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Zoomed into a clade of 19 proteins, or see all 240 proteins. Position numbering is from BT2157.

Comment: DUF1080 is a family of hydrolases and includes putative 3-ketoglycoside hydrolases. The putative active site was identified from the structure of BT2157 (PDB:3osd). In the tree, BT2157 and other 3-ketoglycoside hydrolases are colored green, and endoglucanase THTE_1561 is in red. These members of DUF1080 were taken from MicrobesOnline (using hits to PFam PF06439.11 with 80 bits or higher), clustered at 60% identity to reduce the number of sequences, and aligned using hmmalign.

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Highlight proteins:

558057_260_435: tandem domain, from Pirellula sp. 1 (has NEQ) 10307912_359_538: tandem domain, from Pirellula staleyi DSM 6068 (has NEQ) 10262249_29_210: single domain, from Spirosoma linguale DSM 74 (has NEQ) 4591527_43_215: single domain, from Planctomyces maris DSM 8797 (has NEQ) 2223153_25_190: single domain, from Solibacter usitatus Ellin6076 (has NEQ) 4908135_36_246: single domain, from Gemmata obscuriglobus UQM 2246 (has NEQ) 554351_85_257: single domain, from Pirellula sp. 1 (has NEQ) 11269824_44_215: single domain, from Isosphaera pallida IS1B, ATCC 43644 (has NEQ) 6253341_25_206: single domain, from Chthoniobacter flavus Ellin428 (has NEQ) 2764129_27_207: single domain, from Blastopirellula marina DSM 3645 (has NEQ) 6004371_23_210: single domain, from Verrucomicrobium spinosum DSM 4136 (has NEQ) 2221107_28_212: with other domain, from Solibacter usitatus Ellin6076 (has NEQ) 5646913_28_225: with other domain, from Opitutus terrae PB90-1 (has NEQ) 11272068_67_249: single domain, from Isosphaera pallida IS1B, ATCC 43644 (has NEQ) 3722502_28_193: with other domain, from Parabacteroides distasonis ATCC 8503 (has KRA) 4262859_41_193: with other domain, from Bacteroides uniformis ATCC 8492 (has EEI) 11292681_28_217: single domain, from Planctomyces brasiliensis DSM 5305 (has NEQ) 2221152_22_224: single domain, from Solibacter usitatus Ellin6076 (has NEQ) 2222289_283_466: with redox domain, from Solibacter usitatus Ellin6076 (has NEQ) BT2157 has N at position 134 (alignment position 385)N134 558057_260_435 has N57 EQ 10307912_359_538 has N61 EQ 10262249_29_210 has N67 EQ 4591527_43_215 has N60 EQ 2223153_25_190 has N62 EQ 4908135_36_246 has N97 EQ 554351_85_257 has N63 EQ 11269824_44_215 has N68 EQ 6253341_25_206 has N66 EQ 2764129_27_207 has N64 EQ 6004371_23_210 has N67 EQ 2221107_28_212 has N61 EQ 5646913_28_225 has N66 EQ 11272068_67_249 has N67 EQ 3722502_28_193 has K52 RA 4262859_41_193 has E44 EI 11292681_28_217 has N69 EQ 2221152_22_224 has N85 EQ 2222289_283_466 has N67 EQ 558057_260_435 and 1 similar proteins have N aligning to 134N 10262249_29_210 and 10 similar proteins have N aligning to 134N 11272068_67_249 has N67 aligning to 134N 3722502_28_193 has K52 aligning to 134K 4262859_41_193 has E44 aligning to 134E 11292681_28_217 has N69 aligning to 134N 2221152_22_224 and 1 similar proteins have N aligning to 134N BT2157 has E at position 164 (alignment position 438)E164 558057_260_435 has N E74 Q 10307912_359_538 has N E78 Q 10262249_29_210 has N E87 Q 4591527_43_215 has N E80 Q 2223153_25_190 has N E80 Q 4908135_36_246 has N E115 Q 554351_85_257 has N E81 Q 11269824_44_215 has N E86 Q 6253341_25_206 has N E86 Q 2764129_27_207 has N E84 Q 6004371_23_210 has N E87 Q 2221107_28_212 has N E81 Q 5646913_28_225 has N E87 Q 11272068_67_249 has N E86 Q 3722502_28_193 has K R68 A 4262859_41_193 has E E58 I 11292681_28_217 has N E91 Q 2221152_22_224 has N E116 Q 2222289_283_466 has N E97 Q 558057_260_435 and 1 similar proteins have E aligning to 164E 10262249_29_210 and 10 similar proteins have E aligning to 164E 11272068_67_249 has E86 aligning to 164E 3722502_28_193 has R68 aligning to 164R 4262859_41_193 has E58 aligning to 164E 11292681_28_217 has E91 aligning to 164E 2221152_22_224 and 1 similar proteins have E aligning to 164E BT2157 has Q at position 166 (alignment position 440)Q166 558057_260_435 has NE Q76 10307912_359_538 has NE Q80 10262249_29_210 has NE Q89 4591527_43_215 has NE Q82 2223153_25_190 has NE Q82 4908135_36_246 has NE Q117 554351_85_257 has NE Q83 11269824_44_215 has NE Q88 6253341_25_206 has NE Q88 2764129_27_207 has NE Q86 6004371_23_210 has NE Q89 2221107_28_212 has NE Q83 5646913_28_225 has NE Q89 11272068_67_249 has NE Q88 3722502_28_193 has KR A70 4262859_41_193 has EE I60 11292681_28_217 has NE Q93 2221152_22_224 has NE Q118 2222289_283_466 has NE Q99 558057_260_435 and 1 similar proteins have Q aligning to 166Q 10262249_29_210 and 10 similar proteins have Q aligning to 166Q 11272068_67_249 has Q88 aligning to 166Q 3722502_28_193 has A70 aligning to 166A 4262859_41_193 has I60 aligning to 166I 11292681_28_217 has Q93 aligning to 166Q 2221152_22_224 and 1 similar proteins have Q aligning to 166Q 0.629 0.998 558057_260_435: tandem domain, from Pirellula sp. 1 558057_260_435558057_260_435: tandem domain, from Pirellula sp. 1 10307912_359_538: tandem domain, from Pirellula staleyi DSM 6068 10307912_359_53810307912_359_538: tandem domain, from Pirellula staleyi DSM 6068 0.910 0.034 0.771 0.911 10262249_29_210: single domain, from Spirosoma linguale DSM 74 10262249_29_21010262249_29_210: single domain, from Spirosoma linguale DSM 74 0.455 0.672 4591527_43_215: single domain, from Planctomyces maris DSM 8797 4591527_43_2154591527_43_215: single domain, from Planctomyces maris DSM 8797 0.993 2223153_25_190: single domain, from Solibacter usitatus Ellin6076 2223153_25_1902223153_25_190: single domain, from Solibacter usitatus Ellin6076 0.910 4908135_36_246: single domain, from Gemmata obscuriglobus UQM 2246 4908135_36_2464908135_36_246: single domain, from Gemmata obscuriglobus UQM 2246 0.878 554351_85_257: single domain, from Pirellula sp. 1 554351_85_257554351_85_257: single domain, from Pirellula sp. 1 11269824_44_215: single domain, from Isosphaera pallida IS1B, ATCC 43644 11269824_44_21511269824_44_215: single domain, from Isosphaera pallida IS1B, ATCC 43644 0.974 6253341_25_206: single domain, from Chthoniobacter flavus Ellin428 6253341_25_2066253341_25_206: single domain, from Chthoniobacter flavus Ellin428 0.905 2764129_27_207: single domain, from Blastopirellula marina DSM 3645 2764129_27_2072764129_27_207: single domain, from Blastopirellula marina DSM 3645 6004371_23_210: single domain, from Verrucomicrobium spinosum DSM 4136 6004371_23_2106004371_23_210: single domain, from Verrucomicrobium spinosum DSM 4136 0.613 2221107_28_212: with other domain, from Solibacter usitatus Ellin6076 2221107_28_2122221107_28_212: with other domain, from Solibacter usitatus Ellin6076 5646913_28_225: with other domain, from Opitutus terrae PB90-1 5646913_28_2255646913_28_225: with other domain, from Opitutus terrae PB90-1 0.594 11272068_67_249: single domain, from Isosphaera pallida IS1B, ATCC 43644 11272068_67_24911272068_67_249: single domain, from Isosphaera pallida IS1B, ATCC 43644 0.974 3722502_28_193: with other domain, from Parabacteroides distasonis ATCC 8503 3722502_28_1933722502_28_193: with other domain, from Parabacteroides distasonis ATCC 8503 4262859_41_193: with other domain, from Bacteroides uniformis ATCC 8492 4262859_41_1934262859_41_193: with other domain, from Bacteroides uniformis ATCC 8492 11292681_28_217: single domain, from Planctomyces brasiliensis DSM 5305 11292681_28_21711292681_28_217: single domain, from Planctomyces brasiliensis DSM 5305 0.998 2221152_22_224: single domain, from Solibacter usitatus Ellin6076 2221152_22_2242221152_22_224: single domain, from Solibacter usitatus Ellin6076 2222289_283_466: with redox domain, from Solibacter usitatus Ellin6076 2222289_283_4662222289_283_466: with redox domain, from Solibacter usitatus Ellin6076 558057_260_435: tandem domain, from Pirellula sp. 1 (has NEQ)558057_260_435 tandem domain, from Pirellula sp. 1 10307912_359_538: tandem domain, from Pirellula staleyi DSM 6068 (has NEQ)10307912_359_538 tandem domain, from Pirellula staleyi DSM 6068 10262249_29_210: single domain, from Spirosoma linguale DSM 74 (has NEQ)10262249_29_210 single domain, from Spirosoma linguale DSM 74 4591527_43_215: single domain, from Planctomyces maris DSM 8797 (has NEQ)4591527_43_215 single domain, from Planctomyces maris DSM 8797 2223153_25_190: single domain, from Solibacter usitatus Ellin6076 (has NEQ)2223153_25_190 single domain, from Solibacter usitatus Ellin6076 4908135_36_246: single domain, from Gemmata obscuriglobus UQM 2246 (has NEQ)4908135_36_246 single domain, from Gemmata obscuriglobus UQM 2246 554351_85_257: single domain, from Pirellula sp. 1 (has NEQ)554351_85_257 single domain, from Pirellula sp. 1 11269824_44_215: single domain, from Isosphaera pallida IS1B, ATCC 43644 (has NEQ)11269824_44_215 single domain, from Isosphaera pallida IS1B, ATCC 43644 6253341_25_206: single domain, from Chthoniobacter flavus Ellin428 (has NEQ)6253341_25_206 single domain, from Chthoniobacter flavus Ellin428 2764129_27_207: single domain, from Blastopirellula marina DSM 3645 (has NEQ)2764129_27_207 single domain, from Blastopirellula marina DSM 3645 6004371_23_210: single domain, from Verrucomicrobium spinosum DSM 4136 (has NEQ)6004371_23_210 single domain, from Verrucomicrobium spinosum DSM 4136 2221107_28_212: with other domain, from Solibacter usitatus Ellin6076 (has NEQ)2221107_28_212 with other domain, from Solibacter usitatus Ellin6076 5646913_28_225: with other domain, from Opitutus terrae PB90-1 (has NEQ)5646913_28_225 with other domain, from Opitutus terrae PB90-1 11272068_67_249: single domain, from Isosphaera pallida IS1B, ATCC 43644 (has NEQ)11272068_67_249 single domain, from Isosphaera pallida IS1B, ATCC 43644 3722502_28_193: with other domain, from Parabacteroides distasonis ATCC 8503 (has KRA)3722502_28_193 with other domain, from Parabacteroides distasonis ATCC 8503 4262859_41_193: with other domain, from Bacteroides uniformis ATCC 8492 (has EEI)4262859_41_193 with other domain, from Bacteroides uniformis ATCC 8492 11292681_28_217: single domain, from Planctomyces brasiliensis DSM 5305 (has NEQ)11292681_28_217 single domain, from Planctomyces brasiliensis DSM 5305 2221152_22_224: single domain, from Solibacter usitatus Ellin6076 (has NEQ)2221152_22_224 single domain, from Solibacter usitatus Ellin6076 2222289_283_466: with redox domain, from Solibacter usitatus Ellin6076 (has NEQ)2222289_283_466 with redox domain, from Solibacter usitatus Ellin6076 1 /site amino acids:gapgap-alaninealanineAcysteinecysteineCaspartateaspartateDglutamateglutamateEphenylalaninephenylalanineFglycineglycineGhistidinehistidineHisoleucineisoleucineIlysinelysineKleucineleucineLmethioninemethionineMasparagineasparagineNprolineprolinePglutamineglutamineQargininearginineRserineserineSthreoninethreonineTvalinevalineVtryptophantryptophanWtyrosinetyrosineY download SVG

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory