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Zoomed into a clade of 12 proteins, or see all 240 proteins. Position numbering is from BT2157.

Comment: DUF1080 is a family of hydrolases and includes putative 3-ketoglycoside hydrolases. The putative active site was identified from the structure of BT2157 (PDB:3osd). In the tree, BT2157 and other 3-ketoglycoside hydrolases are colored green, and endoglucanase THTE_1561 is in red. These members of DUF1080 were taken from MicrobesOnline (using hits to PFam PF06439.11 with 80 bits or higher), clustered at 60% identity to reduce the number of sequences, and aligned using hmmalign.

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Highlight proteins:

4907078_48_238: single domain, from Gemmata obscuriglobus UQM 2246 (has NEQ) 4592216_152_332: single domain, from Planctomyces maris DSM 8797 (has NEQ) 6253284_153_334: single domain, from Chthoniobacter flavus Ellin428 (has NEQ) 557004_164_355: single domain, near sugar dehydrogenase, from Pirellula sp. 1 (has NEQ) 4612146_159_339: single domain, from Lentisphaera araneosa HTCC2155 (has NEQ) 10307987_154_329: single domain, from Pirellula staleyi DSM 6068 (has NEQ) 4616236_34_216: single domain, from Lentisphaera araneosa HTCC2155 (has NEQ) 557181_269_442: single domain, from Pirellula sp. 1 (has NEQ) 2221624_159_350: single domain, from Solibacter usitatus Ellin6076 (has NEN) 7460876_127_321: with other domain, near sugar dehydrogenase, from Pedobacter heparinus DSM 2366 (has NEN) 554434_59_259: single domain, from Pirellula sp. 1 (has NEQ) 1937924_811_999: with other domain, from Streptomyces coelicolor A3(2) (has NEQ) BT2157 has N at position 134 (alignment position 385)N134 4907078_48_238 has N72 EQ 4592216_152_332 has N62 EQ 6253284_153_334 has N64 EQ 557004_164_355 has N59 EQ 4612146_159_339 has N63 EQ 10307987_154_329 has N57 EQ 4616236_34_216 has N63 EQ 557181_269_442 has N56 EQ 2221624_159_350 has N70 EN 7460876_127_321 has N71 EN 554434_59_259 has N79 EQ 1937924_811_999 has N69 EQ 4907078_48_238 and 11 similar proteins have N aligning to 134N BT2157 has E at position 164 (alignment position 438)E164 4907078_48_238 has N E82 Q 4592216_152_332 has N E72 Q 6253284_153_334 has N E74 Q 557004_164_355 has N E69 Q 4612146_159_339 has N E73 Q 10307987_154_329 has N E67 Q 4616236_34_216 has N E73 Q 557181_269_442 has N E66 Q 2221624_159_350 has N E80 N 7460876_127_321 has N E81 N 554434_59_259 has N E89 Q 1937924_811_999 has N E79 Q 4907078_48_238 and 11 similar proteins have E aligning to 164E BT2157 has Q at position 166 (alignment position 440)Q166 4907078_48_238 has NE Q84 4592216_152_332 has NE Q74 6253284_153_334 has NE Q76 557004_164_355 has NE Q71 4612146_159_339 has NE Q75 10307987_154_329 has NE Q69 4616236_34_216 has NE Q75 557181_269_442 has NE Q68 2221624_159_350 has NE N82 7460876_127_321 has NE N83 554434_59_259 has NE Q91 1937924_811_999 has NE Q81 4907078_48_238 and 7 similar proteins have Q aligning to 166Q 2221624_159_350 and 1 similar proteins have N aligning to 166N 554434_59_259 has Q91 aligning to 166Q 1937924_811_999 has Q81 aligning to 166Q 0.421 0.541 0.473 4907078_48_238: single domain, from Gemmata obscuriglobus UQM 2246 4907078_48_2384907078_48_238: single domain, from Gemmata obscuriglobus UQM 2246 0.909 0.890 4592216_152_332: single domain, from Planctomyces maris DSM 8797 4592216_152_3324592216_152_332: single domain, from Planctomyces maris DSM 8797 6253284_153_334: single domain, from Chthoniobacter flavus Ellin428 6253284_153_3346253284_153_334: single domain, from Chthoniobacter flavus Ellin428 0.213 0.802 557004_164_355: single domain, near sugar dehydrogenase, from Pirellula sp. 1 557004_164_355557004_164_355: single domain, near sugar dehydrogenase, from Pirellula sp. 1 4612146_159_339: single domain, from Lentisphaera araneosa HTCC2155 4612146_159_3394612146_159_339: single domain, from Lentisphaera araneosa HTCC2155 0.880 0.840 10307987_154_329: single domain, from Pirellula staleyi DSM 6068 10307987_154_32910307987_154_329: single domain, from Pirellula staleyi DSM 6068 4616236_34_216: single domain, from Lentisphaera araneosa HTCC2155 4616236_34_2164616236_34_216: single domain, from Lentisphaera araneosa HTCC2155 557181_269_442: single domain, from Pirellula sp. 1 557181_269_442557181_269_442: single domain, from Pirellula sp. 1 0.970 2221624_159_350: single domain, from Solibacter usitatus Ellin6076 2221624_159_3502221624_159_350: single domain, from Solibacter usitatus Ellin6076 7460876_127_321: with other domain, near sugar dehydrogenase, from Pedobacter heparinus DSM 2366 7460876_127_3217460876_127_321: with other domain, near sugar dehydrogenase, from Pedobacter heparinus DSM 2366 554434_59_259: single domain, from Pirellula sp. 1 554434_59_259554434_59_259: single domain, from Pirellula sp. 1 1937924_811_999: with other domain, from Streptomyces coelicolor A3(2) 1937924_811_9991937924_811_999: with other domain, from Streptomyces coelicolor A3(2) 4907078_48_238: single domain, from Gemmata obscuriglobus UQM 2246 (has NEQ)4907078_48_238 single domain, from Gemmata obscuriglobus UQM 2246 4592216_152_332: single domain, from Planctomyces maris DSM 8797 (has NEQ)4592216_152_332 single domain, from Planctomyces maris DSM 8797 6253284_153_334: single domain, from Chthoniobacter flavus Ellin428 (has NEQ)6253284_153_334 single domain, from Chthoniobacter flavus Ellin428 557004_164_355: single domain, near sugar dehydrogenase, from Pirellula sp. 1 (has NEQ)557004_164_355 single domain, near sugar dehydrogenase, from Pirellula sp. 1 4612146_159_339: single domain, from Lentisphaera araneosa HTCC2155 (has NEQ)4612146_159_339 single domain, from Lentisphaera araneosa HTCC2155 10307987_154_329: single domain, from Pirellula staleyi DSM 6068 (has NEQ)10307987_154_329 single domain, from Pirellula staleyi DSM 6068 4616236_34_216: single domain, from Lentisphaera araneosa HTCC2155 (has NEQ)4616236_34_216 single domain, from Lentisphaera araneosa HTCC2155 557181_269_442: single domain, from Pirellula sp. 1 (has NEQ)557181_269_442 single domain, from Pirellula sp. 1 2221624_159_350: single domain, from Solibacter usitatus Ellin6076 (has NEN)2221624_159_350 single domain, from Solibacter usitatus Ellin6076 7460876_127_321: with other domain, near sugar dehydrogenase, from Pedobacter heparinus DSM 2366 (has NEN)7460876_127_321 with other domain, near sugar dehydrogenase, from Pedobacter heparinus DSM 2366 554434_59_259: single domain, from Pirellula sp. 1 (has NEQ)554434_59_259 single domain, from Pirellula sp. 1 1937924_811_999: with other domain, from Streptomyces coelicolor A3(2) (has NEQ)1937924_811_999 with other domain, from Streptomyces coelicolor A3(2) 1 /site amino acids:gapgap-alaninealanineAcysteinecysteineCaspartateaspartateDglutamateglutamateEphenylalaninephenylalanineFglycineglycineGhistidinehistidineHisoleucineisoleucineIlysinelysineKleucineleucineLmethioninemethionineMasparagineasparagineNprolineprolinePglutamineglutamineQargininearginineRserineserineSthreoninethreonineTvalinevalineVtryptophantryptophanWtyrosinetyrosineY download SVG

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory