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Searching for up to 100 curated homologs for 14729 FitnessBrowser__Keio:14729 (391 a.a.)

Found high-coverage hits (≥70%) to 28 curated proteins.

Removed hits that are identical to the query, leaving 26

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

5jxzA A low magnesium structure of the isochorismate synthase, entc
    99% identity, 96% coverage of query (759 bits)

5jy8A An iron-bound structure of the isochorismate synthase entc
    98% identity, 96% coverage of query (747 bits)

vibC / O07898 vibriobactin-specific isochorismate synthase (EC 5.4.4.2) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
    39% identity, 99% coverage of query (244 bits)

dhbC / P45744 isochorismate synthase (EC 5.4.4.2) from Bacillus subtilis (strain 168) (see paper)
    37% identity, 95% coverage of query (236 bits)

P45744 Isochorismate synthase DhbC; Isochorismate mutase; EC 5.4.4.2 from Bacillus subtilis (strain 168)
    37% identity, 95% coverage of query (236 bits)

Build an alignment

Build an alignment for 14729 and 5 homologs with ≥ 30% identity

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Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

pchA / Q51508 isochorismate synthase (EC 5.4.4.2) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
PCHA_PSEAE / Q51508 Salicylate biosynthesis isochorismate synthase; Isochorismate mutase; EC 5.4.4.2 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
    29% identity, 93% coverage of query (145 bits)

ICS_CATRO / Q9ZPC0 Isochorismate synthase, chloroplastic; EC 5.4.4.2 from Catharanthus roseus (Madagascar periwinkle) (Vinca rosea) (see 3 papers)
    28% identity, 70% coverage of query (114 bits)

trpE / AAA25223.1 anthranilate synthase alpha subunit from Lactococcus lactis (see paper)
    26% identity, 80% coverage of query (104 bits)

P9WFX2 anthranilate synthase (EC 4.1.3.27) from Mycobacterium tuberculosis (see paper)
P9WFX3 anthranilate synthase (EC 4.1.3.27) from Mycobacterium tuberculosis (see paper)
    30% identity, 76% coverage of query (94.7 bits)

5cwaA / P9WFX2 Structure of anthranilate synthase component i (trpe) from mycobacterium tuberculosis with inhibitor bound (see paper)
    30% identity, 76% coverage of query (94.7 bits)

8qc4A Salicylate synthase
    28% identity, 71% coverage of query (63.2 bits)

MBTI_MYCTU / P9WFX1 Salicylate synthase; Chorismate mutase; CM; Isochorismate synthase/isochorismate lyase; Mycobactin synthase protein; EC 5.4.99.5; EC 4.2.99.21; EC 5.4.4.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 8 papers)
P9WFX1 isochorismate lyase (EC 4.2.99.21); isochorismate synthase (EC 5.4.4.2) from Mycobacterium tuberculosis (see 2 papers)
    28% identity, 71% coverage of query (63.2 bits)

6za5B M. Tuberculosis salicylate synthase mbti in complex with salicylate and mg2+
    28% identity, 71% coverage of query (63.2 bits)

3vehB / P9WFX1 Structure of a m. Tuberculosis salicylate synthase, mbti, in complex with an inhibitor methylamt (see paper)
    28% identity, 71% coverage of query (62.8 bits)

3logB Crystal structure of mbti from mycobacterium tuberculosis
    28% identity, 71% coverage of query (62.8 bits)

2g5fA The structure of mbti from mycobacterium tuberculosis, the first enzyme in the synthesis of mycobactin, reveals it to be a salicylate synthase
    28% identity, 71% coverage of query (62.8 bits)

3st6A Structure of a m. Tuberculosis synthase, mbti, in complex with an isochorismate analogue inhibitor
    28% identity, 71% coverage of query (62.8 bits)

3rv8C Structure of a m. Tuberculosis salicylate synthase, mbti, in complex with an inhibitor with cyclopropyl r-group
    28% identity, 71% coverage of query (61.6 bits)

3rv7A Structure of a m. Tuberculosis salicylate synthase, mbti, in complex with an inhibitor with isopropyl r-group
    28% identity, 71% coverage of query (60.8 bits)

6za4A M. Tuberculosis salicylate synthase mbti in complex with 5-(3- cyanophenyl)furan-2-carboxylate
    28% identity, 71% coverage of query (60.5 bits)

3rv9A Structure of a m. Tuberculosis salicylate synthase, mbti, in complex with an inhibitor with ethyl r-group
    28% identity, 71% coverage of query (57.8 bits)

3rv8A Structure of a m. Tuberculosis salicylate synthase, mbti, in complex with an inhibitor with cyclopropyl r-group
    27% identity, 71% coverage of query (56.6 bits)

3rv6B Structure of a m. Tuberculosis salicylate synthase, mbti, in complex with an inhibitor with phenyl r-group
    27% identity, 71% coverage of query (54.7 bits)

3rv6A Structure of a m. Tuberculosis salicylate synthase, mbti, in complex with an inhibitor with phenyl r-group
    28% identity, 71% coverage of query (54.3 bits)

3rv9C Structure of a m. Tuberculosis salicylate synthase, mbti, in complex with an inhibitor with ethyl r-group
    27% identity, 71% coverage of query (52.0 bits)

6za4B / P9WFX1 M. Tuberculosis salicylate synthase mbti in complex with 5-(3- cyanophenyl)furan-2-carboxylate (see paper)
    27% identity, 71% coverage of query (48.9 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory