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Zoomed into a clade of 6 proteins, or see all 240 proteins. Position numbering is from BT2157.

Comment: DUF1080 is a family of hydrolases and includes putative 3-ketoglycoside hydrolases. The putative active site was identified from the structure of BT2157 (PDB:3osd). In the tree, BT2157 and other 3-ketoglycoside hydrolases are colored green, and endoglucanase THTE_1561 is in red. These members of DUF1080 were taken from MicrobesOnline (using hits to PFam PF06439.11 with 80 bits or higher), clustered at 60% identity to reduce the number of sequences, and aligned using hmmalign.

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Highlight proteins:

7398304_63_256: single domain, from Dyadobacter fermentans DSM 18053 (has NEQ) 3723559_59_253: single domain, near sugar dehydrogenase, from Parabacteroides distasonis ATCC 8503 (has NEQ) 5848954_85_286: single domain, from Caulobacter crescentus NA1000 (has NEQ) Pedo557__CA265_RS22975: single domain 3-ketotrehalose hydrolase from Pedobacter sp. GW460-11-11-14-LB5 (has NEQ) 11045354_54_254: single domain, from Hymenobacter roseosalivarius AA-718, DSM 11622 (has NEQ) 11293044_51_238: single domain, from Planctomyces brasiliensis DSM 5305 (has NEQ) BT2157 has N at position 134 (alignment position 385)N134 7398304_63_256 has N68 EQ 3723559_59_253 has N72 EQ 5848954_85_286 has N70 EQ Pedo557__CA265_RS22975 has N126 EQ 11045354_54_254 has N69 EQ 11293044_51_238 has N64 EQ 7398304_63_256 and 5 similar proteins have N aligning to 134N BT2157 has E at position 164 (alignment position 438)E164 7398304_63_256 has N E78 Q 3723559_59_253 has N E82 Q 5848954_85_286 has N E86 Q Pedo557__CA265_RS22975 has N E142 Q 11045354_54_254 has N E85 Q 11293044_51_238 has N E74 Q 7398304_63_256 and 5 similar proteins have E aligning to 164E BT2157 has Q at position 166 (alignment position 440)Q166 7398304_63_256 has NE Q80 3723559_59_253 has NE Q84 5848954_85_286 has NE Q88 Pedo557__CA265_RS22975 has NE Q144 11045354_54_254 has NE Q87 11293044_51_238 has NE Q76 7398304_63_256 and 5 similar proteins have Q aligning to 166Q 0.876 7398304_63_256: single domain, from Dyadobacter fermentans DSM 18053 7398304_63_2567398304_63_256: single domain, from Dyadobacter fermentans DSM 18053 0.886 3723559_59_253: single domain, near sugar dehydrogenase, from Parabacteroides distasonis ATCC 8503 3723559_59_2533723559_59_253: single domain, near sugar dehydrogenase, from Parabacteroides distasonis ATCC 8503 0.992 5848954_85_286: single domain, from Caulobacter crescentus NA1000 5848954_85_2865848954_85_286: single domain, from Caulobacter crescentus NA1000 0.052 Pedo557__CA265_RS22975: single domain 3-ketotrehalose hydrolase from Pedobacter sp. GW460-11-11-14-LB5 Pedo557__CA265_RS22975Pedo557__CA265_RS22975: single domain 3-ketotrehalose hydrolase from Pedobacter sp. GW460-11-11-14-LB5 11045354_54_254: single domain, from Hymenobacter roseosalivarius AA-718, DSM 11622 11045354_54_25411045354_54_254: single domain, from Hymenobacter roseosalivarius AA-718, DSM 11622 11293044_51_238: single domain, from Planctomyces brasiliensis DSM 5305 11293044_51_23811293044_51_238: single domain, from Planctomyces brasiliensis DSM 5305 7398304_63_256: single domain, from Dyadobacter fermentans DSM 18053 (has NEQ)7398304_63_256 single domain, from Dyadobacter fermentans DSM 18053 3723559_59_253: single domain, near sugar dehydrogenase, from Parabacteroides distasonis ATCC 8503 (has NEQ)3723559_59_253 single domain, near sugar dehydrogenase, from Parabacteroides distasonis ATCC 8503 5848954_85_286: single domain, from Caulobacter crescentus NA1000 (has NEQ)5848954_85_286 single domain, from Caulobacter crescentus NA1000 Pedo557__CA265_RS22975: single domain 3-ketotrehalose hydrolase from Pedobacter sp. GW460-11-11-14-LB5 (has NEQ)Pedo557__CA265_RS22975 single domain 3-ketotrehalose hydrolase from Pedobacter sp. GW460-11-11-14-LB5 11045354_54_254: single domain, from Hymenobacter roseosalivarius AA-718, DSM 11622 (has NEQ)11045354_54_254 single domain, from Hymenobacter roseosalivarius AA-718, DSM 11622 11293044_51_238: single domain, from Planctomyces brasiliensis DSM 5305 (has NEQ)11293044_51_238 single domain, from Planctomyces brasiliensis DSM 5305 0.5 /site amino acids:gapgap-alaninealanineAcysteinecysteineCaspartateaspartateDglutamateglutamateEphenylalaninephenylalanineFglycineglycineGhistidinehistidineHisoleucineisoleucineIlysinelysineKleucineleucineLmethioninemethionineMasparagineasparagineNprolineprolinePglutamineglutamineQargininearginineRserineserineSthreoninethreonineTvalinevalineVtryptophantryptophanWtyrosinetyrosineY download SVG

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory