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Searching for up to 100 curated homologs for 16277 FitnessBrowser__Keio:16277 (312 a.a.)

Found high-coverage hits (≥70%) to 39 curated proteins.

Removed hits that are identical to the query, leaving 38

You can add additional sequences or change the %identity threshold for inclusion. Once you have selected sequences, you can build an alignment and a tree.

Hits with ≥ 30% identity

K1PF_VIBCH / Q9KM71 1-phosphofructokinase; Fructose 1-phosphate kinase; Fru1PK; EC 2.7.1.56 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see 2 papers)
    50% identity, 99% coverage of query (302 bits)

Psest_3354 1-phosphofructokinase (EC 2.7.1.56) from Pseudomonas stutzeri RCH2
    45% identity, 99% coverage of query (244 bits)

AO356_07330 1-phosphofructokinase (EC 2.7.1.56) from Pseudomonas fluorescens FW300-N2C3
    43% identity, 99% coverage of query (234 bits)

K1PF_XANCP / P23354 1-phosphofructokinase; Fructose 1-phosphate kinase; Fru1PK; EC 2.7.1.56 from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) (see paper)
    40% identity, 90% coverage of query (148 bits)

G4SW45 diphosphate-fructose-6-phosphate 1-phosphotransferase (EC 2.7.1.90) from Methylotuvimicrobium alcaliphilum (see paper)
    34% identity, 81% coverage of query (120 bits)

Build an alignment

Build an alignment for 16277 and 5 homologs with ≥ 30% identity

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Add sequences from UniProt, PDB, RefSeq, or MicrobesOnline (separate identifiers with commas or spaces):

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Change minimum %identity:

Additional hits (identity < 30%)

LACC_LACLL / P23391 Tagatose-6-phosphate kinase; Phosphotagatokinase; EC 2.7.1.144 from Lactococcus lactis subsp. lactis (Streptococcus lactis) (see paper)
    28% identity, 98% coverage of query (114 bits)

2jgvB Structure of staphylococcus aureus d-tagatose-6-phosphate kinase in complex with adp
    27% identity, 94% coverage of query (114 bits)

2jg1A / P0A0B9 Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
    27% identity, 94% coverage of query (113 bits)

2jg1C Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate
    27% identity, 94% coverage of query (113 bits)

K1PF_HALVD / D4GYE6 1-phosphofructokinase; Fructose 1-phosphate kinase; Fru1PK; EC 2.7.1.56 from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
D4GYE6 1-phosphofructokinase (EC 2.7.1.56) from Haloferax volcanii (see paper)
    34% identity, 93% coverage of query (112 bits)

lacC / P0A0B8 D-tagatose-6-phosphate kinase subunit (EC 2.7.1.144) from Staphylococcus aureus (strain MW2) (see 2 papers)
P0A0B9 tagatose-6-phosphate kinase (EC 2.7.1.144) from Staphylococcus aureus (see paper)
    27% identity, 93% coverage of query (112 bits)

2f02A / Q833W9 Crystal structure of lacc from enterococcus faecalis in complex with atp
    30% identity, 84% coverage of query (112 bits)

3uqeA Crystal structure of the phosphofructokinase-2 mutant y23d from escherichia coli
    32% identity, 97% coverage of query (108 bits)

PfkB / b1723 6-phosphofructokinase 2 (EC 2.7.1.11; EC 2.7.1.144) from Escherichia coli K-12 substr. MG1655 (see 7 papers)
pfkB / P06999 6-phosphofructokinase 2 (EC 2.7.1.11; EC 2.7.1.144) from Escherichia coli (strain K12) (see 40 papers)
PFKB_ECOLI / P06999 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; 6-phosphofructokinase isozyme II; Phosphohexokinase 2; EC 2.7.1.11 from Escherichia coli (strain K12) (see 4 papers)
P06999 6-phosphofructokinase (EC 2.7.1.11) from Escherichia coli (see 2 papers)
3n1cA / P06999 Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with fructose-6-phosphate (see paper)
    33% identity, 90% coverage of query (107 bits)

3uqdB Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products
    33% identity, 90% coverage of query (107 bits)

3uqdA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products
    33% identity, 90% coverage of query (107 bits)

3cqdA Structure of the tetrameric inhibited form of phosphofructokinase-2 from escherichia coli
    33% identity, 90% coverage of query (106 bits)

3ie7A / Q929S5 The crystal structure of phosphofructokinase (lin2199) from listeria innocua in complex with atp at 1.6a
    29% identity, 92% coverage of query (105 bits)

3julA Crystal structure of listeria innocua d-tagatose-6-phosphate kinase bound with substrate
    29% identity, 91% coverage of query (100 bits)

2ajrA / Q9WZT5 Crystal structure of possible 1-phosphofructokinase (ec 2.7.1.56) (tm0828) from thermotoga maritima at 2.46 a resolution
    28% identity, 97% coverage of query (99.0 bits)

Q8VS15 D-tagatose-1-phosphate kinase (EC 2.7.1.234) from Klebsiella oxytoca (see paper)
    26% identity, 94% coverage of query (97.8 bits)

tagK / Q65EY9 D-tagatose-1-phosphate kinase (EC 2.7.1.234) from Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46) (see 3 papers)
Q65EY9 D-tagatose-1-phosphate kinase (EC 2.7.1.234) from Bacillus licheniformis (see paper)
    28% identity, 92% coverage of query (94.7 bits)

PFKB_MYCTO / P9WID2 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; Phosphofructokinase B; Phosphohexokinase 2; Tagatose-6-phosphate kinase; EC 2.7.1.11; EC 2.7.1.144 from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) (see paper)
PFKB_MYCTU / P9WID3 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; Phosphofructokinase B; Phosphohexokinase 2; Tagatose-6-phosphate kinase; EC 2.7.1.11; EC 2.7.1.144 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 6 papers)
    29% identity, 90% coverage of query (79.7 bits)

K1PF_MYCGE / Q49396 Putative 1-phosphofructokinase; Fructose 1-phosphate kinase; Fru1PK; EC 2.7.1.56 from Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37) (Mycoplasmoides genitalium) (see paper)
    25% identity, 86% coverage of query (77.8 bits)

1tz3A / Q8ZKR2 Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
    28% identity, 79% coverage of query (65.9 bits)

1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog
    28% identity, 83% coverage of query (65.9 bits)

3in1A / P77493 Crystal structure of a putative ribokinase in complex with adp from e.Coli
    27% identity, 81% coverage of query (58.5 bits)

YdjH / b1772 L-glycero-L-galacto-octuluronate kinase from Escherichia coli K-12 substr. MG1655 (see 2 papers)
ydjH / P77493 L-glycero-L-galacto-octuluronate kinase from Escherichia coli (strain K12) (see 2 papers)
    27% identity, 81% coverage of query (58.5 bits)

Q9YG89 6-phosphofructokinase (EC 2.7.1.11) from Aeropyrum pernix (see 2 papers)
    27% identity, 84% coverage of query (57.4 bits)

YegV / b2100 putative sugar kinase YegV from Escherichia coli K-12 substr. MG1655 (see paper)
    28% identity, 83% coverage of query (56.2 bits)

KDGK_BACSU / P50845 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Bacillus subtilis (strain 168) (see paper)
    27% identity, 90% coverage of query (53.5 bits)

RBSK_LEIMA / E9AD19 Ribokinase; RK; EC 2.7.1.15 from Leishmania major (see paper)
    23% identity, 88% coverage of query (52.0 bits)

6a8aA / A0A3S7X0F5 Ribokinase from leishmania donovani with atp (see paper)
    24% identity, 88% coverage of query (50.1 bits)

6a8cA Ribokinase from leishmania donovani with adp
    24% identity, 88% coverage of query (50.1 bits)

6a8bA Ribokinase from leishmania donovani with amppcp
    24% identity, 88% coverage of query (50.1 bits)

BPHYT_RS02045 Fructokinase (EC 2.7.1.4) from Burkholderia phytofirmans PsJN
    27% identity, 75% coverage of query (49.3 bits)

AO356_00950 Ribokinase (EC 2.7.1.15) from Pseudomonas fluorescens FW300-N2C3
    26% identity, 93% coverage of query (49.3 bits)

Pf1N1B4_6031 Ribokinase (EC 2.7.1.15) from Pseudomonas fluorescens FW300-N1B4
    26% identity, 93% coverage of query (46.2 bits)

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by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory