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Searching for up to 100 curated homologs for 15418 FitnessBrowser__Keio:15418 (254 a.a.)

Found high-coverage hits (≥70%) to 16 curated proteins.

Removed hits that are identical to the query, leaving 14

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

BWI76_RS10705 Gamma-glutamyl-gamma-aminobutyrate hydrolase (EC 3.5.1.94) from Klebsiella michiganensis M5al
    88% identity, 100% coverage of query (464 bits)

SO1267 Gamma-glutamyl-gamma-aminobutyrate hydrolase (EC 3.5.1.94) from Shewanella oneidensis MR-1
    44% identity, 97% coverage of query (196 bits)

AO356_13140 Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94) from Pseudomonas fluorescens FW300-N2C3
    44% identity, 96% coverage of query (192 bits)

7d50B / Q9I6J4 Spua mutant - h221n with glutamyl-thioester (see paper)
    44% identity, 96% coverage of query (192 bits)

7d53A Spua mutant - h221n with glu
    44% identity, 96% coverage of query (192 bits)

Pf6N2E2_4510 Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94) from Pseudomonas fluorescens FW300-N2E2
    45% identity, 92% coverage of query (184 bits)

Sama_2645 Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94) from Shewanella amazonensis SB2B
    43% identity, 97% coverage of query (183 bits)

Sama_0794 gamma-glutamyl-gamma-aminobutyrate hydrolase (EC: 3.5.1.94) from Shewanella amazonensis SB2B
    40% identity, 94% coverage of query (174 bits)

AO353_29305 Gamma-glutamyl-GABA hydrolase (EC 3.5.1.94) from Pseudomonas fluorescens FW300-N2E3
    44% identity, 96% coverage of query (173 bits)

7d4rB / Q9I6J4 Spua native structure (see paper)
    41% identity, 93% coverage of query (161 bits)

3fijA / Q92AL3 Crystal structure of a uncharacterized protein lin1909
    36% identity, 93% coverage of query (120 bits)

YHE5_SCHPO / Q9HDV0 Putative glutamine amidotransferase PB2B2.05 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
    35% identity, 89% coverage of query (103 bits)

O33341 Putative glutamine amidotransferase Rv2859c; EC 2.4.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
    30% identity, 92% coverage of query (86.7 bits)

Build an alignment

Build an alignment for 15418 and 13 homologs with ≥ 30% identity

Select sequences

Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

GT121_ARATH / Q8H0Z4 Putative glutamine amidotransferase GAT1_2.1; Class I glutamine amidotransferase; GAT1_2 domain-containing protein 1; EC 2.4.2.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
    26% identity, 93% coverage of query (79.0 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory