Sites on a Tree

 

Searching for up to 100 curated homologs for 14541 FitnessBrowser__Keio:14541 (604 a.a.)

Found high-coverage hits (≥70%) to 100 curated proteins.

Removed hits that are identical to the query, leaving 98

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

NEPU1_THEVU / Q60053 Neopullulanase 1; Alpha-amylase I; TVA I; EC 3.2.1.135 from Thermoactinomyces vulgaris (see 2 papers)
BAA02471.1 α-amylase I (TVA I) (EC 3.2.1.1) (see protein)
    34% identity, 91% coverage of query (296 bits)

1uh3A Thermoactinomyces vulgaris r-47 alpha-amylase/acarbose complex
    34% identity, 91% coverage of query (295 bits)

2d0fA / Q60053 Crystal structure of thermoactinomyces vulgaris r-47 alpha-amylase 1 (tvai) mutant d356n complexed with p2, a pullulan model oligosaccharide (see paper)
    34% identity, 91% coverage of query (294 bits)

AAO47386.1 cyclodextrinase (EC 3.2.1.54) (see protein)
    35% identity, 87% coverage of query (263 bits)

CDAS_BACSP / Q59226 Cyclomaltodextrinase; CDase; CDase I-5; Cyclomaltodextrin hydrolase, decycling; EC 3.2.1.135; EC 3.2.1.54 from Bacillus sp. (see 4 papers)
AAA92925.1 cyclomaltodextrinase (CDI5;BspCMD) (EC 3.2.1.54) (see protein)
    36% identity, 81% coverage of query (259 bits)

ACC84362.1 debranching enzyme (Npun_R6073) (EC 2.4.1.25|3.2.1.33) (see protein)
    34% identity, 80% coverage of query (254 bits)

CAA44454.1 cyclodextrinase (CDase) (EC 3.2.1.54) (see protein)
    34% identity, 87% coverage of query (253 bits)

AAX29990.1 cyclomaltodextrinase (Cda13;Amy80) (EC 3.2.1.54) (see protein)
    36% identity, 75% coverage of query (251 bits)

NEPU2_THEVU / Q08751 Neopullulanase 2; Alpha-amylase II; TVA II; EC 3.2.1.135 from Thermoactinomyces vulgaris (see 2 papers)
BAA02473.1 cyclomaltodextrinase / neopullulanase (TVA II;TvCMD) (EC 3.2.1.135|3.2.1.54) (see protein)
3a6oA / Q08751 Crystal structure of thermoactinomyces vulgaris r-47 alpha- amylase 2/acarbose complex (see paper)
    35% identity, 75% coverage of query (246 bits)

I6RE37 glucan 1,4-alpha-maltohydrolase (EC 3.2.1.133) from Geobacillus thermoleovorans (see paper)
AFM43699.1 maltogenic-amylase (Amy3;Gt-Mamy) (EC 3.2.1.133) (see protein)
    33% identity, 82% coverage of query (246 bits)

AAC15072.1 cyclomaltodextrinase / maltogenic α-amylase (ThCMD;ThMA) (EC 3.2.1.133|3.2.1.54) (see protein)
    33% identity, 82% coverage of query (245 bits)

AAX85453.1 maltogenic α-amylase (EC 3.2.1.133) (see protein)
    33% identity, 82% coverage of query (245 bits)

E0X988 glucan 1,4-alpha-maltohydrolase (EC 3.2.1.133) from Geobacillus sp. Gh6 (see paper)
    33% identity, 82% coverage of query (244 bits)

CDAS_GEOTM / A0A7U9P668 Cyclomaltodextrinase; CDase; Cyclomaltodextrin hydrolase, decycling; EC 3.2.1.54 from Geobacillus thermopakistaniensis (strain MAS1) (see paper)
    33% identity, 82% coverage of query (244 bits)

1g1yA Crystal structure of alpha-amylase ii (tvaii) from thermoactinomyces vulgaris r-47 and beta-cyclodextrin complex
    35% identity, 75% coverage of query (244 bits)

AGLB_THEMA / Q9X2F4 Cyclomaltodextrinase; Maltodextrin glucosidase; TMG; EC 3.2.1.54 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
AAD36898.1 cyclomaltodextrinase (AglB;TM1835) (EC 3.2.1.54) (see protein)
    36% identity, 76% coverage of query (242 bits)

1gviB / O69007 Thermus maltogenic amylase in complex with beta-cd (see paper)
    33% identity, 82% coverage of query (242 bits)

AAX29991.1 cyclomaltodextrinase (Cda13;Amy98) (EC 3.2.1.54) (see protein)
    31% identity, 83% coverage of query (242 bits)

ACA48225.1 cyclodextrinase (Amy132) (EC 3.2.1.54) (see protein)
    33% identity, 81% coverage of query (241 bits)

AAA23205.1 amylase-pullulanase (Apu) (EC 3.2.1.1|3.2.1.41) (see protein)
    30% identity, 87% coverage of query (238 bits)

1j0hA / P38940 Crystal structure of bacillus stearothermophilus neopullulanase (see paper)
    33% identity, 80% coverage of query (237 bits)

Q9AIV2 neopullulanase (EC 3.2.1.135) from Geobacillus stearothermophilus (see paper)
AAK15003.1 neopullulanase (EC 3.2.1.135) (see protein)
    32% identity, 80% coverage of query (237 bits)

1j0iA Crystal structure of neopullulanase complex with panose
    33% identity, 80% coverage of query (237 bits)

AAA23219.1 cyclodextrinase (CDase) (EC 3.2.1.54) (see protein)
CDase / AAA23219.1 cyclodextrinase from Thermoanaerobacter thermohydrosulfuricus (see paper)
    32% identity, 81% coverage of query (236 bits)

ABY71030.1 oligosaccharide-producing multifunctional amylase (OPMA-N) (EC 3.2.1.135) (see protein)
    34% identity, 75% coverage of query (236 bits)

NEPU_GEOSE / P38940 Neopullulanase; EC 3.2.1.135 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
AAA22622.1 cyclomaltodextrinase / neopullulanase (NplT;BsCMD) (EC 3.2.1.135|3.2.1.54) (see protein)
    33% identity, 80% coverage of query (236 bits)

AAC46346.1 maltogenic α-amylase (BSMA) (EC 3.2.1.133) (see protein)
    31% identity, 83% coverage of query (236 bits)

ACR15165.1 amylopullulanase (Apu;Apu-Ts) (partial) (EC 3.2.1.1|3.2.1.41) (see protein)
    30% identity, 94% coverage of query (235 bits)

AAF23874.1 maltogenic amylase (BbmA) (EC 3.2.1.133) (see protein)
    33% identity, 75% coverage of query (233 bits)

BBMA_BACIU / Q9R9H8 Intracellular maltogenic amylase; EC 3.2.1.- from Bacillus subtilis (see paper)
    33% identity, 75% coverage of query (232 bits)

CAB08035.1 maltogenic α-amylase / neopullulanase (YvdF;BSU34620) (EC 3.2.1.133) (see protein)
    34% identity, 75% coverage of query (231 bits)

BAA31576.1 cyclodextrinase (EC 3.2.1.54) (see protein)
    30% identity, 83% coverage of query (231 bits)

ACA48224.1 neopullulanase (Amy29) (EC 3.2.1.135) (see protein)
    34% identity, 73% coverage of query (231 bits)

apu / AAA19800.2 amylopullulanase, partial from Thermoanaerobacterium saccharolyticum DSM 7060 (see paper)
    31% identity, 89% coverage of query (229 bits)

AAA19800.1 amylase-pullulanase (Apu;Apu-Ts) (EC 3.2.1.1|3.2.1.41) (see protein)
    31% identity, 89% coverage of query (229 bits)

AGLB_THENE / O86959 Cyclomaltodextrinase; Maltodextrin glucosidase; EC 3.2.1.54 from Thermotoga neapolitana (see paper)
CAA08867.1 cyclomaltodextrinase glucanotransferase (MalA;AglB) (EC 3.2.1.54) (see protein)
    34% identity, 76% coverage of query (229 bits)

Q68KL3 glucan 1,4-alpha-maltohydrolase (EC 3.2.1.133) from Bacillus thermoalkalophilus (see paper)
AAT94159.1 maltogenic amylase (BTMA) (EC 3.2.1.133|3.2.1.54) (see protein)
    32% identity, 82% coverage of query (228 bits)

apu / AAA23201.1 amylopullulanase from Thermoanaerobacter thermohydrosulfuricus (see paper)
    31% identity, 87% coverage of query (227 bits)

X5CPN2 glucan 1,4-alpha-maltohydrolase (EC 3.2.1.133) from Alkalihalobacillus lehensis (see 2 papers)
AHW51935.1 maltogenic α-amylase (Mag1;BleG1_0772) (EC 3.2.1.54) (see protein)
    31% identity, 85% coverage of query (227 bits)

APU_THEP3 / P38939 Amylopullulanase; Alpha-amylase/pullulanase; EC 3.2.1.1; EC 3.2.1.41 from Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum) (see paper)
    31% identity, 87% coverage of query (227 bits)

ABY95795.1 amylase-pullulanase (Apu;Apu-Te;TrAPU;Teth39_2173) (EC 3.2.1.1|3.2.1.41) (see protein)
    31% identity, 87% coverage of query (227 bits)

AAB00841.1 amylase-pullulanase (AmyB) (EC 3.2.1.1|3.2.1.41) (see protein)
amyB / AAB00841.1 pullulanase from Thermoanaerobacterium thermosulfurigenes (see 4 papers)
    32% identity, 76% coverage of query (227 bits)

C0LZ63 alpha-amylase (EC 3.2.1.1) from Parageobacillus caldoxylosilyticus (see paper)
ACN79585.1 maltogenic amylase (MA;GcaTK4MA) (EC 3.2.1.133) (see protein)
    31% identity, 82% coverage of query (225 bits)

Build an alignment

Build an alignment for 14541 and 43 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

Or download the sequences

Change minimum %identity:

Additional hits (identity < 30%)

7d9bA Crystal structure of alpha-glucosidase (see paper)
    29% identity, 94% coverage of query (224 bits)

BAA02521.1 neopullulanase (EC 3.2.1.135) (see protein)
    31% identity, 82% coverage of query (223 bits)

7dchA Alpha-glucosidase from weissella cibaria bbk-1 bound with acarbose
    29% identity, 94% coverage of query (223 bits)

7d9cA Alpha-glucosidase from weissella cibaria bbk-1 bound with maltose
    29% identity, 94% coverage of query (223 bits)

CAA80246.1 maltogenic α-amylase (EC 3.2.1.133) (see protein)
    30% identity, 81% coverage of query (221 bits)

ABR26448.1 α-amylase / high-molecular-mass amylase (Hmma;HmwA) (EC 3.2.1.1) (see protein)
    32% identity, 77% coverage of query (220 bits)

A7DWA8 glucan 1,4-alpha-maltohydrolase (EC 3.2.1.133) from Bacillus sp. (in: Bacteria) (see 4 papers)
CAL64396.1 maltogenic amylase (MAUS149) (EC 3.2.1.133) (see protein)
    32% identity, 80% coverage of query (219 bits)

AFI70750.1 amylopullulanase (Apu;Apu105;Gt-Apu) (EC 3.2.1.1|3.2.1.41) (see protein)
    31% identity, 77% coverage of query (219 bits)

AAG44799.1 amylopullulanase (ApuTS) (EC 3.2.1.1|3.2.1.41) (see protein)
    31% identity, 78% coverage of query (214 bits)

cgtB / Q9HHC8 cyclodextrinase (EC 3.2.1.54) from Thermococcus sp. B1001 (see 2 papers)
BAB18100.1 cyclomaltodextrinase (CdnA) (EC 3.2.1.133|3.2.1.54) (see protein)
    32% identity, 73% coverage of query (211 bits)

BAA05832.1 amylase-pullulanase (AapT) (EC 3.2.1.1|3.2.1.41) (see protein)
    31% identity, 77% coverage of query (211 bits)

BAD85166.1 amylopullulanase / pullulanase type III (Pul-Tk;TK-PUL;TK0977) (EC 3.2.1.1|3.2.1.41) (see protein)
    30% identity, 80% coverage of query (210 bits)

CAB94218.1 pullulanase type III (PulhA;Pul-Ta) (EC 3.2.1.41) (see protein)
pulhA / CAB94218.1 pullulan hydrolase type III from Thermococcus aggregans (see paper)
    30% identity, 80% coverage of query (209 bits)

5ot1A / Q5JID9 The type iii pullulan hydrolase from thermococcus kodakarensis (see paper)
    30% identity, 75% coverage of query (207 bits)

BAI67607.1 cyclic maltosyl-maltose hydrolase (CmmF) (EC 3.2.1.-) (see protein)
    31% identity, 76% coverage of query (201 bits)

6a0kA / D2YYE1 Cyclic alpha-maltosyl-(1-->6)-maltose hydrolase from arthrobacter globiformis, complex with panose (see paper)
    31% identity, 76% coverage of query (201 bits)

5zxgA Cyclic alpha-maltosyl-(1-->6)-maltose hydrolase from arthrobacter globiformis, ligand-free form
    31% identity, 76% coverage of query (201 bits)

BAB18516.1 pullulanase (BFLpu) (EC 3.2.1.41) (see protein)
    34% identity, 71% coverage of query (199 bits)

2z1kA / Q5SI17 Crystal structure of ttha1563 from thermus thermophilus hb8
    34% identity, 71% coverage of query (199 bits)

I3ZTQ5 cyclomaltodextrinase (EC 3.2.1.54) from Thermococcus sp. (see paper)
AFL95089.1 cyclomaltodextrinase (CD;TcCD;CL1_0884) (EC 3.2.1.54) (see protein)
    32% identity, 73% coverage of query (197 bits)

ADZ99363.1 α-amylase (AmyA4) (EC 3.2.1.1) (see protein)
    31% identity, 75% coverage of query (196 bits)

ACV59878.1 amylase-pullulanase (Amy;Aaci_2874) (EC 3.2.1.1|3.2.1.41) (see protein)
    30% identity, 75% coverage of query (195 bits)

ACV59873.1 cyclomaltodextrinase (CdaA;Aaci_2869) (EC 3.2.1.54) (see protein)
    32% identity, 71% coverage of query (193 bits)

Q9HHB0 pullulanase (EC 3.2.1.41) from Desulfurococcus mucosus (see paper)
AAG31033.1 pullulanase (ApuA;Pul-Dm;Desmu_0653) (EC 3.2.1.41) (see protein)
    27% identity, 92% coverage of query (190 bits)

ABA39290.1 cyclomaltodextrinase (CdaA) (Cda13A) (EC 3.2.1.54) (see protein)
    28% identity, 77% coverage of query (187 bits)

ZP_00047084.1 maltogenic α-amylase (LgMA;Lgas02001568) (EC 3.2.1.133) (see protein)
ABJ59624.1 maltogenic α-amylase (Lgma;LGAS_0215) (EC 3.2.1.133) (see protein)
    29% identity, 80% coverage of query (187 bits)

BAN63122.1 α-amylase (AmyL) (EC 3.2.1.1) (see protein)
    31% identity, 78% coverage of query (184 bits)

ACJ17286.1 cyclomaltodextrinase (Tocd;TON_1796) (EC 3.2.1.54) (see protein)
    30% identity, 73% coverage of query (182 bits)

ACA48223.1 cyclodextrinase (Amy1) (EC 3.2.1.54) (see protein)
    30% identity, 82% coverage of query (181 bits)

A7LI67 neopullulanase (EC 3.2.1.135) from uncultured microorganism (see paper)
ABS59068.1 Npu193A (EC 3.2.1.-) (see protein)
    29% identity, 75% coverage of query (180 bits)

Q5JJ59 cyclomaltodextrinase (EC 3.2.1.54) from Thermococcus kodakarensis (see 2 papers)
BAD85959.1 cyclodextrinase (CDase-Tk;TK1770) (EC 3.2.1.54) (see protein)
    30% identity, 80% coverage of query (176 bits)

ABL78855.1 maltogenic α-amylase / cyclomaltodextrinase (TfMA;Tpen_1458) (EC 3.2.1.133|3.2.1.54) (see protein)
    30% identity, 77% coverage of query (176 bits)

AGT54942.1 α-amylase (Amy703;Amy) (EC 3.2.1.1) (see protein)
    30% identity, 78% coverage of query (171 bits)

Q8TZP8 cyclomaltodextrinase (EC 3.2.1.54) from Pyrococcus furiosus (see paper)
AAL82063.1 α-amylase / cyclomaltodextrinase (PFTA;PF1939) (Amy13) (EC 3.2.1.1|3.2.1.54) (see protein)
    27% identity, 85% coverage of query (168 bits)

A3DM60 glucan 1,4-alpha-maltohydrolase (EC 3.2.1.133) from Staphylothermus marinus (see 2 papers)
ABN69720.1 maltogenic α-amylase / cyclomaltodextrinase (MA;SmMA;Smar_0613) (EC 3.2.1.133|3.2.1.54) (see protein)
    25% identity, 80% coverage of query (158 bits)

N515DRAFT_3391 Alpha-amylase (EC 3.2.1.1) from Dyella japonica UNC79MFTsu3.2
    27% identity, 70% coverage of query (140 bits)

BAA07401.1 α-amylase (EC 3.2.1.1) (see protein)
    27% identity, 70% coverage of query (138 bits)

AAW32490.1 α-amylase (AmyB) (EC 3.2.1.1) (see protein)
    28% identity, 73% coverage of query (136 bits)

Echvi_2850 Alpha-amylase (EC 3.2.1.1) from Echinicola vietnamensis KMM 6221, DSM 17526
    24% identity, 75% coverage of query (135 bits)

CAA31586.1 α-amylase 2 (AmyB) (EC 3.2.1.1) (see protein)
    24% identity, 75% coverage of query (134 bits)

AAQ89599.1 α-amylase (AmyM) (EC 3.2.1.1) (see protein)
    24% identity, 71% coverage of query (132 bits)

ADF53136.1 α-amylase (AmyZ;ZPR_2816) (EC 3.2.1.1) (see protein)
    26% identity, 76% coverage of query (124 bits)

A0A0U4VEK2 alpha-amylase (EC 3.2.1.1) from Paenibacillus sp. SSG-1 (see paper)
    28% identity, 70% coverage of query (122 bits)

6y9tA / Q5FI02 Family gh13_31 enzyme (see paper)
    23% identity, 70% coverage of query (114 bits)

6wniA / U5CJP3 Crystal structure of clda, the first cyclomaltodextrin glucanotransferase with a three-domain abc distribution
    24% identity, 80% coverage of query (112 bits)

Q47SE5 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Thermobifida fusca (see paper)
    26% identity, 71% coverage of query (112 bits)

CBK89424.1 maltogenic α-amylase (EUR_01860) (EC 3.2.1.133) (see protein)
    23% identity, 70% coverage of query (111 bits)

cgtA / Q9UWN2 cyclomaltodextrin glucanotransferase (EC 2.4.1.19) from Thermococcus sp. B1001 (see 6 papers)
Q9UWN2 cyclomaltodextrin glucanotransferase (EC 2.4.1.19) from Thermococcus sp. (see 3 papers)
BAA88217.1 α-cyclodextrin glucanotransferase (CgtA) (EC 2.4.1.19) (see protein)
    26% identity, 71% coverage of query (109 bits)

7jjnA Eubacterium rectale amy13b (eur_01860)
    23% identity, 70% coverage of query (109 bits)

BT3704 SusA: alpha-amylase / neopullulanase / cyclomaltodextrinase (EC 3.2.1.135; EC 3.2.1.54) from Bacteroides thetaiotaomicron VPI-5482
SUSA_BACTN / Q8A1G0 Neopullulanase SusA; Starch-utilization system protein A; EC 3.2.1.135 from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) (see paper)
AAO78809.1 neopullulanase (SusA;BT3704;BT_3704) (EC 3.2.1.135) (see protein)
    24% identity, 77% coverage of query (103 bits)

Q8X268 cyclomaltodextrin glucanotransferase (EC 2.4.1.19) from Thermococcus kodakarensis (see 2 papers)
BAB78538.1 β-cyclodextrin glucanotransferase (Cgt;TK-cgt;TK2172) (EC 2.4.1.19) (see protein)
    26% identity, 71% coverage of query (101 bits)

D1ABU6 maltose alpha-D-glucosyltransferase (EC 5.4.99.16) from Thermomonospora curvata (see 2 papers)
    24% identity, 71% coverage of query (98.6 bits)

3zoaA The structure of trehalose synthase (tres) of mycobacterium smegmatis in complex with acarbose
    24% identity, 72% coverage of query (96.7 bits)

3zo9A The structure of trehalose synthase (tres) of mycobacterium smegmatis
    24% identity, 72% coverage of query (96.7 bits)

4jcmA / B9A1J6 Crystal structure of gamma-cgtase from alkalophilic bacillus clarkii at 1.65 angstrom resolution
    24% identity, 73% coverage of query (92.8 bits)

Or start over

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory