 Q 5653674_1059_1234 has N E82 Q 1125827_671_892 has N E107 Q 1126194_16_255 has N E112 Q 7409619_978_1192 has N E110 Q 246875_521_727 has N E100 Q 3653714_122_335 has N E105 Q 5593361_25_197 has N E78 Q 3772626_642_829 has N E85 Q 4904579_682_860 has N E56 Q 6252404_31_203 has N E89 D 2225943_20_213 has N E93 Q 11176218_20_201 has N E84 Q 4594548_352_539 has N E82 Q 4614230_39_205 has N E60 Q 6253107_21_166 has N E34 Q 11294916_26_207 has N E88 Q 2224519_22_203 has N Q92 Q 7376920_669_882 and 12 similar proteins have E aligning to 164E 4594548_352_539 and 2 similar proteins have E aligning to 164E 11294916_26_207 has E88 aligning to 164E 2224519_22_203 has Q92 aligning to 164Q BT2157 has Q at position 166 (alignment position 440)Q166 7376920_669_882 has NE Q100 5653674_1059_1234 has NE Q84 1125827_671_892 has NE Q109 1126194_16_255 has NE Q114 7409619_978_1192 has NE Q112 246875_521_727 has NE Q102 3653714_122_335 has NE Q107 5593361_25_197 has NE Q80 3772626_642_829 has NE Q87 4904579_682_860 has NE Q58 6252404_31_203 has NE D91 2225943_20_213 has NE Q95 11176218_20_201 has NE Q86 4594548_352_539 has NE Q84 4614230_39_205 has NE Q62 6253107_21_166 has NE Q36 11294916_26_207 has NE Q90 2224519_22_203 has NQ Q94 7376920_669_882 and 9 similar proteins have Q aligning to 166Q 6252404_31_203 has D91 aligning to 166D 2225943_20_213 and 1 similar proteins have Q aligning to 166Q 4594548_352_539 and 4 similar proteins have Q aligning to 166Q 0.891 0.549 0.053 0.880 0.347 7376920_669_882: with redox domain, from Methylobacterium nodulans ORS 2060 7376920_669_8827376920_669_882: with redox domain, from Methylobacterium nodulans ORS 2060 5653674_1059_1234: with redox domain, from Streptomyces griseus subsp. griseus NBRC 13350 5653674_1059_12345653674_1059_1234: with redox domain, from Streptomyces griseus subsp. griseus NBRC 13350 0.924 0.981 1125827_671_892: with redox domain, from Nitrosospira multiformis ATCC 25196 1125827_671_8921125827_671_892: with redox domain, from Nitrosospira multiformis ATCC 25196 1126194_16_255: single domain, from Nitrosospira multiformis ATCC 25196 1126194_16_2551126194_16_255: single domain, from Nitrosospira multiformis ATCC 25196 0.994 7409619_978_1192: with redox domain, from Saccharomonospora viridis DSM 43017 7409619_978_11927409619_978_1192: with redox domain, from Saccharomonospora viridis DSM 43017 0.949 246875_521_727: with other domain, from Streptomyces coelicolor A3(2) 246875_521_727246875_521_727: with other domain, from Streptomyces coelicolor A3(2) 3653714_122_335: single domain, from Saccharopolyspora erythraea NRRL 2338 3653714_122_3353653714_122_335: single domain, from Saccharopolyspora erythraea NRRL 2338 0.903 5593361_25_197: single domain, from Rhodospirillum centenum SW 5593361_25_1975593361_25_197: single domain, from Rhodospirillum centenum SW 0.996 3772626_642_829: with redox domain, from Microcystis aeruginosa NIES-843 3772626_642_8293772626_642_829: with redox domain, from Microcystis aeruginosa NIES-843 4904579_682_860: with redox domain, from Gemmata obscuriglobus UQM 2246 4904579_682_8604904579_682_860: with redox domain, from Gemmata obscuriglobus UQM 2246 6252404_31_203: single domain, from Chthoniobacter flavus Ellin428 6252404_31_2036252404_31_203: single domain, from Chthoniobacter flavus Ellin428 1.000 2225943_20_213: single domain, from Solibacter usitatus Ellin6076 2225943_20_2132225943_20_213: single domain, from Solibacter usitatus Ellin6076 11176218_20_201: single domain, from Gillisia limnaea R-8282, DSM 15749 11176218_20_20111176218_20_201: single domain, from Gillisia limnaea R-8282, DSM 15749 0.700 0.973 4594548_352_539: with other domain, near sugar dehydrogenase, from Planctomyces maris DSM 8797 4594548_352_5394594548_352_539: with other domain, near sugar dehydrogenase, from Planctomyces maris DSM 8797 0.793 4614230_39_205: single domain, from Lentisphaera araneosa HTCC2155 4614230_39_2054614230_39_205: single domain, from Lentisphaera araneosa HTCC2155 6253107_21_166: single domain, from Chthoniobacter flavus Ellin428 6253107_21_1666253107_21_166: single domain, from Chthoniobacter flavus Ellin428 0.899 11294916_26_207: single domain, from Planctomyces brasiliensis DSM 5305 11294916_26_20711294916_26_207: single domain, from Planctomyces brasiliensis DSM 5305 2224519_22_203: single domain, from Solibacter usitatus Ellin6076 2224519_22_2032224519_22_203: single domain, from Solibacter usitatus Ellin6076 7376920_669_882: with redox domain, from Methylobacterium nodulans ORS 2060 (has NEQ)7376920_669_882 with redox domain, from Methylobacterium nodulans ORS 2060 5653674_1059_1234: with redox domain, from Streptomyces griseus subsp. griseus NBRC 13350 (has NEQ)5653674_1059_1234 with redox domain, from Streptomyces griseus subsp. griseus NBRC 13350 1125827_671_892: with redox domain, from Nitrosospira multiformis ATCC 25196 (has NEQ)1125827_671_892 with redox domain, from Nitrosospira multiformis ATCC 25196 1126194_16_255: single domain, from Nitrosospira multiformis ATCC 25196 (has NEQ)1126194_16_255 single domain, from Nitrosospira multiformis ATCC 25196 7409619_978_1192: with redox domain, from Saccharomonospora viridis DSM 43017 (has NEQ)7409619_978_1192 with redox domain, from Saccharomonospora viridis DSM 43017 246875_521_727: with other domain, from Streptomyces coelicolor A3(2) (has NEQ)246875_521_727 with other domain, from Streptomyces coelicolor A3(2) 3653714_122_335: single domain, from Saccharopolyspora erythraea NRRL 2338 (has NEQ)3653714_122_335 single domain, from Saccharopolyspora erythraea NRRL 2338 5593361_25_197: single domain, from Rhodospirillum centenum SW (has NEQ)5593361_25_197 single domain, from Rhodospirillum centenum SW 3772626_642_829: with redox domain, from Microcystis aeruginosa NIES-843 (has NEQ)3772626_642_829 with redox domain, from Microcystis aeruginosa NIES-843 4904579_682_860: with redox domain, from Gemmata obscuriglobus UQM 2246 (has NEQ)4904579_682_860 with redox domain, from Gemmata obscuriglobus UQM 2246 6252404_31_203: single domain, from Chthoniobacter flavus Ellin428 (has NED)6252404_31_203 single domain, from Chthoniobacter flavus Ellin428 2225943_20_213: single domain, from Solibacter usitatus Ellin6076 (has NEQ)2225943_20_213 single domain, from Solibacter usitatus Ellin6076 11176218_20_201: single domain, from Gillisia limnaea R-8282, DSM 15749 (has NEQ)11176218_20_201 single domain, from Gillisia limnaea R-8282, DSM 15749 4594548_352_539: with other domain, near sugar dehydrogenase, from Planctomyces maris DSM 8797 (has NEQ)4594548_352_539 with other domain, near sugar dehydrogenase, from Planctomyces maris DSM 8797 4614230_39_205: single domain, from Lentisphaera araneosa HTCC2155 (has NEQ)4614230_39_205 single domain, from Lentisphaera araneosa HTCC2155 6253107_21_166: single domain, from Chthoniobacter flavus Ellin428 (has NEQ)6253107_21_166 single domain, from Chthoniobacter flavus Ellin428 11294916_26_207: single domain, from Planctomyces brasiliensis DSM 5305 (has NEQ)11294916_26_207 single domain, from Planctomyces brasiliensis DSM 5305 2224519_22_203: single domain, from Solibacter usitatus Ellin6076 (has NQQ)2224519_22_203 single domain, from Solibacter usitatus Ellin6076 1 /site amino acids:gapgap-alaninealanineAcysteinecysteineCaspartateaspartateDglutamateglutamateEphenylalaninephenylalanineFglycineglycineGhistidinehistidineHisoleucineisoleucineIlysinelysineKleucineleucineLmethioninemethionineMasparagineasparagineNprolineprolinePglutamineglutamineQargininearginineRserineserineSthreoninethreonineTvalinevalineVtryptophantryptophanWtyrosinetyrosineY download SVG

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory